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Cellular Respiration

AP Biology Lab #3

Janice Chan

Period 2

Partners: Kevin Ta,

3. pressure potential. including the transportation of ³vitally important nutrients and compounds without expenditure of excess metabolic energy. water. 2.´ The mechanisms are important to cells because they allow for many life functions of the cell.´ y The selectively permeable membrane has different cellular concentrations on either side. . so the osmosis moves the solution from lower concentration to higher concentration. What is the relationship between solute concentration. ³the absorption and early transport of water into the root system of plants. What are the effects of a selectively permeable membrane on diffusion and osmosis between two solutions separated by the membrane? y When you have a membrane that separates two solutions. ³small. What are the mechanisms of diffusion and osmosis and their importance to cells? y Diffusion is known as the movement of molecules from a higher concentration to a lower concentration. helps transport water in the xylem. What are the effects of solute size and concentration gradients on diffusion and osmosis between two solutions separated by the membrane? y The solute size does not affect the diffusion of the membrane. such as oxygen. the water moves from a region of low solute concentration to a region of high solute concentration. and glucose to pass freely. The higher the concentration.Janice Chan Dr. with different solute concentrations on either side of the membrane« In osmosis. Pre-lab Questions 1. 5. ³in water separated by a selectively permeable membrane. the faster the water will flow through the membrane. and. What is water potential? y A solute¶s relative concentration. Fuller AP Biology ± Period 2 30 September 2010 AP Biology Lab #3 ± Cellular Respiration I. carbon dioxide. Water always moves from an area of higher water potential to an area of lower water potential. it restricts certain materials from passing freely through the membrane.´ Osmosis and its impact on plant cells allows for. 4. neutrally charged molecules. the membrane allows certain. Osmosis is a special type of diffusion in which the action occurs. and the water potential of a solution? y Pressure potential and osmotic potential assist water potential in reaching equilibrium. but the concentration gradient does. With diffusion and osmosis. with transpirational pull.

and equilibrium has been reached. 6. and when the pressure potential is equal to the osmotic potential. What is the relationship of molarity to osmotic concentration? y As molarity increases. water potential is 0.Solute concentration equals water potential. so does osmotic concentration. y .

Press start button on Xplorer to begin data display. vi. Record again. ii. Place 25 dry peas into CO bottle. until temp reaches 10 C. Hypothesis a. be sure that jar is sealed by stopper on sensor. remove soaked peas [place on paper towel] and invert and shake open bottle. Rid all CO that may be present. every minute for 15 minutes. replace CO sensor. Cellular Respiration Objective a. II. Record concentration of CO every minute for 15 minutes. If germinating peas are cooled from room temperature to 10 degrees C. If peas are made to leave dormancy and germinate. To measure the conditions that affect the rate of cell respiration. III. then their respiration will decrease. iv. Ice V. Press start button twice. . then their respiration will increase. iii. Place 25 soaked peas into bottle. 500 ml beaker 5. Remove dry peas from bottle. invert open bottle and shake for several seconds. IV. 25 soaked [germinating] pea seeds 4. restart data collection.Cellular Respiration Lab I. Materials 1. b. vii. Fill 500 ml half beaker half full with water and add ice while stirring. v. Remove sensor. insert CO sensor into bottle. PASCO Xplorer GLX with CO sensor and temperature sensor 2. 25 dry pea pods 3. Procedure a. their cellular respiration will increase. Part A i. Title a. c. drain CO again. Place temp sensor next to bottle. If there is light coming from a heat lamp shining down on the peas.

9 833 10.3 2286 33.7 964 30 24.2 336 9.6 656 26 25 1280 22.9 24. Data a.7 25 732 22.9 903 10.5 1370 31.2 2923 24.9 565 10.0 3645 31.8 1711 22.8 . [Concentration & temp] Add ice as needed to keep temp as close to 10 C as possible.6 1020 10.6 24. ix. let sit for 5 minutes.2 1181 10.8 24. Place temp sensor into beaker. x. 2 3 4 5 6 7 8 9 10 C. Replace CO sensor in bottle.0 1870 33 24.4 24.6 25 1029 22.6 2048 22.8 3115 34 24.3 997 10. Red = Soaked.3 2604 24 1091 9.7 1900 22.5 2506 24 1071 10.6 484 10. create and carry out own experiment to explore conditions that affect respiration.0 24. Key i. regulating temperature at 10 Orange = With light.6 2870 33 24.6 655 9. Time [ Minutes] Concentration [PPM] Temp [ C] Concentration [PPM] Temp [ C] Concentration [PPM] Temp [ C] Concentration [PPM] Temp [ C] 11 12 13 14 15 407 543 543 544 543 546 541 542 545 546 551 552 544 543 540 545 24.7 2286 23. Blue = With ice. Return soaked peas to bottle.9 1147 30.8 2168 23. Place open bottle into beaker with ice water.1 24.4 24.2 2714 24 1142 9.4 791 10. VI.8 741 10. iii.2 450 10.0 1611 32. iv.3 1223 10.0 2607 34 24. Record again every minute for 15 minutes. Using germination and dry peas. press again twice to restart data collection.0 3372 33.7 507 23.0 2080 33. ii.2 805 28.8 417 22.8 1509 22.3 530 23.viii. 1 Black = Original.3 2818 24.8 2389 23.

If the bags in Part B were placed in . Conclusion . the potential could be too varied for the experiment. because everything below . how would the results be affected? y They would gain more water if they were allowed to dry out before use. The heat or cold would change the data.4 M would increase in mass. If the potato cores in Part C were allowed to dry out before use in this experiment. In Part B. 4. what would the results be? y The results would change. 3. In Part A. while the bags above . what molecules could diffuse through the dialysis tubing and which stayed inside? Why? y The glucose was able to diffuse through the tubing because of its smaller molecular structure and its lower density. More water would be absorbed when you placed the potato core into the water solution.4 M bag would not change at all.4 M sucrose instead of distilled water.4 M would decrease in mass. The solution naturally moved from the water in the cup to the bag due to osmosis. 5. which adds mass to the bag. The . Why is measuring the temperature an important part of the calculation of osmotic potential? y Without the temperature.VII. VIII. because it is at equilibrium with the solution. why did the mass of the bags change? y The mass of the bags changed because of the bag¶s higher concentration. 2. Post-Lab Questions 1.