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Antimicrobial activity of flower extract, Hibiscus rosa sinensis

Baronda, P.C.D.1., Ecito, K.A.1, Llones, R.V.1, Udtujan, M.1
Leyte Normal University
Tacloban City

Keywords: antimicrobial, flower extract, Hibiscus rosa-sinensis, Agar disc method

Introduction:

Disease causing bacteria spread in seconds that usually cause infections from organism to

organism, especially if unsuspected, leading to fatality of the infected organism if left untreated.

Antibiotics is known as a treatment for these infections, whereof, as time passes these antibiotics

also loses their effectiveness because of these diseases adapts and become resistant to antibiotics.

(Kowti, et al., 2010)

The use of medicinal plants is widely used in traditional and modern medicine. In ancient

times, plants are used for treatment or remedy to almost all of the diseases. (Selvi, Isaivani, &

Karpagam, 2012) Despite the remarkable progress in synthetic organic medicinal products of the

twentieth century, over 25% of prescribed medicines in industrialized countries are derived

directly or indirectly from plants (Newman et al., 2000). Undoubtedly, medicinal plants are the

prime source of drugs in both developing and developed nations, as drugs or herbal extracts for

various chemotherapeutic purposes. (Kowti, et al., 2010) Medicinal plants possess many

potentially valuable therapeutic agents that provide an impetus for further search (Recio and Rios,

1989). Testing micro-organisms for their susceptibility to antimicrobials is a commonplace

laboratory procedure that serves as an important aid to chemotherapeutic intervention in cases of

Review of Related Literature: Hibiscus is a genus of plants that includes over 200 species. 2012). (Alopecia Areata.infection. Anderson. Hibiscus rosa-sinesis Linn. are largely synonymous with tropical environments throughout the world. & Daramolab. (Johnson). the herbal products in the market intended for hair growth include the extract of various parts of Hibiscus rosa sinesis Adhirajan et al. flowers and . available in a wide range of fl ower colors. Moo-Younga. Various parts of this plant.coli and S. It is well accepted that the leaves and flowers of this plant have many medicinal uses which has made this plant a precious one (Upadhyay and Upadhyay. (Irobia. Hibiscus rosa-sinensis. still used plants for their remedies. Today. 2011). One disease example is the alopecia which is a type of hair loss that occurs when your immune system mistakenly attacks hair follicles.aureus. reported that the leaf extract of Hibiscus rosa-sinesis has a potential effect on maintaining the hair growth in in-vivo and in-vitro methods. people who cannot afford medicines. It’s common name is Tropical Hibiscus or Chinese Hibiscus. 1994). 2014) This study aims is to test antimicrobial properties Hibiscus rosa-sinensis extracts to the bacteria E. The tropical hibiscus. It is well accepted that the leaves and flowers of Hibiscus rosa-sinesis have hair growth promoting and antigreying properties. Moreover in India. Hibiscus rosa-sinensis is a tropical plant and shrub that is about 10 feet tall (Gilman. Throughout the tropics this plant is found commonly and it is planted as a house plant throughout the world (Kumar and Singh. like leaves. is best known for its showy fl owers and glossy leaves. (Malvaceae) is a glabrous shrub widely cultivated in the tropics. 1999) under the family Malvaceae. These plants.

oral contraceptive. Sowmia. 2013). sterols and the lack of tannins and Saponins. aphrodisiac. (Upadhyay & Upadhyay. emollient. constipating. Flower petals has a variety of uses. It was also also investigated for their fatty alcohol. glycosides. taraxeryl acetate and four uncharacterized compounds which included an alkaloid and three sterols has been reported in the leaves. refrigerant. aperients. & Jadhav. resin. emmenagogue and used for treating aloparia. to shine shoes in india. leaves and stembark are used for abortion. menorrhagic. & Jadhav. demulcent. . (Pekamwar. fatty materials. red flower for tea. & Joona. burning sensation in the body. The flower extract is used internally in the treatment of excessive and painful menstruation. 2014. Kalyankar. fatty acids and hydrocarbon content. anodyne and laxative. 2013. skin disease and antiseptic for boils and ulcer. 2011) Different extract of Hibiscus rosa-sinensis plant revealed the presence of alkaloids. The staminal column is diuretic used for kidney trouble. laxative. Dhanya. The leaves of H. etc. The essential oil in the seeds of H. Kalyankar.. 2013). (Essiett & Iwok. Isolation of β-sitosterol.roots. Pekamwar. reducing sugars. Divya. made into a kind of pickle or used as a purple dye for colouring foods such as preserved fruit and cooked vegetables and is known as astringent. 2014). rosa-sinensis has a strong antispasmodic effect and has been successfully used to ease the pain for intestine pile or kidney colic. (Essiett & Iwok. salad in Pacific Island. rosa-sinensis are emollient. diabetes and menstrual disorders. fatigue. hypoglycemic. have been known to possess medicinal properties like aphrodisiac.

dried. & Karpagam. 2012. After solidifying. 74 Lower Nulatula. Then. 50. & Ibnsouda. label the plates into 5 for control. sterilized and filled with nutrient agar medium with uniform thickness. 50g of the powder was filled in the thimble and extracted successively with petroleum ether. Balouiri.Materials and methods Plant material The Hibiscus rosa-sinensis Flowers will be collected at Brgy. Sadiki. Microbial cultures Antimicrobial activity of flowers extract will be investigated against pure culture bacteria Escherichia coli and Streptococcus aureus. chloroform and methanol using a Soxhlet extractor for 48 h. are placed on the agar surface. 75 and 100 μg/ml concentration of the flower extract. All the extracts were concentrated using rotary evaporator. 2016) Petri dishes were cleaned. then inoculate and streak evenly the plates with the bacterial strains such Escherichia coli and Streptococcus aureus. 25. filter paper discs (about 6 mm in diameter). Extraction of crude drug The plant materials will be shade dried and then powdered with the help of a blender. All the Petri dishes were incubated at 37ºC for 24 . Isaivani. Antimicrobial activity The antimicrobial assay was performed by agar disc diffusion method (Selvi. containing the flower extract at a desired concentration. Tacloban City.

The assessment of antibacterial activity was based on the measurement of diameter of inhibition zone formed and the zone was measured. coli S. SOLVENTS Microorganism Methanol Chloroform Petroleum ether 100 75 50 25 C 100 75 50 25 C 100 75 50 25 C ZONE OF INHIBITION (mm) E.hrs. aureus .

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