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    Hamza Harb
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    lab test ELISA described

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    lab test ELISA described

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    11 views11 pages

    ELISA Lab Compatibility Mode

    Uploaded by

    Hamza Harb
    lab test ELISA described

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Flag for inappropriate content
    of 11

    ELISA

    1. Can be used to test for all pathogens and some


    toxins (mycotoxins).
    2. Three types:
    a) Antibody capture
    b) Antigen capture
    c) Competitive ELISA for Ab detection

    1
    2
    3
    Decay Of Maternal Ab for IBV

    0 doa 5 doa

    10 doa 15 doa

    Decay Of Maternal Ab for IBV

    7000

    6000

    5000

    4000
    GMT
    3000

    2000

    1000

    0
    0 doa 5 doa 10 doa 15 doa

    4
    Decay Of Maternal Ab for AIV

    0 doa 5 doa

    10 doa 15 doa

    Decay Of Maternal Ab for AIV

    700

    600

    500

    400
    GMT
    300

    200

    100

    0
    0 doa 5 doa 10 doa 15 doa

    5
    Decay Of Maternal Ab for IBD

    0 doa 5 doa

    10 doa 15 doa

    Decay Of Maternal Ab for IBD

    20 doa 25 doa

    25000

    20000

    15000

    10000 GMT

    5000

    0
    30 doa 0 5 10 15 20 25 30 35
    doa doa doa doa doa doa doa doa

    6
    Transfer of Maternal Ab for IBV

    25000

    20000

    15000
    Breeders
    Chicks
    10000

    5000

    Transfer of Maternal Ab for AIV

    2500

    2000

    1500
    Breeders
    Chicks
    1000

    500

    7
    Transfer of Maternal Ab for IBD

    40000

    35000

    30000

    25000
    Breeders
    20000
    Chicks
    15000

    10000

    5000

    Transfer of Maternal Ab for IBV

    25000

    20000

    15000
    Breeders
    Chicks
    10000

    5000

    8
    10
    9
    8
    7
    6
    Titer 5 ND
    4 AI
    3
    2 IB
    1
    0
    0 5 10 15 20 25 30 35 40
    ND ND ND ND Age (days)
    IB IB IB IB
    Live Live AI Live
    killed

    21 doa 28 doa 34 doa


    24.0 12.0 12.0

    10 10
    20.0 10.0 10.0
    9 9
    16
    Number of Samples
    Number of Samples
    Number of Samples

    16.0 8.0 8.0


    13
    6
    12.0 6.0 6.0

    4
    NDV 8.0 4.0
    3 3
    4.0

    4.0 2.0 2.0


    1 1 1 1 1
    1
    0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
    0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14
    Titer Groups Titer Groups Titer Groups

    30.0 24.0 24.0

    26 21
    25.0 20.0 20.0
    Number of Samples

    Number of Samples

    Number of Samples

    20.0 16.0 15 16.0

    15.0 12.0 12.0

    IBV 10.0 8.0


    6 6
    8.0

    4
    5.0 4 4.0 4.0
    1 1 1 1 1 1 1
    0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
    0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14
    Titer Groups Titer Groups Titer Groups

    9
    21 doa 28 doa 34 doa
    12.0
    24.0 12.0

    10.0
    20.0 19 10.0

    8 8

    Number of Samples
    8.0
    Number of Samples

    Number of Samples
    16.0 8.0
    7 7
    6 6.0
    12.0 11 6.0 5
    4
    4.0
    NDV 8.0 4.0

    2
    3
    2 2.0
    3

    4.0 2.0 1
    1 1
    0 0 0 0 0 0 0 0 0
    0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
    0 1 2 3 4 5 6 7 8 9 10 11 12 13 14
    0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14
    Titer Groups
    Titer Groups Titer Groups

    Samples: 29 Samples: 29
    Samples: 30
    Mean: 5004 Mean: 5182
    Mean: 285
    GMT: 2187 GMT: 3785
    GMT: 11

    StDev: 4007 StDev: 3372


    StDev: 478
    %CV: 58.33 %CV: 49.11
    %CV: 110.67

    21 doa 28 doa 34 doa

    24.0 23 24.0 24.0

    20.0 20.0 19 20.0


    Number of Samples
    Number of Samples

    Number of Samples

    16.0 16.0 16.0


    14

    IBV 12.0 12.0 12.0

    8.0 8.0 8.0

    4 4 4 4
    4.0 3 4.0 4.0 3
    2 2 2
    1 1 1 1 1 1
    0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
    0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14
    Titer Groups Titer Groups Titer Groups

    Samples: 30
    Samples: 30 Samples: 30
    Mean: 187
    Mean: 1295 Mean: 3521
    GMT: 4
    GMT: 364 GMT: 1005

    StDev: 537
    StDev: 1851 StDev: 4761
    %CV: 152.86
    %CV: 81.32 %CV: 89.12

    10
    21 doa 28 doa 34 doa
    30 24.0
    30.0 30.0 29

    25.0 25.0 20.0

    Number of Samples
    Number of Samples

    Number of Samples
    20.0 20.0 16.0
    14
    12
    15.0 15.0 12.0

    IBD 10.0 10.0 8.0

    4
    5.0 5.0 4.0

    1 0 0 0 0 0 0 0 0 0 0 0 0
    0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
    0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 0 1 2 3 4 5 6 7 8 9 10 11 12 13 14
    Titer Groups Titer Groups Titer Groups

    Samples: 30 Samples: 30
    Samples: 30
    Mean: 0 Mean: 1407
    Mean: 3467
    GMT: 0 GMT: 1040
    GMT: 3251

    StDev: 0 StDev: 683


    StDev: 1215
    %CV: 277.09 %CV: 39.65
    %CV: 30.11

    21 doa 28 doa 34 doa


    30.0 18.0 30.0 29
    28
    17
    25.0 17.0 25.0
    Number of Samples

    Number of Samples

    Number of Samples

    20.0 16.0 20.0

    15.0 15.0 15.0

    10.0 14.0 10.0


    AIV 5.0 13.0 5.0
    2
    12 0
    1 2 1 2 1 2
    Titer Groups Titer Groups Titer Groups

    Samples: 29 Samples: 29
    Samples: 30
    Mean: 1399 Mean: 3229
    Mean: 27
    GMT: 84 GMT: 2796
    GMT: 1
    StDev: 1586 StDev: 1545
    StDev: 104
    CV (%): 88.02 CV (%): 34.93
    CV (%): 81.15

    11

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