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4 Sodium chloride 5
5 Agar 15
dissolve medium completely .Media was sterilized completely by autoclaving at 121 for minutes.
Appendix 2: Composition of MacConkey Agar
1 Lactose monohydrate 10
3 Sodium chloride 5
7 Agar 13.5
50 g of the media was mixed in 1000ml distilled water .Heat with frequent agitation and boil for
one minute to completely dissolve the medium. Autoclave at 121 for 15 minutes.
Appendix 03 Composition of Manitol salt agar (MSA)
S. No Ingredients Quantity(gm/l)
3 D-Manitol 10.0
6 Agar 15.0
Suspend 111.025gm of MSA in 1000ml water and autoclave it at 121°C for 15 mints.
Appendix 04 Composition of phosphate buffer saline
1 Sodium chloride 8
1 Nacl 1
3 Tryptone 1