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Konelab
CHOLINESTERASE with Dibucaine/
981382 10 x 10 ml
H2O2 + + 4-AAP ---

in vitro
510 nm.

(EC 3.1.1.8, SChE) ,
, Konelab. 10 x 10 ml
10 x 10 ml
. :
(1, 2) 100 mmol/l
10 mmol/l
. 1 mmol/l
"" . 4- (4-AAP) 0.4 mmol/l
, , 1300 U/l
, 150 U/l
. , 0.35 mmol/l

. in vitro .
(SChE).
, , , .
.

, .
30 min .
. :
(),
. Konelab.
,

. 2...8 C
.
, Konelab

.
5
. 28 C.
SChE
.
u a f s
E1 , E1 , E1 E1 . 25 .
u u
E1 E1 . .
a
E1 .
. (2)
.
f
E1
s
. E1
.
()
. (5)
, 1
SChE 2025 C 48 C 12 -20 C.
SChE.

,
:

> 70%: Konelab.
SChE.
Thermo Electron Oy,
40-70%:
SChE .
.
< 30%:
.
.
(2, 3, 4)
.
"" .
"" , Cholinesterase test kit ( ) (:
. 981370)
.

(Template: D01162_2.dot)

+ H2O--- + (dA/min)
.
+ O2 --- + 2 H2O2 eCal (981830)

eCal (981830).

2003 Thermo Electron Corporation

All rights reserved.
Trademarks are the property of
Thermo Electron Corporation and
its subsidiaries.

D02404_B_B_insert_Cholinesterase with
Dibucaine_EL.doc
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340 U/l. 938 U/l.
SD CV% SD CV%
.
14.5 5.1 13.5 1.4
:
Nortrol, : 981043 4.1 1.4 26.8 2.8
Abtrol, : 981044 17.4 6.1 31.0 3.3

. NCCLS EP5-A Konelab 30
( ) 10 , n = 40.

.

79 88
Konelab : 71 75
82 97
U/l = x (dA/min)

TV x 1000 (n = 30)
= - , .
6.89 x SV x P
.
: TV= ml
SV= ml
6.89= 1. Burtis, CA and Ashwood, E R (ed.), Tietz Fundamentals of Clinical
510 nm Chemistry, 5th edition, W B Saunders Company, Philadelphia, 2001,
P= = 1 pp. 385-387.
2. Thomas, L. (ed.), Clinical Laboratory Diagnostics; Use and
st
kat/l = (U/l)/60 Assessment of Clinical Laboratory Results, 1 edition, TH-Books
Verlagsgesellschaft mbH, Frankfurt/Main, Germany, 1998, pp. 65-71.
3. Okabe, H., Sagesaka, K., Nakajima, N. and Noma, A., New

Enzymatic Assay of Cholinesterase Activity. Clinica Chimica Acta, 80
: (1977), 87-94.
4. Panteghini, M. and Bonora, R. Evaluation of a New Continuous
. = 100 - x 100 Colorimetric Method for determination of Serum
Pseudocholinesterase Catalytic Activity and its Application to a
Centrifugal Fast Analyser. J. Clin. Chem. Clin. Biochem. Vol. 22,
1984, pp. 671-676.
5. Guder WG, Narayanan S, Wisser H, Zawta B. List of Analytes;
. Preanalytical variables. Brochure in: Samples: From Patient to the
. Laboratory. GIT Verlag GmbH, Darmstadt, 1996.
6. Young, D.S., Effects of Drugs on Clinical Laboratory Tests, Fifth
Edition, AACC Press, Washington, D.C., 3-4 3-5, 2000.
: 10% .
: 800 Thermo Electron Oy
mol/l (47 mg/dl ).
Clinical Chemistry and Automation Systems
: 10 g/l . Ratastie 2, P.O. Box 100, FI-01621 Vantaa, Finland
: 11.3 mmol/l
Tel. +358 9 329 100, Fax +358 9 3291 0300
(1000 mg/dl) . www.thermo.com/konelab
.
, (--)
6. 2004-06-30


/ ,
51

, :



79
71
C
83
Sd 2.1

(2).

.

.

75 - 3500 U/l (1.3 58 kat/l)
: 75 -
10500 U/l (1.3 -175 kat/l)


37 U/l (0.6 kat/l)

/
.
+ 3 SD ( , n=24).

D02404_B_B_insert_Cholinesterase with
Dibucaine_EL.doc