Professional Documents
Culture Documents
O Cancer
One C Center’s
C Keys to S
Seeing the
BIG Picture
Disclosure
• Ms Burch is receiving an honorarium for
her participation in this educational event
1
Learning Objectives
• Give 3 examples of how Complete Blood
Count results are used in the care of
oncology patients.
• Give 2 examples of how practiced Scatter-
Plot interpretation improves oncology
patient care while providing timely and
reliable results to the clinician.
• Identify 2 keys to begin more advanced
hematology Scatter-Plot interpretation.
2
Doing more with less…
• Lab Automation
• LIS Auto-Verification
• POCT
• Cross-Trainingg
• Thorough information
interpretation?
Playing it safe!
• Hold the results – wait for slide
confirmation
• “Catch the false negatives”
• Manufacturer says to
• % or absolute cut off = manual diff
• Blasts counted as Monos…
• Manual WBC’s and PLT’s
3
Routine CBC’s
• Infections
I f ti – viral
i l or bacterial
b t i l
• Anemia – inherited or acquired
(acute or chronic)
• Thrombocytopenia
Th b t i
• Leukemias
CBC’s in Oncology
• Chemotherapy and Radiation Treatments act
b iinterfering
by t f i withith cell
ll division:
di i i
• Neutropenia
• Thrombocytopenia
• Anemia
• Treatments to counteract above side effects:
• Neupogen/Neulasta
• Plt Transfusion
• Pro-Crit/Aranesp, RBC Transfusion
4
Oncology…cont’d
• Recurrence of leukemia
• NRBC’s – Response to growth factor
or tumor invasion of bone marrow?
• Accurate WBC, ANC, Hgb and PLT
results
l needed
d d QUICKLY for f
treatment decisions!!
Fluorescent NRBC
5
Removal NRBC influence from WBC and DIFF count
Total WBC Count
WBC NRBC
-
Minus Correct WBC#
NRBC
DIFF - Correct
Minus
Differentials
+ +
6
Platelet
Clumps
Plt
Clumps
Ghost
HPC
IMI Channel
RF
IMI Scattergram
RF
PLT
CLUMPS
Immature Gran
Gh t
Ghost
Ghost
Blasts
HPC
DC
7
Fluorescent Optical Plt
RBC Frags
Lg Plts
8
Extended Diff – IG Example
9
Using the IMI Channel Example
Monos
????
10
Using the IMI Channel
Patient’s Manual Diff
Neu % = 16
L
Lymphh% = 39
Atyp Lym% = 10
Mono % =0
Myelo % =1
Promeylo % =1
Blasts % = 33
NRBC’s = 5/100 WBC’s
Auer Rods Seen
11
Using the IMI Channel - 2
Hyper-segmentation of
Neutrophils
(No plt clumps)
12
Using the IMI Channel - 2
Hypersegmented Neutrophils
= more nucleic acid = more
fluorescence.
13
Plt Clumps – Example continued
14
NRBC’s – Example 1
15
NRBC’s – Example 2
16
NRBC’s – Example 2 continued
Patient’s Manual Diff
Neu % = 15
Bands % = 1
Lymph % = 40
Mono % = 1
Atyp Lym% = 43
NRBC’s = 72/100 WBC’s
Micromegakaryocytes = 19/100 WBC’s
Micromegakaryocyte nuclear fragments
15/100 WBC
WBC’ss NRBC count higher on manual
Further WBC correction needed, diff because the instrument is
however – initial correction is done counting the MMKC’s as
by the analyzer so the final WBC’s.
correction is not as drastic.
Blast
Micro-Megakaryocyte
Micro-Megakaryocyte
g y y
Abnormal Platelets
17
Plts and RBC Frags Example
18
Plts and RBC Frags Example cont.
RBC Fragments
RBC Fragments
19
Plts and RBC Frags Example cont.
Smear:
Outcome: Optical plt count verified and released. No smear review done.
20
Optical Platelet Example cont.
Outcome: Optical count reported
without smear follow up, patient
treated.
Clean
S
Separation
i
21
Just Plain Interesting…
22
Just Plain Interesting…
23
Compare… Babesia Patient
Time of Dx
Optical Count 86
3 Weeks post
treatment.
PLT-I = 357
PLT-O =351
Optical Count 64
24
Total WBC Case
25
Total WBC Case
WBC/BASO Count
26
Let’s put it all together…
27
Let’s put it all together…cont.
Outcome: All results released and Optical plt count without smear
verification.
Smear review done and showed left shift Myelocytes, 1+ Toxic Gran and
Dohle Bodies.
28
QUESTIONS???
29