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BIOCHEMICAL ENGINEERING

Physical and Chemical Principles


Quice Review
Room 608-610, Doña Amparo Building,
G. Tolentino Street, Sampaloc Manila

14. C. Uronic acid


15. A. (+)-Mannonic acid
16. A. Kiliani-Fischer synthesis
17. C. Ruff degradation
18. C. Both A and B
19. D. (+)-Sucrose
20. B. D-(+)-glucose and D-(–)-fructose
21. C. Starch
22. B. Glycogen
23. C. Proteins
24. B. Glu-Cys-Gly
25. D. Sickle-cell anemia
26. D. Nucleic acids
27. B. Nucleotide
28. A. 65.0 mM/s
29. B. 0.100 mM
30. A. 46.43 mM

Solution to Linked Problem 28-30:

28.

Eadie-Hofstee plot:
𝑉 𝑉 𝑉max
=− +
[S] 𝐾𝑀 𝐾𝑀

[S] (mM) V (mM/s) V/[S] (s–1)


0.02 10.83 541.5
0.04 18.57 464.25
0.07 26.76 382.2857
0.10 32.50 325
0.15 39.00 260
0.20 43.33 216.65
0.30 48.75 162.5
0.50 54.17 108.34
0.70 56.88 81.2571

Linear regression data (V/[S] vs. V):

Slope = –1/KM = –9.996 mM–1


y-Intercept = Vmax/KM = 649.806 s–1

1
𝑉𝑚𝑎𝑥 = (649.806 s −1 )𝐾𝑀 = (649.806 s −1 ) ( ) = 65.0 mM/s
9.996 mM −1

29.

Hanes-Woolf plot:
[S] [S] 𝐾𝑀
= +
𝑉 𝑉max 𝑉max

[S] (mM) V (mM/s) [S]/V (s)


0.02 10.83 1.8467×10–3
0.04 18.57 2.1540×10–3
0.07 26.76 2.6158×10–3
0.10 32.50 3.0769×10–3
0.15 39.00 3.8462×10–3
0.20 43.33 4.6157×10–3
0.30 48.75 6.1538×10–3
0.50 54.17 9.2302×10–3
0.70 56.88 1.2307×10–2

Linear regression data ([S]/V vs. [S]):

Slope = 1/Vmax = 0.0154 s/mM


y-Intercept = KM /Vmax = 1.5389×10–3 s

1
𝐾𝑀 = (1.5389 × 10−3 s)𝑉max = (1.5389 × 10−3 s) ( ) = 0.100 mM
0.0154 s/mM

30.

Lineweaver-Burke plot:
1 𝐾𝑀 1 1
= +
𝑉 𝑉max [S] 𝑉max

[S] (mM) V (mM/s) 1/[S] (mM–1) 1/V (s/mM)


0.02 10.83 50 0.0923
0.04 18.57 25 0.0539
0.07 26.76 14.2857 0.0374
0.10 32.50 10 0.0308
0.15 39.00 6.6667 0.0256
0.20 43.33 5 0.0231
0.30 48.75 3.3333 0.0205
0.50 54.17 2 0.0185
0.70 56.88 1.4286 0.0176

Linear regression data (1/V vs. 1/[S]):

Slope = KM /Vmax = 1.5384×10–3 s


y-Intercept = 1/ Vmax = 0.0154 s/mM

1 1 1
= (1.5384 × 10−3 s) + 0.0154 s/mM = (1.5384 × 10−3 s) ( ) + 0.0154 s/mM = 0.02155 s/mM
𝑉 [S] 0.25 mM

1
𝑉= = 46.4 mM/s
0.02155 s/mM