Laboratory Operations & Safety Protocols

Chemistry Department Loyola School of Science & Engineering Ateneo de Manila University Version 2010

Preface to the 2010 Edition
In 2004, the government started to implement various laws, rules and regulations covering a broad range of activities related to the purchase, use, storage, waste management, and disposal of chemicals. The increased regulation has led the University to implement a common policy and procedure to address these various concerns. The Chemical Management and Inventory System (CMIS) was developed to address the need for accurate inventory, efficient purchasing, management, and compliance with various laws as well as the management of hazardous chemical wastes. The changes in the legal and regulatory climate have also contributed to the changing culture of safety which now puts emphasis on experiment planning, risk assessment, hazard consideration and chemical waste minimization. In this edition, information on different sections (i.e. Emergency Protocols, Use of Instruments and Waste Disposal) was updated. A section on the CMIS and Handling of Biological Samples was added. I wish to thank Ms. Mailyn Terrado for the section on Dealing with Biological Samples and Wastes. I also wish to thank Dr. Regina C. So and Dr. Fabian M. Dayrit for taking the time to proofread the manual. Jaclyn Elizabeth Santos 18 June 2010

Preface to the 2002 Edition


his manual is intended to be a safety reference document for lab workers at the Chemistry Department of the Ateneo de Manila University. It is mainly adapted from the Laboratory Safety Manual at the University of North Carolina at Chapel Hill, and the memo on Operating Practices issued by Dr. Ma. Assunta Cuyegkeng when she was Chair of the Department in 1996—practices that have served the Department well since the time of Fr. Schmitt. This manual provides basic information about hazards encountered in the lab and safety precautions to prevent accidents. It also contains the description of faculty, student, and staff roles in ensuring a safe working environment in the lab. It is not possible to cover all safety and emergency scenarios, but this manual should, nonetheless, serve as the starting point for the faculty in developing their own safety plans specific to the materials and procedures in their laboratories. It is often a sticky effort to balance convenience and control with regards to safety issues in the lab, but when all is said and done, the basic rules are alertness and vigilance, common sense, conservation of resources, and concern for other workers in the lab.

Francis Ted Limpoco
18 November 2002

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1. Introduction
1.1 1.2 1.3 1.4 1.5 Safety Awareness The Role of Faculty The Role of a Safety Officer The Role of Lab Technicians The Role of Students/Researchers

6 6 7 8 9

2. Emergency Protocols
2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 Dealing with Emergency Emergency Telephone Numbers Fire Emergency Chemical Spill Escape of Noxious Gases First Aid Medical Attention After First Aid Reporting Accidents and Injuries

10 11 12 13 14 14 15 16

3. The Use of Building & Facilities
3.1 3.2 3.3 3.4 3.5 3.6 Schmitt Hall Room Addresses Use of the Building Access Keys Special Rooms Use of Instruments

17 18 19 19 20 20

4. General Safety Principles
4.1 4.2 4.3 4.4 4.5 4.6 4.7 4.8 4.9 Overnight Operations Working Alone Eating, Drinking, and Smoking Housekeeping Warning Signs and Labels Shielding for Safety Compressed Gases Systems Under Pressure Cold Traps and Cryogenic Hazards

23 23 23 23 23 24 24 24 25 25

4.10 Glassware

5. Chemical Management and Inventory System
5.1 5.2 Chemical Management and Inventory System Controlled Chemicals

26 28

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Gas.7 Clean-up Procedure 8.3 Handling Specific Wastes 7.5 Laboratory Equipment 8. Waste Disposal 7.1 General Information 8.4 Labeling 7.2 A.6.6 Waste Disposal 8.1 Waste Characteristics 7.6 Disposal to Sewerage System 7.5 Waste Containers 7.2 Routes of Exposure 6.4 Handling Toxic Materials 6.6 Handling Flammable Materials 6.7 Disposal of Chemical Wastes 40 40 40 40 41 41 41 42 8.2 Storage 8.4 Handling 8.1 A.3 Form for Overtime/Overnight Lab Work Form for Reporting Lab Accidents Instrument Authorization Form 48 48 48 48 Figures Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 Figure 7 Figure 8 Figure 9 Figure 10 Figure 11 Lab Benches Fume Hoods Power. Handling of Chemicals 6.1 Hazardous Chemicals 6.5 Handling Carcinogens 6.3 Protective Clothing and Equipment 6.7 Handling Explosive/Reactive Materials 30 30 32 33 35 36 37 38 7. Biological Samples: Handling and Waste Disposal 44 8. and Water Lines Fire Extinguishers & Showers Floor Plans with Fire Exits Aspirator Set-up for the Clean-up of Mercury Spills Fire Alarm Compressed Gases CMIS’ Logical Computer-Hardware Set-up Overview of Requesting Procedure Overview of CMIS Requesting Procedures 52 52 52 52 53 53 53 54 54 55 55 56 Laboratory Operations & Safety Protocol 4 of 65 V1:2010 .3Transport 8.8 Body Fluids 44 44 44 44 45 46 46 47 Appendices A.2 Waste Reduction 7.

rules and regulations which govern various aspects related to chemicals 57 Table 3 Table 4 Table 5 Table 6 Implementing Rules and Regulation for specific substances List of PDEA-controlled chemicals DENR Classification of Hazardous Wastes 58 58 59 64 Summary of Recommended Biosafety Levels for Infectious Agents References 65 Laboratory Operations & Safety Protocol 5 of 65 V1:2010 .Tables Table 1 CMIS User-levels 57 57 Table 2 List of laws.

” when it is obvious what you should do in a given situation. Not entering the stockroom. Common sense is still the guiding norm. Maintain discipline and enforce the Department’s operational and safety rules. and check-out the same at the end of the term. and the locations of the valves for the gas. NO SET OF RULES can cover all situations. Every instructor should: 1. b.1 Safety Awareness he most important rule is that everyone involved in lab operations—from the highest administrative level to the individual workers—must be safety minded. This attitude can lead to a false sense of security. Please do not argue. Set a good example by being enthusiastic about safety. As lab instructor. (See Figures 1-5. closed-toe shoes and apron). shower. such as: a.) 4. Inspect the equipment in the students’ and common lockers.g. Orient students in the proper use of the lab facilities and instruments. This includes showing the locations of the fire extinguisher. Safety awareness can become part of everyone’s habits only if the issue of safety is discussed repeatedly and only if senior and responsible staff demonstrate a sincere and continuing interest in safety. Check-in students at the start of the term. d. which frequently results in carelessness. Every lab worker has a basic responsibility to himself and his colleagues to plan and execute lab operations in a safe manner. a. Brief students on the lab operations and safety protocols. eyewash. safety glasses. water. As the saying goes: “An ounce of prevention is worth more than a pound of cure”. “We were not told. and evacuation plan.2 The Role of Faculty The faculty is responsible for the administration of the safety program of the Chemistry Department. 1. Remind students to settle the lab breakage deposit immediately. the faculty should be in the laboratory for the entire laboratory period. Over familiarity with a particular laboratory operation may result in overlooking or underrating its hazards. but also the other people involved— always consider their safety and convenience as well. c. Wearing the prescribed safety gear: safety glasses and aprons. Most of the rules/responsibilities stated here are nothing more than common sense and concern for others. 3. Ask if students have special medical conditions that may interfere with lab work/safety. b. and power lines. and by observing all safety rules and wearing personal protective equipment (e. Not eating or drinking during the lab. Laboratory Operations & Safety Protocol 6 of 65 V1:2010 . c. 2.Chapter 1 Introduction T 1. Thus. be alert to unsafe conditions and actions and call attention to them so that corrections can be made as soon as possible. You must consider not just yourself.

10. and ensure there are logistical provisions for their safety in such cases. waste minimization and where appropriate. where recommended). 3. especially those doing their thesis) research assistants and faculty members regarding chemical safety and chemical waste management and minimization. especially those that may pose safety hazards. Discuss with researchers any safety risks involved in the experimental procedures that will be carried out and the materials that will be used in the research. As mentor/research supervisor. no eating and drinking in the lab/stockroom) and wearing protective equipment (e. and Laboratory Operations & Safety Protocol 7 of 65 V1:2010 . Orient the students on the chemical waste disposal procedures of the department. 1.g. The mentor is responsible for checking-in researchers on the instrument or ensuring that they are checked-in by a competent/expert user of the instrument.e. Notify the parents/guardian of the injured person about the incident. 7. Accidents resulting in medical treatment or observation should be recorded. Regularly orient science majors (both undergraduates and graduate students. effect. aprons. storage and disposal of the Chemistry department. Undertake an annual review of the laboratory experiments which are included in curricular offerings to consider aspects of safety. 6. and recommendations for the prevention of reoccurrence. 9. chemical management. stating the cause. inspect often and intelligently. Conduct a briefing on safety issues at the start of the class. 8. 4. Set a good example by being enthusiastic about safety. 6. spill kits) 4. first aid kits. check that the system is working satisfactorily.g. hoods. Orient researchers with the general operations and safety procedures at the Chemistry Department.5. eye wash stations. fire alarms. 2. eyewear. but also ensures that the student is not exposed to safety risks in doing his research. compliance recommend appropriate improvements. Train students in proper lab techniques. Coordinate with the University Physical Plant and Office of Adminitrative Service regarding the disposal of chemical wastes with a DENR-accredited waste transporter and treater. A formal written report to the Chair should be made of all accidents. Conduct an inventory of chemical wastes generated by the department. 2. Orient researchers with the instrument techniques and protocols. Anticipate and prevent accidents at all times. Report all accidents. Manage the CMIS. 7. The safety officer should: 1. the faculty not only guides the student in the direction of his research project.3 The Role of a Safety Officer The safety officer is responsible for activities related to safety. The mentor/supervisor should: 1. regularly check safety facilities and equipment (i. redesign or replacement. and by observing all safety rules (e. and take effective corrective action promptly. etc. 3. be alert and vigilant for unsafe conditions. implement proper first aid measures and if necessary coordinate proper and speedy conveyance of the injured person to a medical facility. 8. fire extinguishers. Together with the laboratory supervisers and staff. Determine whether researchers need to work continuously after office hours or overnight. Carefully review all laboratory experiments for possible safety problems before experiments are assigned. safety showers. In case of accidents/emergencies. 5. Review and update safety protocol and procedures in the department.

Dispense chemicals. Accidents resulting in medical treatment or observation should be recorded. 5. supplies. and sand for cleaning up spills. such as: fire extinguishers. 4. mechanical repairs. d. Provide technical services at Schmitt Hall. c. where recommended). This includes fire extinguishers. In case of accidents/emergencies. first aid kits. especially for laboratories. and repairs on metal.9. and equipment. 8. According to established norms. a. 10. and others. and deliveries. 6. fume hood. and plastic. Laboratory Operations & Safety Protocol 8 of 65 V1:2010 . and recommendations for the prevention of reoccurrence. special containers for chemical and broken glass wastes.g. e. 7. Set a good example by being enthusiastic about safety. Inform the immediate supervisor well in advance of the need to order supplies before they run out. do various operations such as purification of solvents by distillation. Ensure that teaching laboratories are provided with properly-labeled chemical waste bottles. investigate and repair simple electrical circuits and minor electrical breakdown. glassware repairs. 3. Together with the safety officer. f. assist the faculty/lab supervisor in implementing proper first aid measures and if necessary assist in the proper and speedy conveyance of the injured person to a medical facility. The lab technician should: 1. and by observing all safety rules (e. b. emergency showers.4 The Role of Lab Technicians The lab technician and/or stockroom manager should also be present during the entire laboratory period to assist the faculty in managing the experiment and administering the safety procedures of the Department. Exercise supervision over students with regard to the stockroom transactions and general order in the laboratories.. 2. Remind students to settle their lab breakage deposits immediately. Under the supervision of faculty member. materials. stating the cause. 1.g. no eating and drinking in the lab/stockroom) and wearing protective equipment (e. inspect all safety implements according to the recommended frequency. a. Prepare with accuracy chemicals and solutions for laboratory work. c. eyewear. etc. b. Handle the renewal of licenses required by government authorities. Report all accidents. Assist the faculty in inspecting the student laboratory lockers/equipment at the beginning and at the end of the semester. aprons. Ensure that safety materials and paraphernalia are well in stock and replenished when used up. and that these are placed in easily accessible locations in the laboratory. Ensure that the CMIS is updated. effect. A formal written report to the Chair should be made of all accidents. eye wash. fine students for failure to observe laboratory rules or inform the lab instructor of any violations to enforce penalty. Make plumbing repairs. Manage the stock of chemicals and materials: keep records of materials. Prepare chemical unknowns which must be carefully coded and recorded. Prepare a periodic report (annually or as required) regarding the status of the chemical inventory and chemical waste of the department. first aid kits. Regularly check current loads of aircons. Together with a trained professional. Provide thesis students with chemical waste bottles. wood.

Wear clothing that would protect your body against contact with chemicals. e. Always wash hands. and experiments. or other acts of mischief. use medication. and how to dispose of chemical waste properly. The first thing you do in entering the lab is to put on your apron the last thing on leaving is to remove it. to the lab instructor. Contact lenses must not be worn. Although the Department aims to provide a safe working environment for its students. fire escape. drink. Consider lab work serious work. Come in the lab prepared. Keep personal belongings. not preschoolers. the student should be responsible for their personal safety when they are working in the lab.) Learn how to use instruments properly. Know the location of the fire extinguisher. and face before leaving the work area. c. Follow hazard precautions. Do not perform unauthorized work. Never eat.5 The Role of Students/Researchers The student/researcher is responsible for complying with the safety rules and operational procedures of the Chemistry Department. No pranks. e. Laboratory Operations & Safety Protocol 9 of 65 V1:2010 . c. Aprons or some suitable protection must be worn at all times in the laboratory. from the work area. whether major or minor. Never use your handkerchiefs or face/hand towels to wipe things in the lab. 2. Any deliberate behavior that may compromise you and your co-workers’ safety may be grounds for your dismissal from that lab class/course. having read the experimental procedure in advance. When you are organized. or the lab technician in charge. Goggles resting on your forehead are not in a position to protect your eyes. you are less likely to commit accidents. arms. 4. 6. c. first aid kit. preparations. Read and understand general safety procedures. how to dispense the correct amount of reagents properly. and treat all chemicals with respect. e. Safety glasses must be worn inside the laboratory. b. d. 5. or slippers. horseplay. You are university students. d. reactivity. (See Figures 1-5. Keep a neat and clean work area. 7. Research the hazards and physico-chemical properties of the chemicals used in the experiment. smoke. d. b. consult with your lab instructor/supervisor. especially with hazardous chemicals. Never work alone. Every student should: 1. research supervisor. Read and prepare for remedies in case of exposure. b. flammability. Wear proper personal protective equipment: a. or cellphones. Confine hair and loose clothing. This should be done even if gloves are used. 3. Follow protocols for handling chemicals and for dealing with spills. safety shower and eyewash. and toxicity. Wear clothing appropriate for work: Do not wear shorts. Be informed about experimental procedures and appropriate safety concerns: a. sandals. Avoid unnecessary exposure to chemicals: a. Report all accidents. books. or apply cosmetics inside the lab or storage areas.1. corrosiveness.g. If in doubt. and those specific to your experiment. such as bags.

rapidly spreading fire).Chapter 2 Emergency Protocols 2. Who do you call for assistance? If the incident is small and contained.3 (Fire Emergency). lab technician. or any Chemistry Dept. Gonzaga (local 5624) 5102) The University security personnel (local 4111. disaster-assistance team captain of the building. Alert others in the area of the accident. personnel (local 5624 or 5631) The Chemistry Department office (local 5620) 4111. 4112.g. etc. 4113 ) After office hours: • The University security personnel (local If the incident is out of control (e. 2. If the situation is beyond your control and presents a danger to you.1 Dealing with Emergency What to do: 1.) as above. evacuate the Note: For fires. immediately alert any of the following: • • • • The disaster-assistance team captain of the building (local The Fire Marshall at OAS. area and immediately call for help. Try to alleviate the immediate need in the situation without compromising your own safety: • • • Remove an incapacitated co-worker from the accident area Turn off fuel and power lines. 4112. immediately alert any of the following: During office hours: • • • Any faculty of the Chemistry Department. use fire extinguisher Immediately call for help. 3. especially the one in-charge of the lab The lab technician. 4113) The following fire departments: o o o o 8363) Marilag Fire Sub-station (913 0125/510 9299) Pinagkaisahan Fire Sub-station (474 2695) New Era Fire Sub-station (931 9894/931 4956) Central Fire station (928 • University personnel (faculty. follow evacuation procedures under Section 2. Laboratory Operations & Safety Protocol 10 of 65 V1:2010 .

and must be updated annually.2 Emergency Telephone Numbers Note: These numbers are subject to change. Luke’s Medical Center UP Health Service 372 3825 924 3601 925 2401 723 0301 981 8500 local 112 Laboratory Operations & Safety Protocol 11 of 65 V1:2010 . Fire Departments • • • Quezon City Marikina Mandaluyong 928 8363 933 3076 5322189 Ateneo de Manila University • • Trunk line Telephone Operators 426 6001 local 4199 Chemistry Department Directory • • • • Department Office (Chair/Secretary) Schmitt Hall Stockroom SEC Stockroom Faculty Offices 5620 local 5624 local 5631 local local 5621-23.2. University Gym) Gate 2 Gate 3 local 4111 local 4112 local 4113 local Office of Administrative Services • Fire Marshall (Gonzaga Hall) 5103 Physical Plant Office • • • • • Administrator Secretary Switchboard Operation Center (South Wing. 5625-33 University Security Office • • • Main Office (North Wing. University Gym) Direct Line local 4100 local 4101 local 4102 local 4104 426 5940 Infirmaries within Campus • • • • Health Service HealthDev (at the Social Development Complex) Jesuit Residence (ask for Infirmary) Loyola House of Studies (ask for Infirmary) local 5110 local 4630-33 local 3399 local 3650 Nearby Hospitals • • • • • Capitol Medical Center National Kidney Institute Philippine Heart Center St.

2. b. 2. 5. stay close to the floor.) 4. ovens. If the fire is small and confined to its origin. remember: Fire Extinguishers. Evacuation Procedures. “Gas” extinguishers. 1. 3. • • • • P – Pull the pin. If you encounter smoke. c. 5. Laboratory Operations & Safety Protocol 12 of 65 V1:2010 . Meet the fire department outside of the building and provide information about any special hazards and other considerations. Identify yourself Identify the kind of fire Identify the building and room number Give your telephone number Don’t hang up until the dispatcher has the needed information.g. Alert others in the area of the fire. WARNING: Do not attempt to fight a fire that is spreading rapidly or if you are not sure how to operate the fire extinguisher. The fire may block your exit. S – Sweep from side to side. offer first defense against flammable liquids or electrical fires without leaving a powder residue which could harm electronic equipment. Secure potential hazards in your area such as open flames. for example.3 Fire Emergency What to do: 1. d. Most chemical laboratory fire hazards require dry chemical multipurpose extinguishers (ABC) which must be installed in hallways. Provide the following information 3.) To use the fire extinguisher. Close the doors to confine the fire. small appliances. S – Squeeze the lever. e. 4. Immediately inform any of the following: • • • 5624) The Fire Marshall at OAS. 2. etc. and evacuate the building. compressed gasses. Do not re-enter the building unless you are given permission to do so. Gonzaga hall (local 5103 or 5118) Quezon City Fire Department (928 8363 or 928 6535) The disaster-assistance team captain of the building (local a. There are emergency exit routes posted on the wall of each room. (See Figure 4. e. Close windows and doors in your area. assume that the emergency is real until you are informed otherwise. use the portable fire extinguisher. Leave the building using the nearest stair tower to reach ground level. containing CO2. A – Aim the nozzle. and crawl if necessary. When alerted of a fire. (See Figure 5.

safety shower or eye wash fountain. If the material is not reactive to water. as appropriate.2. even of concentrated reagents. adding some water to provide solvent for the neutralizing reaction. has a low order of toxicity. Spill Clean-Up 1. Seek medical attention (Health Service. 5110). you can add water to the spillsand mixture to act as dust-suppressant. Mix neutralizing chemical into the spill. Use. Ventilate the spill area. Rotate the eyeballs so that all surfaces are rinsed. Forcibly hold the eyelids open as necessary. Acids and Bases: Avoid contact with skin. 6. 8. local 5624 or 5631). 3. 9. a minimum of 20-minute flushing with copious amounts of water is recommended. Do not brush up solid spills since this may produce airborne dusts. 4. 2. 7. sand. paper towels are not suitable for cleaning up flammable spills. These should be laundered separately from other clothing before reuse. Dispose them properly. Spills of Specific Types of Chemicals 1. Check for and remove contact lenses. However. and there is no fire hazard. and respiratory protection. If a volatile. Notify the faculty or lab technician in charge of the class (local 4. and turn off spark producing equipment. Exposed skin should be thoroughly washed with soap and water. Continue flushing with water for 15 minutes or more. 5. 3. when necessary by opening windows or use a fan. (See Spills of Specific Types of Chemicals below). Add sand to contain the solid spill. flammable or toxic material is spilled. Use pH paper to determine whether the acid or base have been neutralized. Report the incident to faculty or lab technician. gloves. If the material is not particularly volatile. For chemical splashes on the eyes. or an absorbent can be used to contain the spill. Immediate Action 1. When the toxicity of the spilled material is unknown. 7. Use sand/soil to contain liquid spills. closing the door and warning others not to enter the contaminated area. Shut down all equipment and vacate the area until it is decontaminated. treat the spill like a potential health hazard by avoiding exposure and seeking assistance from a trained personnel. Laboratory Operations & Safety Protocol 13 of 65 V1:2010 . proceed with clean-up operations. not highly corrosive. Avoid skin contact and minimize inhalation. 2. A quantity of solid not much greater than the volume of the liquid spilled should be sufficient. warn everyone immediately to extinguish flames. 5. especially for volatile and toxic spills. Do not leave paper towels or other materials used to clean up a spill in open trashcans in the work area. Use paper towels and dustpan to collect the spill residue. 6.4 Chemical Spill Dropping containers or otherwise spilling chemicals in the open lab can result in exposure to hazardous agents. For small liquid spills (<100 mL). Everyone should leave the affected area. Place in a labeled plastic bucket or container. use an absorbent material that will contain the liquids. paper towels. Wear appropriate personal protective equipment: goggles. Any contaminated clothing should be removed and containerized. respectively. and if applicable. Neutralize acids and bases with solid sodium bicarbonate and citric acid. It is important to minimize the extent and possible effects of exposure to these hazardous chemicals.

poison remedies. For more general concerns. Rotate the eyeballs so that all surfaces are rinsed. The situations described herein are the ones of particular concern in a chemistry laboratory. Keep victim warm. Heavy vapors (MW >>29) will lie along the floor. (See Figure 6.5 Escape of Noxious Gases Immediate Action 1. Modesty should not deter removal of clothing in a true emergency. Take (do not send) the victim to the Office of Health Services (local Sciences Building). 2. Check for and remove contact lenses. Quickly remove chemically soaked clothing. Very much lighter vapors will rise. burn ointments. 2. or by adding a mixture of finely powdered sodium thiosulfate (85 g) and powdered EDTA (15g). Laboratory Operations & Safety Protocol 14 of 65 V1:2010 . Final rinse is clear water. The stockroom must also be ready to provide first aid supplies: bandage. cutting or tearing them if necessary to get them off rapidly. Do not use sulfur to cover mercury since this will just complicate disposal. 2. Forcibly hold the eyelids open as necessary. etc. antiseptic wash. accordingly. Mercury spilled into floor cracks can be made non-volatile by amalgamation with zinc dust. In any event. Chemicals on the Body: Flush areas copiously with water under safety shower or under the sink. 5110.) Domestic vacuum cleaners must not be used.. thus increasing the damage to the skin and promoting poisoning through the skin.After neutralizing. Chemicals in the Eyes: A minimum of 20-minute flushing with copious amounts of water is recommended. Leave the room in either upright or crawling position. refer to a more extensive first aid manual. Place the source of gas in a hood (and make sure to turn the motor on). evacuate personnel. First Aid for Specific Types of Accidents 1. Solvents may cause the oil to dissolve in the skin. Mercury: Because of the high toxicity of mercury vapor. and the burning and poisoning through skin by HF gas. 2. Social 2. Watch victim carefully for shock. HF) with silica-containing materials such as sand or vermiculite. spilled mercury should be cleaned-up immediately and thoroughly using an aspirator or vacuum device.6 First Aid All members of the Chemistry Department must undergo a yearly first aid and cardiopulmonary resuscitation (CPR) course and should be ready to apply first aid measures to an injured person in a lab. mopping should follow. WARNING: A mask may protect the wearer against inhalation of noxious gases. WARNING: Do not clean-up hydrogen fluoride (hydrofluoric acid. and close off the room from the rest of the building. 3. etc. Contaminated materials used to clean up the spill should also be placed in properly-labeled containers. but be warned against absorption through the skin of such substances such as HCN gas. Water-insoluble oils must be washed off with soap and water. aniline vapor. Rinse mop and bucket. etc. WARNING: Do not use solvents to wash off offending oily chemicals. open windows.

3. Keep victim warm. extreme paleness of the face. WARNING: Application of cold to large areas of the body produces shock. Watch victim carefully for shock. Keep victim warm. heavy sweating. WARNING: Do not apply greasy burn ointment to chemical burns. The antidotes required. 5110. 5110. as 3% boric acid (remedy for base) or 5% bicarbonate (remedy for acid). Also refer to first aid wall charts. have shock victim lower head between knees. have him lie with head lower than the body. or better. trembling. Only very minor burns should then receive burn ointment. Take (do not send) the victim to the Office of Health Services (local Sciences Building). Keep moist with neutralizing solution soaked on gauzes. Remedies include: egg albumin. magnesium sulfate. Transporting the Injured Person: No one should be sent to seek medical attention without accompaniment. charcoal. mustard powder. even though at the time you dismiss them they assure you they feel fine and are quite ready to go alone. cold sweat. Shock: Accident victims experience shock. Keep moist. weakness of the arms and legs. Laboratory Operations & Safety Protocol 15 of 65 V1:2010 . Shock can be fatal! Be on the lookout for it. are usually printed in red letters on the bottle labels. Watch victim carefully for shock. Soak affected part in ice water for 20 minutes to reduce deep burning. shivering and chills. The cold shower will suffice. 2. extreme dullness or sluggishness. the injured person must be taken (not sent) directly to the Office of Health Services (local 5110. nausea. and first aid precautions to follow in case of chemical poisoning. Also. shallow rapid breathing. Take (do not send) the victim to the Office of Health Services (local Sciences Building). and difficulty in breathing. Take (do not send) the victim to the Office of Health Services (local Sciences Building). and tannic acid. Keep victim warm. To restore blood to the head. Social 2. pounding heart. Watch victim carefully for shock. jacket. All these are signs of trouble and require immediate medical attention. Burned areas should be cooled immediately. Social 4. and shallow breathing.). Chemical Poisoning: Get poison remedies/antidotes from the stockroom manager or lab technician. hence the advantage of using the safety shower. do not apply burn ointment.7 Medical Attention After First Aid After being administered first aid. Social Fire Burns: Rush victim under safety shower or smother fire on victim with a blanket (e. 5110. lab coat. etc. Symptoms of shock include: cold sweat. Social Sciences Building). nausea. Treatment with ice water is not recommended for large-area burns. a victim should not drive a car for several hours after a severe injury. Cover burned areas in 3% boric acid. Signs of Danger: Watch the victim for blood leaving the head (face turning white) or excess blood in the head (face turning red). On most fire burns. vomiting. Watch victim carefully for shock. It may be the most dangerous result of an accident.g. The supervisor (faculty/lab technician) or a co-worker must accompany the injured person in seeking medical treatment.Follow water-washing with the correct neutralizing solution. it seals the chemicals under the greasy coating. Keep victim warm. 1. There is always the possibility that they may collapse en route. etc. very rapid pulse. jittery talk.

2. Keep the victim warm. Only give inhalation (aromatic spirits of ammonia or amyl nitrate) in the mouth only if the victim is unconscious. WARNING: It is still best to take the victim to see a physician first before giving any oral remedy.WARNING: If the face is flushed or red. 1. to the building manager or lab technician so that the equipment can be recharged of replaced. apoplexy. be sure his air passages do not become blocked. It is the student’s/researcher’s responsibility to notify his supervisor immediately of any work-related injury or illness. 3. hospitalization. or medical treatment in excess of first aid will be investigated by the Chemistry Department to determine liability or the possible need for corrective action. Give oral stimulant (hot tea or coffee) if conscious. It is also the supervisor’s responsibility to file an injury/accident report with the Chemistry Department. etc. for establishing legal responsibility for an accident. abdominal injuries. Report the Use of Safety Equipment: Immediately report the use of eye wash. neutralizing chemicals. If he vomits. and not at all in case of fractured skull. apoplexy. a coworker should notify the supervisor as soon as possible. Accident reports (see Appendix A. 3. Laboratory Operations & Safety Protocol 16 of 65 V1:2010 . for establishing a claim for damages of insurance benefits. for demonstrating whether faculty/staff were negligent in preventing the accident or careless in subsequent treatment of the victim. fractured skull. fire extinguishers.8 Reporting Accidents and Injuries All personal injuries and accidents should be reported in order that corrective action may be taken to minimize the probability of recurrences. If the injured person is not able to do so.2) are of the utmost importance for correcting procedures. It is the supervisor’s (faculty’s) responsibility to ensure that the student receives prompt treatment of the injury by obtaining first aid or assistance to medical treatment. File injury/accident reports with the secretary of the Chemistry Department. or sunstroke. 2. showers. Do not give stimulant until bleeding is controlled. elevate the head rather than lower it! This may occur in the case of sunstroke. or hemorrhage of the head. Any injury resulting in lost work time.

Laboratory Operations & Safety Protocol 17 of 65 V1:2010 . The building is named after Fr. the Ateneo Chemistry Department has been able to maintain a good and effective working set-up which has yielded beneficial outcomes. This set-up is also based on as sense of trust that each one will do his share in ensuring these practices. William J. and its long tradition of high standards in safety. The heart of an effective working set-up is the responsibility and discipline of users of the building and its facilities. who was instrumental in its design and construction. finances.1 Schmitt Hall T he Chemistry Department is housed at Schmitt Hall (formerly the Chemistry Building). It has given us the edge on effective equipment maintenance because malfunctions were reported at once.Chapter 3 The Use of Building & Facilities 3. Over the years. with a laboratory extension for undergraduate labs on the 2nd floor of the 3rd wing of the Science Education Complex (SEC-C). which in the past have served the Department well. The judicious use of resources—energy. It has spared us from serious theft because security measures were taken in earnest and implemented. and effort—has led to the conservation of resources. While in some cases some convenience is sacrificed. you will see that the good of many outweighs individual convenience. Schmitt. are maintained to a high level of compliance. It has saved the Department from serious accidents because of the emphasis on safety at the workplace.

Service Rooms a. 2nd Floor SEC-C 307 309 6. Director’s Office Staff Office GC-MS Lab NMR Lab 102 104 103 105 5.116. c. also houses the AAS and HPLC 207.115. g. d. c. e. f. Stockroom Balance Room Instrumentation Room AFM Room Multimedia Room Glassblowing Workshop 208 308 310 204 111 4th Floor 4.2 Room Addresses 1. General Chemistry Lab Organic Chemistry Lab Analytical Chemistry Lab Physical Chemistry Lab. f. 2nd Floor SEC-C 209. Department Office Faculty Offices Faculty Lounge 112 101. b. d. e. c. 115.3. Research Laboratories a. b. d. c. c. b. Offices a. 205 2. Teaching Laboratories a. Organic Synthesis Lab Natural Products Lab NCIC Wet Lab Biochemistry Research Lab Polymer and Materials Lab Polymer/Biochemistry Wet Lab Electrochemistry Lab 203 202 206 304 302 305 303 Laboratory Operations & Safety Protocol 18 of 65 V1:2010 . d. Lecture/Seminar Rooms 3. 301 201 109. b. National Chemistry Instrumentation Center (NCIC) a. b.

polymer and materials. For research assistants and students doing research. a.). to ensure the safety of lab workers. to 10:00 p. aircon. Lights should be turned on only in the appropriate areas of work. d.. overnight stays are discouraged.m. Toilet and shower room should always be maintained in a clean. etc. Anyone sleeping in the faculty lounge should be up by 6:30 a. Bathroom tissue should not be removed from the toilet.m. During these times all doors should be unlocked for safety. However.3 Use of the Building 1. The building is opened to the public by the building manager by 7:00 a. and the Office of Administrative Services must be informed. glasses. b. At least two people should stay for overnight work. Try to work out a schedule that will go from 6:00 a. Researchers and graduate students may loan keys to the special labs (instrumentation.m. Laboratories should only be unlocked when there are lab classes or when an experiment has to be performed in that lab. N2. 3. ovens and other lab equipment (unless overnight run is explicitly allowed by senior faculty). He. they have the responsibility of seeing to it that the labs used are properly locked after use. faculty and staff can stay inside the building until 10:00 pm.m. should be cleaned. coffee maker. Never assume that someone else will do these for you. gas lines (LPG. lights. the opening and locking of rooms is the main responsibility of the faculty handling the class. Overnight stays in the building should be processed with the Department office: a. It is a basic rule in any good chemistry lab to have at least 2 people in the lab. This will allow the office to inform the administration and security. should be submitted to the secretary of the Chemistry Department at least 2 days before the event. and doors. 3. plates.m. A written request form (Appendix A. e. c. For classes. b. d. All used utensils. As a rule.4 Access Keys 1. b. Food supplies should be sealed and arranged properly. 2. Tables should be wiped clean. and the sub-master key (for labs). Always lock doors after every use. respectable condition. Only faculty members and the lab technicians have access to keys for the main door. The faculty lounge should be kept clean and orderly.1). 2. a. and locked by the utility man at 6:00 p. 4. Sign at the Night Log. Sink should be properly cleaned. etc. In case the building manager is not yet around by this time. The last person to leave a laboratory should make sure that the following are closed/locked/turned-off properly: windows. Anyone staying beyond 10:00 p. e. so that the entry or exit from the lab is easy in an emergency. a. should be responsible for obtaining permission and getting access to the main door. faculty or staff may open the main door and the classrooms. It is for your own safety. 5. faucets. but both the lender and Laboratory Operations & Safety Protocol 19 of 65 V1:2010 . their respective offices. If an experiment requires longer than 15 hours. c.m. natural products labs) from a faculty member. c. hoods. endorsed by the mentor/supervisor. b.3. overnight stay needs to be approved by the mentor and department chair.

Access keys are loaned to you in trust. 5. 3. and glassblowing workshop: a. 6. (The student’s name must be marked in the Instrument Authorization Table posted in the analytical chemistry bulletin board. and shut-down routines. warm-up. 3. they should take the responsibility of informing the faculty involved or the stockroom technicians when they are finished. Report immediately to the person-in-charge or to the Department office when instrument supplies are running low (e. 3. c. especially the power-up. All users must familiarize themselves with the operation of the instrument. Creating duplicates on your own is strictly prohibited. Verify the power requirements of the instruments (110 or 220V) before plugging it into the outlet and turning it on. they should be locked at all times.5 Special Rooms 1. Access/use of any instrument requires check-in and authorization by faculty-in-charge. the AFM room. chart paper. but keep them closed. Unlock the doors. etc. SHORT NOTES are provided as supplement to the manuals.6 Use of Instruments 1.g. Laboratory Operations & Safety Protocol 20 of 65 V1:2010 . so that no fatal mistakes are committed. This is needed to prevent further damage and to ensure speedy repair. ink. If no sign is placed or if the time is over. Keep the doors locked even when leaving the room for a short while. 10-15 min). b. 3. turn on the aircon. When rooms are unoccupied. A student cannot use the instrument alone unless he/she is authorized to use that instrument. 4. Most major instruments have voltage regulators. 3. When using the polymer and materials lab. 2. These cannot be moved/relocated without the explicit approval of the Department Chair. If students are using the room and do not have easy access to the key. Equipment and accessories used should be kept in proper order. The performance of the balances are dependent on temperature. If they are going to leave the room for a short period of time (e. the research labs. anyone with a key to the room should lock it and report the matter to the Department office. Review the instructions before the operation. either or both the aircon or the dehumidifier must be turned on (for the FTIR and the AFM).g. Do not unplug them from their voltage regulators. Do not attempt to fix the equipment yourself. 4. without explicit approval of the Chairperson and a senior faculty member. Understand the instrument thoroughly. Faculty offices should never be left unlocked. Report any noticeable malfunction of the instrument immediately by reporting it to the Department office. no equipment (major nor minor) can be taken out of the building. Please be considerate of faculty members who hold offices in some of these special labs. the physical chemistry lab. 2. As a rule.the one who borrowed are accountable for the keys. Return the items to where they were found.).)All users must sign the instrument logbook. and there are no other people using the room. Keys should never be left lying around in the rooms. and AFM room. indicating the time they are returning. Any loss of keys should be reported immediately to the Department office. This assumes responsibility in the use of the keys. 4. When using the balance room. When using the instrumentation room. they have to put up a sign on the door.

comments) if you are leaving something (e. Only students who passed the safety exam will be allowed to use the instrument. Instrument Parts c. instrument use has priority over other use. Procedure for instrument check-out 1. Instrument parts b. analysis. The student who wishes to use the instrument must fill-up the instrument authorization form (Form A. For the commonly-used instruments (UV-Vis. 2. a general check-in will be scheduled every July of each year. 2. The student must already have actual samples to run during check-in. After the last use of the instrument. Working environment 3. If the student will be using the instrument only once or twice. Safety and Precautionary Measures d. make sure he/she understands that you are leaving and you are turning over the responsibility of shutting down to him/her. Instrument accessories c. He/She can get a copy of the form from the instrument manager. 9. The student schedules the instrument check-in wth the faculty-in-charge one week before the actual check-in. When done. For computers connected to the instrument. the student must schedule an instrument check-out with the faculty-in-charge. the faculty-in-charge must discuss the following to the student: a. Leave a note (name.7. The Faculty-in-charge signs the certificate of authorization if the student has demonstrated competency in using the instrument during the next sessions. All graduating students must make sure that they have checked-out of the instruments they are authorized to use for clearance purposes. Instrument General Working Principles b. students who wish to use it must request the technician or a faculty member to open the lab for them. 3. time.g.3). If the instrument room is locked. Only students who will be using the instrument more frequently will be checked-in. The student and faculty-in-charge schedules the next session. The student submits to the instrument manager for filing and documentation the instrument authorization form filled up to the certificate of authorization part. 8. Consumables d. 5. water bath. computer) turned on. Procedure for Instrument Check-in 1. AAS and IR spectrophotometers). During the check-in. instrument. Short Notes of the Procedure e. The student’s mentor must approve the student’s plan to check-in. Laboratory Operations & Safety Protocol 21 of 65 V1:2010 . 6. Important Reminders and Considerations 4. date. do proper shutdown. Equipment that is left on without any note will be shutdown after 6:00 pm. 10. he/she can ask a person who is already authorized to use the instrument to analyze his/her samples. The Instrument manager updates his list of students authorized to use the instrument. The faculty-in-charge signs the check-in part of the instrument authorization form as proof of check-in. oven. ON TH SPOT checking-in is not allowed. The date of authorization must be different from the date of check-in. The faculty-in-charge inspects the following during check-out: a. If someone is using it. Lock the lab door immediately.

Third Offense: One week ban in using the laboratory. the department chairperson discusses with the requesting party the department policies on the use of instrument and the charges: a. The person writes a letter addressed to the department chairperson requesting to use an instrument. If the requesting party agreed to the terms and conditions. Procedure for instrument use by outside parties 1. 3. Laboratory Operations & Safety Protocol 22 of 65 V1:2010 . He/She must include in the letter the purpose of the instrument use. Operator fee 4. 2. Second Offense: Oral reprimand and community service. If the request is granted. Mentor will be notified. Mentor will be notified. the department chairperson assigns to the available faculty-in-charge the granted requests. He/She must include as an attachment the details of the procedure and instrument conditions. feasibility of the analysis and availability of the needed accessories. The department chairperson together with the faculty-in-charge and/or the instrument manager decides whether to grant the request or not based on the instrument schedule. Instrument use and b. Mentor will do necessary action.Penalties Any student who fails to follow the policies and guidelines will be subject to the following penalties: First Offense: Oral reprimand from the instrument manager and the department chair.

Work areas are to be kept clean. security guards may be asked to check on the lab worker. ice chests. Wastes are to be deposited in appropriately labeled receptacles. and plans should be made to avoid hazards in case of failure. Coffee. The safety. Standard signs and symbols have been established for a number of special situations. Lab refrigerators. must be maintained free from obstructions.and hazard-sign systems in the lab should enable a person unfamiliar with the usual routine of the lab to escape in a emergency (or help combat it. smoking materials. such as fire hazards (See Figure 8). water. safety performance inevitably deteriorates. The supervisor has the responsibility for determining whether the work requires special safety precautions.5 Warning Signs and Labels Laboratory areas that have special or unusual hazards must be posted with warning signs. Temporary holding containers should be clearly marked. In any case. 4. Alternatively. Drinking. and controls. snacks and lunches are not to be brought into laboratory areas. unless it is explicitly allowed by the Chair and senior faculty. it is prudent to avoid working in the laboratory alone. Food should be stored. Glassware and utensils that have been used for lab operation are not to be used for food or beverages. eyewash stations. Operations should be designed to be safe. drink. and inert gas. and consumed in an area free of hazardous substances. Access to exits. etc. and chemicals and equipment must be properly labeled and stored. soft drinks. Likewise.2 Working Alone Generally. Experiments known to be hazardous should not be undertaken by a worker who is alone in a laboratory. Laboratory Operations & Safety Protocol 23 of 65 V1:2010 . 4. Never leave a set-up unattended overnight. Cleanup should follow the completion of any operation of at the end of each day. and cosmetics is a potential route for exposure to toxic substances. are likewise not to be used for food storage. handled.1 Overnight Operations If laboratory operations are carried continuously or overnight. Extinguishers are to be labeled to show the type of fire for which they are intended. Chemicals that are no longer needed should not be permitted to accumulate in the lab. Other signs should be posted to show the locations of safety showers. biological hazards. 4. it is essential to plan for interruptions in utility services such as electricity. Under normal working conditions. Stairways and hallways should not be used as storage areas. the laboratory lights should be left on and an appropriate sign should be placed on the door or near the set-up. and fire extinguishers.Chapter 4 General Safety Principles 4. if appropriate). 4. and cold rooms. When housekeeping standards fall. Waste containers must be labeled for the type of waste for which they are intended.3 Eating. emergency equipment. laser operations. such as having two persons in the same room during a particular operation.4 Housekeeping There is a definite relationship between safety performance and orderliness in the laboratory. arrangements should be made between individuals working in separate laboratories outside working hours to crosscheck periodically. and Smoking Contamination of food. chemicals should not be brought into designated eating areas. exits.

an apparatus that is a closed system unless it is designed and tested to withstand pressure.6 Shielding for Safety Safety shielding is to be used for any operation having the potential for explosion such as 1. The pressure regulators must be removed and valve protection covers replaced before moving cylinders. 4. Although oxygen is quite safe under normal temperatures and pressures. Whenever operations are carried out under non-ambient conditions. 2. or contamination. grease. A cylinder connected to a piece of equipment and properly supported is considered to be in use. is prohibited. Pressure regulators and gauges must be compatible with the cylinder valves.8 Systems Under Pressure Reactions should never be carried out in. 2. elevated temperatures and/or pressures. Gas cylinders must be used in an upright position and clamped securely at all times. graphite. Acceptable methods of support include: wall-mounted or bench-mounted gas cylinder brackets. if desired. chains or belts anchored to walls or benches (see Figure 9 in the Appendix). Laboratory Operations & Safety Protocol 24 of 65 V1:2010 . Compressed gas cylinders must be supported and firmly restrained at all times. cylinders weighing less than 50 lbs may be carried.7 Compressed Gases The following rules summarize a few of the basic guidelines for the use and storage of compressed gases: 1. Whenever a reaction is attempted for the first time (small quantities of reactants should be used to minimize the hazards). Pressurized apparatus should have an appropriate relief device. 4. due to the oil residue. Gas cylinders must have the valve protection cover in place (see Figure 9 in the Appendix) except when in use.g. nor heat applied to. Moving by spinning. or any other oxidizable substance. Shields are to be placed so that all personnel in the area are protected from hazard. 5-10 times more material). gauges. 7. All oxygen valves. whether full or empty. For movement within shops and laboratories. may result in the rapid and violent oxidation of normally non-reactive materials.e. 8. sliding. Whenever a familiar reaction is carried out on a larger than usual scale (e. For example. etc. If the reaction cannot be opened directly to the air. 6. pipes. i. regulators. an inert gas purge and bubbler system should be used to avoid pressure build up.. Appropriate first aid and antidote information and supplies must be provided and clearly marked at room entrances. 3. and 3. a regulator for oil-pumped nitrogen could produce a serious explosion if subsequently used for oxygen. even though the cylinders are secured to a dolly. Smoking is not permitted in the area where flammable gases are used or stored. 4.4. the use of adapters is prohibited. rolling. Appropriate dollies are to be used to move cylinders weighing more than 50 lbs. Toxic and poisonous gases must be used only in fume hoods or other enclosures vented directly outdoors. and fittings must be scrupulously free of oil. 5.

(For example. Equipment such as Dewar flasks should be taped or shielded. The use of plastic or metal connectors should be considered. Vacuum-jacketed glass apparatus should be handled with extreme care to prevent implosions. Wetting the glassware should also facilitate the process of inserting glass tubing into rubber stoppers. Only glassware designed for vacuum work should be used for that purpose. Careful handling and storage procedures should be used to avoid breaking glassware. Neither liquid nitrogen nor liquid air should be used to cool a flammable mixture in the presence of air because oxygen can condense from the air.9 Cold Traps and Cryogenic Hazards The primary hazard of cryogenic materials is their extreme coldness. Adequate hand protection should be used when inserting glass tubing into rubber stoppers or corks or when placing rubber tubing on glass hose connections. Dry ice should be added slowly to a liquid portion of the cooling bath to avoid foaming over. Insulated gloves and a face shield may be needed when preparing or using some cold baths. Glass-blowing operations should not be attempted unless proper annealing facilities are available. which can represent unusual risks for the first-time user. can cause severe burns if allowed to contact the skin. which leads to an explosion hazard. Tubing should be fire polished or rounded and lubricated. Workers should avoid lowering their head into a dry ice chest: carbon dioxide is heavier than air. and surfaces they cool. (Small pieces should be swept up with a brush into a dustpan.10 Glassware Accidents involving glassware are a leading cause of lab injuries.) Glassware which is to be heated should be Pyrex or a similar heat-treated type. They. separatory funnels containing volatile solvents can develop considerable pressure during use. and hands should be held close together to limit movement of glass should fracture occur.) Broken glassware should be disposed of in a special container marked BROKEN GLASS. Appropriate insulated gloves should be used when handling dry ice.4. and suffocation can result. Do not store in a refrigerator or freezer. Hand protection should be used when picking up broken glass. Proper instruction should be provided in the use of glass equipment designed for specialized tasks. 4. Laboratory Operations & Safety Protocol 25 of 65 V1:2010 .

accurate inventory. IUPAC name. CAS number. chemical movement) can be exported as comma-separated version (CSV) files which can be opened in Microsoft Excel. Reports are easily prepared using the system’s Reporting Service 5. 2. PDEA and PNP.ateneo.e classification of wastes. Open Office Spreadsheet or any text-capable reader 6. consumption. Level 1 users may: 1. generation of reports (i. Scanner-server synchronization Chemical data in the barcode scanner are easily synchronized with data in the server 8. chemical Using the CMIS.e. efficient purchasing. Tracking of controlled chemicals under the EMB. Supplier details and hazard codes) Laboratory Operations & Safety Protocol 26 of 65 V1:2010 . 3. management and compliance with various laws. students) No accounts are assigned to Level 1 users. Different user-levels Only selected accounts can access restricted areas of the system. The system may be accessed by any computer within the campus (http://cmis. chemical waste disposal and chemical waste movement) User Levels CMIS has four (4) user-levels (Table 1). Safety Data Sheets (SDS) bank Safety Data Sheets (SDS) for chemicals are stored in the system.1 Chemical Management and Inventory System (CMIS) The Chemical Management and Inventory System (CMIS) combines a web-based inventory system and barcode scanner to provide a means for efficient updating and query of chemical stock levels. stock-level. Check availability of chemicals in the department 2. Each chemical bottle is provided with a tear. The system of tracking is container-based. Obtain chemical information on chemicals (i. Back-up and Recovery Mechanisms CMIS has features which provide means to back-up the database and restore all data should the system fail 7. PDEA and PNP System not only allows monitoring of chemicals on-stock but also controlled chemicals under the EMB. threshold reports. Features 1. which provide quick access to: ü Physico-chemical information ü Handling and storage conditions ü Health and safety information ü Disposal considerations 4.and chemical resistant barcode sticker. Access to the different features depends on the user-levels as shown in below: User Level 1 (Guests. Hazardous Waste Inventory System The CMIS is linked to the Hazardous Waste Inventory System (HWIS) which allows monitoring of chemical waste generation and disposal. CMIS is meant to address the need for accessibility of information.Chapter 5 Chemical Management 5. Exportable Reports Reports (i.e. Information database Supplier and chemical information are stored in the database.

A page will appear where the Chemical Name or IUPAC Name or CAS Number of the chemical can be entered. To check for stock levels of chemicals using the Inventory System. 2. In addition to the features available to a level 1 user. Input the reference number issued upon checked out from the Request Wizard. click on “I do not need any more items. Access (Material) Safety Data Sheets ((M)SDSs) of chemicals 4. If request has been approved. 2. 4. click on the “Click here to View Existing Chemical Request” 2. 6. The system will then provide the requestor with an update on the status of his/her request. Please take me to check out. Level 2 users also have access to information regarding the stock level and stock location of the chemicals. List of requested items may also be viewed by clicking on “Click here to review requested items. Checking status of request 1.” The check-out page will then show the reference number which can used by the requestor to review status of request. Provide the chemical’s common name. the system will then ask for the following: Concentration – what is the concentration of the chemical that you need? Grade – what is the grade of the chemical? How much do you need? – Input amount is in liters or grams. To Request for Chemicals. If the chemical is on stock. Show the signed form to the stockroom personnel and claim chemicals. Any special instructions for this particular chemical? – Special instruction on how the chemical is to be handled or stored should be noted here. Requests for other chemicals can be added to current request by clicking on “Click here to add more chemical requests. Click on the Chemical’s Common Name link to show the information about the chemical. 3. The Request Home Page will then be shown. Requestor Name – the name of the requesting party (who made the request?) Requestor Department / Company – the department or company affiliation of the requestor Purpose – the reason for the request (where will the chemical be used for?) Request Date – date of the request (when was the request made?) 4. click on the “Request” Link from the Home Page. Users may change the password using the Password Management feature of the system. To check the status of chemical requests.” 7. To end request wizard. Research Assistants (RAs) and junior faculty) An account and temporary password is given to Level 2 users. Stock Inquiry 1. click on Stock Inquiry. Proceed by clicking on the “Click Here to Request for a Chemical” link. Laboratory Operations & Safety Protocol 27 of 65 V1:2010 . 3. Request for a chemical Steps in requesting for a chemical (See Figure 11 in the Appendix for the overview) 1. 3. Fill up the form with the required information to proceed. The system will then ask for the chemical’s common name.3. User Level 2 (Teaching Assistants (TAs). 5. print the form and have it signed by your thesis mentor.” 8.

encode additional data (e. 1. In addition to the features available to a level 3 user. click on the link ”Back-up and Recovery” link on the Administrator Functions Section. click on the “add ____” link to display the form where new entries can be supplied. User Level 4 (Dean. Suppliers. technicianin-charge of CMIS) An account and temporary password is also given to Level 4 users. add/update chemical bottle inputs. Senior Faculty) An account and temporary password is also given to Level 3 users. use. storage. supplier information.User Level 3 (Department secretary. storage locations. right-click on the “The Back-up file” link and choose “Save Link As…” or “save Target As…” Synchronization of server and barcode scanner The sync station software is used to synchronize the barcode reader’s database with the server’s database.2 Controlled Chemicals In 2004. rules and regulations covering a broad range of activities related to the purchase. Users may change the password using the Password Management feature of the system. chemical grade. Place the scanner in the dock and wait for “active sync” to finish synchronization. Click “add ____. and disposal of chemicals. Then click the link. storage and disposal of the following controlled chemicals: Laboratory Operations & Safety Protocol 28 of 65 V1:2010 .g. Setting up of additional data to the CMIS 1. movement.e. Department Chair. The safety officer together with the system administrator of the MIS office is in-charge of the maintenance of the database system. Laboratory managers. In addition to the features available to a level 2 user.e. chemicals and thresholds) on the home page. chemical brands. A “Back-up succeeded” message will then appear on the screen.” button to submit and save new data. “Back-up. To synchronize the server and barcode scanner. thresholds etc. 2. close all active application in the barcode scanner. Sub-pages for the selected field will then be displayed. PNP and PDEA) monitor the purchase. CMIS manager. Click on the “Synchronize All” button to perform all operations in sequence. The system will then display a dialog box once all operations are finished. To back-up the databse. 3. hazard codes. 4. Different implementing agencies (i. dangerous drugs and explosives (Tables 2 and 3 in the Appendix). Level 4 users also have access to the hazardous waste inventory system and the administrative functions of the system.” 2. consumption. 2. chemical brand. new storage locations. 5. Level 3 users also have access to the reporting services of the system. preparation of a back-up of the database and synchronization of the server and barcode scanner. This includes management of CMIS accounts. storage locations. Open the Sync Station software and ensure that all databases are connected. These can be grouped under the general headings of environment. the government has started to implement various laws. 5. To save the back-up file. To add new data. Preparing a back-up of the database 1. Lab technicians. waste management. hazard codes and threshold levels may be done by clicking on the appropriate links (i. Level 4 users have access to all of the features of the CMIS. EMB. Setting up of additional data such as supplier information.). Users may change the password using the Password Management feature of the system.

Monthly consumption reports are also submitted to PNP. upon presenting the official receipt from the Cashier. delivery. possession or use of the following prohibited and regulated drugs and precursor chemicals (See Table 4 in the Appendix) Since the department uses some of these compounds for laboratory classes and research. distribution. Laboratory Operations & Safety Protocol 29 of 65 V1:2010 . Semiannual reports on the purchase and consumption of PDEA-controlled chemicals are also submitted to PDEA. sale. Since the department uses these compounds for laboratory classes and research. Procedure for requesting for controlled chemicals by outside parties 1. The person checks availability of chemical using the CMIS. A separate “Permit to Purchase and Move Explosive and Explosive Ingredients” should also be obtained from PNP prior to purchase of nitrates and chlorates. the department chairperson approves the request. from their importation. The department chairperson together with the faculty-in-charge and/or the person in-charge of controlled chemicals decides whether to grant the request or not based on the amount of chemical on stock. The university is required to register its possession and use of Mercury and Cyanide compounds. use. the person then writes a letter addressed to the department chairperson requesting to purchase the chemical. 4. Requesting party proceeds to the Cashier to pay for the chemicals being purchased. The university is required to obtain a Purchaser’s license from PDEA. 3. sale. Since these chemicals are also used in the teaching laboratories. 2. cyanide and their compounds. If the chemical is available.EMB The EMB regulates mercury. administration. If the request is granted. the university is required to obtain a “License to Possess Explosive and Explosive Ingredients” from PNP. delivery. administration. possession or use of explosives and explosive ingredients such as nitrates and chlorates. transport and disposal. manufacture. PDEA PDEA monitors and regulates the importation. distribution. Requesting party may then pick-up the requested chemicals from the stockroom. He/She must include in the letter where the chemical will be used as well as the amount of the chemical being requested. This license is renewed yearly. transportation. transportation. PNP PNP monitors and regulates the importation.

Highly toxic: (a) a chemical that has a median lethal dose (LD50) of 50 mg/kg body weight when administered orally to albino rats weighing 200-300 g each. Corrosive: a chemical that causes visible destruction of. Sensitizer: a chemical that causes a substantial proportion of exposed people or animals to develop an allergic reaction in normal tissue after repeated exposure to the chemical. and agents which damage the lungs. such as chloroform.1 Hazardous Chemicals All chemicals have toxic effects at some dose level for some route of exposure. when administered by continuous inhalation for one hour (or less if death occurs within one hour) to albino rats weighing between 200-300 g each. Hazardous chemical means any chemical which is capable of causing harm to people and the environment. Toxic effects are also classified as acute or chronic.000 ppm by volume or less of gas or vapor. Carcinogen: a chemical agent that causes a malignant disease or increases statistically the risk of cancer. hepatotoxins. (b) a chemical that has a medial lethal dose (LD50) of 200 mg/kg but not more than 1. when administered by continuous inhalation for one hour (or less if death occurs within one hour) to albino rats weighing between 200-300 g each. 7. (c) a chemical that has a median lethal concentration (LC50) in air of 200 ppm by volume or less of gas or vapor. Chemicals can have local or systemic effects.000 mg/kg body weight when administered by continuous contact for 24 hrs (or less if death occurs within 24 hrs) with the bare skin of albino rabbits weighing between 2-3 kg each. Chronic effects result from long-term exposure or appear after a latency period. corrosives. living tissue by chemical action at the site of contact. carbon tetrachloride. It is therefore wise to minimize exposure to chemicals. and o-toluidine. 1. sensitizers. which is not corrosive. whether by initiating or promoting it. (c) a chemical that has a median lethal concentration (LC50) in air of 200 ppm but not more than 2. or mucous membranes. Some of these compounds are fairly common materials used in many laboratories. liver enlargement. irritants. Local toxicity refers to the direct action of chemicals at the point of contact. 2. thiourea. Health hazard means a chemical for which there is statistically significant evidence based on at least one study conducted in accordance with established scientific principles that acute or chronic health effects may occur in exposed personnel. fume. Irritant: a chemical. Chemicals: carbon tetrachloride. agents which act on the hematopoietic system. or 2 mg/L or less of mist. It includes chemicals which are carcinogens. 5. skin. fume. eyes. 6. or dust. dioxane. neurotoxins. hydrazine. 4. benzene. nitrosamines. Signs & symptoms: jaundice. benzene Laboratory Operations & Safety Protocol 30 of 65 V1:2010 . Targeted Organ Effects a. toxic or highly toxic agents. or irreversible alterations in. Systemic toxicity occurs when the chemical agent is absorbed into the bloodstream and distributed throughout the body. (b) a chemical that has a medial lethal dose (LD50) of 200 mg/kg body weight when administered by continuous contact for 24 hrs (or less if death occurs within 24 hrs) with the bare skin of albino rabbits weighing between 2-3 kg each. Acute effects are observed shortly after exposure. reproductive toxins.Chapter 6 Handling Chemicals 6. Toxic: (a) a chemical that has a median lethal dose (LD50) of 50 mg/kg but not more than 500 mg/kg body weight when administered orally to albino rats weighing 200-300 g each. but causes reversible inflammatory effect on living tissue by chemical action at the site of contact. affecting one or more organs. nephrotoxins. or dust. or 2 mg/L or less of mist. 3. Hepatotoxins: chemicals which produces liver damage.

3 oC. acids. c.45. proteinuria. asbestos. Signs & symptoms: conjunctivitis. Chemicals: silica. flammable: any liquid having a flashpoint below 37. 2. Agents which act on the blood or hematopoietic system: decreases hemoglobin function. except any mixture having components with flashpoints of 37.1 oC. the total of which make up 99% or more of the total volume of the mixture.2 oC. absorption of moisture. yields a flame projection exceeding 18 inches at full valve opening. carbon disulfide. Signs & symptoms: narcosis. Explosive: a chemical that causes sudden. an oxidizer. loss of consciousness. a compressed gas. gas. c. pressure.3 oC. Physical hazard means a chemical for which there is scientifically valid evidence that it is a combustible liquid. Combustible liquid: any liquid having a flashpoint at or above 37. except any mixture having components with flashpoints of 93. Laboratory Operations & Safety Protocol 31 of 65 V1:2010 . f. Signs & symptoms: cough. Eye hazards: chemicals which affect the eye or visual capacity. Signs & symptoms: birth defects. Gas. Note: flashpoint means the minimum temperature at which a liquid gives off a vapor in sufficient concentration to ignite when tested according to ASTM D 56-79. but below 93. rashes.8 oC or higher. flammable: (a) a gas that.b. decrease in motor functions. or (b) a gas that. at ambient pressure and temperature. Compressed gas: (a) a gas or mixture of gases having. an absolute pressure exceeding 40 psi at 21. ASTM D 93-79. Chemicals: lead. almost instantaneous release of pressure. at an ambient temperature and pressure. spontaneous chemical change. when tested by the method described in 16 CFR b. or retained heat from manufacturing or processing. in a container. the total volume of which make up 99% or more of the total volume of the mixture. cyanides. deprive the body tissues of oxygen. Agents which damage the lung: chemicals which irritate or damage pulmonary tissue. Signs & symptoms: cyanosis. explosive. shortness of breath. (b) a gas or mixture of gases having. or higher. Neurotoxins: chemicals which produce their primary toxic effect on the nervous system. behavioral changes. g. forms a flammable mixture with air at a concentration of 13% by volume or less. Cutaneous hazards: chemicals which affect the dermal layer of the body. unstable (reactive) or water-reactive. e. Signs & symptoms: defatting of the skin. Signs and symptoms: edema. d. an absolute pressure exceeding 104 psi at 54. tightness of chest. Chemicals: carbon monoxide. h. Chemicals: mercury. or which can be ignited readily and when ignited burns vigorously and persistently as to create a serious hazard. or (c) a liquid having a vapor pressure exceeding 40 psi at 37. Reproductive toxins: chemicals which affect the reproductive capabilities including chromosomal damage (mutations). or high temperature. in a container. Chemicals: organic solvents. corneal damage. 1. and ASTM D 3278-78. that is liable to cause fire through friction. DBCP. Flammable: a chemical that falls into the following categories: a. Aerosol. and irritation. pyrophoric. A chemical shall be considered to be a flammable solid if. sterility. regardless of the pressure at 21. and heat when subjected to sudden shock. 3.4 oC. or a flashback (a flame extending back to the valve) at any degree of valve opening. Chemicals: ketones. Liquid. 4.8oC as determined by ASTM D-323-72.8 oC. flammable: an aerosol that. Solid flammable: a solid other than a blasting agent or explosive. chlorinated compounds. when tested by the method described in 16 CFR 1500. forms a range of flammable mixtures with air wider than 12% by volume. flammable. d. Nephrotoxins: chemicals which produce kidney damage. uranium.8 oC. Chemicals: halogenated hydrocarbons. regardless of the lower limit. and effects on fetuses (teratogenesis). an organic peroxide.

8. 5. 2.44. Laboratory chemicals in the form of dusts and particulates can become airborne when transferred from one container to another. its solubility in tissue fluids. condense. or will become self-reactive under conditions of shocks. For many operations it is not obvious that an aerosol is being generated and laboratory personnel may not be aware that a hazardous situation exist. laboratory personnel may unconsciously contaminate themselves when they touch work surfaces. Organic peroxide: an organic compound that contains the bivalent —O-O— structure. and which may be considered to be a structural derivative of hydrogen peroxide. which facilitate the absorption of chemicals into the body. eyes. its concentration. sores. sweat glands. 6. thereby causing fire either of itself or through the release of oxygen of other gases. where one or both of the hydrogen atoms has been replaced by an organic radical. throat. Actually. that initiates or promotes combustion in other materials. and lungs and can seriously damage these tissues. Spills and splash can result in overt contamination of the skin. a number of materials are absorbed through the skin to produce systemic poisoning. Many of the particulates generated during such procedures do not settle out but remain suspended in the air and are carried about by air currents in the room. and removing caps and stoppers.2 Routes of Exposure Dermal Contact. Inhaled gases or vapors may pass rapidly into the capillaries of the lungs and be carried into the circulatory system. or as produced or transported. sebaceous glands. decompose. The main portals of entry for chemicals through the skin are the hair follicles. by local action. However. injections with a needle and syringe. Chemicals can also gain entrance into the body when contaminated hands touch the mouth. Water reactive: a chemical that reacts with water to release a gas that is either flammable or presents a health hazard. pipeting. or dusts can produce poisoning by absorption through the mucous membrane of the mouth. 1. Such operations include weighing. Inhalation of toxic vapors. it ignites and burns with a self-sustained flame at a rate greater than one-tenth of an inch per second along its major axis. and cuts or abrasions of the outer layers of the skin. pouring.4 oC or below. Oxidizer: a chemical other than a blasting agent or explosive. 7. gases. Pyrophoric: a chemical that will ignite spontaneously in air at a temperature of 54. The degree of injury resulting from inhalation of toxic substances depends on the toxicity of the material. but solids and non-volatile liquids can also present an inhalation hazard for laboratory personnel. 3. One of the most frequent exposure to chemicals is by contact with the skin. Some of these particulates are capable of being inhaled and deposited in the respiratory tract. Laboratory Operations & Safety Protocol 32 of 65 V1:2010 . A common result of skin contact is localized irritation or dermatitis. pressure or temperature. will vigorously polymerize. Grinding and crushing procedures can also produce aerosols. Alert laboratory personnel will take care not to create unnecessary aerosols. stirring. nose. mists. 9. Unstable (reactive): a chemical which in the pure state. Also. or cuts. 6. glassware.1500. Inhalation. all laboratory operations involving an open vessel will result in the release of an aerosol. The follicles and glands are supplied with blood vessels. Splash created from spills and during vigorous shaking and mixing also results in aerosol formation. or equipment which become contaminated curing experimental activity. Inhalation hazards are often associated with gases and volatile chemicals. and the duration of exposure.

Furthermore. The American Chemical Society’s Committee on Chemical Safety states in Safety in Academic Chemistry Laboratories that. 2. Side shields that attach to regular safety spectacles offer some protection from objects that approach from the side but do not provide adequate protection from splashes. and visitors in laboratories where chemicals are stored or handled. For example. A face shield or mask may be needed when a vacuum system (which may implode) is used or when a reaction that has a potential for mild explosions is conducted. passed flammability tests. goggles should be worn when working with glassware under reduced or elevated pressure and when glass apparatus is used in combustion or other high-temperature operations. faculty. It is the responsibility of the lab supervisor to determine the level of eye protection required and to enforce eye-protection rules. They are intended for wear when there is danger of splashing chemicals or flying particles. Full-face shields that protect the face and throat should always be worn when maximum protection from flying particles and harmful liquids is needed. Also. 4. Three dimensions that are important in providing a comfortable fit include: temple length. Ocular Exposure. Eye and protective devices must be worn by students. The needle and syringe is one of the most hazardous items used in the laboratory. Other eye protection should be worn when a significant splash hazard exists. For full protection. Goggles: Goggles are not intended for general use. welding. The practice of mouth pipeting can result in aspiration of toxic materials. The eyes are of particular concern because they are so sensitive to irritants. when the work environment entails exposure to chemical fumes. In addition. safety glasses should be worn with face shields. more than two hours per day). Impact-protection goggles have screened areas on the sides to provide ventilation and reduce fogging of the lens and do not offer full protection against chemical splashes. Contact Lenses: Contact lenses do not provide eye protection in the industrial sense. or splashes. identification of contact lens wearers should be ensured for appropriate emergency care and for protection in work areas hazardous to the eyes. Injection. Ocular exposure can occur via splash or when contaminated hands rub the eyes. containers of toxic chemicals may break resulting in hazard from contact with broken contaminated glass. Accidents involving needles and syringes can results in injection contamination through the skin. 3. contact lens use should be restricted. or highly particulate atmosphere.Ingestion.3 Protective Clothing and Equipment Eye and Face Protection. molten metals. Few substances are innocuous in contact with the eyes and a considerable number are capable of causing burns and provide for rapid absorption of many chemicals. Safety glasses: Ordinary prescription glasses do not provide adequate protection from injury to the eyes. impact resistance. vapor. Food items and utensils themselves can become contaminated when stored in the laboratory. 6. The type of safety device required will depend on the nature of the hazard and the frequency with which it is encountered. Face Shields: Goggles offer little protection to the face and neck. Ingestion of toxic materials in the laboratory can also occur when contaminated hands come in contact with the mouth of with food items which are placed in the mouth. Splash goggles (“acid goggles”) that have splash –proof sides should be used when protection from harmful chemical splash is needed. nose bridge width. such as lasers and ultraviolet light. intense heat. “Contact lenses should not be worn in the chemistry laboratory Laboratory Operations & Safety Protocol 33 of 65 V1:2010 . These should have: a minimum thickness of 3 mm. Safety spectacles are recommended for those who require eye protection frequently and/or for long durations (e. and lens diameter. and intense light sources. and must be worn only in conjunction with approved safety eyewear. The minimum acceptable eye protection requires the use of hardened-glass or plastic safety spectacles. staff. 1.g. There are specific goggles and masks for glassblowing. and lens-retaining frames.

Cotton (pure or 60:40 cotton-polyester). and butyl and natural rubbers. polyvinyl chloride. Chemical spills on leather clothing or accessories (watchbands. and for similar operations where protection from chemicals is not needed. etc. Various synthetic materials such as Nomex and Kevlar can be used briefly up to 538 oC. Lab workers should know the appropriate techniques for removing protective apparel. Furthermore. shoes. if the material is impermeable to water. only contact lenses worn for therapeutic reasons can be permitted in the laboratory. In that event. nitrile. rayon and polyesters are not.” Masks may be used to minimize exposure to noxious fumes. Laboratory Operations & Safety Protocol 34 of 65 V1:2010 . but they are by no means total respiratory protection as air masks. Before each use. belts) can be especially hazardous because many chemicals can be absorbed in the leather and then held close to the skin for long periods. and their suitability for the operation being conducted. It is best not to use gloves made either entirely or partly of asbestos. Such personnel should not wear loose. sharp edged objects. thickness and permeation rate and time) are known. However. Coats and aprons do not significantly resist penetration by organic liquids and. dip into chemicals. which are self-contained breathing apparatuses with their own air tanks. These. or torn clothing and unrestrained long hair. capillary action tends to hold the offending liquid under the contact lens and against the surface of the cornea. Plastic or rubber aprons provide better protection from corrosive or irritating liquids but can complicate injuries in the event of fire. Furthermore. he is warned against absorption through the skin of such substances as HCN gas. skimpy. Leather gloves may be used for handling broken glassware. plastic aprons can accumulate considerable static charge and should be avoided in areas where flammable solvents could be ignited. Proper gloves should be worn whenever the potential for contact with corrosive or toxic materials and materials of unknown toxicity. Finger rings can react with chemicals and also should be avoided around equipment that has moving parts. combustibility) to the wearer. especially any that has become contaminated. Use of Gloves. If the possibility of chemical contamination exists. and synthetic materials such as Nomex or Tyvek are satisfactory. aniline vapor. Double gloving is recommended when handling highly toxic or carcinogenic materials. personal clothing that will be worn home should be covered by protective apparel. for inserting glass tubes into rubber stoppers.except for therapeutic reasons… In the event of a chemical splash into an eye. and very hot or very cold materials. although the wearer is protected against inhalation exposure. Glove materials are eventually permeated by chemicals. Insulated gloves should be used when working at temperature extremes. the removal of the contact lens to achieve immediate irrigation is made nearly impossible by involuntary spasm of the eyelid… Gases and vapors can concentrate under the lenses and cause permanent eye damage.e. and tears. It is the responsibility of the lab supervisor to determine whether specialized hand protection is needed for any operation and to ensure that needed protection is available.g. and may itself present a hazard (e. they can be used safely for limited time periods if specific use and glove characteristics (i. a known carcinogen. For these reasons. Loose or torn clothing and unrestrained long hair can easily catch fire. Clothing and Protective Apparel. the particular hazard involved. The clothing worn by lab workers can be important to their safety. gloves should be inspected for discoloration. should be removed immediately. Respiratory Protection. Laboratory coats and aprons are intended to prevent contact with dirt and the minor chemical splashes or spills encountered in lab-scale work. punctures. Before removal. gloves should be washed. are primarily a protection for clothing. Skimpy clothing offers little protection to the skin in the event of chemical splash. Contact lenses can also trap particulate foreign matter in the eye and thereby produce abrasion of the cornea. These materials differ in resistance to various substances. if significantly contaminated by them. Common glove materials include neoprene. Such items must be removed promptly and decontaminated or discarded. however. or become ensnarled in apparatus and moving machinery. Gloves should be selected on the basis of the material being handled.

Safety shoes are used to protect the feet against injuries from heavy falling objects. or against lacerations from sharp edges. application of cosmetics. would likely result in a foot or toe injury. food. or storage of utensils. Mechanical pipeting aids are to be used for all pipeting procedures. smoking. of objects weighing more than 15 lbs. Personnel should wash their hands immediately after completion of any procedure involving toxic chemicals and when they leave the lab. if dropped. Dry sweeping or dry mopping contaminated laboratory floors could re-aerosolize contamination. sandals. Minimizing Aerosols. 2. Shoes should be worn at all times in laboratories or other areas where chemicals are used or stored. places a physical barrier between the workers in the lab and the chemical reaction. Discharge fluids from pipets as close as possible to the fluid level of the receiving vessel. Access to laboratories which have toxic chemicals should be controlled. sprays. Chemical fume hoods are the primary containment device in the laboratory to control airborne contaminants generated during experimental procedures. but also reduces interruptions to the lab worker which could lead to accidents. Personnel Practices. Safety shoes are required for personnel whose job duties require the lifting. or cloth sneakers should not be worn in labs or areas where mechanical work is being done. drinking. Chemical fume hoods provide personnel protection by means of directional airflow from the laboratory into the hood through the face opening. The use of liquid soap dispensers is recommended. The laboratory door should be kept closed while experiments are in progress. the laboratory worker should develop techniques which will minimize the creation of aerosols: 1. Rapid mixing of liquids with pipets by alternate suction and expulsion or forcibly expelling material from a pipet should be avoided.Foot Protection. especially with the hood sash closed. and minor explosions. Floors should be cleaned with a wet mop or with a vacuum cleaner equipped with a HEPA filter. fires. Since all procedures involving an open vessel of liquids or powders generate aerosols. Care should also be taken when discarding contaminated cloves or plastic backed absorbent paper used to cover the work surface so that contamination is not aerosolized in the process. against crushing by rolling objects. This airflow reduces the potential for escape of airborne contaminants into the laboratory. Decontamination of Work Surfaces. etc. Perforated shoes. Procedures involving volatile chemicals and those involving solids or liquids that may result in the generation of toxic aerosols should be conducted in a chemical fume hood rather than on the open bench.4 Handling Toxic Materials Access Control. These should be placed in properly-labeled waste containers for disposal. Placing a reacting chemical system within a hood. Use of Chemical Fume Hoods. 6. chewing of gum. This not only protects those people who might otherwise enter the lab. 3. Laboratory Operations & Safety Protocol 35 of 65 V1:2010 . or moving. 4. The practice of mouth pipeting should also not be allowed. or allowing the contents to run down the wall of the receiving vessel. Eating. Dropping the contents from a height generates greater aerosol. which. carrying. or food containers should not be allowed in labs where toxic materials are used. Other items and equipment which become contaminated during experimental activity should be decontaminated with the appropriate solvent. Work surfaces should be protected from contamination by using disposable plastic backed absorbent paper or stainless steel trays. This barrier can afford workers protection from chemical splash.

When a sample is removed from the analytical instrument. including water aspirators. Storage vessels containing stock quantities should be labeled: CAUTION – CHEMICAL CARCINOGEN. Hoods should not be used for storage of chemicals. and should include the dates of acquisition and disposition. Quantities should not normally exceed the amounts required for use in one week. it should be placed in a tightly-stoppered sample tube or otherwise safeguarded from contaminating the lab. When using a volatile carcinogen. Work should be carried out as far back in the hood as possible. 5. An inventory of stock quantities should be maintained. 6. 4. are to be placed entirely within a chemical fume hood. Disruptive room air currents should be minimized by avoiding traffic near fume hoods and opening and closing doors near fume hoods while experiments are in progress. when used with chemical carcinogens. is to be protected with an absorbent or liquid trap and a HEPA filter to prevent entry of any chemical carcinogen into the system. Inventory.The following practices should be observed when using fume hoods: 1. 2. The outer container is to be labeled both the name of the chemical carcinogen and the warning: CAUTION – CHEMICAL CARCINOGEN. The primary container must be placed in a durable outer container before being transported. Quantities of chemical carcinogens present in the work area should be kept to a minimum. and their access controlled. Laboratory Transport. Decontamination.5 Handling Carcinogens Procedures for handling toxic materials also applies to chemical carcinogens. Keep sash as low as possible. Check efficiency of the hood by measuring its average face velocity regularly. Storage vessels containing working quantities should be labeled: CAUTION – CHEMICAL CARCINOGEN. all of the materials needed for a procedure are present in the hood to eliminate disruption of airflow by carrying equipment in and out during a procedure. Storage. The procedures outlined in this section are additional precautions in dealing with carcinogenic materials. Stock quantities of chemical carcinogens are to be stored in designated storage areas. Chemical carcinogens Laboratory Operations & Safety Protocol 36 of 65 V1:2010 . as much as possible. Contaminated materials which are transferred from work areas to disposal areas must first be placed in a closed plastic bag or other suitable impermeable and sealed primary container. Storage vessels containing chemical carcinogens are to be first placed in an unbreakable outer container before being transported to lab work areas. Experiments should be planned so that. Each vacuum service. 3. 6. 7. and Identification. Hood work areas should be clear of unnecessary equipment and materials which can disrupt airflow and block vents. Contaminated materials must either be decontaminated by procedures that decompose the chemical carcinogen. Verify airflow PRIOR to doing an experiment by attaching a Kimwipe or ribbon to the sash. Face velocities between 100 to 120 feet per minute are acceptable for most uses. The storage areas should be posted with signs bearing the legend: CAUTION – CHEMICAL CARCINOGEN Authorized Persons Only. Use of Analytical Instrumentation. Working Quantities. vapors or aerosols produced by these instruments should be captured through local exhaust ventilation at the site of their production. Use equipment with legs. Analytical equipment that becomes contaminated should not be used until it has been completely decontaminated. Analytical instruments. When this is impossible. 8. Protection of Vacuum Lines. a separate vacuum pump or other device placed in an appropriate chemical fume hood should be used. or be removed for subsequent disposal.

The vapors of flammable liquids are heavier than air and capable of traveling considerable distances. permanganates. and burning rates) of combustible materials likely to be encountered under various conditions of use. If the path of vapor with the flammable range is continuous. Materials susceptible to spontaneous combustion include oily rags. heating mantles. finely divided pyrophoric metals. When flammable materials are used in the lab. Flashpoint: the minimum temperature at which a liquid gives off a vapor in sufficient concentration to ignite when tested according to ASTM D 56-79. Preferred head sources include steam baths. the flame will propagate itself from the point of ignition back to its source. This is of special concern when working in a dry environment such as an air-conditioned room or a room with a dehumidifier. dust accumulations. chlorates. A means for assuring adequacy of clean-up should be provided. ignition requirements. static electricity. the bonding can be made to the liquid rather than to the container. peroxides. oil baths. 3. This possibility should be recognized. 1. and of the appropriate procedures top use in handling such substances. The basic precautions for safe handling of flammable materials include the following: 1. and special note should be taken of ignition sources lower than that at which the substance is being used. Spontaneous Ignition or Combustion: this takes place when a substance reaches its ignition temperature without the application of external heat. Many common laboratory solvents and chemicals have flashpoints that are lower than room temperature. The possibility of spontaneous combustion should be considered. static-generated sparks should be avoided by bonding and use of ground straps. 6. For example: A steam line or a glowing light bulb may ignite carbon disulfide (ignition temperature 80 oC).6 Flammable Materials The ability to vaporize. Flammable vapors from massive sources such as spillages have been known to descend into stairwells and ignite on a lower story. A flammable liquid does not itself burn. Prevention of fires and explosions requires knowledge of the flammability characteristics (limits of flammability. When transferring flammable liquids in metal equipment. lighted matches. and hot air baths. organic materials mixed with strong oxidizing agents (such as nitric acid. it is the vapors from the liquid that burn. When nonmetallic containers (plastic) are used. 2. The rate at which different liquids produce flammable vapors depends on their vapor pressure. Flammable substances should never be heated by using an open flame. Ignition Temperature (Auto-ignition temperature): the minimum temperature required to initiate or cause self-sustained combustion independent of the heat source. and hot surfaces. for instance wipe tests or fluorescence tests. close attention should be given to all potential sources of ignition in the vicinity. There are a number of potential sources of spark. or heat in labs which can ignite flammable substances: open flames. Laboratory Operations & Safety Protocol 37 of 65 V1:2010 . and ASTM D 3278-78. flame. 3. Handling.which have spilled out of a primary container so as to constitute a hazard must be inactivated in situ or should be absorbed by appropriate means for subsequent disposal. The degree of the fire hazard also depends on the ability to form combustible or explosive mixtures with air. and phosphorous. alkali metals such as sodium and potassium. ignite. 2. Properties of Flammable Substances. Metal lines and vessels discharging flammable substances should be properly bonded and grounded to discharge static electricity. ASTM D 93-79. and persulfates). Diethyl ether (ignition temperature 160 oC) can be ignited by the surface of a hot plate. water baths. especially when materials are stored or disposed of. and burn or to explode flammable materials varies with the specific type of substance. Flammable substance should be handled only in areas free of ignition sources. Sources of Ignition.

Keep flammable liquids away from heat and direct sunlight. Fire extinguishers are labeled according to their fire fighting proficiency and safety in extinguishing various types of fires. and.7 Handling Explosive/Reactive Materials The large number of different classes of chemicals potentially present in a research laboratory poses increased risks from accidental hazardous chemical reactions or explosions. offer first defense against flammable liquids or electrical fires without leaving a powder residue which could harm electronic equipment. Hazardous reactions may cause any one or more of the following: heat generation. For easy identification. 4. Fire Extinguishers. formation of shock or friction sensitive compounds. Flammable liquids must not block lab aisles or exits. Flammable Liquids: Fires in solvents and other flammables liquids require dry chemical. 6. light.4. 5. These are identified accordingly: 1. wood. Electrical Equipment: Fires in wiring. labels A. Using the “wrong” type of extinguisher on a fire can endanger the user and make the fire worse. solubilization of toxic substances. formation of substances of greater toxicity. 4. Flammable liquids must be stored so that accidental contact with strong oxidizing agents (such as permanganates or chlorates) is avoided. Halon or CO2 extinguisher labeled B. 5. energized electrical equipment and other electrical sources require a dry chemical. formation of flammable gases. When a flammable liquid is withdrawn from a drum. 2. or when a drum is filled. fuse boxes. more recently. 2. Flammables stored in the open in the laboratory work area shall be kept to the minimum necessary for the work being done. dispersal of toxic dusts/mists/particles. Most chemical laboratory fire hazards require dry chemical multipurpose extinguishers (ABC) which must be installed in hallways. allowed to stand in open containers. Containers of flammable liquids shall not be drawn from or filled within buildings unless provision is made to prevent the accumulation of flammable vapors in hazardous concentrations. 6. C. or handled in any other way. An exhaust hood should be used whenever appreciable quantities of flammable substances are transferred from one container to another. heated in open containers. rubber. Ventilation is one of the most effective ways to prevent the formation of flammable mixtures. fire. volatilization of toxic or flammable substances. containing Halon 1211 or CO2. or D. They may result when incompatible chemicals are accidentally spilled. and violent polymerization. “Gas” extinguishers. and many plastics require water or dry chemical type extinguisher labeled A. Metals: Combustible metals such as magnesium and sodium require special extinguishers labeled D. when they are inadvertently mixed as chemical waste. 3. Use refrigerators that have electrical contacts (door switch. or when they are unwittingly combined during experimental procedures. or CO2 extinguisher labeled C. A hazardous reaction occurs when two or more incompatible chemicals result in an undesirable or uncontrolled reaction with adverse consequences. cloth. Laboratory Operations & Safety Protocol 38 of 65 V1:2010 . Ordinary Combustibles: Fires in paper. Storage Rules 1. explosion. pictograms are used to indicate the type of fire on which an extinguisher is to be used. both the drum and other equipment must be individually electrically grounded and bonded to each other. B. Flammable liquids must not be stored in domestic type refrigerators. 3. Halon. pressurization in closed vessels. thermostat) removed or exteriorized. formation of toxic vapors.

It is prudent to check for incompatibility wherever a change is made in chemical procedures. the disaster is compounded by the adverse reaction. Incompatibility of chemicals is the prime reason for not storing chemicals on the shelf alphabetically. If there is an accident. Laboratory Operations & Safety Protocol 39 of 65 V1:2010 .It is easy to fall into the trap of becoming complacent with certain chemicals used everyday with certain procedures.

Ignitability.5. Only trace quantities of oils associated with cleaning and washing operations should be released to the sanitary sewer. there must be careful consideration about which wastes can be combined. Acids and Bases. Aqueous solutions with a pH less than or equal to 2. 3. 4.35 mm per year at 55 oC.Chapter 7 Waste Disposal 7. Examples of halogenated solvents include methylene chloride. but followed by flushing with copious volumes of water. Normally unstable and undergoes violent changes. Corrosivity. detoxify. Liquids with a flashpoint less than 60 oC. mutagenic. These should be converted to sulfide. and carbon tetrachloride. which is less soluble.g. A waste stream generated from a laboratory procedure should not be combined with other chemical wastes. or dermal LD50 of 200 mg/kg (rabbit). diluted to non-flammable concentrations. Small volumes of acids and bases can be diluted and discharged in the sanitary sewer. Reactivity. capable of detonation or explosive reaction. Different waste materials should be segregated whenever possible. 2. sodium metal. or suspected carcinogenic. Acids and bases containing heavy metals must not be disposed to the sewer system. carcinogenic. Laboratory Operations & Safety Protocol 40 of 65 V1:2010 . liquids which corrode steel at a rate greater than 6. Toxicity. Fatal to humans in low doses. Efforts should be made to decontaminate. inhalation LC50 of 2 mg/L (rats). If this is not practical. reacts violently with water.2 Waste Reduction Minimizing chemical waste generation also minimizes safety hazards. forms potentially explosive mixtures with water of pH conditions between 2 and 12. 7. Recyclable materials should be kept separate from other waste. The fewer the number of chemicals associated with a waste. Halogenated solvents must not be combined with flammable nonhalogenated solvents. or otherwise render the waste nonhazardous. teratogenic. or greater than or equal to 12. e. toxic. Heavy Metals. the more economical is the disposal method for that waste. chloroform. It is encouraged that laboratories consider ways of reducing the volume of waste or preserving the reuse of the materials through the redesign of experiments. Non-halogenated flammable solvents may be sent to the incinerator and must be free of heavy metals and reactive materials. neutralize. before final disposal.1 Waste Characteristics Hazardous wastes are classified according the following: 1. absorption of moisture. less than oral LD50 of 50 mg/kg (rats). solids capable of burning vigorously and persistently after ignition through friction. Disposal of solvents to the sanitary sewer is limited to low-toxicity solvents. miscible in water.5.3 Handling Specific Wastes Individual Waste Streams. or spontaneous chemical changes at standard temperature and pressure. Halogenated Solvents. 7. Oils. Non-Halogenated Flammable Solvents. oxidizers.

Substances or conditions which could result in an explosion. and the approximate percentage of each compound. to caution the handler. Corrosive materials with pH less than 5. Each compound in the waste containers must be listed by its complete chemical name. pasteur pipettes. Obtain waste bottles for chemical wastes from the stockroom 2. 2. A bottle with a barcoded label will be issued by the stockroom personnel to the student. 7. and solid waste in pounds or kilograms.Sharps. 3. Gallon-sized four-liter glass reagent bottles are generally the most convenient. Materials that may create a fire or explosive hazard. Filled-up waste bottles should be submitted to the stockroom for signing of clearance at the end of the school year Safety Carriers. Provide the following information: • Name • Chemical waste classification • Chemical wastes to be kept in the bottle • Name of mentor 3. The terms most appropriately describing the waste should be included. 7. or flammable gas generation should be explained. giving the container size and indicating whether it is full. Waste Characteristics. Do not use abbreviations. including but not limited to syringes. Sharps. If the waste is an acid or a base. 3/4 full. Glass collection containers must preferably be stored in rubber safety carriers to protect against breakage and spillage. Laboratory Operations & Safety Protocol 41 of 65 V1:2010 . Liquid waste is to be given in gallons or liters. 7. The amount of waste. fire. All hazardous characteristics of waste should be identified. Procedure for obtaining chemical waste bottles 1. Any toxic or reactive hazards. the approximate pH of the waste should be indicated beside “Corrosive” on the label. Amount. capillary tubes and scalpels should be placed in puncture-resistant waste collection containers prior to disposal. Carcinogenicity. 4. Special Handling Considerations.5 Waste Containers Containers.6 Disposal to Sewerage System Avoid discharges to the sanitary sewer of the following: 1. If metal or plastic containers are used. 4. razor blades. or teratogenicity should be specified beside “Toxic”. 2. should be noted. mutegenicity. be sure that the container material is compatible with the waste. Principal Constituents.4 Labeling Each container must be labeled with the following information: 1. etc. heat generation.

3. Na. NH4 • Oxides: Mg. Si. or contamination of sludge of effluent from the wastewater treatment. Ca. However. 5. 4. Sr. the following nonhazardous materials may be placed in ordinary trash bins for disposal: Agarose Alumina Aluminum oxide Ammonium phosphate Calcium carbonate Calcium oxide Calcium phosphate Calcium sulfate Citric acid Dextrin Glycine Magnesium carbonate Magnesium chloride Magnesium sulfate Potassium carbonate Potassium chloride Potassium sulfate Sephadex Silica gel Sodium chloride Sodium citrate Sodium phosphate Sodium sulfate Stannous oxide Starch Sugars Titanium oxide Materials listed below in quantities up to about 100g or 100 mL at a time are suitable for disposal down the drain while flushing with excess water Organic Chemicals • Alkanols with fewer than 4 carbons • Aliphatic aldehydes with fewer than 5 carbons • Amides with fewer than 5 carbons • Aliphatic amines with fewer than 7 carbons • Carboxylic acids with fewer than 6 carbons • Esters with fewer than 5 carbons • Proteins. K. Ca Laboratory Operations & Safety Protocol 42 of 65 V1:2010 . Mg. the containers should be labeled and marked as “Non-hazardous. Mg. Sr. Ca. 7. Sr. Al. Solid or viscous materials in amounts to obstruct flow or interfere with operations.” 2. K. K. Non-hazardous wastes may be disposed of as ordinary trash. nucleic acids. Non-hazardous wastes in liquid form may be disposed of down the drain. Discharges of any toxic material in volume of strength to cause interference with waste treatment processes. Co. soluble fats and their precursors Inorganic Chemicals • Sulfates: Na. NH4 • Carbonates: Na. K. Mn.7 Disposal of Chemical Wastes Non-hazardous Chemical Wastes 1. Cu • Chlorides: Ca. carbohydrates. Mg. Heat discharges which will inhibit biological activities or increase the waste water treatment effluent about 40 oC. Fe. Mg. In general. Ti. NH4 • Borates: Na. Ca.

Laboratory Operations & Safety Protocol 43 of 65 V1:2010 . These wastes will be hauled by DENR-accredited transporters for treatment and disposal.Hazardous chemical wastes Hazardous chemical wastes should be placed and segregated into leak-proof barcoded waste bottles following the classification of the DENR-EMB (See Table 5 in the Appendix).

Fire extinguisher/blanket Laboratory Operations & Safety Protocol 44 of 65 V1:2010 . Food is stored outside the work area in cabinets or refrigerators designated specifically for that purpose. 4. There are designated refrigerators (Biochemistry and Room 103 NCIC labs) for short-term storage (2 months or less) and a cryogenic container (Rm 103 NCIC lab) for long-term stock storage. fungi. (Refer to Table 6 of the Appendix) 3. Only organisms under BSL 1 are allowed in the laboratory. For bacteria specimens. 8.2 Storage 1. virus. the "primary" container should be carried within an outer "secondary" container. there are four levels as specified by US Department of Health and Human Services and National Institutes of Health. When leaving the lab.4 Handling 1. 2. Store the samples in these designated places only to avoid spreading and contamination of bio-free chemicals and solutions. 8. From time to time. face shield or other splatter guard) for anticipated splashes or sprays of infectious materials to the face when agents must be handled outside the BSC (biosafety cabinet). 5. Samples must be properly labeled to immediately identify the organism present. University of Wyoming. mask. The secondary container should be sealable and non breakable. 6. Microorganisms refer to organisms of microscopic or sub-microscopic sizes such as bacteria. The lab apron or coat must be laundered every two weeks. Do not wear contaminated gloves outside the lab. remove and leave lab apron or coat in the lab. Persons wearing contact lenses should also wear eye protection. 3. spray a solution of 70% alcohol (usually ethanol) on the gloves to disinfect. only mechanical pipetting devices are permitted.Launceston Campus 1. and Microbiology Safety and Staff Induction Manual. Always wash hands after removing gloves and before leaving the lab. University of Tasmania . Wear appropriate face protection (goggles. Always wear mask and put on gloves in handling samples. 7. a.1 General Information Most of the procedures are adapted from Environmental Health and Safety.Chapter 8 Biological Samples: Handling and Waste Disposal 8. Lab apron must be worn at all times. Princeton University. Remove and change gloves when overtly contaminated or when torn or punctured. Before using any of these samples. 8. Do not wash or reuse disposable gloves. Do not pipette by mouth.3 Transport When transporting samples to or from the laboratory. First aid kit b. know the biosafety level (BSL) of the organism first. 2. and yeast. Familiarize yourself with the location and operation of the following emergency items for each laboratory. 2.

8. 8. To prevent accidents. make sure that the door of the isolation room is closed to minimize the disruption of the airflow. time and contents of the cycle. mask. No slides or cultures are to be taken from. Leave for 10 minutes and then mop up. 6. For minor spills. or brought into the laboratory without permission of the instructor or laboratory in-charge. working with materials from the clean to the dirty side. The standard autoclave cycle used in this laboratory is 15 psi for 15 minutes.c. It is housed in the isolation room in C305. 2. if the cabinet is not left running. 4. The working area should be wiped with disinfectant at the beginning and end of the laboratory session. put on gloves. 5. 4. Always use heat safety gloves when removing items from the pressure cooker. 11. call the instructor immediately. One pressure cooker is designated for sterilization purposes while another is solely used for decontamination. and gloves. 9. This applies particularly to the flaming of the bacteriological loop: the loop should be drawn gradually from the cooler to the hotter parts of the flame. splashes and the production of aerosols. 3. Wipe the bottom and side of the hood surfaces with disinfectant when work is completed. 12. Carry out procedures so as to minimize the risks of spills. At a minimum. Always wash your hands before leaving the laboratory. 11. Before using the equipment. Turn the cabinet on for at least 10 . A heat sensitive indicator (autoclave tape) should be used in every load. wait until the pressure drops to ~5 psi before opening the exhaust valve.5 Laboratory Equipment Autoclave 1. Delay manipulation of materials for approximately one minute after placing the hands/arms inside the cabinet. Consider the materials necessary for the planned work in the cabinet. Working in a BSC (adapted from Environmental Health and Safety. 5. Gas isolation switch d. Place items into the cabinet so that they can be worked with efficiently without unnecessary disruption of the air flow. cover spill with paper towel and pour on disinfectant. 6. A laminar flow cabinet is also available in the Biochemistry lab. If you have an accident of any kind. Disinfect work surface with 70% alcohol or other suitable disinfectant.Eyewash station e. this will include a laboratory apron/coat. Laboratory Operations & Safety Protocol 45 of 65 V1:2010 . Princeton University) 1. 3. Always wear safety glasses when opening the pressure cooker. The BSC in the laboratory is a Class 2 cabinet designed to provide personnel. environmental. The lid should only be open when the pressure is zero. Adjust the working height of the stool so that the worker's face is above the front opening. 10. Laminar Flow Cabinet/Biosafety Cabinet (BSC) 1. Do not disturb the airflow by covering any of the grillwork with materials. and sample/product protection. Minimize the frequency of moving hands in and out of the cabinet. 10. 9. 2. indicate the date.15 minutes prior to use. Wear appropriate personal protective equipment. 2. Exits 8. 7. Work at a moderate pace to prevent the air flow disruption that occurs with rapid movements. In the logbooks of either pressure cooker.

2. For example. 3. preferably made of glass or other autoclave-compatible materials. The materials must be decontaminated immediately. After decontamination. others) must be prepared. (adapted from Environmental Health and Safety. how pathogenic are the organisms contained or likely to be contained in the spill. A decontamination procedure can be as simple as clean-up with a solution of detergent. 8. The container must be covered by aluminum foil.7 Clean-up Procedure 1. or Wipe with 70% alcohol 1. tongs. within the day or the day after. consummable items. In the event of spill incidents. culture loops. Liquid Decontamination • • • Add liquid chlorine bleach to provide a final 1:10 dilution Let stand at least 20 minutes Discard down the drain Surface Decontamination • • Wipe with 1:10 dilution of chlorine bleach. bleach. put the glassware and other materials directly touched by culture media or contaminated with microorganism in a box or leave them inside the BSC. the following steps must be considered. After the microbial laboratory activity. Handle broken glassware with brush and dustpan. has the spill resulted from a container knocked over on a bench with low potential for the generation of aerosols. 2. and other materials used in the microbial activity must be decontaminated. For example. recap. Place full containers in an autoclave bag and sterilize before disposal in waste boxes. or smashed in some way with a greater potential for the generation of aerosols? Laboratory Operations & Safety Protocol 46 of 65 V1:2010 . or remove used needles from disposable syringes or otherwise manipulate such units by hand before disposal.6 Waste Disposal 1. A container for used items (pipet tips. All glassware. pipet. 4. Clean the working area using surface decontamination procedure. break. 2. shear.8. or has a container been dropped. Dispose of needles and syringes in the puncture resistant container provided in the laboratory for this purpose. Princeton University) Assessing a Biohazard Spill The biological nature of the spill. if possible. The standard decontamination cycle used in this laboratory is 15 psi for 15 minutes. Do not bend. or forceps – not directly with hands. and is infection likely to be acquired by the respiratory route? The physical nature of the spill. 3. Sterilization is done using the pressure cooker designated for decontamination purposes. and water or as thorough as sterilization. liquid wastes are thrown down the drain while solid wastes are thrown in trash bins.

4. Remove and replace any contaminated protective clothing. pneumoniae that has been dropped onto the floor would be considered a "major biohazard spill". gloves. 4. warn others of spill and all must leave the room immediately. Major biohazard spills Generally considered to be spills of major risk with larger volume and considerable production of splashes and aerosols. vomit. urine. Treat all body fluids as if they are infectious. face masks and safety glasses" before entering the spill area. vaginal secretions. coli that has been knocked over on the bench would be considered a "minor biohazard spill" whereas a 500 ml broth containing S. 3. 1. put an absorbent material over the fluid. In the event of spillage. Lay paper towels wetted with disinfectant onto the spill and leave for 30 minutes. 3. feces. 3. Close doors and place a "DO NOT ENTER" sign on the door. 8. 2. first wash hands with strong detergent followed by generous spray of 70% alcohol. 6. Use disinfectant to wipe over areas around the spill that are likely to have been contaminated with splashes and aerosols. Notify laboratory in-charge(preferably) or other faculty member The clean up team should all don "gowns. Remove gloves and wash hands. 9. Wipe over general area again with paper towel dampened with disinfectant. For example. Do not pour disinfectant directly onto the spill as this may create additional aerosols. 1. Determine the extent of the spill: pour disinfectant around the edge of the spill and allow it to run "into the spill". 5. 10 ml broth culture of E. 8. 1. After 10 minutes. Lay down absorbent material wetted with disinfectant over the spill and allow to sit for 10 minutes. Discontinue working in the immediate area. Remove any contaminated clothing and wash any contaminated body surfaces. 2. Do not re-enter the room until a minimum of 30 minutes has elapsed. 5. and oral or nasal secretions. semen. Always wear gloves in dealing with body fluids. Put on gloves. 2. If hands have been contaminated. mop up spill and place contaminated materials into autoclave bag. 5. Dispose the gloves by sealing them in a plastic bag and placing it in a trash bin. 7. 4. 6.The volume of liquid spilt. 8.8 Body Fluids Body fluids include blood. Minor biohazard spills Generally considered as a spill of minimally hazardous material with low potential for generation of aerosols. 7. Clean and disinfect the area with freshly prepared 1:10 (bleach: hot water) solution. Hold breath. Laboratory Operations & Safety Protocol 47 of 65 V1:2010 . Wash the hands thoroughly with hot water and soap. Don’t forget to wear gloves. Dispose the absorbent material by sealing them in a plastic bag and placing it in a trash bin.

A. and submit to the secretary of the Chemistry Department. and for yourself). and locked by the security guard at 10:00 p. and submit to the instrument manager of the Chemistry Department. Photocopy duplicates (for the Department and for yourself).m. while overnight forms must be handed in to the Department secretary at least 2 days before the event. It is also the supervisor’s responsibility to file an injury/accident report with the Chemistry Department. At least two people should stay for overnight work. As a rule.m. Try to work out a schedule that will go from 6:00 a. Overtime forms may be processed within the day of the scheduled overtime. Photocopy duplicates (for the Department and for yourself). or medical treatment in excess of first aid will be investigated by the Chemistry Department to determine liability or the possible need for corrective action.1 in following pages serves as the Overtime/Overnight Request form. and submit to the secretary of the Chemistry Department.m. This will allow the office to inform the University administration and security. Any injury resulting in lost work time. The FORM A.m. overnight stays are discouraged. fill-in. The FORM A. It is the student’s/researcher’s responsibility to notify his supervisor immediately of any work-related injury or illness. Photocopy triplicates (for the Department. If the injured person is not able to do so. The FORM A. Work that will go beyond 10 p. hospitalization. It is the supervisor’s (faculty’s) responsibility to ensure that the student receives prompt treatment of the injury by obtaining first aid or assistance to medical treatment. a co-worker should notify the supervisor as soon as possible. A. work that will go beyond 12 midnight is considered overnight. the Physical Plant. File injury/accident reports with the secretary of the Chemistry Department. fill-in. If an experiment requires longer than 15 hours. you may work until 10 p.1 Form for Overtime or Overnight Lab Work Schmitt Hall is opened to the public by the building manager by 6:00 a.2 Form for Reporting Lab Accidents All personal injuries and accidents should be reported in order that corrective action may be taken to minimize the probability of recurrences.2 in following pages serves as the Accident Report form. Laboratory Operations & Safety Protocol 48 of 65 V1:2010 . to 10:00 p. overnight stay shall be considered. These require you to fill in the form below and seek final approval from the Department Chair. as long as there are still faculty in the building to let you out of the building. fill-in..3 serves as the Instrument Authorization form. is considered overtime.3 Instrument Authorization Form Students who wish to use an instrument in the department are required to be checked-in first by the faculty-in-charge.Appendices A.m.m. If your supervisor allows it.

and Methods Reason: State why this work cannot be done during regular building hours.FORM A. After filling-in the above items. Chemicals. get the following signatures: (print name and sign) Your Signature Your Supervisor Building Manager Chair Note: Trplicate! Laboratory Operations & Safety Protocol 49 of 65 V1:2010 .1 : Overtime/Overnight Request Full Name (please print) Date of Request Nature of Request (encircle one) Overtime Overnight Time of Event Date of Event Name of Accompanying Person(s) Signature(s) List of Special Equipment.

FORM A.2 : Accident Report
Full Name (please print) Date of Report

Lab Class and Section

Time of Accident

Date of Accident

Name of Injured Person(s)


List of Equipment & Chemicals Involved

Narrate the accident chronologically and in detail. Supply all the pertinent facts.

What first aid or medical attention were applied?

After filling-in the above items, get the following signatures: (print name and sign) Your Signature Witness 1 Witness 2 Chair

Note: Duplicate!

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FORM A.3 : Instrument Authorization Form
Ateneo de Manila University School of Science and Engineering Department of Chemistry
INSTRUMENT AUTHORIZATION FORM Student:____________________________________ Instrument:_____________________ Name of Mentor:_____________________________ Mentor’s Approval:______________ Purpose of Instrument Use: ___________________________________________________ __________________________________________________________________________ Waiver: I have read and I fully understand the “Policies on the Use of the Department’s Facilities and Equipment.” I _______ agree to follow the guidelines and procedures _______ do not agree to follow the guidelines and procedures because:______________

INSTRUMENT CHECK-IN Date of Check in: ________________________ Faculty in-charge: ________________________ ____Instrument General Working Principles ____Instrument Parts ____ Safety and Precautionary Measures ____ Short Notes of the Procedure ____ Important Reminders and Considerations Remarks:____________________________________________________________________---------------------------------------------------------------------------------------------------------------AUTHORIZATION CERTIFICATE Date: __________________________________ Faculty in-charge: ___________________________ I hereby certify that Mr./Ms. _____________________________ is authorized to use the ____________________________________. Limitations of use:_______________________________________________________________ -------------------------------------------------------------------------------------------------------------------------INSTRUMENT CHECK-OUT Date of Check out:____________________ Faculty in-charge: _________________________ ____Instrument Parts ____Instrument Accessories ____Consumables ____Working environment

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Figure 1: Lab benches for General Chemistry Lab. Each bench has its own locker, equipment set, hood, sink, power and gas lines, and broom for sweeping up broken glass.


2: Fume hoods for (A) the Analytical Lab, and (B) General Chemistry Lab. The fume hoods are located either on the sides of the laboratory rooms (A), or individually, for each lab bench (B).

Figure 3: Power, gas,
and water lines. These are color coded as follows: RED for power lines, GREEN/BLUE for water lines, and YELLOW for gas lines. The valves are also similarly color coded.


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as shown here in the Organic Lab (A) and General Chemistry Lab (B). B Figure 6: Aspirator set-up for the clean-up of mercury spills. Laboratory Operations & Safety Protocol 53 of 65 V1:2010 . A Figure 5: with exits also in all Floor plans directions to fire (red arrows) are placed prominently rooms.Figure 4: Fire extinguishers (A) and showers (B) are placed prominently in all labs.

Figure 8: GHS Pictograms and Hazard Classes Laboratory Operations & Safety Protocol 54 of 65 V1:2010 . There are several throughout the building.Figure 7: The new (March 2004) fire alarm system in Schmitt Hall: Breaking the cover and pushing the fire alarm button triggers the alarm. Occupants are requested to be familiar with the location of the push button nearest their office/lab.

Figure 10: Logical computer hardware setup Laboratory Operations & Safety Protocol 55 of 65 V1:2010 . Note the dolly on the right for proper transportation of gas cylinders.Figure 9: Compressed gas tanks tethered properly and capped.

Figure 11: Overview of CMIS Requesting Procedure Laboratory Operations & Safety Protocol 56 of 65 V1:2010 .

junior faculty 3-Department secretary. lab managers. senior faculty 4-Administrators. CMIS User-levels CMIS Feature Access to chemical information Access to storage locations Access to stock levels Setting up and modification of data Requesting services Chemical request management Password management Reporting services Administrative functions Access to Hazardous Waste Inventory System (HWIS) 1 x User-level 2 3 x x x x x x x x x x x x 4 x x x x x x x x x x x 1-Guest: for undergrad and grad students. formulation and application of standards for the transport and disposal of effluent. 2-TAs. rules and regulations which govern various aspects related to chemicals. etc. RAs. Regulation on the use. technicians. nitric acid. storage and disposal of toxic chemicals and hazardous and nuclear wastes Air emissions. EMB Implementing Agency Philippine National Police Coverage Restriction on purchase of nitrates.Tables Table 1. Environmental Management Bureau (EMB) Chemical waste management of toxic and hazardous wastes (See Appendix). technician-in-charge of CMIS Table 2. Rule or Regulation RA 8294: Regulation on explosives and materials used in the manufacture of explosives (1997) RA 6969: Toxic Substances and Hazardous and Nuclear Wastes Control Act (1990) DAO 92-29: Implementing Rules and Regulations of RA 6969 RA 8749: Philippine Clean Air Act (1999) DAO 00-81: Implementing Rules and Regulations of RA 8749 RA 9275: Clean Water Act (2004) DAO 05-10: Implementing Rules and Regulations of RA 9275 EMB Water quality management in all water bodies. development of guidelines for re-use of wastewater etc. ban on incineration Laboratory Operations & Safety Protocol 57 of 65 V1:2010 . movement. List of laws. sewage and septage offsite. Abatement and control of pollution water bodies. Law.

sale. PCB articles and PCB packaging in commercial buildings and facilities. distribution. Treatment. Importation. This also includes the use and possession by electric utilities and suppliers and the generation. This includes proper segregation. manufacture. treatment and disposal of PCB wastes. Sulfuric acid 9. List of PDEA-controlled chemicals 1* 1. PCB equipment. administration. Importation. Acetic anhydride 2. 1-Phenyl-2-propanone 11. storage and disposal of asbestos-containing materials or asbestos containing wastes. PCB-contaminated equipment. Potassium Permanganate 2* 1. Piperonal 12. formulation of guidelines and targets for solid waste avoidance and volume reduction and setting up of controlled dumpsites. Ephedrine 4.4-Methylenedioxyphenyl-2-propanone 9. Hydrochloric acid 5. non-PCB equipment. Implementing Rules and Regulations for specific substances Law. storage and disposal of cyanide-bearing or cyanide contaminated wastes. Department of Health (DOH) Table 3. delivery. sanitary landfills. Ergotamine 6. Methyl ethyl ketone 6. Lysergic acid 8. N-Acetylanthranilic acid 3. Rule or Regulation DAO 97-39: Chemical Control Order for Cyanide and its Compounds Implementing Agency EMB Coverage Importation. DAO 00-02: Chemical Control Order for Asbestos EMB DAO 04-01: Chemical Control Order for Polychlorinated Biphenyls (PCBs) EMB Table 4. Ergometrine 5. storage. Phenylacetic acid 7. Anthranilic acid 3. sale. Piperidine 8. transportation. and other waste depots. 3. Treatment. storage. Norephedrine 10. manufacture of asbestos. collection. Ethyl ether 4. Regulation of the importation. Acetone 2. transport. Toluene Laboratory Operations & Safety Protocol 58 of 65 V1:2010 .RA 9003: Ecological Solid Waste Management Act (2000) DAO 01-34: Implementing Rules and Regulations of RA 9003 EMB Management of solid wastes. use and distribution of cyanide and cyanide compounds. transport. transfer. Isosafrole 7. manufacture. possession or use of prohibited and regulated drugs and precursor chemicals RA 6425: Dangerous Drugs Act (1972) Philippine Drug Enforcement Agency (PDEA). distribution and use of PCBs. treatment and disposal of solid waste.

0 mg/L based on analysis of an extract Includes all wastes with a total As concentration > 5.0 Acid wastes other than B201 to B208 with pH= 2.0 Caustic soda with pH = 12. DENR Classification of Hazardous Wastes Class A: Wastes with cyanide Wastes with cyanide Description Waste Number A101 Waste containing cyanide with a concentration >200 ppm in liquid waste B: Acid wastes Sulfuric acid Hydrochloric acid Nitric acid Phosphoric Acid Hydrofluoric acid Mixture of sulfuric and hydrochloric acid Other inorganic acid Organic acid Other acid wastes C.5 Potash with pH = 12.0 Organic acid with pH= 2. Alkali wastes Caustic soda Potash Alkaline cleaners Ammonium hydroxide Lime Slurries Other alkali wastes D. Safrole *The salts of the substances in the list whenever the existence of such salts is possible (The salts of hydrochloric acid and sulfuric acid are specifically excluded).5 Lime slurries with pH = 12.5 Alkaline cleaners with pH = 12.0 mg/L based on analysis of an extract Includes all wastes with a total Pb concentration > 5.0 Nitirc acid with pH = 2.0 mg/L based on analysis of an extract B201 B202 B203 B204 B205 B206 B207 B208 B299 C301 C302 C303 C304 C305 C399 D401 D402 D403 D404 D405 D406 Laboratory Operations & Safety Protocol 59 of 65 V1:2010 .0 mg/L based on analysis of an extract Includes all wastes with a total Ba concentration > 100.0 Hydrofluoric acid with pH = 2.0 mg/L based on analysis of an extract Includes all wastes with a total Cd concentration > 5.5 Ammonium hydroxide with pH = 12. Table 5. Pseudophedrine 14.0 Other inorganic acid with pH = 2.0 Mixture of sulfuric and hydrochloric acid acid with pH = 2.5 Alkali wastes other than C301 to C306 with pH = 12.5 Includes all wastes with a total Se concentration > 1. Wastes with inorganic chemicals Selenium and its compounds Arsenic and its compounds Barium and its compounds Cadmium and its compounds Chromium compounds Lead compounds Sulfuric acid with pH = 2.13.0 mg/L based on analysis of an extract Includes all wastes with a total Cr concentration > 5.0 Hydrochloric acid with pH = 2.0 Phosphoric acid with pH = 2.

continuation (Table 5) Mercury and mercury compounds Other wastes with inorganic chemicals Includes all wastes with a total Se concentration > 0. 4. Reactive chemical wastes Oxidizing agents E501 Reducing agents E502 Laboratory Operations & Safety Protocol 60 of 65 V1:2010 . vapors and fumes in a quantity that poses a danger to human health Includes all wastes that are known to contain oxidizing or reducing agents in concentration that cause the waste to exhibit any of the following properties: 1. zinc compounds. It is normally unstable and readily undergoes violent change without detonating. When mized with eater. which when exposed to pH conditions between 2 and 12. 4. inorganic fluorine compounds excluding calcium fluoride Includes all wastes that are known to contain oxidizing or reducing agents in concentration that cause the waste to exhibit any of the following properties: 1. It reacts violently with water. beryllium and its compounds. thallium and its compounds. It is normally unstable and readily undergoes violent change without detonating. 3.5 can generate toxic gases. metal carbonyls. It forms potentially explosive mixtures with water. It is a cyanide (CN) or sulfide (S) bearing wastes. It forms potentially explosive mixtures with water. tellurium and its compounds. 2. which when exposed to pH conditions between 2 and 12. it generates toxic gases. It reacts violently with water. vapor or fumes in a quantity sufficient to present a danger to human health. When mized with eater. 2. vapor or fumes in a quantity sufficient to present a danger to human health. 3. Refer to CCO Wastes containing the following chemicals: antimony and its compounds. it generates toxic gases.5 can generate toxic gases. copper compounds. vapors and fumes in a quantity that poses a danger to human health D407 D499 E.2 mg/L based on analysis of an extract. It is a cyanide (CN) or sulfide (S) bearing wastes.

methyl isobutyl ketone. nitrobenzene.isobutanol. Other mixed Includes all aqueous-based wastes that also meet one or more of the subcategories.hexanol. Carbon disulfide. acetone. cresylic acid. benzene. it also includes all still bottoms from recovery of these solvents and solvent mixtures. G. caustic washings and sludge or wastewater and sludge from cleaning of tubs and equipment used in the formulation of ink from pigments.2. Waste organic solvent Halogenated organic solvents Includes the ff. driers. pyridine. spent halogenated solvents: Tetrachloroethylene. n-butyl alcohol. F:Inks/Dyes/Pigments/Paint/Latex/Adhesives/Organic sludge Aqueous based Includes all aqueous based wastes that also meet one or more of the subcategories Solvent based Includes all solvent based wastes that also meet one or more of the subcategories Inorganic pigments Includes all wastewater treatment sludge from the production of inorganic pigments Ink formulation Includes all solvent washings and sludge. soaps. toluene.1. Laboratory Operations & Safety Protocol 61 of 65 E503 E599 F601 F602 F603 F610 F699 G703 G704 V1:2010 .continuation (Table 5) Explosive and unstable chemicals Includes all wastes that are 1) capable of detonation or explosive reaction when subject to a strong initiating source or when heated under confinement. or 2) capable of detonation or explosive decomposition at a temperature of 20 o Celsius and Pressure of 1 atm. non-halogenated solvents: Xylene. methylene chloride. ethyl acetate. Trichloroethane. D502. Non-halogenated organic solvents Includes the ff. it also includes all still bottoms from recovery of these solvents and solvent mixtures. 1. carbon tetrachloride. and stabilizers containing Chromium and Lead. 2-ethoxy ethanol and 2 nitropropane and other non-halogenated organic solvents if they contain a total of 10% or more (by volume) of one or more of these solvents before use. ethyl benzene.1. 1. methanol. cyclo. chlorinated flouro-carbons if they contain a total of 10% or more (by volume) of one or more of the above before use. cresole. tricholorethylene. chlorobenzene. and D503. ethyl ether. Highly reactive chemicals Includes all other wastes that exhibit any of the properties described for D501.2 Trichloroethane.

and the chemicals listed in the Priority Chemical List. Containers that used to contain Polychlorinated biphenyl (PCB) are categorized as L406 and excluded from this sub-category. transport or treat more than 200 L of waste oil per day except vegetable oil and waste tallow H801 H802 I101 J: Containers Containers previously containing toxic chemical substances Waste containers that used to hold the toxic chemical substances listed in Classes A.continuation (Table 5) H.000 fowls or more. Putrescible/Organic Wastes Animal/abattoir waste Grease trap wastes from industrial or commercial premises I. Oil Waste oils Includes all wastes from animal feed lots containing an average of 100 or more animals. impermeable material in order to store hazardous wastes until such time that a proper disposal facility is available. J201 K: Immobilized Wastes Solidified wastes and polymerized wastes K301 Chemically fixed wastes K302 Encapsulated wastes K303 Laboratory Operations & Safety Protocol 62 of 65 V1:2010 . all waste from poultry farms with an average of 5. E. D. all waste from facilities that process an average of 2500 fowls or more. Includes all establishments that generate an average of 50 kg per day Includes all wastes from establishments that generate. sub-categories M504 and M505. and L. All wastes from commercial slaughter houses that slaughter an average of 500 or more animals per year . Wastes whose hazardous substances are physically immobilized by consolidation to reduce the surface area of the wastes in order to meet the waste acceptance criteria Wastes whose hazardous substances are chemically immobilized through chemical bonds to an immobile matrix or chemical conversion to meet the waste acceptance criteria Wastes whose hazardous substances are physically immobilized by enveloping the waste in a non-porous.

hexachlorobenzene. Waste pesticides other than M505. chlordane. heptachlor. bloods and body fluids). fetuses. mirex. toxaphene. and DDT.continuation (Table 5) L: Organic chemicals Wastes with specific nonhalogenated toxic organic chemicals Non-liquid waste containing the following: .organs. Recovered coolant containing chlorofluoro carbons (CFCs) or halons Wastes contaminated with PCB and waste products containing PCB. endrin.2-diphenylhydrazine benzene Waste chlorofluoro carbons (CFCs) and halons. Refer to CCO.1. infectious wastes and sharps Wastes containing friable asbestos. Includes all wastewater sludge from production of pesticides other than those listed in M505.Tri-butylin . Waste blue and brown asbestos fibers. L401 Ozone depleting substances L402 PCB wastes L406 M: Miscellaneous Wastes Pathogenic or infectious wastes Includes pathological wastes (tissues. Refer to CCO. dieldrin. Expired pharmaceuticals and drugs stocked at producers and retailers’ facilities. M501 Friable asbestos wastes M502 Pharmaceuticals and drugs M503 Pesticides M504 POPs (Persistent Organic Pollutants) pesticides M505 Laboratory Operations & Safety Protocol 63 of 65 V1:2010 . Waste pesticides listed in the Stockholm Convention (POPs Convention) such as aldrin.

Summary of Recommended Biosafety Levels for Infectious Agents1 Laboratory Operations & Safety Protocol 64 of 65 V1:2010 .Table 6.

17. 23.osha. 9. Anna Miren Gonzalez-Intal (VP Loyola Schools). Safety Practices in the Department of Chemistry. School of chemical Access Date: 6 June Access Date: 6 June 2002. 15. Dr. Researchers. Laboratory Safety Manual. Procedural Manual Title III of DAO 92-29. Environmental Health and Safety (http://web. 7. Ana M. What To Do When Handling Body Fluids. 11.labsafety. General Chemistry Lab (Ch 8) Course Outline & Lab Policies. 10 September 1996. OSHA Fact Sheet of Hazardous Chemicals in Labs (http://www. Memo to the Chemistry Faculty. Contact Lenses and Chemicals (http://pubs. Research and Teaching Assistants. Dr. 3.labsafety. Ma. Ateneo de Manila University. Access Date: 6 June “Hazardous Waste Management” DENR AO 36. February biosafetypage/section4. Laboratory Operations & Safety Protocol 65 of 65 V1:2010 . University of Tasmania . School of Science and Engineering). Health and Safety Office. Chemical Management and Inventory System Manual. 6 August 1979. on Fire and Evacuation Drill on Friday. National Research Council Recommendations Concerning Chemical Hygiene Laboratories (http://www. Judith Date Accessed: 6 June 2002. Cuyegkeng (Chair). February 1992. 2008 5. 8. 1997 Policies and Standards for B. on Operating Practices. University of North Carolina at Chapel Hill. Memo to the Loyola Schools Community. Chemistry (http://www. Access Date: 14 June 2010. University Chemical Management Plan.References 1. June 2002. University of Illinois.htm) Date Accessed: 21 November 2002. Less is Better.Launceston Campus.html) Date Accessed: 6 June 2002. Valera (Chair). 22.htm). University of North Carolina at Chapel Hill. Chemical Hygiene Plan. 4. Fabian M.1200 App A (http://www. January 2010. Dr.labsafety. 6. Memo to the Chemistry Faculty. Assunta Date Accessed: 6 June 2002. 2004 21. Undergraduate Teaching Lab Safety Contract Sample (http:// Date Accessed: 17 June 2010. Guide to Minimizing Waste in Laboratories (http://membership.acs. 20 July 1987. Staff. Proposal. 10. Students. 13. 14. 12. Dayrit (Dean. on Safety Policies. 19. 40 Steps to a Safer Laboratory (http://www. Javellana (Chair). Job description for Technical Assistant IV. 24. Health Hazard Definitions (Mandatory) –1910. in 18.osha. Date Accessed: 6 June 2002. 7 March Hazardous and Toxic Substances. Frequently Asked Safety Questions (http://www. 2. 2 May 2001.S.princeton. Princeton University.flinnsci.osha. 16. Francis Ted Limpoco. Microbiology Safety and Staff Induction Manual. 9 August 2002. University of Wyoming. 5 August Date Accessed: 6 June 2002. Occupational Safety and Health Administration (OSHA) (http://www. Nestor S.

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