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Curriculum Vitae

Daniel Elger Wagner, Ph.D.

Department of Systems Biology,
Harvard Medical School
200 Longwood Ave, Warren Alpert 536
Boston, MA 02115
Mobile: 802-309-9497

2014-Present K99/R00 and HHMI-LSRF Postdoctoral Fellow
Department of Systems Biology, Harvard Medical School, Boston, MA
Advisors: Dr. Sean Megason and Dr. Allon Klein
2012-2014 Post-Doctoral Associate
Whitehead Institute for Biomedical Research, Cambridge, MA
Advisor: Dr. Peter Reddien
2005-2012 Ph.D. in Biology
Massachusetts Institute of Technology, Cambridge, MA
Dissertation Title: “Cellular and Genetic Mechanisms of New Tissue Production in the
Regenerating Planarian Schmidtea mediterranea”.
Advisor: Dr. Peter Reddien
1999-2003 B. S. in Biology (High Honors, Biology), Minor in Chemistry
Haverford College, Haverford, PA
Senior Thesis: Self-Assembly of Designed Peptide-based Nanofilaments and Nanoropes
Thesis Advisor: Dr. Robert Fairman
Professional Experience
2003-2005 Research Technician, University of Pennsylvania, Philadelphia, PA
Studied structural biology of DNA-protein interactions and gene regulation under the direction of
Dr. Mitchell Lewis
2001 Research Technician, Jefferson Medical College, Philadelphia, PA
Laboratory of Dr. Miriam Wahl
Studied intracellular pH regulation in endothelial cells
Teaching Experience
2017-2018 Lecturer; Single-Cell Sequencing: experimental design, analysis, and practical applications
(CB399), Harvard Medical School
2016 Lecturer; Single-Cell Genomics Workshop, Harvard Stem Cell Institute (HSCI)
2015 Research Mentor; Undergraduate Summer Internship in Systems Biology, Harvard HMS
2008-2009 Research Mentor; Undergraduate Research Opportunities Program (UROP), MIT
2008 (Fall) Teaching Assistant; Development and Evolution (7.22), MIT
2006 (Fall) Teaching Assistant; Experimental Biology (7.02), MIT
2003 (Spring) Teaching Assistant; Cell Structure and Function (Bio200), Haverford College
Grants & Awards
2017 NIH-NIGMS K99/R00 Pathway to Independence (1K99GM121852)
2015 HHMI Postdoctoral Fellowship of the Life Sciences Research Foundation (LSRF)
2005 MIT Praecis Presidential Fellowship
2003 Haverford College Departmental Honors: High Honors, Biology
2003 Ariel G. Lowy Prize for Biology Research, Haverford College
2002 Howard Hughes Medical Institute Interdisciplinary Scholarship, Haverford College
Invited Talks
2017 10 European Zebrafish Meeting, Budapest, Hungary
Talk title: “Mapping Vertebrate Differentiation Hierarchies with High-Throughput Single-Cell Transcriptomics”
2017 International Society for Stem Cell Research 2017 Annual Meeting, Boston, MA
Talk title: “Mapping Vertebrate Differentiation Hierarchies with High-Throughput Single-Cell Transcriptomics”
ISSCR Abstract Merit Award

Curriculum Vitae
Daniel Elger Wagner, Ph.D.

Invited Talks (continued)
2013 Planarian Meeting, Stowers Institute for Medical Research, Kansas City, MO
Talk title: “Single cell analysis reveals heterogeneity of the neoblast population”
2011 Gordon Research Conference (Developmental Biology), Proctor Academy, Andover, NH
“Clonogenic neoblasts are pluripotent adult stem cells and the source of new tissue for planarian regeneration”
2008 Planarian Meeting, Chicago, Il
Talk title: “Identification of stem cell regulatory genes in planarians”

Publications (* indicates co-first authors)

1. Wagner DE, Klein AM. Reconciling lineage history with cell identity at single-cell resolution.
(under review, Nature Review Genetics)
2. Diaz-Cuadros M*, Wagner DE*, Budjan C, Hubaud A, Touboul J, Michaut A, Al Tanoury Z, Yoshioka-
Kobayashi K, Niino Y, Kageyama R, Miyawaki A and Pourquié O. In vitro characterization of the human
segmentation clock.
(under review, Nature).
3. Wagner DE, Weinreb C, Collins ZM, Briggs JA, Megason SG, Klein AM. Single-cell mapping of gene
expression landscapes and lineage in the zebrafish embryo. Science. 2018; 360(6392):981-987
4. Briggs JA, Weinreb C, Wagner DE, Megason S, Peshkin L, Kirschner MW, Klein AM. The dynamics of
gene expression in vertebrate embryogenesis at single-cell resolution. Science. 2018; 360(6392)
5. Raj B, Wagner DE, McKenna A, Pandey S, Klein AM, Shendure J, Gagnon JA, Schier AF. Simultaneous
single-cell profiling of lineages and cell types in the vertebrate brain. Nat Biotechnol. 2018; 36(5):442-450
6. Wagner DE, Klein AM. Genetic Screening Enters the Single-Cell Era. Nature Methods 2017; 14(3):237-
7. Wang IE*, Wagner DE*, Reddien PW. Clonal Analysis of Planarian Stem Cells by Subtotal Irradiation and
Single-Cell Transplantation. Methods Mol Biol. 2018;1774:479-495
8. Owen JH*, Wagner DE*, Petersen CP, Reddien PW. teashirt is required for anteroposterior polarity
regeneration in planarians. Development 2015; 146(6):1062-1072
9. van Wolfswinkel JC*, Wagner DE*, Reddien PW. Single-cell analysis reveals functionally distinct classes
within the planarian stem cell compartment. Cell Stem Cell 2014; 15:326-339
10. Witchley JN, Mayer M, Wagner DE, Owen JH, and Reddien PW. Muscle Cells Provide Instructions for
Planarian Regeneration. Cell Reports 2013; 4:633–641
11. Wagner DE, Ho JJ, Reddien PW. Genetic Regulators of a Pluripotent Adult Stem Cell System in
Planarians Identified by RNAi and Clonal Analysis. Cell Stem Cell 2012; 10:299-311
12. Wagner DE*, Wang IE*, Reddien PW. Clonogenic neoblasts are pluripotent adult stem cells that underlie
planarian regeneration. Science 2011; 332:811-6
13. Wagner DE, Phillips CL, Ali WM, Nybakken GE, Crawford ED, Schwab AD, Smith WF, Fairman R.
Towards the Development of Peptide Nanofilaments and Nanoropes as Smart Materials. PNAS 2005;

Poster Presentations
2018 International Society for Stem Cell Research (ISSCR) Annual Meeting, Melbourne, Australia
2017 Keystone Symposia: “Single Cell Omics”, Stockholm, Sweden
2011 Planarian Regeneration Research Meeting, Kyoto, Japan
2010 HHMI Scientific Meeting, Chevy Chase, MD
2010 Society for Developmental Biology (SDB) Annual Meeting, Albuquerque, NM
2009 International Society for Stem Cell Research (ISSCR) Annual Meeting, Barcelona, Spain
2008 Northeast Regional Meeting of the Society of Developmental Biology, Woods Hole, MA