Staphylococcus Spp. Gram positive.

Clustered

Streptococcus Spp. Gram Positive

Chains

Streptococcus Gram Positive.

Pneumoniae Diplococcus
(Paired)
Moraxella Spp. Gram Negative
Neisseria Spp. Diplococcus

Neisseria Gonorrhoeae Gram Negative

Diplococcus

Spirillum Spp. Gram Negative

Spirochete
Flagellated
Bacillus Subtillis Gram Positive
Highly Flagellated
Transparent
Capsule
Endospore
(Green) in middle

Clostridium Tetani Gram Positive

Bacillus Body
Terminal Spore
Flagellar Motility

Enterobacteriaceae Gram Negative

Spp. Coccobacilli
Vibro Cholerae Gram Negative
Comma Shaped
(Vibrio)

Candida Albicans Gram Positive

(fungus) Budding/Oval
Yeast
Klebsiella Pneumoniae Gram Negative
Negative Staining Encapsulated
Bacillicus

Mycobacterium Spp. ZN – Ziehl-Neesan

(Red) in Acid Fast Stain
Staphylococcus Aureus Nutrient Agar
Smooth Colony Yellow- Gold Color
Smooth and Shiny
Solitary with Flat Edges

Bacillus Subtillis Nutrient Agar

Rough Colony White Large Colony
Unite When Adjacent
Jagged Edges
Dull Surface

Klebsiella Pneumoniae MacConkey Agar

Mucoid Colony Slimy Shiny Surface
United when Adjacent
Flat edges
Proteus Spp. Nutrient Agar
Spreading Colony Spreads
No Apparent Solitary
Colony

Staphylococcus Citreus Nutrient Agar

Pigmented Colony Lemon Yellow Colony

Staphylococcus Albus Blood Agar

(S. Epidermidis) White Color Colony
Pigmented Colony
Chromobacterium Nutient Agar
Violaceum Deep Purple Colony
Pigmented Colony

Serratia Marcescens Nutrient Agar

Pigmented Colony Red/Orange Pigment
(Prodigiosin)
Psuedomonas Nutrient Agar
Aeruginosa Vibrant Green Colony
Pigmented Colony Growth Penetrates
Medium

Staphylococcus Spp. Selective Media used to

Mannitol Salt Agar Differentiate Staph.
Species.
S. Aureus = Yellow
Other Staphs = White

Streptococcus α- Partial Lysis of blood

hemolytic cells leaving a
Alpha Hemolysis yellow/green/brown
discoloration (bruise-
like) around the colony
 Streptococcus β- Complete Lysis of blood
hemolytic cells. Media displays
Beta Hemolysis halos around the
bacterial colonies.

Streptococcus γ- No change in the blood

Hemolytic cells and no change in
Gamma Hemolysis the color of the medium

Reaction of IMVIC (E.coli and Klebsiella sp) – Indol, Methyl Red, Voges-Prosekauer , in Citrate
Figure 1 E. Coli

Figure 2 Klebsiella Spp.

Control with purple sugar indicator BCP (Bromocresol Purple)
TSiA - Triple Sugar Iron Agar (E. Coli and Klebsiella spp.)
Biochemical reactions