RESULT

Table 1: Glucose Standard Curve

Concentration (g/L) Absorbance (/) Amylase Activity (1U)

0 0 0
0.2 0.1 6.667x109
0.4 0.255 1.333x108
0.6 0.391 2x108
0.8 0.539 2.667x108
1 0.627 3.333x108

Y = ABSORBANCE

X = CONCENTRATION

Concentration (g/L) VS Absorbance (/)

0.7
y = 0.6554x - 0.009
0.6
Y = 0.180
0.5
Absorbance (/)

0.4
X = 0.2884
0.3

0.2

0.1
Concentration (g/L)
0
0 0.2 0.4 0.6 0.8 1 1.2
-0.1

Figure 1: Standard Curve Calibration

 Table 2: Effect of Substrate on Enzyme Activity

CONCENTRATION OF ABSORBANCE (g/L) Amylase Activity (1U)

STARCH SOLUTION (%)
0.5 0.063 1.667x108
1 0.064 3.333x108
1.5 0.135 5x108
2 0186 6.667x108
3 0.246 1.0x107

Concentration Of Starch Solution (%) VS Absorbance (/)

0.3
Y = 0.180

0.25 y = 0.0804x + 0.0101

X = 2.1132
0.2
Absorbance (/)

0.15

0.1 Concentration (% )

0.05

0
0 0.5 1 1.5 2 2.5 3 3.5

Figure 2: Absorbance VS Substrate Concentration

 Table 3 : Effect of pH on Enzyme Activity

pH Absorbance (/) Amylase Activity (1U)

5 0.099 3.3103
7 0.093 3.1103
8 0.054 1.8103
9 0.013 4.33104
11 0.019 6.33104

pH (/) VS Absorbance (/)

0.12

0.1 Y = 0.180

0.08
Absorbance (/)

X = 0.225

0.06

0.04

0.02

y = -0.016x + 0.1836
pH (/)
0
0 2 4 6 8 10 12

Figure 3 : pH Versus Absorbance