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E PUBLICATION JOURNAL
>Premier Cibelli, J.B. The health profile 20 1 13. http://web.b.ebscohost.com/ehost/detail/detail?v
>EBSCOHOST Campbell, K.H. Jan2002 of cloned animals. Nature id=3&sid=b528d1b3-2ab5-4650-8414-
Seidel, G.E. Biotechnology 81a7f4cc407d%40pdc-v-
West, M.D. sessmgr06&bdata=JnNpdGU9ZWhvc3QtbGl2ZQ%3
Lanza, R.P. d%3d#AN=8782378&db=aph
>Premier Feb2013 Handmade Cloned PLoS ONE 8 2 1-9 http://web.b.ebscohost.com/ehost/detail/detail?v
>EBSCOHOST Zhang, P. Transgenic Sheep id=3&sid=9de9eb30-8ec2-407a-b7aa-
Liu, P. Rich in Omega-3 035db79f8175%40sessionmgr120&bdata=JnNpdG
Dou, H. Fatty Acids. U9ZWhvc3QtbGl2ZQ%3d%3d#AN=87624137&db=
Chen, L. aph
Chen, L.
Lin, L.
Tan, P.
Vajta, G.
Gao, J.
Du, Y.
Ma, R. Z.
>Premier Flint, J. Apr2005 Strategies for Nature Reviews 6 4 271-286 http://web.a.ebscohost.com/ehost/detail/detail?v
>EBSCOHOST Valdar, W. mapping and Genetics id=3&sid=f775dc47-5403-409e-b783-
Shifman, S. cloning ec11b62ef497%40sdc-v-
Mott, R. quantitative trait sessmgr05&bdata=JnNpdGU9ZWhvc3QtbGl2ZQ%3
genes in rodents. d%3d#AN=16621917&db=aph

>Premier Zwick, M.M. Dec2000 Genetic engineeri New Genetics & 19 3 269-281 http://web.a.ebscohost.com/ehost/detail/detail?v
>EBSCOHOST ng: risks and haza Society id=3&sid=ad756f17-ec0a-46dd-8311-
rds as perceived c326b0c804a1%40sessionmgr4006&bdata=JnNpd
by the German GU9ZWhvc3QtbGl2ZQ%3d%3d#AN=4139570&db=
public. aph
DOI RESEARCH/OBJECTIVES METHODOLOGY SOURCE OF DATA FINDINGS LIMITATION CONCLUSION RECOMMENDATION
Studies will
focus on identifying
the most suitable
technical
and biological
parameters for nuclear
transfer that will lead
to the reduction and
perhaps
the elimination of
losses observed during
the gestational and
neonatal period.
DOI RESEARCH/OBJECTIVES METHODOLOGY SOURCE OF FINDINGS LIMITATION CONCLUSION RECOMMENDATION
DATA
We aimed to establish In this study, we We successfully In the recombinant In summary, our
DOI:10.1371/journal.pone.0055941 a reliable and robust used the modified generated 3 clonal cells expressing study
HMC procedure to handmade cloning transgenic mfat-1, demonstrated
generate transgenic (HMC) established sheep that carry n26 fatty acids once again that
sheep with increased in cattle and pig to and express the (18:2n26, 20:4n26, and the alternative
levels of omega-3. produce transgenic nematode mfat- 22:4n26) were HMC is helpful
sheep with 1 gene. successfully converted to accelerate the
elevated levels of to the corresponding speed of
omega-3 (n23) n23 (18:3n23, transgenic
fatty acids. 20:5n23, and 22:5n23), animal research
and the ratio of with cost
n26/n23 were reduced efficiency and
from 8.60:1 to 0.45:1, a easy in
change of more than operation.
19-folds of reduction
compared to the non-
transfected cells (Figure
1D, Table 1). The
candidate clones
selected (F-1-1, F-6-5,
and H-6-6) showed the
normal karyotype (data
not shown). We
eventually selected the
clone H-6-6 for the
subsequent HMC
procedure; partially
due to
a relatively higher ratio
of n26/n23 conversion
was observed in
that clone (Table 1)
DOI RESEARCH/OBJECTIVES METHODOLOGY SOURCE OF DATA FINDINGS LIMITATION CONCLUSION RECOMMENDATION
10.1038/nrg1576 We discuss the We review the The National Center new resources, Throughout this review we
realized or potential potential of these for Biotechnology such as have emphasized two
success of new strategies to identify Information (NCBI) chromosome points: the importance of
approaches to identify genes that underlie lists 700 QTLs that are substitution being able to resolve QTLs
genes that underlie QTLs in rodents. found in rats (see the strains and the that contribute to less than
QTL’s. We focus on The experimental Entrez Gene web site proposed 5% of the phenotypic
methods to identify design is simple — it in the Online links Collaborative variance into small regions
genetic variants that requires offspring box), and the mouse Cross, together and the need for methods
have only a small from four crosses. An genome database with new that identify genes, not
effect on the inbred animal bearing currently contains analytical sequence variants, at the
phenotype (by which one QTL allele (for 2,050 mouse QTLs tools, including QTL. e subtle105. Given its
we mean that the example ‘high’) is (see the Mouse probabilistic importance for determining
variants account for mated to an inbred Genome Informatics ancestral the success of QTL cloning,
10% or less of the animal with a null web site in the Online haplotype it is perhaps surprising that
phenotypic variation allele of the gene of links box). reconstruction in so little attention has been
of a quantitative trait. interest (‘m’) and also outbred mice, paid to emerging clues that
to the co-isogenic Yin–Yang reveal considerable
wild-type animal crosses and in variation in the genetic
(‘wt’). silico analysis of architecture of phenotypes.
sequence It seems that the number of
variants in many loci, their relative effects,
inbred strains, and how they interact with
could make QTL each other and the
cloning tractable. environment, might vary
considerably between
phenotypes, indicating that
some phenotypes might be
more tractable to QTL
cloning than others
DOI RESEARCH/OBJECTIVES METHODOLOGY SOURCE OF DATA FINDINGS LIMITATION CONCLUSION RECOMMENDATION
to clarify the Most findings of the The theses are based qualitative data
semantic space of that qualitative study on 48 provided evidence
technology in public have been verified by 'Leitfadeninterviews', for the dual
opinion based on the survey data, the made between 1995 character of genetic
qualitative data latter, however, are and 1997 and engineering: people
and, second, to not presented in this complemented by a base their estimate
develop hypotheses paper. The representative of beneŽ ts and risks
and instruments to interpretations are survey carried out in on the associations
answer the question of based on 48 Germany in 1997, that they attribute to
how and why people qualitative interviews which contains 1501 the different Ž elds
judge genetic cases. of application. in the
engineering as they case of prenatal
do. In particular we human genetics and
discuss what concepts genfood, people
of risk are available, tend to
and to what extent the justify their
assumptions judgements on
of risks and hazards values based on
are used in ethics, emotions or
constructing value even system
judgements. criticism.