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# PAPER 3 BIOLOGY ANSWERING TECHNIQUES rate of enzyme

## (amylase) reaction is low

PAPER 3: 2 QUESTIONS 1. 33 MARKS / decreases / longer /
2. 17 MARKS lower.
TOTAL: 50 MARKS
QUESTION 1: Vertical inference:
1) How to state the observation? At temperature 37C,
the rate of enzyme
- Must always involve 2 CRITERIAS: reaction is higher than
- EX: Time the rate of enzyme
Temperature reaction at 5C / 28C /
Incomplete average answer Accurate answer (3 marks) 45C / 55C // vice
(1mark) versa.
Observation 1: Horizontal observation:
The time is different for At temperature 5C, time
each temperature. taken for iodine solution 3) How to get full marks in hypothesis for an experiment?
remains yellowish is 12
Observation 2: minutes. - HYPOTHESIS = MV + RV
The temperature influences Vertical observation: - Manipulated variable: temperature
the time taken for iodine to Time taken for Responding variable : time taken for iodine solution remain
remain yellowish. iodine solution remain yellowish / rate of enzyme (amylase) reaction / activity
yellowish for Incomplete average answer Accurate answer (3 marks)
temperature 37C is (1mark)
faster than 5C / 28C / The temperatures The higher the
45C / 55C influence the enzyme reaction temperature, the
higher the rate of
2) How to state inference in Paper 3 Biology? enzyme reaction / time
taken for iodine solution
- Also involves 2 criterias: remain yellowish (until
- EX: Rate of enzyme reaction it reaches the optimum
Low / High (Reject: Lowest / Highest) temperature)
Incomplete average answer Accurate answer (3 marks) When the temperature
(1mark) increases, the rate of
Temperature influences Horizontal inference enzyme reaction also
the enzyme reaction. At temperature 5C, the increase (until the
optimum temperature) starch was hydrolysed (completely) by
amylase at the maximum rate.
4) How to draw the graph correctly in Paper 3 Biology?

## SAL or SAP which is S = Shape, A = Axis and L = Label / P = Point.

Besides that, student must remember to write the title of the graph.

## 5) How to explain the relationship in Biology?

In Biology Paper 3, it is more specified as compared to Chemistry. Students
must provide the manipulated variable first and follow by responding 6) How to answer the last question (Question number 2)?
variable and usually for the manipulated variable must have two supporting There are 9 sub sections (total 17 marks) that students need to
theories and a brief conclusion. answer. The mark distribution is as followed:
1. Problem statement (3 marks)
EX: 2. Objective (1 mark)
Incomplete average Accurate answer (3 marks) 3. Variables (1 mark)
answer (1mark) 4. Hypothesis (3 marks)
The higher the Students must state the relationship 5. List of material and apparatus (3 marks)
temperature, the between the rate of enzyme reaction and 6. Technique used (1 mark)
higher the rate of the temperature base on criteria: 7. Experimental procedure or method (3 marks)
reaction R1 Relationship: the temperature 8. Presentation of data (1 mark)
increases, the rate of enzyme reaction also 9. Conclusion (1 mark)
increase. (Theory 1)
R2 Explanation 1: at 37C // at optimum 1) Problem statement (3 marks)
temperature the starch hydrolyse Problem statement must be in question form.
(completely by amylase / enzyme) (Theory i.e.
2) Does (MV) affect (RV) of xxx?
R3 Explanation 2: at the maximum rate How does (MV) affect the (RV) of xxx)?
(Brief conclusion) What is the effect of (MV) on the (RV)?
As temperature increases (MV), the
rate of enzyme reaction increases 2) Objective (1 mark)
(RV). At optimum temperature the It is a statement form:
i.e.
To determine the effect of (MV) on the (RV) of a xxx. 9) Conclusion (1 mark)
To study the effect of (MV) on the (RV) of a xxx Students are able to write a suitable conclusion for the
To investigate the effect of (MV) on the (RV) of a xxx experiment.
i.e.
3) Variables (1 mark) Ther higher/lower (MV), the higher/lower (RV). Hypothesis is
The entire variables (MV, RV and FV) must be correct to gain accepted/rejected.
one marks from here.

4) Hypothesis (3 marks)
The hypothesis must start with manipulated variable and follow
by responding variable (MV + RV)
5) List of material and apparatus (3 marks)
Students need to separate the list of material with the list of
apparatus.
i.e.
Apparatus: photometer, stopwatch, cutter, beaker, meter rule,
basin, marker/ thread
Material: Balsam plant, water, Vaseline/grease, dry cloth

## 6) Technique used (1 mark)

Students need to state the technique used in carrying out the
experiment correctly.
i.e.
Measure and record the time taken for the air bubble to move
a distance of 2 cm by using a stopwatch.

## 7) Experimental procedure or method (3 marks)

Students are able to mention all the MV, RV and FV in the
sentence.

## 8 ) Presentation of data (1 mark)

Students are able to draw a complete table and record the
relevant data base on 3 criteria.