Cellular Respiration

AP Biology Lab #3

Janice Chan

Period 2

Partners: Kevin Ta,

What is water potential? y A solute¶s relative concentration. With diffusion and osmosis. The higher the concentration. including the transportation of ³vitally important nutrients and compounds without expenditure of excess metabolic energy. with different solute concentrations on either side of the membrane« In osmosis. 5. and. the faster the water will flow through the membrane. it restricts certain materials from passing freely through the membrane. 2. Water always moves from an area of higher water potential to an area of lower water potential.´ y The selectively permeable membrane has different cellular concentrations on either side. 4. What is the relationship between solute concentration. with transpirational pull. and glucose to pass freely. ³the absorption and early transport of water into the root system of plants. neutrally charged molecules. and the water potential of a solution? y Pressure potential and osmotic potential assist water potential in reaching equilibrium. Pre-lab Questions 1.Janice Chan Dr.´ Osmosis and its impact on plant cells allows for. ³small. such as oxygen. What are the mechanisms of diffusion and osmosis and their importance to cells? y Diffusion is known as the movement of molecules from a higher concentration to a lower concentration. Osmosis is a special type of diffusion in which the action occurs. helps transport water in the xylem. carbon dioxide. . so the osmosis moves the solution from lower concentration to higher concentration. the water moves from a region of low solute concentration to a region of high solute concentration. pressure potential. water. What are the effects of solute size and concentration gradients on diffusion and osmosis between two solutions separated by the membrane? y The solute size does not affect the diffusion of the membrane. What are the effects of a selectively permeable membrane on diffusion and osmosis between two solutions separated by the membrane? y When you have a membrane that separates two solutions.´ The mechanisms are important to cells because they allow for many life functions of the cell. ³in water separated by a selectively permeable membrane. 3. the membrane allows certain. Fuller AP Biology ± Period 2 30 September 2010 AP Biology Lab #3 ± Cellular Respiration I. but the concentration gradient does.

y .Solute concentration equals water potential. and equilibrium has been reached. water potential is 0. 6. and when the pressure potential is equal to the osmotic potential. What is the relationship of molarity to osmotic concentration? y As molarity increases. so does osmotic concentration.

their cellular respiration will increase. Remove sensor. PASCO Xplorer GLX with CO sensor and temperature sensor 2. Press start button twice. Press start button on Xplorer to begin data display. Remove dry peas from bottle. If there is light coming from a heat lamp shining down on the peas. Rid all CO that may be present. If germinating peas are cooled from room temperature to 10 degrees C. every minute for 15 minutes. Record concentration of CO every minute for 15 minutes. Cellular Respiration Objective a. . vi. Fill 500 ml half beaker half full with water and add ice while stirring. replace CO sensor. ii. 25 soaked [germinating] pea seeds 4. drain CO again. Materials 1. Place temp sensor next to bottle. Part A i. until temp reaches 10 C. To measure the conditions that affect the rate of cell respiration. Record again. vii. then their respiration will decrease. iv. If peas are made to leave dormancy and germinate. restart data collection. be sure that jar is sealed by stopper on sensor. Ice V. b. Place 25 dry peas into CO bottle. v. II. 25 dry pea pods 3. III. Procedure a. invert open bottle and shake for several seconds. Place 25 soaked peas into bottle. 500 ml beaker 5. c. insert CO sensor into bottle. IV. iii. then their respiration will increase. remove soaked peas [place on paper towel] and invert and shake open bottle.Cellular Respiration Lab I. Hypothesis a. Title a.

2 2923 24. regulating temperature at 10 Orange = With light. iv. Red = Soaked. Record again every minute for 15 minutes.0 3372 33.2 805 28.6 656 26 25 1280 22. Blue = With ice.6 1020 10.6 2870 33 24.0 2080 33.9 833 10.2 2714 24 1142 9.2 1181 10. VI.9 24.6 2048 22.3 2604 24 1091 9.7 507 23.9 1147 30.viii.7 1900 22.8 1711 22.8 741 10.4 791 10.4 24.0 1870 33 24.9 565 10.5 1370 31.9 903 10.6 25 1029 22. Key i.0 3645 31. iii. press again twice to restart data collection.8 417 22.1 24. Using germination and dry peas. Time [ Minutes] Concentration [PPM] Temp [ C] Concentration [PPM] Temp [ C] Concentration [PPM] Temp [ C] Concentration [PPM] Temp [ C] 11 12 13 14 15 407 543 543 544 543 546 541 542 545 546 551 552 544 543 540 545 24.7 25 732 22. 1 Black = Original.8 2389 23.6 484 10. Return soaked peas to bottle.7 964 30 24.8 24.4 24. [Concentration & temp] Add ice as needed to keep temp as close to 10 C as possible.3 2818 24.8 3115 34 24.8 . ii. create and carry out own experiment to explore conditions that affect respiration.6 655 9. let sit for 5 minutes.3 530 23. Place temp sensor into beaker.8 2168 23. 2 3 4 5 6 7 8 9 10 C.3 2286 33.7 2286 23. x. Replace CO sensor in bottle.3 1223 10.3 997 10. Place open bottle into beaker with ice water.0 2607 34 24.8 1509 22.5 2506 24 1071 10.2 450 10.6 24. Data a.0 1611 32.2 336 9.0 24. ix.

In Part A. If the potato cores in Part C were allowed to dry out before use in this experiment. If the bags in Part B were placed in . which adds mass to the bag. the potential could be too varied for the experiment.4 M sucrose instead of distilled water. what molecules could diffuse through the dialysis tubing and which stayed inside? Why? y The glucose was able to diffuse through the tubing because of its smaller molecular structure and its lower density. In Part B. because it is at equilibrium with the solution. More water would be absorbed when you placed the potato core into the water solution. VIII. The . Conclusion . Why is measuring the temperature an important part of the calculation of osmotic potential? y Without the temperature.4 M bag would not change at all. while the bags above . Post-Lab Questions 1. why did the mass of the bags change? y The mass of the bags changed because of the bag¶s higher concentration. The solution naturally moved from the water in the cup to the bag due to osmosis.4 M would decrease in mass. because everything below . 2. how would the results be affected? y They would gain more water if they were allowed to dry out before use. The heat or cold would change the data. what would the results be? y The results would change. 3. 5. 4.4 M would increase in mass.VII.

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