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Advanced Technique for Rapid Non-Destructive Analysis of Fish Feed Composition


A wide variety of well known experimental procedures currently adopted by food producers has a number of common disadvantages. These standard chemicalphysical tests are usually: time consuming demanding specially trained staff costly destructive for the sample require frequent calibration utilise toxic solvents can not be implemented on the production line Modern nuclear magnetic resonance (NMR) analysers offer a wide spectrum of extremely quick, non-destructive and precise applications. Thanks to the high automation of modern NMR instruments most routine tests can be performed by virtually anybody. A routine measuring procedure can literally be described as “push the button - get the answer”. BRUKER minispec mq series (Figure 1) is one of the most advanced and powerful NMR analysers currently available on the market. It offers a wide range of standard applications for experimental determination of fat, oil and moisture contents in various media. The instrument has an important advantage of being a fully self-adjusting system with no need for manual tuning of measurement parameters. At the same time more advanced users have a possibility of implementing new applications using a built-in programming language (ExpSpel). This makes the minispec mq series well suitable for research and development purposes as well. The device has been ISO 9001 certified and complies with the European measuring standards.

Figure 1 BRUKER minispec mq series (Bruker Optik GmbH, Germany) The quality of fish feed and the effectiveness of fish feed production process are important issues for both fish feed suppliers and fish farmers. A correct combination of fat, protein, carbohydrate and moisture contents in fish feed is crucial for achieving a desirable growth rate and other key characteristics of farmed fish. A fast at-line control of fish feed composition would give fish feed producers an advantage of more flexible control over the production, resulting in a more effective consumption of energy and raw material ingredients, increasing the production speed and improving the quality of the final product. The ability to do rapid corrections in the ongoing production, based on at-line analysis would substantially reduce rework and lowgrade production. This would in turn result in an overall positive economical effect in the fish feed production. Figure 2 schematically demonstrates a simplified fish feed production line with some important control points where the low field NMR equipment could be used for product quality control.

Protein. Germany) . Carbohydrate. fat and moisture in fish feed implemented in the minispec mq10 low field NMR analyser (Bruker Optik. Fat and Moisture in Fish Feed 2 Initial ingredients Figure 2 – Application of low field NMR in quality control during fish feed production Protein and Carbohydrate Fat+Water Fat Figure 3 User interface of an application for the determination of protein. carbohydrate. GmbH.

For protein and carbohydrate calibrations.veliyulin@sintef. Fat and Moisture in Fish Feed 3 • ANALYSIS OF FISH FEED batches supplied from different sources.Protein. fat and moisture contents. carbohydrate. precise. Dirdal. the instrument response has to be calibrated against a set of reference samples with known protein. carbohydrate. All the mentioned parameters are determined simultaneously in one measurement. Norway) and has proven to be Emil Veliyulin E-mail: emil. The low field NMR equipment requires no maintenance. A typical fish feed sample consists of about 30 or more pellets depending on their size. The new method is based on a combination of the wellknown “solid echo”. Reliable determination of the protein and carbohydrate content with this technique suggests that the fish feed recipe is Bruker Optik GmbH Silberstreifen D-76287.sintef. the reference sample set must contain the raw ingredients as well. The NMR instrument operates at proton frequency of 10 MHz and has a 40mm bore opening.minispec. NMR response is not sensitive to minor changes in the microscopic properties of the raw materials used in the production being directly proportional to the total quantity of the measured Phone: (+49) (721) 5161 141 Fax: (+49) (721) 5161 237 . Therefore. The new technique has a potential to be implemented for an automated on-line quality control of fish feed Phone: (+47) 73 55 13 56 Fax: (+47) 73 86 77 08 E-mail: minispec@bruker. fat and moisture content in fish feed has been developed and implemented in the Bruker minispec mq10 low field NMR analyser. The program user interface and a typical NMR response is shown in Figure 3.. No toxic solvents are needed and the analysis can be performed by ground-floor personnel. The present low field NMR technique can be further modified and customised to determine similar quality-related parameters in other types of fodder as well as in a wide range of dry food products. Prior to the measurements. A new low field NMR method for determination of protein. These obvious advantages make the low field NMR technique highly competitive with both traditional chemical analyses and Near Infrared Spectroscopy. NORWAY http://www. The method has been successfully tested in the research fish feed factory (EWOS Innovation Ltd. Carbohydrate. For more information contact: SINTEF Fisheries and Aquaculture N-7465 Trondheim. Origin or type of fish oil and other raw ingredients do not have any significant impact on the observed NMR signals. it is not necessary to recalibrate the instrument for new raw material • SUMMARY The new low field NMR technique allows a quick and non-destructive determination of the main constituents in fish feed. Rheinstetten. and robust. GERMANY http://www. “free induction decay” and “spin echo” NMR sequences.