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By: Ms V. Krishnaveni Mr. G. Raj kumar
EXPERIMENTAL Material used Fabric Cotton fabric with the following specification is scoured and bleached prior to the application of finish.propyl . it is only in the recent couple of years several attempts have been made on finishing textiles with antimicrobial compounds. . The turbidity value was maintained over 187 even after being exposed to 15 consecutive home laundering conditions. Fabric Count Yarn Count GSM : : : 80 x 80 60s Combed X 60s Combed 180 Plain Type of weave : CHEMICAL USED Bio shield AM 500 which is an aqueous solution of silicon quaternary ammonium salt.Antimicrobial finish on textiles By: Ms. subjective evaluations of antimicrobial finish applied fabrics are analyzed. Anti microbial finish is a recent innovation in finishes. INTRODUCTION In the present day world most of us are very conscious about our hygiene and cleanliness. preventing diseases. Though the use of antimicrobials have been known for the decades. Obnoxious smell form the inner garments such as socks.dimethyl octadecyl ammonium chloride.6 ABSTRACT Bio shield AM 500 3. The antimicrobial treated sweat sample was tested for microbial activity and the turbidity was 189.propyl . Coimbatore . In this research. Department of Fashion Technology Kumara guru College of Technology. staining and degradation of textiles are some of the detrimental effects of bad microbes. Microbial infestation poses danger to both living and non living matters. G. but also good media for the growth of the microorganisms.(trimethoxy silyl). odour generating bacteria and mould fungi. The antimicrobial activities of the Bio shield AM 500 treated cotton fabrics were evaluated quantitatively against AATCC 147 by Gram staining and Turbidity test method. The consumers are now increasingly aware of the hygienic life style and there is a necessity and expectation for a wide range of textile products finished with antimicrobial properties. Clothing and textile materials are not only the carriers of microorganisms such as pathogenic bacteria. V. the key ingredient in it being 3 . This finish prevents the growth of bacteria and products finished in it have been proved environment friendly and health protecting. Raj kumar Lecturers.(trimethoxy silyl) . spread of diseases. It also prevents garments from unpleasant odour.dimethyl octadecyl ammonium chloride was applied to cotton fabrics by a cold pad-batch method in the presence of an alkaline catalyst to evaluate its use as a durable antimicrobial finish. Krishnaveni and Mr. The result of the antimicrobial activity is based on the standard test method AATCC 147 for evaluating antimicrobial effectiveness.
The material is given a cold wash for 5 minutes.3 gm 0. Gram staining After 24 hours of growth from nutrient broth. air dried and observed under microscope. Then it is decolorized with few drops of alcohol because large amount of alcohol makes even a gram positive cell to gram negative. Water Peptone Yeast Beef extract NaCL 100ml 0. Standard assessment test for anti microbial finish The subjective test is carried out based on the various standards set by the American Association for Testing Chemicals and Colorist AATCC 147. Finally the fabric samples are tested for antimicrobial activity as per the standard test methods. the growth suspension is taken using an inoculation needle and it is placed on a glass slide. The liquor ratio is 1:7. After adding alcohol. A broth is prepared with the following ingredients. The liquor ratio is 1:20. The following tests are carried out to find out the antimicrobial activity. Turbidity test Gram staining Preparation of Nutrient broth solution: Nutrient broth is a media which helps to grow bacteria with the necessary ingredients in a broth for the analysis of bacteria.2 gm 0. it is again washed with water and saffranin is added and it is kept for 1 min. 1.5 gm The solution is sterilized for 15 min at 1200C and it is allowed to cool. Gram staining 2. Then it is rinsed with distilled water and 2 drops of iodine is added and kept for 60 sec.2 gm 0. Bacteria stained by the gram method fall under two groups. 2% and 3% respectively at room temperature for 30 minutes with water. It is again washed with distilled water. Then it is treated with solutions like crystal violet and kept for 1 min. The material is hydro dried for 5 minutes. The pH is maintained at 7. Finish Application The pretreated cotton fabric is treated with bio shield AM 500 at three different concentrations 1%.ANTIMICROBIAL FINISH Pretreatment The material is treated with 2g/l acetic acid at 70 0C for 15 minutes with water. . The pH is maintained at 6. A smear is made and it is heat fixed. Then the fabric is immersed in the broth solution for 24 hours. Gram positive bacteria which retain violet colour and the Gram negative bacteria stained by sarafnin and hence appear pink in colour.
as the bacterial growth reduces with the increase in the concentration of the antimicrobial agent. the turbidity rate decreases as the bacterial growth reduces. The turbidity decreases. TABLE –1 Turbidity test Concentration (%) Untreated sample 1 % treated 2 % treated 3 % treated After 10 washes After 15 washes Sweat sample(un treated) Sweat sample (treated) Turbidity rate Sample1 283 256 232 189 187 186 456 192 Sample2 285 254 235 191 186 187 478 188 Sample3 280 253 230 187 187 190 443 190 Sample4 282 257 236 192 190 188 467 186 Sample5 279 255 234 188 188 187 458 189 .Turbidity test Turbidity test is the subjective evaluation test carried out to find the bacterial growth in the microscope .The result is predicted based on the turbidity of the solution which is determined by the amount of passage of light. Hence.
com Asian textile journal ‘March 2004’ P-. Fashion. product and services via promotional article.fibre2fashion. Review Of Textile Process. Technology.267 turbidity rate . Retail. follow this link: http://www.The findings of the study reveals that the 3 % concentration of anti microbial treated fabrics found to be very effective and can be used for hygenic and health care application.fibre2fashion. Butter Worth And Co. Technical Textile. Software and General please visit http://articles. Dyes & Chemicals. Bangalore. there are 183 microbes) which will not cause any problem to human beings.com To promote your company. but still there is very good scope for the textile researchers in this field. The bacteria found in the fabric are gram positive (turbidity value 183 indicates. P-33 To read more articles on Textile.org www.biogents. anti microbial finish on textiles has become essential in our day today life to live in fresh and hygienic atmosphere. Publishers Ltd. Even though many products have come.microbes. Further research work has to be carried out in finding out the genus and species of the organism. (1964).asp .45.com www.RESULTS AND DISCUSSION From the turbidity test results the bacterial reduction rate varies from 1 percent concentration 2 percent concentration 3 percent concentration . References www.com/services/article-writing-service/content-promotion-services. 47 Barxoclamo (July 2004) ‘Indian textile journal’ Butter Worth. CONCLUSION Apart from the industrial use.aatcc. Footwear & Jewellery. Machinery.183 turbidity rate There is a decrease in turbidity rate as the concentration increases. Apparel. Leather. house hold articles and baby care products. Industry.283 turbidity rate . The finish has excellent potential in various textile uses like inner wears.