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Water pollution is one of the main problems in the Philippines. The lack

of wastewater treatment plants and poor regulation and assessment of

aquatic environments brought unwanted effects to many organisms in

different trophic levels. The rationale of this study is to assess the levels of

phytotoxicity in different aquatic environments in Hilongos Municipality using

Duckweeds (Lemna minor) Dose / Response Test and Lettuce (Lactuca

sativa L.) seed germination tests. Water Samples were collected from

Highway Canal, San Isidro Canal, Central Canal, Market Wastewater and

Salog River. Concentrations with three replicates were made at 100%, 60%,

36%, 22% , 13%. In duckweeds test, the researchers used frond number and

mortality as test endpoints while in lettuce seeds test, parameters used were

seed germination and root elongation. Results showed that Market

Wastewater possessed the highest phytotoxicity among all water samples in

both duckweeds and lettuce seeds test. At 22% concentration or higher, the

duckweeds exhibited discoloration and the presence of lesions and chlorosis

were observed. Similarly, in lettuce seeds test, Market Wastewater also

showed significant phytotoxicity in terms of seed germination and root

elongation at 95% Confidence level. Central Canal, Highway Canal, Salog

River and San Isidro showed significant phytotoxicity at 36% concentration or

higher. Loss of green pigments and inhibition of frond reproduction were also

detected. However In Lettuce seeds test, water sample from Central Canal

only exhibited significant level of phytotoxicity while other water samples

except the Market Wastewater were not statistically significant (p > 0.05).