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DCS’s A.R.A.

College of Pharmacy, Nagaon, Dhule


STANDARD OPERATING PROCEDURE
TITLE: UV VISIBLE SOP NO. : SOP/02A/01
SPECTROPHOTOMETER
MAKE/ MODEL: SHIMADZU 1800 REG NO. : ARACOP/UVSP/158/07-08

Scope: To determine the absorbance of solution


Procedure:
1. First turn “ON” all the switches along with main switch.
2. Adjust the wavelength from 200-800 nm(UV or Visible)
3. Switch “ON” the power switch “ON” switch and for UV range
“ON” preheat for 30 min. before taking the absorbance.
4. Previously select lamp for UV-D2 and for visible W.
5. Wash the cuvette with water and then acetone which select for
desired wavelength either for UV cuvette or visible cuvette.
6. Cuvette should be handle with care and place in proper manner
7. Prepare the required strength solution.
8. Set zero with blank and ensure.
9. Take the reading of standard and ensure for two times, the
difference between two readings should not be vary.
10.Then take the reading of sample solution which is unknown.
11.After taken the reading of sample solution wash the cuvette with
water and then acetone.
12.Plot the graph concentration v/s absorbance.
13.Lastly turn “OFF” the “ON” switched, powers witch and then
main switch.
No change permitted.

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DCS’s A.R.A. College of Pharmacy, Nagaon, Dhule
STANDARD OPERATING PROCEDURE
TITLE: DISSOLUTION TEST SOP NO. : SOP/01/01
APPARATUS
MAKE/ MODEL: ELECROLAB REG NO. : ARACOP/DST/201/09-10

Objective: To obey the standard operating procedure for dissolution


test apparatus

Scope: To operate dissolution test apparatus

Procedure:
1. Ensure that the instrument is clean and free from dust.
2. Switch ON mains and heater switch provided on the rear right
hand side. When the power is switch on the followings-
3. THE STEP PROGRAM display show SD and remaining
displays and blank of indicating that the system is going through
“self-diagnosis”.
4. The system gives a long beep.
5. Lift the stirrer unit using the stirrer unit using the “UP” key on
the front panel.
6. Start the test using the START/ STOP/ TEMP. Key.
7. Carry out the analytical procedure as per the specification and
calculate the result. “SWITCH OFF” the instrument when not
in use.

No change permitted.

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DCS’s A.R.A. College of Pharmacy, Nagaon, Dhule
STANDARD OPERATING PROCEDURE

TITLE: FTIR SOP NO. : SOP/02/01/A/04


MAKE/ MODEL: BRUKER OPTIC REG NO. : ARACOP/FTIR/260/2012
GMBH GERMANY, ALPHA-T

Objective: To take IR spectrum for structural elucidation.


Scope: To approve for the use in laboratory.

Procedure:
1. Sign on the note book.
2. Turn on the computer and sign in.
3. Turn on the power of instrument, wait until initialization
succeeded. In small panel of instrument “Perkin Elmer
Spectrum 100 Series” will be shown if the initialization
succeeded, otherwise come to see Jianhua first.
4. Clean the sample holder by acetone with Kim wipes, make sure
not to splash the acetone on the instrument.
5. Launch the “Spectrum” software on the desktop with user name
of “Analyst “and password “Analyst” then select “Spectrum
100”.
6. Select “Instrument setup” button, “Scan and instrument setup”
dialog will pop up, input your sample name, scan range, (650-
4500 cm-1) and scan number.
7. Click the “back ground” button to collect back group
information of sample holder.
8. Place your sample on sample holder. If sample is liquid you can
go ahead and press “Apply” then start.to collect the spectrum. If
your sample is solid click the “ monitor” button on the “Scan
and instrument setup” dialog first, and then lower down the
pressure arm , through the “monitor” dialog to monitor the total
pressure applied to the sample, set the “Force Gauge” to be
PREPARED BY CHECKED BY APPROVED BY
DCS’s A.R.A. College of Pharmacy, Nagaon, Dhule
STANDARD OPERATING PROCEDURE
around 80, then click “Finish”. Press “Apply” and then “Start”
to collect spectrum.
9. Data Processing:
10.Peak Lable: select “ View” from the menu , then label “label
peaks”. If you want to label special peak “
view”----“cursor”---“vertical continuous”, then move the cursor
to peak you want and then “view” ----“ label cursor”.
11.Data saving: you can save your data as ASCII by
“FILE”---“Save as” the select “ASCII (*.ASC) as the save type.
12.Turn off the software, log off the computer and switch off the
instrument. Make sure to clean the sample holder completely
befor you leave.

No change permitted.

PREPARED BY CHECKED BY APPROVED BY


DCS’s A.R.A. College of Pharmacy, Nagaon, Dhule
STANDARD OPERATING PROCEDURE

TITLE: SINGLE PAN DIGITAL SOP NO. : SOP/02/01/A/04


BALANCE
MAKE/ MODEL: Tapson TSP 700 REG NO. : ARACOP/SPB/48B/97-98
Classic

Objective: For weighing of all samples that is raw material and


finished product as per pharmacopoeia.

Scope: Weighing.

Procedure:
1. Before taking the weight ensure that whether the balance is
leveled.
2. Ensure that whether zero coincides with either zero.
3. The reading scale should swing properly.
4. Ensure that there should clean weight pan . keep it clean with
the help of brush, the surrounding should clean while taking the
weight.
5. Put “ON” the switch of mains while taking weight then adjust
the weight as required. While taking the weights closed both
sides of balance.
6. After taking the weight the balance should be clean with brush
and befor leaving the balance put “OFF” the main switch.
7. While operating the balance , operate it smoothly, and rotate the
knob slowly.

No change permitted.

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