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Methods Washing membranes with water 4. Easy to navigate image of the sampled microenvironment. Magnus Wetterhall 1. Focused on one message 2. 61 In this study. Captures attention 2. Klas Hjort. resulting in Typography >3 a non representative protein 200µL No Navigation NH4HCO3 5. Marcus O. The dynamic and complex protein adsorption5 affects the composition of the collected dialysate. protein adsorption onto the membrane is a drawback when proteins are sampled in vivo3. protein Colored badly 4. Sjödin. Not too many colors 10µL DTT 15 min 50 OC Lack of structure Text size small Too much text Also called template poster Unreadable Blurry images No consistence A good and beautiful poster also 1. Jonas Bergquist.. Not too much text (300-800 words) Typography adsorption onto the membrane is a drawback Bad Dry under vacuum when proteins are sampled in vivo3. Science Communication main communication channels Level of details main communication aids Abstract main communication visuals Introduction Oral Method Article Results Poster Discussion Aid Text Visuals Talk Conclusions References Article Oral Poster Schematic Photo Charts Protein Visuals Membrane Levels of poster design IMPACT No design Template poster Communicative poster Fast to make Does not scare away and Draws attention and holds would likely be read if the audience interest long How visuals are used in scientific presentations someone finds it enough to convey the information Nobody will read it Nobody will notice it Takes some time to do LEVEL OF DESIGN Journal articles Oral presentations Poster Good and beautiful poster “A poster must do two things well: to be noticed and to hold your attention long enough to get the message across. Protein adsorption on surface 55 44 12 Many focal points Space Balanced modified MD catheters is compared with routine microdialysis membranes using an in vitro sampling. Conclusions Microdialysis is one of the few real time sample Unbalanced Unified Colored collection methods routinely used in clinical neuro-surgical research1. However. The dynamic and complex protein 26 Ugly Beautiful adsorption5 affects the composition of the collected dialysate.4. Introduction 3. However. Conveys information 4.2. Results 1. Lars Hillered.D.. Controls eye movement 3. Organized Microdialysis is one of the few real time sample collection methods routinely used in clinical neuro- surgical research1. resulting in a non representative protein 51 7 No focal point Focal point 2. Evokes emotion Also called communicating poster . we present a method to analyze protein adsorption from complex samples on small and delicate surfaces. and A poster should be seen as in that order” Emil Weiss (1896-1965) • Source of information Navigation • Conversation starter Structure Text size • Advertisement of your work Consistence Good • Summary of your work Communicative Multiplexed Quantification of Proteins Adsorbed to Surface Modified and Non Modified A good poster is Microdialysis Membranes Andreas P Dahlin.4.2. No space 3.

Introduction 2.D.7 Counts 2 3.D. Marcus O. Klas Hjort.8 2.1 81. Methods Microdialysis is one of the few real time sample collection methods routinely used in clinical neuro- Microdialysis is one of the few real time sample Sequential Organic Miniprojects surgical research1.4.D. Helvetica) are best for headlines • Serif fonts (like Times. Results Natural reading order Portrait Landscape Multiplexed Quantification of Proteins Adsorbed to Surface Modified and Non Modified Multiplexed Quantification of Proteins Adsorbed to Surface Microdialysis Membranes Modified and Non Modified Microdialysis Membranes Andreas P Dahlin.2 30 . Methods Introduction Methods 1 Introduction 2 Methods Introduction Methods Introduction Methods Introduction Methods . • Edit text • Main headline should be larger than 90pt • Remove redundant images • Sub-headings should be at least 36pt • Text should be at least 24pt 3 weeks • Keep text elements to 50 words or fewer • Use phrases rather than full sentences • Send out the draft to your peers and ask • Keep the length of the line to approx. Sjödin.65 modified MD catheters is compared with routine 0 0 -1.7 6. protein adsorption onto 100 120 *** ** 0.D. Klas Hjort. Jonas Andreas P Dahlin. Garamond) are good for block text 2 weeks Graphics • Edit information. Protein adsorption on surface Membrane Dialysate adsorption from complex samples on small and Min: -1. Jonas Andreas P Dahlin.5 7. Magnus Wetterhall 2.D.2.4 12 11% dialysate.4. Klas Hjort.41 -0. Methods 2. Sjödin. Methods 20 -1. Results 3. Sjödin.8 adsorption from complex samples on small and In this study. Jonas Andreas P Dahlin.0 29 20 . Lars Hillered.3 Counts membranes with water 2 Microdialysis is one of the few real time sample collection methods routinely used in clinical Membrane neuro-surgical research1. protein Multiplexed Quantification of Proteins Adsorbed to Surface Multiplexed Quantification of Proteins Adsorbed to Surface Multiplexed Quantification of Proteins Adsorbed to Surface A) B) C) CSF 0-9% 10 44. Methods 2.2 Counts * * 10 . Lars Hillered. Sjödin. Methods Introduction Methods 1.4 3% 12 11% 10 47.9 73.6 67.454 49. Magnus Wetterhall Bergquist.2 the membrane is a drawback when proteins are sampled in vivo3. Results 3.0 20 . Klas Hjort. E-mail. Magnus Wetterhall Andreas P Dahlin.5 0.6 40 40 31% 4% 21% NH4HCO3 2 20 -0. Results 1. 55 characters (10-15 words) for feedback on the information only for fast reading • Refine images: Unify them with the poster • Left justify the text • Add color scheme • Choose typography • Do not use more than 3 typefaces • Sans serif fonts (like Arial.4 5. Marcus O.61 -0. Introduction Development of 2.0 delicate surfaces.29 % 2 Andreas P Dahlin. Marcus O.7 Counts start start 2 10 50 110 4.5 7.0 20 40 75 50 75 100 Min: -1.503 44.72 -0.5 0. resulting in a non representative protein Dry under DTT 60 9% membranes Movement S-curve Semi-circle One idea . Lars Hillered. Magnus Wetterhall 4 14 Instability index composition of the collected dialysate. Results 3. Jonas 21% and complex protein adsorption5 affects the 80 -0.19 % 0. Methods 2.2 dialysate.0 20 40 75 50 75 100 Molecular weight (kDa) Isoelectric point (pI) GRAVY score Instability index Aliphatic index end Navigation on a poster is crucial to make it easier for the audience to read the poster in correct order end Radial Importance Clock Conclusion-driven How to show reading order Strong focus Strong focus Start close to end in space Numbers Most obvious navigation aid Arrows Also great navigation tool Numbers and arrows Best solution 1.454 49.D. The dynamic 80 100 0.7 6. modified MD catheters is compared with routine microdialysis membranes using an in vitro sampling. make it more effective • Good graphs communicate relationships quickly • Decide poster focal point • Graphs should be simple and clean  • Decide reading order • Write explanations directly on figures. Lars Hillered. resulting in Aliphatic index GRAVY score 60 40 .3 4.0 9. Lars Hillered.326 39. Magnus Wetterhall Bergquist. and own the venue Multiplexed Quantification of Proteins Adsorbed to Surface Multiplexed Quantification of Proteins Adsorbed to Surface Multiplexed Quantification of Proteins Adsorbed to Surface More creative reading order Modified and Non Modified Microdialysis Membranes Modified and Non Modified Microdialysis Membranes Modified and Non Modified Microdialysis Membranes Andreas P Dahlin.0 -0. Protein adsorption on surface CSF Membrane Dialysate CSF Membrane Dialysate CSF Membrane Dialysate projects microdialysis membranes using an in vitro sampling. Jonas Andreas P Dahlin. However.39 % Dialysate Bergquist. Magnus Wetterhall Bergquist. Klas Hjort. Sjödin. Marcus O. Magnus Wetterhall Bergquist. images etc. Marcus O. instead of referencing from • Add navigation cues elsewhere • Rearrange objects • Test the poster on your peers and on Layout someone not in science • Use visual grammar to guide readers to the important parts of 1 week your poster • Make final adjustments • Balance the placement of text and graphics to create visual • Prepare for print appeal • Print large poster and small handouts • Use white space creatively to help define the flow of information Will the reader be able to contact you? • Add Names. Outshine your neighboring posters. Marcus O.8 5.0 -0. -0. Jonas Organize Bergquist. Jonas end end end Bergquist. Results Washing 10 37. Methods 2. Methods 11. Marcus O.100 % -0. Klas Hjort. Results 3.2. The dynamic and complex protein 21% 30 . webpage….326 39. typography etc • Want to collaborate? • Something else? Organize 4 weeks • How should the reading order be? • Start to draw in your software of choice • How could I help the audience to navigate the poster? and create a first draft • Add guiding grids Text • Add headings.7 adsorption onto the membrane is a drawback Modified and Non Modified Microdialysis Membranes Modified and Non Modified Microdialysis Membranes Modified and Non Modified Microdialysis Membranes *** * * 100 120 Dry under *** ** 0.61 -0. Klas Hjort.5 -1. addresses. Lars Hillered.503 44.41 -0.9 73. we present a method to analyze protein 20 20 -1. we present a method to analyze protein 40 image of the sampled microenvironment. text.many sub- vacuum NH4HCO3 15 min -0.0 Membrane Dialysate 10 50 110 4.72 -0. Conclusions 10 Counts 2 Membrane 10 44.100 % 10µL 60 3% Washing 200µL image of the sampled microenvironment. Sjödin. Magnus Wetterhall 1.D.65 0 0 -1. However. Sjödin. Lars Hillered.6 67.1 81.6 42 5 28 with water In this study. 50 OC 40 31% 4% 21% -0.19 % vacuum when proteins are sampled in vivo3.D.90 Min: -1.2.8 5. resulting in a non representative protein 80 4 14 Instability index Aliphatic index GRAVY score adsorption5 affects the composition of the collected 40 . Conclusions CSF 3. Methods start start start 3.2 delicate surfaces. Introduction 3. protein adsorption onto collection methods routinely used in clinical neuro- the membrane is a drawback when proteins are A) B) C) CSF 0-9% *** * * surgical research1.4.4 5. The dynamic and complex protein * * 10 . Sjödin.29 % 100 adsorption5 affects the composition of the collected 80 29 sampled in vivo3. Marcus O. Results Dialysate 10 47.2 Molecular weight (kDa) Isoelectric point (pI) GRAVY score Instability index Aliphatic index CSF Membrane Dialysate CSF Membrane Dialysate CSF Membrane Dialysate 10µL DTT 15 min 50 OC A) MW B) pI C) GRAVY D) Instability E) Aliphatic CSF 37.5 -1. Poster time table Good Poster 5 weeks • Define the message you want to deliver Define the purpose and prepare • Check poster size and recommendations What do you want the person passing by your poster to do? • Draw the layout on a piece of paper • Engage in a discussion about the content? • Search for inspiration and ideas for • Learn enough to go off and try for themselves? color schemes. Lars Hillered.39 % -0. Methods A) MW B) pI C) GRAVY D) Instability E) Aliphatic 4. Social media.7 60 9% a non representative protein Counts 42 5 28 200µL start -0.0 9. Jonas Bergquist. However.90 Min: -1. Klas Hjort. Introduction 2. Introduction 22. 2.

Phasellus accumsan. al 2012]. Process image of the text Washing Simple flow diagram.adobe. consectetur adipiscing elit. not in Visuals Membrane µL 0. the membranes were excavated from the catheters.Y.com • 8 % of all men Test your images at: Basic color wheel • 0.32 and 1. Helvetica) are best for headlines • Serif fonts (like Times.” “Lorem Ipsum comes from sections 1. faster read vacuum NH4HCO3 15 min 50 OC with water Good in posters but quite boring Faster communication with image and text combined Creativity Detailed flow diagram with added 3D-visuals.1M NH4HCO3. N. Lorem Ipsum has been the 86 words 86 characters/line industry's standard dummy text ever since the 1500s. remaining essentially unchanged. in euismod mauris maximus vel. when an unknown printer took a galley of type and scrambled it to make a type specimen book.com Sans serif font • Use images with resolution > 300 dpi Graphics Types of graphics in science • • Use headlines above figures instead of figure captions  Write explanations directly on figures. Size Text Choose Typeface Type affects the perception of credibility Choose it wisely Main headline should be larger than 90pt • Do not use more than 3 typefaces Title • Sans serif fonts (like Arial. It has survived not only five centuries.. Thereafter.2 line space Lorem ipsum dolor sit amet. Georgia DIN Clarendon Hipster Trend Best for: Block text Head lines Head lines Drop caps Ornaments About Lorum Ipsum “Lorem Ipsum is simply dummy text of the printing and typesetting industry. written in 45 BC” Left and right justified Source: Lipsum.10. Donec in nulla quis nisl accumsan cursus. Hamburg. Communicate fast due to object recognization. ipsum dui Family A A A A A Serif Serif Sans serif No Serif Slab serif Fat Serif Script Decorative faucibus ipsum. Vivamus Examples Times Arial Rockwell Allura Broadway dictum efficitur ante.com Extended color wheel 99% have red-green color deficiency . USA).5 µL/min Schematic Photo Charts during 12 h.5 mL protein low binding vials (Eppendorf.33 of "de Finibus Bonorum et Malorum" (The Extremes of Good and Evil) by Cicero. Garamond) are good for block text 90pt Pairing fonts Headline: Serif (Baskerville) Headline: Slab serif (Rockwell) Headline: Sans Serif (DIN) Sub head: Sans serif (DIN) Sub head: Sans serif (DIN) Sub head: Serif (Baskerville) Sub-headings should be at least 36pt Figure: Sans serif (DIN) Figure: Sans serif (DIN) Figure: Serif (Baskerville) Subtitle Text should be at least 24pt 36pt Text 24pt Block text Typeface differences < 50 words/block 55 characters/line 1. transferred to 0. Left justified Praesent in nunc at augue dignissim mollis.10. The disulfide bridges in the proteins were reduced by adding 10 μl of posters 45 mM DTT and incubating for 15 min at 50°C [Dahlin et. Photo Best for poster Schematic Charts Clear and crisp High data-to-ink ratio Washing Dry under 200µL 10µL DTT Effective No shadows membranes vacuum NH4HCO3 15 min HIGH with water 50 OC Label items No 3D effects Memorable No textures Color harmonies Color coding No gradients RGB =Red Greed Blue Colors Screen color system CMYK = Cyan Magenta Yellow Black (Kelvin) Printer color system HSV = Hue Saturation Value Color Lightness Darkness HEX = Hexadecimal 6-digit web code for color #XXXXXX Monochromatic Complementary Triadic Tetriadic Analogous Color deficiencies FInd color palettes at: • 8 kinds of color deficiencies color.0 line space Avoid Comic Sans electronic typesetting. the microdialysis catheters were washed externally and and attractiveness internally by placing them in water and rinsing with water at a flow rate of 0. but also the leap into 1. justo vel ullamcorper pretium. Germany) and dried using a Text with details (93 words) LOW Protein Speedvac system ISS110 (Savant Holbrook. instead of referencing from elsewhere Graphics for fast communication Text in ‘method section’ of article Communication speed After the sample collection. Graphical design creativity membranes Dry under 200µL 10µL DTT Few details. All samples were redissolved in 200 Perfect in articles.5% of all women color-blindness. Baskerville Futura Glypha Tangerine Tarzan Serif font Praesent eu vestibulum. id pharetra neque nunc nec sem.

Balance title section with a bottom section No space = Light Low = Light Low = Light Straight = Light Simple = Light . Simple poster balancing At edge = Heavy Large = Heavy Complex = Heavy Many = Heavy Centre = Light Small = Light None = Light Few = Light Isolation Value Contrast Orientation Shape 1. Layout Use visual grammar to guide readers to the important parts of your poster Start by utilizing CRAP method C = Contrast Very disturbing R = Repetition A = Alignment P = Proximity Poor contrast = poor readability Very disturbing Repeat visuals to create a sense of Align elements properly. line thicknesses.com) The more an element attracts the eye. This helps develop the makes the poster more difficult to • Light text on light background organization and strengthens the understand • Too similar fonts unity Composition funnel Emphasis Entry point to poster Structure Negative space = Areas without information Where to place visuals Positive space = Areas with information Equally important Balance Harmony of poster Natural emphasis elements High contrast Saturation Negative space (empty space) Emphasis Camera focus Motion • Lungs of good design • Not an indication of insufficient information Faces or figures • As important as areas with information • The first thing you see on the poster Influencers • Strive for large areas of empty space • • The graphical element that draws the attention Guiding lines Framing Better with smaller text size and more space around than the opposite Geometry How to create effective visual emphasis 1 2 3 4 Structure Examples of poster structures Symmetric Radial symmetric Asymmetric Rule of third Boxed shape Boxed shape Organic shape Organic shape Space around Space around Space around Differentiating color 5 6 7 8 Organic shape Organic shape Organic shape Organic shape Space around Space around Space around Space around Easy to do Strong focus Easy to do Interesting Differentiating color Differentiating color Differentiating color Differentiating color Harmonious Harmonious Easy to fit info Strong focus Between text boxes Between text boxes Between text boxes Between text boxes Adjusted position Adjusted position Adjusted position Significantly larger Significantly larger Uninteresting Reading order? Uninteresting Difficult to fit info 3D perspective Difficult to fit info Difficult to fit info Unbalanced Non-template structure Balance “Balancing a composition involves More advanced poster balancing 1. Position Size Texture Quantity areas. Check that you have equal amount of images and text in each section Isolated = Heavy High = Heavy High = Heavy Tilted= Heavy Complex= Heavy 3. Poor alignment disturbs harmony and • Dark text on dark background sizes. etc. This is Grouping = Place elements that = annoying = unprofessional togetherness especially important for text boxes belong to each other close Avoid Very disturbing vertically and horizontically Create more negative space = • Background picture You can repeat color. texture. shape. Begin by balancing your emphasis point Defining visual weight negative space in such a way that no one area of the design overpowers other Visual weight chart Visual weight denotes the impact of a graphical element.” Steven Bradely. Determine the visual weight of your visual elements arranging both positive elements and 2. (smashingmagazine. the heavier its visual weight. Divide your poster in 4 sections 2. breathing room • Background gradient spatial relationships.