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UNIVERSITY OF MUMBAI

Syllabus for the M.Sc. Part - II

Program: M.Sc.
Course: Life Sciences

Specialisation:
Biotechnology
[Sem III and IV]

(Credit Based Semester and Grading System with
effect from the academic year 2013-2014)

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M.Sc. Part – II Life Sciences Syllabus
Restructured for Credit Based and Grading System
To be implemented from the Academic year 2013-2014

SEMESTER III

Course Code UNIT TOPIC HEADINGS Credits L / Week
I Biomathematics
II Research Methodology
PSLSCEBTT301 4
III Biostatistics
IV Population Biostatistics

I History of Biotechnology
II Microbial Biotechnology
PSLSCEBTT302 III Plant and Animal Biotechnology 4
Protein Engineering and GMO
IV
detection methods

Process Biotechnology –
I
Introduction
PSLSCEBTT303 II Upstream processing 4
III Downstream Processing
IV Measuring, Modelling and control

Microbial biotechnology in
I
Industry
Biomass processing and
II
PSLSCEBTT304 bioenergy production 4
III Biotransformations
Products of Primary and
IV
Secondary Metabolism

PSLSCEBTP301 Biomathematics and Biostatistics 2
PSLSCEBTP302 Biotechnology and Genetic Engineering 2
PSLSCEBTP303 Dissertation on Literature Review 2
PSLSCEBTP304 Biotechnology in Industry 2

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SEMESTER IV

Course Code UNIT TOPIC HEADINGS Credits L / Week
I Bioinformatics – I
II Bioinformatics – II
PSLSCEBTT401 4
III Intellectual Property Rights
IV Bioethics

Introduction and therapeutic
I
bioproducts
II Vaccines, antibodies, peptibodies
PSLSCEBTT402 4
Gene therapy, Antisense therapy,
III
Diagnostics and genetic testing
IV Applied Medical Biotechnology

I Water Pollution Control
Metal pollution control and soil
II
decontamination
PSLSCEBTT403 4
Soil and gas waste treatment and
III
bioconservation
IV Agricultural Biotechnology

I Marine biotechnology
Bionanotechnology, biomimetics
II
and drug delivery
PSLSCEBTT404 III Other applications 4
Biotechnology – Biosafety
IV assessment, legal, economics and
ethical issues

PSLSCEBTP401 Bioinformatics 2
PSLSCEBTP402 Medical Biotechnology 2
Environmental Biotechnology and
PSLSCEBTP403 2
Agricultural biotechnology
PSLSCEBTP404 Dissertation on Project 2

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Measures of central tendency (grouped and ungrouped data). Skewness and kurtosis  Population parameters and sample statistics. Differentiation (including differentiability). graphical presentation of data: collection and tabulation and graphical representation of data. census and sampling surveys. Methods of data collection. confidence intervals for population mean & proportion. motivation in research. Rank of Matrices by Diagonalisation method Limit and derivatives.  Research Process: Steps of research process. Standard error.  Differential equations --homogeneous and Linear ODE’s and its simple applications to biological problems Unit II: Research Methodology (15L)  Meaning of Research. sampling techniques: simple random sampling. Successive Differentiation and their application in biological research. median. stratified random sampling. Objectives of research. Applied. Central tendency measures: Arithmetic mean. Sampling. frequency distribution  Practice of statistical methods in biological research.Sc. Fundamental.  Research Approaches.  Data. dispersion and its measures: variance and standard deviation. Sample size determination. Plan for data collection. Criteria of Good Research. Research Methods vs.Descriptive. Plan for data processing and analysis. coefficient of variation. correlation coefficient and 4 . Regression and correlation and its application in biology. Quantitative. Empirical and Other Types of Research.  Ethical considerations during research Unit : III Biostatistics (15L)  Basics: Introduction. systematic sampling. applications and uses of statistics. M. Part – II Life Sciences Syllabus Restructured for Credit Based and Grading System To be implemented from the Academic year 2013-2014 SEMESTER III DETAILED SYLLABUS Course Code Title Credits Biomathematics / Research methodology / Biostatistics PSLSCEBTT301 4 (60L) Unit I: Biomathematics (15L)  Matrices. types of correlation. scope. Application of integration to find area and application in biological research. Conceptual. Integration – Definite and Indefinite. Estimators of population mean & proportion (without proof). Analytical. Methodology. Qualitative. samples and populations.  Types of research .

5 . variance. Formation of frequency distribution and calculation of descriptive measures – mean. median. ANOVA – one way and two way classification.  Analysis of variance (ANOVA). mode. scatter diagram. coefficients and tests of significance 5. Random variable and its distribution. Difference between parametric and non-parametric statistics. Practicals: PSLSCEBTP301 Biomathematics and Biostatistics (60L) 2 04 1. t-test. 2. Probability distributions – Binomial. Devise a research methodology for the project to be undertaken as the dissertation 7. Non parametric tests: Sign test and Run test. Estimation of genetic components and heritability from ANOVA data 6. X2 test and F test. Calculation of correlation coefficient and regression. two-way ANOVA Basic introduction to Multivariate statistics. Large and small sample tests for sample mean and proportion 4. standard deviation and standard error from a given data 3. Poisson and Normal. Non Parametric tests: Sums on Sign test and Run test. Solve sums on derivation and integration related biological data. one-way ANOVA. Tukey’s post hoc test. Theories of Probability – addition and multiplication theorems. Unit : IV Population Biostatistics (15L)  Concept of probability.  Test of hypothesis: Z-test.

Yeast Selectable Markers and Vector Systems. present development . development of rural and urban societies. selection markers. cerevisiae .Introducing DNA into Yeast. Yeast and filamentous fungi. Using Yeast to Analyse Genomes. Gram positive bacteria.  Biotechnology and human development. Detecting Expression of Foreign Genes. GM foods. Course Code Title Credits Biotechnology and Genetic Engineering (60L) PSLSCEBTT302 4 Unit I: History of Biotechnology (15L)  Biotechnology. promoters. medical biotechnology. Genes and Protein-Protein Interactions . polysaccharides from basidiomycetes for 6 . Industrial biotechnology. Introduction. Principles of genetic engineering for E. strategies for efficient production. medicinal mushrooms as neoplastic agents. Gene Knockouts.The Source of Heterologous DNA. coli.old and modern concepts . Trends for future developments: medical. Control of Gene Expression – Prokaryotes and Eukaryotes. role of glycosylation. Heterologous Protein Production . Gene Manipulation in S. overproduction of foldases and chaperones. applications - pharmaceutically important secondary metabolites. signal sequences. coli. agricultural biotechnology. The Expression of Eukaryotic Genes in Bacteria – Introns.Optimizing Expression in E. Future Prospects  Yeast Cloning and Biotechnology – Introduction. Unit II: Microbial Biotechnology (15L)  Genetic engineering of Microorganisms: Classification of microorganisms as per rDNA regulations. starvation). humanization of yeast and filamentous fungi. translational regulation of protein production. Promoters.Maximizing Expression of Foreign DNA . heterologous and homologous gene expression. The Level of Heterologous mRNA Present in the Cell. Expression of Native Proteins. Strategies of strain improvement – random and directed mutagenesis. social aspects (health. historical development. marine biotechnology. Future Prospects  Filamentous fungi –Host strains. use of tags and cleavage. Novel Reporter Systems. gene targeting  The Expression of Foreign DNA in Bacteria – Introduction. directed evolution and in silico methods – engineering of synthetic pathways. commercially used yeast strains and their expression systems. biotechnology and corporate world. gene fusion approach. fundamentals in biotechnology. Gram Negative Bacteria. transformation strategies. health and survival. environmental biotechnology and. Ribosome Binding Site. The Amount of Protein Produced and The Nature of the Required Product. Alternative Host Organisms. poverty.YAC Technology. terminators. Expression of Foreign DNA as Fusion Proteins.

Transgenic Technologies – Selectable Marker and Reporter Genes. Regeneration of plants.Transgenic Mice. The Production of Transgenic Animals by Microinjection . regulation and nutritional requirements.Prolonging Shelf Life. Agrobacterium-mediated Transformation (including the details of the Ti plasmid and its transfer into plant cells). Engineering strategies for crop resistance Herbicides. algae. Nutritional and Technological Properties: Proteins. Production of secondary metabolites by plant suspension cultures.  Applications of Transgenic Technologies:. Manipulating Quality . Analysis and detection. Introduction. introducing novel horticultural traits and manipulating Male Sterility. Tools. R-avr/ plant natural resistance genes). Hairy root cultures. Animal cell preservation Transgenic animals: Transgenesis. Cell suspension culture development: methodology. Insects. Choice of Animal. Transgenic Plants as Bioreactors: Biopharming and Neutraceuticals .. Molecular Evolution – modifying activity.Sequence Identification. (Coat protein mediated resistance. Plant growth regulators and elicitors. Oils. Nematode Pathogens and Abiotic Stresses. DNA Fingerprinting. Protein Structures . Expression. microalgae and protozoa Unit : III Plant and Animal Biotechnology (15L)  Genetic engineering of Plants: Totipotency. Embryo culture. Unit : IV Protein Engineering and GMO detection methods (15L)  Protein Engineering – Introduction. Metabolism.Site-directed Mutagenesis Methods. chloroplast transformation and Particle Bombardment. nutrient optimization Plant products of industrial importance.Edible Vaccines. Hybridoma technology. increasing stability. Plant Viruses. de novoSequence Design. Manipulation of Metabolic Partitioning (advantages of chloroplast / plastid transformation) Production of Plant Polymers and Biodegradable Plastics. 7 . Cloning in animals. Plant Biotechnology in Forestry. Applications of Molecular Biology to Speed up the Processes of Crop Improvement. Kinetics of cell growth and product formation. Manipulation of Starch and Fructans. Non-PCR Methods and PCR-based Methods. Transgenic Plants: Introduction. cofactor requirement. Variety Identification. pH and temperature optima. substrate specificity. movement proteins.cyanobacteria. Production of antibodies and other pharmaceutically important molecules in plants.  Mass cultivation of animal cell cultures. Molecular Markers. Live-stockimprovement. kinetics of growth and production formation. Sequence Modification . Sequence Determination and Modelling. immunostimulating and anticancerous activity  Special microorganisims of biotechnological interest.

Domain Shuffling (Linking. toxicological studies. Fused Cytokines and Fusions to Stabilize DimericProteins. Isolation of plasmid from bacteria. Preparation and regeneration of fungal (T. Analysis of copy number of amylase gene 7. Construction of amylase expression cassette and expression in E. biochemical and molecular. Detection and estimation of gene copy number by Real Time PCR 8 . 3. Effect of an elicitor on the production of the plant secondary metabolite 11. reesei) protoplast 9. Whole Protein Shuffling. detection of chromosomal changes. Secondary metabolite production using plant tissue culture (dye/ drug/ alkaloid etc. future perspectives  Applications . Transformation in bacteria.Point Mutations: Betaseron/Betaferon (Interferon /3. applications. Slide culture of filamentous fungi 8. Swapping and Deleting) Linking – DomainFusions for Cell Targeting.16). residual DNA analysis. Transformation of plant tissues using Agrobacterium sp. product analysis – microbial. 4. Hormone Agonists and Substitution of Binding Specificities. coli 5. de novoDesign. Swapping Protein Domains –Chimaeric Mouse-Human Antibodies and Polyketide Synthases (PJCSs). future  Detection and analysis of GMOs and GMO products: modified gene copy number determination.) 10. Humalog (Lispro Insulin) and Novel Vaccine Adjuvants. 2. RAPD analysis (e. Deleting Domains.g. Protein-Ligand Interactions -Enzyme Modifications. toxicological evaluation PSLSCEBTP302 Biotechnology and Genetic Engineering (60L) 2 04 1. using primers for matK or rbcL for Plants/ or similar for animals) 12.

Transformation Processes. large scale fermentation. Applications of Fermentation . Fed-batch cultivation system. nutrition. inoculums cascading system. such as baker’s yeast. citric acid.principles. cell aggregation/flocculation. growth measurements. The microbial biochemistry concept  Isolation. recycling cultivation system. Media for microbial fermentations. cell culture bioreactors. The Fermentation Process . kinetic and process rate. storage of culture and culture collection resources and services.description and modeling. general features.The Mode of Operation of Fermentation Processes. waste treatment  Microbial cell cultivation systems – Introduction. types – submerged and solid state. product recovery. lab scale fermentation. immobilized bioreactor design and biosensors Unit II: Upstream Processing (15L)  Fermentation Technology – Introduction. growth curve and optimization of nutritional and physicochemical factors. Batch cultivation system. passive immobilization. Recombinant Products. media for cell culture. covalent coupling. exo-polysacharides. reactor design. importance of inoculums. Strategies for fermentation with recombinant microorganisms. The Genetic Improvement ofProduct Formation – Mutation. stock culture maintenance.  Immobilized cells/enzyme systems – alginate. ion exchange resin. 9 . Production and purification of recombinant proteins on a large scale. oxygen transfer and mixing – scale up implications. polyurethane foam. stoichiometry of the process. bioreactor design and application of SSC. Recombination. Immobilization of enzymes and cells and their application for bioconversion processes. Conclusions  Bioreators. Chromatographic and membrane based bioseparation methods. microbial basis of processes. Anaerobic fermentations. biochemical engineering concept – identification of main products and substrates. ethanol. Microbial Growth. amino acids. continuous growth cultivation system.. purification and bioprocess application(s) of industrial enzymes. Microbial Metabolites. solid-state and solid-substrate cultivation system. carrageenan.  Process strategy –primary and secondary metabolites and bioconversions. media and air sterilization. Course Code Title Credits PSLSCEBTT303 Industrial Biotechnology (60L) 4 Unit I: Process Biotechnology – Introduction (15L)  Overview: Bioprocess technology for the production of cell biomass and primary/secondary metabolites. identification and initial selection of microbial strains – culture preservation. Microbial Enzymes. in cell culture reactors. modification of genetic structure to increase product formation. Microbial production. antibiotics and pigments etc.Microbial Biomass. optimal nutritional and physical requirements for growth – microbial nutrition.

fermentation monitoring and control. Cells: Animal Cells. High Performance Chromatographic Techniques.  Immobilization of Biocatalysts – Introduction. Expanded Bed Adsorption. Entrapment. impeller design.Next Generation of Support Material. Equipment for Large-scale Chromatography and Control and Automation. Cellulosome and Multienzyme Complexes and Immobilization Technology. electrokinetic separation of proteins.Ammonium Sulfate. Aeration and agitation. Future Trends Unit : IV Measuring. Immobilization: Choice of Support Material. Batch. Biocatalysts: Enzymes – Specificity and Catalytic Power. Choice of Immobilization Procedure – Adsorption. extraction. product formation. Selection of Matrix. Grinding with Abrasives. mixing. Batch Centrifuges. Scale up. Applications Unit : III Downstream processing (15L)  Protein Extraction and Purification – Introduction. Plant Cells and Microorganisms (Bacteria.Debris Removal.Scale-up and Quality Management. diffusion. Aqueous Two-phase Separation. data analysis for design and control. Biocatalyst Selection. Simple structured models. biotransformations and enzyme reactors  Bioprocess Engineering: Kinetics of microbial growth. Instrumentation in bioreactors. Continuous-flow Centrifugation. Covalent Binding. Affinity Chromatography. lyophilization and spray drying. Scale-up concepts. Basket Centrifuges and Membrane Filtration.  Chromatography . Gel Filtration.  Precipitation . Cell Disruption: Enzymic Methods of Cell Disruption. Sterilization of air and media.  Process Validation and regulatory issues 10 . Ion Exchange Chromatography. Membrane Chromatography.Osmotic Shock. Initial Purification . Physical Methods of Cell Lysis . High Molecular Weight Polymers and Heat Precipitation. Modelling and control (15L)  Selection. Yeast and Filamentous Fungi).  Ultrafiltration. Properties of Immobilized Biocatalysts – Stability and Catalytic Activity. Abzymes. Cross-linking. Encapsulation. Mass transfer in bioreactors. Organic Solvents.Inclusion Bodies and Affinity Tails. Agitation and Heat transfer models  Various issues involved in scale – up fermentations like flow. Perfusion Chromatography. substrate utilization and product formation. Rheology of fermentation fluids. Proteosome. Multienzyme Complexes – PDC. Solid Shear and Liquid Shear. oxygen transfer. in vitro protein refolding. Various types of microbial and enzyme reactors. Hydrophobic Interaction chromatography. Chemical Methods of Cell Lysis – Alkali and Detergents. design of aseptic aerated fermentors. Design of fermentation media. Operation and Control of Bioreactors Aeration. fed-batch and continuous processes. Ribozymes. Design of Proteins for Purification . Method Selection. Maintenance of Column Packing Materials.

Leather Processing. lysozyme. Vinegar.  Food Fermentations – Baked Goods. biotechnology facility design and process validation. Use of Enzymes in Food Processing. enzymes used in meat industry . baking industry. Vegetable Fermentations. Cheese. dairy industry – enzyme from rennet and rennet substitutes. Dairy Processing. Cellulases. hemicellulasesPectinases – pectin methylesteraes. pectin depolymerises. β-1. enzymatic synthesis of aspartame. Paper & Pulp Processing. Improving the quality of bakery products.lactic acid bacteria. Extraction of olive oil. acid proteinases. immunity and mode of action. requirements and regulatory status of bacteriocins. metalloproteases. Grain Processing. Other. Fermented Feeds and Feed Products. biological methods. enzymes in starch processing and baking industry . treatment of biological waste PSLSCEBTP303 Dissertation in Literature Review (60L) 2 04 Dissertation on Literature Review Course Code Title Credits PSLSCEBTT304 Biotechnology in Industry (60L) 4 Unit I: Microbial biotechnology in Industry (15L)  Application of enzymes in industrial processing. Olive Fermentations. production of aroma and texture. hemicellulases. Fruit Processing. chemical preservatives and their safety concerns. classification. vegetable and fish products. laccases  Food biotechnology – Role of Cellulases. 4-galacturonidase in Baking. Lipases – pancreatic lipases and microbial lipases. chiral synthesisof enantiomerically pure compounds. LAB bacteriocins. glucose oxidase. catalase. glucose isomerise. Fermented Feeds. factors affecting its action. meat. characterization. regulation of biosynthesis.laundry. Infusion of pectinases and b-glucosidases to alter the sensory properties of fruits and vegetables Production of fruit nectars and purees. mode of action and 11 .baking. cocoa Fermentation.syrup and sweetener. Fermented Dairy Products. sulfhydral oxidase. endolysins – structure. proteases – serine proteases.  Analytical protein chemistry. Indigenous fermented foods. Brewing. Wine and Brandy  Food Biopreservation – Microbial Ecology of Spoilage and Pathogenic Flora Associated to Fruits and Vegetables. hurdle technology and application of LAB bacteriocin. Textile Processing. Cereal Extraction. glucose isomerisation. applications in dairy. Protein Processing & Flavours. fermentations. role of cellulases. hemicellulases and pectinases in beer and wine biotechnology – Brewing. biosynthesis. Extraction and clarification of fruit and vegetable juices.

. Protein .Catalytic antibodies. fungi. phytosterols. protein enriched whey. algae Production of microbial biomass as enrichment for animal feed: protein enriched starch. Formulation of functional foods containing nutraceutical.  Future of Biotransformations . Risk Assessment. cassava. and Challenge Tests Unit II: Biomass processing and bioenergy production (15L)  Production and processing of microbial biomass: bacteria. Esterases of industrial or pharmaceutical importance (eg. silage.  Nutraceuticals: lycopene. Starch . microbiology. Biotechnological Production of Natural Aroma Chemicals by Fermentation Processes. Regioselective Oxidation of Aminosorbitol with Gluconobacter oxydans.grain. Characteristics of each class of enzyme with a general characteristic reaction and importance in industry. Processing of simple polymer: Enzyme production. Silage: ensiling process. Artificial Peptides in Stereoselective Synthesis 12 . applications. conversion of lignocelluloses into feed using cellulases and hemicellulases of white‐rot fungi.analytical. in the form of gas– hydrogen and methane (biogas). Transferases. stability and labeling issues. enetioselective hydrolysis of racemic esters). glucosamine. quality. Unit : III Biotransformations (15L)  Biotransformation: Introduction and advantages. Tools for Safety Control: HACCP.Industrial Biotransformations. Hydrolases.  Hydrolases –Types of hydrolases and their characteristic reactions. Composting: physical and chemical factors. Sugar – sugarcane. Fatty acid –oil palm. additives. bacteriophages .microflora.  Engineering Microbial Pathways for Amino Acid Production.fish processing industries. isoflavonoids.current bacteriophage based food applications. from crop residues and by products. Lyases. Oxidoreductases. yeast. Synthetic Enzymes. health risk from pathogens. potato and sagopalm. Synthetic Applications of Enzyme-Catalyzed Reactions. Classification of enzymes: An overview (EC number. Biotransformations with Lipases. Predictive Microbiology.  Biorefinery – Biogas.  Oxidoreducatases: Dehydrogenases .Introduction. biofuel from phytoplankton. A Key Reaction in the Industrial Synthesis of 1-Deoxynojirimycin. in Tropical areas. Importance of hydrolases in industry. biofuel in form of liquid– ethanol and diesel. Biofertilizer and Food through aquaculture and algae production Bioenergy: Biofuels . Isomerases and Ligases). prebiotics and probiotics. odour sources.

Microbial Production Lactic Acid. cholesterol inhibitors. Unit : IV Products of Primary and Secondary Metabolism (15L)  Raw Materials and Raw Material Strategies . Extracellular Polysaccharides: dextran. antihelmintic antibiotics. anti-diabetic agents. Peptide antibiotics. Types of products that could be obtained using these raw materials.  Products from Basidiomycetes. approaches to improvement of microbial polysaccharides production. Fermentation of wheat bran.Sugar-Based Raw Materials for Fermentation Applications (Sugarcane).  Products of Primary Metabolism. Novel Receptor-Active Compounds of microbial origin. b. Ethanol - Potential Source of Energy and Chemical Products. Microencapsulation based cell therapy. Microbial Lipids. antitumor agents. Medicinal Mushrooms (antitumor polysaccharides) Secondary products from filamentous fungi: antibacterials. antivirals. terpenoid flavor and fragrance compounds. insecticidal. Nucleotides and Related Compounds. Technical Production and Use of Amino Acids. alginate. Microbial Siderophores. Starch-Based Raw Materials for Fermentation Applications (Wheat starch). polyketidesysnthesis. Lantibiotics. Antibiotics: Biology and pharmacological importance of β-Lactam Antibiotic Biosynthesis. xanthan gum. Formation of sugars c. Analysis of: a. Purification and Precipitation of these enzymes and enzyme activity in these fractions. 2-D gel electrophoresis of precipitated protein 13 . 2. crude enzyme formation (cellulase activity / other enzymes).  Production of Biochemicals -production of vitamins (Vitamin C). Aminoglycosides. anticancer. Cyclosporins: pharmacology and biology and clinical applications. PSLSCEBTP304 Biotechnology in Industry (60L) 2 04 1. degradable resins. d. Biosurfactants  Products of Secondary Metabolism: General Aspects of Secondary Metabolism. Glycopeptide Antibiotics. PHB. cellulose and wood shaving powder by Trichoderma or Aspergillus niger or suitable lignin and cellulose degrading fungi. other bioactive compounds. Ethanol: Classical Methods. Polyacrylamide gel electrophoresis of the precipitated enzyme e. (Dalbaheptides).

Neighbor-Joining Method. Composite. Detection of GMO food 7. 3. Consensus. Biotransformation ( a sample reaction: substrate to product) 6. Objective. The Dot Plot. Internet sources for Bioinformatics. DDBJ. and Secondary) (c) Specialized Genome databases: (SGD. Starch based gum from Tamarind seeds and their advantage over others. Pairwise sequence alignment: NEEDLEMAN and Wunsch algorithm. Acacia gum. (b) Protein databases (Primary. Smith and Waterman algorithm. rooted and un- rooted trees. Analysis of Free Amino Acids 4. Terminology of tree-reconstruction.  Phylogenetic Analysis: Phylogenetic-trees. Properties of different gums like Sterculia gum. Unit II: Bioinformatics – II (15L)  Protein structure analysis and prediction: Identification/assignment of secondary structural elements from the knowledge of 3-D structure of macromolecule using DSSP and STRIDE 14 . SEMESTER IV DETAILED SYLLABUS Course Code Title Credits PSLSCT401 Bioinformatics and IPR and Bioethics (60L) 4 Unit I: Bioinformatics – I (15L)  Introduction to Bioinformatics: Definition and History of Bioinformatics. and ACeDB) (d) Structure databases (CATH. and PDBsum)  Alignment problem and solutions: Alignment: Basics and techniques.  Biological databases: (a) Nucleic acid databases (NCBI. and EMBL). TIGR. Local alignment and Global alignment. Algorithms /methods of phylogenetic analysis: UPGMA. Production of algal biomass 5. SCOP. Microbial load of canned foods/ preserved food stuff 8. Dynamic Programming Algorithm. Multiple Sequence Alignment (MSA): Definition.

WIPO treaties. Biosensing Unit : III Intellectual Property Rights (15L)  Introduction to IPR. immunology. and ab-initio structure prediction methods. PCT. agriculture.  Patent databases. Patenting rules in different countries Unit : IV Bioethics (15L)  Bioethics: Definition – moral. Indian Patent Act (1970)  Patents: Definition. forestry. Posthumanism and Anti-Posthumanism. values.  Genomics: Basic concepts on identification of disease genes. fold recognition. Conventional. animal welfare & right / animals in research. Beneficience. biotechnology. types of patent application – Ordinary.  Bioethics in healthcare. commercialism in scientific research  Bioethics and cross-cultural bioethics – Autonomy.  Concept of Prior Art. Drug targets. Homology Modeling.  Reasoning behind Acceptance or Rejection of Genetic Manipulation. Madrid agreement. 15 . Novelty. Tertiary (3-D) Structure prediction: Fundamentals of the methods for 3D structure prediction. wildlife conservation and management. modern biology. Basic Approaches to Ethics. identification of SNPs. Computer-Aided Drug Design. methods. threading approaches. SNP database (DbSNP). agriculture. Types of Intellectual property – Patents. ethics and ethics in biology. Copyrights and related rights. scope. role of bioinformatics- OMIM database. Pre-clinical and Clinical Testing  Applications Of Bioinformatics: Pharmaceutical industries. Budapest treaty. litigation. patentable and non-patentable inventions. Animal Rights. Role and importance of ethics in biology. Patent infringement – meaning. Hague agreement. Importance of intellectual property rights in biology and environmental sciences. Environmental ethics. Justice. Prediction of secondary structure: PHD and PSI-PRED methods. and Patent of addition. Lead Identification and Modification.  Drug discovery and Development : Introduction to Drug Design and Development. Time frame and cost. Do No Harm.  GATT and WTO and IPR provisions under TRIPS. Precautions while patenting . SNP arrays. Trademarks.disclosure / non- disclosure. Divisional. Drug Delivery. Rights. Mixed Perception of Benefit & Risk. Concerns about Consuming products of GMOs. Confidentiality. case studies. Traditional vs.

Human Misuse Practicals: PSLSCEBTP401 Bioinformatics (60L) 2 04 1. vitamin. Homology Modeling Using Modeller 10. Case study – Bioethics for GMO 12.  Gene Therapy and DNA/RNA-Targeted. Therapeutics. Regulatory Concerns. TNF.Interference with Nature . Molecular Biology of Disease and in vivo. epinephrine. Biological Databases with Reference to Expasy and NCBI 2. Medical applications in humans and animals. 16 . Future Prospects in Pharmaceutical Research. Pairwise sequence alignment 5. relaxin. erythropoietin. insulin. Structural Biology and Rational Drug Design. steroid hormones. Conclusions  Therapeutic Proteins: In vitro folding of inclusion bodies on an industrial scale. Tertiary Structure Prediction 9. Case study – IPR and India Course Code Title Credits PSLSCEBTT402 Medical Biotechnology (60L) 4 Unit I: Introduction and therapeutic bioproducts (15L)  Molecular. Queries based on Biological databases 3. Chemical Biology and Molecular Diversity. Genomic Protein Targets and Recombinant Therapeutics. Gene Prediction 7. interferons and interleukins. Transgenic Models. Past and Present ‘Bioethical Conflicts’ in Biotechnology. Secondary Structure prediction 8. Monoclonal Antibodies. human hormones – Growth hormones. blood proteins. . Multiple Sequence and Phylogenetic Analysis 6. immune modulators – factors VIII IX. Sequence similarity searching using BLAST 4. Fear of Unknown. Structural and Chemical Biology in Pharmaceutical Research- Introduction. Case study – Various Applications of Bioinformatics 11. somatostatin.

Analysis of Vaccine Antigens . Mucosal Immunity. Improving Attenuation in Vibrio and Improving Stability – Poliovirus.HIV I gp120. Screening Phage Display Libraries of Immunoglobulin Genes. Live Attenuated Vaccines and The Relative Merits of Live versus Killed Vaccines. Anti-idiotypes. Choice of Myeloma Cell-line. Preparation of Myeloma Cell-line and Host Immune Lymphocytes for Fusion. antibodies.The Scale of the Problem. Enhancing Immunogenitity and modifying Immune Responses .Bulk Production and Isolation and Purification. Carriers and Vehicles. ‘Live-dead’ Vaccines. Other Virus Vectors and Recombinant E. Cross-species Vaccination. Analysis and Production: Identification and Cloning of Antigens with Vaccine Potential - DNA/Oligonucleotide Hybridization. Cloning Hybridomas. Screening Hybridoma Culture Supernatants. Isolation and Purification of Monoclonal Antibodies .Adjuvants. Improvement and Generation of New Live Attenuated Vaccines: Improving Current Live Attenuated Vaccines . Preparation of Soluble scFv and Screening Supernatants Containing soluble scFv. Hybridoma Formation by Somatic Cell Fusion. Monoclonal Antibodies in the Diagnosis 17 .Isolation of mRNA for VHand VL and Generation of cDNA. Modulation of Cytokine Profile. Other Approaches to Vaccines: DNA Vaccines (Genetic Immunisation) – cholera Optimizing Responses and RNA Immunisation. Linking of VH and VL to Give scFv. Immunogen and Route of Immunization. Recombinant Live Vectors . Current Vaccination Strategies . Monoclonal Antibodies in Biomedical Research. Attenuated Salmonella Strains as Live Bacterial Vaccines.Identification. Examples of the Preparation of Rat Monoclonal Antibodies Which Have Been Used to Investigate the Structural and Functional Properties of Macromolecules - Rituximab: Clinical Development of the First Therapeutic Antibody for Cancer. Genes and Expression on the Surface of Bacteriophage . coliStrains. mAbs to Growth Factor Receptors and Monoclonal Antibodies for Clinical Application. Recombinant BCG Vaccines.B-cell Epitopes and T-cell Epitopes. PCR Amplification of cDNAs for Antibody VH and VL. Modulation by Antigen Targeting and. tissue plasminogen activator protein and vaccines. Expression cloning and Genomic Sequencing. Peptide Vaccines. The Role of Genetic Engineering in Vaccine .Inactivated Vaccines.Expression of Potential Vaccine antigens. Generation of Subunit Vaccines .New Vaccines for Pseudorabies Virus.Vaccinia Virus Recombinants. Choice of Host for production of Immune B-cells. Insertion of scFv into Phagemid Vector. Modulation of Signalling  Monoclonal Antibodies – Introduction. Carriers. Bulk Production. peptibodies (15L)  Vaccination and Gene Manipulation .Infectious Disease .  Generation of Monclonal Antibodies Using Recombinant Gene Technology: Isolation of Immunoglobulin Variable Region. Antibody Structure. Hybrid Selection and Cell-free Translation. Viral antigens by bacteria Unit II: Vaccines. Expression of scFv on the Surface of Bacteriophage. Preparation of Hybridomas by Somatic Cell Fusion: Principle of the Technology. Poliovirus Chimaeras.

 Antisense therapy. multi-potent adult stem cells. Frontiers of contraceptive research. Therapeutic Applications of Monoclonal Antibodies: A Clinical Overview: Case Studies – Antibodies for Sepsis: Some Lessons Learnt. Diagnostics and (15L) genetic testing. gene transfer or transfection using eggs of cultured stem cells. ovarian cancer and immune thrombocytopenic purpura. Use of Antibodies for Immunopurification. infertility and reproductive vaccines.testicular. and Treatment of Disease. and Fluorescently Labelled DNA Sequencing. ovarian and breast cancer. study of reproductive cancers. ethical issues related to embryo research. Interpretation of the Results. Antisense therapy. applications – pain. and Immunogenicity. triple . DNA Chip Technology. Point Mutations . viral diseases. Oligonucleotide Ligation. Regeneration therapy. Preclinical Testing of Antibodies: Pharmacology. 18 . gene function analysis and in agriculture. embryo culture and embryo transfer technology.Detection of Deletions. first generation antisense drugs. Overview. catalytic antisense RNA. and mechanism of action. Preclinical Testing of Antibodies: Safety Aspects. Duplications and Insertions. pluripotent adult stem cells. Cystic fibrosis gene therapy. Mitochondria1 DNA. PCR Technology. production in E. Gene therapy for inherited immunodeficiency syndromes. and limitations.  Molecular technologies and Diagnosis of Inherited Disease: Introduction. production. transgenic stem cells. MLP and SLP Technology.  Stem cells & therapeutic cloning . HIV-1 gene therapy. development of transgenic animals such as mice. Future . Kinetics. peptide-Fc fusion. Short Tandem Repeats. coli using recombinant DNA technology. Applications : cancer therapy.Allele-specific Oligonucleotides and Restriction Enzyme Site Analysis. oligodeoxyribonucleotide. strategies.Embryonic stem cells and therapeutic cloning. An Engineered Human Antibody for Chronic Therapy: CDP571. Expansion Mutations.  Gene therapy. Direct Detection of Gene Mutations. Antibody Targeted Chemotherapy. Databases. Biotechnology medicine. cryopreservation of sex gametes and embryos. production. advantages over monoclonal antibodies. Indirect Diagnosis with Linked Genetic Markers.helix forming oligonucleotides (TFOs). viral and non viral Vectors for somatic cell gene therapy. Introduction. sexually transmitted diseases and vaccines.(Paternity and Forensics) Unit : IV Applied Medical Biotechnology (15L)  Reproductive biology: Superovulution. ARMS. Y Chromosome Analysis. second generation antisense drugs. limitations Unit : III Gene therapy. Cancer screening  DNA in Forensic Science: Introduction.Capillary Electrophoresis. ReoPro Clinical Development: A Case Study  Peptibodies – definition.The First PCR-based Forensic System. DNA probes for diagnosis in epidemiology and forensic science.

biological containment. Detection of disease (Thalassemia and breast cancer) mutations by PCR 6. SCID. RIA. immmunophenotyping. pharmacogenomics of cancer syndromes. immunological and molecular methods of detection. epitope mapping and design of synthetic vaccines. Legal issues: legal actions taken by countries for use of the molecular technologies. 4. Detection of a protein/ substance using ELISA. relevance to immune system  Patho-biotechnology: concept. Residual DNA/ antibiotic resistance marker analysis of a recombinant preparation. future prospects. Identification of amplified DNA by sequencing 3. study of mechanism of disease development and therapy.Aggressive. transplantation immunology: HLA typing method using serological and molecular techniques. cardiovascular diseases and smoking and alcoholism. 8. (insulin or any other). and violent behavior. 2. DNA fingerprinting 5. autoimmune diseases and their treatment. Regulation of Human Gene Therapy. regulations of antibodies and recombinant proteins. impulsive. therapeutic.Minimum Inhibitory Concentration of the antibiotic. regulation of gene therapy.  Pharmacogenomic: types of pharmacogenetic knowledge and obstacles. DNA vaccines. Alzheimer’s disease. types: bioengineered.  Regulatory and Social Aspects Regulation of Antibodies and Recombinant Proteins. economic considerations PSLSCEBTP402 Medical Biotechnology (60L) 2 04 1. tumor immunology: basic concepts and immunological approaches to tumor therapy. long QT syndromes. role of metagenomics.  Genes and Human behavior . genetic influences on drug targets involved in pharmacodynamics. ELISA. apoptosis: biochemical. monocytogenes as a vaccine delivery vehicle. L. idiotyping vaccines and T-cell vaccines. transgenic and knock out animal models . application of avidin-biotin interactions. Schizophrenia susceptibility loci  Immunotechnology: Immunoassasys: SRID. Multiplex PCR 7. designer. and flow cytometry: principles and applications. Antibiotic sensitivity assay . Probiotics: essential prerequisites. Synthesis of silver nanoparticles using a biological extract (plant/ microbial) 19 .

Future Aspects .Contaminated Soil Areas. Modeling of Biogas Reactors. Artificial Groundwater Recharge and Bank Filtration. Perspectives of Waste. Possibilities and Potential of Anaerobic Wastewater Treatment Using Anaerobic Sludge Bed (ASB)-Reactors. Bacterial Metabolism in Wastewater Treatment Systems. and Drinking Water Management Unit II: Metal pollution control and soil decontamination (15L)  Metal Ion Removal–Removalby Biomass: Physico-Chemical Elimination Methods. Off-Gas. and Phosphate Removal . categories of pollutants  General Aspects –Historical Development of Wastewater Treatment Processes. Course Code Title Credits Environmental Biotechnology and Agricultural PSLSCEBTT403 4 Biotechnology (60L) Unit I: Water Pollution Control (15L)  Environmental Pollutants – sources generating pollutants. Biological and Chemical Phosphate Removal. Biofilms in Biofiltration. Different Countries and Contaminants. natural and man-made pollution. Special Aerobic Wastewater and Sludge Treatment Processes and Process Combinations. Laws. Statutory Orders and Directives on Waste and Wastewater Treatment  Processes of Wastewater Treatment Waste Water Sources and Composition . Monitoring of Contaminants.Biological Processes in Wetland Systems for Wastewater Treatment. Monitoring of Environmental Processes with Biosensors. Wastewater. Experience with Biofilters in Wastewater Treatment. Biofiltration Processes for Organic Matter Removal. CSTR-Reactors and Contact Processes in Industrial Wastewater Treatment. Hygienic Aspects of Drinking Water. 20 . Fixed Film Stationary Bed Reactors and Fluidized Bed reactors.Municipal Wastewater and Sewage Sludge.Microbial Fundamentals and Consequences for Application. Nitrogen.Cleaner Production Soil Decontamination – General aspects . Analytical Parameters for Monitoring of Wastewater Treatment Processes. Structure. and Dynamics of Microbial Communities in Wastewater Treatment. Trickling Filter Systems. Activated Sludge Processes. Nitrification and Denitrification . Autoaggregation of Microorganisms: Flocs andBiofilms Nucleic Acid-Based Techniques for Analyzing the Diversity. Agricultural Waste and Wastewater Sources and Management  Aerobic Carbon. Continuous Flow and Sequential Processes in Municipal Wastewater Treatment. Industrial Wastewater Sources and Treatment Strategies. Modeling of Aerobic Wastewater Treatment Processes  Drinking Water Preparation – Potable Water Treatment. Submerged Fixed-Bed Reactors. Anaerobic Processes:Anaerobic Metabolism and its Regulation.

Benefits – economic. Future Settlement Structures with Minimized Waste and Wastewater Generation Waste Gas Treatment – Process Engineering of Biological Waste Gas Purification. and Future Developments of Soil Bioremediation. Technology and Strategies of Composting. India and private sector. petroleum products. effect of metals and salt on the growth of microbes and higher organisms. anaerobic Fermentation of Wet or Semi-Dry Garbage Waste Fractions. Microbiological Aspects of Biological Waste Gas Purification. Characterization of the Geological and Hydrogeological Situation. In situ Remediation. Utilization of Treated Soil. Phytoremediation of Organics. Bioremediation. Limits. in situ and ex situ conservation – gene banks and management of germ plasm collection. Compounds with Nitro Functions  Processes for Soil Clean-Up – Thermal Processes. Genetically Engineered Microorganisms and Bioremediation. Sampling and Analysis of Soil  Biodeterioration– Microbial deterioration of timber. different adaptation mechanisms to tolerate higher concentrations of metals by organisms Unit : III Soil and gas waste treatment and bioconservation (15L)  Solid Waste Treatment Bio. Effects on Natural Geochemical Barriers and Remediation. Biofilters. Scrubbing/Extraction.current level of biodiversity. plastics and food products. Bioreactors. Anaerobic Degradation. Bioscrubbers. alpha and beta diversity. Combined Mechanical and Biological Treatment of Municipal Solid Waste. essential services provided by 21 .A Hierarchy of Technology Choices. Sanitary Landfilling of Solid Wastes . Possibilities. Ecotoxicological Assessment  Microbiological Aspects – Aerobic Degradation by Microoganisms. Bioavailability of Contaminants. Disposal.Long-Term Stability and Environmental Implications. Immobilization. Treatment of Waste Gas Pollutants in Trickling Filters.and Pyrotechnology of Solid Waste Treatment. Commercial Applications of Biological Waste Gas Purification  Biodiversity conservation. Degradation of Chlorinated Compounds.Long-Term Problems with Leachates. Bacterial Activity Enhancement and Soil Decontamination. Membrane Processes and Alternative Techniques. Microbiology of Composting. Advanced in situ Bioremediation . Sanitary Landfills . Landfill Systems. leather. Bioremediation with Heap Technique. Composting of Plant Residues and Waste Plant Materials. conservation mechanisms.Humification. conservation efforts by G-15 countries. degradation of Aromatic and Polyaromatic Compounds. Phytoremediation of Metals. Degradation of Aliphatics. Europe. extinction and endangered species –natural and human causes.

fixation of nitrogen. use of synthetic herbicides and its disadvantages. Ampelomycescomothyrum and others  Bio-insecticides– principles. fertilizer. electricity. advantages and disadvantages. evolution of symbiosis. stages in the investigation. surface culture. benefits. formulation and use bio-insecticides. animal manure. recycling of medical waste. Bioassays. advantages over chemical pesticides. biological control of vector communicable diseases such as malaria and Filiria – Mechanism of parasite action and difficulties associated with the successful use of biopesticides against these diseases. nitrogenase system. a reductive process. application to soil and seed  Biopesticides Chemical pesticides – uses. presently used candidates as biological control agents. biological and thermal processes used. applications – generation of heat. hydrogenase system. production of biological insecticides – submerged fermentation. safety testing of bio-insecticides. mode of action. leghaemoglobin. future  Bioherbicides – deleterious effects of weeds on crops and crop yield. methods of manufacture. P. natural ecosystems. molecular mechanisms of resistance development and strategy for integrated pest management. microorganisms involved. blue green algae as nitrogen fixers Biofertilizers – Symbiotic associations and its biotechnological relevance. humus and other products Unit : IV Agricultural Biotechnology (15L)  Nitrogen fixing bacteria – nitrogen cycle. alternative fuels. bioherbicides – microbes and insects as agents. genetic engineering to increase weed specificity 22 . biofertilizers–types. biological pesticides –types. molecular genetics and biochemistry. biocontrol of plant and grain fungal diseases – Trichoderma. nitrogen fixation. selective targeting. mechanical. fluorescence. in vivo culture. nitrogenase gene cluster. mechanism of action. ethical and aesthetic rewards  Biorecycling– definition. septic tank waste. mechanism of production. Biopesticides of plant origin – Neem. biodegradable waste. stability and formulation in natural organisms and genetically modified microorganisms. Nitrogen fixation – mechanism.

Microbial analysis of fish 4. organo phosphorus pesticide) 14) Neem pesticide against the fungus and bacteria (Bioassay method) against the grain pathogens. 11. chloride. organic matter. (Pesticide suitable for practical like Rogur. 13) Effect of spray of pesticide on the proline content of leaf.PSLSCEBTP403 Environmental Biotechnology and Agricultural 2 04 biotechnology (60L) 1. 23 . Analysis of canned fish: polyphosphates and sulphur dioxide 7. phosphorus. 5. Isolation of protease producers from soil 10. Determination of histamine in frozen fish.nitrogen. Ash analysis: (Dry ashing. halides. Determine of moisture content and sodium chloride in dried fish 6. & calcium carbonate content 2. Isolation of marine microorganisms. Soil analysis. BOD. COD. Separation and estimation of silica in grass or rice leaves or any other suitable leaf of crop plant. Wet ashing): a. b) Determination of potassium and phosphorous in organic matter c) Determination of sulphur in plant material 12) Determination of organic Carbon by Walkley and Black’s rapid titration method. Waste water analysis . Microbial analysis of sludge 3. determine potassium and phosphorous in plant material. 9. Hardness. alkalinity and chloride 8. Total solids. Ashing with magnesiuim nitrate.pH.

thin films and multilayers. Synthesis of nanoparticles (chemical. theory. biomimetics and drug delivery (15L)  Bionanotechnology–Introduction and Definition Types of bionanostructures synthesised (carbon nanotubes. Marine life forms (microbial. transdermal. organogels. Unit : III Other applications (15L)  Biosensors . nanoshells. colloidal systems. seed production technology of carps. Prawn culture . physical and biological strategies). Rate programmed delivery – pH. virus based systems). Pearl oyster culture . 24 . pearl producing species.definition. nanosomes and polymersomes.  Applications of Bionanotechnology in Diagnostics ( gold nanoparticles. liposomes. Isolation of Marine Natural Products (in medicine. cultivable fish species. quantum dots and buckyballs. Route: Across blood brain barrier. feedback regulated etc. dyes etc. exobiology. criteria for selection of species. history and scope. Pharmacy and medicine : Uses of nanoparticles in medicine (Silver nanoparticles. composition of pearl quality and prospectus. temperature. aquafarm engineering Pisiculture . Seaweed culture . cyclodextrins. biosensors [applications]) Applications of Bionanotechnology in Environment  Potential risks and future of Bionanotechnology. Tissue engineering of bone. cultivable prawn species. Seafood-borne illness adversely affects public health and coastal economies. Unit II: Bionanotechnology. economically important species culture and post harvest technology.  Applications of Bionanotechnology in Agriculture. adhesion (lizard foot pads) and water repulsion (lotus leaf). constraints and recent development. pearl culture technology. Electrochemical Methods . paramagnetic ironoxidecrystals dendrimers).). ionic strength. targeted drug delivery.)  Aqua culture technology. Course Code Title Credits PSLSCEBTT404 Special Processes and regulations and ethics (60L) 4 Unit I: Marine biotechnology (15L)  Extreme environmental conditions. The Biological Reaction.  Biomimetics: Concept. gold nanoparticles) Drug/ Gene delivery (Carriers: Dedrimers. Potentiometric Biosensors. mono and poly culture. lab on a chip. quantum dots.Introduction. spawning techniques. nanoshells.Amperometric Biosensors. culture methods in India. plant and animal). carps culture. nanowires. Nanoscale-structures in colour display (peacock/butterfly wings/ beetles).

Strategic Regulations for Safe Development of Transgenic Plants. Food Industry and Medicine: Organic foods. Current Trends in Manufactured Foods. identifying organic foods. Biotechnology and Bioethics: Product Development and Legal Requirements . legal. Regulations for Recombinant DNA Research.Structured Risk Assessment of rDNA Products and Consumer Acceptance of these Products. crops derived through biotechnology. Consumer Acceptance and Market Forces. Product Development and Production in the US. economics and ethical issues (15L)  Modern Biotechnology . Regulatory requirements  Biotechnology in a Developing World .  Genetic Modification in Agriculture. advantages of heaps and dumps. Immunotoxilogical assessment of therapeutic products. Introduction. Biotechnology in the Asian-Pacific Region.  Safety evaluation of Biotherapeutics. application. bioreactors in bioleaching. controversies over risks. biomining operation. Food industry . accumulation and transformations of metals by microbes. Allergenicity of recombinant products used in food and feed. food and food ingredients produced by GMOs. plants. gold. Biomedicinal Product Development. microbial corrosion and its inhibition. agribiotechnology products. Japan and Europe Biosafety assessment . types of organic foods. history. Unit : IV Biotechnology – Biosafety assessment. Conductimetric Biosensors. 25 . Optical Biosensors .Commercial Biotechnology: Developing World Prospects. background. Piezoelectric Biosensors.Evanescent Wave Biosensors and Surface Plasmon Resonance. Preclinical safety assessment of vaccines. Calorimetric Biosensors.Biosafety in rDNA Research and Production. microbes. Environmental impact and risk monitoring of recombinant products. Animal feeds. Food Animals. Immunosensors  Inorganic biotechnology. Whole Cell Biosensors. microbial leaching of metals (biomineralization). Foodcrops. Legal Requirements in the Production of Novel Foods and Processes. biotechnology of coal. organic food & preservatives. Biotechnology and Biological Diversity.

2006. Pearson Education. Form Research to Manuscript: A guide to scientific writing. Walker and R. P. Razdan 26 . Irfan Ali. 2008.A. An Introduction (2008): S. Concept Publishing Company 4. Vittal and R. 2.. Ignacimuthu.P.. Springer Science 10. Cengage Learning 9. 6. Research Methodology. Rastogi More Gene Manipulations in Fungi (1991): J. Misra R. Rapley Biotechnology for Beginners (2006): ReinhardRenneberg Biotechnology Vol 1: Rehm& Reed Biotechnology Vol 2: Rehm& Reed Biotechnology. Bhat Biotechnology.K. (2nd. Fundamentals of Biostatistics. Lasure Microbial Metabolism and biotechnology. 2005. PHI Learning Pvt. Das.. New Delhi. M. Wiley Eastern Limited. USA. Ltd. D.K. 2011. C. W. Excel Books.. Research Methodology. New Delhi. Allahabad..(2nd. 3. Singapore.. K. Kothari. R. Mukherjee and Company Pvt.New Delhi. Bennette and Linda L.). PSLSCEBTP 404 Dissertation of Research Project (60L) 2 04 Project studies: presentation and preparation of report of observations and results REFERENCES PSLSCEBTT301 1.. e-book: Horst Doelle Introduction to plant tissue culture: M. S. S. 7. R. Saravanavel.ed. Research Methodology. 1985. Bhattachraya. Rosner B. Ukaaz Publications 8.Methods and Techniques. 1990. Khan. C. Kumar..R. Principles and Applications (2007): S. Concepts and Applications (2009): R. Research methodology.).ed. Research Methodology: A Handbook. New Delhi. 5. Kitab Mahal PSLSCEBTT 302 Molecular Biology and Biotechnology. Ltd. 1989. an Introduction to Research. Panneerselvam R.M. Katz J. J. Research Methodology-A Step-by-Step Guide for for Beginners. Kolkata. Biotechnology. 2009. 1986. Fundamentals of Biostatistics. 2012. 4th edition (2002): J.

1989: Maniatis.Animal Cell Culture by Ian Freshney Basic Cell Culture.Davis 2nd. M. Principles and Applications (2007): S. Biotechnology. 4th edition. Oxford press Animal Cell Culture Sudha Gangal Molecular Biology: A laboratory Manual. Rapley Biotechnology for Beginners (2006): Reinhard Renneberg Biotechnology Vol 3: Rehm& Reed Biotechnology Vol 4: Rehm& Reed Biotechnology. Rastogi Microbial Metabolism and biotechnology. Ed. S. Walker and R. Vittal and R. e-book: Horst Doelle Industrial Microbiology: Cassida PSLSCEBTT 304 Molecular Biology and Biotechnology. Rapley Biotechnology for Beginners (2006): ReinhardRenneberg Biotechnology Vol6: Rehm& Reed Biotechnology Vol7: Rehm& Reed 27 . Green and J. R. 2ndedition. An Introduction (2008): S. Concepts and Applications (2009): R.Ed 2007. 4th edition (2002) and 5th Edition: J. Sambrook PSLSCEBTT303 Molecular Biology and Biotechnology. C.J.M. J. Rapley Molecular Biology and Biotechnology. M. Bhat Biotechnology. 2012: M. 4th edition (2002): J. Walker and R. Walker and R. M. Ignacimuthu. 5th edition: J. Fritsch and Sambrook Molecular Biology: A laboratory Manual.

2nd edition Vol 1 and 2 (2004): Dilip K. 2000.A. Orengo. 2003. Zvelebil and J.A.bioinfbook. Rastogi Handbook of Fungal Biotechnology. 2003 Chemoinformatics A Text Book. Pevzner. N. Baxevanis. 14. A. Cold Spring Harbor Laboratory Press. 28 . 2003. Technology in Transition. Concepts and Applications (2009): R. Gasteiger. D. R. Jones and J. eds. Bioinformatics: Sequence and genome analysis. Vittal and R. e-book: Horst Doelle PSLSCEBTT401 1. D. Proteins and Computers. 2002. J. An Introduction (2008): S. Introduction to Bioinformatics. S.F.M. and M. HP Rang. Xu. 2.org/) 3. Pavel Pevzner and Ron Shamir. Bioinformatics for Biologists. MIT press. A.Biotechnology Vol 8a: Rehm& Reed Biotechnology Vol 8b: Rehm& Reed Biotechnology Vol 9: Rehm& Reed Biotechnology.T. Elsevier Ltd 1st edition 2006 13.L. Introduction to Simulation. Raymer. 2004. Cambridge University Press. Oxford University Press. An Introduction to Bioinformatics Algorithms.E. Garland Science.D. Mount. Fundamental Concepts of Bioinformatics. C. M. Wiley 12. Pavel Pevzner and Ron Shamir. MIT press. Cambridge University Press. Bhat Biotechnology. Computational Molecular Biology: An Algorithmic Approach. Bioinformatics and Functional Genomics by Jonathan Pevsner (www. Drug Discovery and Development. Jones and P. 10. 6. Roultledge. MIT press. 2001. Principles and Applications (2007): S. 8. Understanding Bioinformatics. 2004 Bioinformatics: A Practical Guide to the Analysis of Genes and Proteins.Thornton. Baum. Jiang. Payer. Biotechnology. C. 2011 5. 2011. Second Edition. Bioinformatics: Genes. 2002. and Francis Ouellette. Pevzner. O. A. Lesk. Bioinformatics for Biologists. Arora Microbial Metabolism and biotechnology. Zhang. A. C. Pearson Education. T. B. Ignacimuthu. eds. McGraw Hill. eds. M. Current Topics in Computational Molecular Biology. 11. Y. D. 2008 4. P. 9.. Krane and M. 7. T.

H. Arora Microbial Metabolism and biotechnology. 4th edition (2002): J. 23. Barnes & Noble. Kubinyi. Biotechnology. Mannhold. Medical Biotechnology.Discrete Event System Simulation. David Nicol. Chemo Informatics in Drug Discovery (Methods and Principles in Medicinal Chemistry. and Kohli.. R. Randall Sadowski. .V Methods in Biotechnology and Bioengineering. 22.Simulation Modeling and Analysis.. Rastogi Handbook of Fungal Biotechnology. A. Oxford University Press. Medical Biotechnology. and Buthe. T. Walker and R. McGRAWHILL Geffery Gordon. Venugopal Rao. C. al. [3rdEdition] 24. 2001 Bioinformatics: From Genomes to Drugs.15. W David Kelton. Ignacimuthu.P. An Introduction (2008): S. John Wiley & Sons 21.. 19. A. Kohane.M..System Analysis and Modeling. (2008) Judit Pongracz.Simulation with Arena. Jerry Banks. D. Stan Tsai. S. Bhat Biotechnology. . and Folkers. Trafford Publishing. Hassan.J.O. 16. PHI 26. M. 18.System Simulation with Digital Computer. Tudor. J. Averill Law. 20. . W. Lengauer. PHI 25. F. J. 2004 Bioinformatics: Principles and Basic Internet.. Vittal and R. Principles and Applications (2007): S. 2004 Practical Bioinformatics (Series: Nucleic Acids & Molecular Biology Vol. N. Rapley Biotechnology for Beginners (2006): ReinhardRenneberg Biotechnology Vol5a: Rehm& Reed Biotechnology. I. Deborah Sadowski. Himalaya Publishing House. Churchill Livingstone. C. 15) Springer. Introduction to Computational Chemistry. et.. Concepts and Applications (2009): R.System Simulation. . R. Elsevier (2009) Pratibha Nallari & V. Bernard Zeigler. John Carson. I. 17.. Academic Press Harcourt India 27. Bujnicki. G. Body. India (2010) 29 . John Wiley & Sons. 2002 Microarrays for an Integrative Genomics. Vyas. Inc. Mumbai... Herbert Praehofer. Kho. McGRAWHILL PSLSCEBTT 402 Molecular Biology and Biotechnology. MIT Press. S. . Medical Biotechnology. . Mary Keen. Barry Nelson.S.Theory of Modeling and Simulation. An Introduction to Computational Biochemistry 1st Edition. David Kelton. Donald W. e-book: Horst Doelle Jogdand S. Tag Gon Kim. Jensen. 2nd edition Vol 1 and 2 (2004): Dilip K. . Academic Press Narsing Deo.. A.S. Wiley-VCH.

S. M. Walker and R. Vittal and R. Concepts and Applications (2009): R. 4th edition (2002): J. C. Rastogi Handbook of Fungal Biotechnology. Walker and R. Principles and Applications (2007): S. Rao. Bhat Biotechnology. C. Rapley Biotechnology for Beginners (2006): ReinhardRenneberg Biotechnology Vol11a: Rehm& Reed Biotechnology Vol 11b: Rehm& Reed Biotechnology Vol 11c: Rehm& Reed Biotechnology. M. Biotechnology. An Introduction (2008): S. Rapley Biotechnology for Beginners (2006): ReinhardRenneberg Biotechnology Vol10: Rehm& Reed Biotechnology Vol 12: Rehm& Reed 30 . J. R. e-book: Horst Doelle PSLSCEBTT404 Molecular Biology and Biotechnology. New Age International Publishers. 4th edition (2002): J. 2nd edition Vol 1 and 2 (2004): Dilip K. Microbial Metabolism and biotechnology. Ignacimuthu. Arora Environmental Sciences: Odum Environmental Biotechnology: Alan Scragg Environmental Biotechnology: Bimal Bhattachraya and Ritu Banerjee Environmental pollution control engineering.PSLSCEBTT 403 Molecular Biology and Biotechnology. S.

Rastogi Bionanotechnology: Lessons from Nature (2004): David Goodsell Bionanotechnology. (2002): J. Fuchs 31 . Principles and Applications (2007): S. C. L. Lewis Biotechnology and safety assessment. Papazoglou Encylopedia of Nanotechnology. Proteins to Nanodevices (2006): V. 4 volume set (2012): B.Biotechnology. Bhusan BioNanotechnology. Renugopalakrishnan and R. 3rd edition. Thomas and Roy. Volume 7 of Synthesis Lectures on Biomedical Engineering Series: Elisabeth S. A.

G. Overall conduct as a responsible learner. learners/ publication of a research paper/presentation of a research paper in seminar or conference 30 (a) Selection of the topic. Introduction. 50 50 .60 % [Semester End Theory Assessment]: 60 marks 32 . 50 50 . 50 50 . write up. 50 50 . OVERALL EXAMINATION AND MARKS DISTRIBUTION PATTERN Semester III PSLSCEBTT301 PSLSCEBTT302 PSLSCEBTT303 PSLSCEBTT304 External External External External Internal Internal Internal Internal Course Grand Total Total Total Total Total Theory 40 60 100 40 60 100 40 60 100 40 60 100 400 PSLSCEBTP301 PSLSCEBTP302 PSLSCEBTP303 PSLSCEBTP304 Practicals . 50 50 . 50 50 200 Semester IV PSLSCEBTT401 PSLSCEBTT402 PSLSCEBTT403 PSLSCEBTT404 External External External External Internal Internal Internal Internal Course Grand Total Total Total Total Total Theory 40 60 100 40 60 100 40 60 100 40 60 100 400 PSLSCEBTP401 PSLSCEBTP402 PSLSCEBTP403 PSLSCEBTP404 Practicals . Particulars Marks 1. Active participation in routine Class instructional deliveries 05 2. references (15) (b) Presentation with the use of ICT (15) B] External Examination . 05 3. One seminar based on curriculum to be assessed by the teacher of the institution teaching P. 50 50 200 MODALITY OF ASSESSMENT: THEORY EXAMINATION PATTERN: A] Internal Examination for Theory: 40 marks No. Communication & leadership qualities in organizing related academic activities. 50 50 .

c. c & d only. each carrying six marks OR a. Laboratory work 40 2. The students are required to present a duly certified journal for appearing at the practical examination. Each question will be of 24 marks with options. SEMESTER IV: Practical examination will be held at the college / institution at the end of the semester. PRACTICAL EXAMINATION PATTERN A] Internal Examination: There will not be any internal examination/ evaluation for Practicals. Journal 05 3. Viva 05 SEMESTER III: Practical examination will be held at the college / institution at the end of the Semester. (b) All questions shall be compulsory with internal choice within the questions. e & f only each carrying four marks and the allocation of marks depends on the weightage of the topic. (c) Questions may be sub divided into sub questions a. d. failing which the student will not be allowed to appear for the practical examination. Theory question paper pattern:- (a) There shall be five questions each of 12 marks. b. In case of loss of Journal and/ or Report. Duration . Research proposal (Dissertation based on Literature Review): Candidates are required to present duly certified research proposal (as per the BCUD format) with relevant references (minimum 25) and make the power point presentation of the same for the evaluation by the examiner (the research proposal must be included with literature survey of the selected research topic). 2. B] External (Semester end practical examination) per course: No.These examinations shall be of two and half hours duration. The students are required to present a duly certified journal for appearing at the practical examination.1. b. a Lost Certificate should be obtained from Head of the Department/ Co-ordinator of the department. failing which they will not be allowed to appear for the examination. On each unit there will be one question & fifth one will be based on all the four units . 33 . failing which they will not be allowed to appear for the examination. Particulars Marks 1.

Research Project work (Dissertation based on Research): Candidates are required t present duly certified dissertation report based on the topic of research along with the laboratory notebook containing raw data and make the poster presentation of the research work for evaluation by the examiner. 34 . failing which the student will not be allowed to appear for the practical examination. a Lost Certificate should be obtained from Head of the Department/ Co-ordinator of the Department.In case of loss of Journal and/ or Report.