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Russo Enzyme Catalysis Lab report
Enzyme Activity Effects when tempreture rises When The catalysis is present
This shows what happens to the enzyme at certain Bubbles. states or when it is exposed to certain Nothing Happens because it environments or even denatures at extreme temp. when it comes in contact It Bubbles. with certain things.
Initial Reading Final Reading Baseline (which is the initial volume minus the final volume)
Class Data Time
This is the Catalyzed Decomposition 5ml measurements.
10 Seconds 30 Seconds 60 Seconds 120 Seconds 180 Seconds
Amount of H2O2 of Amount of H2O2 The Collection the class Decomposed in Decomposed in data which shows Trial 1 Trial 2 different seconds and measurements of the experiment that differs 1.43 1.03 depending on the 2.23 .83 accuracy of the lab. The trial two(2) of the 30 2.03 Second reaction was 1.83 reasonably low because 2.63 1.63 of some error that might have happened in the 2.43 2.83 experiment, such as the enzyme might have been weaker than usual, But overall, but observing the average amount that was
Average Amount of H2O2 Decompose d 1.23 1.53 1.93 2.13 2.63
it made sense that as the time increased. and substrate concentration on the reaction rate of an enzyme-catalyzed reaction. enzyme concentration. but it never fell. which means that more Hydrogen peroxide was decomposed at in the shortest amount of time. the amount of hydrogen peroxide also increased. The rate was not totally consistent.Class Data2 (Graph) Amount of Hydrogen Peroxide Decomposed (Trial 1 & 2) There were two trials used for each time ranging from ten (10) seconds to one hundred and eighty (180) seconds. The data and graph shows that during the 180 seconds. Notice that the amount of H2O2 decomposed increases with time. except for trial two (2) at thirty (30) seconds which the amount fell the lowest and this could be caused by errors in terms of Class Average data (Graph) By observing the outcome of the class’s average data. it was always increasing as time increases. . pH. the rate of reaction was the fastest. Another purpose of the lab was to explain how environmental factors affect the rate of enzyme-catalyzed reactions. Analysis The purpose of this lab was to observe and understand the effects of changes in temperature.
we were able to see a high rate of reaction toward the beginning of the reaction. the experiment could have given a better result. enzymes are very environmentally sensitive.Conclusion The catalase enzyme helped in the reaction of decomposing hydrogen peroxide into water and oxygen. . but it slowly leveled out when more of the H2O2 had decomposed. denaturing it and reducing the results of the reaction. Perhaps if the catalase was not out for as long. However. so our group was able to see direct effects on the reaction when the catalase solution was out for a period of time and possibly. This experiment could be improved by trying to be more accurate when measuring the reactants before they are added to the solution. As for the effect of time and the catalase on the rate of reaction.