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Chemistry Department Loyola School of Science & Engineering Ateneo de Manila University Version 2010
Preface to the 2010 Edition
In 2004, the government started to implement various laws, rules and regulations covering a broad range of activities related to the purchase, use, storage, waste management, and disposal of chemicals. The increased regulation has led the University to implement a common policy and procedure to address these various concerns. The Chemical Management and Inventory System (CMIS) was developed to address the need for accurate inventory, efficient purchasing, management, and compliance with various laws as well as the management of hazardous chemical wastes. The changes in the legal and regulatory climate have also contributed to the changing culture of safety which now puts emphasis on experiment planning, risk assessment, hazard consideration and chemical waste minimization. In this edition, information on different sections (i.e. Emergency Protocols, Use of Instruments and Waste Disposal) was updated. A section on the CMIS and Handling of Biological Samples was added. I wish to thank Ms. Mailyn Terrado for the section on Dealing with Biological Samples and Wastes. I also wish to thank Dr. Regina C. So and Dr. Fabian M. Dayrit for taking the time to proofread the manual. Jaclyn Elizabeth Santos 18 June 2010
Preface to the 2002 Edition
his manual is intended to be a safety reference document for lab workers at the Chemistry Department of the Ateneo de Manila University. It is mainly adapted from the Laboratory Safety Manual at the University of North Carolina at Chapel Hill, and the memo on Operating Practices issued by Dr. Ma. Assunta Cuyegkeng when she was Chair of the Department in 1996—practices that have served the Department well since the time of Fr. Schmitt. This manual provides basic information about hazards encountered in the lab and safety precautions to prevent accidents. It also contains the description of faculty, student, and staff roles in ensuring a safe working environment in the lab. It is not possible to cover all safety and emergency scenarios, but this manual should, nonetheless, serve as the starting point for the faculty in developing their own safety plans specific to the materials and procedures in their laboratories. It is often a sticky effort to balance convenience and control with regards to safety issues in the lab, but when all is said and done, the basic rules are alertness and vigilance, common sense, conservation of resources, and concern for other workers in the lab.
Francis Ted Limpoco
18 November 2002
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1.1 1.2 1.3 1.4 1.5 Safety Awareness The Role of Faculty The Role of a Safety Officer The Role of Lab Technicians The Role of Students/Researchers
6 6 7 8 9
2. Emergency Protocols
2.1 2.2 2.3 2.4 2.5 2.6 2.7 2.8 Dealing with Emergency Emergency Telephone Numbers Fire Emergency Chemical Spill Escape of Noxious Gases First Aid Medical Attention After First Aid Reporting Accidents and Injuries
10 11 12 13 14 14 15 16
3. The Use of Building & Facilities
3.1 3.2 3.3 3.4 3.5 3.6 Schmitt Hall Room Addresses Use of the Building Access Keys Special Rooms Use of Instruments
17 18 19 19 20 20
4. General Safety Principles
4.1 4.2 4.3 4.4 4.5 4.6 4.7 4.8 4.9 Overnight Operations Working Alone Eating, Drinking, and Smoking Housekeeping Warning Signs and Labels Shielding for Safety Compressed Gases Systems Under Pressure Cold Traps and Cryogenic Hazards
23 23 23 23 23 24 24 24 25 25
5. Chemical Management and Inventory System
5.1 5.2 Chemical Management and Inventory System Controlled Chemicals
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6 Waste Disposal 8.3 Form for Overtime/Overnight Lab Work Form for Reporting Lab Accidents Instrument Authorization Form 48 48 48 48 Figures Figure 1 Figure 2 Figure 3 Figure 4 Figure 5 Figure 6 Figure 7 Figure 8 Figure 9 Figure 10 Figure 11 Lab Benches Fume Hoods Power.7 Handling Explosive/Reactive Materials 30 30 32 33 35 36 37 38 7.2 Waste Reduction 7.3Transport 8.2 A.3 Handling Specific Wastes 7.5 Waste Containers 7.4 Handling 8.6 Disposal to Sewerage System 7. Biological Samples: Handling and Waste Disposal 44 8.2 Routes of Exposure 6. Handling of Chemicals 6.8 Body Fluids 44 44 44 44 45 46 46 47 Appendices A.5 Handling Carcinogens 6. Gas.2 Storage 8.4 Handling Toxic Materials 6.3 Protective Clothing and Equipment 6.6 Handling Flammable Materials 6.1 General Information 8. Waste Disposal 7.7 Clean-up Procedure 8.1 Hazardous Chemicals 6.4 Labeling 7.1 Waste Characteristics 7.7 Disposal of Chemical Wastes 40 40 40 40 41 41 41 42 8.6.5 Laboratory Equipment 8.1 A. and Water Lines Fire Extinguishers & Showers Floor Plans with Fire Exits Aspirator Set-up for the Clean-up of Mercury Spills Fire Alarm Compressed Gases CMIS’ Logical Computer-Hardware Set-up Overview of Requesting Procedure Overview of CMIS Requesting Procedures 52 52 52 52 53 53 53 54 54 55 55 56 Laboratory Operations & Safety Protocol 4 of 65 V1:2010 .
rules and regulations which govern various aspects related to chemicals 57 Table 3 Table 4 Table 5 Table 6 Implementing Rules and Regulation for specific substances List of PDEA-controlled chemicals DENR Classification of Hazardous Wastes 58 58 59 64 Summary of Recommended Biosafety Levels for Infectious Agents References 65 Laboratory Operations & Safety Protocol 5 of 65 V1:2010 .Tables Table 1 CMIS User-levels 57 57 Table 2 List of laws.
Inspect the equipment in the students’ and common lockers. a. Not entering the stockroom. shower.g. Set a good example by being enthusiastic about safety. which frequently results in carelessness. Maintain discipline and enforce the Department’s operational and safety rules. water. c. “We were not told.) 4. 3. As the saying goes: “An ounce of prevention is worth more than a pound of cure”. safety glasses. and power lines. Common sense is still the guiding norm. Ask if students have special medical conditions that may interfere with lab work/safety. You must consider not just yourself.2 The Role of Faculty The faculty is responsible for the administration of the safety program of the Chemistry Department. This includes showing the locations of the fire extinguisher. but also the other people involved— always consider their safety and convenience as well. 1. and evacuation plan. and check-out the same at the end of the term. Brief students on the lab operations and safety protocols. be alert to unsafe conditions and actions and call attention to them so that corrections can be made as soon as possible. This attitude can lead to a false sense of security. Every lab worker has a basic responsibility to himself and his colleagues to plan and execute lab operations in a safe manner. Orient students in the proper use of the lab facilities and instruments. d. Thus. Laboratory Operations & Safety Protocol 6 of 65 V1:2010 .Chapter 1 Introduction T 1.” when it is obvious what you should do in a given situation. Wearing the prescribed safety gear: safety glasses and aprons. b. and by observing all safety rules and wearing personal protective equipment (e. NO SET OF RULES can cover all situations. and the locations of the valves for the gas. Most of the rules/responsibilities stated here are nothing more than common sense and concern for others. Over familiarity with a particular laboratory operation may result in overlooking or underrating its hazards. such as: a. 2. Check-in students at the start of the term. As lab instructor. Remind students to settle the lab breakage deposit immediately. Please do not argue.1 Safety Awareness he most important rule is that everyone involved in lab operations—from the highest administrative level to the individual workers—must be safety minded. (See Figures 1-5. Safety awareness can become part of everyone’s habits only if the issue of safety is discussed repeatedly and only if senior and responsible staff demonstrate a sincere and continuing interest in safety. c. the faculty should be in the laboratory for the entire laboratory period. Not eating or drinking during the lab. closed-toe shoes and apron). eyewash. b. Every instructor should: 1.
the faculty not only guides the student in the direction of his research project. Orient the students on the chemical waste disposal procedures of the department. Accidents resulting in medical treatment or observation should be recorded. 7. Conduct a briefing on safety issues at the start of the class. be alert and vigilant for unsafe conditions. stating the cause. Determine whether researchers need to work continuously after office hours or overnight. fire alarms. and take effective corrective action promptly. 10. 4. regularly check safety facilities and equipment (i. storage and disposal of the Chemistry department. inspect often and intelligently. Manage the CMIS. Notify the parents/guardian of the injured person about the incident. Coordinate with the University Physical Plant and Office of Adminitrative Service regarding the disposal of chemical wastes with a DENR-accredited waste transporter and treater.g. Orient researchers with the instrument techniques and protocols. 7. no eating and drinking in the lab/stockroom) and wearing protective equipment (e. 3. Review and update safety protocol and procedures in the department. The mentor/supervisor should: 1. Train students in proper lab techniques. and by observing all safety rules (e. spill kits) 4. Set a good example by being enthusiastic about safety.5. eyewear. Orient researchers with the general operations and safety procedures at the Chemistry Department. 8. Report all accidents. The mentor is responsible for checking-in researchers on the instrument or ensuring that they are checked-in by a competent/expert user of the instrument. eye wash stations. In case of accidents/emergencies.3 The Role of a Safety Officer The safety officer is responsible for activities related to safety. 2. 6. effect. Discuss with researchers any safety risks involved in the experimental procedures that will be carried out and the materials that will be used in the research. safety showers. 8. waste minimization and where appropriate. Together with the laboratory supervisers and staff. Undertake an annual review of the laboratory experiments which are included in curricular offerings to consider aspects of safety.e. A formal written report to the Chair should be made of all accidents. 5. especially those that may pose safety hazards. 6. implement proper first aid measures and if necessary coordinate proper and speedy conveyance of the injured person to a medical facility. Regularly orient science majors (both undergraduates and graduate students. compliance recommend appropriate improvements. check that the system is working satisfactorily. redesign or replacement. but also ensures that the student is not exposed to safety risks in doing his research. 1. chemical management. where recommended). etc. first aid kits.g. Anticipate and prevent accidents at all times. 3. especially those doing their thesis) research assistants and faculty members regarding chemical safety and chemical waste management and minimization. hoods. 2. 9. The safety officer should: 1. Carefully review all laboratory experiments for possible safety problems before experiments are assigned. and Laboratory Operations & Safety Protocol 7 of 65 V1:2010 . Conduct an inventory of chemical wastes generated by the department. fire extinguishers. and ensure there are logistical provisions for their safety in such cases. and recommendations for the prevention of reoccurrence. As mentor/research supervisor. aprons.
and sand for cleaning up spills. 7. supplies. Prepare a periodic report (annually or as required) regarding the status of the chemical inventory and chemical waste of the department. Regularly check current loads of aircons. Provide technical services at Schmitt Hall. Set a good example by being enthusiastic about safety. glassware repairs. Prepare chemical unknowns which must be carefully coded and recorded. b. and plastic. This includes fire extinguishers. 4. 1. Dispense chemicals. Ensure that teaching laboratories are provided with properly-labeled chemical waste bottles. Report all accidents. Inform the immediate supervisor well in advance of the need to order supplies before they run out. c. Assist the faculty in inspecting the student laboratory lockers/equipment at the beginning and at the end of the semester.4 The Role of Lab Technicians The lab technician and/or stockroom manager should also be present during the entire laboratory period to assist the faculty in managing the experiment and administering the safety procedures of the Department.g.. 8. especially for laboratories. Together with the safety officer. stating the cause. In case of accidents/emergencies. effect. Prepare with accuracy chemicals and solutions for laboratory work. 5. special containers for chemical and broken glass wastes. Together with a trained professional. no eating and drinking in the lab/stockroom) and wearing protective equipment (e. d. According to established norms. inspect all safety implements according to the recommended frequency. Handle the renewal of licenses required by government authorities. fume hood. eye wash. investigate and repair simple electrical circuits and minor electrical breakdown. Make plumbing repairs. Exercise supervision over students with regard to the stockroom transactions and general order in the laboratories. and by observing all safety rules (e.9. eyewear. materials. a. Ensure that the CMIS is updated. A formal written report to the Chair should be made of all accidents. 2. etc. and equipment. and that these are placed in easily accessible locations in the laboratory. and repairs on metal. Provide thesis students with chemical waste bottles. b. a. 10. such as: fire extinguishers. The lab technician should: 1. e. emergency showers. Remind students to settle their lab breakage deposits immediately. Accidents resulting in medical treatment or observation should be recorded. c. wood. first aid kits. and others. f.g. fine students for failure to observe laboratory rules or inform the lab instructor of any violations to enforce penalty. and deliveries. Under the supervision of faculty member. where recommended). 3. and recommendations for the prevention of reoccurrence. Manage the stock of chemicals and materials: keep records of materials. aprons. Laboratory Operations & Safety Protocol 8 of 65 V1:2010 . first aid kits. Ensure that safety materials and paraphernalia are well in stock and replenished when used up. mechanical repairs. assist the faculty/lab supervisor in implementing proper first aid measures and if necessary assist in the proper and speedy conveyance of the injured person to a medical facility. do various operations such as purification of solvents by distillation. 6.
Report all accidents. Avoid unnecessary exposure to chemicals: a. research supervisor.1. Be informed about experimental procedures and appropriate safety concerns: a. Laboratory Operations & Safety Protocol 9 of 65 V1:2010 . This should be done even if gloves are used. Contact lenses must not be worn. No pranks. or cellphones. 4.g. d. Know the location of the fire extinguisher. Wear proper personal protective equipment: a. c. flammability. e. Consider lab work serious work. from the work area. d. and those specific to your experiment. and face before leaving the work area. reactivity. consult with your lab instructor/supervisor. books. 6. first aid kit. d.5 The Role of Students/Researchers The student/researcher is responsible for complying with the safety rules and operational procedures of the Chemistry Department. When you are organized. and how to dispose of chemical waste properly. Any deliberate behavior that may compromise you and your co-workers’ safety may be grounds for your dismissal from that lab class/course. Always wash hands. arms. or the lab technician in charge. c. smoke. drink. Aprons or some suitable protection must be worn at all times in the laboratory. Follow hazard precautions. The first thing you do in entering the lab is to put on your apron the last thing on leaving is to remove it. e. you are less likely to commit accidents. Do not perform unauthorized work. safety shower and eyewash. especially with hazardous chemicals. Never eat. the student should be responsible for their personal safety when they are working in the lab. whether major or minor. Safety glasses must be worn inside the laboratory. preparations. Wear clothing appropriate for work: Do not wear shorts. You are university students. 3. 5. If in doubt. or apply cosmetics inside the lab or storage areas. and experiments. b. having read the experimental procedure in advance. Goggles resting on your forehead are not in a position to protect your eyes. corrosiveness. Although the Department aims to provide a safe working environment for its students. Follow protocols for handling chemicals and for dealing with spills. Read and prepare for remedies in case of exposure. b. sandals. Research the hazards and physico-chemical properties of the chemicals used in the experiment. Every student should: 1. and toxicity. such as bags. or slippers. fire escape. e. horseplay. use medication. how to dispense the correct amount of reagents properly. or other acts of mischief. Read and understand general safety procedures. Never work alone. to the lab instructor. and treat all chemicals with respect.) Learn how to use instruments properly. Come in the lab prepared. Wear clothing that would protect your body against contact with chemicals. 7. c. Keep personal belongings. 2. b. Confine hair and loose clothing. Never use your handkerchiefs or face/hand towels to wipe things in the lab. not preschoolers. (See Figures 1-5. Keep a neat and clean work area.
immediately alert any of the following: • • • • The disaster-assistance team captain of the building (local The Fire Marshall at OAS. 4112. 4112. Who do you call for assistance? If the incident is small and contained. disaster-assistance team captain of the building.g. area and immediately call for help. lab technician.) as above. 2. Try to alleviate the immediate need in the situation without compromising your own safety: • • • Remove an incapacitated co-worker from the accident area Turn off fuel and power lines. or any Chemistry Dept. If the situation is beyond your control and presents a danger to you. etc. personnel (local 5624 or 5631) The Chemistry Department office (local 5620) 4111. especially the one in-charge of the lab The lab technician. evacuate the Note: For fires.3 (Fire Emergency). follow evacuation procedures under Section 2.Chapter 2 Emergency Protocols 2. immediately alert any of the following: During office hours: • • • Any faculty of the Chemistry Department. rapidly spreading fire). Alert others in the area of the accident. use fire extinguisher Immediately call for help. Gonzaga (local 5624) 5102) The University security personnel (local 4111. 4113 ) After office hours: • The University security personnel (local If the incident is out of control (e. 4113) The following fire departments: o o o o 8363) Marilag Fire Sub-station (913 0125/510 9299) Pinagkaisahan Fire Sub-station (474 2695) New Era Fire Sub-station (931 9894/931 4956) Central Fire station (928 • University personnel (faculty. 3.1 Dealing with Emergency What to do: 1. Laboratory Operations & Safety Protocol 10 of 65 V1:2010 .
2. Luke’s Medical Center UP Health Service 372 3825 924 3601 925 2401 723 0301 981 8500 local 112 Laboratory Operations & Safety Protocol 11 of 65 V1:2010 . Fire Departments • • • Quezon City Marikina Mandaluyong 928 8363 933 3076 5322189 Ateneo de Manila University • • Trunk line Telephone Operators 426 6001 local 4199 Chemistry Department Directory • • • • Department Office (Chair/Secretary) Schmitt Hall Stockroom SEC Stockroom Faculty Offices 5620 local 5624 local 5631 local local 5621-23. University Gym) Direct Line local 4100 local 4101 local 4102 local 4104 426 5940 Infirmaries within Campus • • • • Health Service HealthDev (at the Social Development Complex) Jesuit Residence (ask for Infirmary) Loyola House of Studies (ask for Infirmary) local 5110 local 4630-33 local 3399 local 3650 Nearby Hospitals • • • • • Capitol Medical Center National Kidney Institute Philippine Heart Center St. 5625-33 University Security Office • • • Main Office (North Wing.2 Emergency Telephone Numbers Note: These numbers are subject to change. and must be updated annually. University Gym) Gate 2 Gate 3 local 4111 local 4112 local 4113 local Office of Administrative Services • Fire Marshall (Gonzaga Hall) 5103 Physical Plant Office • • • • • Administrator Secretary Switchboard Operation Center (South Wing.
etc. Secure potential hazards in your area such as open flames.2.g. Close windows and doors in your area. Immediately inform any of the following: • • • 5624) The Fire Marshall at OAS. e. Do not re-enter the building unless you are given permission to do so. offer first defense against flammable liquids or electrical fires without leaving a powder residue which could harm electronic equipment. and crawl if necessary. If you encounter smoke.) To use the fire extinguisher. There are emergency exit routes posted on the wall of each room. S – Squeeze the lever. containing CO2. Provide the following information 3. 2. 5. Most chemical laboratory fire hazards require dry chemical multipurpose extinguishers (ABC) which must be installed in hallways. stay close to the floor. 1. A – Aim the nozzle. Close the doors to confine the fire. small appliances. Leave the building using the nearest stair tower to reach ground level. Evacuation Procedures. Meet the fire department outside of the building and provide information about any special hazards and other considerations. for example. b. • • • • P – Pull the pin. S – Sweep from side to side. WARNING: Do not attempt to fight a fire that is spreading rapidly or if you are not sure how to operate the fire extinguisher. (See Figure 5. 5. ovens. “Gas” extinguishers. assume that the emergency is real until you are informed otherwise. When alerted of a fire. If the fire is small and confined to its origin. Identify yourself Identify the kind of fire Identify the building and room number Give your telephone number Don’t hang up until the dispatcher has the needed information.) 4.3 Fire Emergency What to do: 1. d. Gonzaga hall (local 5103 or 5118) Quezon City Fire Department (928 8363 or 928 6535) The disaster-assistance team captain of the building (local a. remember: Fire Extinguishers. use the portable fire extinguisher. Laboratory Operations & Safety Protocol 12 of 65 V1:2010 . compressed gasses. c. (See Figure 4. 4. 3. The fire may block your exit. and evacuate the building. Alert others in the area of the fire. 2. e.
Immediate Action 1. For small liquid spills (<100 mL). Shut down all equipment and vacate the area until it is decontaminated. local 5624 or 5631). proceed with clean-up operations. Wear appropriate personal protective equipment: goggles. 9. Ventilate the spill area. (See Spills of Specific Types of Chemicals below). 3. 6. paper towels. Mix neutralizing chemical into the spill. Laboratory Operations & Safety Protocol 13 of 65 V1:2010 . Do not leave paper towels or other materials used to clean up a spill in open trashcans in the work area. Acids and Bases: Avoid contact with skin. Dispose them properly. Check for and remove contact lenses. 5. or an absorbent can be used to contain the spill. and turn off spark producing equipment. and there is no fire hazard. even of concentrated reagents. 4. respectively. Do not brush up solid spills since this may produce airborne dusts.2. If a volatile. you can add water to the spillsand mixture to act as dust-suppressant. 2. 5110). and respiratory protection. 3. Forcibly hold the eyelids open as necessary. Use. 7. If the material is not reactive to water. If the material is not particularly volatile. not highly corrosive. as appropriate. 6. paper towels are not suitable for cleaning up flammable spills. Add sand to contain the solid spill. a minimum of 20-minute flushing with copious amounts of water is recommended. and if applicable. use an absorbent material that will contain the liquids. when necessary by opening windows or use a fan. 2. Avoid skin contact and minimize inhalation. 5. Exposed skin should be thoroughly washed with soap and water. adding some water to provide solvent for the neutralizing reaction. Rotate the eyeballs so that all surfaces are rinsed.4 Chemical Spill Dropping containers or otherwise spilling chemicals in the open lab can result in exposure to hazardous agents. has a low order of toxicity. Continue flushing with water for 15 minutes or more. Use pH paper to determine whether the acid or base have been neutralized. Everyone should leave the affected area. Seek medical attention (Health Service. Notify the faculty or lab technician in charge of the class (local 4. 7. It is important to minimize the extent and possible effects of exposure to these hazardous chemicals. treat the spill like a potential health hazard by avoiding exposure and seeking assistance from a trained personnel. flammable or toxic material is spilled. For chemical splashes on the eyes. When the toxicity of the spilled material is unknown. gloves. warn everyone immediately to extinguish flames. closing the door and warning others not to enter the contaminated area. Spills of Specific Types of Chemicals 1. safety shower or eye wash fountain. sand. especially for volatile and toxic spills. Place in a labeled plastic bucket or container. Neutralize acids and bases with solid sodium bicarbonate and citric acid. Use sand/soil to contain liquid spills. However. These should be laundered separately from other clothing before reuse. Spill Clean-Up 1. Use paper towels and dustpan to collect the spill residue. Any contaminated clothing should be removed and containerized. Report the incident to faculty or lab technician. 8. A quantity of solid not much greater than the volume of the liquid spilled should be sufficient.
WARNING: A mask may protect the wearer against inhalation of noxious gases. Laboratory Operations & Safety Protocol 14 of 65 V1:2010 . 3.5 Escape of Noxious Gases Immediate Action 1. (See Figure 6. Place the source of gas in a hood (and make sure to turn the motor on). etc. Modesty should not deter removal of clothing in a true emergency. Solvents may cause the oil to dissolve in the skin. WARNING: Do not use solvents to wash off offending oily chemicals. open windows. The situations described herein are the ones of particular concern in a chemistry laboratory. evacuate personnel. 2. Forcibly hold the eyelids open as necessary. Check for and remove contact lenses. Mercury spilled into floor cracks can be made non-volatile by amalgamation with zinc dust. thus increasing the damage to the skin and promoting poisoning through the skin. Do not use sulfur to cover mercury since this will just complicate disposal. Keep victim warm. refer to a more extensive first aid manual.After neutralizing. The stockroom must also be ready to provide first aid supplies: bandage. Rotate the eyeballs so that all surfaces are rinsed. 5110. Watch victim carefully for shock. In any event. cutting or tearing them if necessary to get them off rapidly. Contaminated materials used to clean up the spill should also be placed in properly-labeled containers. Leave the room in either upright or crawling position. WARNING: Do not clean-up hydrogen fluoride (hydrofluoric acid. mopping should follow. HF) with silica-containing materials such as sand or vermiculite. etc. accordingly. etc.) Domestic vacuum cleaners must not be used. First Aid for Specific Types of Accidents 1. Chemicals on the Body: Flush areas copiously with water under safety shower or under the sink. Very much lighter vapors will rise. or by adding a mixture of finely powdered sodium thiosulfate (85 g) and powdered EDTA (15g). Final rinse is clear water. but be warned against absorption through the skin of such substances such as HCN gas. Heavy vapors (MW >>29) will lie along the floor. Rinse mop and bucket. burn ointments. 2. Chemicals in the Eyes: A minimum of 20-minute flushing with copious amounts of water is recommended. Social 2. Quickly remove chemically soaked clothing. Mercury: Because of the high toxicity of mercury vapor. aniline vapor. and the burning and poisoning through skin by HF gas. antiseptic wash. spilled mercury should be cleaned-up immediately and thoroughly using an aspirator or vacuum device. Take (do not send) the victim to the Office of Health Services (local Sciences Building). Water-insoluble oils must be washed off with soap and water. and close off the room from the rest of the building.. 2. poison remedies. 2.6 First Aid All members of the Chemistry Department must undergo a yearly first aid and cardiopulmonary resuscitation (CPR) course and should be ready to apply first aid measures to an injured person in a lab. For more general concerns.
). Only very minor burns should then receive burn ointment. Shock can be fatal! Be on the lookout for it. WARNING: Application of cold to large areas of the body produces shock. Keep moist. Keep victim warm. shivering and chills. There is always the possibility that they may collapse en route. have him lie with head lower than the body. Take (do not send) the victim to the Office of Health Services (local Sciences Building). jittery talk. charcoal.7 Medical Attention After First Aid After being administered first aid. and shallow breathing. Also refer to first aid wall charts. it seals the chemicals under the greasy coating. WARNING: Do not apply greasy burn ointment to chemical burns. Watch victim carefully for shock. It may be the most dangerous result of an accident. On most fire burns. Social Fire Burns: Rush victim under safety shower or smother fire on victim with a blanket (e. 1. The cold shower will suffice. Keep moist with neutralizing solution soaked on gauzes. etc. or better. lab coat. extreme dullness or sluggishness. cold sweat. Social 4. nausea. To restore blood to the head. Cover burned areas in 3% boric acid. as 3% boric acid (remedy for base) or 5% bicarbonate (remedy for acid). Keep victim warm. vomiting. Treatment with ice water is not recommended for large-area burns. Burned areas should be cooled immediately. Remedies include: egg albumin. are usually printed in red letters on the bottle labels. Social 2. weakness of the arms and legs. Symptoms of shock include: cold sweat. even though at the time you dismiss them they assure you they feel fine and are quite ready to go alone. Signs of Danger: Watch the victim for blood leaving the head (face turning white) or excess blood in the head (face turning red). and tannic acid. Keep victim warm. Take (do not send) the victim to the Office of Health Services (local Sciences Building). Shock: Accident victims experience shock. extreme paleness of the face.g. jacket. the injured person must be taken (not sent) directly to the Office of Health Services (local 5110. 3. and difficulty in breathing. trembling. 5110. Take (do not send) the victim to the Office of Health Services (local Sciences Building). Social Sciences Building). Soak affected part in ice water for 20 minutes to reduce deep burning. very rapid pulse.Follow water-washing with the correct neutralizing solution. The supervisor (faculty/lab technician) or a co-worker must accompany the injured person in seeking medical treatment. Keep victim warm. 5110. Chemical Poisoning: Get poison remedies/antidotes from the stockroom manager or lab technician. hence the advantage of using the safety shower. mustard powder. heavy sweating. Watch victim carefully for shock. Also. pounding heart. shallow rapid breathing. All these are signs of trouble and require immediate medical attention. and first aid precautions to follow in case of chemical poisoning. a victim should not drive a car for several hours after a severe injury. 5110. Watch victim carefully for shock. 2. have shock victim lower head between knees. Laboratory Operations & Safety Protocol 15 of 65 V1:2010 . magnesium sulfate. The antidotes required. etc. do not apply burn ointment. Watch victim carefully for shock. Transporting the Injured Person: No one should be sent to seek medical attention without accompaniment. nausea.
3. neutralizing chemicals. or sunstroke. It is the supervisor’s (faculty’s) responsibility to ensure that the student receives prompt treatment of the injury by obtaining first aid or assistance to medical treatment. or hemorrhage of the head. apoplexy. fractured skull. If he vomits. 2. 2.2) are of the utmost importance for correcting procedures. abdominal injuries. Any injury resulting in lost work time. Give oral stimulant (hot tea or coffee) if conscious. fire extinguishers. WARNING: It is still best to take the victim to see a physician first before giving any oral remedy. for establishing legal responsibility for an accident. Report the Use of Safety Equipment: Immediately report the use of eye wash. for establishing a claim for damages of insurance benefits.8 Reporting Accidents and Injuries All personal injuries and accidents should be reported in order that corrective action may be taken to minimize the probability of recurrences. It is the student’s/researcher’s responsibility to notify his supervisor immediately of any work-related injury or illness. showers. Laboratory Operations & Safety Protocol 16 of 65 V1:2010 . Only give inhalation (aromatic spirits of ammonia or amyl nitrate) in the mouth only if the victim is unconscious. a coworker should notify the supervisor as soon as possible. or medical treatment in excess of first aid will be investigated by the Chemistry Department to determine liability or the possible need for corrective action. 1. for demonstrating whether faculty/staff were negligent in preventing the accident or careless in subsequent treatment of the victim. to the building manager or lab technician so that the equipment can be recharged of replaced. hospitalization. be sure his air passages do not become blocked. It is also the supervisor’s responsibility to file an injury/accident report with the Chemistry Department. 3. File injury/accident reports with the secretary of the Chemistry Department. etc. elevate the head rather than lower it! This may occur in the case of sunstroke. Accident reports (see Appendix A. Keep the victim warm. Do not give stimulant until bleeding is controlled. and not at all in case of fractured skull. apoplexy.WARNING: If the face is flushed or red. If the injured person is not able to do so.
the Ateneo Chemistry Department has been able to maintain a good and effective working set-up which has yielded beneficial outcomes. It has given us the edge on effective equipment maintenance because malfunctions were reported at once. and its long tradition of high standards in safety. Laboratory Operations & Safety Protocol 17 of 65 V1:2010 . finances. and effort—has led to the conservation of resources. The heart of an effective working set-up is the responsibility and discipline of users of the building and its facilities. are maintained to a high level of compliance. The building is named after Fr. you will see that the good of many outweighs individual convenience. Over the years. Schmitt.1 Schmitt Hall T he Chemistry Department is housed at Schmitt Hall (formerly the Chemistry Building). This set-up is also based on as sense of trust that each one will do his share in ensuring these practices. While in some cases some convenience is sacrificed. The judicious use of resources—energy. which in the past have served the Department well. It has saved the Department from serious accidents because of the emphasis on safety at the workplace.Chapter 3 The Use of Building & Facilities 3. who was instrumental in its design and construction. It has spared us from serious theft because security measures were taken in earnest and implemented. William J. with a laboratory extension for undergraduate labs on the 2nd floor of the 3rd wing of the Science Education Complex (SEC-C).
d. Stockroom Balance Room Instrumentation Room AFM Room Multimedia Room Glassblowing Workshop 208 308 310 204 111 4th Floor 4. b. c. 2nd Floor SEC-C 307 309 6. Lecture/Seminar Rooms 3. Offices a. 2nd Floor SEC-C 209. c. Research Laboratories a. b. Department Office Faculty Offices Faculty Lounge 112 101. Organic Synthesis Lab Natural Products Lab NCIC Wet Lab Biochemistry Research Lab Polymer and Materials Lab Polymer/Biochemistry Wet Lab Electrochemistry Lab 203 202 206 304 302 305 303 Laboratory Operations & Safety Protocol 18 of 65 V1:2010 . Service Rooms a. Director’s Office Staff Office GC-MS Lab NMR Lab 102 104 103 105 5. c.2 Room Addresses 1. c. National Chemistry Instrumentation Center (NCIC) a. d. b. d.116. General Chemistry Lab Organic Chemistry Lab Analytical Chemistry Lab Physical Chemistry Lab. Teaching Laboratories a. also houses the AAS and HPLC 207.3. 301 201 109. d. b. e. b. g. 115. f. 205 2.115. f. c. e.
m. d.1). should be cleaned. It is for your own safety.m. and the Office of Administrative Services must be informed. aircon. Try to work out a schedule that will go from 6:00 a. b. Anyone staying beyond 10:00 p. The faculty lounge should be kept clean and orderly. and the sub-master key (for labs). 3. e. The building is opened to the public by the building manager by 7:00 a. faucets.3. to 10:00 p. b. natural products labs) from a faculty member. For research assistants and students doing research. d. c. c. b. so that the entry or exit from the lab is easy in an emergency. All used utensils. Always lock doors after every use.m. a. Laboratories should only be unlocked when there are lab classes or when an experiment has to be performed in that lab. faculty and staff can stay inside the building until 10:00 pm. A written request form (Appendix A. Sign at the Night Log. and doors. Tables should be wiped clean. e. 5.m. faculty or staff may open the main door and the classrooms. Sink should be properly cleaned. the opening and locking of rooms is the main responsibility of the faculty handling the class. etc. Toilet and shower room should always be maintained in a clean. lights. polymer and materials. a. Only faculty members and the lab technicians have access to keys for the main door. and locked by the utility man at 6:00 p. coffee maker. etc. The last person to leave a laboratory should make sure that the following are closed/locked/turned-off properly: windows. plates. should be submitted to the secretary of the Chemistry Department at least 2 days before the event. 4. However. gas lines (LPG. Food supplies should be sealed and arranged properly. c. Bathroom tissue should not be removed from the toilet. During these times all doors should be unlocked for safety. hoods. Lights should be turned on only in the appropriate areas of work. glasses. respectable condition. Researchers and graduate students may loan keys to the special labs (instrumentation. 3. In case the building manager is not yet around by this time. Anyone sleeping in the faculty lounge should be up by 6:30 a.. It is a basic rule in any good chemistry lab to have at least 2 people in the lab. but both the lender and Laboratory Operations & Safety Protocol 19 of 65 V1:2010 . This will allow the office to inform the administration and security. overnight stays are discouraged. N2. endorsed by the mentor/supervisor. He. As a rule. to ensure the safety of lab workers. Overnight stays in the building should be processed with the Department office: a. they have the responsibility of seeing to it that the labs used are properly locked after use. should be responsible for obtaining permission and getting access to the main door. 2.4 Access Keys 1. their respective offices.m.). a.m. If an experiment requires longer than 15 hours. b. At least two people should stay for overnight work. 2. For classes.3 Use of the Building 1. Never assume that someone else will do these for you. ovens and other lab equipment (unless overnight run is explicitly allowed by senior faculty). overnight stay needs to be approved by the mentor and department chair.
and AFM room. Review the instructions before the operation. 4. indicating the time they are returning.g. This is needed to prevent further damage and to ensure speedy repair. b. Please be considerate of faculty members who hold offices in some of these special labs. and glassblowing workshop: a. Access keys are loaned to you in trust.g. 4. Do not attempt to fix the equipment yourself. When using the balance room. 5. the physical chemistry lab. 3. As a rule. 4. and shut-down routines. Most major instruments have voltage regulators.6 Use of Instruments 1. especially the power-up. 3. When using the polymer and materials lab. 2. These cannot be moved/relocated without the explicit approval of the Department Chair. without explicit approval of the Chairperson and a senior faculty member. Laboratory Operations & Safety Protocol 20 of 65 V1:2010 . Understand the instrument thoroughly. so that no fatal mistakes are committed. Report immediately to the person-in-charge or to the Department office when instrument supplies are running low (e. the research labs. The performance of the balances are dependent on temperature. Faculty offices should never be left unlocked. Any loss of keys should be reported immediately to the Department office. they have to put up a sign on the door. etc. If they are going to leave the room for a short period of time (e. Verify the power requirements of the instruments (110 or 220V) before plugging it into the outlet and turning it on. and there are no other people using the room. 3.). they should be locked at all times. warm-up. 2. 3. If no sign is placed or if the time is over. Equipment and accessories used should be kept in proper order. anyone with a key to the room should lock it and report the matter to the Department office.)All users must sign the instrument logbook. turn on the aircon. Report any noticeable malfunction of the instrument immediately by reporting it to the Department office. When using the instrumentation room. This assumes responsibility in the use of the keys. If students are using the room and do not have easy access to the key. Do not unplug them from their voltage regulators. (The student’s name must be marked in the Instrument Authorization Table posted in the analytical chemistry bulletin board. SHORT NOTES are provided as supplement to the manuals. Return the items to where they were found. but keep them closed. 3. Creating duplicates on your own is strictly prohibited. no equipment (major nor minor) can be taken out of the building. the AFM room. Keys should never be left lying around in the rooms. either or both the aircon or the dehumidifier must be turned on (for the FTIR and the AFM). All users must familiarize themselves with the operation of the instrument.5 Special Rooms 1. Unlock the doors. A student cannot use the instrument alone unless he/she is authorized to use that instrument. c. When rooms are unoccupied. ink. chart paper. 10-15 min). they should take the responsibility of informing the faculty involved or the stockroom technicians when they are finished.the one who borrowed are accountable for the keys. Keep the doors locked even when leaving the room for a short while. 6. Access/use of any instrument requires check-in and authorization by faculty-in-charge.
The Instrument manager updates his list of students authorized to use the instrument. ON TH SPOT checking-in is not allowed. analysis. The student’s mentor must approve the student’s plan to check-in. Important Reminders and Considerations 4.7. Procedure for Instrument Check-in 1. If the instrument room is locked. During the check-in. 2. 9. date. When done. The student and faculty-in-charge schedules the next session. Only students who passed the safety exam will be allowed to use the instrument. After the last use of the instrument. 2. computer) turned on. 10. time. Procedure for instrument check-out 1. Laboratory Operations & Safety Protocol 21 of 65 V1:2010 . The faculty-in-charge signs the check-in part of the instrument authorization form as proof of check-in. water bath. 6. Instrument Parts c. The student must already have actual samples to run during check-in. instrument. For computers connected to the instrument. Safety and Precautionary Measures d. The student who wishes to use the instrument must fill-up the instrument authorization form (Form A. The date of authorization must be different from the date of check-in. For the commonly-used instruments (UV-Vis. Equipment that is left on without any note will be shutdown after 6:00 pm. make sure he/she understands that you are leaving and you are turning over the responsibility of shutting down to him/her. he/she can ask a person who is already authorized to use the instrument to analyze his/her samples. The student schedules the instrument check-in wth the faculty-in-charge one week before the actual check-in. 3. students who wish to use it must request the technician or a faculty member to open the lab for them. Only students who will be using the instrument more frequently will be checked-in. do proper shutdown. Instrument parts b. Lock the lab door immediately. instrument use has priority over other use. the student must schedule an instrument check-out with the faculty-in-charge. All graduating students must make sure that they have checked-out of the instruments they are authorized to use for clearance purposes. Instrument accessories c. the faculty-in-charge must discuss the following to the student: a. The Faculty-in-charge signs the certificate of authorization if the student has demonstrated competency in using the instrument during the next sessions. Working environment 3.3). The student submits to the instrument manager for filing and documentation the instrument authorization form filled up to the certificate of authorization part. 5. Short Notes of the Procedure e. oven. He/She can get a copy of the form from the instrument manager. Leave a note (name. comments) if you are leaving something (e. AAS and IR spectrophotometers). If the student will be using the instrument only once or twice. Consumables d. Instrument General Working Principles b. 8. a general check-in will be scheduled every July of each year. The faculty-in-charge inspects the following during check-out: a. If someone is using it.g.
Second Offense: Oral reprimand and community service. The department chairperson together with the faculty-in-charge and/or the instrument manager decides whether to grant the request or not based on the instrument schedule. He/She must include in the letter the purpose of the instrument use. If the requesting party agreed to the terms and conditions. Instrument use and b. Operator fee 4. If the request is granted. Mentor will be notified. feasibility of the analysis and availability of the needed accessories. 2. The person writes a letter addressed to the department chairperson requesting to use an instrument. Mentor will do necessary action. 3. Procedure for instrument use by outside parties 1. He/She must include as an attachment the details of the procedure and instrument conditions. Laboratory Operations & Safety Protocol 22 of 65 V1:2010 . Mentor will be notified. the department chairperson assigns to the available faculty-in-charge the granted requests. Third Offense: One week ban in using the laboratory.Penalties Any student who fails to follow the policies and guidelines will be subject to the following penalties: First Offense: Oral reprimand from the instrument manager and the department chair. the department chairperson discusses with the requesting party the department policies on the use of instrument and the charges: a.
and consumed in an area free of hazardous substances. Chemicals that are no longer needed should not be permitted to accumulate in the lab.3 Eating. arrangements should be made between individuals working in separate laboratories outside working hours to crosscheck periodically. Access to exits. Coffee. such as having two persons in the same room during a particular operation.4 Housekeeping There is a definite relationship between safety performance and orderliness in the laboratory. and cold rooms. Likewise. Drinking. if appropriate). exits. it is essential to plan for interruptions in utility services such as electricity. Operations should be designed to be safe. Waste containers must be labeled for the type of waste for which they are intended. eyewash stations. and fire extinguishers. Food should be stored. and inert gas. Work areas are to be kept clean. safety performance inevitably deteriorates. unless it is explicitly allowed by the Chair and senior faculty.Chapter 4 General Safety Principles 4. such as fire hazards (See Figure 8). security guards may be asked to check on the lab worker. biological hazards. 4. the laboratory lights should be left on and an appropriate sign should be placed on the door or near the set-up. and cosmetics is a potential route for exposure to toxic substances. it is prudent to avoid working in the laboratory alone. Standard signs and symbols have been established for a number of special situations. soft drinks. emergency equipment. Wastes are to be deposited in appropriately labeled receptacles. chemicals should not be brought into designated eating areas. are likewise not to be used for food storage.and hazard-sign systems in the lab should enable a person unfamiliar with the usual routine of the lab to escape in a emergency (or help combat it. Under normal working conditions. Never leave a set-up unattended overnight. Cleanup should follow the completion of any operation of at the end of each day. Glassware and utensils that have been used for lab operation are not to be used for food or beverages. water. The supervisor has the responsibility for determining whether the work requires special safety precautions. and Smoking Contamination of food. etc. laser operations. In any case. ice chests. snacks and lunches are not to be brought into laboratory areas. and chemicals and equipment must be properly labeled and stored. handled. Stairways and hallways should not be used as storage areas.2 Working Alone Generally. Alternatively. Extinguishers are to be labeled to show the type of fire for which they are intended. must be maintained free from obstructions. and controls.1 Overnight Operations If laboratory operations are carried continuously or overnight. Experiments known to be hazardous should not be undertaken by a worker who is alone in a laboratory. Lab refrigerators. and plans should be made to avoid hazards in case of failure. Temporary holding containers should be clearly marked. smoking materials. 4. drink.5 Warning Signs and Labels Laboratory areas that have special or unusual hazards must be posted with warning signs. 4. The safety. 4. Other signs should be posted to show the locations of safety showers. Laboratory Operations & Safety Protocol 23 of 65 V1:2010 . When housekeeping standards fall.
Gas cylinders must be used in an upright position and clamped securely at all times. 2. even though the cylinders are secured to a dolly. 4. Shields are to be placed so that all personnel in the area are protected from hazard. Acceptable methods of support include: wall-mounted or bench-mounted gas cylinder brackets. Laboratory Operations & Safety Protocol 24 of 65 V1:2010 . is prohibited. regulators. rolling. or any other oxidizable substance. Moving by spinning. a regulator for oil-pumped nitrogen could produce a serious explosion if subsequently used for oxygen.g. due to the oil residue. i. 6. A cylinder connected to a piece of equipment and properly supported is considered to be in use. or contamination.6 Shielding for Safety Safety shielding is to be used for any operation having the potential for explosion such as 1. Pressurized apparatus should have an appropriate relief device.7 Compressed Gases The following rules summarize a few of the basic guidelines for the use and storage of compressed gases: 1. 5-10 times more material). For movement within shops and laboratories. 4. If the reaction cannot be opened directly to the air. sliding. Toxic and poisonous gases must be used only in fume hoods or other enclosures vented directly outdoors. graphite.e. Compressed gas cylinders must be supported and firmly restrained at all times. whether full or empty. an apparatus that is a closed system unless it is designed and tested to withstand pressure. For example. Whenever a reaction is attempted for the first time (small quantities of reactants should be used to minimize the hazards).8 Systems Under Pressure Reactions should never be carried out in. 4. 2. may result in the rapid and violent oxidation of normally non-reactive materials. gauges. grease.4. and fittings must be scrupulously free of oil. 3. chains or belts anchored to walls or benches (see Figure 9 in the Appendix). Appropriate first aid and antidote information and supplies must be provided and clearly marked at room entrances. cylinders weighing less than 50 lbs may be carried. if desired. Whenever operations are carried out under non-ambient conditions. All oxygen valves. 7. Pressure regulators and gauges must be compatible with the cylinder valves. Smoking is not permitted in the area where flammable gases are used or stored. etc. Although oxygen is quite safe under normal temperatures and pressures. nor heat applied to. 8. 5. and 3. elevated temperatures and/or pressures. The pressure regulators must be removed and valve protection covers replaced before moving cylinders.. the use of adapters is prohibited. Gas cylinders must have the valve protection cover in place (see Figure 9 in the Appendix) except when in use. an inert gas purge and bubbler system should be used to avoid pressure build up. Whenever a familiar reaction is carried out on a larger than usual scale (e. pipes. Appropriate dollies are to be used to move cylinders weighing more than 50 lbs.
4. which can represent unusual risks for the first-time user. separatory funnels containing volatile solvents can develop considerable pressure during use. and surfaces they cool. Hand protection should be used when picking up broken glass.10 Glassware Accidents involving glassware are a leading cause of lab injuries. can cause severe burns if allowed to contact the skin. which leads to an explosion hazard.9 Cold Traps and Cryogenic Hazards The primary hazard of cryogenic materials is their extreme coldness. Wetting the glassware should also facilitate the process of inserting glass tubing into rubber stoppers. Adequate hand protection should be used when inserting glass tubing into rubber stoppers or corks or when placing rubber tubing on glass hose connections. Do not store in a refrigerator or freezer. They. Dry ice should be added slowly to a liquid portion of the cooling bath to avoid foaming over. and hands should be held close together to limit movement of glass should fracture occur. Proper instruction should be provided in the use of glass equipment designed for specialized tasks.) Glassware which is to be heated should be Pyrex or a similar heat-treated type.) Broken glassware should be disposed of in a special container marked BROKEN GLASS. Tubing should be fire polished or rounded and lubricated. The use of plastic or metal connectors should be considered. Insulated gloves and a face shield may be needed when preparing or using some cold baths. Vacuum-jacketed glass apparatus should be handled with extreme care to prevent implosions. Glass-blowing operations should not be attempted unless proper annealing facilities are available. Careful handling and storage procedures should be used to avoid breaking glassware. Appropriate insulated gloves should be used when handling dry ice. (For example. and suffocation can result. Neither liquid nitrogen nor liquid air should be used to cool a flammable mixture in the presence of air because oxygen can condense from the air. Equipment such as Dewar flasks should be taped or shielded. 4. Only glassware designed for vacuum work should be used for that purpose. Workers should avoid lowering their head into a dry ice chest: carbon dioxide is heavier than air. Laboratory Operations & Safety Protocol 25 of 65 V1:2010 . (Small pieces should be swept up with a brush into a dustpan.
The system may be accessed by any computer within the campus (http://cmis. Scanner-server synchronization Chemical data in the barcode scanner are easily synchronized with data in the server 8. PDEA and PNP System not only allows monitoring of chemicals on-stock but also controlled chemicals under the EMB.Chapter 5 Chemical Management 5. The system of tracking is container-based. students) No accounts are assigned to Level 1 users. which provide quick access to: ü Physico-chemical information ü Handling and storage conditions ü Health and safety information ü Disposal considerations 4.e. CMIS is meant to address the need for accessibility of information. consumption. 2. CAS number. Access to the different features depends on the user-levels as shown in below: User Level 1 (Guests. Supplier details and hazard codes) Laboratory Operations & Safety Protocol 26 of 65 V1:2010 . threshold reports. chemical movement) can be exported as comma-separated version (CSV) files which can be opened in Microsoft Excel. Hazardous Waste Inventory System The CMIS is linked to the Hazardous Waste Inventory System (HWIS) which allows monitoring of chemical waste generation and disposal.ateneo. Level 1 users may: 1.e classification of wastes. Each chemical bottle is provided with a tear. accurate inventory.e. Information database Supplier and chemical information are stored in the database. 3. chemical expiration. PDEA and PNP.and chemical resistant barcode sticker.edu). management and compliance with various laws. Tracking of controlled chemicals under the EMB. generation of reports (i. chemical waste disposal and chemical waste movement) User Levels CMIS has four (4) user-levels (Table 1). efficient purchasing. Open Office Spreadsheet or any text-capable reader 6. Using the CMIS. Back-up and Recovery Mechanisms CMIS has features which provide means to back-up the database and restore all data should the system fail 7. Reports are easily prepared using the system’s Reporting Service 5. Exportable Reports Reports (i. Obtain chemical information on chemicals (i. stock-level.1 Chemical Management and Inventory System (CMIS) The Chemical Management and Inventory System (CMIS) combines a web-based inventory system and barcode scanner to provide a means for efficient updating and query of chemical stock levels. Check availability of chemicals in the department 2. Safety Data Sheets (SDS) bank Safety Data Sheets (SDS) for chemicals are stored in the system. IUPAC name. Different user-levels Only selected accounts can access restricted areas of the system. Features 1.
To Request for Chemicals. If request has been approved. User Level 2 (Teaching Assistants (TAs). click on the “Click here to View Existing Chemical Request” 2. The system will then provide the requestor with an update on the status of his/her request. 2. 3. Level 2 users also have access to information regarding the stock level and stock location of the chemicals. If the chemical is on stock. 3. the system will then ask for the following: Concentration – what is the concentration of the chemical that you need? Grade – what is the grade of the chemical? How much do you need? – Input amount is in liters or grams. Provide the chemical’s common name. The system will then ask for the chemical’s common name. Users may change the password using the Password Management feature of the system. click on the “Request” Link from the Home Page. A page will appear where the Chemical Name or IUPAC Name or CAS Number of the chemical can be entered. To check the status of chemical requests. 5. Laboratory Operations & Safety Protocol 27 of 65 V1:2010 . Checking status of request 1. The Request Home Page will then be shown. 6. Stock Inquiry 1. List of requested items may also be viewed by clicking on “Click here to review requested items. To end request wizard. Show the signed form to the stockroom personnel and claim chemicals. Click on the Chemical’s Common Name link to show the information about the chemical.” 8. Please take me to check out. 2. To check for stock levels of chemicals using the Inventory System. Any special instructions for this particular chemical? – Special instruction on how the chemical is to be handled or stored should be noted here. click on “I do not need any more items.” The check-out page will then show the reference number which can used by the requestor to review status of request. Research Assistants (RAs) and junior faculty) An account and temporary password is given to Level 2 users. Requests for other chemicals can be added to current request by clicking on “Click here to add more chemical requests.3. Proceed by clicking on the “Click Here to Request for a Chemical” link. Access (Material) Safety Data Sheets ((M)SDSs) of chemicals 4. click on Stock Inquiry. Requestor Name – the name of the requesting party (who made the request?) Requestor Department / Company – the department or company affiliation of the requestor Purpose – the reason for the request (where will the chemical be used for?) Request Date – date of the request (when was the request made?) 4. In addition to the features available to a level 1 user. Request for a chemical Steps in requesting for a chemical (See Figure 11 in the Appendix for the overview) 1. 4. Fill up the form with the required information to proceed.” 7. Input the reference number issued upon checked out from the Request Wizard. 3. print the form and have it signed by your thesis mentor.
This includes management of CMIS accounts. Senior Faculty) An account and temporary password is also given to Level 3 users. A “Back-up succeeded” message will then appear on the screen. use. CMIS manager. technicianin-charge of CMIS) An account and temporary password is also given to Level 4 users. storage locations.e. Open the Sync Station software and ensure that all databases are connected. Setting up of additional data such as supplier information. Setting up of additional data to the CMIS 1. The safety officer together with the system administrator of the MIS office is in-charge of the maintenance of the database system. Click on the “Synchronize All” button to perform all operations in sequence. Laboratory managers. preparation of a back-up of the database and synchronization of the server and barcode scanner. Users may change the password using the Password Management feature of the system. To save the back-up file. PNP and PDEA) monitor the purchase. click on the “add ____” link to display the form where new entries can be supplied. Lab technicians. Level 3 users also have access to the reporting services of the system. chemicals and thresholds) on the home page. Suppliers.). supplier information. the government has started to implement various laws. right-click on the “The Back-up file” link and choose “Save Link As…” or “save Target As…” Synchronization of server and barcode scanner The sync station software is used to synchronize the barcode reader’s database with the server’s database. Level 4 users also have access to the hazardous waste inventory system and the administrative functions of the system. Preparing a back-up of the database 1. Sub-pages for the selected field will then be displayed. 4. To synchronize the server and barcode scanner. In addition to the features available to a level 3 user.User Level 3 (Department secretary. waste management. 5. storage locations. close all active application in the barcode scanner. The system will then display a dialog box once all operations are finished. encode additional data (e. Place the scanner in the dock and wait for “active sync” to finish synchronization. User Level 4 (Dean. Then click the link. Level 4 users have access to all of the features of the CMIS. Different implementing agencies (i.g. 1. add/update chemical bottle inputs. chemical brand. Click “add ____. storage. 2. rules and regulations covering a broad range of activities related to the purchase.e.2 Controlled Chemicals In 2004. dangerous drugs and explosives (Tables 2 and 3 in the Appendix). To add new data. “Back-up. consumption.” 2. click on the link ”Back-up and Recovery” link on the Administrator Functions Section. Users may change the password using the Password Management feature of the system. hazard codes and threshold levels may be done by clicking on the appropriate links (i. Department Chair. storage and disposal of the following controlled chemicals: Laboratory Operations & Safety Protocol 28 of 65 V1:2010 . EMB. In addition to the features available to a level 2 user. hazard codes. 5. thresholds etc. chemical brands.” button to submit and save new data. To back-up the databse. 2. These can be grouped under the general headings of environment. and disposal of chemicals. new storage locations. chemical grade. 3. movement.
sale. the person then writes a letter addressed to the department chairperson requesting to purchase the chemical. manufacture. upon presenting the official receipt from the Cashier. from their importation. 3. 4. The department chairperson together with the faculty-in-charge and/or the person in-charge of controlled chemicals decides whether to grant the request or not based on the amount of chemical on stock. delivery. The university is required to register its possession and use of Mercury and Cyanide compounds. Since the department uses these compounds for laboratory classes and research.EMB The EMB regulates mercury. transportation. The university is required to obtain a Purchaser’s license from PDEA. administration. cyanide and their compounds. the department chairperson approves the request. He/She must include in the letter where the chemical will be used as well as the amount of the chemical being requested. distribution. sale. the university is required to obtain a “License to Possess Explosive and Explosive Ingredients” from PNP. Requesting party proceeds to the Cashier to pay for the chemicals being purchased. possession or use of explosives and explosive ingredients such as nitrates and chlorates. This license is renewed yearly. Laboratory Operations & Safety Protocol 29 of 65 V1:2010 . Procedure for requesting for controlled chemicals by outside parties 1. If the chemical is available. 2. Semiannual reports on the purchase and consumption of PDEA-controlled chemicals are also submitted to PDEA. Monthly consumption reports are also submitted to PNP. distribution. administration. If the request is granted. transport and disposal. A separate “Permit to Purchase and Move Explosive and Explosive Ingredients” should also be obtained from PNP prior to purchase of nitrates and chlorates. possession or use of the following prohibited and regulated drugs and precursor chemicals (See Table 4 in the Appendix) Since the department uses some of these compounds for laboratory classes and research. transportation. Since these chemicals are also used in the teaching laboratories. The person checks availability of chemical using the CMIS. PNP PNP monitors and regulates the importation. delivery. use. PDEA PDEA monitors and regulates the importation. Requesting party may then pick-up the requested chemicals from the stockroom.
It is therefore wise to minimize exposure to chemicals. Irritant: a chemical. Toxic: (a) a chemical that has a median lethal dose (LD50) of 50 mg/kg but not more than 500 mg/kg body weight when administered orally to albino rats weighing 200-300 g each. benzene Laboratory Operations & Safety Protocol 30 of 65 V1:2010 . benzene. (b) a chemical that has a medial lethal dose (LD50) of 200 mg/kg body weight when administered by continuous contact for 24 hrs (or less if death occurs within 24 hrs) with the bare skin of albino rabbits weighing between 2-3 kg each. Chemicals can have local or systemic effects.Chapter 6 Handling Chemicals 6. Carcinogen: a chemical agent that causes a malignant disease or increases statistically the risk of cancer. Hepatotoxins: chemicals which produces liver damage. and agents which damage the lungs. Some of these compounds are fairly common materials used in many laboratories. sensitizers. toxic or highly toxic agents. Local toxicity refers to the direct action of chemicals at the point of contact. 7. thiourea. but causes reversible inflammatory effect on living tissue by chemical action at the site of contact. Systemic toxicity occurs when the chemical agent is absorbed into the bloodstream and distributed throughout the body. skin. 1. or 2 mg/L or less of mist. 3. Signs & symptoms: jaundice. or 2 mg/L or less of mist. Corrosive: a chemical that causes visible destruction of. liver enlargement. carbon tetrachloride. or irreversible alterations in. whether by initiating or promoting it. fume. Health hazard means a chemical for which there is statistically significant evidence based on at least one study conducted in accordance with established scientific principles that acute or chronic health effects may occur in exposed personnel. corrosives. agents which act on the hematopoietic system. (b) a chemical that has a medial lethal dose (LD50) of 200 mg/kg but not more than 1. nephrotoxins. 5. Acute effects are observed shortly after exposure. eyes. Chronic effects result from long-term exposure or appear after a latency period. Chemicals: carbon tetrachloride. and o-toluidine. (c) a chemical that has a median lethal concentration (LC50) in air of 200 ppm by volume or less of gas or vapor. neurotoxins. Highly toxic: (a) a chemical that has a median lethal dose (LD50) of 50 mg/kg body weight when administered orally to albino rats weighing 200-300 g each. Toxic effects are also classified as acute or chronic. which is not corrosive. 6. Targeted Organ Effects a. It includes chemicals which are carcinogens. hepatotoxins.000 ppm by volume or less of gas or vapor. fume.1 Hazardous Chemicals All chemicals have toxic effects at some dose level for some route of exposure.000 mg/kg body weight when administered by continuous contact for 24 hrs (or less if death occurs within 24 hrs) with the bare skin of albino rabbits weighing between 2-3 kg each. 2. living tissue by chemical action at the site of contact. or dust. dioxane. affecting one or more organs. reproductive toxins. Hazardous chemical means any chemical which is capable of causing harm to people and the environment. hydrazine. or dust. such as chloroform. when administered by continuous inhalation for one hour (or less if death occurs within one hour) to albino rats weighing between 200-300 g each. 4. Sensitizer: a chemical that causes a substantial proportion of exposed people or animals to develop an allergic reaction in normal tissue after repeated exposure to the chemical. or mucous membranes. when administered by continuous inhalation for one hour (or less if death occurs within one hour) to albino rats weighing between 200-300 g each. (c) a chemical that has a median lethal concentration (LC50) in air of 200 ppm but not more than 2. irritants. nitrosamines.
DBCP.4 oC. d. carbon disulfide. d. Flammable: a chemical that falls into the following categories: a. the total volume of which make up 99% or more of the total volume of the mixture.b. decrease in motor functions. or higher. spontaneous chemical change. Eye hazards: chemicals which affect the eye or visual capacity. Signs and symptoms: edema. when tested by the method described in 16 CFR 1500. proteinuria. f. Signs & symptoms: defatting of the skin. tightness of chest. or which can be ignited readily and when ignited burns vigorously and persistently as to create a serious hazard. flammable. yields a flame projection exceeding 18 inches at full valve opening. Agents which act on the blood or hematopoietic system: decreases hemoglobin function. a compressed gas. an absolute pressure exceeding 104 psi at 54. flammable: an aerosol that. chlorinated compounds. sterility. in a container. Aerosol. an organic peroxide. or (c) a liquid having a vapor pressure exceeding 40 psi at 37. (b) a gas or mixture of gases having. Chemicals: halogenated hydrocarbons. h. an oxidizer. acids. c. Note: flashpoint means the minimum temperature at which a liquid gives off a vapor in sufficient concentration to ignite when tested according to ASTM D 56-79. g. deprive the body tissues of oxygen. that is liable to cause fire through friction. 2. and ASTM D 3278-78. or (b) a gas that. Signs & symptoms: cough. Liquid. at an ambient temperature and pressure. pressure. Chemicals: ketones. 3. 1. or a flashback (a flame extending back to the valve) at any degree of valve opening. Compressed gas: (a) a gas or mixture of gases having. regardless of the lower limit. Gas. Reproductive toxins: chemicals which affect the reproductive capabilities including chromosomal damage (mutations). forms a range of flammable mixtures with air wider than 12% by volume. Signs & symptoms: conjunctivitis. asbestos. Chemicals: carbon monoxide. Signs & symptoms: cyanosis. Nephrotoxins: chemicals which produce kidney damage. unstable (reactive) or water-reactive. when tested by the method described in 16 CFR b. at ambient pressure and temperature.8 oC.45. in a container.8 oC or higher. c. pyrophoric. Agents which damage the lung: chemicals which irritate or damage pulmonary tissue. regardless of the pressure at 21. flammable: any liquid having a flashpoint below 37. and irritation.8oC as determined by ASTM D-323-72. corneal damage. forms a flammable mixture with air at a concentration of 13% by volume or less. almost instantaneous release of pressure.2 oC. Cutaneous hazards: chemicals which affect the dermal layer of the body. or high temperature. Signs & symptoms: narcosis. shortness of breath. Laboratory Operations & Safety Protocol 31 of 65 V1:2010 . cyanides. and effects on fetuses (teratogenesis). absorption of moisture. Neurotoxins: chemicals which produce their primary toxic effect on the nervous system. behavioral changes. but below 93. loss of consciousness. and heat when subjected to sudden shock. rashes. or retained heat from manufacturing or processing. uranium. Chemicals: organic solvents. Signs & symptoms: birth defects. except any mixture having components with flashpoints of 93. ASTM D 93-79.3 oC.3 oC. the total of which make up 99% or more of the total volume of the mixture. Explosive: a chemical that causes sudden. Solid flammable: a solid other than a blasting agent or explosive. Physical hazard means a chemical for which there is scientifically valid evidence that it is a combustible liquid. explosive. except any mixture having components with flashpoints of 37. Chemicals: mercury.1 oC. Chemicals: lead. Combustible liquid: any liquid having a flashpoint at or above 37. A chemical shall be considered to be a flammable solid if. Chemicals: silica. 4. an absolute pressure exceeding 40 psi at 21. e. gas.8 oC. flammable: (a) a gas that.
Laboratory Operations & Safety Protocol 32 of 65 V1:2010 . 8. Spills and splash can result in overt contamination of the skin. Pyrophoric: a chemical that will ignite spontaneously in air at a temperature of 54. Many of the particulates generated during such procedures do not settle out but remain suspended in the air and are carried about by air currents in the room. Water reactive: a chemical that reacts with water to release a gas that is either flammable or presents a health hazard. but solids and non-volatile liquids can also present an inhalation hazard for laboratory personnel. by local action. Actually. pouring. or dusts can produce poisoning by absorption through the mucous membrane of the mouth. 2. throat. or equipment which become contaminated curing experimental activity. 6. decompose. eyes. stirring. or as produced or transported. Chemicals can also gain entrance into the body when contaminated hands touch the mouth. sores.44. The main portals of entry for chemicals through the skin are the hair follicles. where one or both of the hydrogen atoms has been replaced by an organic radical. laboratory personnel may unconsciously contaminate themselves when they touch work surfaces. or cuts. 9. A common result of skin contact is localized irritation or dermatitis.1500. sebaceous glands.4 oC or below. or will become self-reactive under conditions of shocks. nose. However. 1. which facilitate the absorption of chemicals into the body. Laboratory chemicals in the form of dusts and particulates can become airborne when transferred from one container to another. thereby causing fire either of itself or through the release of oxygen of other gases. Inhalation. that initiates or promotes combustion in other materials. pressure or temperature. Inhalation hazards are often associated with gases and volatile chemicals. 5. Also. For many operations it is not obvious that an aerosol is being generated and laboratory personnel may not be aware that a hazardous situation exist. it ignites and burns with a self-sustained flame at a rate greater than one-tenth of an inch per second along its major axis. its solubility in tissue fluids. Oxidizer: a chemical other than a blasting agent or explosive. Alert laboratory personnel will take care not to create unnecessary aerosols. pipeting. Unstable (reactive): a chemical which in the pure state. 7. mists. The follicles and glands are supplied with blood vessels. its concentration. injections with a needle and syringe. condense. sweat glands. 3. Inhalation of toxic vapors. and cuts or abrasions of the outer layers of the skin. and lungs and can seriously damage these tissues. glassware. Such operations include weighing. and which may be considered to be a structural derivative of hydrogen peroxide. and removing caps and stoppers. Grinding and crushing procedures can also produce aerosols. Inhaled gases or vapors may pass rapidly into the capillaries of the lungs and be carried into the circulatory system. and the duration of exposure. all laboratory operations involving an open vessel will result in the release of an aerosol. a number of materials are absorbed through the skin to produce systemic poisoning. One of the most frequent exposure to chemicals is by contact with the skin. gases.2 Routes of Exposure Dermal Contact. Organic peroxide: an organic compound that contains the bivalent —O-O— structure. 6. will vigorously polymerize. Some of these particulates are capable of being inhaled and deposited in the respiratory tract. The degree of injury resulting from inhalation of toxic substances depends on the toxicity of the material. Splash created from spills and during vigorous shaking and mixing also results in aerosol formation.
containers of toxic chemicals may break resulting in hazard from contact with broken contaminated glass. vapor. “Contact lenses should not be worn in the chemistry laboratory Laboratory Operations & Safety Protocol 33 of 65 V1:2010 . Accidents involving needles and syringes can results in injection contamination through the skin. Full-face shields that protect the face and throat should always be worn when maximum protection from flying particles and harmful liquids is needed.g. A face shield or mask may be needed when a vacuum system (which may implode) is used or when a reaction that has a potential for mild explosions is conducted. contact lens use should be restricted. The needle and syringe is one of the most hazardous items used in the laboratory. nose bridge width. safety glasses should be worn with face shields. Furthermore. passed flammability tests. Three dimensions that are important in providing a comfortable fit include: temple length. and lens-retaining frames. 6. It is the responsibility of the lab supervisor to determine the level of eye protection required and to enforce eye-protection rules. The eyes are of particular concern because they are so sensitive to irritants. The practice of mouth pipeting can result in aspiration of toxic materials. The minimum acceptable eye protection requires the use of hardened-glass or plastic safety spectacles. Also. and must be worn only in conjunction with approved safety eyewear. goggles should be worn when working with glassware under reduced or elevated pressure and when glass apparatus is used in combustion or other high-temperature operations. 4. Face Shields: Goggles offer little protection to the face and neck. Ocular exposure can occur via splash or when contaminated hands rub the eyes. Other eye protection should be worn when a significant splash hazard exists. and visitors in laboratories where chemicals are stored or handled. when the work environment entails exposure to chemical fumes. or highly particulate atmosphere. more than two hours per day). 1. intense heat. Contact Lenses: Contact lenses do not provide eye protection in the industrial sense. molten metals. and intense light sources. They are intended for wear when there is danger of splashing chemicals or flying particles. and lens diameter. For example. impact resistance. For full protection. The American Chemical Society’s Committee on Chemical Safety states in Safety in Academic Chemistry Laboratories that. Splash goggles (“acid goggles”) that have splash –proof sides should be used when protection from harmful chemical splash is needed. Impact-protection goggles have screened areas on the sides to provide ventilation and reduce fogging of the lens and do not offer full protection against chemical splashes. 3. The type of safety device required will depend on the nature of the hazard and the frequency with which it is encountered.3 Protective Clothing and Equipment Eye and Face Protection. Safety glasses: Ordinary prescription glasses do not provide adequate protection from injury to the eyes. identification of contact lens wearers should be ensured for appropriate emergency care and for protection in work areas hazardous to the eyes. There are specific goggles and masks for glassblowing. These should have: a minimum thickness of 3 mm. In addition. Few substances are innocuous in contact with the eyes and a considerable number are capable of causing burns and provide for rapid absorption of many chemicals. Food items and utensils themselves can become contaminated when stored in the laboratory. Ingestion of toxic materials in the laboratory can also occur when contaminated hands come in contact with the mouth of with food items which are placed in the mouth. Side shields that attach to regular safety spectacles offer some protection from objects that approach from the side but do not provide adequate protection from splashes.Ingestion. welding. such as lasers and ultraviolet light. 2. Injection. Safety spectacles are recommended for those who require eye protection frequently and/or for long durations (e. Goggles: Goggles are not intended for general use. faculty. Eye and protective devices must be worn by students. or splashes. staff. Ocular Exposure.
” Masks may be used to minimize exposure to noxious fumes. Before each use.g. however. Gloves should be selected on the basis of the material being handled. and butyl and natural rubbers. Contact lenses can also trap particulate foreign matter in the eye and thereby produce abrasion of the cornea. Laboratory coats and aprons are intended to prevent contact with dirt and the minor chemical splashes or spills encountered in lab-scale work. These materials differ in resistance to various substances. Insulated gloves should be used when working at temperature extremes. a known carcinogen. punctures. the particular hazard involved. which are self-contained breathing apparatuses with their own air tanks. if significantly contaminated by them. It is best not to use gloves made either entirely or partly of asbestos. or torn clothing and unrestrained long hair. Respiratory Protection. and may itself present a hazard (e. Lab workers should know the appropriate techniques for removing protective apparel. but they are by no means total respiratory protection as air masks. Proper gloves should be worn whenever the potential for contact with corrosive or toxic materials and materials of unknown toxicity. Clothing and Protective Apparel. or become ensnarled in apparatus and moving machinery. Use of Gloves. Furthermore. However.e. and for similar operations where protection from chemicals is not needed. personal clothing that will be worn home should be covered by protective apparel. combustibility) to the wearer. he is warned against absorption through the skin of such substances as HCN gas. Common glove materials include neoprene. and very hot or very cold materials. the removal of the contact lens to achieve immediate irrigation is made nearly impossible by involuntary spasm of the eyelid… Gases and vapors can concentrate under the lenses and cause permanent eye damage. Plastic or rubber aprons provide better protection from corrosive or irritating liquids but can complicate injuries in the event of fire. Various synthetic materials such as Nomex and Kevlar can be used briefly up to 538 oC. skimpy. and tears. gloves should be inspected for discoloration. shoes. gloves should be washed. Before removal. they can be used safely for limited time periods if specific use and glove characteristics (i. if the material is impermeable to water. only contact lenses worn for therapeutic reasons can be permitted in the laboratory. Laboratory Operations & Safety Protocol 34 of 65 V1:2010 . Chemical spills on leather clothing or accessories (watchbands. The clothing worn by lab workers can be important to their safety. especially any that has become contaminated. Furthermore. should be removed immediately. dip into chemicals. for inserting glass tubes into rubber stoppers. and synthetic materials such as Nomex or Tyvek are satisfactory. Coats and aprons do not significantly resist penetration by organic liquids and. Skimpy clothing offers little protection to the skin in the event of chemical splash. and their suitability for the operation being conducted. belts) can be especially hazardous because many chemicals can be absorbed in the leather and then held close to the skin for long periods. Loose or torn clothing and unrestrained long hair can easily catch fire. It is the responsibility of the lab supervisor to determine whether specialized hand protection is needed for any operation and to ensure that needed protection is available. If the possibility of chemical contamination exists. capillary action tends to hold the offending liquid under the contact lens and against the surface of the cornea. Such personnel should not wear loose. polyvinyl chloride. are primarily a protection for clothing. thickness and permeation rate and time) are known. plastic aprons can accumulate considerable static charge and should be avoided in areas where flammable solvents could be ignited. sharp edged objects. For these reasons. Double gloving is recommended when handling highly toxic or carcinogenic materials.except for therapeutic reasons… In the event of a chemical splash into an eye. Finger rings can react with chemicals and also should be avoided around equipment that has moving parts. Glove materials are eventually permeated by chemicals. Leather gloves may be used for handling broken glassware. rayon and polyesters are not. although the wearer is protected against inhalation exposure. nitrile. Such items must be removed promptly and decontaminated or discarded. In that event. These. etc. Cotton (pure or 60:40 cotton-polyester). aniline vapor.
places a physical barrier between the workers in the lab and the chemical reaction. or cloth sneakers should not be worn in labs or areas where mechanical work is being done. Rapid mixing of liquids with pipets by alternate suction and expulsion or forcibly expelling material from a pipet should be avoided. carrying. Eating. Other items and equipment which become contaminated during experimental activity should be decontaminated with the appropriate solvent. etc. Chemical fume hoods are the primary containment device in the laboratory to control airborne contaminants generated during experimental procedures. Access to laboratories which have toxic chemicals should be controlled. if dropped.Foot Protection. 6. Use of Chemical Fume Hoods. Safety shoes are required for personnel whose job duties require the lifting. smoking. These should be placed in properly-labeled waste containers for disposal. Floors should be cleaned with a wet mop or with a vacuum cleaner equipped with a HEPA filter. Procedures involving volatile chemicals and those involving solids or liquids that may result in the generation of toxic aerosols should be conducted in a chemical fume hood rather than on the open bench. which. Personnel should wash their hands immediately after completion of any procedure involving toxic chemicals and when they leave the lab. Dropping the contents from a height generates greater aerosol. 2. The use of liquid soap dispensers is recommended. Minimizing Aerosols. or allowing the contents to run down the wall of the receiving vessel. or storage of utensils. Decontamination of Work Surfaces. Placing a reacting chemical system within a hood. or moving. the laboratory worker should develop techniques which will minimize the creation of aerosols: 1. Laboratory Operations & Safety Protocol 35 of 65 V1:2010 . drinking. This not only protects those people who might otherwise enter the lab. This barrier can afford workers protection from chemical splash. Dry sweeping or dry mopping contaminated laboratory floors could re-aerosolize contamination. and minor explosions. application of cosmetics. or against lacerations from sharp edges. of objects weighing more than 15 lbs. fires. but also reduces interruptions to the lab worker which could lead to accidents. Shoes should be worn at all times in laboratories or other areas where chemicals are used or stored. Personnel Practices. 4. sandals. Chemical fume hoods provide personnel protection by means of directional airflow from the laboratory into the hood through the face opening. Work surfaces should be protected from contamination by using disposable plastic backed absorbent paper or stainless steel trays. Safety shoes are used to protect the feet against injuries from heavy falling objects. Care should also be taken when discarding contaminated cloves or plastic backed absorbent paper used to cover the work surface so that contamination is not aerosolized in the process. The laboratory door should be kept closed while experiments are in progress. would likely result in a foot or toe injury. especially with the hood sash closed. Discharge fluids from pipets as close as possible to the fluid level of the receiving vessel. against crushing by rolling objects. 3. Since all procedures involving an open vessel of liquids or powders generate aerosols. This airflow reduces the potential for escape of airborne contaminants into the laboratory. Mechanical pipeting aids are to be used for all pipeting procedures.4 Handling Toxic Materials Access Control. sprays. or food containers should not be allowed in labs where toxic materials are used. Perforated shoes. food. The practice of mouth pipeting should also not be allowed. chewing of gum.
5. Verify airflow PRIOR to doing an experiment by attaching a Kimwipe or ribbon to the sash. Each vacuum service. Stock quantities of chemical carcinogens are to be stored in designated storage areas. 6. Experiments should be planned so that. Storage vessels containing chemical carcinogens are to be first placed in an unbreakable outer container before being transported to lab work areas. The outer container is to be labeled both the name of the chemical carcinogen and the warning: CAUTION – CHEMICAL CARCINOGEN.5 Handling Carcinogens Procedures for handling toxic materials also applies to chemical carcinogens. Chemical carcinogens Laboratory Operations & Safety Protocol 36 of 65 V1:2010 . and should include the dates of acquisition and disposition. Working Quantities. Face velocities between 100 to 120 feet per minute are acceptable for most uses. The primary container must be placed in a durable outer container before being transported. a separate vacuum pump or other device placed in an appropriate chemical fume hood should be used. Storage vessels containing working quantities should be labeled: CAUTION – CHEMICAL CARCINOGEN.The following practices should be observed when using fume hoods: 1. Use of Analytical Instrumentation. Work should be carried out as far back in the hood as possible. all of the materials needed for a procedure are present in the hood to eliminate disruption of airflow by carrying equipment in and out during a procedure. The storage areas should be posted with signs bearing the legend: CAUTION – CHEMICAL CARCINOGEN Authorized Persons Only. 3. Keep sash as low as possible. Contaminated materials must either be decontaminated by procedures that decompose the chemical carcinogen. Disruptive room air currents should be minimized by avoiding traffic near fume hoods and opening and closing doors near fume hoods while experiments are in progress. and Identification. Inventory. Hood work areas should be clear of unnecessary equipment and materials which can disrupt airflow and block vents. When a sample is removed from the analytical instrument. 8. Analytical instruments. is to be protected with an absorbent or liquid trap and a HEPA filter to prevent entry of any chemical carcinogen into the system. and their access controlled. Quantities of chemical carcinogens present in the work area should be kept to a minimum. or be removed for subsequent disposal. Decontamination. as much as possible. Laboratory Transport. when used with chemical carcinogens. vapors or aerosols produced by these instruments should be captured through local exhaust ventilation at the site of their production. 4. An inventory of stock quantities should be maintained. 2. Quantities should not normally exceed the amounts required for use in one week. The procedures outlined in this section are additional precautions in dealing with carcinogenic materials. Storage vessels containing stock quantities should be labeled: CAUTION – CHEMICAL CARCINOGEN. Protection of Vacuum Lines. When this is impossible. it should be placed in a tightly-stoppered sample tube or otherwise safeguarded from contaminating the lab. 7. When using a volatile carcinogen. Hoods should not be used for storage of chemicals. Storage. including water aspirators. 6. Contaminated materials which are transferred from work areas to disposal areas must first be placed in a closed plastic bag or other suitable impermeable and sealed primary container. are to be placed entirely within a chemical fume hood. Analytical equipment that becomes contaminated should not be used until it has been completely decontaminated. Check efficiency of the hood by measuring its average face velocity regularly. Use equipment with legs.
and of the appropriate procedures top use in handling such substances. heating mantles. Flammable substance should be handled only in areas free of ignition sources. dust accumulations. This possibility should be recognized. especially when materials are stored or disposed of. oil baths. Prevention of fires and explosions requires knowledge of the flammability characteristics (limits of flammability. 2. Flashpoint: the minimum temperature at which a liquid gives off a vapor in sufficient concentration to ignite when tested according to ASTM D 56-79. and phosphorous. and hot surfaces. When flammable materials are used in the lab. For example: A steam line or a glowing light bulb may ignite carbon disulfide (ignition temperature 80 oC). and burning rates) of combustible materials likely to be encountered under various conditions of use. and burn or to explode flammable materials varies with the specific type of substance. finely divided pyrophoric metals. Diethyl ether (ignition temperature 160 oC) can be ignited by the surface of a hot plate. static-generated sparks should be avoided by bonding and use of ground straps. Flammable vapors from massive sources such as spillages have been known to descend into stairwells and ignite on a lower story. Metal lines and vessels discharging flammable substances should be properly bonded and grounded to discharge static electricity. lighted matches. and special note should be taken of ignition sources lower than that at which the substance is being used. Spontaneous Ignition or Combustion: this takes place when a substance reaches its ignition temperature without the application of external heat. Materials susceptible to spontaneous combustion include oily rags. A means for assuring adequacy of clean-up should be provided. The degree of the fire hazard also depends on the ability to form combustible or explosive mixtures with air. and hot air baths. water baths. The rate at which different liquids produce flammable vapors depends on their vapor pressure. 3. Properties of Flammable Substances. If the path of vapor with the flammable range is continuous. Handling. ignition requirements. 1. When transferring flammable liquids in metal equipment. 2. Ignition Temperature (Auto-ignition temperature): the minimum temperature required to initiate or cause self-sustained combustion independent of the heat source. There are a number of potential sources of spark. The basic precautions for safe handling of flammable materials include the following: 1. organic materials mixed with strong oxidizing agents (such as nitric acid. This is of special concern when working in a dry environment such as an air-conditioned room or a room with a dehumidifier. Flammable substances should never be heated by using an open flame. permanganates. or heat in labs which can ignite flammable substances: open flames. and ASTM D 3278-78. Sources of Ignition. The vapors of flammable liquids are heavier than air and capable of traveling considerable distances. and persulfates). The possibility of spontaneous combustion should be considered. chlorates. A flammable liquid does not itself burn. 3. ignite. alkali metals such as sodium and potassium. When nonmetallic containers (plastic) are used. Preferred head sources include steam baths. for instance wipe tests or fluorescence tests. flame. Laboratory Operations & Safety Protocol 37 of 65 V1:2010 . close attention should be given to all potential sources of ignition in the vicinity. the bonding can be made to the liquid rather than to the container.which have spilled out of a primary container so as to constitute a hazard must be inactivated in situ or should be absorbed by appropriate means for subsequent disposal. peroxides. ASTM D 93-79. 6.6 Flammable Materials The ability to vaporize. Many common laboratory solvents and chemicals have flashpoints that are lower than room temperature. the flame will propagate itself from the point of ignition back to its source. it is the vapors from the liquid that burn. static electricity.
light. rubber. thermostat) removed or exteriorized. Fire extinguishers are labeled according to their fire fighting proficiency and safety in extinguishing various types of fires. Storage Rules 1. 2. When a flammable liquid is withdrawn from a drum. fire. more recently. For easy identification. Electrical Equipment: Fires in wiring. formation of substances of greater toxicity.4. Flammable liquids must not be stored in domestic type refrigerators. Fire Extinguishers. volatilization of toxic or flammable substances. Ventilation is one of the most effective ways to prevent the formation of flammable mixtures. 5. C. 5. and violent polymerization. Containers of flammable liquids shall not be drawn from or filled within buildings unless provision is made to prevent the accumulation of flammable vapors in hazardous concentrations. pressurization in closed vessels.7 Handling Explosive/Reactive Materials The large number of different classes of chemicals potentially present in a research laboratory poses increased risks from accidental hazardous chemical reactions or explosions. dispersal of toxic dusts/mists/particles. 6. containing Halon 1211 or CO2. Flammable liquids must be stored so that accidental contact with strong oxidizing agents (such as permanganates or chlorates) is avoided. 3. 6. Flammable Liquids: Fires in solvents and other flammables liquids require dry chemical. Laboratory Operations & Safety Protocol 38 of 65 V1:2010 . 4. or CO2 extinguisher labeled C. explosion. Flammable liquids must not block lab aisles or exits. and. offer first defense against flammable liquids or electrical fires without leaving a powder residue which could harm electronic equipment. B. and many plastics require water or dry chemical type extinguisher labeled A. Metals: Combustible metals such as magnesium and sodium require special extinguishers labeled D. pictograms are used to indicate the type of fire on which an extinguisher is to be used. or D. Use refrigerators that have electrical contacts (door switch. solubilization of toxic substances. These are identified accordingly: 1. Ordinary Combustibles: Fires in paper. “Gas” extinguishers. wood. when they are inadvertently mixed as chemical waste. heated in open containers. 2. Using the “wrong” type of extinguisher on a fire can endanger the user and make the fire worse. An exhaust hood should be used whenever appreciable quantities of flammable substances are transferred from one container to another. or when a drum is filled. Flammables stored in the open in the laboratory work area shall be kept to the minimum necessary for the work being done. Keep flammable liquids away from heat and direct sunlight. Most chemical laboratory fire hazards require dry chemical multipurpose extinguishers (ABC) which must be installed in hallways. both the drum and other equipment must be individually electrically grounded and bonded to each other. Halon. Hazardous reactions may cause any one or more of the following: heat generation. formation of flammable gases. They may result when incompatible chemicals are accidentally spilled. 3. A hazardous reaction occurs when two or more incompatible chemicals result in an undesirable or uncontrolled reaction with adverse consequences. labels A. cloth. formation of toxic vapors. Halon or CO2 extinguisher labeled B. or handled in any other way. energized electrical equipment and other electrical sources require a dry chemical. fuse boxes. or when they are unwittingly combined during experimental procedures. allowed to stand in open containers. 4. formation of shock or friction sensitive compounds.
If there is an accident. Incompatibility of chemicals is the prime reason for not storing chemicals on the shelf alphabetically. It is prudent to check for incompatibility wherever a change is made in chemical procedures. the disaster is compounded by the adverse reaction.It is easy to fall into the trap of becoming complacent with certain chemicals used everyday with certain procedures. Laboratory Operations & Safety Protocol 39 of 65 V1:2010 .
or otherwise render the waste nonhazardous. neutralize. Non-Halogenated Flammable Solvents. or suspected carcinogenic. diluted to non-flammable concentrations. detoxify. Only trace quantities of oils associated with cleaning and washing operations should be released to the sanitary sewer. Examples of halogenated solvents include methylene chloride. Liquids with a flashpoint less than 60 oC. toxic. liquids which corrode steel at a rate greater than 6. chloroform. The fewer the number of chemicals associated with a waste. Corrosivity. Halogenated solvents must not be combined with flammable nonhalogenated solvents.5. Acids and Bases. Disposal of solvents to the sanitary sewer is limited to low-toxicity solvents. Non-halogenated flammable solvents may be sent to the incinerator and must be free of heavy metals and reactive materials. A waste stream generated from a laboratory procedure should not be combined with other chemical wastes.1 Waste Characteristics Hazardous wastes are classified according the following: 1. absorption of moisture. Toxicity. teratogenic. If this is not practical. It is encouraged that laboratories consider ways of reducing the volume of waste or preserving the reuse of the materials through the redesign of experiments. Ignitability.g. solids capable of burning vigorously and persistently after ignition through friction.5. mutagenic. Normally unstable and undergoes violent changes. there must be careful consideration about which wastes can be combined. 7. Laboratory Operations & Safety Protocol 40 of 65 V1:2010 . miscible in water. or greater than or equal to 12. Halogenated Solvents. before final disposal. carcinogenic. forms potentially explosive mixtures with water of pH conditions between 2 and 12. reacts violently with water. Reactivity. 2. oxidizers. capable of detonation or explosive reaction. 3. Small volumes of acids and bases can be diluted and discharged in the sanitary sewer.3 Handling Specific Wastes Individual Waste Streams. or spontaneous chemical changes at standard temperature and pressure. inhalation LC50 of 2 mg/L (rats). and carbon tetrachloride. Oils. Fatal to humans in low doses. These should be converted to sulfide.35 mm per year at 55 oC. Heavy Metals.Chapter 7 Waste Disposal 7. but followed by flushing with copious volumes of water. the more economical is the disposal method for that waste.2 Waste Reduction Minimizing chemical waste generation also minimizes safety hazards. or dermal LD50 of 200 mg/kg (rabbit). which is less soluble. e. sodium metal. less than oral LD50 of 50 mg/kg (rats). Aqueous solutions with a pH less than or equal to 2. Efforts should be made to decontaminate. 7. Different waste materials should be segregated whenever possible. Recyclable materials should be kept separate from other waste. Acids and bases containing heavy metals must not be disposed to the sewer system. 4.
Glass collection containers must preferably be stored in rubber safety carriers to protect against breakage and spillage. Filled-up waste bottles should be submitted to the stockroom for signing of clearance at the end of the school year Safety Carriers. Special Handling Considerations. Laboratory Operations & Safety Protocol 41 of 65 V1:2010 . The terms most appropriately describing the waste should be included.5 Waste Containers Containers. the approximate pH of the waste should be indicated beside “Corrosive” on the label. pasteur pipettes. A bottle with a barcoded label will be issued by the stockroom personnel to the student. giving the container size and indicating whether it is full. Corrosive materials with pH less than 5.Sharps. If metal or plastic containers are used. All hazardous characteristics of waste should be identified. should be noted. 4.4 Labeling Each container must be labeled with the following information: 1. 7. etc. Carcinogenicity. Materials that may create a fire or explosive hazard.6 Disposal to Sewerage System Avoid discharges to the sanitary sewer of the following: 1. 2. and solid waste in pounds or kilograms. or teratogenicity should be specified beside “Toxic”. to caution the handler. 4. Any toxic or reactive hazards. If the waste is an acid or a base. Obtain waste bottles for chemical wastes from the stockroom 2. Procedure for obtaining chemical waste bottles 1. Each compound in the waste containers must be listed by its complete chemical name. 3/4 full. be sure that the container material is compatible with the waste. Amount. fire. including but not limited to syringes. and the approximate percentage of each compound. Principal Constituents. Sharps. razor blades. Substances or conditions which could result in an explosion. Gallon-sized four-liter glass reagent bottles are generally the most convenient. 7. capillary tubes and scalpels should be placed in puncture-resistant waste collection containers prior to disposal. or flammable gas generation should be explained. mutegenicity. 3. Waste Characteristics. Liquid waste is to be given in gallons or liters. 7. heat generation. 2. Do not use abbreviations. The amount of waste. Provide the following information: • Name • Chemical waste classification • Chemical wastes to be kept in the bottle • Name of mentor 3.
Solid or viscous materials in amounts to obstruct flow or interfere with operations. NH4 • Oxides: Mg. Cu • Chlorides: Ca. K. Sr. Mg. the containers should be labeled and marked as “Non-hazardous. the following nonhazardous materials may be placed in ordinary trash bins for disposal: Agarose Alumina Aluminum oxide Ammonium phosphate Calcium carbonate Calcium oxide Calcium phosphate Calcium sulfate Citric acid Dextrin Glycine Magnesium carbonate Magnesium chloride Magnesium sulfate Potassium carbonate Potassium chloride Potassium sulfate Sephadex Silica gel Sodium chloride Sodium citrate Sodium phosphate Sodium sulfate Stannous oxide Starch Sugars Titanium oxide Materials listed below in quantities up to about 100g or 100 mL at a time are suitable for disposal down the drain while flushing with excess water Organic Chemicals • Alkanols with fewer than 4 carbons • Aliphatic aldehydes with fewer than 5 carbons • Amides with fewer than 5 carbons • Aliphatic amines with fewer than 7 carbons • Carboxylic acids with fewer than 6 carbons • Esters with fewer than 5 carbons • Proteins. Si. NH4 • Borates: Na. K. nucleic acids. However. In general. Mg.3. 4. carbohydrates. K. Non-hazardous wastes may be disposed of as ordinary trash. Heat discharges which will inhibit biological activities or increase the waste water treatment effluent about 40 oC.7 Disposal of Chemical Wastes Non-hazardous Chemical Wastes 1. K.” 2. soluble fats and their precursors Inorganic Chemicals • Sulfates: Na. 7. Mg. Ca Laboratory Operations & Safety Protocol 42 of 65 V1:2010 . Ca. Mn. Co. Sr. 5. Ti. Non-hazardous wastes in liquid form may be disposed of down the drain. Fe. Na. or contamination of sludge of effluent from the wastewater treatment. NH4 • Carbonates: Na. Ca. Mg. Ca. Sr. Al. Discharges of any toxic material in volume of strength to cause interference with waste treatment processes.
These wastes will be hauled by DENR-accredited transporters for treatment and disposal. Laboratory Operations & Safety Protocol 43 of 65 V1:2010 .Hazardous chemical wastes Hazardous chemical wastes should be placed and segregated into leak-proof barcoded waste bottles following the classification of the DENR-EMB (See Table 5 in the Appendix).
mask. face shield or other splatter guard) for anticipated splashes or sprays of infectious materials to the face when agents must be handled outside the BSC (biosafety cabinet). From time to time. 8. 6. The lab apron or coat must be laundered every two weeks. 3. only mechanical pipetting devices are permitted. Do not pipette by mouth. spray a solution of 70% alcohol (usually ethanol) on the gloves to disinfect. University of Wyoming.Fire extinguisher/blanket Laboratory Operations & Safety Protocol 44 of 65 V1:2010 . Lab apron must be worn at all times. 5. Do not wear contaminated gloves outside the lab. Persons wearing contact lenses should also wear eye protection. Samples must be properly labeled to immediately identify the organism present. (Refer to Table 6 of the Appendix) 3. 4. First aid kit b. 7. remove and leave lab apron or coat in the lab. 8. Food is stored outside the work area in cabinets or refrigerators designated specifically for that purpose. Remove and change gloves when overtly contaminated or when torn or punctured.1 General Information Most of the procedures are adapted from Environmental Health and Safety. There are designated refrigerators (Biochemistry and Room 103 NCIC labs) for short-term storage (2 months or less) and a cryogenic container (Rm 103 NCIC lab) for long-term stock storage. Do not wash or reuse disposable gloves. 2. Always wash hands after removing gloves and before leaving the lab. there are four levels as specified by US Department of Health and Human Services and National Institutes of Health. The secondary container should be sealable and non breakable. fungi. Store the samples in these designated places only to avoid spreading and contamination of bio-free chemicals and solutions.Launceston Campus 1. the "primary" container should be carried within an outer "secondary" container.Chapter 8 Biological Samples: Handling and Waste Disposal 8. Princeton University. and yeast. Always wear mask and put on gloves in handling samples. University of Tasmania .3 Transport When transporting samples to or from the laboratory. 8.2 Storage 1. 2. Before using any of these samples. When leaving the lab.4 Handling 1. virus. Only organisms under BSL 1 are allowed in the laboratory. and Microbiology Safety and Staff Induction Manual. For bacteria specimens. know the biosafety level (BSL) of the organism first. Microorganisms refer to organisms of microscopic or sub-microscopic sizes such as bacteria. Wear appropriate face protection (goggles. 2. Familiarize yourself with the location and operation of the following emergency items for each laboratory. a.
splashes and the production of aerosols. Always use heat safety gloves when removing items from the pressure cooker. this will include a laboratory apron/coat. A heat sensitive indicator (autoclave tape) should be used in every load. Adjust the working height of the stool so that the worker's face is above the front opening. 9. call the instructor immediately. To prevent accidents. Always wear safety glasses when opening the pressure cooker. This applies particularly to the flaming of the bacteriological loop: the loop should be drawn gradually from the cooler to the hotter parts of the flame. Exits 8. 11. 5. 10. or brought into the laboratory without permission of the instructor or laboratory in-charge. If you have an accident of any kind. 11. if the cabinet is not left running. Laminar Flow Cabinet/Biosafety Cabinet (BSC) 1. indicate the date. 4. Work at a moderate pace to prevent the air flow disruption that occurs with rapid movements. 8. 3. Disinfect work surface with 70% alcohol or other suitable disinfectant.15 minutes prior to use.5 Laboratory Equipment Autoclave 1. Carry out procedures so as to minimize the risks of spills. Princeton University) 1. It is housed in the isolation room in C305. 7. The working area should be wiped with disinfectant at the beginning and end of the laboratory session. 9. Working in a BSC (adapted from Environmental Health and Safety. make sure that the door of the isolation room is closed to minimize the disruption of the airflow. cover spill with paper towel and pour on disinfectant.Eyewash station e. wait until the pressure drops to ~5 psi before opening the exhaust valve. 3. Place items into the cabinet so that they can be worked with efficiently without unnecessary disruption of the air flow. and gloves. 6. For minor spills. and sample/product protection. 2. mask. Wipe the bottom and side of the hood surfaces with disinfectant when work is completed. Delay manipulation of materials for approximately one minute after placing the hands/arms inside the cabinet. put on gloves. No slides or cultures are to be taken from. Consider the materials necessary for the planned work in the cabinet. One pressure cooker is designated for sterilization purposes while another is solely used for decontamination. environmental. Do not disturb the airflow by covering any of the grillwork with materials. 2. 4. The lid should only be open when the pressure is zero. time and contents of the cycle. Always wash your hands before leaving the laboratory. Laboratory Operations & Safety Protocol 45 of 65 V1:2010 . The BSC in the laboratory is a Class 2 cabinet designed to provide personnel. Wear appropriate personal protective equipment. A laminar flow cabinet is also available in the Biochemistry lab. The standard autoclave cycle used in this laboratory is 15 psi for 15 minutes. Gas isolation switch d. In the logbooks of either pressure cooker. Minimize the frequency of moving hands in and out of the cabinet. 5. 2. Leave for 10 minutes and then mop up. 10. 12. Before using the equipment. 8. Turn the cabinet on for at least 10 . At a minimum. 6. working with materials from the clean to the dirty side.c.
For example. 3. and is infection likely to be acquired by the respiratory route? The physical nature of the spill. the following steps must be considered. The container must be covered by aluminum foil. culture loops. 2. (adapted from Environmental Health and Safety. Handle broken glassware with brush and dustpan. Liquid Decontamination • • • Add liquid chlorine bleach to provide a final 1:10 dilution Let stand at least 20 minutes Discard down the drain Surface Decontamination • • Wipe with 1:10 dilution of chlorine bleach. others) must be prepared. Sterilization is done using the pressure cooker designated for decontamination purposes. 8. shear. or forceps – not directly with hands. tongs. and water or as thorough as sterilization. The materials must be decontaminated immediately. After decontamination. recap.6 Waste Disposal 1. Princeton University) Assessing a Biohazard Spill The biological nature of the spill. consummable items. All glassware. Do not bend. put the glassware and other materials directly touched by culture media or contaminated with microorganism in a box or leave them inside the BSC.7 Clean-up Procedure 1. A decontamination procedure can be as simple as clean-up with a solution of detergent. bleach. After the microbial laboratory activity. liquid wastes are thrown down the drain while solid wastes are thrown in trash bins. if possible. 4. For example.8. A container for used items (pipet tips. Clean the working area using surface decontamination procedure. In the event of spill incidents. preferably made of glass or other autoclave-compatible materials. or has a container been dropped. 3. The standard decontamination cycle used in this laboratory is 15 psi for 15 minutes. or Wipe with 70% alcohol 1. or smashed in some way with a greater potential for the generation of aerosols? Laboratory Operations & Safety Protocol 46 of 65 V1:2010 . pipet. break. Place full containers in an autoclave bag and sterilize before disposal in waste boxes. or remove used needles from disposable syringes or otherwise manipulate such units by hand before disposal. 2. has the spill resulted from a container knocked over on a bench with low potential for the generation of aerosols. Dispose of needles and syringes in the puncture resistant container provided in the laboratory for this purpose. 2. within the day or the day after. how pathogenic are the organisms contained or likely to be contained in the spill. and other materials used in the microbial activity must be decontaminated.
Do not pour disinfectant directly onto the spill as this may create additional aerosols. Wipe over general area again with paper towel dampened with disinfectant. vaginal secretions. 1. Wash the hands thoroughly with hot water and soap. 6. Always wear gloves in dealing with body fluids. Major biohazard spills Generally considered to be spills of major risk with larger volume and considerable production of splashes and aerosols. Dispose the gloves by sealing them in a plastic bag and placing it in a trash bin. warn others of spill and all must leave the room immediately. 2.The volume of liquid spilt. Don’t forget to wear gloves. Remove and replace any contaminated protective clothing. gloves. 4. 3. Do not re-enter the room until a minimum of 30 minutes has elapsed. 4. Notify laboratory in-charge(preferably) or other faculty member The clean up team should all don "gowns. 1. 5. Close doors and place a "DO NOT ENTER" sign on the door. and oral or nasal secretions.8 Body Fluids Body fluids include blood. 4. 8. Clean and disinfect the area with freshly prepared 1:10 (bleach: hot water) solution. Lay down absorbent material wetted with disinfectant over the spill and allow to sit for 10 minutes. vomit. If hands have been contaminated. After 10 minutes. 3. Put on gloves. Determine the extent of the spill: pour disinfectant around the edge of the spill and allow it to run "into the spill". 3. In the event of spillage. 2. 10 ml broth culture of E. face masks and safety glasses" before entering the spill area. 5. Remove gloves and wash hands. Dispose the absorbent material by sealing them in a plastic bag and placing it in a trash bin. coli that has been knocked over on the bench would be considered a "minor biohazard spill" whereas a 500 ml broth containing S. 6. Discontinue working in the immediate area. Treat all body fluids as if they are infectious. 8. 5. pneumoniae that has been dropped onto the floor would be considered a "major biohazard spill". Laboratory Operations & Safety Protocol 47 of 65 V1:2010 . Use disinfectant to wipe over areas around the spill that are likely to have been contaminated with splashes and aerosols. Lay paper towels wetted with disinfectant onto the spill and leave for 30 minutes. 8. feces. 9. urine. Hold breath. first wash hands with strong detergent followed by generous spray of 70% alcohol. put an absorbent material over the fluid. Minor biohazard spills Generally considered as a spill of minimally hazardous material with low potential for generation of aerosols. 2. mop up spill and place contaminated materials into autoclave bag. semen. 7. Remove any contaminated clothing and wash any contaminated body surfaces. 7. 1. For example.
fill-in. At least two people should stay for overnight work. These require you to fill in the form below and seek final approval from the Department Chair. Photocopy duplicates (for the Department and for yourself). Any injury resulting in lost work time. It is the student’s/researcher’s responsibility to notify his supervisor immediately of any work-related injury or illness. and submit to the secretary of the Chemistry Department. As a rule.m. is considered overtime.m. and submit to the instrument manager of the Chemistry Department. as long as there are still faculty in the building to let you out of the building. a co-worker should notify the supervisor as soon as possible.. you may work until 10 p. The FORM A. Photocopy duplicates (for the Department and for yourself).2 Form for Reporting Lab Accidents All personal injuries and accidents should be reported in order that corrective action may be taken to minimize the probability of recurrences. to 10:00 p. Work that will go beyond 10 p. fill-in.m. or medical treatment in excess of first aid will be investigated by the Chemistry Department to determine liability or the possible need for corrective action. Try to work out a schedule that will go from 6:00 a. Overtime forms may be processed within the day of the scheduled overtime. and for yourself). overnight stay shall be considered. while overnight forms must be handed in to the Department secretary at least 2 days before the event. Laboratory Operations & Safety Protocol 48 of 65 V1:2010 . fill-in. It is also the supervisor’s responsibility to file an injury/accident report with the Chemistry Department.3 Instrument Authorization Form Students who wish to use an instrument in the department are required to be checked-in first by the faculty-in-charge. The FORM A.m.1 in following pages serves as the Overtime/Overnight Request form. The FORM A.Appendices A. overnight stays are discouraged.1 Form for Overtime or Overnight Lab Work Schmitt Hall is opened to the public by the building manager by 6:00 a. Photocopy triplicates (for the Department. work that will go beyond 12 midnight is considered overnight. File injury/accident reports with the secretary of the Chemistry Department.2 in following pages serves as the Accident Report form.m. and submit to the secretary of the Chemistry Department. and locked by the security guard at 10:00 p. If the injured person is not able to do so. It is the supervisor’s (faculty’s) responsibility to ensure that the student receives prompt treatment of the injury by obtaining first aid or assistance to medical treatment. If an experiment requires longer than 15 hours. If your supervisor allows it.3 serves as the Instrument Authorization form. This will allow the office to inform the University administration and security. A. A. hospitalization.m. the Physical Plant.
get the following signatures: (print name and sign) Your Signature Your Supervisor Building Manager Chair Note: Trplicate! Laboratory Operations & Safety Protocol 49 of 65 V1:2010 .1 : Overtime/Overnight Request Full Name (please print) Date of Request Nature of Request (encircle one) Overtime Overnight Time of Event Date of Event Name of Accompanying Person(s) Signature(s) List of Special Equipment. Chemicals. After filling-in the above items. and Methods Reason: State why this work cannot be done during regular building hours.FORM A.
FORM A.2 : Accident Report
Full Name (please print) Date of Report
Lab Class and Section
Time of Accident
Date of Accident
Name of Injured Person(s)
List of Equipment & Chemicals Involved
Narrate the accident chronologically and in detail. Supply all the pertinent facts.
What first aid or medical attention were applied?
After filling-in the above items, get the following signatures: (print name and sign) Your Signature Witness 1 Witness 2 Chair
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FORM A.3 : Instrument Authorization Form
Ateneo de Manila University School of Science and Engineering Department of Chemistry
INSTRUMENT AUTHORIZATION FORM Student:____________________________________ Instrument:_____________________ Name of Mentor:_____________________________ Mentor’s Approval:______________ Purpose of Instrument Use: ___________________________________________________ __________________________________________________________________________ Waiver: I have read and I fully understand the “Policies on the Use of the Department’s Facilities and Equipment.” I _______ agree to follow the guidelines and procedures _______ do not agree to follow the guidelines and procedures because:______________
INSTRUMENT CHECK-IN Date of Check in: ________________________ Faculty in-charge: ________________________ ____Instrument General Working Principles ____Instrument Parts ____ Safety and Precautionary Measures ____ Short Notes of the Procedure ____ Important Reminders and Considerations Remarks:____________________________________________________________________---------------------------------------------------------------------------------------------------------------AUTHORIZATION CERTIFICATE Date: __________________________________ Faculty in-charge: ___________________________ I hereby certify that Mr./Ms. _____________________________ is authorized to use the ____________________________________. Limitations of use:_______________________________________________________________ -------------------------------------------------------------------------------------------------------------------------INSTRUMENT CHECK-OUT Date of Check out:____________________ Faculty in-charge: _________________________ ____Instrument Parts ____Instrument Accessories ____Consumables ____Working environment
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Figure 1: Lab benches for General Chemistry Lab. Each bench has its own locker, equipment set, hood, sink, power and gas lines, and broom for sweeping up broken glass.
2: Fume hoods for (A) the Analytical Lab, and (B) General Chemistry Lab. The fume hoods are located either on the sides of the laboratory rooms (A), or individually, for each lab bench (B).
Figure 3: Power, gas,
and water lines. These are color coded as follows: RED for power lines, GREEN/BLUE for water lines, and YELLOW for gas lines. The valves are also similarly color coded.
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B Figure 6: Aspirator set-up for the clean-up of mercury spills. A Figure 5: with exits also in all Floor plans directions to fire (red arrows) are placed prominently rooms.Figure 4: Fire extinguishers (A) and showers (B) are placed prominently in all labs. as shown here in the Organic Lab (A) and General Chemistry Lab (B). Laboratory Operations & Safety Protocol 53 of 65 V1:2010 .
Occupants are requested to be familiar with the location of the push button nearest their office/lab. Figure 8: GHS Pictograms and Hazard Classes Laboratory Operations & Safety Protocol 54 of 65 V1:2010 .Figure 7: The new (March 2004) fire alarm system in Schmitt Hall: Breaking the cover and pushing the fire alarm button triggers the alarm. There are several throughout the building.
Figure 10: Logical computer hardware setup Laboratory Operations & Safety Protocol 55 of 65 V1:2010 .Figure 9: Compressed gas tanks tethered properly and capped. Note the dolly on the right for proper transportation of gas cylinders.
Figure 11: Overview of CMIS Requesting Procedure Laboratory Operations & Safety Protocol 56 of 65 V1:2010 .
Rule or Regulation RA 8294: Regulation on explosives and materials used in the manufacture of explosives (1997) RA 6969: Toxic Substances and Hazardous and Nuclear Wastes Control Act (1990) DAO 92-29: Implementing Rules and Regulations of RA 6969 RA 8749: Philippine Clean Air Act (1999) DAO 00-81: Implementing Rules and Regulations of RA 8749 RA 9275: Clean Water Act (2004) DAO 05-10: Implementing Rules and Regulations of RA 9275 EMB Water quality management in all water bodies. storage and disposal of toxic chemicals and hazardous and nuclear wastes Air emissions. formulation and application of standards for the transport and disposal of effluent. List of laws. sewage and septage offsite. rules and regulations which govern various aspects related to chemicals. CMIS User-levels CMIS Feature Access to chemical information Access to storage locations Access to stock levels Setting up and modification of data Requesting services Chemical request management Password management Reporting services Administrative functions Access to Hazardous Waste Inventory System (HWIS) 1 x User-level 2 3 x x x x x x x x x x x x 4 x x x x x x x x x x x 1-Guest: for undergrad and grad students. development of guidelines for re-use of wastewater etc. Regulation on the use. RAs. EMB Implementing Agency Philippine National Police Coverage Restriction on purchase of nitrates. nitric acid. technicians. movement. ban on incineration Laboratory Operations & Safety Protocol 57 of 65 V1:2010 . technician-in-charge of CMIS Table 2. etc. senior faculty 4-Administrators. 2-TAs. Law.Tables Table 1. lab managers. junior faculty 3-Department secretary. Environmental Management Bureau (EMB) Chemical waste management of toxic and hazardous wastes (See Appendix). Abatement and control of pollution water bodies.
transportation. DAO 00-02: Chemical Control Order for Asbestos EMB DAO 04-01: Chemical Control Order for Polychlorinated Biphenyls (PCBs) EMB Table 4. 1-Phenyl-2-propanone 11. Importation. This includes proper segregation. Rule or Regulation DAO 97-39: Chemical Control Order for Cyanide and its Compounds Implementing Agency EMB Coverage Importation. PCB articles and PCB packaging in commercial buildings and facilities. Piperidine 8. treatment and disposal of solid waste. This also includes the use and possession by electric utilities and suppliers and the generation. manufacture. administration. Importation. sanitary landfills. transport. Ergometrine 5. formulation of guidelines and targets for solid waste avoidance and volume reduction and setting up of controlled dumpsites. Sulfuric acid 9. Methyl ethyl ketone 6. transport. Ephedrine 4. Anthranilic acid 3. Regulation of the importation. use and distribution of cyanide and cyanide compounds. Hydrochloric acid 5.RA 9003: Ecological Solid Waste Management Act (2000) DAO 01-34: Implementing Rules and Regulations of RA 9003 EMB Management of solid wastes. distribution and use of PCBs. Department of Health (DOH) Table 3. List of PDEA-controlled chemicals 1* 1. storage. sale. Implementing Rules and Regulations for specific substances Law. delivery. Phenylacetic acid 7. Norephedrine 10. storage. Isosafrole 7. Piperonal 12. 3. PCB equipment. Treatment. storage and disposal of cyanide-bearing or cyanide contaminated wastes. N-Acetylanthranilic acid 3. non-PCB equipment. storage and disposal of asbestos-containing materials or asbestos containing wastes. manufacture. distribution. transfer. PCB-contaminated equipment. Ethyl ether 4. Ergotamine 6. and other waste depots. collection. Toluene Laboratory Operations & Safety Protocol 58 of 65 V1:2010 . possession or use of prohibited and regulated drugs and precursor chemicals RA 6425: Dangerous Drugs Act (1972) Philippine Drug Enforcement Agency (PDEA). Lysergic acid 8. Potassium Permanganate 2* 1. sale. treatment and disposal of PCB wastes. manufacture of asbestos. Acetone 2. Acetic anhydride 2. Treatment.4-Methylenedioxyphenyl-2-propanone 9.
5 Potash with pH = 12.0 mg/L based on analysis of an extract Includes all wastes with a total As concentration > 5.0 mg/L based on analysis of an extract B201 B202 B203 B204 B205 B206 B207 B208 B299 C301 C302 C303 C304 C305 C399 D401 D402 D403 D404 D405 D406 Laboratory Operations & Safety Protocol 59 of 65 V1:2010 . Alkali wastes Caustic soda Potash Alkaline cleaners Ammonium hydroxide Lime Slurries Other alkali wastes D. Table 5.0 Phosphoric acid with pH = 2.13.0 Acid wastes other than B201 to B208 with pH= 2.5 Alkali wastes other than C301 to C306 with pH = 12.0 Organic acid with pH= 2.5 Includes all wastes with a total Se concentration > 1.0 Caustic soda with pH = 12. Wastes with inorganic chemicals Selenium and its compounds Arsenic and its compounds Barium and its compounds Cadmium and its compounds Chromium compounds Lead compounds Sulfuric acid with pH = 2.0 mg/L based on analysis of an extract Includes all wastes with a total Cd concentration > 5.0 Mixture of sulfuric and hydrochloric acid acid with pH = 2. Pseudophedrine 14.0 Hydrochloric acid with pH = 2. DENR Classification of Hazardous Wastes Class A: Wastes with cyanide Wastes with cyanide Description Waste Number A101 Waste containing cyanide with a concentration >200 ppm in liquid waste B: Acid wastes Sulfuric acid Hydrochloric acid Nitric acid Phosphoric Acid Hydrofluoric acid Mixture of sulfuric and hydrochloric acid Other inorganic acid Organic acid Other acid wastes C.0 mg/L based on analysis of an extract Includes all wastes with a total Ba concentration > 100.5 Ammonium hydroxide with pH = 12.5 Lime slurries with pH = 12.0 mg/L based on analysis of an extract Includes all wastes with a total Pb concentration > 5.0 Nitirc acid with pH = 2. Safrole *The salts of the substances in the list whenever the existence of such salts is possible (The salts of hydrochloric acid and sulfuric acid are specifically excluded).5 Alkaline cleaners with pH = 12.0 Other inorganic acid with pH = 2.0 mg/L based on analysis of an extract Includes all wastes with a total Cr concentration > 5.0 Hydrofluoric acid with pH = 2.
It is a cyanide (CN) or sulfide (S) bearing wastes. metal carbonyls. 2. 4. 3. It forms potentially explosive mixtures with water. Refer to CCO Wastes containing the following chemicals: antimony and its compounds. It is normally unstable and readily undergoes violent change without detonating. it generates toxic gases.5 can generate toxic gases. vapors and fumes in a quantity that poses a danger to human health D407 D499 E. Reactive chemical wastes Oxidizing agents E501 Reducing agents E502 Laboratory Operations & Safety Protocol 60 of 65 V1:2010 . tellurium and its compounds. vapor or fumes in a quantity sufficient to present a danger to human health.5 can generate toxic gases. It is normally unstable and readily undergoes violent change without detonating. It forms potentially explosive mixtures with water. When mized with eater. which when exposed to pH conditions between 2 and 12. vapor or fumes in a quantity sufficient to present a danger to human health.continuation (Table 5) Mercury and mercury compounds Other wastes with inorganic chemicals Includes all wastes with a total Se concentration > 0. zinc compounds. It reacts violently with water. It reacts violently with water. It is a cyanide (CN) or sulfide (S) bearing wastes. When mized with eater. thallium and its compounds. copper compounds. inorganic fluorine compounds excluding calcium fluoride Includes all wastes that are known to contain oxidizing or reducing agents in concentration that cause the waste to exhibit any of the following properties: 1. 3. 4. which when exposed to pH conditions between 2 and 12.2 mg/L based on analysis of an extract. beryllium and its compounds. 2. vapors and fumes in a quantity that poses a danger to human health Includes all wastes that are known to contain oxidizing or reducing agents in concentration that cause the waste to exhibit any of the following properties: 1. it generates toxic gases.
benzene. cresole. n-butyl alcohol.isobutanol. toluene. it also includes all still bottoms from recovery of these solvents and solvent mixtures. driers. cresylic acid.1. nitrobenzene. non-halogenated solvents: Xylene. carbon tetrachloride. chlorobenzene. tricholorethylene. ethyl ether. Other mixed Includes all aqueous-based wastes that also meet one or more of the subcategories.hexanol. and stabilizers containing Chromium and Lead. chlorinated flouro-carbons if they contain a total of 10% or more (by volume) of one or more of the above before use. acetone. soaps. or 2) capable of detonation or explosive decomposition at a temperature of 20 o Celsius and Pressure of 1 atm. 1. Non-halogenated organic solvents Includes the ff. ethyl benzene. pyridine. Laboratory Operations & Safety Protocol 61 of 65 E503 E599 F601 F602 F603 F610 F699 G703 G704 V1:2010 . F:Inks/Dyes/Pigments/Paint/Latex/Adhesives/Organic sludge Aqueous based Includes all aqueous based wastes that also meet one or more of the subcategories Solvent based Includes all solvent based wastes that also meet one or more of the subcategories Inorganic pigments Includes all wastewater treatment sludge from the production of inorganic pigments Ink formulation Includes all solvent washings and sludge.2 Trichloroethane.1. G. methanol. Waste organic solvent Halogenated organic solvents Includes the ff. Carbon disulfide. cyclo. 2-ethoxy ethanol and 2 nitropropane and other non-halogenated organic solvents if they contain a total of 10% or more (by volume) of one or more of these solvents before use. Highly reactive chemicals Includes all other wastes that exhibit any of the properties described for D501. Trichloroethane. spent halogenated solvents: Tetrachloroethylene. methylene chloride. and D503. 1.2.continuation (Table 5) Explosive and unstable chemicals Includes all wastes that are 1) capable of detonation or explosive reaction when subject to a strong initiating source or when heated under confinement. caustic washings and sludge or wastewater and sludge from cleaning of tubs and equipment used in the formulation of ink from pigments. D502. methyl isobutyl ketone. it also includes all still bottoms from recovery of these solvents and solvent mixtures. ethyl acetate.
all waste from facilities that process an average of 2500 fowls or more. sub-categories M504 and M505. and the chemicals listed in the Priority Chemical List. All wastes from commercial slaughter houses that slaughter an average of 500 or more animals per year . Includes all establishments that generate an average of 50 kg per day Includes all wastes from establishments that generate. all waste from poultry farms with an average of 5. and L.continuation (Table 5) H. Putrescible/Organic Wastes Animal/abattoir waste Grease trap wastes from industrial or commercial premises I. transport or treat more than 200 L of waste oil per day except vegetable oil and waste tallow H801 H802 I101 J: Containers Containers previously containing toxic chemical substances Waste containers that used to hold the toxic chemical substances listed in Classes A. Oil Waste oils Includes all wastes from animal feed lots containing an average of 100 or more animals. D. Wastes whose hazardous substances are physically immobilized by consolidation to reduce the surface area of the wastes in order to meet the waste acceptance criteria Wastes whose hazardous substances are chemically immobilized through chemical bonds to an immobile matrix or chemical conversion to meet the waste acceptance criteria Wastes whose hazardous substances are physically immobilized by enveloping the waste in a non-porous. J201 K: Immobilized Wastes Solidified wastes and polymerized wastes K301 Chemically fixed wastes K302 Encapsulated wastes K303 Laboratory Operations & Safety Protocol 62 of 65 V1:2010 . E. Containers that used to contain Polychlorinated biphenyl (PCB) are categorized as L406 and excluded from this sub-category. impermeable material in order to store hazardous wastes until such time that a proper disposal facility is available.000 fowls or more.
organs. L401 Ozone depleting substances L402 PCB wastes L406 M: Miscellaneous Wastes Pathogenic or infectious wastes Includes pathological wastes (tissues. endrin. mirex.1. fetuses. Recovered coolant containing chlorofluoro carbons (CFCs) or halons Wastes contaminated with PCB and waste products containing PCB. hexachlorobenzene. toxaphene.continuation (Table 5) L: Organic chemicals Wastes with specific nonhalogenated toxic organic chemicals Non-liquid waste containing the following: . infectious wastes and sharps Wastes containing friable asbestos. and DDT. Waste pesticides other than M505. heptachlor. chlordane. Refer to CCO. Includes all wastewater sludge from production of pesticides other than those listed in M505. Waste pesticides listed in the Stockholm Convention (POPs Convention) such as aldrin. bloods and body fluids). dieldrin.Tri-butylin .2-diphenylhydrazine benzene Waste chlorofluoro carbons (CFCs) and halons. Waste blue and brown asbestos fibers. Expired pharmaceuticals and drugs stocked at producers and retailers’ facilities. M501 Friable asbestos wastes M502 Pharmaceuticals and drugs M503 Pesticides M504 POPs (Persistent Organic Pollutants) pesticides M505 Laboratory Operations & Safety Protocol 63 of 65 V1:2010 . Refer to CCO.
Summary of Recommended Biosafety Levels for Infectious Agents1 Laboratory Operations & Safety Protocol 64 of 65 V1:2010 .Table 6.
Chemical Hygiene Plan. Memo to the Loyola Schools Community. 7 March 2006.acs. 7.org) Access Date: 6 June 2002. Health and Safety Office. 2 May 2001. Chemistry (http://www. Nestor S.gov/OshDoc/) Date Accessed: 6 June 2002. Dr. 23.Launceston Campus.labsafety. Chemical Management and Inventory System Manual.html) Date Accessed: 6 June 2002. 16. University of Wyoming. Dr. Researchers. Staff. 40 Steps to a Safer Laboratory (http://www. Laboratory Operations & Safety Protocol 65 of 65 V1:2010 . 19.S. Dr. OSHA Fact Sheet of Hazardous Chemicals in Labs (http://www. School of Science and Engineering). National Research Council Recommendations Concerning Chemical Hygiene Laboratories (http://www.osha. 11. Fabian M. 3. 5 August 2002. Dayrit (Dean. 6 August 1979. University of Illinois.References 1. in 18. Microbiology Safety and Staff Induction Manual. General Chemistry Lab (Ch 8) Course Outline & Lab Policies. Job description for Technical Assistant IV. 2. 24. 2004 21. University of North Carolina at Chapel Hill. 10. Laboratory Safety Manual. What To Do When Handling Body Fluids. 20. February 2000. Guide to Minimizing Waste in Laboratories (http://membership. Anna Miren Gonzalez-Intal (VP Loyola Schools).org/hotartcl/chas/97/mayjun/con. January 2010. Proposal.osha. Contact Lenses and Chemicals (http://pubs. Ma. Health Hazard Definitions (Mandatory) –1910. February 1992. 14.org) Date Accessed: 6 June 2002. Undergraduate Teaching Lab Safety Contract Sample (http:// www.1200 App A (http://www. Francis Ted Limpoco. University Chemical Management Plan.labsafety.com) Access Date: 6 June 2002. Safety Practices in the Department of Chemistry. 10 September 1996. 9. 1997 Policies and Standards for B.flinnsci. Frequently Asked Safety Questions (http://www. “Hazardous Waste Management” DENR AO 36. on Fire and Evacuation Drill on Friday.htm). Access Date: 14 June 2010. 13. 20 July 1987. Assunta C. Memo to the Chemistry Faculty. University of North Carolina at Chapel Hill.acs. Princeton University. Procedural Manual Title III of DAO 92-29.org) Access Date: 6 June 2002. 4. Students.osha. School of chemical Sciences. Judith Wormal. 2008 5.gov/pls/oshaweb/) Date Accessed: 6 June 2002.org/c/ccs/pub_9. Ateneo de Manila University.labsafety. Occupational Safety and Health Administration (OSHA) (http://www. Hazardous and Toxic Substances. on Operating Practices. 9 August 2002. Research and Teaching Assistants.osha. Ana M. Javellana (Chair). 15. 12. 17. 22.edu/sites/ehs/biosafety/ biosafetypage/section4.gov/SLTC/hazardoustoxicsubstances/) Date Accessed: 17 June 2010. University of Tasmania . on Safety Policies. Dr. Environmental Health and Safety (http://web. Less is Better. Memo to the Chemistry Faculty. 6.gov/pls/oshaweb/) Date Accessed: 6 June 2002.princeton. Cuyegkeng (Chair). Valera (Chair). 8. June 2002.htm) Date Accessed: 21 November 2002.
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