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Activated precursors (ATP, GTP, UTP, CTP) c. Divalent metal ions (Mn2+ , Mg2+ ) d. Primer 2. Which one is the segment of a eukaryotic gene that is not represented in the mature m-RNA ? a. Intron b. Exon c. Plasmid d. TATA box 3. The similarity between vit C and vit K is: a. Both are involved in coagulation cascade b. Both help in post-translational modification c. Both help in conversion of praline to hydroxyproline d. Both have anti infective activity. 4. _______element is known to influence the body’s ability to handle oxidative stress. a. Calcium b. Iron c. Potassium d. Selenium 5. Which is the enzyme that makes a double stranded DNA copy from a single stranded RNA template molecule? a. DNA Polymerase b. RNA Polymerase c. Reverse Transcriptase d. Phosphokinase 6. Which separation technique depends on the molecular size of the protein? a. Chromatography on a carboxymethyl cellulose column b. Iso-electric focusing c. Gel filtration chromatography d. Chromatography on a diethylaminoethyl cellulose column 7. Which one of the following is product of β-oxidation of odd-chain fatty acids? a. Succinyl CoA b. Propionyl CoA c. Acetyl CoA d. Malonyl CoA
Which one of the following is true for Nitric Oxide Synthase? a. FAD. Phenylalanine is not the precursor of : a. Which one of the following is best parameter to monitor Thiamine level? a. It is inhibited by Ca++ b. c. Reticulocytosis d. G6PD activity . Ligase chain reaction c. All are actions of insulin EXCEPTa. lipogenesis b. glycolysis c. FMN and Heme iron. It requires NADPH. Lysine c. Tyrosine Kinase b. Tyrosine d. Thyroxine 14. gluconeogenesis 10. Histidine b. ATP synthase d. ______ is a membrane bound enzyme that catalyzes the formation of cyclic AMP from ATP. 11. It accepts electrons from NADH. glycogenesis d. Thiamine level in blood c. Melatonin c. Keratin 12. Epinephrine b. Polymerase c. DNA Sequencing 13. Adenylate cyclase 9. Polymerase chain reaction d. Single strand conformational polymorphism b. Which one of the following cannot be used to detect mutation? a. It catalyzes a dioxygenase reaction. a. Transketolase level in blood b.8. Cysteine d. Which one of the following amino acid is responsible for elasticity of the corneal layer of skin? a. d.
In competitive inhibition the relation between Km and Vmax is one the following: a. Cleavage of peptide bonds b. Ceruloplasmin b. Lysol hydroxylase b. Km decreases and Vmax increases. Fatty acids b. Km and Vmax decreases c. Which one of the following is non vitamin co enzyme? a. Km and Vmax are the same b. Copper is required in collagen synthesis for: a. Xanthine oxidase b. Amino acids d. Glucose c. Creatine kinase 20. Cytochrome oxidase c. 21. S-adenyosyl methionine b. ______ is an example of antioxidant endogenous enzyme. Albumin c. Casein d. All 16. Niacin c. Histone 18. CoA d. Prolyl oxidase d. a. Fluorescamine is used to detect: a. Loss of tertiary structure c. Liberation of amino acids 19.15. Loss of sulfhydryl groups d. Which one of the following is an example of phosphoprotein? a. Km increases and Vmax remains the same d. Superoxide dismutase d. why most of the mammalian enzymes become inactive? a. At higher temperature. Prolyl hydroxylase 17. Lipoic acid . Lysol oxidase c.
UTP. RNA polymerase holoenzyme requires the following: Template of double stranded DNA Four ribonucleotide triphosphates GTP. Lactate. a. Kidney damage 25. This primary transcript undergoes extensive processing which involves cleaving out of the intervening introns (non coding sequence) and splicing (ligation) together of the exons (coding sequences). ans is A ( Introns ) The primary RNA transcripts contain two types of sequences: Introns (intervening sequences): sequences that do not code/ contribute to the genetic information ultimately translated into the amino acid sequences of protein molecule Exons (expressed sequences): sequences that code for various amino acids. Electrical cardioversion d. Glyceraldehyde-3-phosphate Dehydrogenase d. .22. Supplementation of Vitamin B12 and folic acid in megaloblastic anemia leads to the improvement of anemia due to: a. Erythroid hyperplasia 24. Oxidases b. Increased iron absorption d. Increased DNA synthesis in bone marrow c. _______ is an enzyme involved in the catabolism of fructose to pyruvate in the liver. CTP Mn2+ or Mg2+ 2. ATP. a. Increased Haemoglobin production b. Lysases 23. Glucokinase c. Peptidases d. ans is D ( Primer) RNA polymerase does not require a primer to initiate transcription while DNA polymerase requires it. _______ are the enzymes playing role in transfer of one molecule to another. Phosphoglucomutase Answers with explanations 1. Brain Ischemia c. Level of creatinine kinase – I is found increased in : a. Transferases c. Myocardial Ischemia b.dehydrogenase b.
. ans is B (Post-translational modification) Vit C is required for post-translational modification of praline and lysine in collagen synthesis. Cyclic AMP acts by activating a cyclic nucleotide-dependent protein kinase (protein kinase A) which catalyzes the phosphorylation. Iso-electric focusing – separates proteins based on the iso-electric pH. 7. None of these has antiinfective properties. End product of β-oxidation of odd-chain fatty acid is Propionyl CoA. ans is B ( propionyl CoA) End product of β-oxidation of even-chain fatty acid is Acetyl CoA. ans is D (Adenyl Cyclase) Adenyl cyclase is an enzyme that occurs on the surface of cell membranes. 3. ans is C (Reverse Transcriptase) Reverse transcriptase is an RNA dependent DNA polymerase that produces double stranded DNA copies of RNA templates.Thus introns are removed from the primary transcript and exons are ligated together to form mRNA molecule. cell membranes. Vit K is required for post-translational modification of glutamate residues for generation of clotting factors. Only vit C helps in conversion of praline to hydroxyproline. 5. Cyclic AMP is an important second messenger that is formed from ATP by the action of enzyme Adenyl cyclase. lipid and nucleic acids from oxidant molecules. Glutathione peroxidase helps to protect proteins. ans is C (Gel filtration chromatography) Gel filtration chromatography – separates protein molecules based on their size (molecular weight). DEAE & CM). in which proteins are separated based on electrostatic attraction between charged molecules and oppositely charged groups on ion exchange resins (i.e. which is a feature of vit A mainly. ans is D (Selenium) Selenium in the form of selenocysteine is a component of enzyme glutathione peroxidase. 8. Ion-exchange chromatography – on carboxymethyl (CM) cellulose medium or diethylaminoethyl (DEAE) cellulose medium. 4. DNA polymerase – synthesizes DNA from DNA RNA polymerase – synthesizes RNA from DNA Phosphokinase – adds a phosphate moiety 6. Only vit K is involved in coagulation cascade(generates biologically active clotting factors).
Tyrosine is formed from phenylalanine. 10. Substrates: arginine. . NO synthase is very complex enzyme employing five redox cofactors NADPH. strong. 12. thyroxine and triiodo-thyronine. ans is B (melatonin) Melatonin is formed from tryptophan/serotonin and not from tyrosine/phenylalanine. 14. ans is A (transketolase level in blood) thiamine deficiency is diagnosed by an increase in erythrocyte transketolase activity observed upon addition of thiamine pyrophosphate. O2 Co-enzymes: FAD. norepinephrine. ans is B (ligase chain reaction) mutation screening techniques: cytogenic analysis fluorescent in situ hybridizatioin (FISH) southern blot polymerase chain reaction (PCR) reverse transcriptase PCR DNA sequencing Single-strand conformational polymorphism Denaturing gradient gel electrophoresis (DGGE) RNAse cleavage Restriction fragment length polymorphism (RFLP) 13.tetrahydrobiopterin. ans is D ( keratin) The keratinized stratum corneum is tough. flexible and elastic. Heme iron. FAD. ans is C (requires NADPH. Ca++ -independent enzyme 11. FMN.9. FMN. FMN. Its stability and integrity are due to cross linkages between the keratin molecules. Tyrosine is also a precursor for epinephrine.tetrahydrobiopterin Products: NO and citrulline Enzymes: Ca++ -calmodulin-dependent enzyme. ans is D (Gluconeogenesis) Insulin inhibits gluconeogenesis. (Insulin would not support anything that would tend to increase blood sugar levels) Insulin promotes uptake of glucose from the blood and drives it inside the cells for: Utilization for energy production – glycolysis Storage in the form of glycogen – glycogenesis Storage as lipids – lipogenesis. Heme iron) NO is formed by the action of the cytosolic enzyme NO synthase. NADPH. Heme iron. FAD.
These cross links are formed through action of Lysol oxidase. Tissues are protected from superoxide by superoxide dismutase. 17. which is a copper containing protein that converts E amino group to aldehydes. ans is D (lipoic acid) classification of coenzymes: for transfer of groups other than hydrogen sugar phosphates CoAsH Thiamine pyrophosphate Pyridoxial phosphate Folate coenzymes Biotin Lipoic acid For transfer of hydrogen NAD+. ans is C (superoxide dismutase) oxygen toxicity is caused by superoxide radicals. Km is unaltered. 18. ans is C (Casein) Casein and Ovovittelin are the two important group of phosphoproteins found in milk and egg-yolk respectively. ans is C (amino acids) Fluorescmine is a fluorescent dye so it is used to detect aromatic amino acid. NADP+ FMN.15. FAD Lipoic acid Coenzyme Q . both within and between triple helical units. ans is A (lysol oxidase) Collagen fibres are stabilized by formation of covalent cross link. 19. Cytochromes – hemoproteins acting as electron carrier Xanthine oxidase – used in purine and pyrimidine synthesis Creatine kinase – catalyze the formation of creatine phosphate. ans is C (Km increases and Vmax is the same) competitive inhibition – Km is high. ans is B (loss of tertiary structure) Increase in temperature disrupts the noncovalent interactions that maintains the enzyme three dimensional (tertiary) structure. 21. Vmax is same non competitive inhibition – Vmax lowered. 16. 20.
This prevents cell division and formation of nucleus of new RBCs leading to megaloblastic anemia. 23.22. 24. ans is A (increased DNA synthesis in bone marrow) Deficiency of Vit B12 leads to impaired purine and pyrimidine synthesis which leads to impaired DNA synthesis. ans is (Glyceraldehyde-3-phosphate dehydrogenase) Glyceraldehyde-3-phosphate dehydrogenase is an enzyme in the pathway for conversion of fructose to pyruvate. ans is B (transferases) Transferases are enzymes that catalyze the transfer of functional groups from one molecule to another. ans is B (brain ischemia) creatinine phosphokinase exists as three isoenzymes in human tissues: CPK 1 or CPK-BB: found in brain CPK 2 or CPK-MB: found in myocardium CPK 3 or CPK-MM: found in skeletal muscle 25. ==============X============== .
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