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Chemistry Department Loyola School of Science & Engineering Ateneo de Manila University Version 2010
Preface to the 2010 Edition
In 2004, the government started to implement various laws, rules and regulations covering a broad range of activities related to the purchase, use, storage, waste management, and disposal of chemicals. The increased regulation has led the University to implement a common policy and procedure to address these various concerns. The Chemical Management and Inventory System (CMIS) was developed to address the need for accurate inventory, efficient purchasing, management, and compliance with various laws as well as the management of hazardous chemical wastes. The changes in the legal and regulatory climate have also contributed to the changing culture of safety which now puts emphasis on experiment planning, risk assessment, hazard consideration and chemical waste minimization. In this edition, information on different sections (i.e. Emergency Protocols, Use of Instruments and Waste Disposal) was updated. A section on the CMIS and Handling of Biological Samples was added. I wish to thank Ms. Mailyn Terrado for the section on Dealing with Biological Samples and Wastes. I also wish to thank Dr. Regina C. So and Dr. Fabian M. Dayrit for taking the time to proofread the manual. Jaclyn Elizabeth Santos 18 June 2010
Preface to the 2002 Edition
his manual is intended to be a safety reference document for lab workers at the Chemistry Department of the Ateneo de Manila University. It is mainly adapted from the Laboratory Safety Manual at the University of North Carolina at Chapel Hill, and the memo on Operating Practices issued by Dr. Ma. Assunta Cuyegkeng when she was Chair of the Department in 1996—practices that have served the Department well since the time of Fr. Schmitt. This manual provides basic information about hazards encountered in the lab and safety precautions to prevent accidents. It also contains the description of faculty, student, and staff roles in ensuring a safe working environment in the lab. It is not possible to cover all safety and emergency scenarios, but this manual should, nonetheless, serve as the starting point for the faculty in developing their own safety plans specific to the materials and procedures in their laboratories. It is often a sticky effort to balance convenience and control with regards to safety issues in the lab, but when all is said and done, the basic rules are alertness and vigilance, common sense, conservation of resources, and concern for other workers in the lab.
Francis Ted Limpoco
18 November 2002
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1.1 1.2 1.3 1.4 1.5 Safety Awareness The Role of Faculty The Role of a Safety Officer The Role of Lab Technicians The Role of Students/Researchers
6 6 7 8 9
2. Emergency Protocols
2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 Dealing with Emergency Emergency Telephone Numbers Fire Emergency Chemical Spill Escape of Noxious Gases First Aid Medical Attention After First Aid Reporting Accidents and Injuries
10 11 12 13 14 14 15 16
3. The Use of Building & Facilities
3.1 3.2 3.3 3.4 3.5 3.6 Schmitt Hall Room Addresses Use of the Building Access Keys Special Rooms Use of Instruments
17 18 19 19 20 20
4. General Safety Principles
4.1 4.2 4.3 4.4 4.5 4.6 4.7 4.8 4.9 Overnight Operations Working Alone Eating, Drinking, and Smoking Housekeeping Warning Signs and Labels Shielding for Safety Compressed Gases Systems Under Pressure Cold Traps and Cryogenic Hazards
23 23 23 23 23 24 24 24 25 25
5. Chemical Management and Inventory System
5.1 5.2 Chemical Management and Inventory System Controlled Chemicals
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7 Clean-up Procedure 8.3Transport 8.4 Labeling 7.6 Waste Disposal 8.5 Handling Carcinogens 6.4 Handling Toxic Materials 6.4 Handling 8.3 Handling Specific Wastes 7.7 Handling Explosive/Reactive Materials 30 30 32 33 35 36 37 38 7.6.7 Disposal of Chemical Wastes 40 40 40 40 41 41 41 42 8.2 Waste Reduction 7.1 Hazardous Chemicals 6. Handling of Chemicals 6.3 Protective Clothing and Equipment 6.6 Handling Flammable Materials 6. Biological Samples: Handling and Waste Disposal 44 8.2 Routes of Exposure 6. Waste Disposal 7. Gas. and Water Lines Fire Extinguishers & Showers Floor Plans with Fire Exits Aspirator Set-up for the Clean-up of Mercury Spills Fire Alarm Compressed Gases CMIS’ Logical Computer-Hardware Set-up Overview of Requesting Procedure Overview of CMIS Requesting Procedures 52 52 52 52 53 53 53 54 54 55 55 56 Laboratory Operations & Safety Protocol 4 of 65 V1:2010 .5 Waste Containers 7.3 Form for Overtime/Overnight Lab Work Form for Reporting Lab Accidents Instrument Authorization Form 48 48 48 48 Figures Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 Figure 7 Figure 8 Figure 9 Figure 10 Figure 11 Lab Benches Fume Hoods Power.5 Laboratory Equipment 8.8 Body Fluids 44 44 44 44 45 46 46 47 Appendices A.2 Storage 8.6 Disposal to Sewerage System 7.1 A.1 General Information 8.1 Waste Characteristics 7.2 A.
rules and regulations which govern various aspects related to chemicals 57 Table 3 Table 4 Table 5 Table 6 Implementing Rules and Regulation for specific substances List of PDEA-controlled chemicals DENR Classification of Hazardous Wastes 58 58 59 64 Summary of Recommended Biosafety Levels for Infectious Agents References 65 Laboratory Operations & Safety Protocol 5 of 65 V1:2010 .Tables Table 1 CMIS User-levels 57 57 Table 2 List of laws.
b. Every instructor should: 1. and power lines. Inspect the equipment in the students’ and common lockers. c. This attitude can lead to a false sense of security. be alert to unsafe conditions and actions and call attention to them so that corrections can be made as soon as possible. This includes showing the locations of the fire extinguisher. Please do not argue. Remind students to settle the lab breakage deposit immediately. Laboratory Operations & Safety Protocol 6 of 65 V1:2010 .1 Safety Awareness he most important rule is that everyone involved in lab operations—from the highest administrative level to the individual workers—must be safety minded. and the locations of the valves for the gas. Not eating or drinking during the lab. 3. but also the other people involved— always consider their safety and convenience as well. eyewash. closed-toe shoes and apron). and evacuation plan. c. water. and check-out the same at the end of the term. (See Figures 1-5.Chapter 1 Introduction T 1. Brief students on the lab operations and safety protocols. You must consider not just yourself. 2. b. safety glasses. the faculty should be in the laboratory for the entire laboratory period. Safety awareness can become part of everyone’s habits only if the issue of safety is discussed repeatedly and only if senior and responsible staff demonstrate a sincere and continuing interest in safety. Ask if students have special medical conditions that may interfere with lab work/safety. Most of the rules/responsibilities stated here are nothing more than common sense and concern for others.) 4.g. Set a good example by being enthusiastic about safety. such as: a. which frequently results in carelessness. NO SET OF RULES can cover all situations. Check-in students at the start of the term. Common sense is still the guiding norm. As the saying goes: “An ounce of prevention is worth more than a pound of cure”. Maintain discipline and enforce the Department’s operational and safety rules.2 The Role of Faculty The faculty is responsible for the administration of the safety program of the Chemistry Department. Wearing the prescribed safety gear: safety glasses and aprons. Every lab worker has a basic responsibility to himself and his colleagues to plan and execute lab operations in a safe manner. Orient students in the proper use of the lab facilities and instruments. Not entering the stockroom. Thus. 1. d. “We were not told.” when it is obvious what you should do in a given situation. shower. Over familiarity with a particular laboratory operation may result in overlooking or underrating its hazards. a. and by observing all safety rules and wearing personal protective equipment (e. As lab instructor.
5. but also ensures that the student is not exposed to safety risks in doing his research.5. eye wash stations. fire extinguishers. stating the cause. Regularly orient science majors (both undergraduates and graduate students. and recommendations for the prevention of reoccurrence.g. Train students in proper lab techniques. etc. 7. waste minimization and where appropriate. storage and disposal of the Chemistry department. Conduct an inventory of chemical wastes generated by the department. redesign or replacement. and by observing all safety rules (e. spill kits) 4. Accidents resulting in medical treatment or observation should be recorded. Conduct a briefing on safety issues at the start of the class. The mentor/supervisor should: 1. hoods. Orient the students on the chemical waste disposal procedures of the department. fire alarms. inspect often and intelligently. Coordinate with the University Physical Plant and Office of Adminitrative Service regarding the disposal of chemical wastes with a DENR-accredited waste transporter and treater.g. 10. safety showers. where recommended). 2. 8. 2. 6. Orient researchers with the general operations and safety procedures at the Chemistry Department. 3. Report all accidents. A formal written report to the Chair should be made of all accidents. Set a good example by being enthusiastic about safety. eyewear. 9. compliance recommend appropriate improvements. The mentor is responsible for checking-in researchers on the instrument or ensuring that they are checked-in by a competent/expert user of the instrument. In case of accidents/emergencies. effect. Determine whether researchers need to work continuously after office hours or overnight. Discuss with researchers any safety risks involved in the experimental procedures that will be carried out and the materials that will be used in the research. especially those that may pose safety hazards. and Laboratory Operations & Safety Protocol 7 of 65 V1:2010 . aprons. check that the system is working satisfactorily. Undertake an annual review of the laboratory experiments which are included in curricular offerings to consider aspects of safety. The safety officer should: 1. be alert and vigilant for unsafe conditions.3 The Role of a Safety Officer The safety officer is responsible for activities related to safety. Carefully review all laboratory experiments for possible safety problems before experiments are assigned. Anticipate and prevent accidents at all times. 1. Review and update safety protocol and procedures in the department. 6. Notify the parents/guardian of the injured person about the incident. 7. and ensure there are logistical provisions for their safety in such cases.e. Together with the laboratory supervisers and staff. 8. 3. first aid kits. no eating and drinking in the lab/stockroom) and wearing protective equipment (e. implement proper first aid measures and if necessary coordinate proper and speedy conveyance of the injured person to a medical facility. the faculty not only guides the student in the direction of his research project. Manage the CMIS. Orient researchers with the instrument techniques and protocols. 4. regularly check safety facilities and equipment (i. especially those doing their thesis) research assistants and faculty members regarding chemical safety and chemical waste management and minimization. and take effective corrective action promptly. As mentor/research supervisor. chemical management.
effect. especially for laboratories. Laboratory Operations & Safety Protocol 8 of 65 V1:2010 . assist the faculty/lab supervisor in implementing proper first aid measures and if necessary assist in the proper and speedy conveyance of the injured person to a medical facility. glassware repairs. and others. first aid kits. f. eye wash. investigate and repair simple electrical circuits and minor electrical breakdown. In case of accidents/emergencies. and recommendations for the prevention of reoccurrence. According to established norms. fine students for failure to observe laboratory rules or inform the lab instructor of any violations to enforce penalty. c. A formal written report to the Chair should be made of all accidents. d. stating the cause. supplies. and deliveries. wood.9. no eating and drinking in the lab/stockroom) and wearing protective equipment (e. such as: fire extinguishers. mechanical repairs. 1. Remind students to settle their lab breakage deposits immediately. and repairs on metal. and sand for cleaning up spills. Manage the stock of chemicals and materials: keep records of materials. fume hood. 3. Ensure that teaching laboratories are provided with properly-labeled chemical waste bottles.. Under the supervision of faculty member. e. Handle the renewal of licenses required by government authorities. first aid kits. where recommended). Accidents resulting in medical treatment or observation should be recorded. c. 7. a. The lab technician should: 1. materials. 2. 6. Provide technical services at Schmitt Hall. and that these are placed in easily accessible locations in the laboratory.g. Report all accidents. Inform the immediate supervisor well in advance of the need to order supplies before they run out. Ensure that the CMIS is updated. 4. Regularly check current loads of aircons. aprons. do various operations such as purification of solvents by distillation. Prepare with accuracy chemicals and solutions for laboratory work. a. b. eyewear. 10. Dispense chemicals. Exercise supervision over students with regard to the stockroom transactions and general order in the laboratories. etc. inspect all safety implements according to the recommended frequency. Together with the safety officer. Prepare a periodic report (annually or as required) regarding the status of the chemical inventory and chemical waste of the department. Together with a trained professional. 5. Assist the faculty in inspecting the student laboratory lockers/equipment at the beginning and at the end of the semester. Set a good example by being enthusiastic about safety. Make plumbing repairs. Prepare chemical unknowns which must be carefully coded and recorded. and plastic. emergency showers.g. Provide thesis students with chemical waste bottles. b.4 The Role of Lab Technicians The lab technician and/or stockroom manager should also be present during the entire laboratory period to assist the faculty in managing the experiment and administering the safety procedures of the Department. special containers for chemical and broken glass wastes. 8. and equipment. Ensure that safety materials and paraphernalia are well in stock and replenished when used up. This includes fire extinguishers. and by observing all safety rules (e.
Follow protocols for handling chemicals and for dealing with spills. Never work alone. Research the hazards and physico-chemical properties of the chemicals used in the experiment. arms. No pranks. having read the experimental procedure in advance. or slippers. Never use your handkerchiefs or face/hand towels to wipe things in the lab. Contact lenses must not be worn. preparations. and treat all chemicals with respect. d. Although the Department aims to provide a safe working environment for its students. Laboratory Operations & Safety Protocol 9 of 65 V1:2010 . Do not perform unauthorized work. c. Wear clothing appropriate for work: Do not wear shorts. Any deliberate behavior that may compromise you and your co-workers’ safety may be grounds for your dismissal from that lab class/course. If in doubt. The first thing you do in entering the lab is to put on your apron the last thing on leaving is to remove it. the student should be responsible for their personal safety when they are working in the lab. Come in the lab prepared. Wear clothing that would protect your body against contact with chemicals. d. d. 6. whether major or minor. 3. c. Safety glasses must be worn inside the laboratory. how to dispense the correct amount of reagents properly. and experiments. or cellphones. reactivity. Always wash hands. c. sandals. fire escape. Wear proper personal protective equipment: a. e. from the work area. b. 4. Read and prepare for remedies in case of exposure. safety shower and eyewash. or apply cosmetics inside the lab or storage areas. Read and understand general safety procedures. b. Consider lab work serious work. 2. use medication. such as bags. Goggles resting on your forehead are not in a position to protect your eyes. You are university students.1. Follow hazard precautions. This should be done even if gloves are used. not preschoolers. and face before leaving the work area. Report all accidents. Aprons or some suitable protection must be worn at all times in the laboratory. Be informed about experimental procedures and appropriate safety concerns: a. first aid kit. or the lab technician in charge. research supervisor. especially with hazardous chemicals. consult with your lab instructor/supervisor.) Learn how to use instruments properly. and those specific to your experiment. books. 7. Avoid unnecessary exposure to chemicals: a. Confine hair and loose clothing. Every student should: 1. and how to dispose of chemical waste properly. smoke.5 The Role of Students/Researchers The student/researcher is responsible for complying with the safety rules and operational procedures of the Chemistry Department. e. drink. b. When you are organized. corrosiveness. you are less likely to commit accidents. (See Figures 1-5. e. or other acts of mischief. and toxicity. Keep a neat and clean work area.g. 5. Know the location of the fire extinguisher. Never eat. Keep personal belongings. to the lab instructor. flammability. horseplay.
Alert others in the area of the accident. follow evacuation procedures under Section 2.1 Dealing with Emergency What to do: 1. Gonzaga (local 5624) 5102) The University security personnel (local 4111. personnel (local 5624 or 5631) The Chemistry Department office (local 5620) 4111. 4113) The following fire departments: o o o o 8363) Marilag Fire Sub-station (913 0125/510 9299) Pinagkaisahan Fire Sub-station (474 2695) New Era Fire Sub-station (931 9894/931 4956) Central Fire station (928 • University personnel (faculty. especially the one in-charge of the lab The lab technician. Try to alleviate the immediate need in the situation without compromising your own safety: • • • Remove an incapacitated co-worker from the accident area Turn off fuel and power lines. evacuate the Note: For fires. 4112. 4112. 4113 ) After office hours: • The University security personnel (local If the incident is out of control (e. Laboratory Operations & Safety Protocol 10 of 65 V1:2010 . or any Chemistry Dept. use fire extinguisher Immediately call for help. 3. 2.Chapter 2 Emergency Protocols 2.3 (Fire Emergency). lab technician.) as above. area and immediately call for help. immediately alert any of the following: During office hours: • • • Any faculty of the Chemistry Department. rapidly spreading fire). If the situation is beyond your control and presents a danger to you. Who do you call for assistance? If the incident is small and contained. disaster-assistance team captain of the building. etc. immediately alert any of the following: • • • • The disaster-assistance team captain of the building (local The Fire Marshall at OAS.g.
2. Fire Departments • • • Quezon City Marikina Mandaluyong 928 8363 933 3076 5322189 Ateneo de Manila University • • Trunk line Telephone Operators 426 6001 local 4199 Chemistry Department Directory • • • • Department Office (Chair/Secretary) Schmitt Hall Stockroom SEC Stockroom Faculty Offices 5620 local 5624 local 5631 local local 5621-23. University Gym) Gate 2 Gate 3 local 4111 local 4112 local 4113 local Office of Administrative Services • Fire Marshall (Gonzaga Hall) 5103 Physical Plant Office • • • • • Administrator Secretary Switchboard Operation Center (South Wing. and must be updated annually. 5625-33 University Security Office • • • Main Office (North Wing. Luke’s Medical Center UP Health Service 372 3825 924 3601 925 2401 723 0301 981 8500 local 112 Laboratory Operations & Safety Protocol 11 of 65 V1:2010 . University Gym) Direct Line local 4100 local 4101 local 4102 local 4104 426 5940 Infirmaries within Campus • • • • Health Service HealthDev (at the Social Development Complex) Jesuit Residence (ask for Infirmary) Loyola House of Studies (ask for Infirmary) local 5110 local 4630-33 local 3399 local 3650 Nearby Hospitals • • • • • Capitol Medical Center National Kidney Institute Philippine Heart Center St.2 Emergency Telephone Numbers Note: These numbers are subject to change.
Secure potential hazards in your area such as open flames. (See Figure 5.) To use the fire extinguisher. 3. Do not re-enter the building unless you are given permission to do so. WARNING: Do not attempt to fight a fire that is spreading rapidly or if you are not sure how to operate the fire extinguisher. Alert others in the area of the fire. b. Leave the building using the nearest stair tower to reach ground level. S – Sweep from side to side. 4. for example. The fire may block your exit. e. d. and evacuate the building. Meet the fire department outside of the building and provide information about any special hazards and other considerations. (See Figure 4.g. and crawl if necessary. ovens. etc. containing CO2. compressed gasses. small appliances. Identify yourself Identify the kind of fire Identify the building and room number Give your telephone number Don’t hang up until the dispatcher has the needed information. remember: Fire Extinguishers. e. 5.) 4.2. Immediately inform any of the following: • • • 5624) The Fire Marshall at OAS.3 Fire Emergency What to do: 1. 2. Provide the following information 3. 5. • • • • P – Pull the pin. 2. If the fire is small and confined to its origin. If you encounter smoke. S – Squeeze the lever. There are emergency exit routes posted on the wall of each room. Gonzaga hall (local 5103 or 5118) Quezon City Fire Department (928 8363 or 928 6535) The disaster-assistance team captain of the building (local a. Evacuation Procedures. c. 1. stay close to the floor. assume that the emergency is real until you are informed otherwise. “Gas” extinguishers. Close windows and doors in your area. offer first defense against flammable liquids or electrical fires without leaving a powder residue which could harm electronic equipment. Most chemical laboratory fire hazards require dry chemical multipurpose extinguishers (ABC) which must be installed in hallways. When alerted of a fire. use the portable fire extinguisher. Close the doors to confine the fire. Laboratory Operations & Safety Protocol 12 of 65 V1:2010 . A – Aim the nozzle.
paper towels are not suitable for cleaning up flammable spills. not highly corrosive. Notify the faculty or lab technician in charge of the class (local 4. When the toxicity of the spilled material is unknown. Spill Clean-Up 1. Add sand to contain the solid spill. A quantity of solid not much greater than the volume of the liquid spilled should be sufficient. If the material is not reactive to water. Use sand/soil to contain liquid spills. warn everyone immediately to extinguish flames. treat the spill like a potential health hazard by avoiding exposure and seeking assistance from a trained personnel. Use. 7. Do not leave paper towels or other materials used to clean up a spill in open trashcans in the work area. and respiratory protection. Exposed skin should be thoroughly washed with soap and water. gloves. 3. 5. 5110). even of concentrated reagents. Seek medical attention (Health Service. Wear appropriate personal protective equipment: goggles. Any contaminated clothing should be removed and containerized. Acids and Bases: Avoid contact with skin. Forcibly hold the eyelids open as necessary. adding some water to provide solvent for the neutralizing reaction. and if applicable. respectively. use an absorbent material that will contain the liquids. 5. Use paper towels and dustpan to collect the spill residue. as appropriate. 7. especially for volatile and toxic spills. Ventilate the spill area. Place in a labeled plastic bucket or container. safety shower or eye wash fountain. For small liquid spills (<100 mL). Dispose them properly. 8.4 Chemical Spill Dropping containers or otherwise spilling chemicals in the open lab can result in exposure to hazardous agents. 2. Do not brush up solid spills since this may produce airborne dusts. 9. Mix neutralizing chemical into the spill. or an absorbent can be used to contain the spill. Immediate Action 1. 3. Check for and remove contact lenses. and there is no fire hazard. when necessary by opening windows or use a fan. has a low order of toxicity. closing the door and warning others not to enter the contaminated area. However. If a volatile.2. Spills of Specific Types of Chemicals 1. a minimum of 20-minute flushing with copious amounts of water is recommended. (See Spills of Specific Types of Chemicals below). flammable or toxic material is spilled. 2. and turn off spark producing equipment. If the material is not particularly volatile. 6. proceed with clean-up operations. Report the incident to faculty or lab technician. Laboratory Operations & Safety Protocol 13 of 65 V1:2010 . Continue flushing with water for 15 minutes or more. For chemical splashes on the eyes. Rotate the eyeballs so that all surfaces are rinsed. sand. Everyone should leave the affected area. you can add water to the spillsand mixture to act as dust-suppressant. These should be laundered separately from other clothing before reuse. local 5624 or 5631). Avoid skin contact and minimize inhalation. paper towels. Neutralize acids and bases with solid sodium bicarbonate and citric acid. 4. It is important to minimize the extent and possible effects of exposure to these hazardous chemicals. Shut down all equipment and vacate the area until it is decontaminated. Use pH paper to determine whether the acid or base have been neutralized. 6.
burn ointments. HF) with silica-containing materials such as sand or vermiculite. First Aid for Specific Types of Accidents 1. and close off the room from the rest of the building.After neutralizing. aniline vapor. The situations described herein are the ones of particular concern in a chemistry laboratory. Final rinse is clear water. Mercury spilled into floor cracks can be made non-volatile by amalgamation with zinc dust. but be warned against absorption through the skin of such substances such as HCN gas. refer to a more extensive first aid manual. Chemicals in the Eyes: A minimum of 20-minute flushing with copious amounts of water is recommended. Forcibly hold the eyelids open as necessary. etc.6 First Aid All members of the Chemistry Department must undergo a yearly first aid and cardiopulmonary resuscitation (CPR) course and should be ready to apply first aid measures to an injured person in a lab. thus increasing the damage to the skin and promoting poisoning through the skin. Take (do not send) the victim to the Office of Health Services (local Sciences Building). 2. Solvents may cause the oil to dissolve in the skin. accordingly. Do not use sulfur to cover mercury since this will just complicate disposal. 5110. Place the source of gas in a hood (and make sure to turn the motor on). 2. antiseptic wash. Chemicals on the Body: Flush areas copiously with water under safety shower or under the sink. The stockroom must also be ready to provide first aid supplies: bandage. Rotate the eyeballs so that all surfaces are rinsed. mopping should follow. Water-insoluble oils must be washed off with soap and water. In any event.. evacuate personnel. Quickly remove chemically soaked clothing. and the burning and poisoning through skin by HF gas. Keep victim warm. Social 2. 3. Heavy vapors (MW >>29) will lie along the floor. 2. Leave the room in either upright or crawling position. WARNING: Do not clean-up hydrogen fluoride (hydrofluoric acid. Laboratory Operations & Safety Protocol 14 of 65 V1:2010 . Modesty should not deter removal of clothing in a true emergency. etc. Watch victim carefully for shock. Mercury: Because of the high toxicity of mercury vapor. spilled mercury should be cleaned-up immediately and thoroughly using an aspirator or vacuum device. (See Figure 6. Contaminated materials used to clean up the spill should also be placed in properly-labeled containers. Rinse mop and bucket.) Domestic vacuum cleaners must not be used.5 Escape of Noxious Gases Immediate Action 1. open windows. cutting or tearing them if necessary to get them off rapidly. WARNING: Do not use solvents to wash off offending oily chemicals. poison remedies. etc. For more general concerns. Check for and remove contact lenses. WARNING: A mask may protect the wearer against inhalation of noxious gases. Very much lighter vapors will rise. or by adding a mixture of finely powdered sodium thiosulfate (85 g) and powdered EDTA (15g). 2.
Symptoms of shock include: cold sweat. Watch victim carefully for shock. There is always the possibility that they may collapse en route. have shock victim lower head between knees. Transporting the Injured Person: No one should be sent to seek medical attention without accompaniment. It may be the most dangerous result of an accident. extreme dullness or sluggishness. vomiting. Cover burned areas in 3% boric acid. shivering and chills. 5110. Remedies include: egg albumin. Take (do not send) the victim to the Office of Health Services (local Sciences Building).7 Medical Attention After First Aid After being administered first aid. Social Fire Burns: Rush victim under safety shower or smother fire on victim with a blanket (e. pounding heart. jacket. Social 2. All these are signs of trouble and require immediate medical attention. The cold shower will suffice. and tannic acid.). 3. nausea. Keep victim warm. 5110. Shock can be fatal! Be on the lookout for it. and first aid precautions to follow in case of chemical poisoning. Social Sciences Building). a victim should not drive a car for several hours after a severe injury. the injured person must be taken (not sent) directly to the Office of Health Services (local 5110. Laboratory Operations & Safety Protocol 15 of 65 V1:2010 . 5110. Watch victim carefully for shock. and shallow breathing. On most fire burns. nausea. Only very minor burns should then receive burn ointment. Keep moist with neutralizing solution soaked on gauzes. charcoal. Shock: Accident victims experience shock. Take (do not send) the victim to the Office of Health Services (local Sciences Building). cold sweat. weakness of the arms and legs. Keep victim warm.g. WARNING: Application of cold to large areas of the body produces shock. Take (do not send) the victim to the Office of Health Services (local Sciences Building). extreme paleness of the face. Also. The antidotes required. do not apply burn ointment. trembling. Keep victim warm. 1. Signs of Danger: Watch the victim for blood leaving the head (face turning white) or excess blood in the head (face turning red). Watch victim carefully for shock. To restore blood to the head. jittery talk. 2. have him lie with head lower than the body. Burned areas should be cooled immediately. and difficulty in breathing. very rapid pulse. heavy sweating. Keep victim warm. magnesium sulfate. Keep moist. shallow rapid breathing. etc. Watch victim carefully for shock. Social 4. it seals the chemicals under the greasy coating. Also refer to first aid wall charts. hence the advantage of using the safety shower. mustard powder. The supervisor (faculty/lab technician) or a co-worker must accompany the injured person in seeking medical treatment. even though at the time you dismiss them they assure you they feel fine and are quite ready to go alone. or better.Follow water-washing with the correct neutralizing solution. etc. as 3% boric acid (remedy for base) or 5% bicarbonate (remedy for acid). lab coat. Soak affected part in ice water for 20 minutes to reduce deep burning. Chemical Poisoning: Get poison remedies/antidotes from the stockroom manager or lab technician. are usually printed in red letters on the bottle labels. Treatment with ice water is not recommended for large-area burns. WARNING: Do not apply greasy burn ointment to chemical burns.
If he vomits. for demonstrating whether faculty/staff were negligent in preventing the accident or careless in subsequent treatment of the victim. It is the supervisor’s (faculty’s) responsibility to ensure that the student receives prompt treatment of the injury by obtaining first aid or assistance to medical treatment. apoplexy. Give oral stimulant (hot tea or coffee) if conscious. or medical treatment in excess of first aid will be investigated by the Chemistry Department to determine liability or the possible need for corrective action. for establishing legal responsibility for an accident. 3. for establishing a claim for damages of insurance benefits. Do not give stimulant until bleeding is controlled. Any injury resulting in lost work time. a coworker should notify the supervisor as soon as possible. 2. showers. Report the Use of Safety Equipment: Immediately report the use of eye wash. and not at all in case of fractured skull.2) are of the utmost importance for correcting procedures. 3. It is the student’s/researcher’s responsibility to notify his supervisor immediately of any work-related injury or illness. be sure his air passages do not become blocked. elevate the head rather than lower it! This may occur in the case of sunstroke.8 Reporting Accidents and Injuries All personal injuries and accidents should be reported in order that corrective action may be taken to minimize the probability of recurrences. or sunstroke. 2. hospitalization. fractured skull. neutralizing chemicals. or hemorrhage of the head. fire extinguishers. etc. abdominal injuries. It is also the supervisor’s responsibility to file an injury/accident report with the Chemistry Department. WARNING: It is still best to take the victim to see a physician first before giving any oral remedy.WARNING: If the face is flushed or red. If the injured person is not able to do so. Laboratory Operations & Safety Protocol 16 of 65 V1:2010 . File injury/accident reports with the secretary of the Chemistry Department. to the building manager or lab technician so that the equipment can be recharged of replaced. 1. Accident reports (see Appendix A. Keep the victim warm. apoplexy. Only give inhalation (aromatic spirits of ammonia or amyl nitrate) in the mouth only if the victim is unconscious.
This set-up is also based on as sense of trust that each one will do his share in ensuring these practices. It has spared us from serious theft because security measures were taken in earnest and implemented. William J. and its long tradition of high standards in safety. you will see that the good of many outweighs individual convenience. finances. are maintained to a high level of compliance.Chapter 3 The Use of Building & Facilities 3. Over the years. The building is named after Fr. While in some cases some convenience is sacrificed. who was instrumental in its design and construction. Schmitt. with a laboratory extension for undergraduate labs on the 2nd floor of the 3rd wing of the Science Education Complex (SEC-C). It has given us the edge on effective equipment maintenance because malfunctions were reported at once. and effort—has led to the conservation of resources. the Ateneo Chemistry Department has been able to maintain a good and effective working set-up which has yielded beneficial outcomes. The judicious use of resources—energy. Laboratory Operations & Safety Protocol 17 of 65 V1:2010 . The heart of an effective working set-up is the responsibility and discipline of users of the building and its facilities.1 Schmitt Hall T he Chemistry Department is housed at Schmitt Hall (formerly the Chemistry Building). which in the past have served the Department well. It has saved the Department from serious accidents because of the emphasis on safety at the workplace.
General Chemistry Lab Organic Chemistry Lab Analytical Chemistry Lab Physical Chemistry Lab. f. g. c. Offices a. e. d.3. 2nd Floor SEC-C 307 309 6. b. Director’s Office Staff Office GC-MS Lab NMR Lab 102 104 103 105 5. 2nd Floor SEC-C 209. Research Laboratories a. 205 2. National Chemistry Instrumentation Center (NCIC) a. d. d. f. 301 201 109. Department Office Faculty Offices Faculty Lounge 112 101. b. d. Lecture/Seminar Rooms 3. Stockroom Balance Room Instrumentation Room AFM Room Multimedia Room Glassblowing Workshop 208 308 310 204 111 4th Floor 4. c.2 Room Addresses 1.115. c. b. 115. e.116. b. Organic Synthesis Lab Natural Products Lab NCIC Wet Lab Biochemistry Research Lab Polymer and Materials Lab Polymer/Biochemistry Wet Lab Electrochemistry Lab 203 202 206 304 302 305 303 Laboratory Operations & Safety Protocol 18 of 65 V1:2010 . also houses the AAS and HPLC 207. Service Rooms a. Teaching Laboratories a. b. c. c.
to ensure the safety of lab workers.). 2. If an experiment requires longer than 15 hours. and doors. glasses. faculty and staff can stay inside the building until 10:00 pm. d. endorsed by the mentor/supervisor.4 Access Keys 1. At least two people should stay for overnight work. b.m. Lights should be turned on only in the appropriate areas of work. d. the opening and locking of rooms is the main responsibility of the faculty handling the class. 3. a. He.. ovens and other lab equipment (unless overnight run is explicitly allowed by senior faculty). plates. but both the lender and Laboratory Operations & Safety Protocol 19 of 65 V1:2010 . polymer and materials. Anyone staying beyond 10:00 p. Sign at the Night Log.m.3. their respective offices.1). Sink should be properly cleaned. should be cleaned. 2. coffee maker. Researchers and graduate students may loan keys to the special labs (instrumentation. should be responsible for obtaining permission and getting access to the main door. N2. During these times all doors should be unlocked for safety. they have the responsibility of seeing to it that the labs used are properly locked after use. b. Try to work out a schedule that will go from 6:00 a. etc. should be submitted to the secretary of the Chemistry Department at least 2 days before the event. gas lines (LPG. Never assume that someone else will do these for you. and the sub-master key (for labs). Food supplies should be sealed and arranged properly. c. and the Office of Administrative Services must be informed.m. 3. A written request form (Appendix A.m. The building is opened to the public by the building manager by 7:00 a. The last person to leave a laboratory should make sure that the following are closed/locked/turned-off properly: windows. 4. For research assistants and students doing research. overnight stay needs to be approved by the mentor and department chair. Anyone sleeping in the faculty lounge should be up by 6:30 a. b. However.m. Overnight stays in the building should be processed with the Department office: a. For classes. overnight stays are discouraged. natural products labs) from a faculty member. As a rule. etc. e. hoods. so that the entry or exit from the lab is easy in an emergency. Tables should be wiped clean. faucets. a. c. aircon. b. and locked by the utility man at 6:00 p. In case the building manager is not yet around by this time.3 Use of the Building 1. to 10:00 p. Toilet and shower room should always be maintained in a clean. lights. Bathroom tissue should not be removed from the toilet. All used utensils. The faculty lounge should be kept clean and orderly. It is for your own safety. a. This will allow the office to inform the administration and security. e. Only faculty members and the lab technicians have access to keys for the main door. Always lock doors after every use. 5. respectable condition. It is a basic rule in any good chemistry lab to have at least 2 people in the lab. Laboratories should only be unlocked when there are lab classes or when an experiment has to be performed in that lab. c.m. faculty or staff may open the main door and the classrooms.
These cannot be moved/relocated without the explicit approval of the Department Chair. and shut-down routines. A student cannot use the instrument alone unless he/she is authorized to use that instrument. Verify the power requirements of the instruments (110 or 220V) before plugging it into the outlet and turning it on. Keys should never be left lying around in the rooms. SHORT NOTES are provided as supplement to the manuals. 6. the research labs.)All users must sign the instrument logbook. indicating the time they are returning. especially the power-up. 10-15 min). If no sign is placed or if the time is over. When using the polymer and materials lab. This assumes responsibility in the use of the keys. they should take the responsibility of informing the faculty involved or the stockroom technicians when they are finished.the one who borrowed are accountable for the keys. 3. 3. The performance of the balances are dependent on temperature. Return the items to where they were found. 3. Access/use of any instrument requires check-in and authorization by faculty-in-charge. 3. they have to put up a sign on the door. Creating duplicates on your own is strictly prohibited. the physical chemistry lab.). Do not attempt to fix the equipment yourself. anyone with a key to the room should lock it and report the matter to the Department office. and AFM room. Report immediately to the person-in-charge or to the Department office when instrument supplies are running low (e. the AFM room. When rooms are unoccupied.g. and there are no other people using the room. b. but keep them closed. c. Unlock the doors. chart paper. 5.6 Use of Instruments 1. If they are going to leave the room for a short period of time (e. Laboratory Operations & Safety Protocol 20 of 65 V1:2010 . no equipment (major nor minor) can be taken out of the building. Do not unplug them from their voltage regulators. Understand the instrument thoroughly. either or both the aircon or the dehumidifier must be turned on (for the FTIR and the AFM). turn on the aircon. Faculty offices should never be left unlocked. Equipment and accessories used should be kept in proper order. etc. Please be considerate of faculty members who hold offices in some of these special labs. 4. without explicit approval of the Chairperson and a senior faculty member. Any loss of keys should be reported immediately to the Department office. This is needed to prevent further damage and to ensure speedy repair. 3. Report any noticeable malfunction of the instrument immediately by reporting it to the Department office. Most major instruments have voltage regulators. When using the balance room. (The student’s name must be marked in the Instrument Authorization Table posted in the analytical chemistry bulletin board. As a rule. warm-up. so that no fatal mistakes are committed. 2. and glassblowing workshop: a. Keep the doors locked even when leaving the room for a short while.g. If students are using the room and do not have easy access to the key. All users must familiarize themselves with the operation of the instrument. 2. ink.5 Special Rooms 1. 4. 4. Review the instructions before the operation. Access keys are loaned to you in trust. When using the instrumentation room. they should be locked at all times.
The student and faculty-in-charge schedules the next session. instrument use has priority over other use. date. Procedure for instrument check-out 1. Only students who passed the safety exam will be allowed to use the instrument. students who wish to use it must request the technician or a faculty member to open the lab for them. AAS and IR spectrophotometers). Equipment that is left on without any note will be shutdown after 6:00 pm. If someone is using it. a general check-in will be scheduled every July of each year. During the check-in. 2. If the instrument room is locked. analysis. For the commonly-used instruments (UV-Vis. He/She can get a copy of the form from the instrument manager. Instrument Parts c. he/she can ask a person who is already authorized to use the instrument to analyze his/her samples.7. oven. Safety and Precautionary Measures d. The student submits to the instrument manager for filing and documentation the instrument authorization form filled up to the certificate of authorization part. the student must schedule an instrument check-out with the faculty-in-charge. 5. 9. ON TH SPOT checking-in is not allowed. 8. The student who wishes to use the instrument must fill-up the instrument authorization form (Form A. comments) if you are leaving something (e. Consumables d. Working environment 3. Leave a note (name. do proper shutdown. Instrument accessories c. the faculty-in-charge must discuss the following to the student: a. instrument. The faculty-in-charge inspects the following during check-out: a. Only students who will be using the instrument more frequently will be checked-in.g. 2. time. If the student will be using the instrument only once or twice. 10. 3. Important Reminders and Considerations 4. The student’s mentor must approve the student’s plan to check-in.3). Lock the lab door immediately. The date of authorization must be different from the date of check-in. When done. Instrument General Working Principles b. After the last use of the instrument. Instrument parts b. Procedure for Instrument Check-in 1. For computers connected to the instrument. The student schedules the instrument check-in wth the faculty-in-charge one week before the actual check-in. The Instrument manager updates his list of students authorized to use the instrument. computer) turned on. The Faculty-in-charge signs the certificate of authorization if the student has demonstrated competency in using the instrument during the next sessions. The faculty-in-charge signs the check-in part of the instrument authorization form as proof of check-in. All graduating students must make sure that they have checked-out of the instruments they are authorized to use for clearance purposes. Short Notes of the Procedure e. 6. make sure he/she understands that you are leaving and you are turning over the responsibility of shutting down to him/her. Laboratory Operations & Safety Protocol 21 of 65 V1:2010 . water bath. The student must already have actual samples to run during check-in.
2. Mentor will be notified.Penalties Any student who fails to follow the policies and guidelines will be subject to the following penalties: First Offense: Oral reprimand from the instrument manager and the department chair. the department chairperson discusses with the requesting party the department policies on the use of instrument and the charges: a. The person writes a letter addressed to the department chairperson requesting to use an instrument. feasibility of the analysis and availability of the needed accessories. If the requesting party agreed to the terms and conditions. If the request is granted. Mentor will do necessary action. He/She must include as an attachment the details of the procedure and instrument conditions. Operator fee 4. the department chairperson assigns to the available faculty-in-charge the granted requests. Third Offense: One week ban in using the laboratory. 3. He/She must include in the letter the purpose of the instrument use. Procedure for instrument use by outside parties 1. The department chairperson together with the faculty-in-charge and/or the instrument manager decides whether to grant the request or not based on the instrument schedule. Mentor will be notified. Second Offense: Oral reprimand and community service. Laboratory Operations & Safety Protocol 22 of 65 V1:2010 . Instrument use and b.
emergency equipment. drink. eyewash stations. Drinking. unless it is explicitly allowed by the Chair and senior faculty. and cosmetics is a potential route for exposure to toxic substances.1 Overnight Operations If laboratory operations are carried continuously or overnight. biological hazards. 4. Temporary holding containers should be clearly marked. Never leave a set-up unattended overnight. etc. Lab refrigerators. The supervisor has the responsibility for determining whether the work requires special safety precautions. smoking materials. such as having two persons in the same room during a particular operation. are likewise not to be used for food storage.and hazard-sign systems in the lab should enable a person unfamiliar with the usual routine of the lab to escape in a emergency (or help combat it. handled. Work areas are to be kept clean. soft drinks.Chapter 4 General Safety Principles 4. 4. Other signs should be posted to show the locations of safety showers. and inert gas. security guards may be asked to check on the lab worker. snacks and lunches are not to be brought into laboratory areas. arrangements should be made between individuals working in separate laboratories outside working hours to crosscheck periodically. and consumed in an area free of hazardous substances. Cleanup should follow the completion of any operation of at the end of each day. chemicals should not be brought into designated eating areas. and fire extinguishers. and plans should be made to avoid hazards in case of failure. The safety. it is essential to plan for interruptions in utility services such as electricity. 4. laser operations. Chemicals that are no longer needed should not be permitted to accumulate in the lab. Laboratory Operations & Safety Protocol 23 of 65 V1:2010 . and Smoking Contamination of food. ice chests. Extinguishers are to be labeled to show the type of fire for which they are intended. Operations should be designed to be safe. Experiments known to be hazardous should not be undertaken by a worker who is alone in a laboratory.5 Warning Signs and Labels Laboratory areas that have special or unusual hazards must be posted with warning signs. Likewise. if appropriate). such as fire hazards (See Figure 8). Wastes are to be deposited in appropriately labeled receptacles. Standard signs and symbols have been established for a number of special situations. and controls. Access to exits.3 Eating. the laboratory lights should be left on and an appropriate sign should be placed on the door or near the set-up. Alternatively. In any case. Food should be stored. exits. and cold rooms. Glassware and utensils that have been used for lab operation are not to be used for food or beverages. When housekeeping standards fall. Stairways and hallways should not be used as storage areas. and chemicals and equipment must be properly labeled and stored. must be maintained free from obstructions. Waste containers must be labeled for the type of waste for which they are intended. water. Under normal working conditions. 4.4 Housekeeping There is a definite relationship between safety performance and orderliness in the laboratory.2 Working Alone Generally. it is prudent to avoid working in the laboratory alone. Coffee. safety performance inevitably deteriorates.
4. Pressure regulators and gauges must be compatible with the cylinder valves. may result in the rapid and violent oxidation of normally non-reactive materials. Gas cylinders must have the valve protection cover in place (see Figure 9 in the Appendix) except when in use. If the reaction cannot be opened directly to the air. Whenever a reaction is attempted for the first time (small quantities of reactants should be used to minimize the hazards). graphite. For movement within shops and laboratories. due to the oil residue. Shields are to be placed so that all personnel in the area are protected from hazard. even though the cylinders are secured to a dolly. 7..8 Systems Under Pressure Reactions should never be carried out in. chains or belts anchored to walls or benches (see Figure 9 in the Appendix). elevated temperatures and/or pressures. pipes. Although oxygen is quite safe under normal temperatures and pressures. A cylinder connected to a piece of equipment and properly supported is considered to be in use. Whenever operations are carried out under non-ambient conditions. Laboratory Operations & Safety Protocol 24 of 65 V1:2010 . Toxic and poisonous gases must be used only in fume hoods or other enclosures vented directly outdoors.g. 5. an inert gas purge and bubbler system should be used to avoid pressure build up. Gas cylinders must be used in an upright position and clamped securely at all times. or contamination. cylinders weighing less than 50 lbs may be carried. rolling. 5-10 times more material).7 Compressed Gases The following rules summarize a few of the basic guidelines for the use and storage of compressed gases: 1. gauges. Smoking is not permitted in the area where flammable gases are used or stored. 4. Acceptable methods of support include: wall-mounted or bench-mounted gas cylinder brackets. 6. Moving by spinning. Pressurized apparatus should have an appropriate relief device. 4. Whenever a familiar reaction is carried out on a larger than usual scale (e. a regulator for oil-pumped nitrogen could produce a serious explosion if subsequently used for oxygen. 2. regulators. For example. 8. etc. an apparatus that is a closed system unless it is designed and tested to withstand pressure. and fittings must be scrupulously free of oil. is prohibited. if desired. Appropriate dollies are to be used to move cylinders weighing more than 50 lbs. or any other oxidizable substance. and 3. Appropriate first aid and antidote information and supplies must be provided and clearly marked at room entrances. sliding.e. 2. i. whether full or empty. grease. 4. nor heat applied to.6 Shielding for Safety Safety shielding is to be used for any operation having the potential for explosion such as 1. 3. The pressure regulators must be removed and valve protection covers replaced before moving cylinders. All oxygen valves. the use of adapters is prohibited. Compressed gas cylinders must be supported and firmly restrained at all times.
Dry ice should be added slowly to a liquid portion of the cooling bath to avoid foaming over. Do not store in a refrigerator or freezer. Only glassware designed for vacuum work should be used for that purpose. The use of plastic or metal connectors should be considered. Insulated gloves and a face shield may be needed when preparing or using some cold baths. Tubing should be fire polished or rounded and lubricated. Workers should avoid lowering their head into a dry ice chest: carbon dioxide is heavier than air.) Broken glassware should be disposed of in a special container marked BROKEN GLASS.9 Cold Traps and Cryogenic Hazards The primary hazard of cryogenic materials is their extreme coldness.10 Glassware Accidents involving glassware are a leading cause of lab injuries. (For example. (Small pieces should be swept up with a brush into a dustpan. Wetting the glassware should also facilitate the process of inserting glass tubing into rubber stoppers. Adequate hand protection should be used when inserting glass tubing into rubber stoppers or corks or when placing rubber tubing on glass hose connections. and hands should be held close together to limit movement of glass should fracture occur.4. Careful handling and storage procedures should be used to avoid breaking glassware. Proper instruction should be provided in the use of glass equipment designed for specialized tasks. Glass-blowing operations should not be attempted unless proper annealing facilities are available. Vacuum-jacketed glass apparatus should be handled with extreme care to prevent implosions. which leads to an explosion hazard. Hand protection should be used when picking up broken glass. separatory funnels containing volatile solvents can develop considerable pressure during use. Equipment such as Dewar flasks should be taped or shielded. which can represent unusual risks for the first-time user. Neither liquid nitrogen nor liquid air should be used to cool a flammable mixture in the presence of air because oxygen can condense from the air. They. Laboratory Operations & Safety Protocol 25 of 65 V1:2010 . and suffocation can result.) Glassware which is to be heated should be Pyrex or a similar heat-treated type. can cause severe burns if allowed to contact the skin. 4. and surfaces they cool. Appropriate insulated gloves should be used when handling dry ice.
Open Office Spreadsheet or any text-capable reader 6. PDEA and PNP. Level 1 users may: 1. chemical expiration. Features 1. stock-level. students) No accounts are assigned to Level 1 users.e classification of wastes. Check availability of chemicals in the department 2.edu). efficient purchasing. Tracking of controlled chemicals under the EMB. which provide quick access to: ü Physico-chemical information ü Handling and storage conditions ü Health and safety information ü Disposal considerations 4. generation of reports (i. Reports are easily prepared using the system’s Reporting Service 5. chemical movement) can be exported as comma-separated version (CSV) files which can be opened in Microsoft Excel. threshold reports. Safety Data Sheets (SDS) bank Safety Data Sheets (SDS) for chemicals are stored in the system. Exportable Reports Reports (i. Each chemical bottle is provided with a tear. accurate inventory.and chemical resistant barcode sticker. Access to the different features depends on the user-levels as shown in below: User Level 1 (Guests. Back-up and Recovery Mechanisms CMIS has features which provide means to back-up the database and restore all data should the system fail 7. Different user-levels Only selected accounts can access restricted areas of the system. CMIS is meant to address the need for accessibility of information. management and compliance with various laws. 2. Information database Supplier and chemical information are stored in the database. The system may be accessed by any computer within the campus (http://cmis. Supplier details and hazard codes) Laboratory Operations & Safety Protocol 26 of 65 V1:2010 . Obtain chemical information on chemicals (i.1 Chemical Management and Inventory System (CMIS) The Chemical Management and Inventory System (CMIS) combines a web-based inventory system and barcode scanner to provide a means for efficient updating and query of chemical stock levels. CAS number.Chapter 5 Chemical Management 5. Scanner-server synchronization Chemical data in the barcode scanner are easily synchronized with data in the server 8. The system of tracking is container-based. PDEA and PNP System not only allows monitoring of chemicals on-stock but also controlled chemicals under the EMB.ateneo.e. IUPAC name. chemical waste disposal and chemical waste movement) User Levels CMIS has four (4) user-levels (Table 1). Hazardous Waste Inventory System The CMIS is linked to the Hazardous Waste Inventory System (HWIS) which allows monitoring of chemical waste generation and disposal. 3. Using the CMIS. consumption.e.
Any special instructions for this particular chemical? – Special instruction on how the chemical is to be handled or stored should be noted here. click on the “Click here to View Existing Chemical Request” 2. click on Stock Inquiry. Checking status of request 1. Requests for other chemicals can be added to current request by clicking on “Click here to add more chemical requests. Research Assistants (RAs) and junior faculty) An account and temporary password is given to Level 2 users. print the form and have it signed by your thesis mentor. To check for stock levels of chemicals using the Inventory System. Proceed by clicking on the “Click Here to Request for a Chemical” link. Laboratory Operations & Safety Protocol 27 of 65 V1:2010 . Access (Material) Safety Data Sheets ((M)SDSs) of chemicals 4. Users may change the password using the Password Management feature of the system. 3. Input the reference number issued upon checked out from the Request Wizard. The system will then ask for the chemical’s common name. In addition to the features available to a level 1 user. Provide the chemical’s common name. If request has been approved. Fill up the form with the required information to proceed. Stock Inquiry 1. Level 2 users also have access to information regarding the stock level and stock location of the chemicals.” 7. The system will then provide the requestor with an update on the status of his/her request. To end request wizard. Please take me to check out. the system will then ask for the following: Concentration – what is the concentration of the chemical that you need? Grade – what is the grade of the chemical? How much do you need? – Input amount is in liters or grams. List of requested items may also be viewed by clicking on “Click here to review requested items. click on “I do not need any more items. Requestor Name – the name of the requesting party (who made the request?) Requestor Department / Company – the department or company affiliation of the requestor Purpose – the reason for the request (where will the chemical be used for?) Request Date – date of the request (when was the request made?) 4.” 8. Click on the Chemical’s Common Name link to show the information about the chemical. Show the signed form to the stockroom personnel and claim chemicals. To Request for Chemicals. A page will appear where the Chemical Name or IUPAC Name or CAS Number of the chemical can be entered. The Request Home Page will then be shown. 5. 4. To check the status of chemical requests.” The check-out page will then show the reference number which can used by the requestor to review status of request. If the chemical is on stock. 2. click on the “Request” Link from the Home Page. User Level 2 (Teaching Assistants (TAs). 3. Request for a chemical Steps in requesting for a chemical (See Figure 11 in the Appendix for the overview) 1. 2. 3. 6.3.
This includes management of CMIS accounts. To save the back-up file. 5. and disposal of chemicals. Level 4 users have access to all of the features of the CMIS. storage and disposal of the following controlled chemicals: Laboratory Operations & Safety Protocol 28 of 65 V1:2010 . encode additional data (e. Lab technicians. Suppliers. hazard codes and threshold levels may be done by clicking on the appropriate links (i. right-click on the “The Back-up file” link and choose “Save Link As…” or “save Target As…” Synchronization of server and barcode scanner The sync station software is used to synchronize the barcode reader’s database with the server’s database. consumption. thresholds etc. Senior Faculty) An account and temporary password is also given to Level 3 users. In addition to the features available to a level 3 user. 4. 5. Click on the “Synchronize All” button to perform all operations in sequence. storage locations. storage. User Level 4 (Dean.User Level 3 (Department secretary. Sub-pages for the selected field will then be displayed.e. Laboratory managers. In addition to the features available to a level 2 user. CMIS manager. chemical grade. The system will then display a dialog box once all operations are finished.e. Different implementing agencies (i. add/update chemical bottle inputs. Place the scanner in the dock and wait for “active sync” to finish synchronization. click on the link ”Back-up and Recovery” link on the Administrator Functions Section.). dangerous drugs and explosives (Tables 2 and 3 in the Appendix). Setting up of additional data such as supplier information. chemical brand. Users may change the password using the Password Management feature of the system. preparation of a back-up of the database and synchronization of the server and barcode scanner. movement. click on the “add ____” link to display the form where new entries can be supplied. 1.” button to submit and save new data. the government has started to implement various laws. Open the Sync Station software and ensure that all databases are connected. To back-up the databse. 3. The safety officer together with the system administrator of the MIS office is in-charge of the maintenance of the database system. chemicals and thresholds) on the home page. Department Chair. Level 4 users also have access to the hazardous waste inventory system and the administrative functions of the system. Setting up of additional data to the CMIS 1. Level 3 users also have access to the reporting services of the system. rules and regulations covering a broad range of activities related to the purchase.g. Then click the link. Preparing a back-up of the database 1. “Back-up. To synchronize the server and barcode scanner.2 Controlled Chemicals In 2004. new storage locations. close all active application in the barcode scanner.” 2. storage locations. technicianin-charge of CMIS) An account and temporary password is also given to Level 4 users. use. Users may change the password using the Password Management feature of the system. Click “add ____. These can be grouped under the general headings of environment. A “Back-up succeeded” message will then appear on the screen. supplier information. chemical brands. PNP and PDEA) monitor the purchase. 2. 2. waste management. EMB. hazard codes. To add new data.
Requesting party may then pick-up the requested chemicals from the stockroom. 2. 3. The person checks availability of chemical using the CMIS. sale. This license is renewed yearly. administration. manufacture. sale. If the request is granted.EMB The EMB regulates mercury. Since these chemicals are also used in the teaching laboratories. administration. The department chairperson together with the faculty-in-charge and/or the person in-charge of controlled chemicals decides whether to grant the request or not based on the amount of chemical on stock. delivery. possession or use of explosives and explosive ingredients such as nitrates and chlorates. The university is required to register its possession and use of Mercury and Cyanide compounds. A separate “Permit to Purchase and Move Explosive and Explosive Ingredients” should also be obtained from PNP prior to purchase of nitrates and chlorates. delivery. distribution. Monthly consumption reports are also submitted to PNP. upon presenting the official receipt from the Cashier. PDEA PDEA monitors and regulates the importation. The university is required to obtain a Purchaser’s license from PDEA. Procedure for requesting for controlled chemicals by outside parties 1. the department chairperson approves the request. 4. the person then writes a letter addressed to the department chairperson requesting to purchase the chemical. from their importation. PNP PNP monitors and regulates the importation. If the chemical is available. Since the department uses these compounds for laboratory classes and research. transport and disposal. transportation. Semiannual reports on the purchase and consumption of PDEA-controlled chemicals are also submitted to PDEA. Requesting party proceeds to the Cashier to pay for the chemicals being purchased. use. He/She must include in the letter where the chemical will be used as well as the amount of the chemical being requested. cyanide and their compounds. transportation. distribution. Laboratory Operations & Safety Protocol 29 of 65 V1:2010 . possession or use of the following prohibited and regulated drugs and precursor chemicals (See Table 4 in the Appendix) Since the department uses some of these compounds for laboratory classes and research. the university is required to obtain a “License to Possess Explosive and Explosive Ingredients” from PNP.
toxic or highly toxic agents. or mucous membranes. hepatotoxins. Sensitizer: a chemical that causes a substantial proportion of exposed people or animals to develop an allergic reaction in normal tissue after repeated exposure to the chemical. affecting one or more organs. (c) a chemical that has a median lethal concentration (LC50) in air of 200 ppm by volume or less of gas or vapor. irritants. whether by initiating or promoting it. Local toxicity refers to the direct action of chemicals at the point of contact. such as chloroform. Chemicals can have local or systemic effects. 5. liver enlargement. living tissue by chemical action at the site of contact. Irritant: a chemical. fume.Chapter 6 Handling Chemicals 6. Some of these compounds are fairly common materials used in many laboratories. thiourea. or irreversible alterations in.000 mg/kg body weight when administered by continuous contact for 24 hrs (or less if death occurs within 24 hrs) with the bare skin of albino rabbits weighing between 2-3 kg each. 4. 6. when administered by continuous inhalation for one hour (or less if death occurs within one hour) to albino rats weighing between 200-300 g each. Signs & symptoms: jaundice. or dust. Acute effects are observed shortly after exposure.000 ppm by volume or less of gas or vapor. reproductive toxins. fume. and agents which damage the lungs. 2. Carcinogen: a chemical agent that causes a malignant disease or increases statistically the risk of cancer. It is therefore wise to minimize exposure to chemicals. 1. (b) a chemical that has a medial lethal dose (LD50) of 200 mg/kg but not more than 1. nephrotoxins. corrosives. Toxic effects are also classified as acute or chronic. (c) a chemical that has a median lethal concentration (LC50) in air of 200 ppm but not more than 2. nitrosamines. but causes reversible inflammatory effect on living tissue by chemical action at the site of contact. sensitizers. It includes chemicals which are carcinogens. which is not corrosive. skin. or 2 mg/L or less of mist. Chronic effects result from long-term exposure or appear after a latency period.1 Hazardous Chemicals All chemicals have toxic effects at some dose level for some route of exposure. or 2 mg/L or less of mist. (b) a chemical that has a medial lethal dose (LD50) of 200 mg/kg body weight when administered by continuous contact for 24 hrs (or less if death occurs within 24 hrs) with the bare skin of albino rabbits weighing between 2-3 kg each. Health hazard means a chemical for which there is statistically significant evidence based on at least one study conducted in accordance with established scientific principles that acute or chronic health effects may occur in exposed personnel. or dust. benzene. Highly toxic: (a) a chemical that has a median lethal dose (LD50) of 50 mg/kg body weight when administered orally to albino rats weighing 200-300 g each. eyes. hydrazine. 3. 7. carbon tetrachloride. Corrosive: a chemical that causes visible destruction of. Systemic toxicity occurs when the chemical agent is absorbed into the bloodstream and distributed throughout the body. when administered by continuous inhalation for one hour (or less if death occurs within one hour) to albino rats weighing between 200-300 g each. Toxic: (a) a chemical that has a median lethal dose (LD50) of 50 mg/kg but not more than 500 mg/kg body weight when administered orally to albino rats weighing 200-300 g each. benzene Laboratory Operations & Safety Protocol 30 of 65 V1:2010 . Targeted Organ Effects a. Hepatotoxins: chemicals which produces liver damage. and o-toluidine. dioxane. Hazardous chemical means any chemical which is capable of causing harm to people and the environment. neurotoxins. agents which act on the hematopoietic system. Chemicals: carbon tetrachloride.
4. explosive. d. loss of consciousness. Signs and symptoms: edema. Signs & symptoms: cough. Note: flashpoint means the minimum temperature at which a liquid gives off a vapor in sufficient concentration to ignite when tested according to ASTM D 56-79. flammable. except any mixture having components with flashpoints of 37.8 oC. gas. regardless of the lower limit.2 oC. in a container. flammable: an aerosol that. when tested by the method described in 16 CFR b. but below 93. Gas. Liquid. Chemicals: lead. chlorinated compounds. decrease in motor functions. asbestos. Chemicals: ketones. uranium. that is liable to cause fire through friction. corneal damage. regardless of the pressure at 21. the total of which make up 99% or more of the total volume of the mixture. forms a flammable mixture with air at a concentration of 13% by volume or less. pressure. at ambient pressure and temperature.8oC as determined by ASTM D-323-72. or (b) a gas that.8 oC or higher. or which can be ignited readily and when ignited burns vigorously and persistently as to create a serious hazard. e. 2. pyrophoric. tightness of chest. or retained heat from manufacturing or processing. Flammable: a chemical that falls into the following categories: a. flammable: any liquid having a flashpoint below 37.45.3 oC. proteinuria. carbon disulfide. or high temperature. Signs & symptoms: conjunctivitis. A chemical shall be considered to be a flammable solid if. or higher. Reproductive toxins: chemicals which affect the reproductive capabilities including chromosomal damage (mutations). unstable (reactive) or water-reactive. g. sterility. d. almost instantaneous release of pressure. shortness of breath. cyanides. 1. Signs & symptoms: narcosis.3 oC. at an ambient temperature and pressure. and effects on fetuses (teratogenesis). Laboratory Operations & Safety Protocol 31 of 65 V1:2010 .4 oC. Chemicals: organic solvents. Neurotoxins: chemicals which produce their primary toxic effect on the nervous system. Cutaneous hazards: chemicals which affect the dermal layer of the body. forms a range of flammable mixtures with air wider than 12% by volume. the total volume of which make up 99% or more of the total volume of the mixture. absorption of moisture. and ASTM D 3278-78. flammable: (a) a gas that.8 oC. or (c) a liquid having a vapor pressure exceeding 40 psi at 37. Signs & symptoms: defatting of the skin. and heat when subjected to sudden shock. 3. an organic peroxide. h. Combustible liquid: any liquid having a flashpoint at or above 37. Signs & symptoms: cyanosis. when tested by the method described in 16 CFR 1500. an absolute pressure exceeding 40 psi at 21. (b) a gas or mixture of gases having. Eye hazards: chemicals which affect the eye or visual capacity. yields a flame projection exceeding 18 inches at full valve opening. Agents which damage the lung: chemicals which irritate or damage pulmonary tissue. f. Compressed gas: (a) a gas or mixture of gases having. Signs & symptoms: birth defects. in a container. Solid flammable: a solid other than a blasting agent or explosive. spontaneous chemical change. Chemicals: halogenated hydrocarbons. a compressed gas.1 oC. Aerosol.b. deprive the body tissues of oxygen. ASTM D 93-79. except any mixture having components with flashpoints of 93. c. Nephrotoxins: chemicals which produce kidney damage. behavioral changes. Chemicals: mercury. c. DBCP. an absolute pressure exceeding 104 psi at 54. an oxidizer. Explosive: a chemical that causes sudden. and irritation. rashes. Chemicals: carbon monoxide. Chemicals: silica. Physical hazard means a chemical for which there is scientifically valid evidence that it is a combustible liquid. acids. Agents which act on the blood or hematopoietic system: decreases hemoglobin function. or a flashback (a flame extending back to the valve) at any degree of valve opening.
Inhalation of toxic vapors. sebaceous glands. and lungs and can seriously damage these tissues. A common result of skin contact is localized irritation or dermatitis. or will become self-reactive under conditions of shocks. or as produced or transported. where one or both of the hydrogen atoms has been replaced by an organic radical. its solubility in tissue fluids. Alert laboratory personnel will take care not to create unnecessary aerosols.44. Many of the particulates generated during such procedures do not settle out but remain suspended in the air and are carried about by air currents in the room. However. throat. and the duration of exposure. decompose. 3. 7. stirring. by local action. and cuts or abrasions of the outer layers of the skin. a number of materials are absorbed through the skin to produce systemic poisoning. glassware. or cuts. Grinding and crushing procedures can also produce aerosols. mists. its concentration. Inhalation hazards are often associated with gases and volatile chemicals. Organic peroxide: an organic compound that contains the bivalent —O-O— structure. Laboratory Operations & Safety Protocol 32 of 65 V1:2010 . Laboratory chemicals in the form of dusts and particulates can become airborne when transferred from one container to another. laboratory personnel may unconsciously contaminate themselves when they touch work surfaces. 6. Pyrophoric: a chemical that will ignite spontaneously in air at a temperature of 54. will vigorously polymerize. eyes. all laboratory operations involving an open vessel will result in the release of an aerosol. it ignites and burns with a self-sustained flame at a rate greater than one-tenth of an inch per second along its major axis. Oxidizer: a chemical other than a blasting agent or explosive. nose. Some of these particulates are capable of being inhaled and deposited in the respiratory tract. that initiates or promotes combustion in other materials. One of the most frequent exposure to chemicals is by contact with the skin. 5. Inhaled gases or vapors may pass rapidly into the capillaries of the lungs and be carried into the circulatory system. Such operations include weighing. For many operations it is not obvious that an aerosol is being generated and laboratory personnel may not be aware that a hazardous situation exist.2 Routes of Exposure Dermal Contact. Unstable (reactive): a chemical which in the pure state. 9. Splash created from spills and during vigorous shaking and mixing also results in aerosol formation. 2. Also. sores. Inhalation. which facilitate the absorption of chemicals into the body. pipeting. sweat glands. The main portals of entry for chemicals through the skin are the hair follicles. 8. condense. injections with a needle and syringe. thereby causing fire either of itself or through the release of oxygen of other gases. Water reactive: a chemical that reacts with water to release a gas that is either flammable or presents a health hazard.1500. but solids and non-volatile liquids can also present an inhalation hazard for laboratory personnel. Spills and splash can result in overt contamination of the skin. pouring. The follicles and glands are supplied with blood vessels. Chemicals can also gain entrance into the body when contaminated hands touch the mouth. and removing caps and stoppers. pressure or temperature. or equipment which become contaminated curing experimental activity. or dusts can produce poisoning by absorption through the mucous membrane of the mouth. 1.4 oC or below. 6. Actually. The degree of injury resulting from inhalation of toxic substances depends on the toxicity of the material. and which may be considered to be a structural derivative of hydrogen peroxide. gases.
such as lasers and ultraviolet light. Full-face shields that protect the face and throat should always be worn when maximum protection from flying particles and harmful liquids is needed. These should have: a minimum thickness of 3 mm. 1. 2. It is the responsibility of the lab supervisor to determine the level of eye protection required and to enforce eye-protection rules. and lens-retaining frames. “Contact lenses should not be worn in the chemistry laboratory Laboratory Operations & Safety Protocol 33 of 65 V1:2010 . Face Shields: Goggles offer little protection to the face and neck. For full protection. Eye and protective devices must be worn by students. Few substances are innocuous in contact with the eyes and a considerable number are capable of causing burns and provide for rapid absorption of many chemicals. containers of toxic chemicals may break resulting in hazard from contact with broken contaminated glass. Furthermore. For example. passed flammability tests. Splash goggles (“acid goggles”) that have splash –proof sides should be used when protection from harmful chemical splash is needed. The eyes are of particular concern because they are so sensitive to irritants. and visitors in laboratories where chemicals are stored or handled. A face shield or mask may be needed when a vacuum system (which may implode) is used or when a reaction that has a potential for mild explosions is conducted. more than two hours per day). Food items and utensils themselves can become contaminated when stored in the laboratory. Impact-protection goggles have screened areas on the sides to provide ventilation and reduce fogging of the lens and do not offer full protection against chemical splashes. The needle and syringe is one of the most hazardous items used in the laboratory. and lens diameter. or highly particulate atmosphere. Other eye protection should be worn when a significant splash hazard exists. safety glasses should be worn with face shields. intense heat. 3. There are specific goggles and masks for glassblowing. nose bridge width. The minimum acceptable eye protection requires the use of hardened-glass or plastic safety spectacles. and intense light sources. welding. Three dimensions that are important in providing a comfortable fit include: temple length. impact resistance. Contact Lenses: Contact lenses do not provide eye protection in the industrial sense. when the work environment entails exposure to chemical fumes. Goggles: Goggles are not intended for general use. Injection. Ocular exposure can occur via splash or when contaminated hands rub the eyes. The type of safety device required will depend on the nature of the hazard and the frequency with which it is encountered. Also. or splashes. staff. In addition. goggles should be worn when working with glassware under reduced or elevated pressure and when glass apparatus is used in combustion or other high-temperature operations.Ingestion. contact lens use should be restricted. identification of contact lens wearers should be ensured for appropriate emergency care and for protection in work areas hazardous to the eyes. and must be worn only in conjunction with approved safety eyewear. faculty. The American Chemical Society’s Committee on Chemical Safety states in Safety in Academic Chemistry Laboratories that. 6. They are intended for wear when there is danger of splashing chemicals or flying particles. Safety spectacles are recommended for those who require eye protection frequently and/or for long durations (e.3 Protective Clothing and Equipment Eye and Face Protection. Accidents involving needles and syringes can results in injection contamination through the skin. vapor. Side shields that attach to regular safety spectacles offer some protection from objects that approach from the side but do not provide adequate protection from splashes. Safety glasses: Ordinary prescription glasses do not provide adequate protection from injury to the eyes. Ingestion of toxic materials in the laboratory can also occur when contaminated hands come in contact with the mouth of with food items which are placed in the mouth.g. molten metals. Ocular Exposure. 4. The practice of mouth pipeting can result in aspiration of toxic materials.
If the possibility of chemical contamination exists. combustibility) to the wearer. if significantly contaminated by them. for inserting glass tubes into rubber stoppers. Such items must be removed promptly and decontaminated or discarded. aniline vapor. a known carcinogen. Use of Gloves. Loose or torn clothing and unrestrained long hair can easily catch fire. and tears. Plastic or rubber aprons provide better protection from corrosive or irritating liquids but can complicate injuries in the event of fire. Lab workers should know the appropriate techniques for removing protective apparel. should be removed immediately. however. belts) can be especially hazardous because many chemicals can be absorbed in the leather and then held close to the skin for long periods. which are self-contained breathing apparatuses with their own air tanks. although the wearer is protected against inhalation exposure. Laboratory Operations & Safety Protocol 34 of 65 V1:2010 . if the material is impermeable to water. they can be used safely for limited time periods if specific use and glove characteristics (i. Glove materials are eventually permeated by chemicals. Before each use. Common glove materials include neoprene. Finger rings can react with chemicals and also should be avoided around equipment that has moving parts. Insulated gloves should be used when working at temperature extremes. Such personnel should not wear loose. Chemical spills on leather clothing or accessories (watchbands. gloves should be inspected for discoloration. Furthermore. Respiratory Protection. the removal of the contact lens to achieve immediate irrigation is made nearly impossible by involuntary spasm of the eyelid… Gases and vapors can concentrate under the lenses and cause permanent eye damage. In that event. These materials differ in resistance to various substances. and synthetic materials such as Nomex or Tyvek are satisfactory. Leather gloves may be used for handling broken glassware. The clothing worn by lab workers can be important to their safety. personal clothing that will be worn home should be covered by protective apparel. sharp edged objects.except for therapeutic reasons… In the event of a chemical splash into an eye. Gloves should be selected on the basis of the material being handled. However. Laboratory coats and aprons are intended to prevent contact with dirt and the minor chemical splashes or spills encountered in lab-scale work. are primarily a protection for clothing. and very hot or very cold materials. rayon and polyesters are not. and may itself present a hazard (e. Various synthetic materials such as Nomex and Kevlar can be used briefly up to 538 oC. capillary action tends to hold the offending liquid under the contact lens and against the surface of the cornea. or torn clothing and unrestrained long hair. etc.g. For these reasons. thickness and permeation rate and time) are known. Proper gloves should be worn whenever the potential for contact with corrosive or toxic materials and materials of unknown toxicity. the particular hazard involved. and their suitability for the operation being conducted. Before removal. Furthermore. Coats and aprons do not significantly resist penetration by organic liquids and. polyvinyl chloride. Clothing and Protective Apparel. and for similar operations where protection from chemicals is not needed. Skimpy clothing offers little protection to the skin in the event of chemical splash. and butyl and natural rubbers. It is best not to use gloves made either entirely or partly of asbestos. Cotton (pure or 60:40 cotton-polyester). It is the responsibility of the lab supervisor to determine whether specialized hand protection is needed for any operation and to ensure that needed protection is available.e. plastic aprons can accumulate considerable static charge and should be avoided in areas where flammable solvents could be ignited. These. Contact lenses can also trap particulate foreign matter in the eye and thereby produce abrasion of the cornea. nitrile. only contact lenses worn for therapeutic reasons can be permitted in the laboratory. especially any that has become contaminated. or become ensnarled in apparatus and moving machinery. shoes. he is warned against absorption through the skin of such substances as HCN gas.” Masks may be used to minimize exposure to noxious fumes. punctures. skimpy. but they are by no means total respiratory protection as air masks. dip into chemicals. Double gloving is recommended when handling highly toxic or carcinogenic materials. gloves should be washed.
Placing a reacting chemical system within a hood. Shoes should be worn at all times in laboratories or other areas where chemicals are used or stored. The use of liquid soap dispensers is recommended. drinking. Work surfaces should be protected from contamination by using disposable plastic backed absorbent paper or stainless steel trays. 4. if dropped. which. application of cosmetics. fires. Chemical fume hoods provide personnel protection by means of directional airflow from the laboratory into the hood through the face opening. etc. This not only protects those people who might otherwise enter the lab. Mechanical pipeting aids are to be used for all pipeting procedures. Since all procedures involving an open vessel of liquids or powders generate aerosols. This barrier can afford workers protection from chemical splash. sprays. Minimizing Aerosols. sandals. 3. food. chewing of gum. Access to laboratories which have toxic chemicals should be controlled.Foot Protection. Other items and equipment which become contaminated during experimental activity should be decontaminated with the appropriate solvent. smoking. would likely result in a foot or toe injury. Chemical fume hoods are the primary containment device in the laboratory to control airborne contaminants generated during experimental procedures. 2. or allowing the contents to run down the wall of the receiving vessel. Dry sweeping or dry mopping contaminated laboratory floors could re-aerosolize contamination. These should be placed in properly-labeled waste containers for disposal. or against lacerations from sharp edges. Dropping the contents from a height generates greater aerosol. Discharge fluids from pipets as close as possible to the fluid level of the receiving vessel. Care should also be taken when discarding contaminated cloves or plastic backed absorbent paper used to cover the work surface so that contamination is not aerosolized in the process. 6. Personnel should wash their hands immediately after completion of any procedure involving toxic chemicals and when they leave the lab. Safety shoes are required for personnel whose job duties require the lifting. Laboratory Operations & Safety Protocol 35 of 65 V1:2010 . Eating. against crushing by rolling objects. The laboratory door should be kept closed while experiments are in progress. or cloth sneakers should not be worn in labs or areas where mechanical work is being done. Procedures involving volatile chemicals and those involving solids or liquids that may result in the generation of toxic aerosols should be conducted in a chemical fume hood rather than on the open bench. Floors should be cleaned with a wet mop or with a vacuum cleaner equipped with a HEPA filter. or food containers should not be allowed in labs where toxic materials are used. but also reduces interruptions to the lab worker which could lead to accidents. of objects weighing more than 15 lbs.4 Handling Toxic Materials Access Control. Perforated shoes. Safety shoes are used to protect the feet against injuries from heavy falling objects. Decontamination of Work Surfaces. Personnel Practices. or storage of utensils. places a physical barrier between the workers in the lab and the chemical reaction. This airflow reduces the potential for escape of airborne contaminants into the laboratory. Use of Chemical Fume Hoods. or moving. and minor explosions. Rapid mixing of liquids with pipets by alternate suction and expulsion or forcibly expelling material from a pipet should be avoided. carrying. the laboratory worker should develop techniques which will minimize the creation of aerosols: 1. especially with the hood sash closed. The practice of mouth pipeting should also not be allowed.
5 Handling Carcinogens Procedures for handling toxic materials also applies to chemical carcinogens. 5. it should be placed in a tightly-stoppered sample tube or otherwise safeguarded from contaminating the lab. Working Quantities. Stock quantities of chemical carcinogens are to be stored in designated storage areas. Disruptive room air currents should be minimized by avoiding traffic near fume hoods and opening and closing doors near fume hoods while experiments are in progress. including water aspirators. An inventory of stock quantities should be maintained. 4. Storage. Face velocities between 100 to 120 feet per minute are acceptable for most uses. Each vacuum service. 7. or be removed for subsequent disposal. 6. Hood work areas should be clear of unnecessary equipment and materials which can disrupt airflow and block vents. Work should be carried out as far back in the hood as possible. and their access controlled. Verify airflow PRIOR to doing an experiment by attaching a Kimwipe or ribbon to the sash. 3. vapors or aerosols produced by these instruments should be captured through local exhaust ventilation at the site of their production. all of the materials needed for a procedure are present in the hood to eliminate disruption of airflow by carrying equipment in and out during a procedure. Quantities of chemical carcinogens present in the work area should be kept to a minimum. The storage areas should be posted with signs bearing the legend: CAUTION – CHEMICAL CARCINOGEN Authorized Persons Only. Experiments should be planned so that. Quantities should not normally exceed the amounts required for use in one week. Storage vessels containing working quantities should be labeled: CAUTION – CHEMICAL CARCINOGEN. a separate vacuum pump or other device placed in an appropriate chemical fume hood should be used. Check efficiency of the hood by measuring its average face velocity regularly. Use of Analytical Instrumentation. as much as possible. and should include the dates of acquisition and disposition. The outer container is to be labeled both the name of the chemical carcinogen and the warning: CAUTION – CHEMICAL CARCINOGEN. and Identification. The procedures outlined in this section are additional precautions in dealing with carcinogenic materials. Storage vessels containing stock quantities should be labeled: CAUTION – CHEMICAL CARCINOGEN. Chemical carcinogens Laboratory Operations & Safety Protocol 36 of 65 V1:2010 . Use equipment with legs. when used with chemical carcinogens. 2. When this is impossible. are to be placed entirely within a chemical fume hood. Contaminated materials which are transferred from work areas to disposal areas must first be placed in a closed plastic bag or other suitable impermeable and sealed primary container. Analytical instruments. is to be protected with an absorbent or liquid trap and a HEPA filter to prevent entry of any chemical carcinogen into the system. The primary container must be placed in a durable outer container before being transported. Inventory. 6. Analytical equipment that becomes contaminated should not be used until it has been completely decontaminated. Contaminated materials must either be decontaminated by procedures that decompose the chemical carcinogen. When using a volatile carcinogen. When a sample is removed from the analytical instrument. Laboratory Transport. Protection of Vacuum Lines.The following practices should be observed when using fume hoods: 1. Storage vessels containing chemical carcinogens are to be first placed in an unbreakable outer container before being transported to lab work areas. Keep sash as low as possible. 8. Decontamination. Hoods should not be used for storage of chemicals.
When transferring flammable liquids in metal equipment. Prevention of fires and explosions requires knowledge of the flammability characteristics (limits of flammability.which have spilled out of a primary container so as to constitute a hazard must be inactivated in situ or should be absorbed by appropriate means for subsequent disposal. 6. Spontaneous Ignition or Combustion: this takes place when a substance reaches its ignition temperature without the application of external heat. 2. Flammable substance should be handled only in areas free of ignition sources. Diethyl ether (ignition temperature 160 oC) can be ignited by the surface of a hot plate. 3. This is of special concern when working in a dry environment such as an air-conditioned room or a room with a dehumidifier. When nonmetallic containers (plastic) are used. oil baths. A means for assuring adequacy of clean-up should be provided.6 Flammable Materials The ability to vaporize. 1. static electricity. For example: A steam line or a glowing light bulb may ignite carbon disulfide (ignition temperature 80 oC). and special note should be taken of ignition sources lower than that at which the substance is being used. 2. the bonding can be made to the liquid rather than to the container. The degree of the fire hazard also depends on the ability to form combustible or explosive mixtures with air. dust accumulations. The basic precautions for safe handling of flammable materials include the following: 1. ASTM D 93-79. This possibility should be recognized. Metal lines and vessels discharging flammable substances should be properly bonded and grounded to discharge static electricity. ignition requirements. heating mantles. When flammable materials are used in the lab. the flame will propagate itself from the point of ignition back to its source. it is the vapors from the liquid that burn. especially when materials are stored or disposed of. and hot air baths. and phosphorous. for instance wipe tests or fluorescence tests. A flammable liquid does not itself burn. close attention should be given to all potential sources of ignition in the vicinity. Many common laboratory solvents and chemicals have flashpoints that are lower than room temperature. Flammable vapors from massive sources such as spillages have been known to descend into stairwells and ignite on a lower story. and burn or to explode flammable materials varies with the specific type of substance. alkali metals such as sodium and potassium. peroxides. water baths. chlorates. Preferred head sources include steam baths. lighted matches. Flammable substances should never be heated by using an open flame. There are a number of potential sources of spark. and persulfates). organic materials mixed with strong oxidizing agents (such as nitric acid. The rate at which different liquids produce flammable vapors depends on their vapor pressure. finely divided pyrophoric metals. and ASTM D 3278-78. permanganates. The vapors of flammable liquids are heavier than air and capable of traveling considerable distances. or heat in labs which can ignite flammable substances: open flames. Flashpoint: the minimum temperature at which a liquid gives off a vapor in sufficient concentration to ignite when tested according to ASTM D 56-79. If the path of vapor with the flammable range is continuous. The possibility of spontaneous combustion should be considered. Ignition Temperature (Auto-ignition temperature): the minimum temperature required to initiate or cause self-sustained combustion independent of the heat source. Properties of Flammable Substances. flame. ignite. static-generated sparks should be avoided by bonding and use of ground straps. 3. Handling. and of the appropriate procedures top use in handling such substances. and hot surfaces. Sources of Ignition. and burning rates) of combustible materials likely to be encountered under various conditions of use. Laboratory Operations & Safety Protocol 37 of 65 V1:2010 . Materials susceptible to spontaneous combustion include oily rags.
2. Flammable liquids must be stored so that accidental contact with strong oxidizing agents (such as permanganates or chlorates) is avoided. Ventilation is one of the most effective ways to prevent the formation of flammable mixtures. and many plastics require water or dry chemical type extinguisher labeled A. cloth. Flammables stored in the open in the laboratory work area shall be kept to the minimum necessary for the work being done. 4. containing Halon 1211 or CO2. wood. A hazardous reaction occurs when two or more incompatible chemicals result in an undesirable or uncontrolled reaction with adverse consequences. fuse boxes. and. labels A. 3. For easy identification. or when they are unwittingly combined during experimental procedures. light. pictograms are used to indicate the type of fire on which an extinguisher is to be used. Fire extinguishers are labeled according to their fire fighting proficiency and safety in extinguishing various types of fires. solubilization of toxic substances. volatilization of toxic or flammable substances. When a flammable liquid is withdrawn from a drum. Halon. dispersal of toxic dusts/mists/particles. 3. They may result when incompatible chemicals are accidentally spilled. formation of shock or friction sensitive compounds. or D. formation of flammable gases. or CO2 extinguisher labeled C. Using the “wrong” type of extinguisher on a fire can endanger the user and make the fire worse. Metals: Combustible metals such as magnesium and sodium require special extinguishers labeled D. heated in open containers. An exhaust hood should be used whenever appreciable quantities of flammable substances are transferred from one container to another. 5. Electrical Equipment: Fires in wiring. Ordinary Combustibles: Fires in paper. offer first defense against flammable liquids or electrical fires without leaving a powder residue which could harm electronic equipment. Halon or CO2 extinguisher labeled B.7 Handling Explosive/Reactive Materials The large number of different classes of chemicals potentially present in a research laboratory poses increased risks from accidental hazardous chemical reactions or explosions. Containers of flammable liquids shall not be drawn from or filled within buildings unless provision is made to prevent the accumulation of flammable vapors in hazardous concentrations. Use refrigerators that have electrical contacts (door switch. 2.4. Flammable Liquids: Fires in solvents and other flammables liquids require dry chemical. Laboratory Operations & Safety Protocol 38 of 65 V1:2010 . 6. These are identified accordingly: 1. energized electrical equipment and other electrical sources require a dry chemical. “Gas” extinguishers. more recently. formation of substances of greater toxicity. Fire Extinguishers. or when a drum is filled. 6. pressurization in closed vessels. both the drum and other equipment must be individually electrically grounded and bonded to each other. Keep flammable liquids away from heat and direct sunlight. formation of toxic vapors. Storage Rules 1. and violent polymerization. 4. C. rubber. thermostat) removed or exteriorized. explosion. 5. B. Most chemical laboratory fire hazards require dry chemical multipurpose extinguishers (ABC) which must be installed in hallways. Flammable liquids must not block lab aisles or exits. or handled in any other way. when they are inadvertently mixed as chemical waste. Hazardous reactions may cause any one or more of the following: heat generation. fire. allowed to stand in open containers. Flammable liquids must not be stored in domestic type refrigerators.
It is prudent to check for incompatibility wherever a change is made in chemical procedures. Incompatibility of chemicals is the prime reason for not storing chemicals on the shelf alphabetically.It is easy to fall into the trap of becoming complacent with certain chemicals used everyday with certain procedures. If there is an accident. the disaster is compounded by the adverse reaction. Laboratory Operations & Safety Protocol 39 of 65 V1:2010 .
Halogenated Solvents. 7. Aqueous solutions with a pH less than or equal to 2. 4. diluted to non-flammable concentrations. Examples of halogenated solvents include methylene chloride. 2. detoxify. Acids and bases containing heavy metals must not be disposed to the sewer system. teratogenic.35 mm per year at 55 oC. capable of detonation or explosive reaction. Reactivity. forms potentially explosive mixtures with water of pH conditions between 2 and 12. absorption of moisture. there must be careful consideration about which wastes can be combined. and carbon tetrachloride. Non-Halogenated Flammable Solvents. or otherwise render the waste nonhazardous. which is less soluble. 7. sodium metal. Different waste materials should be segregated whenever possible. toxic. Corrosivity. inhalation LC50 of 2 mg/L (rats). or spontaneous chemical changes at standard temperature and pressure. before final disposal. liquids which corrode steel at a rate greater than 6.g.Chapter 7 Waste Disposal 7. mutagenic. neutralize. e. solids capable of burning vigorously and persistently after ignition through friction. Recyclable materials should be kept separate from other waste. the more economical is the disposal method for that waste. Ignitability. A waste stream generated from a laboratory procedure should not be combined with other chemical wastes. oxidizers. It is encouraged that laboratories consider ways of reducing the volume of waste or preserving the reuse of the materials through the redesign of experiments. carcinogenic. 3.5. Toxicity. but followed by flushing with copious volumes of water. Laboratory Operations & Safety Protocol 40 of 65 V1:2010 . or dermal LD50 of 200 mg/kg (rabbit). If this is not practical. Halogenated solvents must not be combined with flammable nonhalogenated solvents.5. Acids and Bases. Liquids with a flashpoint less than 60 oC. less than oral LD50 of 50 mg/kg (rats). Disposal of solvents to the sanitary sewer is limited to low-toxicity solvents. The fewer the number of chemicals associated with a waste. Efforts should be made to decontaminate. or suspected carcinogenic. Fatal to humans in low doses. or greater than or equal to 12.3 Handling Specific Wastes Individual Waste Streams. Non-halogenated flammable solvents may be sent to the incinerator and must be free of heavy metals and reactive materials. These should be converted to sulfide. Normally unstable and undergoes violent changes.2 Waste Reduction Minimizing chemical waste generation also minimizes safety hazards. Heavy Metals. reacts violently with water. chloroform. Oils. Only trace quantities of oils associated with cleaning and washing operations should be released to the sanitary sewer. Small volumes of acids and bases can be diluted and discharged in the sanitary sewer.1 Waste Characteristics Hazardous wastes are classified according the following: 1. miscible in water.
to caution the handler.6 Disposal to Sewerage System Avoid discharges to the sanitary sewer of the following: 1. razor blades. Special Handling Considerations. or flammable gas generation should be explained. Do not use abbreviations. pasteur pipettes. Materials that may create a fire or explosive hazard. 7. capillary tubes and scalpels should be placed in puncture-resistant waste collection containers prior to disposal. 4. Liquid waste is to be given in gallons or liters. Sharps. Provide the following information: • Name • Chemical waste classification • Chemical wastes to be kept in the bottle • Name of mentor 3.Sharps. Substances or conditions which could result in an explosion. and solid waste in pounds or kilograms. Filled-up waste bottles should be submitted to the stockroom for signing of clearance at the end of the school year Safety Carriers. Each compound in the waste containers must be listed by its complete chemical name. Carcinogenicity. Obtain waste bottles for chemical wastes from the stockroom 2. Glass collection containers must preferably be stored in rubber safety carriers to protect against breakage and spillage. Waste Characteristics. 3/4 full. A bottle with a barcoded label will be issued by the stockroom personnel to the student. and the approximate percentage of each compound. or teratogenicity should be specified beside “Toxic”. the approximate pH of the waste should be indicated beside “Corrosive” on the label. The amount of waste. including but not limited to syringes.4 Labeling Each container must be labeled with the following information: 1. fire. etc. be sure that the container material is compatible with the waste. 3. 7. If the waste is an acid or a base. All hazardous characteristics of waste should be identified. 2. Laboratory Operations & Safety Protocol 41 of 65 V1:2010 . Amount. heat generation. Principal Constituents. 7. Any toxic or reactive hazards. Corrosive materials with pH less than 5. should be noted. 4. Procedure for obtaining chemical waste bottles 1.5 Waste Containers Containers. If metal or plastic containers are used. The terms most appropriately describing the waste should be included. mutegenicity. giving the container size and indicating whether it is full. Gallon-sized four-liter glass reagent bottles are generally the most convenient. 2.
7 Disposal of Chemical Wastes Non-hazardous Chemical Wastes 1. K. Ti. Solid or viscous materials in amounts to obstruct flow or interfere with operations. Sr. or contamination of sludge of effluent from the wastewater treatment. Si. Al. However. Sr. carbohydrates. Mn. nucleic acids. Mg. Mg. Non-hazardous wastes may be disposed of as ordinary trash. soluble fats and their precursors Inorganic Chemicals • Sulfates: Na. Ca.” 2. NH4 • Borates: Na. Heat discharges which will inhibit biological activities or increase the waste water treatment effluent about 40 oC. NH4 • Carbonates: Na. K. In general. K. Ca Laboratory Operations & Safety Protocol 42 of 65 V1:2010 . the following nonhazardous materials may be placed in ordinary trash bins for disposal: Agarose Alumina Aluminum oxide Ammonium phosphate Calcium carbonate Calcium oxide Calcium phosphate Calcium sulfate Citric acid Dextrin Glycine Magnesium carbonate Magnesium chloride Magnesium sulfate Potassium carbonate Potassium chloride Potassium sulfate Sephadex Silica gel Sodium chloride Sodium citrate Sodium phosphate Sodium sulfate Stannous oxide Starch Sugars Titanium oxide Materials listed below in quantities up to about 100g or 100 mL at a time are suitable for disposal down the drain while flushing with excess water Organic Chemicals • Alkanols with fewer than 4 carbons • Aliphatic aldehydes with fewer than 5 carbons • Amides with fewer than 5 carbons • Aliphatic amines with fewer than 7 carbons • Carboxylic acids with fewer than 6 carbons • Esters with fewer than 5 carbons • Proteins. Fe. Discharges of any toxic material in volume of strength to cause interference with waste treatment processes. Na. 7. Ca. NH4 • Oxides: Mg. the containers should be labeled and marked as “Non-hazardous. Mg. 4. Cu • Chlorides: Ca. Ca.3. K. Mg. Co. Sr. 5. Non-hazardous wastes in liquid form may be disposed of down the drain.
Laboratory Operations & Safety Protocol 43 of 65 V1:2010 .Hazardous chemical wastes Hazardous chemical wastes should be placed and segregated into leak-proof barcoded waste bottles following the classification of the DENR-EMB (See Table 5 in the Appendix). These wastes will be hauled by DENR-accredited transporters for treatment and disposal.
Only organisms under BSL 1 are allowed in the laboratory. Do not wash or reuse disposable gloves. Lab apron must be worn at all times. The secondary container should be sealable and non breakable. 8. 6. 7. Princeton University. 2. only mechanical pipetting devices are permitted. 3. Always wear mask and put on gloves in handling samples. Do not wear contaminated gloves outside the lab. Food is stored outside the work area in cabinets or refrigerators designated specifically for that purpose. a. Before using any of these samples. For bacteria specimens. There are designated refrigerators (Biochemistry and Room 103 NCIC labs) for short-term storage (2 months or less) and a cryogenic container (Rm 103 NCIC lab) for long-term stock storage. Always wash hands after removing gloves and before leaving the lab.Fire extinguisher/blanket Laboratory Operations & Safety Protocol 44 of 65 V1:2010 . The lab apron or coat must be laundered every two weeks. From time to time. 5. 8. the "primary" container should be carried within an outer "secondary" container. know the biosafety level (BSL) of the organism first. Familiarize yourself with the location and operation of the following emergency items for each laboratory. University of Wyoming. University of Tasmania . (Refer to Table 6 of the Appendix) 3. and yeast.3 Transport When transporting samples to or from the laboratory. 8. fungi. Wear appropriate face protection (goggles. 4. mask.Chapter 8 Biological Samples: Handling and Waste Disposal 8. Store the samples in these designated places only to avoid spreading and contamination of bio-free chemicals and solutions.Launceston Campus 1. 2. When leaving the lab. Persons wearing contact lenses should also wear eye protection. face shield or other splatter guard) for anticipated splashes or sprays of infectious materials to the face when agents must be handled outside the BSC (biosafety cabinet).1 General Information Most of the procedures are adapted from Environmental Health and Safety. spray a solution of 70% alcohol (usually ethanol) on the gloves to disinfect.4 Handling 1. there are four levels as specified by US Department of Health and Human Services and National Institutes of Health. 2. Microorganisms refer to organisms of microscopic or sub-microscopic sizes such as bacteria. Remove and change gloves when overtly contaminated or when torn or punctured.2 Storage 1. Do not pipette by mouth. remove and leave lab apron or coat in the lab. and Microbiology Safety and Staff Induction Manual. First aid kit b. Samples must be properly labeled to immediately identify the organism present. virus.
A heat sensitive indicator (autoclave tape) should be used in every load. The standard autoclave cycle used in this laboratory is 15 psi for 15 minutes. Wear appropriate personal protective equipment.15 minutes prior to use. if the cabinet is not left running. 9. 7. Adjust the working height of the stool so that the worker's face is above the front opening. 11. Exits 8. A laminar flow cabinet is also available in the Biochemistry lab. 2. 4. Disinfect work surface with 70% alcohol or other suitable disinfectant. and gloves. The working area should be wiped with disinfectant at the beginning and end of the laboratory session. The BSC in the laboratory is a Class 2 cabinet designed to provide personnel. 2. Before using the equipment. Wipe the bottom and side of the hood surfaces with disinfectant when work is completed. It is housed in the isolation room in C305. To prevent accidents. 3. Always wear safety glasses when opening the pressure cooker. 3. Work at a moderate pace to prevent the air flow disruption that occurs with rapid movements. Laboratory Operations & Safety Protocol 45 of 65 V1:2010 . Consider the materials necessary for the planned work in the cabinet. put on gloves. or brought into the laboratory without permission of the instructor or laboratory in-charge. wait until the pressure drops to ~5 psi before opening the exhaust valve. 11. The lid should only be open when the pressure is zero. indicate the date. Delay manipulation of materials for approximately one minute after placing the hands/arms inside the cabinet. Gas isolation switch d. mask. 10. splashes and the production of aerosols. working with materials from the clean to the dirty side. time and contents of the cycle. Laminar Flow Cabinet/Biosafety Cabinet (BSC) 1. Princeton University) 1. 8. Working in a BSC (adapted from Environmental Health and Safety. 9. 6. Place items into the cabinet so that they can be worked with efficiently without unnecessary disruption of the air flow. Turn the cabinet on for at least 10 . call the instructor immediately. 4.Eyewash station e. 12. make sure that the door of the isolation room is closed to minimize the disruption of the airflow.c. One pressure cooker is designated for sterilization purposes while another is solely used for decontamination. Always use heat safety gloves when removing items from the pressure cooker. Do not disturb the airflow by covering any of the grillwork with materials. This applies particularly to the flaming of the bacteriological loop: the loop should be drawn gradually from the cooler to the hotter parts of the flame. environmental. and sample/product protection. 8. Minimize the frequency of moving hands in and out of the cabinet. For minor spills. At a minimum. Leave for 10 minutes and then mop up. cover spill with paper towel and pour on disinfectant. Carry out procedures so as to minimize the risks of spills. this will include a laboratory apron/coat. 5. 10. In the logbooks of either pressure cooker. 6. Always wash your hands before leaving the laboratory. If you have an accident of any kind.5 Laboratory Equipment Autoclave 1. 2. No slides or cultures are to be taken from. 5.
A container for used items (pipet tips. 8. In the event of spill incidents. or remove used needles from disposable syringes or otherwise manipulate such units by hand before disposal. liquid wastes are thrown down the drain while solid wastes are thrown in trash bins. Clean the working area using surface decontamination procedure. shear. The standard decontamination cycle used in this laboratory is 15 psi for 15 minutes. and other materials used in the microbial activity must be decontaminated. tongs. or forceps – not directly with hands. After decontamination. 3. Princeton University) Assessing a Biohazard Spill The biological nature of the spill. if possible. (adapted from Environmental Health and Safety. and water or as thorough as sterilization. 4. bleach. pipet. For example. 3. culture loops. within the day or the day after. recap. Place full containers in an autoclave bag and sterilize before disposal in waste boxes. A decontamination procedure can be as simple as clean-up with a solution of detergent. Do not bend. and is infection likely to be acquired by the respiratory route? The physical nature of the spill. The materials must be decontaminated immediately. preferably made of glass or other autoclave-compatible materials. After the microbial laboratory activity. put the glassware and other materials directly touched by culture media or contaminated with microorganism in a box or leave them inside the BSC.7 Clean-up Procedure 1. how pathogenic are the organisms contained or likely to be contained in the spill. For example. Liquid Decontamination • • • Add liquid chlorine bleach to provide a final 1:10 dilution Let stand at least 20 minutes Discard down the drain Surface Decontamination • • Wipe with 1:10 dilution of chlorine bleach. has the spill resulted from a container knocked over on a bench with low potential for the generation of aerosols. 2. 2. consummable items. Sterilization is done using the pressure cooker designated for decontamination purposes. The container must be covered by aluminum foil. or smashed in some way with a greater potential for the generation of aerosols? Laboratory Operations & Safety Protocol 46 of 65 V1:2010 .6 Waste Disposal 1. break. Dispose of needles and syringes in the puncture resistant container provided in the laboratory for this purpose.8. others) must be prepared. 2. Handle broken glassware with brush and dustpan. or has a container been dropped. the following steps must be considered. or Wipe with 70% alcohol 1. All glassware.
1. 4. 6. If hands have been contaminated. pneumoniae that has been dropped onto the floor would be considered a "major biohazard spill". 5. Remove any contaminated clothing and wash any contaminated body surfaces. Dispose the absorbent material by sealing them in a plastic bag and placing it in a trash bin. 1. Wipe over general area again with paper towel dampened with disinfectant. Lay paper towels wetted with disinfectant onto the spill and leave for 30 minutes. Major biohazard spills Generally considered to be spills of major risk with larger volume and considerable production of splashes and aerosols. 1. 6. 4. vaginal secretions. 10 ml broth culture of E. Laboratory Operations & Safety Protocol 47 of 65 V1:2010 . Clean and disinfect the area with freshly prepared 1:10 (bleach: hot water) solution. warn others of spill and all must leave the room immediately. Don’t forget to wear gloves. semen. Minor biohazard spills Generally considered as a spill of minimally hazardous material with low potential for generation of aerosols. In the event of spillage. Use disinfectant to wipe over areas around the spill that are likely to have been contaminated with splashes and aerosols. mop up spill and place contaminated materials into autoclave bag. Put on gloves. 3. vomit. Do not re-enter the room until a minimum of 30 minutes has elapsed. 3. Remove gloves and wash hands. 4. For example. 5. Wash the hands thoroughly with hot water and soap. 7. Close doors and place a "DO NOT ENTER" sign on the door. face masks and safety glasses" before entering the spill area. Discontinue working in the immediate area. Do not pour disinfectant directly onto the spill as this may create additional aerosols. coli that has been knocked over on the bench would be considered a "minor biohazard spill" whereas a 500 ml broth containing S. 2. 8. 8. Hold breath. Remove and replace any contaminated protective clothing. Always wear gloves in dealing with body fluids. Treat all body fluids as if they are infectious. 9. 5. After 10 minutes.The volume of liquid spilt. 8. 2.8 Body Fluids Body fluids include blood. and oral or nasal secretions. first wash hands with strong detergent followed by generous spray of 70% alcohol. gloves. urine. Dispose the gloves by sealing them in a plastic bag and placing it in a trash bin. 3. Notify laboratory in-charge(preferably) or other faculty member The clean up team should all don "gowns. Lay down absorbent material wetted with disinfectant over the spill and allow to sit for 10 minutes. Determine the extent of the spill: pour disinfectant around the edge of the spill and allow it to run "into the spill". feces. put an absorbent material over the fluid. 2. 7.
overnight stays are discouraged. fill-in. A.3 Instrument Authorization Form Students who wish to use an instrument in the department are required to be checked-in first by the faculty-in-charge. work that will go beyond 12 midnight is considered overnight. As a rule.1 in following pages serves as the Overtime/Overnight Request form. This will allow the office to inform the University administration and security. If your supervisor allows it. The FORM A. and submit to the instrument manager of the Chemistry Department. overnight stay shall be considered. It is the supervisor’s (faculty’s) responsibility to ensure that the student receives prompt treatment of the injury by obtaining first aid or assistance to medical treatment. Photocopy duplicates (for the Department and for yourself).2 in following pages serves as the Accident Report form. If the injured person is not able to do so. as long as there are still faculty in the building to let you out of the building. A.m. fill-in. These require you to fill in the form below and seek final approval from the Department Chair. hospitalization. the Physical Plant. At least two people should stay for overnight work. Work that will go beyond 10 p.m.1 Form for Overtime or Overnight Lab Work Schmitt Hall is opened to the public by the building manager by 6:00 a. is considered overtime.. Any injury resulting in lost work time. Photocopy duplicates (for the Department and for yourself).2 Form for Reporting Lab Accidents All personal injuries and accidents should be reported in order that corrective action may be taken to minimize the probability of recurrences. and locked by the security guard at 10:00 p. a co-worker should notify the supervisor as soon as possible. Laboratory Operations & Safety Protocol 48 of 65 V1:2010 . Overtime forms may be processed within the day of the scheduled overtime.m. and submit to the secretary of the Chemistry Department. It is also the supervisor’s responsibility to file an injury/accident report with the Chemistry Department. The FORM A.m. Try to work out a schedule that will go from 6:00 a. or medical treatment in excess of first aid will be investigated by the Chemistry Department to determine liability or the possible need for corrective action. File injury/accident reports with the secretary of the Chemistry Department.m. to 10:00 p. and submit to the secretary of the Chemistry Department. It is the student’s/researcher’s responsibility to notify his supervisor immediately of any work-related injury or illness. while overnight forms must be handed in to the Department secretary at least 2 days before the event.3 serves as the Instrument Authorization form.m.Appendices A. If an experiment requires longer than 15 hours. fill-in. Photocopy triplicates (for the Department. and for yourself). you may work until 10 p. The FORM A.
1 : Overtime/Overnight Request Full Name (please print) Date of Request Nature of Request (encircle one) Overtime Overnight Time of Event Date of Event Name of Accompanying Person(s) Signature(s) List of Special Equipment. After filling-in the above items. get the following signatures: (print name and sign) Your Signature Your Supervisor Building Manager Chair Note: Trplicate! Laboratory Operations & Safety Protocol 49 of 65 V1:2010 .FORM A. Chemicals. and Methods Reason: State why this work cannot be done during regular building hours.
FORM A.2 : Accident Report
Full Name (please print) Date of Report
Lab Class and Section
Time of Accident
Date of Accident
Name of Injured Person(s)
List of Equipment & Chemicals Involved
Narrate the accident chronologically and in detail. Supply all the pertinent facts.
What first aid or medical attention were applied?
After filling-in the above items, get the following signatures: (print name and sign) Your Signature Witness 1 Witness 2 Chair
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FORM A.3 : Instrument Authorization Form
Ateneo de Manila University School of Science and Engineering Department of Chemistry
INSTRUMENT AUTHORIZATION FORM Student:____________________________________ Instrument:_____________________ Name of Mentor:_____________________________ Mentor’s Approval:______________ Purpose of Instrument Use: ___________________________________________________ __________________________________________________________________________ Waiver: I have read and I fully understand the “Policies on the Use of the Department’s Facilities and Equipment.” I _______ agree to follow the guidelines and procedures _______ do not agree to follow the guidelines and procedures because:______________
INSTRUMENT CHECK-IN Date of Check in: ________________________ Faculty in-charge: ________________________ ____Instrument General Working Principles ____Instrument Parts ____ Safety and Precautionary Measures ____ Short Notes of the Procedure ____ Important Reminders and Considerations Remarks:____________________________________________________________________---------------------------------------------------------------------------------------------------------------AUTHORIZATION CERTIFICATE Date: __________________________________ Faculty in-charge: ___________________________ I hereby certify that Mr./Ms. _____________________________ is authorized to use the ____________________________________. Limitations of use:_______________________________________________________________ -------------------------------------------------------------------------------------------------------------------------INSTRUMENT CHECK-OUT Date of Check out:____________________ Faculty in-charge: _________________________ ____Instrument Parts ____Instrument Accessories ____Consumables ____Working environment
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Figure 1: Lab benches for General Chemistry Lab. Each bench has its own locker, equipment set, hood, sink, power and gas lines, and broom for sweeping up broken glass.
2: Fume hoods for (A) the Analytical Lab, and (B) General Chemistry Lab. The fume hoods are located either on the sides of the laboratory rooms (A), or individually, for each lab bench (B).
Figure 3: Power, gas,
and water lines. These are color coded as follows: RED for power lines, GREEN/BLUE for water lines, and YELLOW for gas lines. The valves are also similarly color coded.
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Figure 4: Fire extinguishers (A) and showers (B) are placed prominently in all labs. B Figure 6: Aspirator set-up for the clean-up of mercury spills. Laboratory Operations & Safety Protocol 53 of 65 V1:2010 . as shown here in the Organic Lab (A) and General Chemistry Lab (B). A Figure 5: with exits also in all Floor plans directions to fire (red arrows) are placed prominently rooms.
Figure 7: The new (March 2004) fire alarm system in Schmitt Hall: Breaking the cover and pushing the fire alarm button triggers the alarm. There are several throughout the building. Occupants are requested to be familiar with the location of the push button nearest their office/lab. Figure 8: GHS Pictograms and Hazard Classes Laboratory Operations & Safety Protocol 54 of 65 V1:2010 .
Figure 9: Compressed gas tanks tethered properly and capped. Figure 10: Logical computer hardware setup Laboratory Operations & Safety Protocol 55 of 65 V1:2010 . Note the dolly on the right for proper transportation of gas cylinders.
Figure 11: Overview of CMIS Requesting Procedure Laboratory Operations & Safety Protocol 56 of 65 V1:2010 .
RAs. Environmental Management Bureau (EMB) Chemical waste management of toxic and hazardous wastes (See Appendix). ban on incineration Laboratory Operations & Safety Protocol 57 of 65 V1:2010 . formulation and application of standards for the transport and disposal of effluent. EMB Implementing Agency Philippine National Police Coverage Restriction on purchase of nitrates. Regulation on the use. CMIS User-levels CMIS Feature Access to chemical information Access to storage locations Access to stock levels Setting up and modification of data Requesting services Chemical request management Password management Reporting services Administrative functions Access to Hazardous Waste Inventory System (HWIS) 1 x User-level 2 3 x x x x x x x x x x x x 4 x x x x x x x x x x x 1-Guest: for undergrad and grad students. List of laws. technician-in-charge of CMIS Table 2. storage and disposal of toxic chemicals and hazardous and nuclear wastes Air emissions. etc. lab managers. 2-TAs. junior faculty 3-Department secretary. development of guidelines for re-use of wastewater etc. rules and regulations which govern various aspects related to chemicals. Abatement and control of pollution water bodies. Law. Rule or Regulation RA 8294: Regulation on explosives and materials used in the manufacture of explosives (1997) RA 6969: Toxic Substances and Hazardous and Nuclear Wastes Control Act (1990) DAO 92-29: Implementing Rules and Regulations of RA 6969 RA 8749: Philippine Clean Air Act (1999) DAO 00-81: Implementing Rules and Regulations of RA 8749 RA 9275: Clean Water Act (2004) DAO 05-10: Implementing Rules and Regulations of RA 9275 EMB Water quality management in all water bodies. movement. nitric acid. sewage and septage offsite.Tables Table 1. senior faculty 4-Administrators. technicians.
Lysergic acid 8. Rule or Regulation DAO 97-39: Chemical Control Order for Cyanide and its Compounds Implementing Agency EMB Coverage Importation. and other waste depots. manufacture. transport. possession or use of prohibited and regulated drugs and precursor chemicals RA 6425: Dangerous Drugs Act (1972) Philippine Drug Enforcement Agency (PDEA). Treatment. Isosafrole 7. PCB articles and PCB packaging in commercial buildings and facilities. Treatment. This also includes the use and possession by electric utilities and suppliers and the generation. Implementing Rules and Regulations for specific substances Law. non-PCB equipment. List of PDEA-controlled chemicals 1* 1. Piperonal 12. use and distribution of cyanide and cyanide compounds. collection. manufacture of asbestos. sale. transport. distribution and use of PCBs. Importation. PCB-contaminated equipment. DAO 00-02: Chemical Control Order for Asbestos EMB DAO 04-01: Chemical Control Order for Polychlorinated Biphenyls (PCBs) EMB Table 4. transportation. Sulfuric acid 9. delivery. sanitary landfills. Ergotamine 6. Potassium Permanganate 2* 1. Regulation of the importation.4-Methylenedioxyphenyl-2-propanone 9. N-Acetylanthranilic acid 3. Toluene Laboratory Operations & Safety Protocol 58 of 65 V1:2010 . Norephedrine 10. storage. Hydrochloric acid 5. Piperidine 8. This includes proper segregation. Phenylacetic acid 7. 1-Phenyl-2-propanone 11. distribution. PCB equipment. storage and disposal of asbestos-containing materials or asbestos containing wastes. sale. storage and disposal of cyanide-bearing or cyanide contaminated wastes. Acetone 2. formulation of guidelines and targets for solid waste avoidance and volume reduction and setting up of controlled dumpsites. Anthranilic acid 3. storage. administration. 3. manufacture. Methyl ethyl ketone 6.RA 9003: Ecological Solid Waste Management Act (2000) DAO 01-34: Implementing Rules and Regulations of RA 9003 EMB Management of solid wastes. Ephedrine 4. transfer. Ergometrine 5. Acetic anhydride 2. Importation. treatment and disposal of solid waste. Department of Health (DOH) Table 3. Ethyl ether 4. treatment and disposal of PCB wastes.
Wastes with inorganic chemicals Selenium and its compounds Arsenic and its compounds Barium and its compounds Cadmium and its compounds Chromium compounds Lead compounds Sulfuric acid with pH = 2.5 Ammonium hydroxide with pH = 12.0 mg/L based on analysis of an extract B201 B202 B203 B204 B205 B206 B207 B208 B299 C301 C302 C303 C304 C305 C399 D401 D402 D403 D404 D405 D406 Laboratory Operations & Safety Protocol 59 of 65 V1:2010 .5 Lime slurries with pH = 12. Pseudophedrine 14.0 Mixture of sulfuric and hydrochloric acid acid with pH = 2. Alkali wastes Caustic soda Potash Alkaline cleaners Ammonium hydroxide Lime Slurries Other alkali wastes D. Safrole *The salts of the substances in the list whenever the existence of such salts is possible (The salts of hydrochloric acid and sulfuric acid are specifically excluded).0 Caustic soda with pH = 12.0 Acid wastes other than B201 to B208 with pH= 2.0 Other inorganic acid with pH = 2.0 Hydrochloric acid with pH = 2.0 mg/L based on analysis of an extract Includes all wastes with a total Pb concentration > 5.0 mg/L based on analysis of an extract Includes all wastes with a total Cr concentration > 5.0 mg/L based on analysis of an extract Includes all wastes with a total Ba concentration > 100.0 Phosphoric acid with pH = 2. Table 5.5 Alkali wastes other than C301 to C306 with pH = 12.5 Potash with pH = 12.13.0 mg/L based on analysis of an extract Includes all wastes with a total As concentration > 5.5 Includes all wastes with a total Se concentration > 1.0 mg/L based on analysis of an extract Includes all wastes with a total Cd concentration > 5.0 Hydrofluoric acid with pH = 2. DENR Classification of Hazardous Wastes Class A: Wastes with cyanide Wastes with cyanide Description Waste Number A101 Waste containing cyanide with a concentration >200 ppm in liquid waste B: Acid wastes Sulfuric acid Hydrochloric acid Nitric acid Phosphoric Acid Hydrofluoric acid Mixture of sulfuric and hydrochloric acid Other inorganic acid Organic acid Other acid wastes C.0 Organic acid with pH= 2.5 Alkaline cleaners with pH = 12.0 Nitirc acid with pH = 2.
It is normally unstable and readily undergoes violent change without detonating. it generates toxic gases.continuation (Table 5) Mercury and mercury compounds Other wastes with inorganic chemicals Includes all wastes with a total Se concentration > 0. It forms potentially explosive mixtures with water. It reacts violently with water. which when exposed to pH conditions between 2 and 12. When mized with eater. Reactive chemical wastes Oxidizing agents E501 Reducing agents E502 Laboratory Operations & Safety Protocol 60 of 65 V1:2010 . It is a cyanide (CN) or sulfide (S) bearing wastes. vapors and fumes in a quantity that poses a danger to human health D407 D499 E. zinc compounds. beryllium and its compounds. 4. vapor or fumes in a quantity sufficient to present a danger to human health. It reacts violently with water. it generates toxic gases. copper compounds.5 can generate toxic gases. metal carbonyls. thallium and its compounds. 4.5 can generate toxic gases. 3. 3. 2.2 mg/L based on analysis of an extract. tellurium and its compounds. which when exposed to pH conditions between 2 and 12. vapors and fumes in a quantity that poses a danger to human health Includes all wastes that are known to contain oxidizing or reducing agents in concentration that cause the waste to exhibit any of the following properties: 1. It forms potentially explosive mixtures with water. inorganic fluorine compounds excluding calcium fluoride Includes all wastes that are known to contain oxidizing or reducing agents in concentration that cause the waste to exhibit any of the following properties: 1. vapor or fumes in a quantity sufficient to present a danger to human health. It is normally unstable and readily undergoes violent change without detonating. Refer to CCO Wastes containing the following chemicals: antimony and its compounds. 2. When mized with eater. It is a cyanide (CN) or sulfide (S) bearing wastes.
1. Trichloroethane. non-halogenated solvents: Xylene. 1. soaps. and stabilizers containing Chromium and Lead. toluene.1. tricholorethylene. or 2) capable of detonation or explosive decomposition at a temperature of 20 o Celsius and Pressure of 1 atm. Carbon disulfide. it also includes all still bottoms from recovery of these solvents and solvent mixtures. methylene chloride. Highly reactive chemicals Includes all other wastes that exhibit any of the properties described for D501. ethyl benzene. methanol. ethyl ether.hexanol.1. it also includes all still bottoms from recovery of these solvents and solvent mixtures. methyl isobutyl ketone. chlorinated flouro-carbons if they contain a total of 10% or more (by volume) of one or more of the above before use. n-butyl alcohol. Laboratory Operations & Safety Protocol 61 of 65 E503 E599 F601 F602 F603 F610 F699 G703 G704 V1:2010 . chlorobenzene. cresylic acid. Other mixed Includes all aqueous-based wastes that also meet one or more of the subcategories. 2-ethoxy ethanol and 2 nitropropane and other non-halogenated organic solvents if they contain a total of 10% or more (by volume) of one or more of these solvents before use. carbon tetrachloride. Waste organic solvent Halogenated organic solvents Includes the ff. acetone. spent halogenated solvents: Tetrachloroethylene.2 Trichloroethane. ethyl acetate. G. caustic washings and sludge or wastewater and sludge from cleaning of tubs and equipment used in the formulation of ink from pigments. cresole. benzene. D502. pyridine. F:Inks/Dyes/Pigments/Paint/Latex/Adhesives/Organic sludge Aqueous based Includes all aqueous based wastes that also meet one or more of the subcategories Solvent based Includes all solvent based wastes that also meet one or more of the subcategories Inorganic pigments Includes all wastewater treatment sludge from the production of inorganic pigments Ink formulation Includes all solvent washings and sludge. and D503. Non-halogenated organic solvents Includes the ff.continuation (Table 5) Explosive and unstable chemicals Includes all wastes that are 1) capable of detonation or explosive reaction when subject to a strong initiating source or when heated under confinement.isobutanol. nitrobenzene.2. driers. cyclo.
all waste from facilities that process an average of 2500 fowls or more.continuation (Table 5) H. and L. D. E. Wastes whose hazardous substances are physically immobilized by consolidation to reduce the surface area of the wastes in order to meet the waste acceptance criteria Wastes whose hazardous substances are chemically immobilized through chemical bonds to an immobile matrix or chemical conversion to meet the waste acceptance criteria Wastes whose hazardous substances are physically immobilized by enveloping the waste in a non-porous. sub-categories M504 and M505. all waste from poultry farms with an average of 5. Includes all establishments that generate an average of 50 kg per day Includes all wastes from establishments that generate. transport or treat more than 200 L of waste oil per day except vegetable oil and waste tallow H801 H802 I101 J: Containers Containers previously containing toxic chemical substances Waste containers that used to hold the toxic chemical substances listed in Classes A. impermeable material in order to store hazardous wastes until such time that a proper disposal facility is available. Containers that used to contain Polychlorinated biphenyl (PCB) are categorized as L406 and excluded from this sub-category. Oil Waste oils Includes all wastes from animal feed lots containing an average of 100 or more animals. and the chemicals listed in the Priority Chemical List. All wastes from commercial slaughter houses that slaughter an average of 500 or more animals per year .000 fowls or more. J201 K: Immobilized Wastes Solidified wastes and polymerized wastes K301 Chemically fixed wastes K302 Encapsulated wastes K303 Laboratory Operations & Safety Protocol 62 of 65 V1:2010 . Putrescible/Organic Wastes Animal/abattoir waste Grease trap wastes from industrial or commercial premises I.
heptachlor. mirex. Expired pharmaceuticals and drugs stocked at producers and retailers’ facilities. toxaphene. bloods and body fluids).1. L401 Ozone depleting substances L402 PCB wastes L406 M: Miscellaneous Wastes Pathogenic or infectious wastes Includes pathological wastes (tissues. endrin. Refer to CCO. Waste blue and brown asbestos fibers. Includes all wastewater sludge from production of pesticides other than those listed in M505.continuation (Table 5) L: Organic chemicals Wastes with specific nonhalogenated toxic organic chemicals Non-liquid waste containing the following: .2-diphenylhydrazine benzene Waste chlorofluoro carbons (CFCs) and halons.organs. Refer to CCO. hexachlorobenzene. Recovered coolant containing chlorofluoro carbons (CFCs) or halons Wastes contaminated with PCB and waste products containing PCB. chlordane. Waste pesticides listed in the Stockholm Convention (POPs Convention) such as aldrin. dieldrin. infectious wastes and sharps Wastes containing friable asbestos. Waste pesticides other than M505. and DDT. fetuses.Tri-butylin . M501 Friable asbestos wastes M502 Pharmaceuticals and drugs M503 Pesticides M504 POPs (Persistent Organic Pollutants) pesticides M505 Laboratory Operations & Safety Protocol 63 of 65 V1:2010 .
Table 6. Summary of Recommended Biosafety Levels for Infectious Agents1 Laboratory Operations & Safety Protocol 64 of 65 V1:2010 .
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