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Have general and overall supervision of all section of the laboratory and all aspects of laboratory work. 2. Have general supervision of conduct of all laboratory personnel. 3. Have general supervision of all quality assurance and quality control schemes in the laboratory. 4. Supervices and directs all analytical procedures done in the laboratory. 5. Responsible for quality of all laboratory test results. 6. Signs out all results. 7. Take care of all communications and reports. 8. Recommends and signs out for requisition of reagents,supplies and equipment. 9. Oversees the general flow and usage of reagents. 10. Administer the budgetary aspects of the laboratory. 11. Prepares and submits financial report and budget of the laboratory together with the section chiefs,chief medical technologist and senior medical technologist. 12. Interprets laboratory report results as need arises. 13. Answers referrals probably arising from the laboratory. 14. Supervices the regular check-up and testing of all equipment and reagents.
Directs protocol for preventive maintenance of equipment. . Supervices the recording.15. Formulate plans for improvement and upgrading of equipment and laboratory procedures. 17. 16.evaluation of precision and accuracy of methods utilized in the laboratory.
10. To see to it that all premises of the different section in the laboratory is neat and tidy. To do all washing and cleaning of glass wares. 14. 8. To perform all laboratory examinations in the different section with accuracy and precision by the use of quality control. Perform test available in the laboratory. 9. Abides by the standard operating procedures set by the laboratory. 7. To perform and additional task that may assigned by their superiors. Records and reports all examinations properly. 15. 12. To report all laboratory results in the laboratory result form with complete data of the patient.filling or secretarial jobs in the laboratory in the absence of a secretary. 6. To do all collection of blood specimens for the patient request for laboratory examinations.test tube. . To do all clerical. 3. Refers requisitions to accredited laboratories. 4. Processes requisitions sent to the laboratory. To put away in their proper places all cleaned glassware and test tubes.MEDICAL TECHNOLOGIST: 1.etc. 11. 13. To record all laboratory results in the record book provided for each section. 5. 2.and tubes in the absence of a laboratory aid. To be present in the laboratory at their time of duty.pipettes.
To perform other delegated additional responsibilities on rotation basis as follows: a. Performs interviews. b. Keeping of stock cards and inventory and notification of supplies that are low in stock. Preparation of reagents for routine use. g. e. Consumption reports and performance report(monthly). . f. c. Maintenance and cleanliness of the laboratory. Keeping all records and files alphabetically.16. d. Statiscal reports(monthly).
The stat request must be immediately hand-carried to the laboratory and handed to the medical technologist. Routine Tests: Test ordered as routine will be performed and reported the same day. 2. Stat request should be reserved for patients needing prompt service.TYPES OF TEST ORDERS: 1.This means all other work must be stopped while stat test is being run.No verbal order is acceptable. Stat Requests: Any request marked stat will get immediate attention.IT SHOULD NOT BE USED TO COMPENSATE FOR DELAY IN ORDERING. .
spills and disruption of treatment and disposal due to equipment failure or other problems. Monitoring. Employee s responsibilities Personnel shall comply with established policies and procedures. b. Responsibilities For Safe Waste Handling a. d.Employees should bring to the management s attention unsafe working conditions and identify opportunities for hazardous waste reduction.POLICY ON DISPOSAL OF WASTE MATERIAL: 1. Managerial Responsibilities Head or supervisors shall design a system for the handling of waste that provides for proper collection.Records of such training shall be documented and filed in the individual personnel chart. c.packing and labeling all waste requiring special handling. Education and Training Education and training all personnel and supervisors shall be required.Bench technologists shall be assigned the important task of segregating.transport. . Contingency Plan For Employee s Injuries and Disruption of Disposal There shall be written procedures for the management of employee s injuries.storage.quality control and record keeping.disposal.segregation.Managers should design a system that utilizes less hazardous materials wherever and whenever possible.
CLASSIFICATION OF GENERATED WASTE FROM BLOOD SERVICE FACILITY a.physical. Pathological Waste Consists of blood samples.broken glass. c.stored.scalpels. Hazardous Waste Hazardous waste are solid or combination of solid wastes which because of its quantity.concentration. any item made of hard plastic or other material that can cause a cut or puncture. Sharps Sharps include needles.2. d.blood clots and serum specimens.lancets. .food and kitchen waste.chemical or infectious characteristics may pose a substantial or potential threat to human health or to the environment when improperly treated. Infectious Waste Infectious waste include disposable substances that have been used or have contact with blood and/or other body fluids which may harbor or transmit pathogenic organisms.syringes. Non-Hazardous Waste Any substance which is not known to pose substantial potential hazards to human health or the environment such as paper and other office supplies. b. e.blades.transported or disposed of.
5% 5. Segreation Segregation at the point of waste generation for classification and bagging in appropriate waste disposal bags or containers shall be done by the personnel or housekeeping staff trained in waste management. Hydrogen peroxide 6% To maintain effectiveness. b. Ethyl alcohol/ethanol 70% 3. Treatment All hazardous waste shall be decontaminated by any of the following methods: d. Use of Personnel Protective Equipment Water resistant utility gloves rather than latex or vinyl gloves shall be used for handling hazardous waste.5% chlorine 2.3. c.1-0.the disinfectants must be properly stored and freshly prepared using proper dilution. Isoprophyl alcohol/isopropanol 70% 4. Formaldehyde 4% 6. . Chemical disinfection by soaking for a minimum of 8 hours using any of the following methods: 1. Sodium hypochlorite solution with 0. GUIDELINES ON WASTE DISPOSAL a. Glutaldehyde 2% 7. Polyvidone iodine 2.
transporter of the BSF waste. LABELING All waste disposal bags/containers shall be labeled with the following: a. c. . 5.location of waste disposal. d. if outside the BSF premises.d.scalpels.infectious waste BLACK GREEN . All sharps shall be segregated from other waste and contained in rigid.storage. Packaging Waste containers must be designed to maintain its integrity throughout handling.transportation and treatment.biodegradable waste SHARP CONTAINER for needles. if applicable.puncture resistant and properly labeled containers from the origin to the final disposal.non-infectious waste .etc. 4. The color coding shall be as follows: YELLOW . b.leak-proof. use of BIOHAZARD symbol for all hazardous waste.lancets. SEPARATION OF HAZARDOUS WASTE BY COLOR-CODING OF DISPOSAL BAG/ CONTAINERS WILL PREVENT ACCIDENTS OR UNNECESSARY EXPOSURE TO INFECTIOUS WASTE AND WILL FACILITATE WASTE DISPOSAL. name of originating or generating facility.
preferably outside the BSF building but within the BSF premises.fire protection.The temporary storage areas should have appropriate ventilation.security and containment system. If a temporary storage area is not available.6.route. Storage areas should be located at a designated place not accessible to the public.and ultimate destination and disposal of waste. . WASTE COLLECTION AND TRANSPORTATION Properly completed manifests and disposal records must be kept on file to document the transport.The storage are should be regularly treated with insecticides. TEMPORARY STORAGE AND ACCUMULATION All BSF waste should be disposed of within 48 hrs. 7.daily disposal should be enforced.rodenticides and/or disfectants.
If the reaction is + new tubes or new detergents should be used or the dishwashing or rinsing technique should be checked.(to contain) . These can be detected by running a non-reactive control at the same time with the samples to be tested. 2.contains the amount of solution marked in its outside.calibrated to deliver the amount of solution marked on the side of its barrel.C and T. T.GLASSWARES: Test tubes Pipettes-T. PIPETTES: Pipettes like thermometer are calibrated by different methods depending on their use 2 basic types of pipettes: 1. .D.D Droppers Thermometer TEST TUBES: Dust or improper detergents or inadequate rinsing may modify the tube surface to produce false reactions.C. (to deliver) . T.
C.after it is fully drained. pipette correctly. This weight is then divided by the specific gravity of the water at the measured temperature and the volume of water is calculated.a set of caps pre-weighed should be used to prevent leakage of water from the pipette during the weighing procedure. T.D. .Occasional pipettes may be marked for both calibrations: T.the tip should be placed against the well of the receptacle and the fluid allowed to drain by gravity alone. To use a T.the last drop should be expelled from the tip by a gentle puff of air. Correct amount of solution is dispersed into a previously weighed receptacle. Presence of ground ring around the top of the pipette signifies that the pipette is calibrated to be blown out after the fluid has been drained by gravity. The receptacle is weighed again and the difference due to the weight of the water. To obtain all the solution contained in it-it must be repeatedly rinsed to remove all the material on the inside. Same procedure except the pipette itself is weighed rather than a receptacle. For ease in handling.D.
. Pipettes are filled with this solution. 2. Distilled water is placed in a beaker and its temperature measured.How to calibrate: 1.
*this dropper when hold vertically deliver water droplets each of which weights between 45 and 55 mg.the same dropper of that reagent bottle should be used all throughout until that reagent is consumed. *the drop should be expressed from the dropper moderately slowly so that the chances are that on accurate sized drop will be formed.pipette dry pipette should be used for each dilutions. *for this reason and for consistency. if T.or a bore of varying size are both reasons to reject the thermometer. .D. if T. Before it is to be calibrated: check the thermometer if it is in good condition a. THERMOMETER: a. DROPPERS: The standardized dropper has been defined: *glass tube constricted to 3mm. *errors could be obtained when other droppers are used for that reagent or when reagents are transferred to another bottle which has another sized dropper.a particle of glass in the bore.In performing serial dilutions with pipettes: 1.C. the bore containing the mercury should be examined microscopically to see if is clean and regular. 2. pipette same pipette is used to make all dilutions. *the dropper should be held upright when in use.outside diameter at its end.
or by cooling it so that the mercury is drawn into a slight enlargement just above the bulb when it will automatically unite. to enhance rbc antigen-antibody reactions in vitro. most water baths have partial immersion thermometer. the bath should be calibrated initially using complete or total immersion thermometer. take the equivalent temperature noted on the attached partial immersion thermometer. . c.C of a simple water bath really mandatory.if it is small.This makes the Q. to separate red cells from other fluid and cellular contents in the container. 2. WATER BATH THERMOMETER: a. 3. 1. b. I f the mercury column is broken.this can be reunited by centrifuging the thermometer. CENTRIFUGE: Uses of centrifuge in serologic testing: 1. to wash cell suspensions.b. WATERBATH: Temperature check daily. Most serologic procedures have incubation period and are therefore temperature dependent.
Monitoring the centrifuge: 1. . timer should be checked every 3 months. 2. In this manner. 3.after adjustments or repairs and annually in routine use. 4. speed of revolution rarely changes. each centrifuge should be calibrated upon receipt. record permanently all observations in the quality assurance records or equipment maintenance logbook. a. mark on the outside of each centrifuge the optimum speed and time of centrifugation. REFRIGERATOR: Refrigerator for storage of reagents: *can be a household refrigerator running at approximately 8-10 c *best only for reagents in active use and all the other reagents be put in a second stand-by refrigerator. b.only a few reagents in active use will be subjected to this temperature changes during repeated opening of the refrigerator and also during performances of procedures when reagents are moved in and out of the refrigerator. check accuracy of both timer and rpm.
HUMAN CONTROL Methods to avoid bias: a.blind testing-coded specimen b.optical aids e.duplicate testing *use of 2 different test methods *or use of 2 different manufacturers *or 2 different lot numbers nof antisera from same manufacturer c.proper maintenance of mechanical equipment .centrifuge calibration c. PHYSICAL CONTROL a.adequate lighting d.daily temperature checks of: *water baths *refrigerators b.scrupulously clean glasswares f.QUALITY CONTROL: I.use of 2 technologists for then same test *blood typing DIRECT REVERSE II.
using reagents according to manufacturers directions.III. IV. c. REAGENT CONTROL a.maintenance of file of current dated reagent brochures.adequate studies of changes in methods or technic before those routine practice.storage of records as required b. ADHERENCE TO WRITTEN PROCEDURES AND POLICIES a. b.anti RH serum daily with RH (+) and RH (-) cells.serum check anti-A & anti-B sera daily with A cells and B cells. c. MAINTENANCE OF ADEQUATE RECORDS a.periodic review of methodology b. .grading recording serologic reactions rather than recording interpretation only. d.review of brochures packaged with antisera when reagents are put into use. V.
Do not use a view box to perform ABO blood grouping since ABO antibodies are cold-reacting antibodies.with an applicator stick.to the section marked A add one drop of anti A typing serum and mix gently.one section marked A and one section marked B 2. 3. 4.Observe for agglutination at room temperature.but completely.to the section marked B add one drop of anti B typing serum and mix as before.PROCEDURE-SLIDE METHOD ABO Specimen-well mixed anticoagulated blood PROCEDURE: 1. .place a drop of the blood in a glass slide which has been divided into sections.