You are on page 1of 24

Project Details BITCOL 16 (Tolfenpyrad 15 + Bifenthrin 7.

5 % SE)
Sponsor GSP Crop Science Pvt. Ltd., Gujarat
Study Director Abhijeet Khot, Ph.D.
Study Personnel Mr. Sandeep Jagtap, M.Sc.
Management Kedar Deobhankar, Ph.D.
Study Initiated date 03 October 2017
Study Completion date 03 November 2017
Report date 14 November 2017
Project Code Number RLS/TS/039/05/17/002133
Report Code Number RLS/TS/039/05/17/002133/4
Ross Lifescience Private Limited
Test Facility Plot No. 96, Sector No. 10, PCNTDA,
Bhosari, Pune - 411026, Maharashtra, India.
GSP Crop Science Pvt. Ltd.,
404, Lalita Complex, 352/3, Rasala Road,
Sponsor Name Address Nr. Jain Temple, Navrangpura,
Ahmedabad-380009, Gujarat, India.
T0 Untreated control
T1 Standard Dose
T2 Double Recommended Dose (T2)
Limit of Detection (LOD) 0.005
Limit of Quantification (LOQ) 0.01
Limit of Quantification (LOQ) x 5 0.05
Limit of Quantification (LOQ) x 10 0.10

1 Location I

2 Location II

3 Location III

4 Location IV
Formulation Type Flowable Concentrate for FS
seed treatment. (FS)
AI AI Type Persistence in
Azoxystrobin Fungicides
Thiophanate Methyl Fungicides
Thiamethoxam Insecticide 2
3
Sathiyanarayanan.S, Ph.D. 4
5
6

750 ml/ha
1500 ml/ha
1.0 mg/L
1.0 mg/kg
1.0 mg/kg
1.0 mg/kg

Received Date
GBPUAT, Pantnagar,
Uttaranchal
Dr. PDKV, Akola,
Maharashtra
KVK, Jhabua, RVSKVV,
Madhya Pradesh
RCA, MPUAT, Udaipur,
Rajasthan

0.0005 mg/kg LOD


0.01 mg/kg LOQ
1.0 mg/kg
Mamurdi, Dehu Road,
Haveli, Pune, Maharashtra

Paddy Make
Substrate 1.30 % Azoxystrobin yxz
Pod 0.22 % Thiophanate Methyl yxz
Haulm 25.9 % Thiamethoxam yxz
Seed
Oil
de-Oil Pod
Soil

Test Facility Page No. 23

Before 17 April 2017 Nil

GLP
17 April 2017 17 April 2020 GLP certified

12 February 2013 11 February 2015 ISO/IEC-17025-2005 accredited


12 February 2015 23 September 2015 Nil
24 September 2015 23 September 2017 ISO/IEC-17025-2005 accredited
24 September 2017 05 December 2017 Nil
NABL 06 December 2017 05 December 2019 ISO/IEC-17025-2005 accredited

31 October 2011 31 March 2014


DSIR- recognized “in-house’ labo

DSIR 01 April 2014 31 March 2017


DSIR- recognized “in-house’ labo
01 April 2017 26 June 2017 Nil
27 June 2017 31 March 2020 DSIR- recognized “in-house’ labo

25 June 2015 18 May 2017 Approved by CIB&RC for registra


CIB&RC 19 May 2017 18 May 2019 Approved by CIB&RC for registra

GI/019 For all dates Sponsored by

0.10
B.NO. PURITY WT
1234 99.7 10.3 MG UPLC-MS/MS Acetonitrile
1234 99.3 10.7 MG GC-MS Ethyl acetate
1234 99.4 10.6 MG GC-MS Ethyl acetate

GLP

GLP certified (Chemistry including 5 batch analyses, and ecotoxicology)-GLP/C-098/2017-Valid -16-April 2020

ISO/IEC-17025-2005 accredited (NABL) - Chemical (Certificate No. T-2446) and Biological (Certificate No. T-2447) valid till 11-February-2

ISO/IEC-17025-2005 accredited (NABL) - Chemical (Certificate No. T-2446) and Biological (Certificate No. T-2447) valid till 23-September

ISO/IEC-17025-2005 accredited (NABL) - Chemical and Biological (Certificate No. TC-6633) valid till 05-December-2019

DSIR- recognized “in-house’ laboratory (TU/IV-RD/3310/2011). Valid till 31-March-2013

DSIR- recognized “in-house’ laboratory (TU/IV-RD/3310/2014). Valid till 31-March-2017

DSIR- recognized “in-house’ laboratory (TU/IV-RD/3310/2017). Valid till 31-March-2020

Approved by CIB&RC for registration data generation- as per 356th RC Meeting held on 25th June 2015 (valid till May-2017)
Approved by CIB&RC for registration data generation- as per 375th RC Meeting held on 19th May 2017 (valid till April-2019)

Sponsored by Science and Technology Park promoted by NSTEDB- Govt of India and University of Pune (License no-Gl/019)
T-2447) valid till 11-February-2015

T-2447) valid till 23-September-2017

ecember-2019

nse no-Gl/019)
1 Instruments Make
2 UPLC-MSMS Waters

3 Column Waters

Ms Upper India Scientific


4 Rotary Mixer Company, Nagpur
5 Roto Spin Tarsons
6 Analytical Balance M/S Contech India
Glass wares: Volumetric flasks,
Pipettes, Measuring cylinders, Ms Borosil Glass and
7 Separatory funnels and other A Glasswares, Mumbai
Grade Borosil glass ware
Merck Millipore India Pvt Ltd,
8 Millipore water purification system Bangalore
Dispersive clean-up tube containing
9 MgSO4 and PSA. QuEChERS- Waters
Model 1 Reagent
Acquity- H Class with Xevo TQD Mass Detector Acetonitrile (HPLC & UHPLC
2
Acquity grade)

UPLC BEH C18 (1.7 µm, 2.1 x 50 mm) 3 Water

NA 4 Sodium sulphate
NA 5 Magnesium sulphate
CA234 6 Florisil

NA

FIEA68222C

NA
Make
J.T. Baker

Millipore pure water system (S/N no:


FIEA68222C)

Merck
Fisher Scientific
?
Paddy Azoxystrobin

G4 Pod R1 R2 R3 Mean SD RSD % Recovery


LOQ 0.01 0.0090 0.0092 0.0087 0.0090 0.000 2.81 89.67
LOQ*5 0.05 0.0466 0.0449 0.0471 0.0462 0.001 2.50 92.40
LOQ*10 0.10 0.0891 0.0920 0.0984 0.0932 0.005 5.11 93.17
SS_LOQ*10 0.10 0.0888 0.0901 0.0962 0.0917 0.004 4.31 91.70
86.67
Paddy Azoxystrobin

G5 Haulm R1 R2 R3 Mean SD RSD % Recovery


LOQ 0.01 0.0088 0.0081 0.0091 0.0087 0.001 5.92 86.67
LOQ*5 0.05 0.0451 0.0464 0.0477 0.0464 0.001 2.80 92.80
LOQ*10 0.10 0.0926 0.0939 0.0948 0.0938 0.001 1.18 93.77
SS_LOQ*10 0.10 0.0915 0.0919 0.0921 0.0918 0.000 0.33 91.83

Paddy Azoxystrobin

G6 Seed R1 R2 R3 Mean SD RSD % Recovery


LOQ 0.01 0.0087 0.0090 0.0091 0.0089 0.000 2.33 89.33
LOQ*5 0.05 0.0431 0.0469 0.0487 0.0462 0.003 6.18 92.47
LOQ*10 0.10 0.0912 0.0929 0.0968 0.0936 0.003 3.07 93.63
SS_LOQ*10 0.10 0.0885 0.0911 0.0951 0.0916 0.003 3.63 91.57

Paddy Azoxystrobin

G7 Oil R1 R2 R3 Mean SD RSD % Recovery


LOQ 0.01 0.0087 0.0090 0.0091 0.0089 0.000 2.33 89.33
LOQ*5 0.05 0.0431 0.0469 0.0487 0.0462 0.003 6.18 92.47
LOQ*10 0.10 0.0912 0.0929 0.0968 0.0936 0.003 3.07 93.63
SS_LOQ*10 0.10 0.0885 0.0911 0.0951 0.0916 0.003 3.63 91.57

Paddy Azoxystrobin

G8 de-Oil R1 R2 R3 Mean SD RSD % Recovery


LOQ 0.01 0.0087 0.0090 0.0091 0.0089 0.000 2.33 89.33
LOQ*5 0.05 0.0431 0.0469 0.0487 0.0462 0.003 6.18 92.47
LOQ*10 0.10 0.0912 0.0929 0.0968 0.0936 0.003 3.07 93.63
SS_LOQ*10 0.10 0.0885 0.0911 0.0951 0.0916 0.003 3.63 91.57

Paddy Azoxystrobin

G9 Soil R1 R2 R3 Mean SD RSD % Recovery


LOQ 0.01 0.0087 0.0090 0.0091 0.0089 0.000 2.33 89.33
LOQ*5 0.05 0.0431 0.0469 0.0487 0.0462 0.003 6.18 92.47
LOQ*10 0.10 0.0912 0.0929 0.0968 0.0936 0.003 3.07 93.63
SS_LOQ*10 0.10 0.0885 0.0911 0.0951 0.0916 0.003 3.63 91.57
1 Pod Pod 4
2 Haulm Haulm
3 Seed Seed
4 Oil Oil
5 de-Oil de-Oil
6 Soil Soil
Paddy Thiophanate Methyl

% Degradation Pod R1 R2 R3 Mean SD RSD


LOQ 0.01 0.0090 0.0092 0.0087 0.0090 0.000 2.81
LOQ*5 0.05 0.0466 0.0449 0.0471 0.0462 0.001 2.50
LOQ*10 0.10 0.0891 0.0920 0.0984 0.0932 0.005 5.11
1.57 SS_LOQ*10 0.10 0.0888 0.0901 0.0962 0.0917 0.004 4.31

Paddy Thiophanate Methyl

% Degradation Haulm R1 R2 R3 Mean SD RSD


LOQ 0.01 0.0088 0.0081 0.0091 0.0087 0.001 5.92
LOQ*5 0.05 0.0451 0.0464 0.0477 0.0464 0.001 2.80
LOQ*10 0.10 0.0926 0.0939 0.0948 0.0938 0.001 1.18
2.06 SS_LOQ*10 0.10 0.0915 0.0919 0.0921 0.0918 0.000 0.33

Paddy Thiophanate Methyl

% Degradation Seed R1 R2 R3 Mean SD RSD


LOQ 0.01 0.0087 0.0090 0.0091 0.0089 0.000 2.33
LOQ*5 0.05 0.0431 0.0469 0.0487 0.0462 0.003 6.18
LOQ*10 0.10 0.0912 0.0929 0.0968 0.0936 0.003 3.07
2.21 SS_LOQ*10 0.10 0.0885 0.0911 0.0951 0.0916 0.003 3.63

Paddy Thiophanate Methyl

% Degradation Oil R1 R2 R3 Mean SD RSD


LOQ 0.01 0.0087 0.0090 0.0091 0.0089 0.000 2.33
LOQ*5 0.05 0.0431 0.0469 0.0487 0.0462 0.003 6.18
LOQ*10 0.10 0.0912 0.0929 0.0968 0.0936 0.003 3.07
2.21 SS_LOQ*10 0.10 0.0885 0.0911 0.0951 0.0916 0.003 3.63

Paddy Thiophanate Methyl

% Degradation de-Oil R1 R2 R3 Mean SD RSD


LOQ 0.01 0.0087 0.0090 0.0091 0.0089 0.000 2.33
LOQ*5 0.05 0.0431 0.0469 0.0487 0.0462 0.003 6.18
LOQ*10 0.10 0.0912 0.0929 0.0968 0.0936 0.003 3.07
2.21 SS_LOQ*10 0.10 0.0885 0.0911 0.0951 0.0916 0.003 3.63

Paddy Thiophanate Methyl

% Degradation Soil R1 R2 R3 Mean SD RSD


LOQ 0.01 0.0087 0.0090 0.0091 0.0089 0.000 2.33
LOQ*5 0.05 0.0431 0.0469 0.0487 0.0462 0.003 6.18
LOQ*10 0.10 0.0912 0.0929 0.0968 0.0936 0.003 3.07
2.21 SS_LOQ*10 0.10 0.0885 0.0911 0.0951 0.0916 0.003 3.63
Oil

ate Methyl Paddy Thiamethoxam

% Recovery % Degradation Pod R1 R2 R3 Mean SD


89.67 LOQ 0.01 0.0090 0.0092 0.0087 0.0090 0.000
92.40 LOQ*5 0.05 0.0466 0.0449 0.0471 0.0462 0.001
93.17 LOQ*10 0.10 0.0891 0.0920 0.0984 0.0932 0.005
91.70 1.57 SS_LOQ*10 0.10 0.0888 0.0901 0.0962 0.0917 0.004
86.67
ate Methyl Paddy Thiamethoxam

% Recovery % Degradation Haulm R1 R2 R3 Mean SD


86.67 LOQ 0.01 0.0088 0.0081 0.0091 0.0087 0.001
92.80 LOQ*5 0.05 0.0451 0.0464 0.0477 0.0464 0.001
93.77 LOQ*10 0.10 0.0926 0.0939 0.0948 0.0938 0.001
91.83 2.06 SS_LOQ*10 0.10 0.0915 0.0919 0.0921 0.0918 0.000

ate Methyl Paddy Thiamethoxam

% Recovery % Degradation Seed R1 R2 R3 Mean SD


89.33 LOQ 0.01 0.0087 0.0090 0.0091 0.0089 0.000
92.47 LOQ*5 0.05 0.0431 0.0469 0.0487 0.0462 0.003
93.63 LOQ*10 0.10 0.0912 0.0929 0.0968 0.0936 0.003
91.57 2.21 SS_LOQ*10 0.10 0.0885 0.0911 0.0951 0.0916 0.003

ate Methyl Paddy Thiamethoxam

% Recovery % Degradation Oil R1 R2 R3 Mean SD


89.33 LOQ 0.01 0.0087 0.0090 0.0091 0.0089 0.000
92.47 LOQ*5 0.05 0.0431 0.0469 0.0487 0.0462 0.003
93.63 LOQ*10 0.10 0.0912 0.0929 0.0968 0.0936 0.003
91.57 2.21 SS_LOQ*10 0.10 0.0885 0.0911 0.0951 0.0916 0.003

ate Methyl Paddy Thiamethoxam

% Recovery % Degradation de-Oil R1 R2 R3 Mean SD


89.33 LOQ 0.01 0.0087 0.0090 0.0091 0.0089 0.000
92.47 LOQ*5 0.05 0.0431 0.0469 0.0487 0.0462 0.003
93.63 LOQ*10 0.10 0.0912 0.0929 0.0968 0.0936 0.003
91.57 2.21 SS_LOQ*10 0.10 0.0885 0.0911 0.0951 0.0916 0.003

ate Methyl Paddy Thiamethoxam

% Recovery % Degradation Soil R1 R2 R3 Mean SD


89.33 LOQ 0.01 0.0087 0.0090 0.0091 0.0089 0.000
92.47 LOQ*5 0.05 0.0431 0.0469 0.0487 0.0462 0.003
93.63 LOQ*10 0.10 0.0912 0.0929 0.0968 0.0936 0.003
91.57 2.21 SS_LOQ*10 0.10 0.0885 0.0911 0.0951 0.0916 0.003
Thiamethoxam

RSD % Recovery % Degradation


2.81 89.67
2.50 92.40
5.11 93.17
4.31 91.70 1.57
86.67
Thiamethoxam

RSD % Recovery % Degradation


5.92 86.67
2.80 92.80
1.18 93.77
0.33 91.83 2.06

Thiamethoxam

RSD % Recovery % Degradation


2.33 89.33
6.18 92.47
3.07 93.63
3.63 91.57 2.21

Thiamethoxam

RSD % Recovery % Degradation


2.33 89.33
6.18 92.47
3.07 93.63
3.63 91.57 2.21

Thiamethoxam

RSD % Recovery % Degradation


2.33 89.33
6.18 92.47
3.07 93.63
3.63 91.57 2.21

Thiamethoxam

RSD % Recovery % Degradation


2.33 89.33
6.18 92.47
3.07 93.63
3.63 91.57 2.21
Location I GBPUAT, Pantnagar, Uttaranchal
GENERAL INFORMATION
Common Name UPFI 116

UPL Limited, UPL house, 610 B/2, Bandra


Name & address of the study sponsor Village, Bandra (E), Mumbai-400051,
Maharashtra, India

Type of Formulation Flowable Concentrate for seed treatment (FS)


Nature of chemical Insecticide and Fungicide
Test Substrate Soybean Pod, Haulm and Soil
Location CRC, GBPUAT, Pantnagar, Uttaranchal
Variety JS-335
APPLICATION DATA
Date of Sowing/ Transplanting 8/19/2016

Description of the plot plan/crop Randomized Block Design (RBD)


layout/cropping system

Plot Size 25 sq. m (5 m × 5 m)


Spacing 45 x 45 cm
Number of plants per plot/unit area 1110

Number of replications per Three


treatment
Method of application and
equipment Seed treatment

Growth stage of crop at time of Seed


application

Water volume As per requirement


No. of application One
Application date 8/19/2016
APPLICATION DETAILS
T2- 100 ml of UPFI 116/10 kg of seeds
Dose rate T8- Untreated Control
T9- 200 ml of UPFI 116/10 kg of seeds
SAMPLING DATA
No. of samples taken per 3
test/treatment

Sample quantity Soybean Pod, Haulm: 500 g and Soil: 200 g


Date of Sampling At harvest: 20/11/2016
Interval between last application and 93 days
sampling
Storage condition Under dry ice condition
Location II Dr. PDKV, Akola, Maharashtra
GENERAL INFORMATION
Common Name UPFI 116

UPL Limited, UPL house, 610 B/2,


Name & address of the study Bandra Village, Bandra (E), Mumbai-
sponsor 400051, Maharashtra, India

Flowable Concentrate for seed


Type of Formulation treatment (FS)
Nature of chemical Insecticide and Fungicide
Test Substrate Soybean Pod, Haulm and Soil
Location Dr. PDKV, Akola, Maharashtra
Variety JS-335
APPLICATION DATA
Date of Sowing/ Transplanting 8/20/2016

Description of the plot plan/crop Randomized Block Design (RBD)


layout/cropping system

Plot Size 25 sq. m (5 m × 5 m)


Spacing 30 x 10 cm
Number of plants per plot/unit 870 average
area
Number of replications per Three
treatment

Method of application Seed treatment

Growth stage of crop at time of Seed


application
As per requirement to make uniform
Water volume seed coating
No. of application One
Application date 8/20/2016
APPLICATION DETAILS
T2- 100 ml of UPFI 116/10 kg of seeds
Dose rate T8- Untreated Control
T9- 200 ml of UPFI 116/10 kg of seeds
SAMPLING DATA
No. of samples taken per 3
test/treatment
Soybean Pod, Haulm: 500 g and Soil:
Sample quantity 200 g
Date of Sampling At harvest: 26/11/2016
Interval between last application 98 days
and sampling
Storage condition Under dry ice condition
Location III KVK, Jhabua, RVSKVV, Madhya
Pradesh
GENERAL INFORMATION
Common Name UPFI 116

UPL Limited, UPL house, 610 B/2,


Name & address of the study Bandra Village, Bandra (E), Mumbai-
sponsor 400051, Maharashtra, India

Flowable Concentrate for seed


Type of Formulation treatment (FS)
Nature of chemical Insecticide and Fungicide
Test Substrate Soybean Pod, Haulm and Soil
KVK, Jhabua, RVSKVV, Madhya
Location Pradesh
Variety JS-335
APPLICATION DATA
Date of Sowing/ Transplanting 8/19/2016

Description of the plot plan/crop Randomized Block Design (RBD)


layout/cropping system

Plot Size 20 sq. m (4 m × 5 m)


Spacing 40 x 5 cm
Number of plants per plot/unit 1000 average
area
Number of replications per Three
treatment

Method of application Seed treatment

Growth stage of crop at time of Seed


application
As per requirement to make uniform
Water volume seed coating
No. of application One
Application date 8/19/2016
APPLICATION DETAILS
T2- 100 ml of UPFI 116/10 kg of
Dose rate seeds
T8- Untreated Control
T9- 200 ml of UPFI 116/10 kg of
seeds
SAMPLING DATA
No. of samples taken per 3
test/treatment
Soybean Pod, Haulm: 500 g and Soil:
Sample quantity 200 g
Date of Sampling At harvest: 30/11/2016
Interval between last application 103 days
and sampling
Storage condition Under dry ice condition
Location IV RCA, MPUAT, Udaipur,
Rajasthan
GENERAL INFORMATION
Common Name UPFI 116
UPL Limited, UPL house, 610
B/2, Bandra Village, Bandra (E),
Name & address of the study sponsor Mumbai-400051, Maharashtra,
India
Flowable Concentrate for seed
Type of Formulation treatment (FS)
Nature of chemical Insecticide and Fungicide
Test Substrate Soybean Pod, Haulm and Soil
RCA, MPUAT, Udaipur,
Location Rajasthan
Variety JS-335
APPLICATION DATA
Date of Sowing/ Transplanting 9/12/2016

Description of the plot plan/crop Randomized Block Design


layout/cropping system (RBD)

Plot Size 25 sq. m (5 m × 5 m)


Spacing 45 x 10 cm
Number of plants per plot/unit area 550 average

Number of replications per treatment Three

Method of application Seed treatment

Growth stage of crop at time of Seed


application
As per requirement to make
Water volume uniform seed coating
No. of application One
Application date 9/12/2016
APPLICATION DETAILS
T2- 100 ml of UPFI 116/10 kg
of seeds
Dose rate T8- Untreated Control
T9- 200 ml of UPFI 116/10 kg
of seeds
SAMPLING DATA
No. of samples taken per 3
test/treatment
Soybean Pod, Haulm: 500 g and
Sample quantity Soil: 200 g
Date of Sampling At harvest: 23/12/2016
Interval between last application and 103 days
sampling
Storage condition -20 ± 2⁰C