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ABSTRAK

Herdiana, Safira Putri. 2018. Uji Beda Penggunaan Salep Povidone Iodine
10% dan Salep Ekstrak Kunyit Terhadap Jumlah Fibroblast
Pada Proses Penyembuhan Luka Sayat Tikus Putih Jantan
(Rattus norvegicus strain wistar). Tugas Akhir, Fakultas
Kedokteran Universitas Muhammadiyah Malang. Pembimbing: (1)
dr. Mochammad Aleq Sander, M Kes., Sp.B., FINACS (2) dr. Gita
Sekar Prihanti MPd. Ked

Latar Belakang: Perawatan luka yang baik diperlukan dalam proses


penyembuhan luka. Salah satu metodenya adalah pemberian obat topikal.
Povidone iodine 10% mengandung antiseptik yang sering digunakan sebagai
pengobatan luka. Kunyit (Curcuma domestica) dapat digunakan untuk
perawatan luka alternatif karena mengandung komponen bioaktif seperti
saponin, tanin dan terpenoid yang berperan dalam proses penyembuhan luka
sebagai antiinflamasi, antibakteri dan dapat meningkatkan sintesis kolagen.

Tujuan: Membandingkan efektivitas salep ekstrak kunyit dengan salep


povidone iodine 10% terhadap jumlah fibroblast dalam proses penyembuhan
luka sayat tikus jantan putih (Rattus norvergicus ketegangan wistar).

Metode: True Experimental, Post Test Only Control Group Design. Sampel
dibagi 3 kelompok. Kelompok 1 kontrol positif (transparent dressing),
kelompok 2 (aplikasi salep povidone iodine 10%), kelompok 3 (aplikasi salep
ekstrak kunyit). Hewan uji diberi perlukaan sayatan pada punggung, dengan
ukuran ± 2 cm. Pengambilan sampel jaringan kulit dilakukan setelah 14 hari.
Untuk melihat jumlah fibroblast dilakukan pembuatan preparat dengan
pengecatan Hematoxylin Eosin.

Hasil Penelitian dan Diskusi: Uji normalitas menunjukkan bahwa distribusi


data normal dengan p>0.05. Uji homogenitas menunjukkan sig=0,065 yang
berarti varian data fibroblast hewan coba homogen. Hasil uji one way anova
menunjukkan signifikansi p=0,000 yaitu terdapat perbedaan bermakna
jumlah fibroblast antara kelompok kontrol dengan kelompok perlakuan.

Kesimpulan: Salep ekstrak kunyit menunjukkan peningkatan jumlah


fibroblast pada proses penyembuhan luka dibandingkan dengan salep
povidone iodine 10%

Kata Kunci: Fibroblast, salep ekstrak kunyit, salep povidone iodine 10%.

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ABSTRACT

Herdiana, Safira Putri. 2018. The Differences of using Povidone Iodine


10% ointment and Turmeric (Curcuma domestica) Extract
Ointment on the Number of Fibroblast on Incised Wound
Healing Process in White Male Rats (Rattus norvegicus strain
wistar). Thesis, Faculty of Medicine University of Muhammadiyah
Malang. Advisor (1) dr. Mochammad Aleq Sander, M Kes., Sp.B.,
FINACS (2) dr. Gita Sekar Prihanti MPd. Ked

Background: Adequate wound care is needed on wound-healing process.


Applying topical agent is one of the wound care methods. Povidone iodine
10% contain antiseptic is often use as treatment of wound. Turmeric
(Curcuma domestica) can be used to alternative wound care because it
contains bioactive components such as kurkumin, saponin, tanin, and
terpenoid that play a role in wound healing as anti inflammation, anti
bacterial agent and promote synthesis collagen.

Objective: To compare the effectivity of turmeric extract ointment with


povidone iodine 10% ointment on the number of fibroblast in healing process
of incised wound in white male wistar rats (Rattus norvergicus strain wistar).

Methods: True experimental and post-test only group design were used. The
sample were divided into three groups. They were positive control
(transparent dressing), group 2 (povidone iodine 10% ointment), group 3
(turmeric extract ointment). The animals test were given incision in the back
of the vertebrae, with incision size of ± 2 cm. Skin tissue sampling was
performed after 14 day. To see the number of fibroblast done making
preparations with Hematoxylin eosin staining.

Research Result and Discussion: Normality test showed that the


distribution of data were normal with p>0.05. Homogenity test showed
sig=0,065 which mean variant of fibroblast data of animal experiment are
homogenous. One Way Anova test result showed sig p=0,000 which mean
significant of fibroblast content between control group and treatment group.

Conclusion: Turmeric extract ointment showed an increase in the amount of


fibroblast were greater in the process of wound healing compared with
povidone iodine 10% ointment.

Key Terms: Fibroblast, turmeric extract ointment, povidone iodine 10%


ointment.

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