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Disusun Oleh:
Kelompok 7 Tingkat 1 B
1. Dinda Rizqa Rachma A.
2. Hermanita Apriyanti
3. Mutia Shella Sevita
4. Rizka Mustika Rani


The Waterbath is an instrument used in the laboratory for carrying out serological,
agglutination, inactivation, biomedical, and pharmaceutical tests and even for industrial
incubation procedures. In general they use water, but some baths use oil. The temperature
range at which waterbaths are normally used range between room temperature and 60 °C.
Temperatures of 100 °C can be selected, using a cover with special characteristics. Water
baths are manufactured with chambers of a capacity ranging from 2 to 30 litres.
A waterbath is laboratory equipment made from a container filled with heated water.
It is used to incubate samples in water at a constant temperature over a long period of time.
All water baths have a digital or an analogue interface to allow users to set a desired
temperature. Utilisations include warming of reagents, melting of substrates or incubation of
cell cultures. It is also used to enable certain chemical reactions to occur at high temperature.
Waterbath is a preferred heat source for heating flammable chemicals instead of an open
flame to prevent ignition. Different types of water baths are used depending on application.
For all water baths, it can be used up to 99.9 °C. When temperature is above 100 °C,
alternative methods such as oil bath, silicone bath or sand bath may be used.
Similar to Heating Blocks, Waterbaths are also used for incubation purposes and
others, or can even replace heating blocks. The only difference is that there is a medium for
heating. A tool that serves to heat the air which is useful to keep the temperature stability of
the liquid in the test tube This tool is not equipped with Fuse, so it is less secure. The
principle of waterbath is When the switch is shifted in position on, then the electric current
from the source will provide suplly electricity on the heater. Heaters that are given an electric
current will provide heat to the appliance, the higher the temperature. Thermostat sensors
placed in the warming areas of the waterbath will also heat up and expand the liquid in the
sensor. At a certain temperature, where the expansion of the sensor is high enough, the
increased volume of this sensor fluid will put pressure on the thermostat contactor so that the
contactor is open. With contactor thermostat, supra energy counter. The temperature
gradually falls, the pressure on the thermostat sensor returns down so that the contactor is
closed again. Every now and then. And Waterbath is a heater that uses a dry heater. This
heater is controlled using a thermostat.
Part Of the Waterbath
1. ON/OFF switch, is used to turn on and turn off waterbath
2. Temperature control knob, is used to set temperature that we want
3. Thermometer, is used to show the real temperature on waterbath
4. Concetric ring cover, is used to cover the inside of waterbath
5. Indicator lamp, it turns on to indicate that the water bath is working
6. Draining valve, is used to drain water from tank of water bath
7. Ventilation, is used to remove the steam
How to use waterbath
1. First of all, make sure the completeness of the waterbath
2. Open the ring cover
3. Fill waterbath with distilled water untill the limit on the waterbath
4. Put test tube and its rack in waterbath
5. Connect waterbath to electric source
6. Press the ON button
7. Set the temperature that we want.
8. Wait a minute, look the thermometer if it has reached the desired temperature
9. If waterbath is working, indicator lamp will light up
10. Wait until finish. Press the OFF button and plug out from electric source
11. Wait a minute until the water temperature in waterbath down
12. Empty the water in the waterbath through draining valve beside the waterbath
13. Clean up the waterbath with clean cloth.
1. Avoid the use of the water bath in environments where there are flammable and
combustible materials. The equipment has components (resistors generating very high
temperatures) which could start an accidental fi re or explosion.
2. Always connect the equipment to an electrical outlet with a ground pole to protect the user
and the equipment from electrical discharges. The electrical connection must comply with the
required norms of the country and the laboratory.
3. Use the waterbath exclusively with non-corrosive or non-flammable liquids.
4. Use personal protective elements when working with the waterbath. The bath has resistors
which can cause burns if inadvertently touched, even a considerable time after turning off the
5. When working with substances that generate vapours, place the waterbath under a
chemical hood or in a well ventilated area.
6. Remember that liquids incubated in the waterbath tank can produce burns if hands are
inadvertently placed inside it.
7. Take into account that the waterbath is designed for use with a liquid inside the tank. If the
inside is dry, the temperature of the tank can become very high. Use the diff using tray for
placing the container inside of the filled tank of the waterbath. This has been designed for
distributing the temperature in a uniform way.
8. Avoid using the water bath if any of its controls is not working, e.g. the temperature or
limit controls.
Warning: Before carrying out any maintenance activity, disconnect the equipment from
the electrical feed outlet.
Waterbaths are equipment whose maintenance is simple. The recommended routines mainly
focus on the cleaning of external components. The most common routines are featured next.
Frequency: Monthly
1. Turn off and disconnect the equipment. Wait until it cools to avoid the risk of burns and
2. Remove the fl uid used for heating. If it is water, it can be poured through a siphon. If it is
oil; collect into a container with an adequate capacity.
3. Remove the thermal diff usion grid located at the bottom of the tank.
4. Disassemble the circulator and clean to remove scale and potential algae present.
5. Clean the interior of the tank with a mild detergent. If there is any indication of corrosion,
use substances for cleaning stainless steel. Rub lightly with synthetic sponges or equivalent.
Avoid using steel wool to remove rust stains as these leave particles of steel which could
accelerate corrosion.
6. Avoid bending or striking the temperature control capillary tube generally located at the
bottom of the tank.
7. Clean the exterior and interior of the water bath with clean water.

1. Waterbath doesn’t need ON/OFF button to turn it on/off.
2. Waterbath is used to heat samples in water at a constant temperature over a long period
of time.
3. Waterbaths are normally used temperature range between room temperature and 60
4. Waterbath uses tap water to fill the water tank.
5. Indicator lamp in waterbath turns on to indicate that there is an error occured to the