B.E.

(Biotechnology) Fourth Year-Seventh Semester (Syllabus) BIO701 Environmental Biotechnology
Total Number of lectures: 45 Maximum marks: 100

Note for the Paper setter: The question paper should be divided into Section A and Section B Total of 8 questions, 4 from section A and 4 from section B are to be set.. The students will be required to attempt 5 questions selecting at least 2 from each section. SECTION-A Introduction: Environmental Pollution, Sources and effects. Biodegradation (5) and

Bioremediation- definitions and examples.

Waste Water Treatment: Aerobic and Anaerobic waste water treatment. Kinetics for waste water treatment processes. Different type and design of Aerobic and Anaerobic reactors, Methanogenesis. Emerging biotechnological processes in waste water treatment. (18)

SECTION-B Solid Waste management: Biodegradation of pollutants, Treatment processes for solid waste, Use and Management, Bioremediation of contaminant lands. Hazardous wastes: Source management and safety. (10)

Microbial Leaching and Mining: Recovery of metals from solutions, microbes in petroleum extraction, microbial desulfurization of coal. (7)

Environmental Genetics: Degradative plasmids, release of genetically engineered microbes in environment. (5)

Practicals: To determine BOD & COD levels of the sample. Application of microbial approaches in the remediation of contaminated soils. Analysis of contaminated and bioremediated samples. Reference Books 1. Bruce E. Rittmann & Perry L. McCarty. Environmental Biotechnology: Principles & Applications (1st Edition), McGraw-Hill, 2000.

Burton Editor & H. direct microscopic count.Marks:100 Course duration: 45 Lectures of one hour each.2. Paper Code : BIO 702 Food Biotechnology Max.). Michael T. Gail Vance Civille & B. Young (Eds. titrable acidity in the milk sample. Note : The question paper should be divided into Section A and Section B Total of 8 questions. Thomas Carr. Pergamon Press. 1999. Madigan. CRC Press. Food value of Mushrooms. David Stensel Wastewater Engineering: Treatment and Reuse (4th Edition). Brock Biology of Microorganisms (10th Edition). Oxford. Determination of diacetyl. . 1-4): M. Sensory Evaluation Techniques (3rd Edition). 2002. John Martinko & Jack Parker. Enumeration and detection of food borne organisms. Y. Reference books 1. Comprehensive Biotechnology (Vol. Morten Meilgaard. 2002. George Tchobanoglous. Production of Fermented foods (3) SECTION-B Food types and their physical & chemical properties Food Spoilage Food Borne diseases Food Preservation Diagnosis of microbial contents of food: Classical & Molecular approach Food Biosensors (4) (3) (6) (5) (4) (2) Practicals: Microbiological Examination of food/s. Estimation of quality of milk-by dye reduction. 4 from section A and 4 from section B are to be set. McGraw-Hill. 3. Franklin L.. The students will be required to attempt 5 questions selecting at least 2 from each section SECTION-A History of Microorganisms in foods (2) Primary sources of Microorganisms in foods (3) Synopsis of common food borne bacteria. 4. Yeasts. fungi and yeasts (6) Incidence & Behaviour of Microorganisms in foods (3) Extrinsic & Intrinsic parameters of foods (4) Role and Significance of Microorganisms as Single cell proteins. Prentice Hall.

Oxford University Press. Food Microbiology. 2. Perseus Books Group. D. 1989. 1992. 1995. requirements and Techniques. Smith. Arlington. Germplasm storage: Introduction. . 3. Big Money. (5) In-Vitro Pollination and Fertilization: Introduction. and field performance (5) Introduction. Turpin Distribution Service Ltd. Scott & Mark R. UK. Inc. Daniel Charles. Foodborne Diseases. Academic Press. 4 from section A and 4 from section B are to be set.D. Lords of the Harvest: Biotech. CRC Press.. Academic Press. Banwart. Morphogenic potential of the embryo callus. Roberta H. Herts SG6 1HN. 2001. Synthetic seeds. (5) Section B Microsurgical experiments. AVI/ Van Nostrand Reinhold Publishing Co. Section A Introduction. (4) Tissue Culture Media and Cell Culturing. Short or Medium term storage. M. Adams.R. (5) Genetic engineering and production of pathogen free plants: Gene expression. (10) Reference Books: 1. BIO703 Plant Tissue Culture Total Number of lectures: 45 Maximum marks: 100 Note for the Paper setter: The question paper should be divided into Section A and Section B Total of 8 questions. commercial aspects. Terminology. Letchworth. AOAC. Blackhorse Road. 1990.2. Nigel W. Plant Biotechnology: The Genetic Manipulation of Plants (1st Edition). 2000. Plant Tissue Culture: Techniques and Experiments (2 Edition).Food Biotechnology: Techniques and Applications. long term Storage. 2nd ed. Fowler. Gauri Mittal. (5) Cellular Totipotency. 5. 1995. Cliver. 7. (6) Haploid Production: Zygotic Embryo Culture: Morphogenesis in the culture of seeds with partially differentiated embryos. and M. Strategies used to optimize product yield. Bacteriological Analytical Manual (BAM) (8th Edition). Applications. In-Vitro Pollination. Moss. Adrian Slater. and the Future of Food (1st Edition). Somatic Embryogenesis.O. genetic stability. practical applications. Food and Drug Administration. VA. George J. Basic Food Microbiology. The students will be required to attempt 5 questions selecting at least 2 from each section. 6.. 4. 2003.

(05) Spectrofluorimetry: Principle. proportional and scintillation counters. Introduction to Raman scattering. Heslop-Harrison. various techniques to determine crystal structure. overview of different class of compounds and their IR spectra. 4 from section A and 4 from section B are to be set. units of radioactivity measurement. basic instrumentation for TEM and SEM. their working principle and comparison with other scanning techniques. alternate mass separation techniques. ionization techniques.(05) Radioisotope techniques: radiotracers. single and double focusing. Experiments in Plant Tissue Culture (3rd Edition). solvent perturbation method and difference spectroscopy. instrumentation details.r. Interface of massspectra with liquid and gas chromatography (LC-MS and GC-MS)(05) Practicals: Different experiments to confirm spectroscopic laws. Roberts. population distribution of nuclei. single beam and double beam spectrophotometer. Dodds & Lorin W. biotechnology.(04) Infrared and Raman Spectroscopy: Principle. Cambridge University Press.t. practical demonstration of the above mentioned techniques. diamagnetic shielding. sample preparation for electron microscopy. marks: 100 Time:3 Hours Course Duration : 45 lectures of one hour each. (03) Atomic absorption Spectrophotometry: Principle.t. Plants from Test Tubes: An Introduction to Micropropagation (3rd Edition). J. free induction decay (FID).3. Note for the paper setter: The question paper should be divided into section A and section B. and prediction of NMR spectra on the basis of (n+ 1) rule for basic class of compounds. Timber Press. The students will be required to attempt 5 questions selecting at least 2 from each section. 1996. factors influencing the absorption spectra. John Kleyn & John G. John H. anisotropy. significance of vacuum. instrumentation details of a mass-spectrometer. 4. overview of empirical rules. Overview of Atomic force microscopy and tunneling microscopy w.time of flight and quadruple. . introduction to autoradiography and nuclear medicine. significance. significance and various details related to instrumentation. instrumentation. Bragg¶s law. Total of 8 questions. (05) Crystallography and X-ray diffraction: Introduction to x-ray and general theory and instrumentation.r. various applications of absorption spectroscopy w. Lydiane Kyte. Kleyn. various interferences in atomic absorption spectroscopy and applications.(06) Mass Spectroscopic techniques: Introduction to mass-spectroscopy.(08) Section-B Electron Microscopy: Transmission and scanning electron microscopy.(04) Nuclear Magnetic resonance: phenomena of resonance. Overview of electron spin resonance spectroscopy (ESR) and magnetic resonance imaging (MRI). 1995. BIO 704 Bioanalytical Techniques Max. instrumentation. Section-A Spectrophotometry(UV and Visible): Principle. chemical shift. factors deciding the spectra.

New York Spectroscopy of Biological Molecules: Modern trends( Ist Editon). : : : Principles and Techniques of Practical Biochemistry (5th Edition). Physical Biochemistry. Keith Wilson & John Walker : 2.A. Carmona. Kluwer Academic Publishers. William Kemp 3. Navarro. Freeman & Co. . Cambridge University Press. English Language Book Society & The Macmillan Press Ltd.2000. 1997. David Friefelder 4. R. Organic spectroscopy.Suggested Books: 1. P.