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USER GUIDE
Version 1.1
Dilor-Jobin Yvon-Spex
All Rights Reserved
Printed in France
MM-NP/Labdoc.FM/0399
August 1999
ISA Inc. 3880 Park Avenue, EDISON - New Jersey 08820 (USA)
Tel: (1) 908 549 71 44 - Fax: (1) 908 549 51 25
ISA ITALIA Srl Via Cesare Pavese 35/AB - 20090 OPERA (Milano) (Italy)
Tel: (39) 2 57 60 30 50 - Fax (39) 2 57 60 08 76
With its long experience in research and manufacture of scientific instruments (since 1819), the Dilor-
Jobin Yvon-Spex equipment, division of HORIBA Group, uses state of art technology for optical,
mechanical, electronic and computer management.
The result is unequaled comfort and flexibility of use, with less human error, for the kind of precision
and reproducibility of measurements that are required in laboratory work today.
Last but not least, Dilor-Jobin Yvon-Spex instruments are designed and manufactured with total user
safety in mind.
Dilor-Jobin Yvon-Spex, HORIBA Group division, guarantees each instrument it manufactures for a
period of one (1) year, including parts and labor. Our obligations during this period will be limited to
the repair or replacement -at our discretion- of every instrument returned to the factory, shipment pre-
paid, during a period of one (1) year from the date of delivery to the original purchaser, providing that
Dilor-Jobin Yvon-Spex or its representative gives prior approval.
Your approach to the LabSpec User Guide depends on what you want to do and how much you already
know.
The following list gives a brief description of each section of this manual.
1. Introduction
2. Reference section
4. Tips section
5. Index
Icons list . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
LabSpec Software 9
Icons list
LabSpec Software 10
14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 46
LabSpec Software
30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45
1
2
3
4
5
6
7
8
9
10
47
11
12
13
11
Icons list
Filter: There are 6 neutral filters with the optical densities 0.3, 0.6, 1, 2, 3 and 4.
filter [---] = no attenuation (P0), [D0.3] = P0/2, [D0.6] = P0/4, [D1] = P0/10,
[D2] = P0/100, [D3] = P0/1000 and [D4] = P0/10000
Options:
: 1800g/mm and 300, 600 or 1200 g/mm. Be careful to initialize spectrograph if the grating
is changed.
: Enter a name for the next recorded spectrum. The program will add an incremental number
after the entered name. Be careful to give no more than 8 characters.
LabSpec Software 12
Icon 1 Standard cursor for working
Allows to come back to the standard cursor after having selected one of the other tools that are
presented by the icons 2 to 11. This tool allows to move the different cursor found in the acqui-
sition window.
This feature permits to modify a spectrum by moving each point of the spectrum.
Icon 4 Zoom
Press the left button of the mouse and draw a rectangle which select the part of the spectrum to
zoom.
Icon 8 Shift
This option allows the user to add a constant to the active spectrum.
This is a manual method, using the mouse:
1 Press the mouse left button +Const ,
2 Move the spectrum up and down, then press OK to validate.
LabSpec Software 13
Icons list
This option allows the user to multiply the active spectrum by a constant.
This is a manual method, using the mouse.
The procedure is the same as the one for manual addition.
The wavelength or wavenumber of a peak appears if the left button of the mouse is pressed.
The label will be deleted by pressing the right button of the mouse.
By pressing the left button of the mouse, the label of a peak can be moved precisely. The label
selected is in white, by default it is the last one, but another color can be selected in the window
Bands Parameters (icon 45 then button Bands)
By pressing the left button of the mouse, the width of a band can be changed. As for the icon
number 5, the bandwidth in the window Bands parameters can be selected (icon 45 then
button bands)
Icon 14 Delete
Delete the active object (e.g. acquired spectrum) in the active window.
Icon 15 Load
Load from the disk. The appropriate previously saved file will automatically be opened.
The active object like an acquired spectrum can be saved on the hard disk.
SPECTRA.
When a spectrum is acquired, it is not saved but remains in the Random Access Memory
(RAM) of the computer.
If several spectra are acquired, different color dots will be displayed on the right edge of the
window. Each dot takes the same color as the displayed spectrum and each dot works as a
radio toggle button. Each time an acquisition is performed a new color button is added.The
full names of the spectra are indicated in the object menu command.
LabSpec Software 14
The active object like an acquired spectrum can be saved on the hard
To save one of the displayed spectra, activate the spectrum window, select the desired spec-
trum by pressing the radio button, then choose one of the following methods
1- Click on the following icon: then enter the file name, its location and format.
2- From the file Main Menu, select the Save As option, then enter the file name, its
location and format.
Before saving, it would be useful to complete the information list about the spectrum (operator,
laser power, etc...) by pressing the icon . Some parameters (hole, slit, spectro, grating, time,
accum, date) are automatically updated.
The following is a list of the various file formats that LabSpec supports to save the acquired
spectra or images:
- Dilor format (*. ms0): This format can be used with the other DILOR softwares.
- Text format (*.txt): This is an ASCII mode format which uses two columns: wave-
length/Wavenumber and intensity, without header.
- Spectra Calc format (*.spc), This format is used by SpectraCalc and Grams process-
ing softwares.
IMAGES.
The save procedure always saves the content of the active window. To save an image, acti-
vate the window that contains all the spectra. If the single spectrum window choise is acti-
vated, only this spectrum will be saved, and not the whole spectral image.
To save a video image, activate the window that contains the video image.
LabSpec Software 15
Icons list
Bring cursor to the center of the active window. Sometimes the cursor is not visible, in this case
move the mouse.
Icon 18 Normalization
Video images, spectral images or spectra can be inserted and printed on a prepared layout page.
There is another way to print the data: transfer them to another Windows 95/Windows 98
software using the copy pictures or copy data choices from the EDIT menu.
This Print icon prints out the objects of the active window
The layout page can be configured with the option print set up from the EDIT menu.
LabSpec Software 16
Icon 22 Mathematical processing
When several regions have been acquired, these regions can be glued with the Multi-Window
Option feature. The result is displayed in a new window and the previous separate regions are
not deleted.
Example:
The figure above gives the example of a 3 windows acquisition, the first one covers 200-500
cm-1, the second one 1200-1800 cm-1 and the third 2300-2700 cm-1.
If the whole spectrum between 200 and 3000 cm-1 must be acquired, select the first line and
the numbers 200 and 3000 in the box Window limits .
If the combine feature has not been validated before the acquisition, it is possible to perform it
after an acquisition by pressing the button COMBINE ; once activated, it will combine all
the spectra of the active window.
These mathematical procedures can be applied to spectra, spectral profiles, spectral images and
Video images.
Available calculations:
- Calculate a function of the active spectrum,
- Add a constant to a spectrum,
- Multiply a spectrum by a constant,
- Add, subtract, multiply, divide two spectra,
LabSpec Software 17
Icons list
A:
This option adds a constant to a spectrum. Enter the constant value in the + field, then press
the button OK to validate.
This option adds a constant to the spectrum. First, enter the value of the constant in the first
field. Then, press the button OK to validate.
These four buttons allows the user to add, subtract, multiply or divide two spectra.
The first spectrum is the current active spectrum but the program will ask for the second spec-
trum, if more than two spectra are displayed in the window.
LabSpec Software 18
Icon 22 Mathematical processing
The spectra baseline can be computed and subtracted by pressing this button
The main procedure is to build up point by point a baseline, to fit at best to the spectrum
and then subtract.
.
LabSpec Software 19
Icons list
An automatic procedure can also be used for the computation of the baseline, button
AUTO .
1- Ins/Del:
It permits to select the points for the computation of baseline. Validate the point with the left
button of the mouse or deletes it with the right button.
2- +Const:
Add a constant to the baseline. Validate this button and move the baseline with the cursor.
3- *Const:
Multiply the baseline by a constant. The procedure is the same as the one for +Const.
4- Zoom:
It allows to magnify a spectrum zone.
LabSpec Software 20
Icon 24 Correction
B: Deviation
Choose the deviation (1% is normally appropriate): that is to say the residual difference
between the calculated baseline and the optimal one.
The automatic calculation of baseline depends on this coefficient.
C: Attachment
A best fitting can be done automatically with this command. It attaches the baseline
to the spectrum. It could be useful for the correction of baseline of spectral images.
Icon 24 Correction
When this option is chosen, the following window appears and gives access to some utilities
for the correction of spectra and spectral images.
GET: The active spectrum in the single spectrum window can be taken as a reference for
the options normalization , sub , add , div , mul and corr .
The reference spectrum is stored in a correction window . This spectrum is removed with
the command del
THR
This option is used for the images. For every spectrum of an image, this function activates to
zero all the intensity values that are lower than thr(%) of the intensity of the principal peak.
This function allows to remove the lowest peaks
Threshold(%)
It is the limit which is expressed in percentage of the value for the threshold for the elimination
of weak peaks.
LabSpec Software 21
Icons list
ZERO
Translate the spectrum or all the spectra until the lower point is brought to zero.
Normalization
Normalization of the spectra to the part of corrector spectrum that is inside the green cursors in
the correction window
These options allows the user to multiply, subtract, add or divide the corrector spectrum to all
the spectra of a spectral image.
Spectrum correction
LabSpec Software 22
Icon 24 Correction
Icon 25 Profile
This window allows the user to glue spectra together in a 3D array, change or insert spectrum
in a set of spectra.
A profile of a 2D image (CCD image, video image, mapping...) can also be performed
Profile
To profile an image, select the cursor line on this image (if a horizontal line has been chosen,
do not forget to check in the HOR box).Then press PROFILE .
A rectangular cursor can be also selected to start profile generation.
Insert
Insert the active spectrum in a set of spectra. With the cursor, choose the position of the spec-
trum in the image.
Remove
LabSpec Software 23
Icons list
Change
LabSpec Software 24
Icon 24 Correction
Add
Extract profile
LabSpec Software 25
Icons list
Icon 26 Filtering
The Filtering feature can be applied to any data object like spectrum, image, spectrum
profile, spectral images ...
Each of these data is a multidimensional cube and each of these has its own specific dimension
defines as follow:
Spectrum: one dimension (wave number),
Image: two dimensions (X and Y axis)
Spectrum profile: two dimensions (time and wave number)
Spectral image: three dimensions (X, Y and wave number)
...
To perform a filtering at least one dimension must be selected.
Load customized
filtering
Launch filtering
LabSpec Software 26
Icon 24 Correction
Size: Size of the mask (the number of points around the filtered central datum that are involved
in calculations of the filtered value)
Dimension: As explain above, dimension is the parameter on which is applied the filtering
process. As an example, if you want to filter a spectrum, the dimension will be F (wave
number). For an image, the dimensions available are F , X or Y . If a wave number
filtering is asked for a spectral image, all the spectra will be filtered.
Symmetry: You can choose in the box symmetry if the weight of the points of the mask are
symmetrical in respect to the point to be filtered or not.
- Ordered:
Once the mask characteristics has been chosen, the software analyzes the data levels (intensi-
ties) located in the mask then replace the central datum level (intensity) by the level determines
using one of the following options:
- MAX: the maximum level value finded in the mask,
- MIN: the minimum level value finded in the mask,
- MED: the middle level value,
- INC: the intermediate value between the maximum value and middle value,
- DEC: the intermediate value between the minimum value and the middle
value.
- Average :
Choosing this option will substitute the selected datum value (0 in the mask) by the average
value calculates from all of the values of the mask. The calculation process uses the following
formula:
n
y i
i=1
yc =
n
- PeakElm:
The PeakElm option modifies the spectrum only if a sharp peak is found. A peak is detected
if its intensity is higher than the enterd threshold value. The level of the threshold must be
enterd in the PEAK field. A value of one means that the threshold is fixed at 100% of the
average value calculated from mask values, 2 means 200% etc...
If I is the intensity of the point to modify and Im the average intensity of the mask points,
then the value of I is replaced by Im if I<Im+I*peak
LabSpec Software 27
Icons list
- Linear:
The Linear option performs a linear combination of the values of the mask. The coefficients
(wi) of the linear combination can be introduced directly in the mask. In this case the value of
yc is:
w y i i
yc = i =1
n
wi
i =1
Special types of filtering can be saved and loaded again with LOAD button.
A major example of linear filtering is the derivative. In this case, the mask will contain the
coefficients 1, -1, 0 or also 1, 0, -1 if derivative is performed on 3 pixels.
- Polyn:
Polynomial filtering is only available for one-dimensional filtering. The degree of the polynom
interpolates the values in the mask. Enter the degree value in the ORDER field.
LabSpec Software 28
Icon 24 Correction
This option allows the calculation of the Inverse Fourier Transform of a spectrum.
It can also be used for images.
By applying mathematical functions to the interferogram, it is possible to correct some defaults
of the spectrum
Dimension: Choose the dimension where you want to calculate the IFT (inverse Fourier trans-
form) (Freq. for a spectrum, X or Y for an image)
Remark: the parameters of the different correction equations can be easily modified by adjust-
ing graphically their curves with the mouse
DFT: After a modification of the interferogram this icon allows you to calculate the Fourier
transform and modify the spectrum of the active window
LabSpec Software 29
Icons list
The Peak Fitting feature detects the peaks using different shaped bands, like gaussian/lorentz-
ian or even custom ones.
1. AUTO:
The automatic detection of peaks is obtained by pressing the button SEARCH .You must
take care of the position of HEIGHT an NEIGHBOUR scrolling bars.
ZONE = (f1-f2)*bar%
2. Icon n 11
The label button allows you to put the label of the different peaks by using the icon .
LabSpec Software 30
Icon 24 Correction
The sum button displays the sum of all bands, i.e. the fitting.
With the button attach , the label can be fixed to the related peak.
BANDS: In the window BANDS we have the result of the peak fitting procedure. (This list
of results can be saved with the icon SAVE)
For the images, if you want to have the fitting of the position, the surface, the amplitude or
the width at half maximum, in the Bands window , activate the buttons p , s , a or
w .
LabSpec Software 31
Icons list
FUNCTION:
- Gaussian/Lorentzian
y = a*(g*exp(-((x-p)*(x-p))/(w*w))+(1-g)/(1+4*((x-p)*(x-p))/(w*w))
- Gaussian
y = a*exp(-((x-p)*(x-p))/(w*w))
- Lorentzian
y = a/(1+4*((x-p)*(x-p))/(w*w))
- Line
y = c*y + a
APPROX: It calculates the theoretical shape of each selected peak. It permits to correctly ini-
tialize the peak fitting parameters
LabSpec Software 32
Icon 29 Integral calculation
Deviation factor
The optimization of the fitting parameters is done iteratively with the method of maximum gra-
dient. In each step (j) the deviation of the interpolation from the real spectrum is calculated
summing over all spectral pixels from f1 to f2.
d(j) = (If-Sf)*(If-Sf)
To calculate an integral under a band, zoom the band of interest. Select the green cursor, posi-
tion the first and the second cursor with the mouse. Then press the icon
It allows you to choose the colors for drawing of plane and 3D representations
of images.
LabSpec Software 33
Icons list
- TRUE:
Select a 3 (red, green, blue) colors palette, each color with intensity levels.
This drawing style is only used when superimposing two images.
- OTHERS
Palettes are the same as grey but the black and the white are substituted by other fundamental
colors.
You can also adjust the contrast and the brightness of your image
Icon 31 Zoom
Zoom in either X, Y, wave number and intensity dimensions. For images and spectra.
Extract
If you are just interested by a part of a spectrum or if you want to limit the wave number
domain of a spectral image, you can use this button:
First, you have to select the part of the spectrum (or the spectral image into all spectra
window ), for that you have to make a zoom by using the icon .
Then press the button Extr : on the screen you will have the selected part
.
LabSpec Software 34
Icon 31 Zoom
This window describes the algorithms which are used for the mapping. It gives us some spec-
tral information:
- Green/blue
It is the ratio between the contents of green and blue cursor of the window
all spectra, either in mode Raman and Fluo.
-Decomposition
It is the procedure used to make some spectra models (this option is in mathematical
treatments )
In this procedure, each spectrum of a spectral image is fitted with a sum of
model spectra.
The relative importance of each model in the fitting can be mapped.
-Extract procedure
Deconvolution
It gives you the percentage for the contribution of each of the model components in the dif-
ferent points of the mapping.
Summa
It gives you the error made by the calculation of the% of the different model components.
Raman/Fluo
LabSpec Software 35
Icons list
Icon 33 Model
1. Choose the spectra that will become MODELS : they can be already saved on the
disk, or they can be particular spectra that you can extract and save from your spectral
image.
2. If the spectrum is a particular one extracted from the image, save it.
4. Load all the selected spectra and put them overlapped in a window (for that use the
option behaviour of the Format menu command).
5. Activate the first selected spectrum and press the button GET. Do the same for all the
selected spectra: a window is then created with all the model spectra .
You will see in the mapping window additional pictures that correspond to each one of the
model .
You can overlapped them to see the relative contribution of each of the spectra in particular
point of the image (for that use the option behaviour of the Format menu command).
7. You can save a whole spectral image with the model compounds choosing the tvf
format. When you will load this image, the decomposition will automatically appear.
Video image grabbing. The video image is frozen by pressing the icon STOP (n46). The
type of cursor (buttons at the right lower edge of the window) indicates the type of object you
choose to record in imaging mode.
If you want to maximize the signal and adjust experimental conditions you can use this icon
This option is a spectrum adjustment (continuous recording of spectra, the new spectrum
refreshes the old one and so on), so it helps you in maximizing the signal and in adjusting
experimental conditions.
LabSpec Software 36
Icon 37 CCD image adjustment
This option permits to start spectrum recording without moving table in the point indicated by
the point into the video image. It is the simplest procedure to record a spectrum.
With this icon you can also do a multi window recording of spectra by choosing the parameters
in the window Spectral windows parameters (icon 21)
The procedure is the same as the one for adjusting a spectrum (refer to the help
of the icon ). But to record the CCD image you have to press the icon .
This option is useful to check the position of the spectrum on the chip in order
to select the good binning : You can see precisely the spectral domain of interest.
If you have an XY motorized table, or if you want to do a time kinetic recording, you can select
some points on the video image to have their spectra.
This procedure is used for recording spectral image or line scanning recording, time kinetics
and also spectra positioning the XY table in the video image, instead of the icon that
records the spectra without moving table.
To activate this icon you first have to record a video image, and you have to choose the type of
cursor that will define the area to analyze.
Meaning of these different cursors:
- Point cursor : You select or delete a point with the right button of the mouse,
you move a point with the left button of the mouse
- Inclined line : N spectra are recorded point by point along the line.
- Horizontal line : N spectra are recorded with Laser scanning along the line or
point by point.
- Elliptic and polygon : You can use these cursors if you are just interesting by a part of
the sample. It is a point by point recording.
LabSpec Software 37
Icons list
Three cursors are available and are selected with the buttons at the right lower corner:
- A red single cursor for the wavelength and the intensity readout.
- A double green cursor for the readout of the width of a band.
- A yellow cursor composed of a continuous line and two dotted lines. The continuous line
automatically positions to the maximum of the band in the neighborhood of the mouse arrow
and the two dotted lines position at the half maximum of the band.
This is the full representation of a spectral image. All the spectra that compose the spectral
image are displayed overlapped. In this window, three cursors allow the selection of the bands
for the selective spectral mapping.
This window contains the mapping of some spectral features. For example: mapping of a band,
ratio between two bands, spectral models decomposition etc...
This option permits to select the parameters of spectral images (Xsize, Ysize, Zsize, number of
spectral points). If you want to do a time Kinetic recording, the choice of the time interval is
done in this window.
In the box size, you can select the number of points (spectra) that you want to record along
each line. It is the binning (cf. Spectral) in spatial dimension (the short one).
Or if you want you can choose the step between two measurements in the box Step&Binning.
LabSpec Software 38
Icon 39 Data size
In this box you can select the number of lines of a spectral image.
Spectral.
This box contains the number of spectral points read out of the detector.
The number of data points along the wave number direction can be less than the number of pix-
els because you can choose to readout some pixels together. This technique takes the name of
binning. If two pixels are binned together, their intensities are summed up, thus you gain
in S/N, but, of course, you loose spectral resolution.
The binning of the pixels of the CCD can be useful if you are just interesting in mapping
the intensity of one spectral band.
For example, if you have a detector with 1024 pixels along the spectral direction, you can
choose to read all the pixels and then the spectrum will be composed of 1024 points.
If you don't need spectral resolution, you can choose to work, for example, with 200 data
points: the signal from 5 pixels is summed up and gives rise to only one data point. Of course,
1024/200 gives not an integer result and some pixels of the CCD will not be displayed.
Time
There are two types of boxes, the first one is the number of measurements required and the sec-
ond one is the time interval between them.
Be careful, because the acquisition time is included in the interval of time between two mea-
surements.
As for the time dimension there are two boxes, in the first one you introduce the number of
measures and in the second box you introduce the interval between the measures.
This option can be automatic with a piezo, if you have no piezo, the program stops, asks you to
move the focus and start again.
LabSpec Software 39
Icons list
Time
There are two types of boxes, In the first one you select the beginning and the end of the analy-
sis. In the second one you choose the time interval between them.
The choice of the time interval between the measurements is introduced as a function
of the number of the measure t .
A value: 30 * t will produce a spectrum each 30 seconds, 60 * t each minute.
You can introduce more complex timing like log (a*t), exp (a*t) or a*t*t where a is a con-
stant.
Depth
As for the time dimension there are two boxes, in the first one you introduce the beginning
and the end of the analysis and in the second box you introduce the interval between the mea-
sures which is a function of the number of the measure z.
This option can be automatic with a piezo, if you have no piezo, the program stops, asks you to
move the focus and start again.
You introduce the beginning and the end of the analysis. And you can choose to have a con-
stant size or a constant step between the measurements
You introduce the beginning and the end of the analysis. And you can choose to have a con-
stant size or a constant step between the measurements
LabSpec Software 40
Icon 39 Data size
Spike removing:
This option is active only when there is more than one accumulation; it is recommended to give
3 or more accumulations to have a good spike removing.
X scanning:
- Cursor:
The size of the image is selected with the cursor in the video image.
- Manual:
The upper left corner and the right bottom corner are selected moving the XY table.
If you choose this option, the software will ask you to move the table in the appropriate posi-
tion and validate.
LabSpec Software 41
Icons list
Refresh time
The PC can not record and display the data of a spectral image at the same time.
It must stop the recording in order to display the collected data, you can choose to stop period-
ically the recording and display the data by selecting a refresh time.
For the images, to reduce the total time of acquisition procedure, it is better to choose a refresh
time equal or higher than 10 minutes.
For example, if the refresh time is 300, it means that each 300 seconds, the recording of
a spectral image will stop, the display of data is refreshed on the screen, and the recording start
again.
Confocal value
- The intensity in function of the position of the point, if you have chosen confocal value =
Intens.
or
- The z value where the intensity of the signal is maximum, if you have chosen confocal value
= Depth
This option permits to select the stripe of the CCD used for single spectrum readout, or the
region of the CCD used in image mode.
The choice of the region of CCD used for measure allows you to limit the spectral domain of
interest and to avoid to read regions without signal that would only increase the readout noise.
Usually you have not to change these parameters (settled at DILOR) unless you use the fiber
optic entrance. In this case the interesting detector area will be substantially larger than the one
obtained under microscope.
LabSpec Software 42
Icon 42 Morphological images
When you record an image of the CCD, you can see the area where the bands are. So, with
these different parameters, you can select this area. You are allowed to limit the wave number
domain and the spatial domain.
Clicking on this icon will display a morphological parameters window. These parameters will
allow you to get morphological images.
To have access to this feature, you have to take a Video image and choose the rectangular cur-
sor in this image.
The three first lines allows you to take morphological images in function of the depth.
In the First Z and Last Z fields, enter the beginning and the end of your depth profile (e.g.:
-10 and 10).
In the field Size Z, enter the step between two measurements in depth.
Size X and Size Y are the number of points you want to analyze in the X and Y direction.
Intensity is the number of counts read out from the PMT during the time defined in the
Time field.
Repeat is the number of total measurement cycles.
Single image option allows you to save all of the morphological images depth profile on a
file.
To start measurement, press on the START button.
Icon 43 Calibration
This window is used to calibrate the moving of the XY table and the amplitude of the scanner.
1. First, you have to select the appropriate objective and record a video image of the sample
with the laser spot. This one must be attenuated in order to see its position well. The sam-
ple should have some sharp features (corners, dots, dust particles etc...).
2. Press the button .
3. Choose the cursor point into the video image, then position the small rectangle on
the laser spot. Press SET on the box Centr (x,y) of the Scale window . The
calibration of the laser is done, and when you will record a new video image, a color dot
LabSpec Software 43
Icons list
6. Position the two corners of the rectangular cursor onto the selected details of the sample,
then introduce in the box Table (dx,dy) the dimensions of the rectangle as measured
with the XY table. Press SET on this box.
The equivalence between the video camera pixels and the micrometers are now settled.
You can check the calibration of the table if you record a spectrum in a selected point of
the video image.
- Select the horizontal line cursor on the video image with the length approximately 10% less
than the scanned line and center it on the laser spot.
- Open the window SCALE . Press SET on the box Scanner (l,r) .
You can check the calibration of the scanner doing an image of the sample.
If the calibration is not correct, select the cursor line shorter or longer and press again SET
on the box Scanner (l,r) .
LabSpec Software 44
Icon 43 Calibration
Icon 44 Configuration
To use one of your programmed configurations, in the window configuration select the
desired configuration then press set , the software will automatically change all the parame-
ters.
LabSpec Software 45
Icons list
Icon 45 Scheme
This icon permits you to control all the functions of your system:
control: change:
Acquisition
Spectrum time
Different
Acquisition
cameras (A)
Spectrograph
Laser shutter position
(B)
Image Grating
Filters wheel
(C)
Acquisition
C B
Hole aperture
White lights
A
Exciting line
(D)
D
Different secu-
rity switches
(E)
D E
Icon 46 Stop
Stop of spectra recording. Freeze the video image after pressing the Video button (icon n34)
Three cursors are available and are selected with the buttons at the right lower corner:
- A red single cursor for the wavelength and the intensity readout.
- A double green cursor for the readout of the width of a band.
- A yellow cursor composed of a continuous line and two dotted lines. The
continuous line automatically positions to the maximum of the band in the
neighborhood of the mouse arrow and the two dotted lines position at the half
maximum of the band.
LabSpec Software 46
Icon 47 Different types of cursors
To print a spectrum, select the spectrum to print (it must be alone in the window, if not it will
print all the object of the active window) then to insert it in your print page press the icon .
To introduce the parameters in your print page, press the icon , select the parameters and
then press OK.
LabSpec Software 47
Icons list
Copy Text: Copy the text or the parameters that have been selected in the print page.
Copy picture: Copy the contents of the active window
Copy data: Copy the data of the active object
Paste Text: Allows the ability to paste text in the print page
Paste picture: Allows the ability to paste a picture in the print page
Paste Data: Allows the ability to paste a spectrum in the Spectrum window
This function can be useful to keep an object before correction opera-
tions, if these modifications are not used we just have to recall the origi-
nal object with this option.
VIEW
DISPLAY:
BEHAVIOUR
LabSpec Software 48
Icon 47 Different types of cursors
SUPERPOSITION
: Only limited spectra are shown (min. and max.) with filled space between them.
: All the spectra are overlapped in the window (it could take long time when the PC dis-
plays the collected data)
: Only a part of the spectra is shown to make displaying time less than 1-3 seconds.
: 25% of the spectra are shown.
: Unsmoothed display.
: Smooth along lines.
: Smooth along columns.
: Smooth.
IMPOSITION
These icons correspond to the different forms of overlapping the recorded spectra or spectral
images on the video images.
a : Off mode.
b : It gives you the points where spectra were recorded
on the video image.
c : It is the same as b but it also displays the names of
the spectra.
d : It gives the rectangle where spectral images are
recorded.
e : The same as d but it also gives the names.
f : It gives you the superimposition of spectral images
on the video image.
COLORS
You are allowed to put or eliminate a coordinate axis (wave number, intensity, X or Y) or put a
label on an axis (for example: wavenumber (cm-1): select with an X the axes or labels that
you need.
The expressions for the labels can be changed
You can also change the color of a spectra or of the background
LabSpec Software 49
Icons list
3D MODE:
SCALE
LabSpec Software 50
Icon 47 Different types of cursors
Full list of the objects contained in a window (for example, list of the spectra
contained in a spectral window).
Multi: This procedure selects all the objects that are on the screen and permits to
make an operation to all these objects. For example, you have three spectra on
your window, and you want to add the same constant to all these spectra,
activate the option multi , the addition will be done on the three spectra. If the
option multi is not active, you will have to do this operation for each
spectrum.
LabSpec Software 51
Icons list
Auto save:
1.1 You can choose the directory where the spectra will be recorded:
1.2 You can choose the files where the spectra will be recorded:
LabSpec Software 52
Icon 47 Different types of cursors
2.1 You can choose to work with the Auto Save and Auto Repeat options:
For that you choose the delay between two acquisitions (ex: 10 seconds). When you press the
icon the software takes an acquisition and record it each 10 seconds.
2.2 You can choose to work only with the Auto Save option:
- When you press the icon the software takes an acquisition, stop and record it.
- When you press the icon (that you use to do spectral imaging, time scanning...) the soft-
ware take a spectral image and at the end of the acquisition will record it.
Reorganize: This option permits to re-organize the windows. And permits to select
one of the windows that are on the screen (of course, you can select a
window with the mouse).
LabSpec Software 53
Icons list
LabSpec Software 54
Icon 47 Different types of cursors
LabSpec Software 55
Icons list
2. Select the correct grating setting in the software. If you change the grating, do not forget
to go to the zero order position ( in the software). This will give the correct calibration
for the particular grating you will use.
3. Focus the laser on the sample. This can be done through the video image .
6. Select the acquisition time and the number of accumulations (this will improve the sig-
nal/noise ratio)
- The icon : is a spectrum adjustment, so it can help you in maximizing the signal (the new
spectrum refreshes the old one and so on...). No repeated accumulations or extended spectral
ranges are acquired.
If you dont save the spectrum that you have recording with the icon , it will be
automatically deleted as soon as you will record a new spectrum.
- If you press the icon it will make a spectrum accumulation and stop.
LabSpec Software 56
Icon 47 Different types of cursors
For LabRam and LabRam Infinity Systems configured with the motorized
XY Stage
You can select different points to analyze on the sample, for that, choose the cursor point
and position a small rectangle on each point that you want to analyze with the right button of
the mouse. If you want to move the point, drag the box by holding down the left mouse button,
if you want to delete a point, press the right button.
Select between 1800 and 600, 300 or 1200 grating options. If you change the grating do not
forget to make a zero order.
6. Select the hole aperture and the slit apertures (for the LabRam System). Select the time
of exposure and the number of accumulations Accum .
LabSpec Software 57
Icons list
Confocal mapping
For LabRam and LabRam Infinity Systems configured with the motorized
XY Stage
5. Select the hole aperture and the slit apertures (for the LabRam System) and the time
of exposure.
LabSpec Software 58
Icon 47 Different types of cursors
In the LabRam:
- The stem 2 is pushed.
- Replace the pinhole H1 by the field lens.
On the software:
6. Select the slit and the hole apertures and the exposure time.
NOTE: Do not forget to switch off the scanner, and replace the pinhole H1 once you have fin-
ished your linescan measurements.
LabSpec Software 59
Icons list
This option enables you to take a series of spectra or spectral images as a function of time.
You follow the same procedure as the one to record point by point spectra or spectral images.
Be careful, because the acquisition time is included in the interval of time between two mea-
sures.
LabSpec Software 60
Icon 47 Different types of cursors
Z scanning
Tip6: Z scanning
6. In the window Data size , you select the parameters of the Z scanning.
Please refer to the Index section
LabSpec Software 61
Icons list
Baseline correction
The main procedure is to build up point by point a baseline, to fit at best with the spectrum and
then subtract.
1. Verify that the option Ins/Del is active (in the box operations )
2. Select the type for interpolation: it can be linear or polynomial (in the box type ). For
the polynomial interpolation, you can also choose the degree of the polynom (in the box
degree ).
3. You can select with the mouse in the spectrum window (validate: left button, delete: right
button) the points for the baseline computation
Tip: (for the images, when you press the icon , a window that contains an average spec-
trum of the image is opened automatically).
4. Activate the option attachment , to fit at best the baseline to the spectrum.
Tip: An automatic procedure can also be used for the computation of baseline: button
Auto
NB: The baseline can be saved as a spectrum: for that press the button CONV
LabSpec Software 62
Icon 47 Different types of cursors
Peak Fitting
To obtain a good Gaussian/Lorentzian fitting, you should prepare the spectrum from spikes.
1. Zoom and extract (button extract of the icon ) the interesting part of the spectrum
(or of the spectral image).
3. Select the function (button FUNC ), you can choose particular function for each band
of the spectrum.
For the images, the procedure is the same as the one for the spectra, but the selection of max-
ima is done in the window Spectral image and you have to give a long time for the proce-
dure of peak fitting (more than 5 minutes).
The results of the peak fitting procedure are in the window Bands . You can save these
results when you press the button save (the files format is *.bnd).
If you want to print the board that contains the results of the peak fitting, open the window
Bands Parameters , do a copy text, open the window Print page
(option page setup of the edit menu) and to insert this board, choose the option paste
text of the main menu.
Moreover, for the images, you can have the mapping of the position, the surface, the amplitude,
the width at half maximum of the band: In the window Band parameters press the buttons p
(for the position), a (for the amplitude), w, s or g the results are in the window Spectral
mapping .
LabSpec Software 63
Icons list
1333.3
1000
10
800
20
600
30 400
200
40
Video image
10 10
20 20
30
30
40
40
0 10 20 30 40
0 10 20 30 40
Length X (m)
L th X ( )
Mapping of the position of the band Mapping of the full width at half
White color corresponds to the maximum. White color corresponds
1336 position, the black one to the to a 9 cm-1 FWHM and the black
1332 cm-1 position one to a 4 cm-1 FWHM
10
20
Overlapped of the FWHM and posi-
tion mapping. The shift of the band
30 goes with an enlarging of this band
40
0 10 20 30 40
Length X (m)
LabSpec Software 64
Icon 47 Different types of cursors
Models
Tip9: Models
1. Choose the spectra that will become MODELS , they can be already saved on the
disk, or they can be particular spectra that you can extract and save from your spectral
image.
2. If the spectrum is a particular one extracted from the image, save it.
4. Load all the selected spectra and put them overlapped in a window (for that use the
option Behavior of the menu view of the FORMAT menu).
5. Activate the first selected spectrum and press the button get. Do the same for all the
selected spectra: a window is then created with all the model spectra .
You will see in the mapping window additional pictures that correspond to each one of the
model .
You can overlapped them to see the relative contribution of each of the spectra in a particular
point of the image (for that use the option Behavior of the menu view of the FORMAT
menu
7. You can save a whole spectral image with the model compounds choosing the tvf
format. When you will load this image, the decomposition will automatically appear.
LabSpec Software 65
Icons list
20
40
The spectra have been taken
60 inside the rectangle.
80
100
With the help of the mapping, we can distinguish three different spectra which are:
0.6
0.6
0.4
0.4
0.2
0.2
0.0
0.0
800 900 1000 1100 1200 1300 1400 800 900 1000 1100 1200 1300 1400
0.8
0.6
Blue compound
0.4
0.2
0.0
We can have the mapping of the relative contribution of each compounds in the analyzed
sample:
Red compound
Blue compound
20
Length Y (m)
40
Green
60 compound
80
20 30 40 50 60 70
Length X (m)
LabSpec Software 66
1.6 LabSpec for the T64000 System
This chapter will only detail the LabSpec specific features related to the T64000 System.
LabSpec Software 67
T64000 Configuration Setup
The figure above shows the icon which allows the user to setup the T64000 configuration. The
following screen will then be displayed.
First Stage Entrance The default choice is Axial especially if the Microscope or the Macro-
sample compartment is used. The Lateral choice is an option for spe-
cific needs required by the user.
Analysis mode The Micro mode concerns the use of the Microanalysis System, the
Macro mode concerns the use of the Macroanalysis System. Both are
options. the selected option must be present on the system.
LabSpec Software 68
Spectrograph The T64000 is a triple stage spectrometer. Therefore, the last stage can
be used alone (Single) for some specific applications.
Premonochromator The Premonochromator includes the first two stages. By default these
stages are configured as subtractive. An additive option can be added.
See the hardware user manual for details.
Second/Third Stage The link on the exit of the premonochromator and the entrance of the
third stage can be direct (default) or external (option). That means that
the exit beam can be adapted for a specific application (e.g. Fluores-
cence option) then analyzed with the third stage.
Detection System Select the detector you want to use: Multichannel (CCD) or
Monochannel (Single channel PMT or IR detectors).
Laser Shutter The laser shutter is located before the Microanalysis or Macroanalysis
System. Usually, to prepare an analysis session, the laser beam must be
present in the microscope or in the macro-sample compartment. With
some type of samples, the laser beam can modify or destroy a sample.
For these reasons, two choices are available: Always open maintains
the shutter off and Open during acquisition opens this shutter only dur-
ing the acquisition (adapted for laser sensitive samples).
Initialization Clicking on this button will reinitialize all the commutations listed in
this screen plus the grating turret.
LabSpec Software 69
Monochannel/Multichannel Setup
The previous chapter explains how to select the Monochannel or Multichannel detector for the
System which includes both of these detectors.
Default, on all T64000 Systems, the Monochannel (PMT) detector is mounted on the third
stage axial exit and the Multichannel (CCD) detector is mounted on the top exit. Once the Lab-
Spec software is running, the bottom left screen shows the following setup screen, specifically
dedicated to the T64000 System. The use of the displayed parameters is slightly different
depending on the chosen mode: Monochannel or Multichannel.
LabSpec Software 70
How to setup a Monochannel PMT (PhotoMultiplier Tube) detector?
On the figure page 70, the bottom left bar includes two new sections fitted for the T64000 Sys-
tem: the Premono(chromator) and Spectr(ometer) sections.
From these sections, the following setup can be performed:
Single or Double moving: Double moving means that the Premonochromator and the Spec-
trometer scan together. Single moving means that the Premono-
chromator is independent from the Spectrometer.
Calibration screen: This feature allows the user to precisely calibrate the premono-
chromator and the Spectrometer. The complete calibration pro-
cedure is described in the Monochannel Mode Calibration
chapter.
Monochannel Security Value
Enter a value which defines the safety area from the laser beam.
LabSpec Software 71
The Disable choice is only for those who want to manually
explore the near-to-laser area and in this case TAKE CARE
NOT TO DESTROY THE CCD DETECTOR.
Once the LabSpec software is running with the Multichannel option selected (see previous
page), select the Multichannel detector setup screen by clicking on the following icon:
LabSpec Software 72
CCD Detector Setup
This option allows the user to select the stripe of the used CCD for single spectrum readout, or
the window of the CCD used in image mode.
The choice of the region of the CCD used for measure allows you to limit the spectral domain
of interest and to avoid reading regions without signal that would only increase the readout
and dark noises.
LabSpec Software 73
This following figure shows an example of a 256 x 1024 detector).
When you acquire a CCD image, the useful area will be clearly seen. So, with the parameters
of the Detector setup, you can select this area. You are allowed to limit the spectral domain and
the spatial domain.
LabSpec Software 74
Monochannel Mode Calibration
The following procedures will explain how to calibrate the System using a monochannel (PMT
or IR) detector.
Usually, the T64000 System and the LabSpec software have been installed and started up by
Dilor-Jobin Yvon-Spex or an approved representative. Then, the first time calibration has
been already performed.
Calibration troubleshooting
The troubleshooting calibration can occur if, during a scanning, the power supply shuts down
or if a link fails somewhere. The Premonochromator and Spectrometer position fields will
show ??? . Click on the calibration button of the Spectrometer and enter the
Counter Value.
Preliminary
The T64000 System can be perfectly calibrated using a known peak. This adjustment can be
done in accordance with the following limitations:
1. The calibration will be perfectly reached on the wavelength or wavenumber peak used for
the correction. For this reason, try to choose a calibration peak near to your working
region.
2. The resulting calibration offset will be saved (if close to the counter wavelength or wave-
number value). Each grating has its own value but NOT if you change the detector, for
example the Multichannel. In other words, if you change the detector or choose the Mul-
tichannel, verify and eventually create a new calibration offset (the procedure is
explained below).
Procedure
2. From the Commutations screen (see page 68) select the Monochannel Detection System,
LabSpec Software 75
4. On the Monochannel dedicated icon bar, click on the RTD icon. The RTD will start.
Running Acquisition
LabSpec Software 76
5. Monochannel Parameters: The Monochannel parameters allows the user to
prpare the acquisition parameters. Follow the instructions describe on the
screen below:
Enter the
Select the PMT high
hardware voltage wor-
acquisition king value
gain (1,10,100)
of the channel 1 Enter the
(if the channel is hardware
valid) acquisition
gain of the
channel 2
(if valid)
LabSpec Software 77
6. Real Time Display: During the RTD acquisition, the Monochannel icon bar is replaced
by the RTD Control Panel (see below)
Current position
Continuous scanning Stop RTD mode
Step by step scanning Start Time
7. Using the RTD Control Panel shown above, scan the spectrometer to the chosen refer-
ence peak. The current cursor is represented as a vertical red line located on the screen.
When the reference peak is founded, stop the scanning (icon located on the upper right
screen), choose the step by step scanning mode and click on the reverse direction scan-
ning up to the highest intensity level of the reference peak (the intensity level is dis-
played on the right bottom bar of the screen). Stop the scanning at this position. Then do
not change the position and follow the next step.
The figure located on the next page (page 80) shows the general LabSpec monochannel
screen during the reference peak search step.
8. Click on the close button of the RTD Control Panel, then click on the Close button of the
Monochannel icon bar.
9. Running Acquisition: Once the Monochannel Parameters have been correctly setup
and once confirmation has been performed with the Real Time Display feature, you can
run the acquisition.
LabSpec Software 78
10. Click on the Spectrometer Calibration button to display the calibration screen then:.
C. Click OK to validate
The spectrometer is then calibrated
LabSpec Software 79
Stop RTD
LabSpec Software
Cursor line
Current Intensity
level
80
Multichannel Mode Calibration
The following procedures will explain how to calibrate the System using a multichannel
(CCD) detector.
Usually, the T64000 System and the LabSpec software have been installed and started up by
Dilor-Jobin Yvon-Spex or an approved representative. Then, the first time calibration has
been already performed.
Calibration troubleshooting
The troubleshooting calibration can occur if, during a scanning, the power supply shuts down
or if a link fails somewhere. The Premonochromator and Spectrometer wavelength or wave-
number fields will show ??? . Click on the calibration button of the Spectrometer
and enter the Counter Value.
Preliminary
The T64000 System can be perfectly calibrated using a known peak. This adjustment can be
done in accordance with the following limitations:
1. The calibration will be perfectly reached on the wavelength peak used for the correction.
For this reason, try to choose a calibration peak near to your working region.
2. The resulting calibration offset will be saved (if close to the counter wavelength or wave-
number value). Each grating has its own value but NOT if you change the detector, for
example the Monochannel. In other word, if you change the detector or choose the
Monochannel, verify and eventually create a new calibration offset (the procedure is
explained below).
Procedure
2. From the Commutations screen (see page 68) select the Multichannel Detection System,
3. Click on the Multichannel calibration icon to start the calibration mode . The fol-
lowing screen will be displayed:
LabSpec Software 81
Reference peak
Central pixel
Spectrometer position
The page 73 shows how to setup the CCD matrix. On the screen above, the System performs a
Real Time acquisition with this additional important parameter: the central pixel of the CCD
is permanently displayed. To calibrate the spectrometer, follow the procedure:
1. Choose a known reference peak and enter the reference position (see above),
2. If the spectrometer is not too de-calibrated, you should be able to see on the screen this
reference peak.
3. Try, by entering a new position value, to center the reference peak on the central pixel.
This could be performed by successive estimations. Zooming the central area will
increase the calibration setting.
4. Once the reference peak is perfectly set on the central pixel, click on the Calibration but-
ton of the Spectrometer panel. Then follow the procedure described below:
LabSpec Software 82
A. Select the Real Position choice
C. Click OK to validate
The spectrometer is then calibrated
The T64000 System is composed of three stages: the first two stages are called Premonochro-
mator, the last stage is the Spectrometer.
By default, the Premonochromator stages are configured as a subtractive optical design. In this
case, the Premonochromator performs a filtering function. If the highest results are required, a
Premonochromator calibration is necessary to perform a band-pass on a working area. The
procedure described below will show how to optimize a calibration.
The goal of this procedure is to close as much as possible the intermediate slit while the refer-
ence peak stays visible on the center of the CCD detector.
1. Calibrate the Spectrometer using the procedure described on page 81; at the end of this
procedure, the reference peak is displayed at the center of the CCD detector,
2. From the Security and Optimization screen (see page 70), choose Disable the Premono-
chromator and First Slit optimization,
3. From the Premonochromator Control Panel click on the Single Moving choice,
4. On the same Control Panel, click on the following button to display the slits list
and select the First Intermediate Slit.
5. Click on the CCD Calibration icon to run the calibration Real Time acquisition,
6. In the slit width field, enter a smaller value than displayed (about 10% less) and verify
that the peak is always visible and centered on the CCD. Continue to reduce the interme-
LabSpec Software 83
diate slit. If the spectrum disappears, that mean that the position (Premonochromator) is
not centered on the beam path. To re-center the filter, enter a new position value in the
Premonochromator field, more or less you have to try, or slightly open the intermediate
slit to re-display the spectrum. This is a step by step procedure, and once reached the
minimum slit width value, you know that the premonochromator is optimally adjusted
and the Acquisition session can be now performed.
1 2 3 4 5 6
7 8 9 10 11
1. Available slits selection list
2. Current slit calibration
3. Single moving (Premonochromator and spectrome-
ter are independent) or double moving mode switch.
4. Premonochromator calibration
5. Security and Optimization modes
6. Spectrometer calibration
7. Close the current slit
8. Slit width field
9. Open the current slit
10. Premonochromator position field
11. Spectrometer wavelength field
LabSpec Software 84
Interac-
tive Index Symbols Deconvolution 35
*Const 20 Definition of the function 18
+Const 20 Delete the object in the window 14
Depth 40
Numerics Description of the different icons 13
3D MODE 50 detection of the peak 30
detector 39
A Detector parameters 42
Acquisition panel 12 Detector setup (T64000) 74
Acquisition parameters 41 Deviation 21
Add a spectrum 25 Deviation factor 33
additive 69 DFT 29
All spectra window 38 Dilor format 15
Analysis mode 68 Double moving 71
Apodisation 29
APPROX 32 E
Attachment 21 Edit, Copy data 48
Auto Repeat 53 Edit, Copy picture 48
Auto save option 52 Edit, Copy Text 48
Autofocus 42 Edit, Paste Data 48
Edit, Paste picture 48
B Edit, Paste Text 48
Baseline correction 19 Exchange a spectrum 24
Baseline correction example 62 Exit LabSpec 47
binning 39, 57, 61 Extended Tiff 15, 16
Extract procedure 35
Extract profile 25
C Extracted spectrum 34
Calibration (spectrometer,
monochannel) 79
Calibration (XY stage) 43 F
CCD detector calibration FALSE 33
(T64000) 82 File, Delete 47
CCD image adjustment 37 File, open 47
CCD Matrix setup (T64000) 73 File, Print 47
Color Tiff 16 File, Save 47
Commutations screen 75 File, Split 47
Compressed Tiff 15, 16 Filter 12
Configuration (system) 45 Filtering 26
confocal hole 56 Fit peak width 14
Confocal mapping 58 Fitting (peak) 63
Confocal value 42 Focusing the laser 57
Constant for addition 18 Format, BEHAVIOUR 48
Constant for multiplication 18 Format, COLORS 49
Correction 21 Format, IMPOSITION 49
cursor for working 13 Format, SUPERPOSITION 49
Cursor normalization 16 Format, VIEW 48
cursors (Red, Green, Blue) 35 Formats (Image) 16
formats for spectra saving 15
Fourier transform (filtering) 29
D
Data size 38
Decomposition 35
H N
Hole 12 Normalization 16, 22
hole aperture 12 number of accumulations 12
Horizontal shift 13
How to acquire a spectrum 56 O
How to record spectra at selected object 51
points? 57 offset (autofocus) 42
Operations between two spectra 18
I Operations for the baseline 20
IFT 29 Option menu commands 51
Information about object 16
Initialization 69 P
Ins/Del 20 Palette window 33
Insert a spectrum 23 peak detection 30
Integral calculation 33 Peak elimination 13
Intensity adjustment 13 Peak fitting 30, 63
inverse Fourier transform 29 PeakElm 27
Polynomial filtering 28
K Position of the spectrograph 12
Kinetic 60 Premonochromator calibration
(T64000) 83
L Premonochromator calibration and
Label of the peak 14 optimization 83
LabRam 57, 58, 59 Print page 16
LabRam Infinity 57, 58 printer page configuration 47
Laser 12 Printer set-up 47
laser 56 Profile 23
Laser Shutter 69
Line scan illumination 59 Q
Load 14 Quit LabSpec 47
Lorentzian 32
R
M Raman/Fluo 35
Manual addition 13 Recording time 12
Mathematical filtering 29 Refresh time 42
Mathematical processing 17 Removing spectrum 23
Menu Bar Description 47 Reorganize 53
Model 36 RTD acquisition (T64000) 78
model 65
Models 65 S
Monochannel Mode save a TV image 15
Calibration 75 save spectra 15
Monochannel/Multichannel Save the active object 14
Setup 70 scale of a spectrum 13
T
T64000 Configuration Setup 68
T64000 Macro-sample 68
T64000 Microscope 68
T64000 Premonochromator 69
T64000 spectrometer 69
T64000 System 67
Text format 15, 16
thr(%) 21
Threshold 21
TIME 32
Time 12, 39, 40
Time kinetic recording 60
TRUE 34
TSF format 53
types of cursors 46
U
units 51