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Grace Valchar

AP Biology
Mrs. Hawley
May 9, 2018

AP Biology Transpiration Lab

1. Transpiration is the process by which plants absorb water through their roots and utilize it
for photosynthesis then emit water vapor through their stomata through the process of
aerobic respiration.
2. Water is taken into the plant through the roots and the release of water vapor happens
with the “pores” of the leaf called the stomata.
3. Factors that could affect the rate of transpiration could include the amount of rainfall,
ATP, and Carbon Dioxide. In other words, the amount of transpiration depends on the
amount of products for aerobic respiration there are.
1. The variable that we tested was wind.
2. Our hypothesis was: “If we set a plant in front of a fan that blows 24 hours straight for 5
days, the weight of the plant will drop by 25% from the original weight.”
3. Procedure:
a. Get two equal sized plants, cut off buds or flowers.
b. Put each into its own plastic bag, tie it off, label one as control and the other as
variable (wind).
c. Weight each separately and record the mass in grams.
d. Place the variable in front of a fan that blows constantly, place control in a
location that will get enough sunlight.
e. Weight each again every twenty-four hours for 5 days while recording the results.
Data: Commented [1]: data for other variables?

Weight in Grams

Days Control Variable (Wind)

1 90.2 101.5

2 80.1 90.1

3 70.1 80.1

4 62 70.9

5 54.7 64.1
Cumulative % Change

Days Control Variable (Wind)

1 0% 0%

2 11% 11%

3 22% 21%

4 31% 30%

5 39% 37%

The independent variable is time, or the amount of days that the experiment lasted, and the
dependent variable was the change in weight the was the result of the plant being left either in a
neutral area or in front of a fan.

1. Many factors can help the movement of water through a plant and into the air. These
include osmosis, root pressure, adhesion and cohesion. Water potential can be decreased
in plants due to the upward transpirational pull on the fluid in the xylem, causing negative
pressure and for the water to move into the xylem.
2. The advantage of closing stomata is the conservation of water when there is low or no
rainfall. But the downside is that photosynthesis cannot occur when the stomata are
closed because carbon dioxide cannot enter the leaf.
3. Plants could adapt to having smaller leaves in order to reduce water loss. They could also
have fewer stomata, or no stomata on their leaves, just on the bottom epidermis.
4. Sources of error that could have occured in this lab include the weighing system and the
calculations for the percent change. There is always a change that quantitative data is has
a mistake from calculating or from keeping a consistent manner of weighing or
measuring a plant.