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Name: Sophia Aguirre Castro

Exercise 8: Chemical and Physical Processes of Digestion: Activity 4: Assessing Lipase Digestion of Fat Lab Report

Pre-lab Quiz Results


You scored 100% by answering 4 out of 4 questions correctly.

1. Which of the following is/are true of bile?


You correctly answered: b. It works by a physical process.

2. The substrate used in this simulation is


You correctly answered: c. vegetable oil.

3. When fatty acids are liberated by lipase, the pH


You correctly answered: b. decreases.

4. One of the products of the chemical digestion of lipids is


You correctly answered: d. fatty acids.

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Experiment Results
Predict Question:
Predict Question: Which tube do you think will have the highest lipase activity?
Your answer : c. tube 3

Stop & Think Questions:


What does the pH measure?
You correctly answered: d. both lipase activity and fatty acid release

Why is it difficult to detect whether lipase is active in tube 5?


You correctly answered: d. The pH is already very low, so a decrease in pH might be difficult to detect.

Experiment Data:

Tube No. Reagent 1 Reagent 2 Reagent 3 Reagent 4 Time Temp. pH


1 Lipase Vegetable oil Bile salts pH 7.0 60 37 6.21
2 Lipase Vegetable oil Water pH 7.0 60 37 6.72
3 Lipase Water Bile salts pH 9.0 60 37 9.00
4 Water Vegetable oil Bile salts pH 7.0 60 37 7.00
5 Lipase Vegetable oil Bile salts pH 2.0 60 37 2.00
6 Lipase Vegetable oil Bile salts pH 9.0 60 37 8.97

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Post-lab Quiz Results
You scored 100% by answering 4 out of 4 questions correctly.

1. What is the product of lipase hydrolysis?


You correctly answered: d. fatty acids

2. From your results, which pH is ideal for pancreatic lipase digestion?


You correctly answered: b. pH 7.0

3. Which tube confirms that there is no lipase in bile salts or vegetable oil?
You correctly answered: c. tube 4

4. From your results, where (in theory) would pancreatic lipase be active?
You correctly answered: d. mouth and pancreas

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Review Sheet Results
1. Explain why you can't fully test the lipase activity in tube 5.
Your answer:
Debido a que el pH en el tubo de ensayo 5 ya es muy bajo, es difícil medir los cambios de pH que causaría la liberación de
ácidos grasos.

2. Which tube had the highest lipase activity? How well did the results compare with your prediction? Discuss possible
reasons why it may or may not have matched.
Your answer:
Elegí el enunciado equivocado y, según los resultados experimentales, la lipasa tenía la mayor actividad enzimática
cuando estaba más cerca del pH en el intestino. El tubo de ensayo 1 tenía el pH más cercano al del intestino Delgado
por lo tanto aqui tendria mayor actividad

3. Explain why pancreatic lipase would be active in both the mouth and the pancreas.
Your answer:
La enzima lipasa pancreática tiene la actividad enzimática más alta en un entorno con pH 7. La cavidad oral tiene un pH
alrededor de 7 y el intestino delgado alrededor de 8, por lo que la enzima funcionaría en ambos lugares.

4. Describe the process of bile emulsification of lipids and how it improves lipase activity.
Your answer:
Los productos grasos se separan en gotitas por sales biliares a través de un proceso de emulsificación que es físico. No
es químico, lo que favorece la actividad enzimática de la lipasa.

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