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Effects of chemical fertilizer and organic fertilizer made of eggshells and coffee grounds on Solanum

melongena (Talong Tagalog)

[1]Danielle Given L. Acido | [2]Vea Tryxzia Lei C. Alejandrino | [3]Nandi Seraphine M. Borja | [4]Alexei Faye
Custodio | [5]Lynn Abigael B. Romero | [6]Alexis Blythe Tayao | [7]Genevieve Anne N. Galino
[1,2,3,4,5,6] Science, Technology, Engineering and Mathematics Senior High School, De La Salle Araneta University,
Salvador Araneta Campus, Victoneta Avenue, Potrero, Malabon, Philippines
[7] Faculty, College of Arts, Sciences and Technoloy and Senior High School Department, De La Salle Araneta
University, Salvador Araneta Campus, Victoneta Avenue, Potrero, Malabon, Philippines

daniellegivenacido@gmail.com | zyaa06@gmail.com | nadhineborja@gmail.com | alexeifayecustodio@gmail.com |


lynnbromero@gmail.com | alexis21tayao@gmail.com| genevieve.galino@dlsau.edu.ph

Abstract: Chemical fertilizer has been used ever since for commercial farming. Due to its fast-acting
attribute, it is encouraged to use chemical fertilizer in agriculture to ensure a bountiful yield and to avoid
scarcity when it comes to resources even though chemical fertilizer causes a lot of damage in the soil
and, usually, in the plants due to the chances that it may become a source of food poisoning. This study
aims to create an organic fertilizer that can be used for commercial farming. Utilization of eggshells and
used coffee grounds will be done in order to formulate an organic fertilizer and compare it with a
commercially bought chemical fertilizer. Growth parameters of Solanum melongena were measured
after administering the organic and chemical fertilizer respectively. However, the results show that the
said organic fertilizer is not as effective as the chemical fertilizer, but it shows potential pesticidal
properties. As such, extensive study regarding this occurrence is highly recommended.

Key Words: fertilizer, coffee grounds, eggshells, Solanum melongena

many types of fertilizers used in horticulture, and they


I. INTRODUCTION are mainly classified as organic fertilizers and
chemical fertilizers. (Scherer, 2000)
1.1 Background of the Study Chemical fertilizers are usually made of
Fertilizer is any material made of natural or synthetic substances that mainly enhance and fortify
chemical ingredients that is used in horticulture such the quality of plants. It focuses on improving the
as gardening and farming to aid in improving the nutrients within plants by combining the synthetic
structure of soil as well as the quality of fruits, forms of their primary macronutrients such as
vegetables, and other agricultural products. There are nitrogen, phosphorus and potassium. They mainly use
petroleum products and rocks as well as organic Phosphorus (P), and Potassium (K) that a plant should
sources as ingredients, then, the nutrients are refined have to survive, without theses, it is possible for a plant
to their pure state and stripped of the substances that to be infected with different diseases and will have low
control their availability and breakdown which rarely possibility to last long.
occurs in nature. (Buckler, 2017) Powdered eggshells are rich in calcium
Chemical fertilizers are mainly used in carbonate and can help increase the amount of CaCO3
agriculture and gardening due to its fast-acting effect in soils. Eggshells are rich in nitrogen, potassium,
and inexpensive nature; however, as these kinds of phosphorus, calcium and magnesium, and using
fertilizers are primarily made of fossil fuels, they pose milled eggshells as fertilizers can increase these
a great danger to the environment. Repetitive use can nutrients in vegetables and other crops. The water for
result to toxic buildup of chemicals such as arsenic, boiled eggs also contains the following nutrients:
cadmium and uranium in the soil and groundwater. It small amount of sodium which is unlikely to affect the
can also cause over fertilization that can kill plants and plant, phosphorus, a small concentration of
disrupt the entire ecosystem as well as change the pH magnesium, and 4 milligrams of potassium and
level of the soil that can upset the microbial calcium in just one egg. (Gillman & Dirr, 2008)
ecosystems, increase pests and release greenhouse Coffee as fertilizer can increase crop yield,
gases. stress resistance, overall product and soil quality. It
Organic fertilizers are types of fertilizer that also supplies both soil and plants with nitrogen,
are made of organic materials such as formerly living potassium and magnesium and increase the soil pH
materials, plants and animals waste and powdered level to slightly neutral. Each plant reacts to coffee
minerals. Examples of these organic fertilizers are ground differently from each other, few can give
animal manure like chicken droppings, cottonseed positive effect, and some will have a negative effect.
meal, bones, ground sea shells (crab, oyster, etc.) and Coffee has a chemical called allelopathic that can
cultivated microorganisms and derivatives. possibly inhibit the growth of some plant. (Nghia Khoi
Use of organic fertilizers are more advised as Nguyen, 2016)
they not only improve the quality of plants but also the Using these materials for an organic fertilizer
structure of the soil. they increase the ability of soil to is highly advisable as the amount of nutrients present
hold water and nutrients, resulting to stronger and in them won’t exceed the capacity of the plants thus
healthier plants compared to the results by chemical avoiding over fertilization. The CaCO3 in eggshells as
fertilizers, and due to it being a slow-release fertilizer, well as the nitrogen, potassium and magnesium in
it is very hard to over fertilize using organic fertilizers, coffee can improve soil structure, increasing its
thus there is little to no risk of toxic buildups of capacity to hold water and nutrients. Also, coffee and
chemicals and salts, making them environment- eggshells as fertilizers produce bio-fertilizers as their
friendly. Nevertheless, all fertilizers can be classified by-product, resulting too little to no pollutant for the
as NPK fertilizers. (Day, 2008) soil and groundwater (Kitou & Akuno, 1999).
NPK fertilizers consists of the most needed
and important nutrients for a plant. It has Nitrogen (N), 1.2 Problem Statement
This research aims to determine the different The researchers will be having 3 set-ups: a
effects of NPK chemical fertilizer and organic negative control (Control A) which will not receive
fertilizer in terms of plant quality and physical any sort of fertilizer treatment, a positive control
appearance. (Control B) which will be receiving a fertilizer
treatment using a 15-5-10 NPK compound fertilizer,
1.3 Limitations and Set-Up A in which the researchers will be using
This study is only limited on the effects of the the organic fertilizer they made. Along with this, there
fertilizer on plants in terms of its growth parameters. will be 2 replicates per set-up in case the original set-
As such, other effects that have no connection with the ups wilt.
effects of the fertilizer but are recorded won’t be The researchers' organic fertilizer will be
discussed extensively in this research study. made using eggshells and used coffee grounds. The
eggshells will be first boiled then milled into small
1.4 Objectives granules then dried. Then, the milled eggshells will be
The main objective of the study is to create mixed to granules of the used coffee grounds in a
an organic fertilizer made of coffee grounds and 50:50 ratios.
eggshells that can produce satisfactory results while The researchers will be using Solanum
being compared to a chemical fertilizer. Its purpose is melongena (Eggplant or Talong Tagalog) for this
also to produce a cheap organic fertilizer for research. The plant has been authenticated by National
commercial farming. Bureau of Plant Industry. The seedlings will be planted
1. Compare the effects of chemical synthetic using loam soil combined with cocopeat, chicken
fertilizers and organic recycled fertilizers to manure, garden soil, vermi compost and ipa, and these
plants plants will be nurtured in the same environment in the
2. Know an alternative for chemical fertilizers De La Salle Araneta University and will be arranged
in commercial farming. randomly. The plants will be watered once daily if the
weather is cool and twice daily for when the weather
1.2 Hypothesis is hot, and the fertilizer treatment will be done twice a
1. Null Hypothesis: There is no significant month (every 2 weeks). The amount of water may vary
difference between the growths of the three day by day due to the changes in the temperature in the
set-ups environment which will be identified by a brannan
2. Alternative Hypothesis: There is a significant thermometer. A tablespoon of the fertilizer treatment
difference in the growths of the three set-ups. will be introduced to the set-ups. (Mierzejewski, 2016)
Eggplants are perennial plants but are usually grown
II. MATERIALS AND METHOD as annual plants. Due to its characteristic as a tropical
plant, eggplants are usually planted between August
2.1 Set up and February; however, it can also be planted during
This study conducted by the researchers is an the temperate climates around September and
experimental research of the quantitative nature.
December as well as during the cooler climates around
October and November if there are no longer frosts. IV. STUDY DURATION
Eggplants are low maintenance plants. The
plant only needs to be watered once or twice daily The study conducted by the researchers
depending on the weather (cooler - once; hot - twice). started from 14th of November to 8th of December in
It can be placed under the sun or under the shade, and the year 2018 under the supervision of the assigned
it can thrive even without fertilizer treatments, but if Research adviser, Ms. Genevieve Anne N. Gallino.
the plant is to be subjected to fertilizer treatment, it is
recommended to use 15-5-10 compound fertilizer or V. RESULTS AND DISCUSSION
any organic fertilizer that can provide the needs of the
plant. 1 tablespoon of the fertilizer is enough for 15 5.1 Results
days. It should be applied around the plant and not so Solanum melongena, or widely known as
close to the roots. (Moult, 2015) 'Talong Tagalog' or simply 'eggplant' (other names
can be 'aubergine' or 'guinea squash'), is a tropical
2.2 Testing annual plant, closely related to tomato in the family
After the fertilizer treatment for both the of Solanaceae which are mainly grown for their
Control B and experimental set-up, the plants will edible fruits. Eggplants are considered as herbaceous
undergo a quality checklist addressing their growth plants that feature erect bushy and branching stem
parameters. that may or may not have spines. The stem of an
Here is the sample of the quality checklist for the eggplant can grow up to 1.5 m (4.9 ft) in length. The
plants: (CABI Crop Protection Compendium, 2010) leaves are simple, flat, coarsely lobed and mainly
• Height of the stem (cm) green in color. It can grow up to 10-20 cm (4-8 in) in
• Number of leaves length and 5-10 cm (2-4 in) in width. The flowers of
• Length of the leaves (cm) the plant are mainly a light purple that can grow 3-5

• Width of the leaves (cm) cm (1.2-2.0 in) in diameter. The fruit is a large,
fleshy, ovoid berry which vary in color: white, green,
yellow, purple and black. Talong Tagalog usually has
2.3 Statistical Testing
Then, the researchers will be using ANOVA a white fruit with purple stripes. The fruit has a

statistical testing to identify the significant differences glossy smooth skin as well as numerous seeds and

between the data to be collected from the quality can grow up to 40 cm (15.7 in) in length. (CABI

checklist. Crop Protection Compendium, 2010)


As for this study, the researchers have

III. STUDY LOCATION omitted some of the plant’s growth parameters due to
insufficient time and focused on these three instead:

The researchers conducted the set ups at the • Height of the Stem: 1.5 m or 150 cm (4.9 ft)

mini green house of De La Salle Araneta University • Length of the Leaves: 10 - 20 cm (4 - 8 in)
located in Basic Education Department garden. • Width of the Leaves: 5 - 10 cm (2 - 4 in)
11.42>9.55 thus reject the null hypothesis – there
As such, here are the measurements made by is a difference between the average width of leaves
the researchers regarding the eggplant’s growth: of the set ups

Number of Leaves
These data are then analyzed and compared
Negative Positive Experimental Set
with the use of ANOVA statistical testing. The
Control Control Up
researchers have an alpha value of 0.5 and a critical
3 4 4
value of 9.55 which is used as a baseline to whether
3 4 3
accept or reject the null hypothesis.
Height of the Stem
Here are the results of the statistical testing:
14.5 cm 17.4 cm 16.5 cm
Number of Leaves
17.4 cm 17.8 cm 16.4 cm
Sum of Degrees of Mean
F-value Average Length of Leaves
Squares Freedom Squares
7.1 cm 10 cm 7.8 cm
9 2/3 0.1 11.3
7.3 cm 8.8 cm 7.4 cm
11.3>9.55 thus reject the null hypothesis – there is
Average Width of Leaves
a difference between the number of leaves of the
5.1 cm 7.2 cm 5.4 cm
set ups
5.2 cm 6.5 cm 5.3 cm
Height of Stem
Sum of Degrees of Mean
F-value
Squares Freedom Squares
42.96 2/3 7 11.43
5.1.1 Negative Control
11.43>9.55 thus reject the null hypothesis – there
The negative controls barely grow a leaf
is a difference between the heights of stems of the
during the duration of the research. They have three
set ups
leaves at max, and their growth parameters are barely
Average Length of Leaves
far from the minimum range. Also, the negative
Sum of Degrees of Mean
F-value controls are the most susceptible to pest infestation.
Squares Freedom Squares
As can be seen at the first picture, pests have eaten
37.88 2/3 6.05 11.83
most of the leaves of the set up while the second
11.83>9.55 thus reject the null hypothesis – there
picture shows that one of the leaves of the plant has
is a difference between the average length of
been eaten as well.
leaves of the set ups
Average Width of Leaves
Sum of Degrees of Mean
F-value
Squares Freedom Squares
142.44 2/3 23.45 11.42
shows no signs of being victims to pest infestation.
The leaves are all intact, and there are no signs of the
plant being eaten in any manner.

Negative Control 1 Negative Control 2

5.1.2 Positive Control


As for the positive controls, there are
obvious changes in the plants. The plants from the
Experimental Set up 1 Experimental Set up 2
positive control have more leaves, taller stems as well
as longer and wider leaves than the negative set ups,
5.2 Discussion
showing that the chemical fertilizer used is fast-
NPK fertilizer is the fertilizer that consists the
acting (this picture is taken a week after the
primary needed nutrients for the plants’ growth. The
treatment); however, the positive controls are also
agriculture industry is relying on the use of NPK
prone to pest infestation though not at the same
Fertilizer to meet the global supply and make sure that
degree as with the negative control. The leaves are
the crops are in their best conditions. Without proper
still intact after the pest infestation but large chunks
giving of the nutrients needed to a plant may cause
of them are eaten.
different reactions such as the plant will not grow to
their best potential, will give less yields, and even be
more prone on infectious diseases. And the most
important nutrients that a plant will be needing are
Nitrogen (N), Phosphorus (P), and Potassium (K),
without these three, a plant will not survive at all.
Organic fertilizers come in different
Positive Control 2 specifications and kinds, usually are animal manure.
Positive Control 1
They usually contain plant nutrients in low
5.1.3 Experimental Set Up
concentrations. Many of these nutrients must be
The experimental set ups show little
converted into inorganic forms by soil bacteria and
improvement after the fertilizer treatment. One of the
fungi before plants can use them, so they typically are
set ups has grown another leaf, and the plants are
more slowly released, especially during the cold
noticeably bigger and taller than those of the negative
weather when soil microbes are not as active. They
control; however, they are still considerably smaller
don’t make a crust on the soil as inorganic fertilizers
and shorter than those of the positive control. Another
sometimes do. They improve water movement into the
observation is that while the positive and negative
soil and, in time, add structure to the soil. Organics
controls are infested by pests, the experimental set up
feed beneficial microbes, making the soil easier to Plants like beets, cabbage, spinach, and lettuce could
work. (Feeco, Carison, & Capitaine, 2017) be enhanced. Coffee grounds are more effective if it is
Thus, it has been said that organic farming is in the liquid form by soaking the coffee grounds in
more advantageous compared to conventional water for days rather than applying it directly to the
farming, may it be long-term or short-term. Using soil because it takes time to break down the
organic fertilizer can result to decline in global microorganisms. Coffee grounds are often used on
warming as they release little to no greenhouse gases plants that thrive on acidic soil like blueberries,
and groundwater pollution. It can also improve soil evergreen, azaleas, avocado; however, used grounds
quality and structure which improves the microbial are not that acidic because most of the acid leaches out
ecosystem within the soil, of the cup.
prevents toxic chemical buildup in soils and decrease As for the eggshells, it is stated that calcium
chemical residue in plants. (Nagavallemma, Wani, carbonate that can be found in eggshells makes plant
Lacroix, Padmaja, Vineela, Babu Rao, & Sahrawat, cell walls stronger. It also contains nitrogen,
2004 & Oehl, Sieverding, Mader, Dubios, Ineichen, magnesium, phosphorus, boron, copper, iron,
Boller, & Wiemken, 2004) manganese, molybdenum, sulfur, silicon, and zinc.
Coffee grounds contain 2% nitrogen, 0.3% Eggshells prevent the leaves on bean to be pale-
Phosphorus, and 3% potassium (Stephen, 1994) as colored, brown leaf tips on cabbages and kohlrabi, and
well as some micronutrients. Different plants respond blossom end rot on tomatoes, peppers, and eggplant.
differently to the coffee grounds in addition to their (Rajhel, 2014)
soil. Plants such as lettuce seem to benefit with coffee There is a similar article that did an
grounds but some plants like tomatoes, wheat, and experiment on spent coffee grounds and eggshells as a
ryegrass may have negative effect (Kitou & Yoshida, fertilizer for Okra which ended with positive results.
1997). The negative impact may be caused by Spent coffee grounds and eggshells can be used as an
chemicals in the coffee called allelopathic chemicals, organic fertilizer directly to okra without the help of a
which inhibit the growth of some plant (Kitou & chemical fertilizer because it helps increase soil
Akuno, 1999). Nonetheless, some plants may benefit nutrients and okra yield by maintaining the soil pH,
from the coffee grounds, and as for the eggshells, the soil organic matter, soil nitrogen, soil bacteria, and
water from boiled eggshells (one egg) contain five fungi members that can make crops healthier and
nutrients; small amount sodium that is unlikely to help tolerant to negative environmental conditions like
or hurt the plants; phosphorus and magnesium that has nutrient deficiency, plant pests and pathogens.
low concentrations and 4 milligrams of calcium and Overall, their experiment regarding bio mixture of
potassium. Overall eggshell provides nutrition for the spent coffee ground and eggshells has a great potential
plants, but it shouldn’t be the only source of the in the development of organic and sustainable
fertilizer. (Gillman & Dirr, 2008) agriculture. (Nguyen, Nguyen, Do, & Lam, 2016)
Coffee grounds also contain many trace At face value, you might think that chemical
minerals aside from nitrogen, phosphorus, and fertilizers give farmers more control over their crop
potassium that has a good effect for some plants. production at the right price. Chemical fertilizers do
allow farmers to produce more and/or high-quality Enwall, Philippot and Hallin (2005),
crops in short term, but this type of treatment may lead mentioned that the organic fertilizers are long-term
to fewer or poor-quality crops in the long term. This is solutions because their nutrients are slowly released in
because of the intricacies of soil health. It can also the soil in a natural cycle, making them available to
make the topsoil acidic because nitrogen lowers the plants for longer time (over months or years).
pH of the soil. (Buckler, 2017) Moreover, it is a source of many essential elements,
The use of chemical fertilizers and organic even if each organic fertilizer has a different
fertilizers have both positive and negative effects on concentration of nutrients. Also, the essential
plant growth and the soil. Chemical fertilizers are microorganisms which build up in the soil humus
relatively inexpensive, have high nutrient contents, benefit greatly from the utilization of organic
and are rapidly taken up by plants; however, the use of fertilizer. (Alexander, 1980)
excess fertilizer can result in several problems, such as Hshuan (2006), reports that the nutrient
nutrient loss, surface water and groundwater supplies in the soil are balanced; however, proportions
contamination, soil acidification or basification, of organic fertilizers (animal wastes, compost) can
reductions in useful microbial communities, and provide the adequate amount the plant needs in regards
increased sensitivity to harmful insects. of nutrients such as N, P and K. Organic fertilizers
Organic fertilizer has a number of which are derived from animal sources, plant sources
shortcomings as well including low nutrient content, or compost are an ecological method, the requirement
slow decomposition, and different nutrient of carbon dioxide for its production is less than the
compositions depending on its organic materials production of chemical fertilizer.
compared to chemical fertilizers; however, organic The use of organic fertilizers, adding humus
fertilizer has multiple benefits due to its balanced to the soil, has the potential to keep positive charged
supply of nutrients, including micronutrients, cations and negative charged anions which are
increased soil nutrient availability due to increased soil available to the plant with the process of exchange
microbial activity, the decomposition of harmful capacity (Alice, 2008).
elements, soil structure improvements and root Rowel and Hadad (2002), stated that organic
development, and increased soil water availability. fertilizers modify soil’s structure and help improve the
In horticulture, a good reason for using capacity to hold water, the aeration, friability, and
organic fertilizers is that no matter where it is applied drainage. Moreover, most of the nutrients needed for
(gardens, lawns and even on greenhouses), it is the optimum plant growth are available on manures.
healthiest and best choice of fertilizer for animal, Humus serves as an effective buffer of soil pH that
children and family protection. It is not safe to come regulates the balance between acid and base in the soil
in touch with chemicals without properly protection; solution (Naramabuye, Haynes, and Modi, 2007).
however, organic fertilizers are safer and could bring In agricultural fields, organic manure that is
in healthier foods in healthier environment for all of produced from animal byproducts has been utilized to
us. overcome environmental contamination and plant
productivity reductions that result from the constant
utilization of chemical fertilizers. Recycling waste metal accumulation, water eutrophication and
from the livestock industry prevents environmental accumulation of nitrates. Such conditions must be
contamination and reduces treatment costs. At the considered in terms of air pollution since nitrogen and
same time, it promotes soil improvements and sulfur might accumulate in the atmosphere. This may
agricultural productivity. give rise to problems such as the greenhouse effect.
Compared to the use of chemical fertilizer Excessive use of chemical fertilizers in
treatment only, mixed chemical fertilizer and organic agriculture can result in many environmental problems
manure treatment in farmland rotating sorghum because of some fertilizers containing heavy metals
(Pennisetum glaucum) and wheat (Triticum aestivum), (eg. cadmium and chromium) and high concentrations
founded that organic manure increases the soil of radionuclides. Later these fertilizers’ agro-
concentrations of organic carbon, nitrogen, ecosystem constitutes the main source of heavy metals
phosphorus, and potassium, thus highlighting its and radionuclides in plants that can results in the
importance in tropical farmland which lacks organic accumulation of inorganic pollutants. Greenhouses
matter. aquaculture, especially large amounts of chemical
A study on tomatoes (Lycopersicon fertilizers used during the peak season can
esculentum) and corn (Zea mays) in acidic soil by dangerously pollute well water, especially water
Murmu et al (2013) showed a comparative assessment resources, crop production quantity and quality of
of conventional and organic nutrient management on product deteriorates. (Barlas, 2013)
crop growth and yield and soil fertility in tomato- Based on the individual data itself, there are
sweet corn production system found that organic significant differences between the set ups, and
manure increases crop productivity, nitrogen through the ANOVA statistical testing, the researchers
utilization efficiency, and soil health compared to can validate that there are significant differences
chemical fertilizer between the three set ups.
To meet the growing need for food, The researchers, as stated before, use the
agricultural land per unit area is required to achieve alpha value 0.5 and a critical value of 9.55 which shall
maximum efficiency and highest quality product. It is act as the baseline of the researchers on whether they
known that the nutrition of the plant is the one of the should or should not reject the null hypothesis. If the
most important factors to control agricultural F-value acquired from the statistical testing is lower
productivity and quality. Rates of nutrients in the soil than the critical value, then, the researchers are to
affects the quality of yield. accept the null hypothesis – there are no significant
In the permanent agricultural land, the soil differences between the three set ups; however, if the
will be very poor in nutrients, as a result, it will then F-value is higher than the critical value, then, the
become inefficient. Therefore, producers fertilize the researchers are to reject the null hypothesis and accept
soil, combat pests, conduct irrigation and other the alternative hypothesis – there are significant
agricultural measures that may aid in making the soil differences between the three set ups.
more efficient for farming. Excessive mindless Based on the tables above, in all 4
fertilization results in changes in soil salinity, heavy observations, the F-values exceed the critical value of
9.55. The first, the number of leaves, has an F-value of vegetable gardens, it cannot yield the same results as
11.3. The height of the stem has 11.43. The average the chemical fertilizer.
length of leaves has 11.83 while the average width of An alternative for the chemical fertilizer
leaves has 11.42 – all four exceed the critical value. As should be able to yield the same or greater results than
such, the null hypothesis is rejected by the researchers. it while causing minimal to no damage to the plant. It
should also be able to keep up with the demand for
VI. CONCLUSION food of the population, and because the eggshell coffee
ground organic fertilizer isn’t able to show such
Even with the limited time given, the results, the researchers can conclude that it cannot be
researchers are still able to compare the effects of an alternative for chemical fertilizer.
fertilizers on the plant. Researchers use a positive
control, the one with 15-5-10 NPK compound VII. RECOMMENDATIONS
fertilizer, a negative control: without any fertilizer, and As for the researchers' recommendations, it is
the set-up that used an organic fertilizer made of advised to conduct an NPK testing during the second
eggshells and coffee grounds. part of the study and compare the results between the
Researchers find out that both fertilizers have set-ups.
good and bad effects on their use. Positive control has Also, an increase in the number of organic
a much bigger leaves and longer stem. Its number of fertilizer treatment is recommended. Instead of doing
leaves is more than the negative control and the set up. it every two weeks, it is advised to do it once a week
The experimental set up almost has the same number to see if it is possible to use it as an alternative for
of leaves with the negative control, yet its stem length chemical fertilizer in commercial farming.
and measurement of the leaves are still greater. Then, it is advised to conduct an extensive
One of the most unexpected results of the study regarding the pesticide properties of eggshells
researchers is when the negative control is pestered, and coffee grounds.
and the leaves are eaten. This event happened after the
data gathering of the researchers, where thus few VIII. WORKING BIBLIOGRAPHY
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