164 Incubation with anti-phosphotyrosine (Clone 4G10, Merck) or anti-tubulin

antibody (1:5000, Sigma, T6094) was performed over the same membranes
(stripped previously) and developed with anti-mouse conjugated to horseradish
peroxidase (HRP, 1:10000, BioRad 172-1011).

Qué sujeto tiene “developed”? A quién se refiere?

201 This time, it was enough to prove SPINK3 calcium inhibitory effect
according to time lapse imaging experiment design in single-cells

Este tiempo fue suficiente o esta vez fue suficiente?

219 Membranes developed???

246 and in fact, it effectively regulates both processes; or instead, this effect is
a consequence

Parecería que hay una contración con “in fact” y “instead”….

262 Upon BSA addition, we detected an increase in the calcium signal of living
cells (evidenced by beating flagella) after 20 seconds in cells and the highest
signal intensities were reached after 60 seconds post-stimulus

In cells está bien?

295 still BSA-responding cells showed reduced intracellular Ca2+ levels.
Still para decir Aún?

379 Besides that intracellular calcium mobilization is required in both

capacitation initiation and AR,

Está un poco confusa esta oración.

453 Likewise, the fact that SPINK3 binds both the head sperm and midpiece to
unknown ligand, would suppose that ???? (sujeto???) could be regulating
some calcium receptor present in these regions

477 head sperm region during capacitation no es sperm head region???

493 reaches in the ampulla ???

389 Data was expressed as percentage of cells reacted (o cells´reaction) with

respect to total + standard error (n=200 per replicate, 3 independent replicates).
796 Acrosome loss was quantified under microscope …..quantified or
measured ???