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A. Background of the Study
This study aims to produce an alternative specimen stain for plant and animal
tissues. It also conceptualize a natural a specimen stain from Basella Rubra
(alugbati) that is inexpensive and whose sources are indigenous and abumdant.

The viewing of microscopic pant and animal tissues is possible through biological
stain. When applied, the specimens being viewed become more visible. Before
performing such experiments and observations of microscopic detail, it usually
starts in preparation of the material to facilitate the progress.

Most of the biological techniques use synthetic chemical compounds made from
substances from coal tar. Natural dyes are rarely use nowadays, that’s why the
researchers deal with the study of having the biological effect of the Basella
Rubra extract as specimen stain.

B. Statement of the Problem
This study seeks to find the biological effect of Basella Rubra (alugabati) extract
as specimen stain on plant and animal tissues. Specifically, it aims to answer the
following questions:
1. How many Basella Rubra extract measure in ML is needed to produce a
specimen stain?
2. How accurate is the specimen stain from the mixture of the Basella Rubra
and water?
3. To what extent is the expiration of the said product?

C. Significance of the Study
The researchers focus on conceptualizing a natural specimen stain form Basella
Rubra extract. This study is essential to help teachers, students, students, scientists
and people whose perceptive in environmental phenomena.

In this research, the Basella Rubra extract which are only considered as medicinal
plant will be used as alternative specimen stain on plant and animal tissues. Costly
materials are not needed.

Researchers will learn the use of Basella Rubra extract as specimen stain on plant
and animal tissues. Researchers will be able to produce a large percent of
specimen stain in just using the extract form Basella Rubra without any longer
process that other specimen stain undergo and costly materials that other
specimen stain contain.

D. Scope and Limitation
Researchers will only test the biological effect of Basella Rubra extract as
specimen stain on plant and animal tissues not on chemicals. Researchers will

Excellent source of calcium and iron. ovate or heart-shaped. a good substitute for spinach. tapering to a pointed tip with a cordate base. in hedges. from flesh aerial parts. Mycosphaerella arachidicolaand Fusarim Oxyporum. Studies: • Anthocyanins/Natural Food Colorant : Study of pigment extracted from fruits of spinach vine (B. Rubra) showed good stability with a potential as a natural food color. 5-29 cms long. use for fertility enhancement in women. branched. Stains and dyes are frequently used in biology and . ovoid or spherical. good source of vitamins A. smooth. C and D. rubefacient. Review of Related Literature A succulent. diuretic. mixed with butter. Distribution found in settled and cultivated areas. with a high roughage value.only use water and Basella Rubra extract for each treatment. Sap is applied to acne eruptions to reduce inflammations. Poultice of leaves use to redue local swelling. B and C. Folkloric roots are employed as rubefacient. ethanolic and petroleum ether extracts of the leaves of Basella Rubra exhibited antimicrobial activity against all test organisms except aeruginosa. stalkless. Researchers will be using three treatments. Spikes are axillary. B. Decoction of leaves used for its mild laxative effects. both measured in ml. Pulped leaves applied to boils and ulcers to hasten suppuration. each with different amount of Basella Rubra extract (measured in ml. 5-6 mm long and purple when mature. a popular leafy and stew vegetable. In Ayurveda. 5-29 cm long. is soothing and cooling when applied to burns and scalds. ulcers and as laxative in children and pregnant women. stalked. In Nigeria. Leaf-juice. Specimen Stain Staining is an auxiliary technique used in microscopy to enhance contrast in the microscopicimage. Stems are purplish or green. Sugared juice of leaves useful for catarrhal afflictions. Leaves are fleshy. sexual weakness. emollient. Uses nutritional common market product. The researchers will not add other chemicals or ingredients to the treatments. The green and purple cultivated varieties are preferable to the wild ones. Fruit is fleshy. skin diseases. spinacoside C and momordins IIb and IIc. In this study. • Antifungal : Study yielded two antifungal peptides with potent activity against Botrytis cinerea. • Antimicrobial : A study of aqueous. used for hemorrhages. laxative. study isolated Basellasaponins A. solitary. oleanane-type triterpenes oligoglycosides. researchers will tets to see if Basella Rubra extract can be used as specimen stain on plant and animal tisseus and how effective they will be. twining herbaceous vine. Both the young shoots and stems are eaten. The ethanolic responsible for the antimicrobial effect. several meters in length. Properties and constituents: demulcent.) mixed with 25ml of water. together with betavulgaroside 1.

Staining and fluorescent tagging can serve similar Methodology In order for the researchers to do the often with the aid of different microscopes.wikipedia. Bilogical staining is also used to mark cells in flow cytometry. it can also be used to study of morphology of other materials for example the lamellar structures of semi- crystalline polymers of the domain structures of block copolymers.25 ml water mixed with 100 ml alugbati extract And in order for the researchers to find out whether it is really effective as specimen stain. Data and Results First Treatment Second Treatment Third Treatment 25 ml Alugbati 50 ml Alugbati 100 ml Alugbati extract extract extract 25 ml water Plant and animal Plant and animal Plant and animal tissue was viewed tissue was viewed tissue was viewed clearly definably clearly. for example. and to flag proteins or nucleic acids in gel eletrophoresis. . http://www. we needed alugbati extract and some water as specimen stain. It was then pounded. the plant and animal tissue was viewed clearly with 25 ml Alugabti extract and 25 ml water. for instance) or organelles within individual cells. muscle fibers or connective tissues) cell populations (classifying different blood cells.stuartexhange.25 ml water mixed with 25 ml alugbati extract . In biochemistry. . we the tested each treatment to plant and animal tissue. lipids. with the use of mortar and pestle to acquire the extract in three different amounts of Basella Rubra (measured in ml) to create three different treatments.html http://en. Staining is not limited to biological materials. detailed and definably Based on the researcher’s experimentation: • In the first treatment. carbohydrates) dye to a substrate to qualify or quantify the presence of a specific compound. it involves adding a class-specific (DNA. Stains may be used to define and examine bulk tissues (highlighting.25 ml water mixed with 50 ml alugbati extract . medicine to highlight structures inbiological tissues for viewing. proteins.

Being composed of 25 ml water and 100 ml of Basella Rubra extract. being in larger amount than the water. the plant and animal tissue was viewed definably with 50 ml Alugbati extract and 25 ml water. • Of all the treatments we used.• In the second treatment. detailed and definably by using the Basella Rubra extract solution. • In the third treatment. having more extract of Basella Rubra in a treatment will make the view of plant and animal tissue clearly. the plant and animal tissue was viewed clearly. we found out that: • The view of plant and animal tissue is viewed clearly. Analysis of Data Based on our data and results. It is because of the substance present in Alugbati. Conclusion In our experimentation of conducting the study about the biological effect of Basella Rubra extract as specimen stain on plant and animal tissue. detailed and definably with 100 ml Alugbati extract and 25 ml water. we were able to fin gout that the plant and animal tissue is viewed clearly. So. detailed and definably if the amount of Basella Rubra extract used in treatment is greater than the amount of water. composed of 25 ml water and 100 ml Basella Rubra extract. the third treatment took the biological effect most accurate. • The treatments were all accurate in terms of viewing the tissue clearly. detailed and definably. . The viewing of plant and animal tissue would be more definably if the amount of Basella Rubra extract is dominant than the water. takes the biological effect immediately.