Laboratory Operations & Safety Protocols

Chemistry Department Loyola School of Science & Engineering Ateneo de Manila University Version 2010

Preface to the 2010 Edition
In 2004, the government started to implement various laws, rules and regulations covering a broad range of activities related to the purchase, use, storage, waste management, and disposal of chemicals. The increased regulation has led the University to implement a common policy and procedure to address these various concerns. The Chemical Management and Inventory System (CMIS) was developed to address the need for accurate inventory, efficient purchasing, management, and compliance with various laws as well as the management of hazardous chemical wastes. The changes in the legal and regulatory climate have also contributed to the changing culture of safety which now puts emphasis on experiment planning, risk assessment, hazard consideration and chemical waste minimization. In this edition, information on different sections (i.e. Emergency Protocols, Use of Instruments and Waste Disposal) was updated. A section on the CMIS and Handling of Biological Samples was added. I wish to thank Ms. Mailyn Terrado for the section on Dealing with Biological Samples and Wastes. I also wish to thank Dr. Regina C. So and Dr. Fabian M. Dayrit for taking the time to proofread the manual. Jaclyn Elizabeth Santos 18 June 2010

Preface to the 2002 Edition


his manual is intended to be a safety reference document for lab workers at the Chemistry Department of the Ateneo de Manila University. It is mainly adapted from the Laboratory Safety Manual at the University of North Carolina at Chapel Hill, and the memo on Operating Practices issued by Dr. Ma. Assunta Cuyegkeng when she was Chair of the Department in 1996—practices that have served the Department well since the time of Fr. Schmitt. This manual provides basic information about hazards encountered in the lab and safety precautions to prevent accidents. It also contains the description of faculty, student, and staff roles in ensuring a safe working environment in the lab. It is not possible to cover all safety and emergency scenarios, but this manual should, nonetheless, serve as the starting point for the faculty in developing their own safety plans specific to the materials and procedures in their laboratories. It is often a sticky effort to balance convenience and control with regards to safety issues in the lab, but when all is said and done, the basic rules are alertness and vigilance, common sense, conservation of resources, and concern for other workers in the lab.

Francis Ted Limpoco
18 November 2002

Laboratory Operations & Safety Protocol

2 of 65



1. Introduction
1.1 1.2 1.3 1.4 1.5 Safety Awareness The Role of Faculty The Role of a Safety Officer The Role of Lab Technicians The Role of Students/Researchers

6 6 7 8 9

2. Emergency Protocols
2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 Dealing with Emergency Emergency Telephone Numbers Fire Emergency Chemical Spill Escape of Noxious Gases First Aid Medical Attention After First Aid Reporting Accidents and Injuries

10 11 12 13 14 14 15 16

3. The Use of Building & Facilities
3.1 3.2 3.3 3.4 3.5 3.6 Schmitt Hall Room Addresses Use of the Building Access Keys Special Rooms Use of Instruments

17 18 19 19 20 20

4. General Safety Principles
4.1 4.2 4.3 4.4 4.5 4.6 4.7 4.8 4.9 Overnight Operations Working Alone Eating, Drinking, and Smoking Housekeeping Warning Signs and Labels Shielding for Safety Compressed Gases Systems Under Pressure Cold Traps and Cryogenic Hazards

23 23 23 23 23 24 24 24 25 25

4.10 Glassware

5. Chemical Management and Inventory System
5.1 5.2 Chemical Management and Inventory System Controlled Chemicals

26 28

Laboratory Operations & Safety Protocol

3 of 65


3 Protective Clothing and Equipment 6.5 Waste Containers 7.1 Waste Characteristics 7.2 Waste Reduction 7.7 Clean-up Procedure 8.3 Form for Overtime/Overnight Lab Work Form for Reporting Lab Accidents Instrument Authorization Form 48 48 48 48 Figures Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 Figure 7 Figure 8 Figure 9 Figure 10 Figure 11 Lab Benches Fume Hoods Power.4 Labeling 7.4 Handling Toxic Materials 6.1 Hazardous Chemicals 6.1 A.7 Disposal of Chemical Wastes 40 40 40 40 41 41 41 42 8. Biological Samples: Handling and Waste Disposal 44 8. Handling of Chemicals 6.5 Laboratory Equipment 8.6.6 Waste Disposal 8. Gas.2 A.2 Storage 8.6 Handling Flammable Materials 6.8 Body Fluids 44 44 44 44 45 46 46 47 Appendices A.2 Routes of Exposure 6. Waste Disposal 7.4 Handling 8.3Transport 8.3 Handling Specific Wastes 7.6 Disposal to Sewerage System 7. and Water Lines Fire Extinguishers & Showers Floor Plans with Fire Exits Aspirator Set-up for the Clean-up of Mercury Spills Fire Alarm Compressed Gases CMIS’ Logical Computer-Hardware Set-up Overview of Requesting Procedure Overview of CMIS Requesting Procedures 52 52 52 52 53 53 53 54 54 55 55 56 Laboratory Operations & Safety Protocol 4 of 65 V1:2010 .7 Handling Explosive/Reactive Materials 30 30 32 33 35 36 37 38 7.1 General Information 8.5 Handling Carcinogens 6.

Tables Table 1 CMIS User-levels 57 57 Table 2 List of laws. rules and regulations which govern various aspects related to chemicals 57 Table 3 Table 4 Table 5 Table 6 Implementing Rules and Regulation for specific substances List of PDEA-controlled chemicals DENR Classification of Hazardous Wastes 58 58 59 64 Summary of Recommended Biosafety Levels for Infectious Agents References 65 Laboratory Operations & Safety Protocol 5 of 65 V1:2010 .

Check-in students at the start of the term.Chapter 1 Introduction T 1. b. 1. Every lab worker has a basic responsibility to himself and his colleagues to plan and execute lab operations in a safe manner. Please do not argue. As lab instructor. Common sense is still the guiding norm. (See Figures 1-5. and the locations of the valves for the gas. Orient students in the proper use of the lab facilities and instruments. Most of the rules/responsibilities stated here are nothing more than common sense and concern for others. Set a good example by being enthusiastic about safety. 3. the faculty should be in the laboratory for the entire laboratory period. You must consider not just yourself.2 The Role of Faculty The faculty is responsible for the administration of the safety program of the Chemistry Department. d.) 4. Thus. shower. eyewash. Laboratory Operations & Safety Protocol 6 of 65 V1:2010 . water. closed-toe shoes and apron). This attitude can lead to a false sense of security. and power lines. and evacuation plan. b. be alert to unsafe conditions and actions and call attention to them so that corrections can be made as soon as possible.” when it is obvious what you should do in a given situation. Maintain discipline and enforce the Department’s operational and safety rules.g. which frequently results in carelessness. This includes showing the locations of the fire extinguisher. a. Remind students to settle the lab breakage deposit immediately. As the saying goes: “An ounce of prevention is worth more than a pound of cure”. Over familiarity with a particular laboratory operation may result in overlooking or underrating its hazards. Ask if students have special medical conditions that may interfere with lab work/safety. and check-out the same at the end of the term. such as: a. Every instructor should: 1. “We were not told. Not entering the stockroom. 2. safety glasses. Inspect the equipment in the students’ and common lockers. Safety awareness can become part of everyone’s habits only if the issue of safety is discussed repeatedly and only if senior and responsible staff demonstrate a sincere and continuing interest in safety. Not eating or drinking during the lab. c.1 Safety Awareness he most important rule is that everyone involved in lab operations—from the highest administrative level to the individual workers—must be safety minded. and by observing all safety rules and wearing personal protective equipment (e. c. but also the other people involved— always consider their safety and convenience as well. NO SET OF RULES can cover all situations. Wearing the prescribed safety gear: safety glasses and aprons. Brief students on the lab operations and safety protocols.

Accidents resulting in medical treatment or observation should be recorded. the faculty not only guides the student in the direction of his research project. and take effective corrective action promptly. 3. 6. Manage the CMIS. Notify the parents/guardian of the injured person about the incident. and recommendations for the prevention of reoccurrence. 5. and by observing all safety rules (e. waste minimization and where appropriate. and Laboratory Operations & Safety Protocol 7 of 65 V1:2010 . Review and update safety protocol and procedures in the department. Carefully review all laboratory experiments for possible safety problems before experiments are assigned. 9. 6. regularly check safety facilities and equipment (i. 8. 7. A formal written report to the Chair should be made of all accidents. Report all accidents. spill kits) 4. be alert and vigilant for unsafe conditions. The mentor/supervisor should: 1. Coordinate with the University Physical Plant and Office of Adminitrative Service regarding the disposal of chemical wastes with a DENR-accredited waste transporter and treater. check that the system is working satisfactorily. Determine whether researchers need to work continuously after office hours or overnight. Anticipate and prevent accidents at all times. especially those that may pose safety hazards. In case of accidents/emergencies. fire extinguishers. Orient researchers with the instrument techniques and protocols.e. 3. stating the cause. eye wash stations. implement proper first aid measures and if necessary coordinate proper and speedy conveyance of the injured person to a medical facility. effect.3 The Role of a Safety Officer The safety officer is responsible for activities related to safety. chemical management. The mentor is responsible for checking-in researchers on the instrument or ensuring that they are checked-in by a competent/expert user of the instrument. aprons.g. 2. 10. Conduct a briefing on safety issues at the start of the class. etc. fire alarms. 8. compliance recommend appropriate improvements. Together with the laboratory supervisers and staff. Set a good example by being enthusiastic about safety. safety showers. 4. Regularly orient science majors (both undergraduates and graduate students. 2. The safety officer should: 1. As mentor/research supervisor. Orient the students on the chemical waste disposal procedures of the department. Discuss with researchers any safety risks involved in the experimental procedures that will be carried out and the materials that will be used in the research. redesign or replacement. 7. inspect often and intelligently.g. first aid kits. Train students in proper lab techniques. Orient researchers with the general operations and safety procedures at the Chemistry Department. storage and disposal of the Chemistry department. eyewear. no eating and drinking in the lab/stockroom) and wearing protective equipment (e. 1. where recommended).5. Conduct an inventory of chemical wastes generated by the department. hoods. Undertake an annual review of the laboratory experiments which are included in curricular offerings to consider aspects of safety. but also ensures that the student is not exposed to safety risks in doing his research. and ensure there are logistical provisions for their safety in such cases. especially those doing their thesis) research assistants and faculty members regarding chemical safety and chemical waste management and minimization.

b. Provide thesis students with chemical waste bottles. Inform the immediate supervisor well in advance of the need to order supplies before they run out. b. Provide technical services at Schmitt Hall. Report all accidents. Set a good example by being enthusiastic about safety. first aid kits. Regularly check current loads of aircons. such as: fire extinguishers. do various operations such as purification of solvents by distillation. Exercise supervision over students with regard to the stockroom transactions and general order in the laboratories. fine students for failure to observe laboratory rules or inform the lab instructor of any violations to enforce penalty.. and that these are placed in easily accessible locations in the laboratory. Under the supervision of faculty member.g. Manage the stock of chemicals and materials: keep records of materials. 4. a. According to established norms. 2. 7. Laboratory Operations & Safety Protocol 8 of 65 V1:2010 . investigate and repair simple electrical circuits and minor electrical breakdown. 10. Accidents resulting in medical treatment or observation should be recorded. 8. Ensure that the CMIS is updated. especially for laboratories. e. Assist the faculty in inspecting the student laboratory lockers/equipment at the beginning and at the end of the semester. a. effect. Prepare with accuracy chemicals and solutions for laboratory work. and deliveries. Together with the safety officer.4 The Role of Lab Technicians The lab technician and/or stockroom manager should also be present during the entire laboratory period to assist the faculty in managing the experiment and administering the safety procedures of the Department. first aid kits. Make plumbing repairs. c. Remind students to settle their lab breakage deposits immediately. etc. and sand for cleaning up spills. eye wash. no eating and drinking in the lab/stockroom) and wearing protective equipment (e. 6. fume hood. Prepare a periodic report (annually or as required) regarding the status of the chemical inventory and chemical waste of the department. assist the faculty/lab supervisor in implementing proper first aid measures and if necessary assist in the proper and speedy conveyance of the injured person to a medical facility. aprons. f. eyewear. stating the cause. and repairs on metal. This includes fire extinguishers. materials. and recommendations for the prevention of reoccurrence. In case of accidents/emergencies. mechanical repairs. Ensure that safety materials and paraphernalia are well in stock and replenished when used up. c. inspect all safety implements according to the recommended frequency. Dispense chemicals. d. Ensure that teaching laboratories are provided with properly-labeled chemical waste bottles. A formal written report to the Chair should be made of all accidents. 1. and plastic. Prepare chemical unknowns which must be carefully coded and recorded. Together with a trained professional. and equipment. supplies. The lab technician should: 1. glassware repairs. emergency showers. 5. 3. where recommended). Handle the renewal of licenses required by government authorities.g. and by observing all safety rules (e. and others. special containers for chemical and broken glass wastes. wood.9.

Goggles resting on your forehead are not in a position to protect your eyes. (See Figures 1-5. Read and prepare for remedies in case of exposure. and how to dispose of chemical waste properly. Aprons or some suitable protection must be worn at all times in the laboratory. and toxicity. or the lab technician in charge. b. horseplay. especially with hazardous chemicals. Contact lenses must not be worn. Keep personal belongings. and treat all chemicals with respect. books. from the work area. fire escape. c. No pranks.g. arms. Know the location of the fire extinguisher. Wear clothing that would protect your body against contact with chemicals. d. b. safety shower and eyewash. 6. 5. such as bags. and experiments. c. Report all accidents. consult with your lab instructor/supervisor. smoke. having read the experimental procedure in advance. or cellphones. Come in the lab prepared. Avoid unnecessary exposure to chemicals: a. d. Be informed about experimental procedures and appropriate safety concerns: a. Confine hair and loose clothing. whether major or minor. the student should be responsible for their personal safety when they are working in the lab. Do not perform unauthorized work. Laboratory Operations & Safety Protocol 9 of 65 V1:2010 . e. preparations. 2. or slippers. Never eat. Read and understand general safety procedures. 7. how to dispense the correct amount of reagents properly. Every student should: 1. or apply cosmetics inside the lab or storage areas. e. 4. reactivity. Any deliberate behavior that may compromise you and your co-workers’ safety may be grounds for your dismissal from that lab class/course. c.5 The Role of Students/Researchers The student/researcher is responsible for complying with the safety rules and operational procedures of the Chemistry Department.1. Never use your handkerchiefs or face/hand towels to wipe things in the lab. e. Research the hazards and physico-chemical properties of the chemicals used in the experiment. and those specific to your experiment. research supervisor. and face before leaving the work area. Wear clothing appropriate for work: Do not wear shorts. d. not preschoolers. drink. Consider lab work serious work. Wear proper personal protective equipment: a. Safety glasses must be worn inside the laboratory. When you are organized. or other acts of mischief.) Learn how to use instruments properly. you are less likely to commit accidents. Follow protocols for handling chemicals and for dealing with spills. Always wash hands. sandals. Never work alone. use medication. to the lab instructor. Although the Department aims to provide a safe working environment for its students. Follow hazard precautions. corrosiveness. b. If in doubt. The first thing you do in entering the lab is to put on your apron the last thing on leaving is to remove it. Keep a neat and clean work area. flammability. 3. first aid kit. You are university students. This should be done even if gloves are used.

Laboratory Operations & Safety Protocol 10 of 65 V1:2010 . 2.1 Dealing with Emergency What to do: 1. 4113 ) After office hours: • The University security personnel (local If the incident is out of control (e. area and immediately call for help. 4113) The following fire departments: o o o o 8363) Marilag Fire Sub-station (913 0125/510 9299) Pinagkaisahan Fire Sub-station (474 2695) New Era Fire Sub-station (931 9894/931 4956) Central Fire station (928 • University personnel (faculty. use fire extinguisher Immediately call for help. Who do you call for assistance? If the incident is small and contained. especially the one in-charge of the lab The lab technician.Chapter 2 Emergency Protocols 2.3 (Fire Emergency). or any Chemistry Dept. Alert others in the area of the accident. evacuate the Note: For fires. follow evacuation procedures under Section 2.) as above. rapidly spreading fire).g. Gonzaga (local 5624) 5102) The University security personnel (local 4111. etc. personnel (local 5624 or 5631) The Chemistry Department office (local 5620) 4111. If the situation is beyond your control and presents a danger to you. lab technician. disaster-assistance team captain of the building. immediately alert any of the following: During office hours: • • • Any faculty of the Chemistry Department. 3. 4112. 4112. Try to alleviate the immediate need in the situation without compromising your own safety: • • • Remove an incapacitated co-worker from the accident area Turn off fuel and power lines. immediately alert any of the following: • • • • The disaster-assistance team captain of the building (local The Fire Marshall at OAS.

University Gym) Direct Line local 4100 local 4101 local 4102 local 4104 426 5940 Infirmaries within Campus • • • • Health Service HealthDev (at the Social Development Complex) Jesuit Residence (ask for Infirmary) Loyola House of Studies (ask for Infirmary) local 5110 local 4630-33 local 3399 local 3650 Nearby Hospitals • • • • • Capitol Medical Center National Kidney Institute Philippine Heart Center St. Fire Departments • • • Quezon City Marikina Mandaluyong 928 8363 933 3076 5322189 Ateneo de Manila University • • Trunk line Telephone Operators 426 6001 local 4199 Chemistry Department Directory • • • • Department Office (Chair/Secretary) Schmitt Hall Stockroom SEC Stockroom Faculty Offices 5620 local 5624 local 5631 local local 5621-23. Luke’s Medical Center UP Health Service 372 3825 924 3601 925 2401 723 0301 981 8500 local 112 Laboratory Operations & Safety Protocol 11 of 65 V1:2010 .2. University Gym) Gate 2 Gate 3 local 4111 local 4112 local 4113 local Office of Administrative Services • Fire Marshall (Gonzaga Hall) 5103 Physical Plant Office • • • • • Administrator Secretary Switchboard Operation Center (South Wing. 5625-33 University Security Office • • • Main Office (North Wing. and must be updated annually.2 Emergency Telephone Numbers Note: These numbers are subject to change.

c. There are emergency exit routes posted on the wall of each room. S – Sweep from side to side. assume that the emergency is real until you are informed otherwise. Laboratory Operations & Safety Protocol 12 of 65 V1:2010 . etc. 2. b. 3. WARNING: Do not attempt to fight a fire that is spreading rapidly or if you are not sure how to operate the fire extinguisher. 5. Alert others in the area of the fire. A – Aim the nozzle. Do not re-enter the building unless you are given permission to do so.3 Fire Emergency What to do: 1. (See Figure 5. 4.g. containing CO2. (See Figure 4. Close windows and doors in your area. “Gas” extinguishers. Identify yourself Identify the kind of fire Identify the building and room number Give your telephone number Don’t hang up until the dispatcher has the needed information. Gonzaga hall (local 5103 or 5118) Quezon City Fire Department (928 8363 or 928 6535) The disaster-assistance team captain of the building (local a. offer first defense against flammable liquids or electrical fires without leaving a powder residue which could harm electronic equipment. and crawl if necessary. • • • • P – Pull the pin. d. Close the doors to confine the fire. Provide the following information 3. 1. use the portable fire extinguisher. 5. Meet the fire department outside of the building and provide information about any special hazards and other considerations. compressed gasses. 2. e.) To use the fire extinguisher. ovens.) 4. stay close to the floor. Evacuation Procedures. remember: Fire Extinguishers. If the fire is small and confined to its origin. small appliances. Immediately inform any of the following: • • • 5624) The Fire Marshall at OAS. Secure potential hazards in your area such as open flames. Leave the building using the nearest stair tower to reach ground level.2. e. S – Squeeze the lever. The fire may block your exit. If you encounter smoke. When alerted of a fire. and evacuate the building. for example. Most chemical laboratory fire hazards require dry chemical multipurpose extinguishers (ABC) which must be installed in hallways.

3. or an absorbent can be used to contain the spill. A quantity of solid not much greater than the volume of the liquid spilled should be sufficient. Avoid skin contact and minimize inhalation. Acids and Bases: Avoid contact with skin. Spill Clean-Up 1. Use pH paper to determine whether the acid or base have been neutralized. not highly corrosive. Exposed skin should be thoroughly washed with soap and water. as appropriate. If the material is not particularly volatile. 3. 2. If a volatile. Use. Place in a labeled plastic bucket or container. Dispose them properly. Continue flushing with water for 15 minutes or more. and turn off spark producing equipment. safety shower or eye wash fountain. closing the door and warning others not to enter the contaminated area. when necessary by opening windows or use a fan. Shut down all equipment and vacate the area until it is decontaminated. It is important to minimize the extent and possible effects of exposure to these hazardous chemicals. Wear appropriate personal protective equipment: goggles. Notify the faculty or lab technician in charge of the class (local 4. Use paper towels and dustpan to collect the spill residue. warn everyone immediately to extinguish flames. (See Spills of Specific Types of Chemicals below). you can add water to the spillsand mixture to act as dust-suppressant.4 Chemical Spill Dropping containers or otherwise spilling chemicals in the open lab can result in exposure to hazardous agents. especially for volatile and toxic spills. Rotate the eyeballs so that all surfaces are rinsed. 7. Seek medical attention (Health Service. gloves. 4. Spills of Specific Types of Chemicals 1. If the material is not reactive to water. respectively. and there is no fire hazard. 6. 5. 2. Report the incident to faculty or lab technician. However. 5110). Immediate Action 1. use an absorbent material that will contain the liquids. Neutralize acids and bases with solid sodium bicarbonate and citric acid.2. and if applicable. When the toxicity of the spilled material is unknown. 7. Do not leave paper towels or other materials used to clean up a spill in open trashcans in the work area. and respiratory protection. has a low order of toxicity. These should be laundered separately from other clothing before reuse. 8. sand. a minimum of 20-minute flushing with copious amounts of water is recommended. Everyone should leave the affected area. Use sand/soil to contain liquid spills. Any contaminated clothing should be removed and containerized. paper towels. Laboratory Operations & Safety Protocol 13 of 65 V1:2010 . even of concentrated reagents. Do not brush up solid spills since this may produce airborne dusts. flammable or toxic material is spilled. Ventilate the spill area. Forcibly hold the eyelids open as necessary. Mix neutralizing chemical into the spill. 9. 5. Add sand to contain the solid spill. treat the spill like a potential health hazard by avoiding exposure and seeking assistance from a trained personnel. local 5624 or 5631). proceed with clean-up operations. 6. adding some water to provide solvent for the neutralizing reaction. paper towels are not suitable for cleaning up flammable spills. For small liquid spills (<100 mL). For chemical splashes on the eyes. Check for and remove contact lenses.

. Place the source of gas in a hood (and make sure to turn the motor on).) Domestic vacuum cleaners must not be used. Water-insoluble oils must be washed off with soap and water. aniline vapor. Contaminated materials used to clean up the spill should also be placed in properly-labeled containers. Solvents may cause the oil to dissolve in the skin. Mercury spilled into floor cracks can be made non-volatile by amalgamation with zinc dust. accordingly. WARNING: Do not clean-up hydrogen fluoride (hydrofluoric acid. 2. Rotate the eyeballs so that all surfaces are rinsed. Very much lighter vapors will rise. Chemicals in the Eyes: A minimum of 20-minute flushing with copious amounts of water is recommended. burn ointments. etc. First Aid for Specific Types of Accidents 1. Final rinse is clear water. and close off the room from the rest of the building. Rinse mop and bucket. Check for and remove contact lenses. Chemicals on the Body: Flush areas copiously with water under safety shower or under the sink. 2. Leave the room in either upright or crawling position. evacuate personnel. The situations described herein are the ones of particular concern in a chemistry laboratory. In any event. open windows. 3. WARNING: A mask may protect the wearer against inhalation of noxious gases. Laboratory Operations & Safety Protocol 14 of 65 V1:2010 . Keep victim warm. spilled mercury should be cleaned-up immediately and thoroughly using an aspirator or vacuum device. HF) with silica-containing materials such as sand or vermiculite. or by adding a mixture of finely powdered sodium thiosulfate (85 g) and powdered EDTA (15g). cutting or tearing them if necessary to get them off rapidly. thus increasing the damage to the skin and promoting poisoning through the skin. The stockroom must also be ready to provide first aid supplies: bandage. 2. Forcibly hold the eyelids open as necessary. antiseptic wash. Heavy vapors (MW >>29) will lie along the floor. Quickly remove chemically soaked clothing. (See Figure 6. Take (do not send) the victim to the Office of Health Services (local Sciences Building). Watch victim carefully for shock. 2. Modesty should not deter removal of clothing in a true emergency. WARNING: Do not use solvents to wash off offending oily chemicals. Social 2. mopping should follow. refer to a more extensive first aid manual. etc. but be warned against absorption through the skin of such substances such as HCN gas. Do not use sulfur to cover mercury since this will just complicate disposal.6 First Aid All members of the Chemistry Department must undergo a yearly first aid and cardiopulmonary resuscitation (CPR) course and should be ready to apply first aid measures to an injured person in a lab. etc.5 Escape of Noxious Gases Immediate Action 1. 5110. For more general concerns. and the burning and poisoning through skin by HF gas.After neutralizing. poison remedies. Mercury: Because of the high toxicity of mercury vapor.

Treatment with ice water is not recommended for large-area burns. as 3% boric acid (remedy for base) or 5% bicarbonate (remedy for acid). trembling. 3. Keep victim warm. Cover burned areas in 3% boric acid. a victim should not drive a car for several hours after a severe injury. it seals the chemicals under the greasy coating. or better. Shock can be fatal! Be on the lookout for it. 5110.7 Medical Attention After First Aid After being administered first aid. Keep moist. Watch victim carefully for shock. Social Sciences Building). hence the advantage of using the safety shower. magnesium sulfate. pounding heart. and first aid precautions to follow in case of chemical poisoning. WARNING: Do not apply greasy burn ointment to chemical burns. The cold shower will suffice. Watch victim carefully for shock. cold sweat. Laboratory Operations & Safety Protocol 15 of 65 V1:2010 . Social 4. WARNING: Application of cold to large areas of the body produces shock. 1. do not apply burn ointment. even though at the time you dismiss them they assure you they feel fine and are quite ready to go alone. Watch victim carefully for shock. have shock victim lower head between knees. Take (do not send) the victim to the Office of Health Services (local Sciences Building). The supervisor (faculty/lab technician) or a co-worker must accompany the injured person in seeking medical treatment. 5110. the injured person must be taken (not sent) directly to the Office of Health Services (local 5110. lab coat. Also refer to first aid wall charts. and difficulty in breathing. etc. jittery talk. There is always the possibility that they may collapse en route. Keep moist with neutralizing solution soaked on gauzes. heavy sweating. All these are signs of trouble and require immediate medical attention. Keep victim warm. Remedies include: egg albumin. extreme dullness or sluggishness. Shock: Accident victims experience shock. and shallow breathing. It may be the most dangerous result of an accident. Chemical Poisoning: Get poison remedies/antidotes from the stockroom manager or lab technician. jacket. Keep victim warm. Symptoms of shock include: cold sweat. Take (do not send) the victim to the Office of Health Services (local Sciences Building). extreme paleness of the face. 5110. weakness of the arms and legs. Watch victim carefully for shock. Signs of Danger: Watch the victim for blood leaving the head (face turning white) or excess blood in the head (face turning red). etc. shivering and chills. 2. very rapid pulse.g. Social 2. and tannic acid. have him lie with head lower than the body. Social Fire Burns: Rush victim under safety shower or smother fire on victim with a blanket (e. nausea. vomiting. Burned areas should be cooled immediately. The antidotes required. are usually printed in red letters on the bottle labels. mustard powder. Soak affected part in ice water for 20 minutes to reduce deep burning. Keep victim warm. Only very minor burns should then receive burn ointment. Also. On most fire burns. To restore blood to the head. shallow rapid breathing. charcoal. Take (do not send) the victim to the Office of Health Services (local Sciences Building).). Transporting the Injured Person: No one should be sent to seek medical attention without accompaniment. nausea.Follow water-washing with the correct neutralizing solution.

Accident reports (see Appendix A. hospitalization. Give oral stimulant (hot tea or coffee) if conscious. neutralizing chemicals. 2. apoplexy. Only give inhalation (aromatic spirits of ammonia or amyl nitrate) in the mouth only if the victim is unconscious. If he vomits. be sure his air passages do not become blocked. to the building manager or lab technician so that the equipment can be recharged of replaced. 2. for demonstrating whether faculty/staff were negligent in preventing the accident or careless in subsequent treatment of the victim. If the injured person is not able to do so. File injury/accident reports with the secretary of the Chemistry Department. 3. It is the student’s/researcher’s responsibility to notify his supervisor immediately of any work-related injury or illness. Laboratory Operations & Safety Protocol 16 of 65 V1:2010 . abdominal injuries. fractured skull. It is also the supervisor’s responsibility to file an injury/accident report with the Chemistry Department. or hemorrhage of the head. WARNING: It is still best to take the victim to see a physician first before giving any oral remedy. apoplexy. Report the Use of Safety Equipment: Immediately report the use of eye wash. and not at all in case of fractured skull. 1. elevate the head rather than lower it! This may occur in the case of sunstroke. for establishing a claim for damages of insurance benefits. fire extinguishers. 3.8 Reporting Accidents and Injuries All personal injuries and accidents should be reported in order that corrective action may be taken to minimize the probability of recurrences. Any injury resulting in lost work time. etc. Do not give stimulant until bleeding is controlled. or medical treatment in excess of first aid will be investigated by the Chemistry Department to determine liability or the possible need for corrective action. showers. or sunstroke. a coworker should notify the supervisor as soon as possible.WARNING: If the face is flushed or red.2) are of the utmost importance for correcting procedures. Keep the victim warm. It is the supervisor’s (faculty’s) responsibility to ensure that the student receives prompt treatment of the injury by obtaining first aid or assistance to medical treatment. for establishing legal responsibility for an accident.

finances. which in the past have served the Department well. This set-up is also based on as sense of trust that each one will do his share in ensuring these practices. The building is named after Fr. The judicious use of resources—energy. It has saved the Department from serious accidents because of the emphasis on safety at the workplace. Schmitt. The heart of an effective working set-up is the responsibility and discipline of users of the building and its facilities. you will see that the good of many outweighs individual convenience. who was instrumental in its design and construction. William J.Chapter 3 The Use of Building & Facilities 3. and effort—has led to the conservation of resources. It has given us the edge on effective equipment maintenance because malfunctions were reported at once. It has spared us from serious theft because security measures were taken in earnest and implemented. While in some cases some convenience is sacrificed. the Ateneo Chemistry Department has been able to maintain a good and effective working set-up which has yielded beneficial outcomes. Over the years. are maintained to a high level of compliance. and its long tradition of high standards in safety. Laboratory Operations & Safety Protocol 17 of 65 V1:2010 . with a laboratory extension for undergraduate labs on the 2nd floor of the 3rd wing of the Science Education Complex (SEC-C).1 Schmitt Hall T he Chemistry Department is housed at Schmitt Hall (formerly the Chemistry Building).

Offices a. e.116. e. 301 201 109. Lecture/Seminar Rooms 3.115. Service Rooms a. Department Office Faculty Offices Faculty Lounge 112 101. c. Stockroom Balance Room Instrumentation Room AFM Room Multimedia Room Glassblowing Workshop 208 308 310 204 111 4th Floor 4. b. f. d. c. c. Teaching Laboratories a. d. 2nd Floor SEC-C 307 309 6.3. f. c. 2nd Floor SEC-C 209. 205 2. b. b. 115. b. b. National Chemistry Instrumentation Center (NCIC) a. Research Laboratories a.2 Room Addresses 1. also houses the AAS and HPLC 207. g. c. d. Director’s Office Staff Office GC-MS Lab NMR Lab 102 104 103 105 5. General Chemistry Lab Organic Chemistry Lab Analytical Chemistry Lab Physical Chemistry Lab. Organic Synthesis Lab Natural Products Lab NCIC Wet Lab Biochemistry Research Lab Polymer and Materials Lab Polymer/Biochemistry Wet Lab Electrochemistry Lab 203 202 206 304 302 305 303 Laboratory Operations & Safety Protocol 18 of 65 V1:2010 . d.

1). d. faucets. Tables should be wiped clean. and the Office of Administrative Services must be informed..m. ovens and other lab equipment (unless overnight run is explicitly allowed by senior faculty). plates. c. Toilet and shower room should always be maintained in a clean. etc. For classes. b. glasses. It is a basic rule in any good chemistry lab to have at least 2 people in the lab. The last person to leave a laboratory should make sure that the following are closed/locked/turned-off properly: windows. 3.m. coffee maker. 2.4 Access Keys 1. Anyone sleeping in the faculty lounge should be up by 6:30 a. and locked by the utility man at 6:00 p. should be submitted to the secretary of the Chemistry Department at least 2 days before the event. He. As a rule. The building is opened to the public by the building manager by 7:00 a. Only faculty members and the lab technicians have access to keys for the main door. In case the building manager is not yet around by this time. a. faculty or staff may open the main door and the classrooms. a. e. gas lines (LPG. and the sub-master key (for labs). etc. c. Always lock doors after every use.m. d. However. Never assume that someone else will do these for you. the opening and locking of rooms is the main responsibility of the faculty handling the class. A written request form (Appendix A. 5. If an experiment requires longer than 15 hours. Try to work out a schedule that will go from 6:00 a.3. Sink should be properly cleaned. Researchers and graduate students may loan keys to the special labs (instrumentation. to 10:00 p.m.m. Food supplies should be sealed and arranged properly. This will allow the office to inform the administration and security. During these times all doors should be unlocked for safety. should be responsible for obtaining permission and getting access to the main door. Sign at the Night Log. Laboratories should only be unlocked when there are lab classes or when an experiment has to be performed in that lab. lights. Anyone staying beyond 10:00 p. e. but both the lender and Laboratory Operations & Safety Protocol 19 of 65 V1:2010 . For research assistants and students doing research. c. The faculty lounge should be kept clean and orderly. to ensure the safety of lab workers. b. a.). polymer and materials. so that the entry or exit from the lab is easy in an emergency. faculty and staff can stay inside the building until 10:00 pm. b. It is for your own safety. Bathroom tissue should not be removed from the toilet. At least two people should stay for overnight work.m. N2. respectable condition.3 Use of the Building 1. their respective offices. 2. overnight stays are discouraged. natural products labs) from a faculty member. they have the responsibility of seeing to it that the labs used are properly locked after use. b. 4. aircon. 3. endorsed by the mentor/supervisor. All used utensils. Lights should be turned on only in the appropriate areas of work. Overnight stays in the building should be processed with the Department office: a. overnight stay needs to be approved by the mentor and department chair. and doors. should be cleaned. hoods.

Report any noticeable malfunction of the instrument immediately by reporting it to the Department office. If no sign is placed or if the time is over. either or both the aircon or the dehumidifier must be turned on (for the FTIR and the AFM). This assumes responsibility in the use of the keys. anyone with a key to the room should lock it and report the matter to the Department office. etc. All users must familiarize themselves with the operation of the instrument. and AFM room. As a rule. Faculty offices should never be left unlocked. Most major instruments have voltage regulators. turn on the aircon. 4. but keep them closed. Keep the doors locked even when leaving the room for a short while. 2. Access keys are loaned to you in trust.)All users must sign the instrument logbook. and there are no other people using the room. and shut-down routines. Understand the instrument thoroughly. Equipment and accessories used should be kept in proper order. 4. they should be locked at all times. the physical chemistry lab. (The student’s name must be marked in the Instrument Authorization Table posted in the analytical chemistry bulletin board.the one who borrowed are accountable for the keys. 3. Access/use of any instrument requires check-in and authorization by faculty-in-charge. the AFM room. 3. Report immediately to the person-in-charge or to the Department office when instrument supplies are running low (e. ink. SHORT NOTES are provided as supplement to the manuals. 2. When using the instrumentation room.g. the research labs. chart paper. When using the balance room. indicating the time they are returning. Please be considerate of faculty members who hold offices in some of these special labs. Unlock the doors. Verify the power requirements of the instruments (110 or 220V) before plugging it into the outlet and turning it on. Return the items to where they were found. no equipment (major nor minor) can be taken out of the building. 4. warm-up. c. they should take the responsibility of informing the faculty involved or the stockroom technicians when they are finished. 10-15 min). 3. A student cannot use the instrument alone unless he/she is authorized to use that instrument. Do not attempt to fix the equipment yourself. b. so that no fatal mistakes are committed. When rooms are unoccupied. 6. Do not unplug them from their voltage regulators. 3. If they are going to leave the room for a short period of time (e. 3. they have to put up a sign on the door. Review the instructions before the operation.6 Use of Instruments 1. without explicit approval of the Chairperson and a senior faculty member.5 Special Rooms 1. Any loss of keys should be reported immediately to the Department office. Creating duplicates on your own is strictly prohibited. and glassblowing workshop: a. When using the polymer and materials lab. 5.g. especially the power-up. Laboratory Operations & Safety Protocol 20 of 65 V1:2010 .). The performance of the balances are dependent on temperature. This is needed to prevent further damage and to ensure speedy repair. These cannot be moved/relocated without the explicit approval of the Department Chair. Keys should never be left lying around in the rooms. If students are using the room and do not have easy access to the key.

Laboratory Operations & Safety Protocol 21 of 65 V1:2010 . If someone is using it. 6. The Faculty-in-charge signs the certificate of authorization if the student has demonstrated competency in using the instrument during the next sessions. The faculty-in-charge inspects the following during check-out: a. Short Notes of the Procedure e. The Instrument manager updates his list of students authorized to use the instrument. the faculty-in-charge must discuss the following to the student: a. For computers connected to the instrument. Procedure for instrument check-out 1. Only students who passed the safety exam will be allowed to use the instrument. water bath. make sure he/she understands that you are leaving and you are turning over the responsibility of shutting down to him/her. oven. the student must schedule an instrument check-out with the faculty-in-charge. Working environment 3.g. The faculty-in-charge signs the check-in part of the instrument authorization form as proof of check-in. computer) turned on. comments) if you are leaving something (e. The student who wishes to use the instrument must fill-up the instrument authorization form (Form A. 9. AAS and IR spectrophotometers). Leave a note (name. Lock the lab door immediately. 10. instrument. The date of authorization must be different from the date of check-in. The student’s mentor must approve the student’s plan to check-in. The student submits to the instrument manager for filing and documentation the instrument authorization form filled up to the certificate of authorization part. a general check-in will be scheduled every July of each year. Important Reminders and Considerations 4. students who wish to use it must request the technician or a faculty member to open the lab for them. 8. If the instrument room is locked. Safety and Precautionary Measures d. 3. He/She can get a copy of the form from the instrument manager. he/she can ask a person who is already authorized to use the instrument to analyze his/her samples. During the check-in. Instrument General Working Principles b. 2. 2. time. date. Procedure for Instrument Check-in 1. When done. 5. Equipment that is left on without any note will be shutdown after 6:00 pm. Instrument parts b. The student must already have actual samples to run during check-in.3).7. The student and faculty-in-charge schedules the next session. Consumables d. All graduating students must make sure that they have checked-out of the instruments they are authorized to use for clearance purposes. The student schedules the instrument check-in wth the faculty-in-charge one week before the actual check-in. ON TH SPOT checking-in is not allowed. For the commonly-used instruments (UV-Vis. Only students who will be using the instrument more frequently will be checked-in. If the student will be using the instrument only once or twice. instrument use has priority over other use. analysis. After the last use of the instrument. Instrument accessories c. Instrument Parts c. do proper shutdown.

3. the department chairperson discusses with the requesting party the department policies on the use of instrument and the charges: a. The department chairperson together with the faculty-in-charge and/or the instrument manager decides whether to grant the request or not based on the instrument schedule. If the request is granted. Mentor will be notified. Mentor will be notified.Penalties Any student who fails to follow the policies and guidelines will be subject to the following penalties: First Offense: Oral reprimand from the instrument manager and the department chair. He/She must include in the letter the purpose of the instrument use. Procedure for instrument use by outside parties 1. feasibility of the analysis and availability of the needed accessories. The person writes a letter addressed to the department chairperson requesting to use an instrument. Third Offense: One week ban in using the laboratory. the department chairperson assigns to the available faculty-in-charge the granted requests. Mentor will do necessary action. If the requesting party agreed to the terms and conditions. Operator fee 4. He/She must include as an attachment the details of the procedure and instrument conditions. Laboratory Operations & Safety Protocol 22 of 65 V1:2010 . Second Offense: Oral reprimand and community service. 2. Instrument use and b.

The supervisor has the responsibility for determining whether the work requires special safety precautions. Other signs should be posted to show the locations of safety showers. Operations should be designed to be safe. Temporary holding containers should be clearly marked. and chemicals and equipment must be properly labeled and stored.5 Warning Signs and Labels Laboratory areas that have special or unusual hazards must be posted with warning signs. and fire extinguishers. 4. and cold rooms. Experiments known to be hazardous should not be undertaken by a worker who is alone in a laboratory. such as fire hazards (See Figure 8). 4. The safety. it is essential to plan for interruptions in utility services such as electricity. handled. chemicals should not be brought into designated eating areas.1 Overnight Operations If laboratory operations are carried continuously or overnight. security guards may be asked to check on the lab worker. 4. smoking materials. are likewise not to be used for food storage. if appropriate). Waste containers must be labeled for the type of waste for which they are intended. In any case. safety performance inevitably deteriorates. such as having two persons in the same room during a particular operation.2 Working Alone Generally. and inert gas.Chapter 4 General Safety Principles 4. and cosmetics is a potential route for exposure to toxic substances. 4. Likewise. Glassware and utensils that have been used for lab operation are not to be used for food or beverages. Wastes are to be deposited in appropriately labeled receptacles. Stairways and hallways should not be used as storage areas. Access to exits. and consumed in an area free of hazardous substances. Laboratory Operations & Safety Protocol 23 of 65 V1:2010 .and hazard-sign systems in the lab should enable a person unfamiliar with the usual routine of the lab to escape in a emergency (or help combat it. emergency equipment. and controls. laser operations. snacks and lunches are not to be brought into laboratory areas. etc. Coffee. it is prudent to avoid working in the laboratory alone. unless it is explicitly allowed by the Chair and senior faculty. biological hazards. drink. soft drinks. exits. ice chests. Extinguishers are to be labeled to show the type of fire for which they are intended.4 Housekeeping There is a definite relationship between safety performance and orderliness in the laboratory. Food should be stored. Lab refrigerators. the laboratory lights should be left on and an appropriate sign should be placed on the door or near the set-up. Under normal working conditions. Standard signs and symbols have been established for a number of special situations. Drinking. water. eyewash stations. and Smoking Contamination of food. Never leave a set-up unattended overnight. Alternatively. When housekeeping standards fall. Work areas are to be kept clean. arrangements should be made between individuals working in separate laboratories outside working hours to crosscheck periodically. and plans should be made to avoid hazards in case of failure.3 Eating. Chemicals that are no longer needed should not be permitted to accumulate in the lab. must be maintained free from obstructions. Cleanup should follow the completion of any operation of at the end of each day.

Acceptable methods of support include: wall-mounted or bench-mounted gas cylinder brackets.. grease. elevated temperatures and/or pressures.4. may result in the rapid and violent oxidation of normally non-reactive materials. an apparatus that is a closed system unless it is designed and tested to withstand pressure. is prohibited. if desired. cylinders weighing less than 50 lbs may be carried. gauges. or contamination. Although oxygen is quite safe under normal temperatures and pressures. 6. graphite. Gas cylinders must be used in an upright position and clamped securely at all times. a regulator for oil-pumped nitrogen could produce a serious explosion if subsequently used for oxygen. Laboratory Operations & Safety Protocol 24 of 65 V1:2010 . even though the cylinders are secured to a dolly.g. and 3. Toxic and poisonous gases must be used only in fume hoods or other enclosures vented directly outdoors. 2. Compressed gas cylinders must be supported and firmly restrained at all times. 4. Whenever a reaction is attempted for the first time (small quantities of reactants should be used to minimize the hazards). i.e. For example. Moving by spinning. A cylinder connected to a piece of equipment and properly supported is considered to be in use. 7. etc. If the reaction cannot be opened directly to the air. sliding. Pressurized apparatus should have an appropriate relief device. pipes. whether full or empty. 5-10 times more material). 4. rolling. regulators. 2.8 Systems Under Pressure Reactions should never be carried out in. 4. All oxygen valves. nor heat applied to. Appropriate first aid and antidote information and supplies must be provided and clearly marked at room entrances. Whenever a familiar reaction is carried out on a larger than usual scale (e. Smoking is not permitted in the area where flammable gases are used or stored. or any other oxidizable substance. the use of adapters is prohibited. Appropriate dollies are to be used to move cylinders weighing more than 50 lbs. Shields are to be placed so that all personnel in the area are protected from hazard. Pressure regulators and gauges must be compatible with the cylinder valves.7 Compressed Gases The following rules summarize a few of the basic guidelines for the use and storage of compressed gases: 1. 8. Whenever operations are carried out under non-ambient conditions. For movement within shops and laboratories.6 Shielding for Safety Safety shielding is to be used for any operation having the potential for explosion such as 1. an inert gas purge and bubbler system should be used to avoid pressure build up. 5. 3. due to the oil residue. chains or belts anchored to walls or benches (see Figure 9 in the Appendix). and fittings must be scrupulously free of oil. The pressure regulators must be removed and valve protection covers replaced before moving cylinders. Gas cylinders must have the valve protection cover in place (see Figure 9 in the Appendix) except when in use.

Careful handling and storage procedures should be used to avoid breaking glassware. Equipment such as Dewar flasks should be taped or shielded. can cause severe burns if allowed to contact the skin. Do not store in a refrigerator or freezer. Dry ice should be added slowly to a liquid portion of the cooling bath to avoid foaming over. Proper instruction should be provided in the use of glass equipment designed for specialized tasks. (Small pieces should be swept up with a brush into a dustpan. Adequate hand protection should be used when inserting glass tubing into rubber stoppers or corks or when placing rubber tubing on glass hose connections. Tubing should be fire polished or rounded and lubricated.4. Laboratory Operations & Safety Protocol 25 of 65 V1:2010 . which leads to an explosion hazard. (For example. and suffocation can result. separatory funnels containing volatile solvents can develop considerable pressure during use. Workers should avoid lowering their head into a dry ice chest: carbon dioxide is heavier than air. Wetting the glassware should also facilitate the process of inserting glass tubing into rubber stoppers. Vacuum-jacketed glass apparatus should be handled with extreme care to prevent implosions. Only glassware designed for vacuum work should be used for that purpose. They. Hand protection should be used when picking up broken glass. Glass-blowing operations should not be attempted unless proper annealing facilities are available. 4.) Glassware which is to be heated should be Pyrex or a similar heat-treated type. and surfaces they cool.) Broken glassware should be disposed of in a special container marked BROKEN GLASS. which can represent unusual risks for the first-time user.9 Cold Traps and Cryogenic Hazards The primary hazard of cryogenic materials is their extreme coldness. Neither liquid nitrogen nor liquid air should be used to cool a flammable mixture in the presence of air because oxygen can condense from the air. The use of plastic or metal connectors should be considered. Insulated gloves and a face shield may be needed when preparing or using some cold baths.10 Glassware Accidents involving glassware are a leading cause of lab injuries. Appropriate insulated gloves should be used when handling dry ice. and hands should be held close together to limit movement of glass should fracture occur.

Different user-levels Only selected accounts can access restricted areas of the system. accurate inventory. students) No accounts are assigned to Level 1 users.e.and chemical resistant barcode sticker. PDEA and PNP.Chapter 5 Chemical Management 5. Features classification of wastes. Information database Supplier and chemical information are stored in the database. 3. Back-up and Recovery Mechanisms CMIS has features which provide means to back-up the database and restore all data should the system fail 7. Tracking of controlled chemicals under the EMB. which provide quick access to: ü Physico-chemical information ü Handling and storage conditions ü Health and safety information ü Disposal considerations 4. stock-level. Level 1 users may: 1. 2. chemical expiration. CAS number.1 Chemical Management and Inventory System (CMIS) The Chemical Management and Inventory System (CMIS) combines a web-based inventory system and barcode scanner to provide a means for efficient updating and query of chemical stock levels. Hazardous Waste Inventory System The CMIS is linked to the Hazardous Waste Inventory System (HWIS) which allows monitoring of chemical waste generation and disposal. PDEA and PNP System not only allows monitoring of chemicals on-stock but also controlled chemicals under the EMB. Obtain chemical information on chemicals (i. Using the CMIS. threshold reports. Supplier details and hazard codes) Laboratory Operations & Safety Protocol 26 of 65 V1:2010 . Exportable Reports Reports (i. Safety Data Sheets (SDS) bank Safety Data Sheets (SDS) for chemicals are stored in the system.ateneo. Scanner-server synchronization Chemical data in the barcode scanner are easily synchronized with data in the server 8. Reports are easily prepared using the system’s Reporting Service 5. chemical movement) can be exported as comma-separated version (CSV) files which can be opened in Microsoft Excel. IUPAC name. management and compliance with various laws. The system may be accessed by any computer within the campus (http://cmis. efficient purchasing. consumption. Each chemical bottle is provided with a tear. Access to the different features depends on the user-levels as shown in below: User Level 1 (Guests. CMIS is meant to address the need for accessibility of information. Open Office Spreadsheet or any text-capable reader 6. generation of reports (i. chemical waste disposal and chemical waste movement) User Levels CMIS has four (4) user-levels (Table 1).e. The system of tracking is container-based. Check availability of chemicals in the department 2.

3. 2. Proceed by clicking on the “Click Here to Request for a Chemical” link. 3. The system will then provide the requestor with an update on the status of his/her request. click on the “Click here to View Existing Chemical Request” 2.” 8. Laboratory Operations & Safety Protocol 27 of 65 V1:2010 . The Request Home Page will then be shown. 6. print the form and have it signed by your thesis mentor. Please take me to check out. Level 2 users also have access to information regarding the stock level and stock location of the chemicals. A page will appear where the Chemical Name or IUPAC Name or CAS Number of the chemical can be entered. In addition to the features available to a level 1 user. The system will then ask for the chemical’s common name. To check for stock levels of chemicals using the Inventory System. Input the reference number issued upon checked out from the Request Wizard.” 7. Any special instructions for this particular chemical? – Special instruction on how the chemical is to be handled or stored should be noted here.3. the system will then ask for the following: Concentration – what is the concentration of the chemical that you need? Grade – what is the grade of the chemical? How much do you need? – Input amount is in liters or grams. Requestor Name – the name of the requesting party (who made the request?) Requestor Department / Company – the department or company affiliation of the requestor Purpose – the reason for the request (where will the chemical be used for?) Request Date – date of the request (when was the request made?) 4. click on the “Request” Link from the Home Page. Click on the Chemical’s Common Name link to show the information about the chemical. Access (Material) Safety Data Sheets ((M)SDSs) of chemicals 4. 5. Stock Inquiry 1. click on “I do not need any more items. Research Assistants (RAs) and junior faculty) An account and temporary password is given to Level 2 users. Show the signed form to the stockroom personnel and claim chemicals. Requests for other chemicals can be added to current request by clicking on “Click here to add more chemical requests. If request has been approved. If the chemical is on stock. 3. User Level 2 (Teaching Assistants (TAs). To end request wizard. Provide the chemical’s common name. click on Stock Inquiry. Fill up the form with the required information to proceed. 4. List of requested items may also be viewed by clicking on “Click here to review requested items. Users may change the password using the Password Management feature of the system. To Request for Chemicals. To check the status of chemical requests. Request for a chemical Steps in requesting for a chemical (See Figure 11 in the Appendix for the overview) 1. Checking status of request 1. 2.” The check-out page will then show the reference number which can used by the requestor to review status of request.

e. consumption. CMIS manager. A “Back-up succeeded” message will then appear on the screen. rules and regulations covering a broad range of activities related to the purchase. User Level 4 (Dean. Sub-pages for the selected field will then be displayed. Level 4 users have access to all of the features of the CMIS. preparation of a back-up of the database and synchronization of the server and barcode scanner. 5. Department Chair. Lab technicians. Click on the “Synchronize All” button to perform all operations in sequence.). To save the back-up file. Different implementing agencies (i. 2. Laboratory managers. The safety officer together with the system administrator of the MIS office is in-charge of the maintenance of the database system. technicianin-charge of CMIS) An account and temporary password is also given to Level 4 users. thresholds etc. Level 3 users also have access to the reporting services of the system. right-click on the “The Back-up file” link and choose “Save Link As…” or “save Target As…” Synchronization of server and barcode scanner The sync station software is used to synchronize the barcode reader’s database with the server’s database.User Level 3 (Department secretary. 4. “Back-up. Click “add ____. chemical brand. 3. Then click the link. encode additional data (e. storage locations.” 2.2 Controlled Chemicals In 2004. Senior Faculty) An account and temporary password is also given to Level 3 users. Open the Sync Station software and ensure that all databases are connected.g. EMB. To synchronize the server and barcode scanner. Level 4 users also have access to the hazardous waste inventory system and the administrative functions of the system. This includes management of CMIS accounts. chemicals and thresholds) on the home page. Setting up of additional data such as supplier information. 2. and disposal of chemicals. In addition to the features available to a level 2 user. These can be grouped under the general headings of environment. storage and disposal of the following controlled chemicals: Laboratory Operations & Safety Protocol 28 of 65 V1:2010 . use. To add new data. 5. chemical grade. supplier information. storage. hazard codes and threshold levels may be done by clicking on the appropriate links (i. PNP and PDEA) monitor the purchase. dangerous drugs and explosives (Tables 2 and 3 in the Appendix). Users may change the password using the Password Management feature of the system. click on the link ”Back-up and Recovery” link on the Administrator Functions Section. 1. The system will then display a dialog box once all operations are finished. new storage locations. the government has started to implement various laws. movement. Preparing a back-up of the database 1. Suppliers. storage locations. chemical brands. Users may change the password using the Password Management feature of the system. close all active application in the barcode scanner. In addition to the features available to a level 3 user. hazard codes. add/update chemical bottle inputs.” button to submit and save new data. click on the “add ____” link to display the form where new entries can be supplied. To back-up the databse. Setting up of additional data to the CMIS 1.e. waste management. Place the scanner in the dock and wait for “active sync” to finish synchronization.

administration. delivery. PNP PNP monitors and regulates the importation. Semiannual reports on the purchase and consumption of PDEA-controlled chemicals are also submitted to PDEA. He/She must include in the letter where the chemical will be used as well as the amount of the chemical being requested. delivery. The person checks availability of chemical using the CMIS. This license is renewed yearly. Monthly consumption reports are also submitted to PNP. The university is required to obtain a Purchaser’s license from PDEA. distribution. sale. upon presenting the official receipt from the Cashier. from their importation. the person then writes a letter addressed to the department chairperson requesting to purchase the chemical. Requesting party may then pick-up the requested chemicals from the stockroom. distribution. If the chemical is available. Since the department uses these compounds for laboratory classes and research. manufacture. possession or use of explosives and explosive ingredients such as nitrates and chlorates. 3. administration. 4. possession or use of the following prohibited and regulated drugs and precursor chemicals (See Table 4 in the Appendix) Since the department uses some of these compounds for laboratory classes and research. A separate “Permit to Purchase and Move Explosive and Explosive Ingredients” should also be obtained from PNP prior to purchase of nitrates and chlorates. transportation. Laboratory Operations & Safety Protocol 29 of 65 V1:2010 . Since these chemicals are also used in the teaching laboratories. Procedure for requesting for controlled chemicals by outside parties 1. sale. 2. the department chairperson approves the request. PDEA PDEA monitors and regulates the importation. The department chairperson together with the faculty-in-charge and/or the person in-charge of controlled chemicals decides whether to grant the request or not based on the amount of chemical on stock. transport and disposal. cyanide and their compounds. If the request is granted. transportation. The university is required to register its possession and use of Mercury and Cyanide compounds. the university is required to obtain a “License to Possess Explosive and Explosive Ingredients” from PNP.EMB The EMB regulates mercury. use. Requesting party proceeds to the Cashier to pay for the chemicals being purchased.

(c) a chemical that has a median lethal concentration (LC50) in air of 200 ppm but not more than 2. which is not corrosive. and o-toluidine. nitrosamines. Hepatotoxins: chemicals which produces liver damage. thiourea. agents which act on the hematopoietic system.000 ppm by volume or less of gas or vapor. It includes chemicals which are carcinogens. Systemic toxicity occurs when the chemical agent is absorbed into the bloodstream and distributed throughout the body. liver enlargement. Carcinogen: a chemical agent that causes a malignant disease or increases statistically the risk of cancer. hepatotoxins. and agents which damage the lungs. fume. or 2 mg/L or less of mist.Chapter 6 Handling Chemicals 6. Targeted Organ Effects a. toxic or highly toxic agents. Some of these compounds are fairly common materials used in many laboratories. reproductive toxins. skin. eyes. (b) a chemical that has a medial lethal dose (LD50) of 200 mg/kg but not more than 1.000 mg/kg body weight when administered by continuous contact for 24 hrs (or less if death occurs within 24 hrs) with the bare skin of albino rabbits weighing between 2-3 kg each. sensitizers. when administered by continuous inhalation for one hour (or less if death occurs within one hour) to albino rats weighing between 200-300 g each. 4. nephrotoxins. Sensitizer: a chemical that causes a substantial proportion of exposed people or animals to develop an allergic reaction in normal tissue after repeated exposure to the chemical. or dust. Irritant: a chemical. dioxane. or irreversible alterations in. (b) a chemical that has a medial lethal dose (LD50) of 200 mg/kg body weight when administered by continuous contact for 24 hrs (or less if death occurs within 24 hrs) with the bare skin of albino rabbits weighing between 2-3 kg each. Toxic: (a) a chemical that has a median lethal dose (LD50) of 50 mg/kg but not more than 500 mg/kg body weight when administered orally to albino rats weighing 200-300 g each. or 2 mg/L or less of mist. Corrosive: a chemical that causes visible destruction of. Toxic effects are also classified as acute or chronic. such as chloroform. 2. benzene. or mucous membranes. Signs & symptoms: jaundice. irritants. fume. but causes reversible inflammatory effect on living tissue by chemical action at the site of contact. Chronic effects result from long-term exposure or appear after a latency period. Hazardous chemical means any chemical which is capable of causing harm to people and the environment. 5. hydrazine. when administered by continuous inhalation for one hour (or less if death occurs within one hour) to albino rats weighing between 200-300 g each. Chemicals: carbon tetrachloride.1 Hazardous Chemicals All chemicals have toxic effects at some dose level for some route of exposure. corrosives. Local toxicity refers to the direct action of chemicals at the point of contact. whether by initiating or promoting it. carbon tetrachloride. (c) a chemical that has a median lethal concentration (LC50) in air of 200 ppm by volume or less of gas or vapor. Acute effects are observed shortly after exposure. Highly toxic: (a) a chemical that has a median lethal dose (LD50) of 50 mg/kg body weight when administered orally to albino rats weighing 200-300 g each. or dust. neurotoxins. 7. Chemicals can have local or systemic effects. benzene Laboratory Operations & Safety Protocol 30 of 65 V1:2010 . Health hazard means a chemical for which there is statistically significant evidence based on at least one study conducted in accordance with established scientific principles that acute or chronic health effects may occur in exposed personnel. 6. affecting one or more organs. 3. living tissue by chemical action at the site of contact. It is therefore wise to minimize exposure to chemicals. 1.

asbestos. 2. Compressed gas: (a) a gas or mixture of gases having. DBCP. or higher. absorption of moisture. proteinuria.1 oC. forms a range of flammable mixtures with air wider than 12% by volume. a compressed gas.8 oC or higher. but below 93. almost instantaneous release of pressure.4 oC. flammable: an aerosol that. Reproductive toxins: chemicals which affect the reproductive capabilities including chromosomal damage (mutations). an absolute pressure exceeding 40 psi at 21. Agents which damage the lung: chemicals which irritate or damage pulmonary tissue. in a container. e. 1. when tested by the method described in 16 CFR b. Signs & symptoms: conjunctivitis. or (b) a gas that. (b) a gas or mixture of gases having. Chemicals: carbon monoxide. and irritation. uranium. at an ambient temperature and pressure. when tested by the method described in 16 CFR 1500. Explosive: a chemical that causes sudden. 4.8 oC. tightness of chest. or retained heat from manufacturing or processing. g.3 oC. or high temperature. flammable: any liquid having a flashpoint below 37. c. an oxidizer. pressure. Cutaneous hazards: chemicals which affect the dermal layer of the body. Neurotoxins: chemicals which produce their primary toxic effect on the nervous system. and heat when subjected to sudden shock. loss of consciousness. flammable. and ASTM D 3278-78.3 oC. Chemicals: halogenated hydrocarbons. Signs & symptoms: narcosis.45. except any mixture having components with flashpoints of 93. decrease in motor functions. Chemicals: organic solvents. Physical hazard means a chemical for which there is scientifically valid evidence that it is a combustible liquid. cyanides. and effects on fetuses (teratogenesis). flammable: (a) a gas that. an organic peroxide. Signs & symptoms: cyanosis. ASTM D 93-79. the total volume of which make up 99% or more of the total volume of the mixture. Agents which act on the blood or hematopoietic system: decreases hemoglobin function. d. the total of which make up 99% or more of the total volume of the mixture. sterility. Chemicals: ketones. behavioral changes. Note: flashpoint means the minimum temperature at which a liquid gives off a vapor in sufficient concentration to ignite when tested according to ASTM D 56-79. shortness of breath. or a flashback (a flame extending back to the valve) at any degree of valve opening. unstable (reactive) or water-reactive. forms a flammable mixture with air at a concentration of 13% by volume or less. corneal damage. A chemical shall be considered to be a flammable solid if. Liquid. carbon disulfide. Solid flammable: a solid other than a blasting agent or explosive. chlorinated compounds. or (c) a liquid having a vapor pressure exceeding 40 psi at 37. 3. Combustible liquid: any liquid having a flashpoint at or above 37. an absolute pressure exceeding 104 psi at 54. or which can be ignited readily and when ignited burns vigorously and persistently as to create a serious hazard.8oC as determined by ASTM D-323-72. Chemicals: lead. pyrophoric. Chemicals: silica. c. explosive. h. regardless of the lower limit. Signs & symptoms: birth defects. regardless of the pressure at 21.8 oC. Nephrotoxins: chemicals which produce kidney damage. Aerosol. Eye hazards: chemicals which affect the eye or visual capacity. Laboratory Operations & Safety Protocol 31 of 65 V1:2010 . Chemicals: mercury. deprive the body tissues of oxygen. Signs & symptoms: defatting of the skin. f. spontaneous chemical change. that is liable to cause fire through friction. Signs & symptoms: cough. Gas. except any mixture having components with flashpoints of 37. d.2 oC. gas. in a container. at ambient pressure and temperature. Signs and symptoms: edema. Flammable: a chemical that falls into the following categories: a.b. yields a flame projection exceeding 18 inches at full valve opening. rashes. acids.

For many operations it is not obvious that an aerosol is being generated and laboratory personnel may not be aware that a hazardous situation exist. Inhalation hazards are often associated with gases and volatile chemicals. injections with a needle and syringe. 5. sores. by local action. 7. pouring. The main portals of entry for chemicals through the skin are the hair follicles. Actually. Oxidizer: a chemical other than a blasting agent or explosive. or will become self-reactive under conditions of shocks. its concentration. or as produced or transported. and cuts or abrasions of the outer layers of the skin. Water reactive: a chemical that reacts with water to release a gas that is either flammable or presents a health hazard. mists. pressure or temperature. and removing caps and stoppers. all laboratory operations involving an open vessel will result in the release of an aerosol. Many of the particulates generated during such procedures do not settle out but remain suspended in the air and are carried about by air currents in the room. Inhaled gases or vapors may pass rapidly into the capillaries of the lungs and be carried into the circulatory system. Alert laboratory personnel will take care not to create unnecessary aerosols. glassware. Laboratory chemicals in the form of dusts and particulates can become airborne when transferred from one container to another. Such operations include weighing. Inhalation of toxic vapors. Some of these particulates are capable of being inhaled and deposited in the respiratory tract.1500. where one or both of the hydrogen atoms has been replaced by an organic radical. Grinding and crushing procedures can also produce aerosols. that initiates or promotes combustion in other materials. throat. or equipment which become contaminated curing experimental activity. decompose. 9. or cuts. and the duration of exposure. The degree of injury resulting from inhalation of toxic substances depends on the toxicity of the material. Spills and splash can result in overt contamination of the skin. Chemicals can also gain entrance into the body when contaminated hands touch the mouth. condense. and lungs and can seriously damage these tissues. and which may be considered to be a structural derivative of hydrogen peroxide. Splash created from spills and during vigorous shaking and mixing also results in aerosol formation. However. One of the most frequent exposure to chemicals is by contact with the skin. pipeting. 6. a number of materials are absorbed through the skin to produce systemic poisoning. stirring. sebaceous glands. which facilitate the absorption of chemicals into the body. 8. will vigorously polymerize. thereby causing fire either of itself or through the release of oxygen of other gases. Also. it ignites and burns with a self-sustained flame at a rate greater than one-tenth of an inch per second along its major axis. sweat glands. or dusts can produce poisoning by absorption through the mucous membrane of the mouth. its solubility in tissue fluids. Unstable (reactive): a chemical which in the pure state. 1. but solids and non-volatile liquids can also present an inhalation hazard for laboratory personnel. Inhalation. 3. laboratory personnel may unconsciously contaminate themselves when they touch work surfaces. eyes.2 Routes of Exposure Dermal Contact. nose. The follicles and glands are supplied with blood vessels.44.4 oC or below. Pyrophoric: a chemical that will ignite spontaneously in air at a temperature of 54. A common result of skin contact is localized irritation or dermatitis. Laboratory Operations & Safety Protocol 32 of 65 V1:2010 . 6. Organic peroxide: an organic compound that contains the bivalent —O-O— structure. 2. gases.

nose bridge width. Food items and utensils themselves can become contaminated when stored in the laboratory. Contact Lenses: Contact lenses do not provide eye protection in the industrial sense. A face shield or mask may be needed when a vacuum system (which may implode) is used or when a reaction that has a potential for mild explosions is conducted. The needle and syringe is one of the most hazardous items used in the laboratory. Ocular Exposure. Safety glasses: Ordinary prescription glasses do not provide adequate protection from injury to the eyes. vapor. The minimum acceptable eye protection requires the use of hardened-glass or plastic safety spectacles.g. containers of toxic chemicals may break resulting in hazard from contact with broken contaminated glass. These should have: a minimum thickness of 3 mm. and must be worn only in conjunction with approved safety eyewear. The American Chemical Society’s Committee on Chemical Safety states in Safety in Academic Chemistry Laboratories that. welding. There are specific goggles and masks for glassblowing. The eyes are of particular concern because they are so sensitive to irritants. staff. For full protection. Splash goggles (“acid goggles”) that have splash –proof sides should be used when protection from harmful chemical splash is needed. or splashes.Ingestion. 3. and intense light sources. Full-face shields that protect the face and throat should always be worn when maximum protection from flying particles and harmful liquids is needed.3 Protective Clothing and Equipment Eye and Face Protection. more than two hours per day). Goggles: Goggles are not intended for general use. “Contact lenses should not be worn in the chemistry laboratory Laboratory Operations & Safety Protocol 33 of 65 V1:2010 . They are intended for wear when there is danger of splashing chemicals or flying particles. and lens diameter. intense heat. Safety spectacles are recommended for those who require eye protection frequently and/or for long durations (e. Other eye protection should be worn when a significant splash hazard exists. and visitors in laboratories where chemicals are stored or handled. goggles should be worn when working with glassware under reduced or elevated pressure and when glass apparatus is used in combustion or other high-temperature operations. 4. Three dimensions that are important in providing a comfortable fit include: temple length. such as lasers and ultraviolet light. 1. In addition. and lens-retaining frames. Impact-protection goggles have screened areas on the sides to provide ventilation and reduce fogging of the lens and do not offer full protection against chemical splashes. Ocular exposure can occur via splash or when contaminated hands rub the eyes. It is the responsibility of the lab supervisor to determine the level of eye protection required and to enforce eye-protection rules. or highly particulate atmosphere. Eye and protective devices must be worn by students. Ingestion of toxic materials in the laboratory can also occur when contaminated hands come in contact with the mouth of with food items which are placed in the mouth. Also. For example. identification of contact lens wearers should be ensured for appropriate emergency care and for protection in work areas hazardous to the eyes. Accidents involving needles and syringes can results in injection contamination through the skin. The type of safety device required will depend on the nature of the hazard and the frequency with which it is encountered. contact lens use should be restricted. when the work environment entails exposure to chemical fumes. Injection. The practice of mouth pipeting can result in aspiration of toxic materials. safety glasses should be worn with face shields. impact resistance. 6. Furthermore. molten metals. Few substances are innocuous in contact with the eyes and a considerable number are capable of causing burns and provide for rapid absorption of many chemicals. Side shields that attach to regular safety spectacles offer some protection from objects that approach from the side but do not provide adequate protection from splashes. Face Shields: Goggles offer little protection to the face and neck. 2. passed flammability tests. faculty.

nitrile. Use of Gloves. Leather gloves may be used for handling broken glassware. and for similar operations where protection from chemicals is not needed. and may itself present a hazard (e. Laboratory Operations & Safety Protocol 34 of 65 V1:2010 . thickness and permeation rate and time) are known. belts) can be especially hazardous because many chemicals can be absorbed in the leather and then held close to the skin for long periods. plastic aprons can accumulate considerable static charge and should be avoided in areas where flammable solvents could be ignited. Contact lenses can also trap particulate foreign matter in the eye and thereby produce abrasion of the cornea. personal clothing that will be worn home should be covered by protective apparel. Skimpy clothing offers little protection to the skin in the event of chemical splash. only contact lenses worn for therapeutic reasons can be permitted in the laboratory. Coats and aprons do not significantly resist penetration by organic liquids and. Double gloving is recommended when handling highly toxic or carcinogenic materials. Such personnel should not wear loose. Before removal. especially any that has become contaminated. Various synthetic materials such as Nomex and Kevlar can be used briefly up to 538 oC. he is warned against absorption through the skin of such substances as HCN gas. However. gloves should be washed. a known carcinogen. and their suitability for the operation being conducted. or become ensnarled in apparatus and moving machinery. Proper gloves should be worn whenever the potential for contact with corrosive or toxic materials and materials of unknown toxicity. aniline vapor. Cotton (pure or 60:40 cotton-polyester). It is the responsibility of the lab supervisor to determine whether specialized hand protection is needed for any operation and to ensure that needed protection is available. the particular hazard involved. and synthetic materials such as Nomex or Tyvek are satisfactory. punctures. Loose or torn clothing and unrestrained long hair can easily catch fire. or torn clothing and unrestrained long hair. should be removed immediately. It is best not to use gloves made either entirely or partly of asbestos. sharp edged objects. but they are by no means total respiratory protection as air masks. although the wearer is protected against inhalation exposure. Insulated gloves should be used when working at temperature extremes.e. Laboratory coats and aprons are intended to prevent contact with dirt and the minor chemical splashes or spills encountered in lab-scale work.except for therapeutic reasons… In the event of a chemical splash into an eye. Respiratory Protection. Furthermore. Gloves should be selected on the basis of the material being handled. For these reasons. and tears. Common glove materials include neoprene. shoes. Lab workers should know the appropriate techniques for removing protective apparel. the removal of the contact lens to achieve immediate irrigation is made nearly impossible by involuntary spasm of the eyelid… Gases and vapors can concentrate under the lenses and cause permanent eye damage. rayon and polyesters are not. If the possibility of chemical contamination exists. dip into chemicals. These. combustibility) to the wearer. These materials differ in resistance to various substances. In that event. Before each use. Finger rings can react with chemicals and also should be avoided around equipment that has moving parts. Glove materials are eventually permeated by chemicals. etc. are primarily a protection for clothing. Plastic or rubber aprons provide better protection from corrosive or irritating liquids but can complicate injuries in the event of fire. Clothing and Protective Apparel. Chemical spills on leather clothing or accessories (watchbands. Such items must be removed promptly and decontaminated or discarded. skimpy. if significantly contaminated by them.g. if the material is impermeable to water. and butyl and natural rubbers. which are self-contained breathing apparatuses with their own air tanks. for inserting glass tubes into rubber stoppers. capillary action tends to hold the offending liquid under the contact lens and against the surface of the cornea.” Masks may be used to minimize exposure to noxious fumes. however. polyvinyl chloride. and very hot or very cold materials. The clothing worn by lab workers can be important to their safety. Furthermore. they can be used safely for limited time periods if specific use and glove characteristics (i. gloves should be inspected for discoloration.

Dry sweeping or dry mopping contaminated laboratory floors could re-aerosolize contamination. Placing a reacting chemical system within a hood. These should be placed in properly-labeled waste containers for disposal. The laboratory door should be kept closed while experiments are in progress. 3. Care should also be taken when discarding contaminated cloves or plastic backed absorbent paper used to cover the work surface so that contamination is not aerosolized in the process. Laboratory Operations & Safety Protocol 35 of 65 V1:2010 . Minimizing Aerosols. drinking.Foot Protection. Perforated shoes. food. Chemical fume hoods are the primary containment device in the laboratory to control airborne contaminants generated during experimental procedures. sprays. but also reduces interruptions to the lab worker which could lead to accidents. fires. Shoes should be worn at all times in laboratories or other areas where chemicals are used or stored. etc. application of cosmetics. chewing of gum. or moving. Chemical fume hoods provide personnel protection by means of directional airflow from the laboratory into the hood through the face opening. especially with the hood sash closed. This airflow reduces the potential for escape of airborne contaminants into the laboratory. 4. smoking. Safety shoes are required for personnel whose job duties require the lifting. Safety shoes are used to protect the feet against injuries from heavy falling objects. Personnel should wash their hands immediately after completion of any procedure involving toxic chemicals and when they leave the lab. carrying.4 Handling Toxic Materials Access Control. or against lacerations from sharp edges. Procedures involving volatile chemicals and those involving solids or liquids that may result in the generation of toxic aerosols should be conducted in a chemical fume hood rather than on the open bench. or cloth sneakers should not be worn in labs or areas where mechanical work is being done. or storage of utensils. Rapid mixing of liquids with pipets by alternate suction and expulsion or forcibly expelling material from a pipet should be avoided. or food containers should not be allowed in labs where toxic materials are used. Floors should be cleaned with a wet mop or with a vacuum cleaner equipped with a HEPA filter. Dropping the contents from a height generates greater aerosol. Personnel Practices. of objects weighing more than 15 lbs. Access to laboratories which have toxic chemicals should be controlled. or allowing the contents to run down the wall of the receiving vessel. Use of Chemical Fume Hoods. if dropped. Discharge fluids from pipets as close as possible to the fluid level of the receiving vessel. 6. against crushing by rolling objects. This not only protects those people who might otherwise enter the lab. Decontamination of Work Surfaces. Since all procedures involving an open vessel of liquids or powders generate aerosols. which. The practice of mouth pipeting should also not be allowed. Other items and equipment which become contaminated during experimental activity should be decontaminated with the appropriate solvent. places a physical barrier between the workers in the lab and the chemical reaction. the laboratory worker should develop techniques which will minimize the creation of aerosols: 1. sandals. This barrier can afford workers protection from chemical splash. Eating. would likely result in a foot or toe injury. The use of liquid soap dispensers is recommended. 2. Mechanical pipeting aids are to be used for all pipeting procedures. Work surfaces should be protected from contamination by using disposable plastic backed absorbent paper or stainless steel trays. and minor explosions.

Protection of Vacuum Lines. or be removed for subsequent disposal. Contaminated materials must either be decontaminated by procedures that decompose the chemical carcinogen. are to be placed entirely within a chemical fume hood. it should be placed in a tightly-stoppered sample tube or otherwise safeguarded from contaminating the lab. An inventory of stock quantities should be maintained.The following practices should be observed when using fume hoods: 1. when used with chemical carcinogens.5 Handling Carcinogens Procedures for handling toxic materials also applies to chemical carcinogens. Quantities of chemical carcinogens present in the work area should be kept to a minimum. vapors or aerosols produced by these instruments should be captured through local exhaust ventilation at the site of their production. Check efficiency of the hood by measuring its average face velocity regularly. a separate vacuum pump or other device placed in an appropriate chemical fume hood should be used. Storage vessels containing working quantities should be labeled: CAUTION – CHEMICAL CARCINOGEN. Analytical equipment that becomes contaminated should not be used until it has been completely decontaminated. The outer container is to be labeled both the name of the chemical carcinogen and the warning: CAUTION – CHEMICAL CARCINOGEN. Laboratory Transport. 3. Verify airflow PRIOR to doing an experiment by attaching a Kimwipe or ribbon to the sash. When this is impossible. Storage. is to be protected with an absorbent or liquid trap and a HEPA filter to prevent entry of any chemical carcinogen into the system. 7. 2. The procedures outlined in this section are additional precautions in dealing with carcinogenic materials. and Identification. 6. Contaminated materials which are transferred from work areas to disposal areas must first be placed in a closed plastic bag or other suitable impermeable and sealed primary container. 8. 6. Use of Analytical Instrumentation. Hood work areas should be clear of unnecessary equipment and materials which can disrupt airflow and block vents. Chemical carcinogens Laboratory Operations & Safety Protocol 36 of 65 V1:2010 . 5. and their access controlled. When a sample is removed from the analytical instrument. Face velocities between 100 to 120 feet per minute are acceptable for most uses. Experiments should be planned so that. Working Quantities. When using a volatile carcinogen. Storage vessels containing stock quantities should be labeled: CAUTION – CHEMICAL CARCINOGEN. all of the materials needed for a procedure are present in the hood to eliminate disruption of airflow by carrying equipment in and out during a procedure. The primary container must be placed in a durable outer container before being transported. Keep sash as low as possible. as much as possible. Stock quantities of chemical carcinogens are to be stored in designated storage areas. Decontamination. including water aspirators. Storage vessels containing chemical carcinogens are to be first placed in an unbreakable outer container before being transported to lab work areas. Inventory. Quantities should not normally exceed the amounts required for use in one week. Disruptive room air currents should be minimized by avoiding traffic near fume hoods and opening and closing doors near fume hoods while experiments are in progress. Analytical instruments. The storage areas should be posted with signs bearing the legend: CAUTION – CHEMICAL CARCINOGEN Authorized Persons Only. Hoods should not be used for storage of chemicals. Work should be carried out as far back in the hood as possible. and should include the dates of acquisition and disposition. Use equipment with legs. 4. Each vacuum service.

and burn or to explode flammable materials varies with the specific type of substance. oil baths. dust accumulations. close attention should be given to all potential sources of ignition in the vicinity. A means for assuring adequacy of clean-up should be provided. The basic precautions for safe handling of flammable materials include the following: 1. especially when materials are stored or disposed of. 3. peroxides. The rate at which different liquids produce flammable vapors depends on their vapor pressure. Handling. Flammable substance should be handled only in areas free of ignition sources. organic materials mixed with strong oxidizing agents (such as nitric acid. 2. For example: A steam line or a glowing light bulb may ignite carbon disulfide (ignition temperature 80 oC). Many common laboratory solvents and chemicals have flashpoints that are lower than room temperature. ignite. 3. ASTM D 93-79. When transferring flammable liquids in metal equipment. Materials susceptible to spontaneous combustion include oily rags. and ASTM D 3278-78. When flammable materials are used in the lab. 1. the bonding can be made to the liquid rather than to the container. alkali metals such as sodium and potassium. 6. Flammable substances should never be heated by using an open flame. it is the vapors from the liquid that burn. If the path of vapor with the flammable range is continuous. The possibility of spontaneous combustion should be considered. and hot surfaces. The degree of the fire hazard also depends on the ability to form combustible or explosive mixtures with air. Diethyl ether (ignition temperature 160 oC) can be ignited by the surface of a hot plate. permanganates. chlorates.which have spilled out of a primary container so as to constitute a hazard must be inactivated in situ or should be absorbed by appropriate means for subsequent disposal. The vapors of flammable liquids are heavier than air and capable of traveling considerable distances. Ignition Temperature (Auto-ignition temperature): the minimum temperature required to initiate or cause self-sustained combustion independent of the heat source. Flammable vapors from massive sources such as spillages have been known to descend into stairwells and ignite on a lower story. heating mantles. Metal lines and vessels discharging flammable substances should be properly bonded and grounded to discharge static electricity. and phosphorous. or heat in labs which can ignite flammable substances: open flames. flame. static electricity. This possibility should be recognized. Prevention of fires and explosions requires knowledge of the flammability characteristics (limits of flammability. A flammable liquid does not itself burn. Flashpoint: the minimum temperature at which a liquid gives off a vapor in sufficient concentration to ignite when tested according to ASTM D 56-79. Preferred head sources include steam baths. When nonmetallic containers (plastic) are used. There are a number of potential sources of spark. and burning rates) of combustible materials likely to be encountered under various conditions of use. and special note should be taken of ignition sources lower than that at which the substance is being used. for instance wipe tests or fluorescence tests. Properties of Flammable Substances. the flame will propagate itself from the point of ignition back to its source. and persulfates). 2. This is of special concern when working in a dry environment such as an air-conditioned room or a room with a dehumidifier. water baths. ignition requirements. finely divided pyrophoric metals. lighted matches. Sources of Ignition. and hot air baths. and of the appropriate procedures top use in handling such substances. static-generated sparks should be avoided by bonding and use of ground straps.6 Flammable Materials The ability to vaporize. Spontaneous Ignition or Combustion: this takes place when a substance reaches its ignition temperature without the application of external heat. Laboratory Operations & Safety Protocol 37 of 65 V1:2010 .

heated in open containers. or when a drum is filled. Metals: Combustible metals such as magnesium and sodium require special extinguishers labeled D. offer first defense against flammable liquids or electrical fires without leaving a powder residue which could harm electronic equipment. Storage Rules 1. energized electrical equipment and other electrical sources require a dry chemical. formation of flammable gases. “Gas” extinguishers. 6. allowed to stand in open containers. solubilization of toxic substances. Laboratory Operations & Safety Protocol 38 of 65 V1:2010 . They may result when incompatible chemicals are accidentally spilled. labels A. Containers of flammable liquids shall not be drawn from or filled within buildings unless provision is made to prevent the accumulation of flammable vapors in hazardous concentrations. light. wood. Flammable liquids must be stored so that accidental contact with strong oxidizing agents (such as permanganates or chlorates) is avoided. 2. 5. 4. or when they are unwittingly combined during experimental procedures. and. more recently.7 Handling Explosive/Reactive Materials The large number of different classes of chemicals potentially present in a research laboratory poses increased risks from accidental hazardous chemical reactions or explosions. Halon or CO2 extinguisher labeled B. Halon. 3. volatilization of toxic or flammable substances. Hazardous reactions may cause any one or more of the following: heat generation. dispersal of toxic dusts/mists/particles. both the drum and other equipment must be individually electrically grounded and bonded to each other. Flammable liquids must not be stored in domestic type refrigerators. formation of toxic vapors. Keep flammable liquids away from heat and direct sunlight.4. 2. Use refrigerators that have electrical contacts (door switch. Fire extinguishers are labeled according to their fire fighting proficiency and safety in extinguishing various types of fires. thermostat) removed or exteriorized. 5. Flammable Liquids: Fires in solvents and other flammables liquids require dry chemical. C. pictograms are used to indicate the type of fire on which an extinguisher is to be used. Ordinary Combustibles: Fires in paper. When a flammable liquid is withdrawn from a drum. or D. explosion. Flammable liquids must not block lab aisles or exits. or handled in any other way. formation of shock or friction sensitive compounds. rubber. For easy identification. fuse boxes. Fire Extinguishers. A hazardous reaction occurs when two or more incompatible chemicals result in an undesirable or uncontrolled reaction with adverse consequences. B. cloth. An exhaust hood should be used whenever appreciable quantities of flammable substances are transferred from one container to another. when they are inadvertently mixed as chemical waste. Most chemical laboratory fire hazards require dry chemical multipurpose extinguishers (ABC) which must be installed in hallways. Flammables stored in the open in the laboratory work area shall be kept to the minimum necessary for the work being done. These are identified accordingly: 1. and many plastics require water or dry chemical type extinguisher labeled A. pressurization in closed vessels. Using the “wrong” type of extinguisher on a fire can endanger the user and make the fire worse. and violent polymerization. 4. formation of substances of greater toxicity. 6. containing Halon 1211 or CO2. Ventilation is one of the most effective ways to prevent the formation of flammable mixtures. or CO2 extinguisher labeled C. 3. fire. Electrical Equipment: Fires in wiring.

the disaster is compounded by the adverse reaction. Laboratory Operations & Safety Protocol 39 of 65 V1:2010 .It is easy to fall into the trap of becoming complacent with certain chemicals used everyday with certain procedures. If there is an accident. Incompatibility of chemicals is the prime reason for not storing chemicals on the shelf alphabetically. It is prudent to check for incompatibility wherever a change is made in chemical procedures.

oxidizers. 4. Corrosivity. Reactivity. capable of detonation or explosive reaction. Recyclable materials should be kept separate from other waste. Toxicity. Non-Halogenated Flammable Solvents. solids capable of burning vigorously and persistently after ignition through friction. Ignitability. diluted to non-flammable concentrations. but followed by flushing with copious volumes of water. toxic.g. detoxify. e. Laboratory Operations & Safety Protocol 40 of 65 V1:2010 . reacts violently with water. Efforts should be made to decontaminate. less than oral LD50 of 50 mg/kg (rats). Oils. mutagenic. Only trace quantities of oils associated with cleaning and washing operations should be released to the sanitary sewer. or suspected carcinogenic.5. forms potentially explosive mixtures with water of pH conditions between 2 and 12. Acids and Bases. 3.Chapter 7 Waste Disposal 7. Liquids with a flashpoint less than 60 oC. there must be careful consideration about which wastes can be combined. teratogenic. 7. These should be converted to sulfide. 7. Fatal to humans in low doses. the more economical is the disposal method for that waste. Normally unstable and undergoes violent changes. and carbon tetrachloride. inhalation LC50 of 2 mg/L (rats). If this is not practical. Halogenated solvents must not be combined with flammable nonhalogenated solvents. A waste stream generated from a laboratory procedure should not be combined with other chemical wastes. carcinogenic. chloroform. Examples of halogenated solvents include methylene chloride. Heavy Metals. The fewer the number of chemicals associated with a waste. sodium metal.1 Waste Characteristics Hazardous wastes are classified according the following: 1. which is less soluble. Aqueous solutions with a pH less than or equal to 2. before final disposal. or greater than or equal to 12.5. or otherwise render the waste nonhazardous.3 Handling Specific Wastes Individual Waste Streams. 2. or spontaneous chemical changes at standard temperature and pressure. Non-halogenated flammable solvents may be sent to the incinerator and must be free of heavy metals and reactive materials. liquids which corrode steel at a rate greater than 6. Small volumes of acids and bases can be diluted and discharged in the sanitary sewer. Disposal of solvents to the sanitary sewer is limited to low-toxicity solvents.35 mm per year at 55 oC. absorption of moisture. Different waste materials should be segregated whenever possible. neutralize.2 Waste Reduction Minimizing chemical waste generation also minimizes safety hazards. It is encouraged that laboratories consider ways of reducing the volume of waste or preserving the reuse of the materials through the redesign of experiments. Halogenated Solvents. or dermal LD50 of 200 mg/kg (rabbit). Acids and bases containing heavy metals must not be disposed to the sewer system. miscible in water.

All hazardous characteristics of waste should be identified. 4. heat generation. Each compound in the waste containers must be listed by its complete chemical name. capillary tubes and scalpels should be placed in puncture-resistant waste collection containers prior to disposal. Do not use abbreviations. mutegenicity. Provide the following information: • Name • Chemical waste classification • Chemical wastes to be kept in the bottle • Name of mentor 3. If the waste is an acid or a base. Filled-up waste bottles should be submitted to the stockroom for signing of clearance at the end of the school year Safety Carriers. 7. and solid waste in pounds or kilograms. to caution the handler. Gallon-sized four-liter glass reagent bottles are generally the most convenient. 7. and the approximate percentage of each compound. should be noted. 2. Waste Characteristics. Materials that may create a fire or explosive hazard.5 Waste Containers Containers. Glass collection containers must preferably be stored in rubber safety carriers to protect against breakage and spillage. razor blades. or teratogenicity should be specified beside “Toxic”. Corrosive materials with pH less than 5. 4. If metal or plastic containers are used. etc. fire. Obtain waste bottles for chemical wastes from the stockroom 2. Any toxic or reactive hazards.Sharps. Liquid waste is to be given in gallons or liters. Amount. 2. Principal Constituents. 3/4 full. Special Handling Considerations.4 Labeling Each container must be labeled with the following information: 1. giving the container size and indicating whether it is full. Procedure for obtaining chemical waste bottles 1. The amount of waste. Substances or conditions which could result in an explosion.6 Disposal to Sewerage System Avoid discharges to the sanitary sewer of the following: 1. Laboratory Operations & Safety Protocol 41 of 65 V1:2010 . Carcinogenicity. be sure that the container material is compatible with the waste. pasteur pipettes. A bottle with a barcoded label will be issued by the stockroom personnel to the student. the approximate pH of the waste should be indicated beside “Corrosive” on the label. 7. 3. or flammable gas generation should be explained. The terms most appropriately describing the waste should be included. Sharps. including but not limited to syringes.

K. Mn. Ca. Non-hazardous wastes may be disposed of as ordinary trash. K. Mg. nucleic acids. Ca. Si. Co. or contamination of sludge of effluent from the wastewater treatment. Ca. Solid or viscous materials in amounts to obstruct flow or interfere with operations. Mg. Mg. K. 5. NH4 • Carbonates: Na.3. the following nonhazardous materials may be placed in ordinary trash bins for disposal: Agarose Alumina Aluminum oxide Ammonium phosphate Calcium carbonate Calcium oxide Calcium phosphate Calcium sulfate Citric acid Dextrin Glycine Magnesium carbonate Magnesium chloride Magnesium sulfate Potassium carbonate Potassium chloride Potassium sulfate Sephadex Silica gel Sodium chloride Sodium citrate Sodium phosphate Sodium sulfate Stannous oxide Starch Sugars Titanium oxide Materials listed below in quantities up to about 100g or 100 mL at a time are suitable for disposal down the drain while flushing with excess water Organic Chemicals • Alkanols with fewer than 4 carbons • Aliphatic aldehydes with fewer than 5 carbons • Amides with fewer than 5 carbons • Aliphatic amines with fewer than 7 carbons • Carboxylic acids with fewer than 6 carbons • Esters with fewer than 5 carbons • Proteins. In general. Mg. NH4 • Oxides: Mg. Cu • Chlorides: Ca. Heat discharges which will inhibit biological activities or increase the waste water treatment effluent about 40 oC. Non-hazardous wastes in liquid form may be disposed of down the drain.7 Disposal of Chemical Wastes Non-hazardous Chemical Wastes 1.” 2. Discharges of any toxic material in volume of strength to cause interference with waste treatment processes. NH4 • Borates: Na. However. Ca Laboratory Operations & Safety Protocol 42 of 65 V1:2010 . Ti. Sr. soluble fats and their precursors Inorganic Chemicals • Sulfates: Na. Al. carbohydrates. Sr. K. 4. Na. 7. the containers should be labeled and marked as “Non-hazardous. Sr. Fe.

Hazardous chemical wastes Hazardous chemical wastes should be placed and segregated into leak-proof barcoded waste bottles following the classification of the DENR-EMB (See Table 5 in the Appendix). Laboratory Operations & Safety Protocol 43 of 65 V1:2010 . These wastes will be hauled by DENR-accredited transporters for treatment and disposal.

Always wash hands after removing gloves and before leaving the lab. There are designated refrigerators (Biochemistry and Room 103 NCIC labs) for short-term storage (2 months or less) and a cryogenic container (Rm 103 NCIC lab) for long-term stock storage. First aid kit b. 2. 5. Do not pipette by mouth. 6. there are four levels as specified by US Department of Health and Human Services and National Institutes of Health. fungi. and Microbiology Safety and Staff Induction Manual. Before using any of these samples. know the biosafety level (BSL) of the organism first. a. Always wear mask and put on gloves in handling samples. mask. Food is stored outside the work area in cabinets or refrigerators designated specifically for that purpose. Samples must be properly labeled to immediately identify the organism present. Remove and change gloves when overtly contaminated or when torn or punctured. and yeast. Microorganisms refer to organisms of microscopic or sub-microscopic sizes such as bacteria.Fire extinguisher/blanket Laboratory Operations & Safety Protocol 44 of 65 V1:2010 . (Refer to Table 6 of the Appendix) 3. Store the samples in these designated places only to avoid spreading and contamination of bio-free chemicals and solutions.1 General Information Most of the procedures are adapted from Environmental Health and Safety. spray a solution of 70% alcohol (usually ethanol) on the gloves to disinfect. From time to time. University of Wyoming. Only organisms under BSL 1 are allowed in the laboratory.2 Storage 1. remove and leave lab apron or coat in the lab. The secondary container should be sealable and non breakable. 2.3 Transport When transporting samples to or from the laboratory. Persons wearing contact lenses should also wear eye protection. Princeton University. Do not wear contaminated gloves outside the lab.4 Handling 1.Chapter 8 Biological Samples: Handling and Waste Disposal 8. 7. the "primary" container should be carried within an outer "secondary" container. 8. Lab apron must be worn at all times. 8. 4. When leaving the lab. Do not wash or reuse disposable gloves. The lab apron or coat must be laundered every two weeks. University of Tasmania . Familiarize yourself with the location and operation of the following emergency items for each laboratory. face shield or other splatter guard) for anticipated splashes or sprays of infectious materials to the face when agents must be handled outside the BSC (biosafety cabinet). Wear appropriate face protection (goggles. virus. 3. only mechanical pipetting devices are permitted. For bacteria specimens. 2. 8.Launceston Campus 1.

5. To prevent accidents. One pressure cooker is designated for sterilization purposes while another is solely used for decontamination. Consider the materials necessary for the planned work in the cabinet. This applies particularly to the flaming of the bacteriological loop: the loop should be drawn gradually from the cooler to the hotter parts of the flame. 6. if the cabinet is not left running. mask. Leave for 10 minutes and then mop up. Always use heat safety gloves when removing items from the pressure cooker. Working in a BSC (adapted from Environmental Health and Safety. 10. put on gloves. The lid should only be open when the pressure is zero. working with materials from the clean to the dirty side. time and contents of the cycle. The working area should be wiped with disinfectant at the beginning and end of the laboratory session. A laminar flow cabinet is also available in the Biochemistry lab. Before using the equipment. Always wear safety glasses when opening the pressure cooker. 2. 4. Place items into the cabinet so that they can be worked with efficiently without unnecessary disruption of the air flow. and gloves. cover spill with paper towel and pour on disinfectant. Adjust the working height of the stool so that the worker's face is above the front opening. Exits 8. 3.Eyewash station e.c. In the logbooks of either pressure cooker. A heat sensitive indicator (autoclave tape) should be used in every load. Always wash your hands before leaving the laboratory. The standard autoclave cycle used in this laboratory is 15 psi for 15 minutes. 9. Work at a moderate pace to prevent the air flow disruption that occurs with rapid movements. At a minimum. 3. For minor spills. 2. Delay manipulation of materials for approximately one minute after placing the hands/arms inside the cabinet. Do not disturb the airflow by covering any of the grillwork with materials. wait until the pressure drops to ~5 psi before opening the exhaust valve. environmental. It is housed in the isolation room in C305. No slides or cultures are to be taken from. Turn the cabinet on for at least 10 . this will include a laboratory apron/coat. Carry out procedures so as to minimize the risks of spills. Wear appropriate personal protective equipment. and sample/product protection. 11. Laminar Flow Cabinet/Biosafety Cabinet (BSC) 1. or brought into the laboratory without permission of the instructor or laboratory in-charge. 9. 10. splashes and the production of aerosols. Wipe the bottom and side of the hood surfaces with disinfectant when work is completed. 6. Princeton University) 1. Laboratory Operations & Safety Protocol 45 of 65 V1:2010 . 4. 11. 8. make sure that the door of the isolation room is closed to minimize the disruption of the airflow. Gas isolation switch d. 12. Minimize the frequency of moving hands in and out of the cabinet. If you have an accident of any kind. Disinfect work surface with 70% alcohol or other suitable disinfectant. The BSC in the laboratory is a Class 2 cabinet designed to provide personnel. 2. 8. indicate the date. 5. call the instructor immediately. 7.15 minutes prior to use.5 Laboratory Equipment Autoclave 1.

3. break. The materials must be decontaminated immediately. preferably made of glass or other autoclave-compatible materials. Liquid Decontamination • • • Add liquid chlorine bleach to provide a final 1:10 dilution Let stand at least 20 minutes Discard down the drain Surface Decontamination • • Wipe with 1:10 dilution of chlorine bleach. consummable items. tongs. After the microbial laboratory activity. For example. The container must be covered by aluminum foil. (adapted from Environmental Health and Safety. the following steps must be considered. and water or as thorough as sterilization. Dispose of needles and syringes in the puncture resistant container provided in the laboratory for this purpose. within the day or the day after. A container for used items (pipet tips.6 Waste Disposal 1. The standard decontamination cycle used in this laboratory is 15 psi for 15 minutes. culture loops. put the glassware and other materials directly touched by culture media or contaminated with microorganism in a box or leave them inside the BSC. For example. In the event of spill incidents. 4. 2. how pathogenic are the organisms contained or likely to be contained in the spill. shear. if possible. After decontamination. liquid wastes are thrown down the drain while solid wastes are thrown in trash bins. All glassware. Place full containers in an autoclave bag and sterilize before disposal in waste boxes. others) must be prepared. and is infection likely to be acquired by the respiratory route? The physical nature of the spill. has the spill resulted from a container knocked over on a bench with low potential for the generation of aerosols. Sterilization is done using the pressure cooker designated for decontamination purposes. 2. Do not bend. 8.7 Clean-up Procedure 1. or has a container been dropped. Clean the working area using surface decontamination procedure. and other materials used in the microbial activity must be decontaminated. Princeton University) Assessing a Biohazard Spill The biological nature of the spill. or forceps – not directly with hands. Handle broken glassware with brush and dustpan. pipet. A decontamination procedure can be as simple as clean-up with a solution of detergent. 2. bleach. recap. or smashed in some way with a greater potential for the generation of aerosols? Laboratory Operations & Safety Protocol 46 of 65 V1:2010 .8. or remove used needles from disposable syringes or otherwise manipulate such units by hand before disposal. 3. or Wipe with 70% alcohol 1.

put an absorbent material over the fluid. Treat all body fluids as if they are infectious. If hands have been contaminated. Remove gloves and wash hands. Use disinfectant to wipe over areas around the spill that are likely to have been contaminated with splashes and aerosols. 3. Minor biohazard spills Generally considered as a spill of minimally hazardous material with low potential for generation of aerosols. 1. 8. semen. 2. 10 ml broth culture of E. warn others of spill and all must leave the room immediately. Don’t forget to wear gloves. vaginal secretions. Dispose the gloves by sealing them in a plastic bag and placing it in a trash bin. 4. Put on gloves. 1. and oral or nasal secretions. first wash hands with strong detergent followed by generous spray of 70% alcohol. Clean and disinfect the area with freshly prepared 1:10 (bleach: hot water) solution. Always wear gloves in dealing with body fluids. gloves. Wash the hands thoroughly with hot water and soap. Close doors and place a "DO NOT ENTER" sign on the door. Lay paper towels wetted with disinfectant onto the spill and leave for 30 minutes. 7. Major biohazard spills Generally considered to be spills of major risk with larger volume and considerable production of splashes and aerosols. 8. For example. Notify laboratory in-charge(preferably) or other faculty member The clean up team should all don "gowns. Determine the extent of the spill: pour disinfectant around the edge of the spill and allow it to run "into the spill". vomit. 2. 2. 9. 3. 7. Do not re-enter the room until a minimum of 30 minutes has elapsed. Lay down absorbent material wetted with disinfectant over the spill and allow to sit for 10 minutes. Wipe over general area again with paper towel dampened with disinfectant. pneumoniae that has been dropped onto the floor would be considered a "major biohazard spill". 6. 6. Laboratory Operations & Safety Protocol 47 of 65 V1:2010 . Remove any contaminated clothing and wash any contaminated body surfaces. 5. Hold breath. mop up spill and place contaminated materials into autoclave bag. 4. Dispose the absorbent material by sealing them in a plastic bag and placing it in a trash bin. 1. Do not pour disinfectant directly onto the spill as this may create additional aerosols. Remove and replace any contaminated protective clothing. Discontinue working in the immediate area. face masks and safety glasses" before entering the spill area. 4. coli that has been knocked over on the bench would be considered a "minor biohazard spill" whereas a 500 ml broth containing S. feces.The volume of liquid spilt. In the event of spillage. 8. 5. urine.8 Body Fluids Body fluids include blood. After 10 minutes. 5. 3.

If the injured person is not able to do so.Appendices A. The FORM A.2 in following pages serves as the Accident Report form. as long as there are still faculty in the building to let you out of the building.m. If your supervisor allows it. fill-in. Try to work out a schedule that will go from 6:00 a. Laboratory Operations & Safety Protocol 48 of 65 V1:2010 . If an experiment requires longer than 15 hours. As a rule. Photocopy duplicates (for the Department and for yourself).m. overnight stays are discouraged. At least two people should stay for overnight work. Overtime forms may be processed within the day of the scheduled overtime. It is the supervisor’s (faculty’s) responsibility to ensure that the student receives prompt treatment of the injury by obtaining first aid or assistance to medical treatment. while overnight forms must be handed in to the Department secretary at least 2 days before the event. a co-worker should notify the supervisor as soon as possible. The FORM A. A.m. and submit to the secretary of the Chemistry Department.m. and for yourself). overnight stay shall be considered. or medical treatment in excess of first aid will be investigated by the Chemistry Department to determine liability or the possible need for corrective action. fill-in. The FORM A.2 Form for Reporting Lab Accidents All personal injuries and accidents should be reported in order that corrective action may be taken to minimize the probability of recurrences.1 in following pages serves as the Overtime/Overnight Request form. It is the student’s/researcher’s responsibility to notify his supervisor immediately of any work-related injury or illness. work that will go beyond 12 midnight is considered overnight.3 serves as the Instrument Authorization form. These require you to fill in the form below and seek final approval from the Department Chair. Photocopy duplicates (for the Department and for yourself). A. This will allow the office to inform the University administration and security. Photocopy triplicates (for the Department.1 Form for Overtime or Overnight Lab Work Schmitt Hall is opened to the public by the building manager by 6:00 a. and locked by the security guard at 10:00 p. you may work until 10 p. to 10:00 p. is considered overtime..3 Instrument Authorization Form Students who wish to use an instrument in the department are required to be checked-in first by the faculty-in-charge. It is also the supervisor’s responsibility to file an injury/accident report with the Chemistry Department. and submit to the instrument manager of the Chemistry Department. the Physical Plant.m. File injury/accident reports with the secretary of the Chemistry Department.m. and submit to the secretary of the Chemistry Department. Any injury resulting in lost work time. fill-in. hospitalization. Work that will go beyond 10 p.

After filling-in the above items.1 : Overtime/Overnight Request Full Name (please print) Date of Request Nature of Request (encircle one) Overtime Overnight Time of Event Date of Event Name of Accompanying Person(s) Signature(s) List of Special Equipment.FORM A. and Methods Reason: State why this work cannot be done during regular building hours. get the following signatures: (print name and sign) Your Signature Your Supervisor Building Manager Chair Note: Trplicate! Laboratory Operations & Safety Protocol 49 of 65 V1:2010 . Chemicals.

FORM A.2 : Accident Report
Full Name (please print) Date of Report

Lab Class and Section

Time of Accident

Date of Accident

Name of Injured Person(s)


List of Equipment & Chemicals Involved

Narrate the accident chronologically and in detail. Supply all the pertinent facts.

What first aid or medical attention were applied?

After filling-in the above items, get the following signatures: (print name and sign) Your Signature Witness 1 Witness 2 Chair

Note: Duplicate!

Laboratory Operations & Safety Protocol

50 of 65


FORM A.3 : Instrument Authorization Form
Ateneo de Manila University School of Science and Engineering Department of Chemistry
INSTRUMENT AUTHORIZATION FORM Student:____________________________________ Instrument:_____________________ Name of Mentor:_____________________________ Mentor’s Approval:______________ Purpose of Instrument Use: ___________________________________________________ __________________________________________________________________________ Waiver: I have read and I fully understand the “Policies on the Use of the Department’s Facilities and Equipment.” I _______ agree to follow the guidelines and procedures _______ do not agree to follow the guidelines and procedures because:______________

INSTRUMENT CHECK-IN Date of Check in: ________________________ Faculty in-charge: ________________________ ____Instrument General Working Principles ____Instrument Parts ____ Safety and Precautionary Measures ____ Short Notes of the Procedure ____ Important Reminders and Considerations Remarks:____________________________________________________________________---------------------------------------------------------------------------------------------------------------AUTHORIZATION CERTIFICATE Date: __________________________________ Faculty in-charge: ___________________________ I hereby certify that Mr./Ms. _____________________________ is authorized to use the ____________________________________. Limitations of use:_______________________________________________________________ -------------------------------------------------------------------------------------------------------------------------INSTRUMENT CHECK-OUT Date of Check out:____________________ Faculty in-charge: _________________________ ____Instrument Parts ____Instrument Accessories ____Consumables ____Working environment

Laboratory Operations & Safety Protocol

51 of 65


Figure 1: Lab benches for General Chemistry Lab. Each bench has its own locker, equipment set, hood, sink, power and gas lines, and broom for sweeping up broken glass.


2: Fume hoods for (A) the Analytical Lab, and (B) General Chemistry Lab. The fume hoods are located either on the sides of the laboratory rooms (A), or individually, for each lab bench (B).

Figure 3: Power, gas,
and water lines. These are color coded as follows: RED for power lines, GREEN/BLUE for water lines, and YELLOW for gas lines. The valves are also similarly color coded.


Laboratory Operations & Safety Protocol

52 of 65


Figure 4: Fire extinguishers (A) and showers (B) are placed prominently in all labs. as shown here in the Organic Lab (A) and General Chemistry Lab (B). Laboratory Operations & Safety Protocol 53 of 65 V1:2010 . B Figure 6: Aspirator set-up for the clean-up of mercury spills. A Figure 5: with exits also in all Floor plans directions to fire (red arrows) are placed prominently rooms.

Figure 7: The new (March 2004) fire alarm system in Schmitt Hall: Breaking the cover and pushing the fire alarm button triggers the alarm. Occupants are requested to be familiar with the location of the push button nearest their office/lab. There are several throughout the building. Figure 8: GHS Pictograms and Hazard Classes Laboratory Operations & Safety Protocol 54 of 65 V1:2010 .

Figure 9: Compressed gas tanks tethered properly and capped. Figure 10: Logical computer hardware setup Laboratory Operations & Safety Protocol 55 of 65 V1:2010 . Note the dolly on the right for proper transportation of gas cylinders.

Figure 11: Overview of CMIS Requesting Procedure Laboratory Operations & Safety Protocol 56 of 65 V1:2010 .

Environmental Management Bureau (EMB) Chemical waste management of toxic and hazardous wastes (See Appendix). senior faculty 4-Administrators. List of laws. Regulation on the use. nitric acid. formulation and application of standards for the transport and disposal of effluent. technician-in-charge of CMIS Table 2. technicians. 2-TAs. development of guidelines for re-use of wastewater etc. storage and disposal of toxic chemicals and hazardous and nuclear wastes Air emissions. sewage and septage offsite. EMB Implementing Agency Philippine National Police Coverage Restriction on purchase of nitrates. etc. junior faculty 3-Department secretary. movement. RAs. Law. Rule or Regulation RA 8294: Regulation on explosives and materials used in the manufacture of explosives (1997) RA 6969: Toxic Substances and Hazardous and Nuclear Wastes Control Act (1990) DAO 92-29: Implementing Rules and Regulations of RA 6969 RA 8749: Philippine Clean Air Act (1999) DAO 00-81: Implementing Rules and Regulations of RA 8749 RA 9275: Clean Water Act (2004) DAO 05-10: Implementing Rules and Regulations of RA 9275 EMB Water quality management in all water bodies. rules and regulations which govern various aspects related to chemicals. lab managers. CMIS User-levels CMIS Feature Access to chemical information Access to storage locations Access to stock levels Setting up and modification of data Requesting services Chemical request management Password management Reporting services Administrative functions Access to Hazardous Waste Inventory System (HWIS) 1 x User-level 2 3 x x x x x x x x x x x x 4 x x x x x x x x x x x 1-Guest: for undergrad and grad students. Abatement and control of pollution water bodies.Tables Table 1. ban on incineration Laboratory Operations & Safety Protocol 57 of 65 V1:2010 .

storage and disposal of asbestos-containing materials or asbestos containing wastes. Sulfuric acid 9. storage. 3. distribution. Treatment. Phenylacetic acid 7. sale. collection. Acetone 2. PCB equipment. delivery. Importation. treatment and disposal of PCB wastes. 1-Phenyl-2-propanone 11. possession or use of prohibited and regulated drugs and precursor chemicals RA 6425: Dangerous Drugs Act (1972) Philippine Drug Enforcement Agency (PDEA). Piperidine 8. use and distribution of cyanide and cyanide compounds. and other waste depots. Toluene Laboratory Operations & Safety Protocol 58 of 65 V1:2010 . Piperonal 12. Anthranilic acid 3. PCB-contaminated equipment. Norephedrine 10. sale. N-Acetylanthranilic acid 3. Implementing Rules and Regulations for specific substances Law.RA 9003: Ecological Solid Waste Management Act (2000) DAO 01-34: Implementing Rules and Regulations of RA 9003 EMB Management of solid wastes. Isosafrole 7. Ethyl ether 4. Ephedrine 4. Potassium Permanganate 2* 1. Ergometrine 5. Importation. Methyl ethyl ketone 6. Lysergic acid 8. This also includes the use and possession by electric utilities and suppliers and the generation. non-PCB equipment. administration. formulation of guidelines and targets for solid waste avoidance and volume reduction and setting up of controlled dumpsites. Regulation of the importation. manufacture. List of PDEA-controlled chemicals 1* 1. transport. manufacture of asbestos. distribution and use of PCBs. transportation. DAO 00-02: Chemical Control Order for Asbestos EMB DAO 04-01: Chemical Control Order for Polychlorinated Biphenyls (PCBs) EMB Table 4. storage and disposal of cyanide-bearing or cyanide contaminated wastes. storage. Hydrochloric acid 5. Rule or Regulation DAO 97-39: Chemical Control Order for Cyanide and its Compounds Implementing Agency EMB Coverage Importation. Acetic anhydride 2. transport. PCB articles and PCB packaging in commercial buildings and facilities. This includes proper segregation. Department of Health (DOH) Table 3. manufacture. transfer. treatment and disposal of solid waste. sanitary landfills.4-Methylenedioxyphenyl-2-propanone 9. Treatment. Ergotamine 6.

0 mg/L based on analysis of an extract Includes all wastes with a total Cr concentration > 5.0 Hydrofluoric acid with pH = 2.0 Mixture of sulfuric and hydrochloric acid acid with pH = 2.5 Alkaline cleaners with pH = 12. Wastes with inorganic chemicals Selenium and its compounds Arsenic and its compounds Barium and its compounds Cadmium and its compounds Chromium compounds Lead compounds Sulfuric acid with pH = 2.0 mg/L based on analysis of an extract B201 B202 B203 B204 B205 B206 B207 B208 B299 C301 C302 C303 C304 C305 C399 D401 D402 D403 D404 D405 D406 Laboratory Operations & Safety Protocol 59 of 65 V1:2010 .13.0 mg/L based on analysis of an extract Includes all wastes with a total Cd concentration > 5. Pseudophedrine 14.0 Other inorganic acid with pH = 2.0 Phosphoric acid with pH = 2.0 Acid wastes other than B201 to B208 with pH= 2. Alkali wastes Caustic soda Potash Alkaline cleaners Ammonium hydroxide Lime Slurries Other alkali wastes D.0 Organic acid with pH= 2. Table 5.5 Alkali wastes other than C301 to C306 with pH = 12.0 mg/L based on analysis of an extract Includes all wastes with a total As concentration > 5.0 Caustic soda with pH = 12.0 mg/L based on analysis of an extract Includes all wastes with a total Pb concentration > 5.5 Potash with pH = 12.0 mg/L based on analysis of an extract Includes all wastes with a total Ba concentration > 100.5 Lime slurries with pH = 12.5 Ammonium hydroxide with pH = 12. DENR Classification of Hazardous Wastes Class A: Wastes with cyanide Wastes with cyanide Description Waste Number A101 Waste containing cyanide with a concentration >200 ppm in liquid waste B: Acid wastes Sulfuric acid Hydrochloric acid Nitric acid Phosphoric Acid Hydrofluoric acid Mixture of sulfuric and hydrochloric acid Other inorganic acid Organic acid Other acid wastes C.5 Includes all wastes with a total Se concentration > 1. Safrole *The salts of the substances in the list whenever the existence of such salts is possible (The salts of hydrochloric acid and sulfuric acid are specifically excluded).0 Hydrochloric acid with pH = 2.0 Nitirc acid with pH = 2.

2. it generates toxic gases. copper compounds. It forms potentially explosive mixtures with water. inorganic fluorine compounds excluding calcium fluoride Includes all wastes that are known to contain oxidizing or reducing agents in concentration that cause the waste to exhibit any of the following properties: 1. vapors and fumes in a quantity that poses a danger to human health Includes all wastes that are known to contain oxidizing or reducing agents in concentration that cause the waste to exhibit any of the following properties: 1. When mized with eater. tellurium and its compounds. which when exposed to pH conditions between 2 and 12. thallium and its compounds. It is normally unstable and readily undergoes violent change without detonating. It is normally unstable and readily undergoes violent change without detonating.5 can generate toxic gases.continuation (Table 5) Mercury and mercury compounds Other wastes with inorganic chemicals Includes all wastes with a total Se concentration > 0. vapor or fumes in a quantity sufficient to present a danger to human health. It is a cyanide (CN) or sulfide (S) bearing wastes. vapor or fumes in a quantity sufficient to present a danger to human health. which when exposed to pH conditions between 2 and 12. When mized with eater. Refer to CCO Wastes containing the following chemicals: antimony and its compounds. beryllium and its compounds. 2. 3. It reacts violently with water.5 can generate toxic gases.2 mg/L based on analysis of an extract. Reactive chemical wastes Oxidizing agents E501 Reducing agents E502 Laboratory Operations & Safety Protocol 60 of 65 V1:2010 . it generates toxic gases. zinc compounds. 4. 3. It reacts violently with water. metal carbonyls. 4. vapors and fumes in a quantity that poses a danger to human health D407 D499 E. It is a cyanide (CN) or sulfide (S) bearing wastes. It forms potentially explosive mixtures with water.

soaps. Laboratory Operations & Safety Protocol 61 of 65 E503 E599 F601 F602 F603 F610 F699 G703 G704 V1:2010 .1. ethyl ether. Waste organic solvent Halogenated organic solvents Includes the ff. D502. caustic washings and sludge or wastewater and sludge from cleaning of tubs and equipment used in the formulation of ink from pigments. tricholorethylene. spent halogenated solvents: Tetrachloroethylene. Highly reactive chemicals Includes all other wastes that exhibit any of the properties described for D501. methyl isobutyl ketone. ethyl acetate. nitrobenzene. pyridine. cresylic acid. and D503. Carbon disulfide. G. it also includes all still bottoms from recovery of these solvents and solvent mixtures. driers. carbon tetrachloride. 1. n-butyl alcohol. cresole. Trichloroethane. chlorinated flouro-carbons if they contain a total of 10% or more (by volume) of one or more of the above before use. it also includes all still bottoms from recovery of these solvents and solvent mixtures. 1. 2-ethoxy ethanol and 2 nitropropane and other non-halogenated organic solvents if they contain a total of 10% or more (by volume) of one or more of these solvents before use. Non-halogenated organic solvents Includes the ff. methanol.continuation (Table 5) Explosive and unstable chemicals Includes all wastes that are 1) capable of detonation or explosive reaction when subject to a strong initiating source or when heated under confinement. chlorobenzene. Other mixed Includes all aqueous-based wastes that also meet one or more of the subcategories. non-halogenated solvents: Xylene. benzene.hexanol. cyclo.isobutanol. F:Inks/Dyes/Pigments/Paint/Latex/Adhesives/Organic sludge Aqueous based Includes all aqueous based wastes that also meet one or more of the subcategories Solvent based Includes all solvent based wastes that also meet one or more of the subcategories Inorganic pigments Includes all wastewater treatment sludge from the production of inorganic pigments Ink formulation Includes all solvent washings and sludge. acetone. or 2) capable of detonation or explosive decomposition at a temperature of 20 o Celsius and Pressure of 1 atm.2. and stabilizers containing Chromium and Lead. ethyl benzene. methylene chloride.1.2 Trichloroethane. toluene.

All wastes from commercial slaughter houses that slaughter an average of 500 or more animals per year . Wastes whose hazardous substances are physically immobilized by consolidation to reduce the surface area of the wastes in order to meet the waste acceptance criteria Wastes whose hazardous substances are chemically immobilized through chemical bonds to an immobile matrix or chemical conversion to meet the waste acceptance criteria Wastes whose hazardous substances are physically immobilized by enveloping the waste in a non-porous. Includes all establishments that generate an average of 50 kg per day Includes all wastes from establishments that generate. all waste from facilities that process an average of 2500 fowls or more. and the chemicals listed in the Priority Chemical List.continuation (Table 5) H. D. Oil Waste oils Includes all wastes from animal feed lots containing an average of 100 or more animals.000 fowls or more. all waste from poultry farms with an average of 5. J201 K: Immobilized Wastes Solidified wastes and polymerized wastes K301 Chemically fixed wastes K302 Encapsulated wastes K303 Laboratory Operations & Safety Protocol 62 of 65 V1:2010 . E. Containers that used to contain Polychlorinated biphenyl (PCB) are categorized as L406 and excluded from this sub-category. impermeable material in order to store hazardous wastes until such time that a proper disposal facility is available. transport or treat more than 200 L of waste oil per day except vegetable oil and waste tallow H801 H802 I101 J: Containers Containers previously containing toxic chemical substances Waste containers that used to hold the toxic chemical substances listed in Classes A. Putrescible/Organic Wastes Animal/abattoir waste Grease trap wastes from industrial or commercial premises I. and L. sub-categories M504 and M505.

fetuses.2-diphenylhydrazine benzene Waste chlorofluoro carbons (CFCs) and halons. endrin. Waste blue and brown asbestos fibers. Includes all wastewater sludge from production of pesticides other than those listed in M505. Refer to CCO. L401 Ozone depleting substances L402 PCB wastes L406 M: Miscellaneous Wastes Pathogenic or infectious wastes Includes pathological wastes (tissues. toxaphene. bloods and body fluids). Waste pesticides other than M505. Expired pharmaceuticals and drugs stocked at producers and retailers’ facilities. and DDT.1.organs. infectious wastes and sharps Wastes containing friable asbestos. mirex. Waste pesticides listed in the Stockholm Convention (POPs Convention) such as aldrin. chlordane. dieldrin. hexachlorobenzene.continuation (Table 5) L: Organic chemicals Wastes with specific nonhalogenated toxic organic chemicals Non-liquid waste containing the following: . heptachlor. Refer to CCO. Recovered coolant containing chlorofluoro carbons (CFCs) or halons Wastes contaminated with PCB and waste products containing PCB.Tri-butylin . M501 Friable asbestos wastes M502 Pharmaceuticals and drugs M503 Pesticides M504 POPs (Persistent Organic Pollutants) pesticides M505 Laboratory Operations & Safety Protocol 63 of 65 V1:2010 .

Table 6. Summary of Recommended Biosafety Levels for Infectious Agents1 Laboratory Operations & Safety Protocol 64 of 65 V1:2010 .

Job description for Technical Assistant Date Accessed: 6 June 2002. School of Science and Engineering).gov/SLTC/hazardoustoxicsubstances/) Date Accessed: 17 June 2010. in 18. Dr. 13. Memo to the Loyola Schools Community.flinnsci. 40 Steps to a Safer Laboratory (http://www. Laboratory Safety Manual. Health and Safety Office.html) Date Accessed: 6 June 2002. 20 July 1987. Procedural Manual Title III of DAO 92-29. 11. 5 August 2002. Princeton University.osha. 7. 2004 21. Anna Miren Gonzalez-Intal (VP Loyola Schools). February 1992. 1997 Policies and Standards for B. 2.princeton. 24. 19. Less is Better. University of Wyoming. 3. Microbiology Safety and Staff Induction Manual. Judith Wormal. Access Date: 6 June 2002.htm).S. 15. Dr. 9. Hazardous and Toxic Substances.htm) Date Accessed: 21 November 2002. Laboratory Operations & Safety Protocol 65 of 65 V1:2010 . Francis Ted Limpoco.1200 App A (http://www.osha. Health Hazard Definitions (Mandatory) –1910. 9 August 2002. 10 September 1996. 7 March 2006. Environmental Health and Safety (http://web. Safety Practices in the Department of Chemistry. General Chemistry Lab (Ch 8) Course Outline & Lab Policies. Research and Teaching Assistants. Ana M. Dayrit (Dean.labsafety. Students. University Chemical Management Plan. Dr.labsafety. 23.acs. 2008 5. School of chemical Sciences. Fabian M. Proposal. Ma. Memo to the Chemistry Faculty. on Fire and Evacuation Drill on Friday. Javellana (Chair). 22. Undergraduate Teaching Lab Safety Contract Sample (http:// www.References 1. 20. What To Do When Handling Body Date Accessed: 6 June 2002. Chemical Management and Inventory System Manual. OSHA Fact Sheet of Hazardous Chemicals in Labs (http://www. Chemical Hygiene Date Accessed: 6 June 2002.acs. February 2000. June 2002. Contact Lenses and Chemicals ( Access Date: 6 June 2002. University of North Carolina at Chapel Hill. University of North Carolina at Chapel Hill. Ateneo de Manila University. 12. 6 August 1979. Nestor S. Occupational Safety and Health Administration (OSHA) (http://www. Frequently Asked Safety Questions (http://www. 17. on Operating Practices. 6. 4. Cuyegkeng (Chair) Date Accessed: 6 June 2002. Guide to Minimizing Waste in Laboratories ( biosafetypage/section4. 8. Dr. University of Illinois. 10. January 2010. Access Date: 14 June 2010. 2 May 2001. National Research Council Recommendations Concerning Chemical Hygiene Laboratories (http://www. Staff. Memo to the Chemistry Faculty. University of Tasmania .org) Access Date: 6 June 2002. on Safety Policies. Valera (Chair).Launceston Campus. Assunta C. 16. Chemistry (http://www. “Hazardous Waste Management” DENR AO 36.

Sign up to vote on this title
UsefulNot useful