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FEROZSONS LABORATORIES LIMITED

Standard Operating Procedure

SOP No. QC – 047-01
Effective Date:
Review Date:
Page No: 1 of 12

Procedure For Operation & Cleaning of Universal Incubator UN-260
Title Signature Date
Written by A.M. Microbiology

Reviewed & Quality Control Manager
Approved by
Reviewed & Sr. Manager Quality
Approved by Assurance
Approved by Director Quality Operations

1. OBJECTIVE
Objective of this SOP is to describe the procedure for operation of Universal Incubator
(Memmert).

2. SCOPE
This SOP describes the execution of operation of universal incubator in which
environmental conditions can be controlled for growing microbial cultures; this SOP is
applicable to microbiology laboratory.

3. GLOSSARY
Temp. = Temperature
SS = Stainless Steel
°C = Degree Centigrade
SOP = Standard Operating Procedure

Incubator = an apparatus in which environmental conditions such as temperature can be
controlled for growth of microbial cultures.

Temp. = the degree or intensity of heat present in a substance or object, especially as
expressed according to a comparative scale and shown by a thermometer or
perceived by touch.

SOP = is a set of step-by-step instructions compiled by an organization to help workers
carry out routine operations.

Reviewed & Approved by: Sr. MQA Sign: Date

All or part of the information contained in this document should be treated as the confidential property of Ferozsons Laboratories
Limited. It cannot be published or divulged for whatever purpose to unauthorized persons, or 3 rd parties, in any form without the prior
written permission from DQO Ferozsons Laboratories Limited.

COPY NO.

To provide adequate resources for the implementation of this SOP as defined. • Director Quality Operations: .To interface this SOP to the actual practices of flow patterns. • Quality Assurance Manager: . . . • A.To ensure that the SOP is updated & reflects the actual execution. • Microbiologist: .To keep the relevant SOPs valid and updated. °C = a unit of measurement of temperature on a specified scale also known as Degree Celsius. QC – 047-01 Effective Date: Review Date: Page No: 2 of 12 Procedure For Operation & Cleaning of Universal Incubator UN-260 SS = a form of steel containing chromium. . 4.To review the SOP in case of any change or whenever it is due for revision. or 3 rd parties. Reviewed & Approved by: Sr. resistant to tarnishing and rust. • Quality Control Manager: .To ensure that the SOP is followed and should be placed and user should be trained accordingly. Microbiology: .FEROZSONS LABORATORIES LIMITED Standard Operating Procedure SOP No.To assure through periodically audits that the SOP is valid and followed accordingly. RESPONSIBILITIES • General Manager: . COPY NO. M. MQA Sign: Date All or part of the information contained in this document should be treated as the confidential property of Ferozsons Laboratories Limited.To ensure that the SOP placed at the proper place and user should be trained accordingly. . It cannot be published or divulged for whatever purpose to unauthorized persons. in any form without the prior written permission from DQO Ferozsons Laboratories Limited.To follow the instructions laid down by this SOP.

Temperature Sensor 4. FEROZSONS LABORATORIES LIMITED Standard Operating Procedure SOP No.Stainless Steel Shelves 6. Model – IN-260 1 2 9 3 Universal Incubator 4 Components 1. or 3 rd parties. It cannot be published or divulged for whatever purpose to unauthorized persons. in any form without the prior written permission from DQO Ferozsons Laboratories Limited.On/Off Button 3.Door Handle 6 9.Ventilation Flap 8 5.Turn Controller with Confirmation Key 7 Figure – 1: IN-260 Universal Incubator Reviewed & Approved by: Sr.1 Universal Incubator. COPY NO. MQA Sign: Date All or part of the information contained in this document should be treated as the confidential property of Ferozsons Laboratories Limited. . REQUIREMENTS 5.Nameplate Covered 8. Memmert.Working Chamber 7. QC – 047-01 Effective Date: Review Date: Page No: 3 of 12 Procedure For Operation & Cleaning of Universal Incubator UN-260 5.Control COCKPIT with Capacitive 5 Function Keys 2.

3 Plug the provided power cable into the rear of the appliance and connect it. Figure – 3: Set the Temperature Reviewed & Approved by: Sr.FEROZSONS LABORATORIES LIMITED Standard Operating Procedure SOP No.4 Turn the power switch on by pressing the On/Off switch on the front of the sterilizer. QC – 047-01 Effective Date: Review Date: Page No: 4 of 12 Procedure For Operation & Cleaning of Universal Incubator UN-260 5.2. or 3 rd parties.2. so that. MQA Sign: Date All or part of the information contained in this document should be treated as the confidential property of Ferozsons Laboratories Limited. see figure – 2.5 First start-up display appears on the screen. 5.1 Ensure that the instrument is clean and free from dust and placed in a safe area.2 Lay the power cable. in any form without the prior written permission from DQO Ferozsons Laboratories Limited. figure – 3. 5.2.2 OPERATING PROCEDURE 5. . set the desired temperature by pressing the activation key on the left or right of the respective display. COPY NO.2. Figure – 2: Switch-on the Incubator 5. 5. It cannot be published or divulged for whatever purpose to unauthorized persons. it doesn’t come into contact with any hot part.2.

. It cannot be published or divulged for whatever purpose to unauthorized persons. figure – 4a & figure – 4b. adjust the set value (e.FEROZSONS LABORATORIES LIMITED Standard Operating Procedure SOP No.0 °C & 33.g.2. or 3 rd parties. MQA Sign: Date All or part of the information contained in this document should be treated as the confidential property of Ferozsons Laboratories Limited.0 °C). in any form without the prior written permission from DQO Ferozsons Laboratories Limited. QC – 047-01 Effective Date: Review Date: Page No: 5 of 12 Procedure For Operation & Cleaning of Universal Incubator UN-260 5.6 By turning the knob to the left or right. Figure – 4a: Turn the knob left or right Figure – 4b: Turn the knob left or right Reviewed & Approved by: Sr. . COPY NO. 23.

7 Save the set value by pressing the center button of the knob (confirmation key). MQA Sign: Date All or part of the information contained in this document should be treated as the confidential property of Ferozsons Laboratories Limited. the display returns to normal figure and the sterilizer begins adjusting to the defined set value. It cannot be published or divulged for whatever purpose to unauthorized persons. . QC – 047-01 Effective Date: Review Date: Page No: 6 of 12 Procedure For Operation & Cleaning of Universal Incubator UN-260 5. figure – 5a for bacterial incubation & figure – 5b for fungal incubation. Figure – 5a: Save the set value by pressing the knob Figure – 5b: Save the set value by pressing the knob Reviewed & Approved by: Sr.2. in any form without the prior written permission from DQO Ferozsons Laboratories Limited. or 3 rd parties.FEROZSONS LABORATORIES LIMITED Standard Operating Procedure SOP No. COPY NO.

2. in any form without the prior written permission from DQO Ferozsons Laboratories Limited. Figure – 6: Switch-off the Incubator 5.9 Open the door completely by pulling the door handle from right side to the left. see figure – 6.FEROZSONS LABORATORIES LIMITED Standard Operating Procedure SOP No.2. MQA Sign: Date All or part of the information contained in this document should be treated as the confidential property of Ferozsons Laboratories Limited. .8 Turn the power switch off by pressing the On/Off switch on the front of the incubator after setting the temperature parameters. QC – 047-01 Effective Date: Review Date: Page No: 7 of 12 Procedure For Operation & Cleaning of Universal Incubator UN-260 5. figure – 7. COPY NO. Figure – 7: Opening the door Reviewed & Approved by: Sr. or 3 rd parties. It cannot be published or divulged for whatever purpose to unauthorized persons.

2.FEROZSONS LABORATORIES LIMITED Standard Operating Procedure SOP No.2. COPY NO.10 After testing desired materials incubate agar plates and broth media bottles properly. It cannot be published or divulged for whatever purpose to unauthorized persons. . Note: Do not load such materials which produce poisonous explosive vapors or gases. so that the air circulates properly inside the working chamber. or 3 rd parties. figure – 8.11 Do not load the chamber too tightly. place them carefully inside the incubator at the temperature ranges 20 °C – 25 °C for fungal microorganisms & 30 °C – 35 °C for bacterial microorganisms respectively. QC – 047-01 Effective Date: Review Date: Page No: 8 of 12 Procedure For Operation & Cleaning of Universal Incubator UN-260 5. 5. in any form without the prior written permission from DQO Ferozsons Laboratories Limited. the incubator may overheat and pose a fire hazard. Reviewed & Approved by: Sr. Figure – 8: Correct Placement of Chamber Load Note: If the door is open while the sterilizer is in operation. MQA Sign: Date All or part of the information contained in this document should be treated as the confidential property of Ferozsons Laboratories Limited. if there is any doubt then they must not be loaded into to the incubator. also do not place any material on the chamber floor or touching the chamber side walls. Do not leave the door open during operation.

. QC – 047-01 Effective Date: Review Date: Page No: 9 of 12 Procedure For Operation & Cleaning of Universal Incubator UN-260 5. It cannot be published or divulged for whatever purpose to unauthorized persons. in any form without the prior written permission from DQO Ferozsons Laboratories Limited. MQA Sign: Date All or part of the information contained in this document should be treated as the confidential property of Ferozsons Laboratories Limited.2.12 After loading the desired materials into the incubator. figure – 9.FEROZSONS LABORATORIES LIMITED Standard Operating Procedure SOP No. or 3 rd parties. COPY NO. Figure – 9: Closing the door Reviewed & Approved by: Sr. push the door handle from left side to the right for closing the door.

3 MAINTENANCE & CLEANING PROCEDURE 5. It cannot be published or divulged for whatever purpose to unauthorized persons.6 Grease the moving parts of the door (hinges and lock) with thin silicone grease and check that the hinge screws are not loose. 5.3 Risk of drying material catching fire.2 Safety Precautions: 5. or 3 rd parties. 5.2. 5.4.3. MQA Sign: Date All or part of the information contained in this document should be treated as the confidential property of Ferozsons Laboratories Limited. the affected area must be immediately cleaned and polished.4.2 Risk of melting plastic objects causing fire. such as concentrated acid.4 Do not use benzene or thinner or any volatile substances to clean the body as these substances may deteriorate the body or strip the paint.4 Risk of electrical shock.4. if sensor comes loose from the fixing clip. be careful not to damage this sensor during cleaning the chamber. QC – 047-01 Effective Date: Review Date: Page No: 10 of 12 Procedure For Operation & Cleaning of Universal Incubator UN-260 5.1 Risk of burns from hot incubators.1.4. 5. 5. and wipe off the stains with it. 5. salts & alkalis. Reviewed & Approved by: Sr.1.1. 5.1 Hazards to Staff: 5.3. reattach it.4 ASSOCIATED RISKS & SAFETY PRECAUTIONS 5. in any form without the prior written permission from DQO Ferozsons Laboratories Limited. 5.1.3.5 If rust spots appear on the surface of the working chamber due to impurities. 5.4. and also wipe off any moisture with a dry cloth.3.3.4. 5.1 Be sure to start maintenance or service work after the incubator has been unplugged.FEROZSONS LABORATORIES LIMITED Standard Operating Procedure SOP No.2.4.2 A temperature sensor is installed near to ventilation flap at the top of the chamber.3. . once a year.4.2 Take care when operating chamber as electric shock may be hit when opened. wring a cloth moistened with neutral detergent diluted with water. COPY NO. 5. to remove stubborn stains.1 The incubator chamber is made of stainless steel never attempt to use liquids containing substances that corrosive to stainless steel.3 Gently wipe stains from the body with a soft cloth.

5 Heat Labile plastic objects do not be placed inside the incubator. FZ-203 (Temperature Monitoring Cycle = 20 – 25 °C) c) Form No. COPY NO. 5.4 Do not pile specimen on top of one another. TRAINING Training of the concerned SOP shall be given to the personnel who will be exercising the SOP.2. in any form without the prior written permission from DQO Ferozsons Laboratories Limited. MQA Sign: Date All or part of the information contained in this document should be treated as the confidential property of Ferozsons Laboratories Limited. – 1.5 DOCUMENTATION Sterilization cycles (time) & cleaning entries are written in the following LOGs: a) LOG Sheet No.3 Be careful not to pinch hands when closing the door. It cannot be published or divulged for whatever purpose to unauthorized persons.4. REFERENCE British Pharmacopoeia – 2017. FZ-203 (Temperature Monitoring Cycle = 20 – 25 °C) 3) Form No. 7. Memmert Manufacturer’s Manual United States Pharmacopoeia – USP 40. NF 35 – 2017. or 3 rd parties.2. and will be documented on the attached appendix-II. 6. District Laboratory Manual – Monica Cheesebrough – Vol. 5. FZ-202 (Temperature Monitoring Cycle = 30 – 35 °C) b) LOG Sheet No. Reviewed & Approved by: Sr. APPENDICES 1) LOG Sheet No. QC – 047-01 Effective Date: Review Date: Page No: 11 of 12 Procedure For Operation & Cleaning of Universal Incubator UN-260 5.FEROZSONS LABORATORIES LIMITED Standard Operating Procedure SOP No. 5. FZ-204 (Cleaning Record) Retention Period: The original reports will be retained in Microbiology Section for Three (3) years.4. sufficient air should be allowed to circulate round the material so that heat does not build-up and the materials combust. FZ-202 (Temperature Monitoring Cycle = 30 – 35 °C) 2) LOG Sheet No.2. . FZ-152 (Cleaning Record) 3) Training Record 8.4.

09 October. COPY NO. DISTRIBUTION AND RETRIEVAL Distribution Retrieval Title Signature Date Copy No.FEROZSONS LABORATORIES LIMITED Standard Operating Procedure SOP No. QC – 047-01 Effective Date: Review Date: Page No: 12 of 12 Procedure For Operation & Cleaning of Universal Incubator UN-260 9. HISTORY Date Reason of Change Change of Standard Operating Procedure format. 2017 Inclusion of Pictures & Glossary. 10. It cannot be published or divulged for whatever purpose to unauthorized persons. or 3 rd parties. in any form without the prior written permission from DQO Ferozsons Laboratories Limited. Signature Date Reviewed & Approved by: Sr. th Revision of Standard Operating Procedure and Reference. . MQA Sign: Date All or part of the information contained in this document should be treated as the confidential property of Ferozsons Laboratories Limited.

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