A Research Paper Submitted in Partial Fulfillment of the Requirements in Research II

John Paulo A. Samin Project Leader

Agustin, Rezel S. Macaraeg, Jollena L. Mercado, Catherine May A. Paglinawan, Mary Pauline P. Pinili, Jomari G. Ruba, Rose Ann B. Proponents

FELERMA G. URBANO Research Adviser

08 February 2011



The use of inorganic fertilizers is widespread in the sector of agriculture inside the country. They played an important role in the good production of quality crops. Because they are expensive, some of our little farmers cannot afford to buy these products. Recently, they were found to bring hazards to the health and to the environment. This study generally aimed to determine the effect of indigenous microorganisms (IMO) in the growth and yield performance of tomato (Lycopersicon esculentum). One hundred five tomato plants (rosanna variety) were used in the study. They were randomly assigned into different treatments namely: Treatment 1(control; no application), Treatment 2 (30ml-IMO concoction), Treatment 3 (40mL-IMO concoction), Treatment 4 (50mL-IMO concoction), and Treatment 5(Inorganic fertilizers). Concoctions were mixed in a liter of water. These treatments were applied thrice in the experiment. Results were evaluated using the following parameters: plant growth, number of harvested fruits and weight of harvested fruits. Plant growth was obtained every ten days while total number and weight of fruits were recorded and weighed per treatments. Results showed that there is no significant difference between the treatments on plant growth. The number of harvested fruits was not significantly different from all the treatments. Moreover, ANOVA revealed that there was significant difference between the treatments. DMRT further revealed that T5, T4, T3, and T2 were comparable in the weight of fruits. This implies that IMO provides heavier fruits of tomato. According to Joan Puma, inorganic fertilizers serve as the easiest way of giving essential nutrients for the plants but microorganisms are the natural way of providing these nutrients. In terms of expense, IMO is comparable to the use of inorganic fertilizers. Hence, the result showed that the use of IMO in growing tomato plant can be an alternative to inorganic fertilizers in producing heavier fruits and promoting plant growth at lesser expense.



The researchers wish to express their deepest gratitude and warmest appreciation to the following people, who, in one way or another have contributed and inspired the researchers to the overall success of their undertakings: First of all, Mrs. Felerma G. Urbano, their research adviser who patiently taught and helped them in making their paper; Dr. Nenita Dela Cruz, who contributed a lot in the success of the paper and for sharing her precious time and her expertise to the researchers; Mr. Federico Pineda, a professor at the College of Arts and Sciences and a microbiologist, who gave the researchers additional information and facts which made their paper more competent; The DOST and Research Office staff who did not hesitate to help the researchers and answer all their questions which confused them along the process of making this research; Mrs. Zoraida Bartolome, librarian of the CLSU library, for her assistance in finding the needed references, like those information needed for the related literature of the study; Mrs. Christine Dalusong, who analyzed their raw data after the conduct to find the significance of their study;


Mrs. Priscila P. Esteban, MNHS computer teacher, for her patience and hospitality and in allowing them to use the computers to surf the web for further research; To their friends, who have unselfishly extended their efforts in giving them their ideas on what they know about research; Their greatest gratitude is extended to all the teachers for excusing them in class while doing their study and for meeting their consultant; To their parents who have always been very understanding and supportive both financially and emotionally and who at all times were behind them whenever they need encouragement; For those people the researchers failed to mention, with all humility the researchers are very grateful; And above all, to Almighty God, who perpetually shower the researchers with knowledge and strength to fulfill their every undertaking.

The Researchers



TITLE PAGE«««««««««««««««««««««««««««««..i ABSTRACT «««««««««««««««««««««««««««..«....ii ACKNOWLEDGEMENT««««««««««««««««««««..««.«iii TABLE OF CONTENTS ««««««««««««««««««««««««.v LIST OF TABLES «««««««««««««««««««««««««..«vii LIST OF APPENDIX TABLES «««««««««««««««...«««««viii INTRODUCTION Background of the Study ««««««««««««««««...«««««.1 Statement of the Problem «««««««««««««««...««««««.2 Hypotheses ««..«««««««««««««««««««««««««3 Significance of the Study ««««««««««««««««««««««4 Scope and Limitation «««««««««««««««««««««««..5 Review of Related Literature ««««««««««««««««««««..5 Review of Related Studies«««««««««««««««««««««.11 Definition of Terms««««««««««««««««««««««««16 Research Paradigm ««««««««««««««««««««««««18 METHODOLOGY IMO Collection «««««««««««««««««««««««««.19 Fermentation and Preparation of IMO ««««««««««««««««..19 Seedling Production «««««««««««««««««««««««..20 Experimental Design and Treatments ««««««««««««««««...20

Greenhouse Construction «««««««««««««««««««««..21 Transplanting ««««««««««««««««««««««««........21 Application of IMO Concoction and Inorganic Fertilizers ««««««««...21 Other Cultural Management Practices ««««««««««««««««..22 Harvesting «««««««««««««««««««««««««««.22 Gathering of Data ««««««««««««««««««««««««..23 Cost Analysis ««««««««««««««««««««««««««23 Statistical Analysis ««««««««««««««««««««««««23 Flow Diagram «««««««««««««««««««««««««...24 RESULTS AND DISCUSSION «««««««««««««««««««««.25 Average Growth of Tomato Plants «««««««««««««««««...25 Total Number and Weight of Harvested Tomato Fruits «««««««««...27 Cost Analysis «««««««««««««««««««««««««....28 CONCLUSIONS «««««««««««««««««««««««««««.30 RECOMMENDATIONS ««««««««««««««««««««««««31 BIBLIOGRAPHY ««««««««««««««««««««««««««...32 APPENDICES ««««««««««««««««««««««««««««.34 DOCUMENTATION «««««««««««««««««««««««««..39 CURRICULUM VITAE ««««««««««««««««««««««««.46



Table No. 1 2 3 4

Title Average growth of plants (in centimeters) Total Number of Fruits Harvested per Treatments Total weight of Harvested fruit per treatments (in grams) Comparison of expense between Concoction and fertilizers

Page no. 25 27 27 28



Table No. 1 2 3 4 5 6

Title Initial Height of tomato plants in cm Plant growth after 10 days Plant growth after 20 days Plant growth after 30 days Analysis of Variance in terms of Plant growth ANOVA for the number and weight of fruits harvested

Page no. 35 35 36 36 37 38


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