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Prepared by:

Mas, Mylyn L.
Ong, Dyan S.
PhCh 135-PHC
ASSAY OF LACTIC ACID

I. PRINCIPLES INVOLVED V. COMPUTATIONS


Type of Titration: Alkalimetric Residual with Blank 1. Pre-Lab Computation:
Type of Reaction: Neutralization Calculate for weight of Lactic Acid needed:
Indicator: Phenolphthalein TS Wt (g)= NT X VT X MWreagent
Endpoint: colorless  faint pink fx1000
Faint Pink  colorless = 1.0 N X (50.0 mL-20.0 mL) X 90.08g/mol
Titration Condition: Room Temperature 1 X 1000
= 2.7024 g of Lactic Acid
II. SCHEMATIC DIAGRAM (USP 35 p. 3630) Since volume is needed:
2.7024 g of Lactic Acid X ((%A.I /100 )Xspec.gr.)
2.5 mL Lactic Acid 2.7024 g X (90/100 X 1.2)
X = 2.919 mL of Lactic Acid to be used
 Add 50mL 1N NaOH 2. Post-Lab Computations
Volume of Excess Titrant Used (1.0 N NaOH); Nf= 1.0
 Boil for 20 minutes Volume Reading Trial 1 Trial 2
Initial Reading 0.00 mL 0.00 mL
Final Reading 50.00 mL 50.00 mL
 Add Phenolphthalein TS
Total Volume Used 50.00 mL 50.00 mL

 Titrate excess alkali with 1N Sulfuric Volume of Back Titrant Used (1.0 N H2SO4); Nf= 1.0
Acid TS Volume Reading Trial 1 Trial 2 Blank
Initial Reading 0.00 mL 0.00 mL 0.00 mL
*Perform a blank determination Final Reading 21.50 mL 21.65 mL 48.00 mL
Total Volume Used 21.50 mL 21.65 mL 48.00 mL
Reasons for Important Steps:
% A.I = [NT X Nf X (Vblank- Vactual)]backtitrant] X MWanalyte
 The solution is boiled for 20 minutes to f x 1000
sample wt (g)
speed up the reaction
Trial 1:
 The titration is done in room temperature [1.0 N X 1.0 N x (48 mL-21.50mL) x 90.08/1000)]
because many indicators give different 2.7024
indications at different temperatures. =88.33 %

Trial 2: [1.0 N X 1.0 N x (48 mL-21.65mL) x 90.08/1000)]


III. OFFICIAL REQUIREMENT
2.7024
Lactic Acid is a mixture of lactic acid (C3H6O3) and lactic =87.83%
acid lactate (C6H10O5) equivalent to a total of not less
than 88.0 percent and not more than 92.0 percent, by Average: 88.08 % of lactic acid contained in sample
weight, of C3H6O3. Acceptance criteria: 88.0 % - 92.0 %
PASSED

IV. CHEMICAL EQUATION % deviation b78etween trials:


|88.33% -87.83%|
[(88.33%+87.83%)/2]
+ NaOH  +H2O =0.568%
References:
Knevel, A. and DiGangi, F. (1977). Jenkins’ quantitative
pharmaceutical chemistry (7th ed.). USA: McGraw-Hill Inc.

C3H5O3H (aq) + NaOH(aq) C3H5O3Na(aq)+ H2O Skoog, D., West, D., Holler, F., Crouch, S. (2014). Fundamentals of
(lactic acid) (sodium lactate) analytical chemistry (9th ed.). California: Cengage Learning.
Factor: 1 since 1 mole of NaOH is needed to neutralize United States Pharmacopeial Convention, Inc. (2012). United States
1 mole of lactic acid. Pharmacopeia 35th Revision and National Formulary 30th Edition
Neutralization reaction: (p.3630). Maryland: USPCI.
2NaOH (aq) + H2SO4 (aq)  Na2SO4 (aq) + H2O