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ABSTRACT
1
status of the people in such ways that it
gives income and provides labor. Over
the years, the cost of farm inputs has
increased dramatically, therefore more
capital is needed. This created the
major problem of the farmers nowadays.
In addition, the continuous usage of
inorganic fertilizers and pesticides
caused poor soil condition, resulting to
lesser harvest and smaller the yield
(Farmer Production Guide on Pineapple).
Studies regarding Ananas
comosus revealed that mycorrhiza helps
the plant in absorbing more minerals
thus increases yield (S. Thamsurakul et Fig.2. Close up view of gathered Ananas comosus
al,_______). With the application of
Mycorrhiza, the pineapple yield has
increased and the efficacy of mineral The present study aimed to
fertilizer improved (S. Thamsurakul, et isolate and characterize them according
al., _____).The use of mycorrhizal to their mycelial growth rate and colony
symbioses to promote plant growth characteristics.
while reducing the inputs of fertilizers
and pesticides is one of the focuses of
most researches today (source?) Materials and Methods
Isolation of Mycorrhiza
from Pineapple Roots
2
Collection of Plant Materials Distinct fungal colonies that have
grown from the PDA plates were
Five samples of Ananas carefully isolated using an improvised
comosus were gathered at Sitio scalpel. The isolated mycelia were
Kurasay, Baretbet, Bagabag, Nueva transferred in another PDA plate so as
Vizcaya on August 28, 2009. Abiotic to come up with a pure culture of the
factors such as soil and air fungus. The cultures were periodically
temperatures were measured. Soil inspected to check their purity.
nutrients such as nitrogen, phosphorus
and potassium were also measured to Fungi Characterization
ascertain potentially available nutrients
for the plant. Isolates were characterized
according to mycelial growth of colony,
Isolation of Mycorrhiza colony margin and configuration, color,
and colony elevation. Diameter of the
The pineapples were dug from mycelial growth was determined
the soil using shovel. Its roots were
dipped in sterile distilled water to DMG = DV+DH
remove the fungal spores together with 2
some soil particles. The soil solutions Where:
were poured through the 3 layers of
DMG – diameter of the mycelial
sieve plates, which are no. 50, no.100,
growth
and no. 200 respectively (from widest
mesh to the finest). The filtrates were DV – measurement of the vertical
then collected in sterile beakers and diameter of the colony
transferred in sterile bottles with cover. DH - measurement of the horizontal
Samples of filtrate were taken from the diameter of the colony.
surface of the solutions using a sterile
pipette and were transferred in a petri Other colony characteristics were
dish with sterile distilled water determined through inspection of five-
afterwhich observation was done under day old cultures grown in selective PDA.
the stereomicroscope to see tadpole-like
bodies floating at the surface. When Results
tadpole-like bodies are present, these
were siphoned and inoculated in Fungal Characteristics
prepared PDA plates amended with
amoxicillin. An improvised procedure Mycelial Growth
was also employed wherein a sterile
loop was dipped at the upper surface of Table 1. Mycelial Growth of the
the filtrate after which the loop was Fungal Isolates
streaked on PDA plates. The plates
were incubated at a temperature of 30- Mean
350C for 24 hours. Day Isolate N Diameter
(mm)
1 A3 3 12.75
Pure Culture Production of Isolates A5 3 40.02
A1a 3 42.04
3
A1b 3 90.4
A3 3 22.66
A5 3 73.83
2 Table 2. Colony Characteristics of A3.
A1a 3 72.58
A1b 3 *
A3 3 30.5 Configuration Round with scalloped
margin
A5 3 *
3
A1a 3 *
Elevation Flat
A1b 3 *
A3 3 38.875
Margin Wooly
A5 3 *
4
A1a 3 *
Color Light brown
A1b 3 *
A3 3 51.70833
A5 3 *
5
A1a 3 *
A1b 3 *
Configuration Concentric
Elevation Hilly
Margin Wooly
Color White with dark green
Fig.4a. Day 5 of A3
4
Table 4. Colony characteristics of A52.
Configuration Concentric
Elevation Crateriform
Margin Branching
5
BIBLIOGRAPHY
R. M. Muchovej2, Importance of
Mycorrhizae for Agricultural
Crops.
_____________, Farmer
Production Guide on Pineapple.
S. Thamsurakul,et al.., Increasing
Pineapple Yield Using VA
Mycorrhiza Fungi.available online
at
http://science.jrank.org/pages/45
37/Mycorrhiza-Importance-
mycorrhizae.htm. date retrieved:
August 14, 2009.
Mycorrhiza.available online at
http://science.jrank.org/pages/45
37/Mycorrhiza-Importance-
mycorrhizae.htm.date retrived:
August 14, 2009.