Abstract

Ethnopharmacological relevance: Acmella uliginosa (Sw.) Cass. is a medicinal herbaceous plant that is commonly used by the Malay community in Malaysia to relieve pain often associated with mouth ulcers, toothache, sore throat, and stomach ache. Aim: The study was carried out to investigate the antiinflammatory effect of the methanolic extract of Acmella uliginosa (Sw.) Cass. flowers (MEAU) using carrageenan-induced paw edema test in mice. Materials & methods: Chemical (methanol, aspirin, absolute ethanol, carrageenan). The extract was given via oral administration at doses of 3, 10, 30 and 100mg/kg. A total of 36 mice were used and the mice were divided into 6 groups with 6 mice in each group. Results: It was demonstrated that intraperitoneal administration of MEAU at a dose of 3, 10, 30 and 100mg/kg produced significant dose-dependent inhibition of paw edema induced by carrageenan. Conclusion: The results from the present study appear to support the folkloric belief in the medicinal properties of Acmella uliginosa (Sw.) class which concluded that MEAU possessed anti-inflammatory effect against inflammation.

Introduction

Methodology
Mice (n=6 per group) receives oral treatment of A.uliginosa (3, 10, 30 and 100mg/kg) and Aspirin (ASA 100mg/kg, as positive control) or a similar volume of vehicle (10ml/kg) 30 min prior to subplantar injection of 0.02ml of ʎ-carrageenan (dissolved in 0.9% NaCl) into the left hind paw of each mouse. Paw volume was measured with a plethysmometer (Ulgo Basile, Itali) immediately prior to the injection of carrageenan and thereafter at an interval of 1 h for a period of 5 h

Acmella uliginosa, also known as ‘Subang Nenek’ or ‘Butang Baju Siti Fatimah’ locally is a flowering plant in the daisy family (Asteracae). It is annual or short-lived herb, spreads in low open places and grows well in moist soil (Wongsawatkul et al. 2008). Its flowers and leaves have pungent taste accompanied by tingling and numbness, and have been used as a spice for appetizers and as folk medicine for stammering, toothache, stomatitis and throat complaints. Plants of this genus often found applications in pharmaceuticals as an anti-toothache formulation for pain relief, swelling and gum infections, periodontosis and in mouthwashes. The present study was carried out to investigate the anti-inflammation effect of A.uliginosa flowers on acute inflammation in mouse model.

1.Oral treatment

2.Injecting carrageenan to the left hind paw

4. Measuring the volume of the left paw by using plethysmometer

3.Inflammation starting to occur at the left hind paw

(3-5h) is the result of release of kinins and mainly prostaglandins. Kedah Darul Aman. we need to have further study for the anti-inflammatory effect on chronic inflammation and find the active compound in A. 2. we also need to determine which mediators that are inhibited by this compound and lastly study the side effect of A. Munirah Mohd Mustaza1 . one of the important processes of inflammation. Mohd Roslan Sulaiman3. The carrageenan-induced paw edema formation is a classical model of acute inflammation and it is believed to involve a biphasic event. Preliminary Anti-Inflammatory Effect of Acmella uliginosa in Mice Norsafinah Mohd Tumiran1.uliginosa. 3. Selangor.uliginosa. Malaysia . 06300 Kuala Nerang. Kedah Darul Aman. Kolej MARA Kuala Nerang.Wan Ahamad Azran Wan Salleh2.  Besides that. Mohd Roslan Sulaiman Department of Biomedical Sciences.uliginosa does have antiinflammatory effect on acute inflammation  But. Nur Mahirah Liyana Dahalil1.induced paw edema in mice may be due to the suppression of the release of mediators responsible for inflammation including his- Acknowledgement : The authors would like to thank Faculty of Medicine and Health Sciences for providing the necessary support for our study * Address for correspondence Prof Dr. 1 1. 4. 2 Department of Unikl Kolej MARA Kuala Nerang. Faculty of Medicine and Health Sciences. Faculty of Medicine and Health Sciences. 3 Department of Biomedical Science. Nurfarah Diyanah Ahmad Kamal Ariffin1. The development of edema induced by carragenan is correlated with the early exudative stage of inflammation. In general. 06300 Kuala Nerang. Serdang. Universiti Putra Malaysia. Based on the present results.* Foundation in Science and Technology Royal College of Medicine Perak.Results and Discussions Conclusions  In summary. Hui Ming Ong3 .uliginosa on carragenan. the early phase (1-2h) is mediated by the release of histamine and serotonine. Universiti Putra Malaysia. 41400. While the second phase. it can be suggested that the inhibitory effect of the methanolic extract of A. this study provides convincing evidence that A. 43400 Serdang.

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