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T-1 Water = 22,031.

44 kg To Recovery and Treatment Facility


K-1
Collected Chicken Bones Supernatant
Collected Chicken Bones Crushed Chicken Bones (Oversized) Steam at 150 Condensate at 150
Ca3(PO4)2 = 2,964.00 kg Unbound Water = 21,811.13 kg
Ca3(PO4)2 = 3,916.21 kg Ca3(PO4)2 = 955.17 kg 2,644.58 kg/day 2,644.58 kg/day K-3
Proteins = 1,653.00 kg Suspended Fats = 53.40 kg
Proteins = 2,184.04 kg Proteins = 532.69 kg Wet Bone Chips
C102H149O88 N31 = 1,487.70 kg TOTAL = 21,864.53 kg
C102H149O88 N31 = 1,965.64 kg C102H149O88 N31 = 479.42 kg Solid
Non-Collagenous F-1
Non-Collagenous Non-Collagenous Ca3(PO4)2 = 2,862.65 kg
Protein = 165.30 kg Bone Chips
Protein = 218.40 kg Protein = 53.27 kg Proteins = 1,596.48 kg
CaCO3 = 684.00 kg Ca3(PO4)2 = 2,864.08 kg HCl Solution
CaCO3 = 903.74 kg CaCO3 = 220.42 kg C102H149O88 N31 = 1,436.83 kg
Bound Moisture = 342.00 kg Proteins = 1,597.28 kg Hydrochloric Acid = 1,787.26 kg
Bound Moisture = 451.87 kg Bound Moisture = 110.21 kg Non-Collagenous
Fats = 57.00 kg C102H149O88 N31 = 1,437.55 kg Water = 42,894.29 kg
Fats = 75.31 kg Fats = 18.37 kg Protein = 159.65 kg
TOTAL = 5,700.00 kg Non-Collagenous TOTAL = 44,681.55 kg
TOTAL = 7,534.02 kg TOTAL = 1,836.87 kg CaCO3 = 660.61 kg
= -371,798.55 / Protein = 159.73 kg Bound Moisture = 330.31 kg
CaCO3 = 660.94 kg Fats = 1.65 kg
K-2 Bound Moisture = 330.47 kg Total = 5,451.70 kg
Mass Losses = 2.85 kg Mass Losses = 2.85 kg
Collected Chicken Bones Fats = 55.08 kg Liquid
Ca3(PO4)2 = 3,916.21 kg Added Water = 22,031.44 kg Unbound Water = 22,031.44 kg J-1
E-1 Proteins = 2,184.04 kg TOTAL = 27,539.29 kg Degreased Bones
Water Evaporated = 1,470.31 kg Gelatin Solution Suspended Fats = 53.40 kg
C102H149O88 N31 = 1,965.64 kg = +5,590,475.98 / Total = 22,084.84 kg Solid
Water = 3,191.68 kg Non-Collagenous Ca3(PO4)2 = 2,861.22 kg
Mass Losses = 2.29 kg TOTAL = 27,536.54 kg
C102H151O89 N31 = 1,369.19 kg Protein = 218.40 kg Proteins = 1,595.68 kg
Sodium Hydroxide Solution HCl = 2.19 kg Mass Losses = 2.75 kg C102H149O88 N31 = 1,436.11 kg
CaCO3 = 903.74 kg
NaOH = 2.45 kg Fats = 1.65 kg Bound Moisture = 451.87 kg Mass Losses = 2.73 kg Non-Collagenous
Dissolved CaCl2 = 0.15 kg Bone Chips (Desired)
Water = 42.06 kg Fats = 75.31 kg Ca3(PO4)2 = 2,864.08 kg Protein = 159.57 kg
TOTAL = 44.51 kg Dissolved Ca(H2PO4)2 = 0.12 kg TOTAL = 7,531.17 kg K-4 CaCO3 = 660.28 kg
TOTAL = 4,564.98 kg Proteins = 1,597.28 kg
Demineralized Bones Bound Moisture = 330.14 kg
= +3,126,569.52 / C102H149O88 N31 = 1,437.55 kg
Solid Fats = 1.65 kg
F-5 Mass Losses = 2.76 kg Non-Collagenous
Ca3(PO4)2 = 1,287.55 kg Total = 5,448.97 kg
Basic Gelatin Solution Protein = 159.73 kg
Proteins = 1,594.88 kg Liquid
Thick Liquor To Recovery and Treatment Facility CaCO3 = 660.94 kg
Steam at 150 Condensate at 150 C102H149O88 N31 = 1,435.39 kg Unbound Water = 220.31 kg
Water = 1,719.77 kg Crushed Chicken Bones (Undersized) Bound Moisture = 330.47 kg
1,479.54 kg/day 1,479.54 kg/day Non-Collagenous Total = 220.31 kg
C102H151O89 N31 = 1,368.51 kg Ca3(PO4)2 = 95.52 kg Fats = 55.08 kg
Protein = 159.97 kg HCl Solution
HCl = 2.19 kg Proteins = 53.27 kg TOTAL = 5,507.85 kg
To Recovery and Treatment Facility CaCO3 = 297.13 kg Hydrochloric Acid = 1,787.26 kg
Fats = 1.65 kg C102H149O88 N31 = 47.94 kg
Spent Ossein HCl Solution Bound Moisture = 329.96 kg Water = 42,894.29 kg
Dissolved CaCl2 = 0.15 kg Non-Collagenous
Ca3(PO4)2 = 1,285.63 kg Hydrochloric Acid = 210.57 kg Fats = 1.65 kg Total = 44,681.55 kg
Dissolved Ca(H2PO4)2 = 0.12 kg Protein = 5.33 kg
Bound Moisture = 329.48 kg Water = 842.26 kg Total = 3,511.17 kg TOTAL = 50,350.83 kg
Total = 3,092.38 kg CaCO3 = 22.04 kg
CaCO3 = 296.68 kg TOTAL = 1,052.83 kg Saturated Acid Solution = -1,796,728.98 /
Sodium Hydroxide Solution Bound Moisture = 11.02 kg
Non-Collagenous Protein = 159.25 kg Unbound Water = 43,179.97 kg
NaOH = 2.45 kg Fats = 1.84 kg
C102H149O88 N31 = 71.66 kg Excess HCl = 794.79 kg
Water = 42.06 kg TOTAL = 183.69 kg
TOTAL = 2,142.70 kg F-3 Dissolved CaCl2 = 1,516.27 kg
Total = 44.51 kg
Water = 3,186.56 kg Ossein Dissolved Ca(H2PO4)2 = 1,187.87 kg
TOTAL = 3,136.89 kg Cooler 10 Exit water at 25
Steam at 150 Condensate at 150 Solid Total = 46,678.90 kg
= -38,672.80 / Q = -1,796,728.98 kJ/day 28,614.89 kg/day
3.00 kg/day 3.00 kg/day Ca3(PO4)2 = 1,286.91 kg TOTAL = 50,190.07 kg
J-2 Proteins = 1,594.08 kg
Acid-Conditioned Ossein C102H149O88 N31 = 1,434.67 kg
Cooling water at 25 Exit water at 35 Solid Mass Losses = 1.75 kg Inlet water at 25
Non-Collagenous
923.86 kg/day 923.86 kg/day T-2 Ca3(PO4)2 = 1,286.27 kg 28,614.89 kg/day
Protein = 159.41 kg To Recovery and Treatment Facility
Inoculum Proteins = 1,593.28 kg CaCO3 = 296.98 kg Saturated Acid Solution
Nutrient Broth = 13.11 kg C102H149O88 N31 = 1,433.95 kg Bound Moisture = 329.80 kg Unbound Water = 42.748.17 kg To Recovery and Treatment Facility
Streptomyces omiyaensis = 50.67 kg Non-Collagenous Fats = 1.65 kg
Mass Losses = 0.68 kg Excess HCl = 786.84 kg Carbon Dioxide Gas
Protease = 27.36 kg Protein = 159.33 kg Total = 3,509.42 kg Dissolved CaCl2 = 1,501.11 kg Carbon Dioxide = 159.79 kg
TOTAL = 91.14 kg CaCO3 = 296.83 kg Saturated Acid Solution Dissolved Ca(H2PO4)2 = 1,175.99 kg TOTAL = 159.79 kg
J-3 = +6,312.10 / Bound Moisture = 329.64 kg Unbound Water = 431.80 kg TOTAL = 46,212.11 kg
Collagen Hydrolysate Solution Fats = 1.65 kg Excess HCl = 7.95 kg Mass Losses = 0.97 kg
Liquid K-5 Total = 3,507.67 kg Dissolved CaCl2 = 15.16 kg To Recovery and Treatment Facility
Water = 1,762.92 kg Collagen Hydrolysate Solution Liquid
C102H151O89N31 = 1,367.83 kg Dissolved Ca(H2PO4)2= 11.88 kg Saturated Acid Solution
Liquid Unbound Water = 12.74 kg LEGEND:
NaCl = 3.53 kg Total = 466.79 kg Unbound Water = 1,261.32 kg
Water = 1,754.42 kg Excess HCl = 2.19 kg E-1 VACUUM EVAPORATOR
NaOH = 0.02 kg HCl Solution Excess HCl = 216.33 kg
C102H153O90N31 = 1,361.01 kg Dissolved CaCl2 = 0.15 kg F-1 DEGREASING TANK
Fats = 1.65 kg Hydrochloric Acid = 210.57 kg Dissolved CaCl2 = 15.01 kg
C102H151O89N31 = 13.67 kg Dissolved Ca(H2PO4)2 = 0.12 kg J-1 DEMINERALIZER
Dissolved CaCl2 = 0.15 kg Water = 842.26 kg Dissolved Ca(H2PO4)2 = 11.76 kg
NaCl = 3.53 kg Total = 15.20 kg K-1 ROLL CRUSHER
Dissolved Ca(H2PO4)2 = 0.12 kg Total = 1,052.83 kg TOTAL = 1,504.42 kg
NaOH = 0.02 kg Added Water = 3,186.56 kg T-1 AIR BLAST FREEZER
Nutrient Broth = 13.11 kg TOTAL = 5,029.04 kg
Fats = 1.65 kg TOTAL = 6,709.43 kg F-2 DILUTION TANK
Total = 3,149.32 kg = -33,239.97 /
Dissolved CaCl2 = 0.15 kg = -9,433.96 / J-2 COLLAGEN HYDROLYZER
Solids Dissolved Ca(H2PO4)2 = 0.12 kg K-2 DOUBLE DECKED SCREENER
Streptomyces omiyaensis = 50.67 kg Nutrient Broth = 13.10 kg T-2 INOCULUM TANK
Protease = 27.36 kg Total = 3,147.66 kg Exit water at 25 Cooler 10 Inlet water at 25 F-3 ACIDIFICATION TANK
Total = 78.03 kg Exit water at 35 Cooling water at 25 529.38 kg/day Q = -33,239.97 kJ/day 529.38 kg/day J-3 GELATIN HYDROLYZER
Solids
TOTAL = 3,227.35 kg 225.37 kg/day 225.37 kg/day K-3 DECANTER CENTRIFUGE
Streptomyces omiyaensis = 50.64 kg
= +2,602,868.09 / Protease = 27.35 kg Mass Losses = 1.75 kg T-3 FREEZE DRYER
Total = 77.99 kg Mass Losses = 1.75 kg F-4 DILUTION TANK
TOTAL = 3,225.65 kg Water Evaporated = 1,644.60 kg J-4 MIXED BED ION EXCHANGE
J-4 COLUMN
Cooling water at 25 Exit water at 35 Filtered Collagen Hydrolysate Solution T-3 T-4 K-4 DECANTER CENTRIFUGE
62,180.32 kg/day 62,180.32 kg/day To Recovery and Treatment Facility Water = 1,666.70 kg Deionized Collagen Hydrolyzed Solution Collagen Hydrolysate Powder T-4 COMMERCIAL REFRIGERATOR
Retentate C102H153O90N31 = 1,361.01 kg Water = 1,669.64 kg Water = 25. 04 kg F-5 BASIC CONDITIONING TANK
Mass Losses = 1.70 kg Nutrient Broth = 13.10 kg NaCl = 3.53 kg C102H153O90N31 = 1,361.01 kg C102H153O90N31 = 1,361.01 kg K-5 MICROFILTER
Streptomyces omiyaensis = 50.64 kg NaOH = 0.02 kg Ions = 0.87 kg Impurities = 0.87 kg
Protease = 27.35 kg Dissolved CaCl2 = 0.15 kg TOTAL = 3,031.52 kg TOTAL = 1, 386.92 kg BASIS:
C102H151O89N31 = 13.67 kg Dissolved Ca(H2PO4)2 = 0.12 kg = +4,376,411.89 / = -10,018.71 / Annual Plant Capacity:
Fats = 1.65 kg TOTAL = 3,031.52 kg 431,137 kg Hydrolyzed Collagen/year
Water = 87.72 kg Daily Plant Capacity:
TOTAL = 194.13 kg 1,.440 kg Hydrolyzed Collagen/day
OVERALL MASS & ENERGY BALANCE
Working Days:
PRODUCTION OF HYDROLYZED COLLAGEN FROM CHICKEN BONES 300 days/year