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Seminar
On
INTRODUCTION
DEFINITION
Of
CHROMATOGRAPHY
MODES
Of
CHROMATOGRAPHY
Chromatography separation
may be achieved by
two basic techniques,
they are
1-Column chromatography
2-Thin-layer or Planer chromatography
PRINCIPLE
OF
CHROMATOGRAPHY
Types
Of
CHROMATOGRAPHY
In all the chromatography techniques the phenomena
of the adsorption or partition is involved. In the
adsorption, the binding of compounds to the surface of
solid phase (stationary phase) takes place, where as in the
partition the relatively solubility of the compound in two
phase, result in the partition of the compounds in two
phase.
Deepak Kr. Verma, 2010 Techniques in Biochemistry ABC-504
GLC: Principle & Application
RESET
Regulators Syringe/Sampler
Inlets
Detectors gas
system
Gas Carrier
Hydrogen
Air
Column
inlet
column
detector
data
system
There are two type of column used in the GLC, they are …
1- Packed Conventional Column
2- Capillary (open tubular) Column
DETECTOR
Used
In
GLC
Several types of detectors are in common used in the
conjunction with gas-liquid chromatography system…
APPLICATION
Of
CHROMATOGRAPHY