HW 1100pm SOW A0402 Mwa

Agilent 1100 Series
Liquid Chromatograph
Scope of Work – Preventive Maintenance
SCOPE OF WORK
Preventive Maintenance for the Agilent 1100 Series Liquid Chromatograph
1. SCOPE
This document provides details of the procedures necessary for the successful completion of a Preventive
Maintenance (PM) service call for the 1100 Series Liquid Chromatograph. It clarifies responsibilities
and assures uniform delivery of the service. Only Support Providers trained and certified by Agilent
Technologies will provide the service. Support Providers are Agilent Customer Engineers (CE’s)
and/or Agilent Authorized Support Providers (ASPs). References to other documents are included to
supply additional information (such as part numbers) or to provide clarity.
Delivery time for completion of the PM call is approximately 4.0 hours for a system comprising a pump,
degasser, column compartment, autosampler or manual injector, and a detector. One hour should be
added for each additional detector. Time starts when the Support Provider arrives at the Customer site
and ends when he/she leaves the site. The PM Checklist (a separate document) details the steps
necessary for completion.
NOTE: This document is to be used by Agilent Employees and Agilent ASPs, and is not to be
provided to Customers.
2. CUSTOMER RESPONSIBILITIES
In preparation for the service call, the Call Initiator (see section 3.1 below for a definition of the Call
Initiator) verifies with the Customer that the Support Provider has:
• Access to the liquid chromatograph scheduled for PM and adequate downtime to complete the
procedure.
• Adequate working space around the liquid chromatograph, with all supplies nearby.
• A representative (for example, the operator) present at all times when Support Provider is
performing this service.
Issued July, 2006 - Revision A.04.02 Agilent Restricted WAD Product Support Page 1 of 30
Agilent 1100 Series
• The system flushed with HPLC grade water, samples removed from the Autosampler (ALS), and
analytical columns removed from the column compartment.
• Normal operating supplies available including consumables and usage-dependent items such as
paper, pens, and solvents (HPLC grade water, isopropanol, methanol, acetone).
• Failure to provide the above may reduce applicable tests performed in the PM (that is, pressure test,
test injection).
3. AGILENT RESPONSIBILITIES
3.1 Scheduling—The Call Initiator
The service call begins with a call to the Customer by the “Call Initiator.” The Call Initiator may be the
Support Provider, a workload administrator, or a dispatcher, depending on local processes. The Call
Initiator:
1. Calls the Customer, schedules the date and time for service, and confirms Customer preparation.
2. Discusses possible problem areas before the Support Provider goes on site.
3. Asks the Customer to log a service call, if the instrument is not functional.
3.2 Support Provider Responsibilities

All Support Providers shall be Agilent trained and certified to work on 1100 Series Liquid
Chromatographs and the applicable detectors. Support Providers shall take necessary Electro-Static
Discharge (ESD) precautions (see appropriate Hardware Manual) when working on or near printed
circuit boards or other sensitive assemblies.
3.2.1 Delivery
1. Brings all necessary tools, supplies and parts to complete the procedure (see 1100 Series
Service/Reference Manuals for list of tools, supplies and parts) including:
− Restriction capillary (5022-2159) for seal wear-in procedure
− Blank nut (01080-83202) for pressure/leak test
2. Has access to the latest 1100 Firmware @ http://www.chem.agilent.com/scripts/cag_firmware.asp
Agilent 1100 Series
3. Supplies consumables used during the PM. See sections 4.1.4 through 4.1.11 and 4.1.16 of this
document for a list of included consumables.
4. Completes all steps in the PM Checklist applicable to the instrument.
NOTE: The PM Checklist is a separate document.
3.2.2 Documentation
1. Writes the date service was completed on the PM sticker.
2. Applies PM sticker to the instrument or instrument logbook.
3. Completes the Firmware Update/Installation Record Form if 1100 or 1200 firmware was updated.
4. Asks the Customer to sign the PM Checklist acknowledging the completion of service.
5. Signs the PM Checklist.
6. Affixes all data generated to the PM Checklist and gives it to the Customer for addition to the
instrument logbook.
4. PM CHECKLIST
Refer to the 1100 Series PM Checklist.
Support Provider completes the Checklist whether or not the instrument is fully operational.
4.1 PM Task Details

4.1.1 Customer responsibilities
1. Customer has system flushed with HPLC grade water, all samples removed from the ALS, and
analytical columns removed from the column compartment.
2. Customer has isopropanol (IPA) and HPLC grade water in the appropriate mobile phase containers
available for the procedure.
3. Customer saves all methods and sequence files prior to PM start.
Agilent 1100 Series
4.1.2 PM checklist
1. Verify that the Customer has removed analytical column. If still present, remove it now.
2. Verify with the Customer that system was flushed with HPLC grade water. If not, flush system
now.
3. If 1100 firmware is to be updated, verify that Customer has backed-up all methods.
4. Complete the PM Checklist in the order given.
5. If any module fails a test, record the results in the Test Results box and complete the rest of the
PM Checklist.
NOTE: In the event of any test failure, the Support Provider will log a service call, if the
Customer has a service contract. If the Customer does not have a service contract, the
Support Provider will inform the Customer of the failure (leak, inoperable sensor, test,
etc.), and offer to log a Time and Material service call for the Customer.
4.1.3 Liquid chromatograph system
1. Check firmware revisions of modules to be serviced:
− Follow the instructions provided by the Firmware Update/Installation Record Form for
1100/1200 Series HPLC Instruments available from the EPI Warehouse or Compliance CD-
ROM to check the firmware revisions currently installed.
− Review the compatibility text within the firmware update package to ensure the impact of
performing the update is fully understood by you and the customer prior to updating.
− If firmware is not current, ask Customer if they would like the firmware updated. If the
Customer chooses to have the firmware updated, confirm with the customer that they require
this form for their records and follow the procedure in the Firmware Update/Installation Record
Form . This form is to be used only once.
− If the firmware is updated again at a later date, an additional form should be used after obtaining
the required Customer approval.
Agilent 1100 Series
2. Record the module model number and serial number on the PM Checklist. If the model/serial
number is not included in the 1100 system, record N/A (not applicable) in this area and N/A in the
module Tests Result box.
3. Check the instrument logbook for recently serviced items. Exclude maintenance on items that were
replaced within 90 days of the current PM.
4. Perform a general inspection of each LC module. Alert the Customer to any potential safety issues
(waste container full, frayed wiring, drain tubes blocked, etc.).
5. Check that all waste trays/interfaces are properly mounted as per the 1100 Series Installation Guide.
6. Check for proper mounting and functionality of all leak sensors. Wash off each sensor with water.
If the sensor is functioning, the LC module indicator light will change to red indicating a leak. Dry
off sensor using a tissue or paper towel.
7. For tests performed, attach printed results to the PM Checklist.
Agilent 1100 Series
4.1.4 G1310A/11A/12A Isocratic / Quaternary / Binary Pumps
1. This maintenance procedure requires the consumables found in the parts list at the end of this
document.
2. Switch off power to the pump.
3. Remove and disassemble the pump head following the procedure in the 1100 Pump
Service/Reference Manual.
4. Clean the plungers with methanol or toothpaste. If toothpaste is used, take care to wash off any
residual toothpaste with water or methanol.
5. Clean the support rings and springs in the pump housing with water or methanol. A toothbrush can
be used to help remove stubborn deposits.
6. Exchange the pump seals following the procedure in the 1100 Pump Service/Reference Manual.
7. If the seal wash option is installed remove and replace wash seal and gasket as specified in
1100 Pump Service/Reference Manual.
8. Reassemble the pump head assembly following the procedure in the 1100 Pump Service/Reference
Manual.
9. Prepare a bottle of isopropanol and perform the seal wear-in procedure as defined in the 1100 Pump
Service/Reference Manual. The use of a restriction capillary (5022-2159) is required.
NOTE: This procedure is required for standard seals only (5063-6589). Performing this
procedure on the normal phase application seals (0905-1420) will damage them.
10. Replace the purge valve frit, gold seal, and plastic cap following the procedure in the 1100 Pump
11. Replace the outlet ball valve sieve (binary pump), gold seal, and plastic cap as described in the 1100
Pump Service/Reference Manual.
12. Using the same bottle of isopropanol, immerse solvent filter into the solvent and attach restriction
capillary to purge valve.
Agilent 1100 Series
13. Prime appropriate channel at 3 mL/min (lower flow rate may be needed to avoid overpressure) for
10 minutes with the purge closed. For a binary pump, purge Channel A. For a binary pump with a
solvent selection valve, purge Channel A2. For a quaternary pump, purge Channel D. This will
require >30 mL of isopropanol.
14. While purging, begin maintenance on the Sampler or Manual Injector and Column Compartment, if
installed.
15. Close purge valve.
16. Cap the outlet of the pump with a blanking nut (01080-83202). Perform the built-in leak test
following the procedure in the 1100 Pump Service/Reference Manual.
17. If the test fails, reprime the system for an additional 5 minutes and rerun the test. Continued failure
may indicate an improperly assembled pump or a loose fitting.
Agilent 1100 Series
4.1.5 G1361A Preparative Pumps
document.
3. Remove and disassemble the pump head following the procedure in the 1100 Prep Pump
5. Exchange the pump seals (front and rear, left and right) following the procedure in the 1100 Prep
6. Reassemble the pump head assembly following the procedure in the 1100 Prep Pump
7. Replace the filter cup in the multi assembly following the procedure in the 1100 Prep Pump
8. Pump 200 mL of IPA through the pump before replacing the SST filter assembly.
9. Replace the SST filter assembly following the procedure in the 1100 Prep Pump Service/Reference
Manual.
10. Replace the seal wash pump cartridge following the procedure in the 1100 Prep Pump
11. Perform the EMPV cleaning procedure as described in the 1100 Prep Pump Service/Reference
Manual.
12. Perform the built-in leak test following the procedure in the 1100 Prep Pump Service/Reference
Manual.
Agilent 1100 Series
4.1.6 G1376A Capillary Pumps and G2226A Nano Pumps
document.
3. Remove and disassemble the pump head following the procedure in the 1100 Capillary or Nano
5. Exchange the pump seals following the procedure in the 1100 Capillary or Nano Pump
6. Reassemble the pump head assembly following the procedure in the 1100 Capillary or Nano Pump
7. Replace the purge valve frit, gold seal, and plastic cap following the procedure in the 1100 Capillary
or Nano Pump Service/Reference Manual (that is if the pump is used in “Normal Mode” and a
manual purge valve is installed).
8. Replace the outlet ball valve sieve, gold seal, and plastic cap as described in the 1100 Capillary or
Nano Pump Service/Reference Manual.
9. Change the frit located in the filter in front of the EMPV.
10. Perform the EMPV cleaning procedure following the instructions in Service Notes G1376-04A and
G1376-05.
11. Prepare a bottle of isopropanol and perform the seal wear-in procedure as defined in the
1100 Capillary or Nano Pump Service/Reference Manual. The use of a restriction capillary (5022-
2159) is required.
Agilent 1100 Series
NOTE: This procedure is required for standard seals only (5063-6589). Performing this
procedure on the normal phase application seals (0905-1420) will damage them.
12. Using the same bottle of isopropanol, immerse solvent filter into the solvent and prime Channel A2
at 2500 μL/min for 10 minutes. This will require >25 mL of isopropanol.
13. Cap the outlet of the pump with a blanking nut (01080-83202).
14. Perform the built-in pressure test following the procedure in the 1100 Capillary or Nano Pump
− If you use the system in micro mode, perform the “Micro mode pressure test.”
− If you use the system in normal mode, perform the “Normal mode pressure test.”
Agilent 1100 Series
4.1.7 G1313A/29A Autosampler/G1387A Micro Autosampler/G2260A Preparative

Autosampler
document.
2. Change the needle and needle seat using the change needle function of the Control Module or
ChemStation maintenance functions. Follow the procedure in the 1100 Autosampler
3. Switch off power to the ALS.
4. Replace the rotor seal following the procedure in the 1100 Autosampler Service/Reference Manual.
5. Check that the chiller unit drain is positioned correctly (if applicable).
6. Purge ALS flow path with isopropanol for 5 minutes using the same channel as the previous
applicable pump maintenance section (4.1.4, 4.1.5, or 4.1.6).
7. If the column compartment contains a column switching valve, cap the outlet of the ALS with a
blanking nut. Otherwise, cap the outlet of the column compartment with the blanking nut (01080-
83202).
8. Run the built-in pressure test following the procedure in the 1100 Pump Service/Reference Manual.
may indicate an improperly assembled rotor seal or a loose fitting.
4.1.8 G1367A/G1377A micro well plate sampler and G2258A dual loop autosampler
document.
2. Switch off power to the ALS.
3. Change the needle and needle seat using the change needle function of the Control Module or
ChemStation maintenance functions. Follow the procedure in the 1100 Well Plate/Autosampler
Agilent 1100 Series
Service/Reference Manual. For the Dual Loop ALS G2258A, this procedure is performed only if
the needle is deformed or needle/seat is leaking (must be billed separately as a repair).
NOTE: The G2258A DLAs buffer loop capillary becomes shorter every time a needle is replaced.
After two to three replacements of the needle the buffer loop capillary must also be
exchanged (must be billed separately as a repair) when it has become too short to connect
the needle to it.
4. Inspect all G2258A DLA tubings and capillaries for visual signs of damage (kinks) and replace if
necessary (must be billed separately as a repair).
5. Replace the rotor seal following the procedure in the 1100 Series Well Plate Sampler & Micro Well
Plate Sampler Service/Reference Manual.
6. Replace the peristaltic pump cartridge following the procedure in the 1100 Series Well Plate
Sampler & Micro Well Plate Sampler Service/Reference Manual.
8. Purge ALS flow path with isopropanol for 5 minutes using the same channel used in section 4.1.4.
9. If the column compartment contains a column switching valve, cap the outlet of the ALS with a
blanking nut. Otherwise, cap the outlet of the column compartment with the blanking nut (01080-
83202).
4.1.9 G1328A/B Manual Injection Valve/5065-9922 Preparative Manual Injection valve
document.
2. Replace the rotor seal following the procedure in the 1100 Series Manual Injector Service/Reference
Manual.
Agilent 1100 Series
3. Purge injector flow path with isopropanol for 5 minutes using the same channel as the previous
applicable pump maintenance section (4.1.4, 4.1.5, or 4.1.6).
4. If the column compartment contains a column switching valve, cap the outlet of the injector
(port 3) with a blanking nut. Otherwise, cap the outlet of the column compartment with the
blanking nut (01080-83202).
Agilent 1100 Series
4.1.10 G1316A Column Compartments
document.
2. If a column switching valve is installed, replace the rotor seal as specified in the 1100 Column
Compartment Service/Reference Manual.
3. Purge column compartment with isopropanol for 5 minutes using the same channel used in
section 4.1.4.
4. Cap the outlet of the column compartment with a blanking nut (01080-83202).
5. Run the built-in pressure test following the procedure in the Pump Service/Reference Manual.
7. Perform the thermostat test following the procedure in the ChemStation’s Diagnosis View and
attach results to the PM Checklist. If there is no ChemStation installed, create your own method on
the Control Module:
− Both Peltier elements must initially be equilibrated to 25 °C. This temperature is held for
12 seconds, and then the set point is changed to 20 °C. The time required to reach 20 °C is a
measure of the cooling efficiency of the Peltier elements.
− At 3.5 minutes, the set point is changed to 30 °C, and both elements begin heating. The time
required to reach 30 °C is a measure of heating efficiency.
− Upon completion of the test, the cooling and heating efficiencies of both Peltier elements must
be calculated and compared to the acceptance criteria: the cooling rate for each Peltier must be
greater than 2 oC/min, the heating rate for each Peltier must be greater than 3 oC/min.
• Initial set point: 25 oC, stop time 8.0 min

o
Timetable: Min C
0.20 25
0.21 20
3.50 20
3.51 30
Agilent 1100 Series
• Record the time t1, when the set point for cooling (20 oC) is reached, subtract
t0= 0.21 min. The cooling efficiency is = 5 oC / (t1 –t0)
• Record the time t3, when the set point for heating (30 oC) is reached, subtract
t2= 3.51 min. The heating efficiency is = 10 oC / (t3 –t2)
8. Purge system with HPLC grade water for 10 minutes.
Agilent 1100 Series
4.1.11 G1157A/G1158A/G1159A/G1160A/G1162A/G1163A 1100 Series Valves
document.
2. Replace the rotor seal (and stator face) as specified in the 1100 Series Valves Operator’s Manual.
3. Purge valve(s) with isopropanol for 5 minutes using the same channel used in section 4.1.4.
4. Cap the outlet of the valve(s) with a blanking nut (01080-83202).
5. Run the built-in pressure test following the procedure in the pump Service/Reference Manual.
7. Purge system with HPLC grade water for 10 minutes.
Agilent 1100 Series
4.1.12 G1314 A Variable Wavelength Detectors
1. Verify that the outlet of the flow cell is unrestricted and flows directly to the waste reservoir.
2. Remove the flow cell from the detector and establish 2 mL/min flow rate using HPLC grade water.
3. Observe flow cell for any sign of a leak.
4. Reinstall the flow cell.
5. Perform the holmium oxide test as detailed in the 1100 VWD Service/Reference Manual Guide.
Record the value in the Test Result box if this test passes.
− If this test fails, perform the wavelength calibration test also found in the 1100 VWD
Service/Reference Manual Guide.
− If the wavelength calibration test is successful rerun the holmium oxide test. Record result in
the Test Result box.
6. Perform the intensity test as specified in the 1100 VWD Service/Reference Manual and attach
results to the PM Checklist.
Agilent 1100 Series
4.1.13 G1315A/B/C Diode Array Detectors and G1365A/B/C Multiple Wavelength Detectors
2. Remove the flow cell from the detector and establish 2 mL/min flow rate using HPLC grade water.
5. Perform the filter test as specified in the 1100 DAD/MWD Service/Reference Manual and attach
6. Perform the dark current test as specified in the 1100 DAD/MWD Service/Reference Manual and
attach results to the PM Checklist.
7. Perform the holmium oxide test as detailed in the 1100 DAD/MWD Service/Reference Manual
Guide. Record if this test passes.
− If this test fails, perform the wavelength calibration test also found in the 1100 DAD/MWD
Reference Guide.
− If the wavelength calibration test is successful rerun the holmium oxide test. Record result in
the Test Result box.
8. Perform the intensity test as specified in the 1100 DAD/MWD Service/Reference Manual and attach
Agilent 1100 Series
4.1.14 G1321A Fluorescence Detectors
2. Remove the flow cell from the detector and establish 2mL/min flow rate using HPLC grade water.

Perform the lamp intensity test as specified in the 1100 FLD Service/Reference Manual and attach
results to PM Checklist. The test is successful and can be marked PASSED if the profile observed
is similar to the one documented in the FLD Service/Reference.
Note: If lamp intensity appears low, check the window on the lamp housing. Clean or replace the
quartz window.
5. Perform the wavelength verification test using the Raman band as specified in the 1100 FLD
Service/Reference Manual and attach results to PM Checklist. If this test fails, perform the
wavelength calibration test as specified in the 1100 FLD Service/Reference Manual.
4.1.15 G1362A Refractive Index Detectors
1. If not already performed, flush flow cell with water for 10 minutes.
NOTE: The instrument has to be switched to "Purge" for the flushing of both the reference and
the sample side of the flow cell.
2. Check the diode balance as specified in the 1100 RID Service/Reference Manual. Verify that the
value does not fall outside the range of –0.2 to +0.2. If value is outside the specified range, perform
the optical balance procedure as specified in the 1100 RID Service/Reference Manual. Verify that
the diode balance is –0.2 to +0.2 and record this value in the Test Result box of the PM Checklist.
Agilent 1100 Series
4.1.16 G1364A/B/C/D Fraction Collectors
1. This maintenance procedure requires the consumables found in 1. in the parts list at the end of
this document.
2. Replace the inlet/waste tubing assembly (G1364A/B/C) or the capillary assembly (G1364D) as
specified in the 1100 series Fraction Collectors Service/Reference Manual.
3. Replace the valve to the needle tubing assembly (not for G1364D) as specified in the 1100 Series
Fraction Collectors Service/Reference Manual.
Agilent 1100 Series
4.1.17 Service Review
1. If ChemStation is the instrument controller, attach all printouts from tests performed to the
PM Checklist.
2. If the instrument firmware was updated at the request of the Customer, complete the IQ Attachment
Protocol and append it to the instrument IQ.
3. Make a test injection of a dilute mixture of acetone in water with no column. One drop in a
2 mL vial of water should be sufficient. Use λ 265 nm, 4 nm BW, >0.01 min PW. Run time should
be 2 minutes or less. The presence of a peak will indicate that the system is plumbed correctly. IF
THE PM IS BEING PERFORMED IN ADVANCE OF AN OQ/PV, USE THE PROTOCOL AS A
GUIDE FOR THE CHECKOUT.
4. Prime system with Customer’s mobile phase. IF THE PM IS BEING PERFORMED IN

ADVANCE OF AN OQ/PV, USE THE PROTOCOL AS A GUIDE FOR THE CHECKOUT.
NOTE: Many Customers prefer that water be left on the system. Confer with the Customer prior
to loading Customer’s mobile phase.
5. Complete instrument logbook if available, stating that a PM was completed.
6. Affix a PM sticker to the 1100 system or the instrument logbook.
NOTE: Some Customers do not approve of the placement of a PM sticker on the instrument.
This is optional and only performed if the Customer approves.
7. Customer and Support Provider sign the PM Checklist indicating service was performed.
5. ACKNOWLEDGEMENTS
Tefzel® is a registered trademark of E.I. DuPont de Nemours Co., Inc.
Vespel® is a registered trademark of E.I. DuPont de Nemours Co., Inc.
PEEK™ is a trademark of Victrex PLC.
Agilent 1100 Series
6. PARTS REQUIREMENTS
Please Note: Any parts, that are not included in the parts lists given below, are not part of a
regular Preventive Maintenance Procedure, they are not included in the price of this Service.
If a system has a special setup/configuration that requires the use of additional or special parts for the
instrument service, then these parts must be ordered separately and billed as a repair.
If a customer requests additional service that is not listed in the procedures described above to be
performed at his/her instrumentation, then the additional service must be ordered separately and billed
as a repair. Additionally required parts must be ordered and billed separately.
G1310A/11A/12A – Pump
This maintenance procedure requires the following consumables:
G1310A/11A Isocratic / Quarternary Pumps kit G1310-68730, consists of:
2x Seals 5063-6589 Standard (Pack of 2)
Purge-Valve Assembly
1x PFTE Frit 01018-22707 (pack of 5)
1x Outlet Gold seal 5001-3707
1x Outlet Cap 5042-1346
Outlet Ball Valve Assembly
Agilent 1100 Series
G1312A Binary Pump kit G1312-68730, consists of:
4x Seals 2x 5063-6589 Standard (Pack of 2)
Purge-Valve Assembly
2x Sieve 5063-6505 (pack of 10, needed for binary pump, only)
G1310A/11A/12A Isocratic / Quarternary / Binary Pumps kit for SEALWASH G1310-68731,

consists of:
2x Wash Seal 0905-1175 and
2x Wash Seal Gasket 5062-2484 (pack of 6),
Seal wash items are needed only, if the seal wash option is installed.
The kit above is needed twice for a G1312A binary pump.
Agilent 1100 Series
G1361A – Preparative Pump
This maintenance procedure requires the following consumables for each pump (the parts are all
included in the G1361-68710 Prep. Pump PM kit):
Pump Head Assembly
4x Prep Flange Seals 5022-2188
Multi-Assy
1x Filter cup 3150-0942
1x SST Filter Assy 5022-2192
Seal wash pump
1x Cartridge 5042-8507
1x Tubing 0890-1764
2x Fittings 5042-6422
Agilent 1100 Series
G1376A – Capillary Pump or G2226A – Nano Pump
Pump Head Assembly
2x Seals 5063-6589 Standard (Pack of 2) or
Purge-Valve Assembly (if pump is to be used in Normal mode)
1x Outlet Cap 5062-2485 (pack of 4)
2x Sieve 5063-6505 (pack of 10)
2x Outlet Cap 5062-2485 (pack of 4)
Filter Assembly
1x Frit 5022-2185
Agilent 1100 Series
G1313A/29A – Autosampler, G1387A – Micro Autosampler and G2260A

Preparative Autosampler
For G1313A/29A (these parts are all included in the G1313-68730 Autosampler PM kit):
1x Rotor seal 0100-1853 (Vespel®)
1x Needle Seat G1313-87101 with 0.17mm ID capillary (std.)
1x Needle G1313-87201 (for 100 micro-liter head)
For G1387A
1x Needle Seat G1329-87101 with 0.10mm ID capillary (std.)
1x Needle G1329-80001
For G2260A
1x Rotor Seal and Stator Face kit 0101-1268 (PEEK®)
1x Needle Seat G2260-87101 with 0.50mm ID capillary
1x Needle G2260-87201
Agilent 1100 Series
G1367A Well Plate Sampler, G1377A Micro Well Plate Sampler and G2258A Dual
Loop Autosampler
For G1367A (these parts are all included in the G1367-68730 Wellplate Autosampler PM kit):
1x Needle seat G1367-87101
1x Needle G1367-87201
1x Peristaltic pump cartridge 5065-4445
1x nut for sample loop fitting 0100-2086
For G1377A
1x Needle G1377-87201
1x Peristaltic pump cartridge 5065-4445
For G2258A
1x Peristaltic pump cartridge 5065-4445 (with Pharmed tubing)
Agilent 1100 Series
Note: If the following parts replacements are necessary because the needle is deformed or if
needle/seat are leaking these are not covered by the PM and they MUST be BILLED
SEPARATELY as a REPAIR:
1x Needle G2258-87306
1x Fitting kit 2mm 5065-9950
Note: After a needle has been replaced several times, the buffer loop capillary will become too
short and will also have to be replaced:
1x buffer loop capillary G2258-87300
G1328A/B – Manual Injection Valve and 5065-9922 Prep. Manual Injection Valve
1x Rotor seal 0101-0623 (Vespel®) for analytical scale manual injection valve (G1328A/B) or
1x Rotor seal 0101-1233 (PEEK®)
for preparative scale manual injection valve 5065-9922.
Agilent 1100 Series
G1316A – Column Compartment
This maintenance procedure requires the following consumables only if a column-switching valve is
installed (G1316A #055 1100 Series 2PS/6PT or G1316A #057 1100 Series 2PS/10PT Valve options):
1x Rotor seal 0100-1854 (Tefzel®) for valve opt. #055 or
1x Rebuild Kit 0101-1359 (Rotor Seal and Stator Face) for valve option #057.
G1157A, G1158A, G1159A, G1160A, G1162A, G1163A - 1100 Series Valves
1x Rebuild kit 0101-1359 (Rotor seal and Stator Face) for G1157A
1x Rotor seal 0100-2087 for G1162A
1x Rotor seal 0101-1361 for G1163A
Agilent 1100 Series
Detectors: G1314A VWD, G1315A/B/C DAD and G1365A/B/C MWD, G1321A

FLD and G1362A RID
The maintenance procedure requires NO consumables except the G1321A which may require the
following part:-
Quartz Window 1000-0999.
G1364A/B/C/D – Fraction Collector
This maintenance procedure requires the following consumables.
Inlet/waste tubing and valve to needle tubing kit
G1364-68712 for AS fraction collectors G1364A #050 and G1364C or G1364-68711

for PS fraction collectors G1364A or G1364B).
Capillary assembly for G1364D Micro Fraction collector
G1364-87304 (25 micro-m ID) or
G1364-87305 (50 micro-m ID) or
G1364-87306 (100 micro-m ID).

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Agilent 1100 Series

Scope of Work – Preventive Maintenance

Preventive Maintenance for the Agilent 1100 Series Liquid Chromatograph

Scope of Work – Preventive Maintenance

3.2 Support Provider Responsibilities

− Restriction capillary (5022-2159) for seal wear-in procedure

− Blank nut (01080-83202) for pressure/leak test

2. Has access to the latest 1100 Firmware @ http://www.chem.agilent.com/scripts/cag_firmware.asp

Scope of Work – Preventive Maintenance

4. Completes all steps in the PM Checklist applicable to the instrument.

NOTE: The PM Checklist is a separate document.

1. Writes the date service was completed on the PM sticker.

2. Applies PM sticker to the instrument or instrument logbook.

5. Signs the PM Checklist.

4.1 PM Task Details

3. Customer saves all methods and sequence files prior to PM start.

Scope of Work – Preventive Maintenance

4. Complete the PM Checklist in the order given.

4.1.3 Liquid chromatograph system

1. Check firmware revisions of modules to be serviced:

Scope of Work – Preventive Maintenance

7. For tests performed, attach printed results to the PM Checklist.

Scope of Work – Preventive Maintenance

4.1.4 G1310A/11A/12A Isocratic / Quaternary / Binary Pumps

2. Switch off power to the pump.

Scope of Work – Preventive Maintenance

15. Close purge valve.

Scope of Work – Preventive Maintenance

4.1.5 G1361A Preparative Pumps

2. Switch off power to the pump.

Scope of Work – Preventive Maintenance

4.1.6 G1376A Capillary Pumps and G2226A Nano Pumps

2. Switch off power to the pump.

9. Change the frit located in the filter in front of the EMPV.

Scope of Work – Preventive Maintenance

Scope of Work – Preventive Maintenance

4.1.7 G1313A/29A Autosampler/G1387A Micro Autosampler/G2260A Preparative

3. Switch off power to the ALS.

2. Switch off power to the ALS.

Scope of Work – Preventive Maintenance

4.1.9 G1328A/B Manual Injection Valve/5065-9922 Preparative Manual Injection valve

Scope of Work – Preventive Maintenance

Scope of Work – Preventive Maintenance

4.1.10 G1316A Column Compartments

• Initial set point: 25 oC, stop time 8.0 min

Scope of Work – Preventive Maintenance

8. Purge system with HPLC grade water for 10 minutes.

Scope of Work – Preventive Maintenance

4.1.11 G1157A/G1158A/G1159A/G1160A/G1162A/G1163A 1100 Series Valves

4. Cap the outlet of the valve(s) with a blanking nut (01080-83202).

7. Purge system with HPLC grade water for 10 minutes.

Scope of Work – Preventive Maintenance

4.1.12 G1314 A Variable Wavelength Detectors

3. Observe flow cell for any sign of a leak.

4. Reinstall the flow cell.

Scope of Work – Preventive Maintenance

3. Observe flow cell for any sign of a leak.

4. Reinstall the flow cell.

Scope of Work – Preventive Maintenance

4.1.14 G1321A Fluorescence Detectors

3. Observe flow cell for any sign of a leak.

4. Reinstall the flow cell.

4.1.15 G1362A Refractive Index Detectors