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a diagnosis of anemia

Anemia is a condition that occurs when the body's cells, red blood cells (erythrocytes)
and / or Hemoglobin (Hb) in the blood of healthy under normal values (anemia). Hemoglobin
is the main part of the red blood cells that binds oxygen to function.

The characteristics or symptoms of anemia (Less Blood)


A person with anemia usually have characteristics often look very pale and may also
experience other symptoms of anemia, such as:

Weak and rapid exhaustion sleepy tired Easy Headaches Cold hands and feet
Fainting Dizziness, especially when the person stands Shortness of breath, especially
on exertion rapid heartbeat or palpitations, especially on exertion. Chest pain
Decreased concentration and memory

to diagnose the laboratory examination

Tools and materials used :

1. Complete hemocytometer, consisting of:

pipette erythrocytes

IN Calculate Room and Deck glass

The vacuum hose

2. Microscope

reagents:

1. Solution Hayem

2. Venous blood anti-coagulant EDTA +

a. Pipette filling erythrocytes:

1. Suction blood up to the mark of 0.5; clean the outside of the pipette.

2. With the same pipette solution Hayem suck up to the mark 101. Take care to avoid
air bubbles.

3. Remove the rubber suction pipette tip and then close second with two fingertips.

4. Blend for 15-30 seconds (± 80 TIMES).


5. If not immediately counted place the pipette in a horizontal position.

b. Rooms fill Count


1.Kamar Calculate and Deck Glass in a clean state.

2. Place the counting chamber in a horizontal state then wet the two berms with water.
Put a glass deck above it to stick.

3. Shake the pipette before, lest there spilled liquids.

4. Discard the first and then 3-4 drops of subsequent drops included in the room count.

5. Enter the counting chamber by means of touching the pipette tip at an angle of 30 °
on the surface of the counting chamber. Then by itself would count the rooms filled
with liquid.

6. Let the counting chamber for 2-3 minutes, if not immediately counted store room
count in a petri dish by wet cotton.

c. Calculating the amount of erythrocytes

1.rooms count on a table and then use the microscope objective lens 40X, observe the
spread of cells evenly and then count the number of erythrocytes in 5 fields being in
the middle.
WORK SCHEME:

1. Blood pipette to a scale of 0.5

2. The pipette solution Hayem to mark 101


3. Removable Rubber Vacuum and homogenized

(Do not Forget dumped 3-4 drops of the liquid before being put into Room Count)

4. The liquid that has been put in the room homogeneous Calculate Improved Neubauer
5. Observed spread of erythrocytes with a 10x objective lens and the calculated amount ertrosit at
40x objective lens.

Sightings erythrocytes Under Microscope

•CALCULATION

1.Faktor blood dilution 200x

2.Volume the field amid = 1/5 x 1/5 x 1/10 = 1/250


3.Misalkan obtained N cells on the field being amidst so the number of erythrocytes
per mL of blood

= N x 5x 1 / 250X 1/200
= N x 5/50000

= N x 1/10000

= N x 10000

NORMAL VALUE:
• Male - Male: 4.5-5.5 (million cells / ml blood)
• Women: 4.0-5.0 (million cells / ml blood)
• Infants (mature, cord blood): 4.0-6.0 (million cells / ml blood)
• Infants 3 months: 3.2 to 4.8 (million cells / ml blood)
• Children 1 year: from 3.6 to 5.2 (million cells / ml blood)
• Children 3-6 years: 4.1 to 5.5 (million cells / ml blood)

• Children 10-12 years: 4.0 to 5.4 (million cells / ml blood)

if it has been performed a series of laboratory tests then if the calculation


result is less than the normal then it could be one for diagnosing anemia.

Nama: Muhtar sidi


No : 25
Kelas : D3 ankes