Agricultural Inoculants Corp

RAPID COMPOSTING MICROBES
Ø A highly scientific biological formulation Ø Consist of 25 beneficial microorganisms: 18 Bacteria and 7 Fungi categorized as follows:
o Category 1: 7 Bacteria for Decomposition, Enzyme Production and Nutrients Transformation. Most Probable Number (MPN) Per Gram of Biomass = 1x106 up to 1x108. 3 Bacteria for Decomposition of Polysaccharides and Enzyme Production. Most Probable Number (MPN) Per Gram of Biomass = 1x106 up to 1x108. 3 Bacteria for Enhanced Decomposition, Compost “Sweetening” and Probiotics Production. Most Probable Number (MPN) Per Gram of Biomass = 1x105 up to 1x107. 5 Bacteria for Nitrogen Fixation and Nutrients Transformation. Most Probable Number (MPN) Per Gram of Biomass = 1x105 up to 1x106. 7 Fungi for Decomposition, Probotics Production and Nutrients Transformation. Most Probable Number (MPN) Per Gram of Biomass = 1x104 up to 1x106.

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Category 2:

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Category 3:

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Category 4:

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Category 5:

Ø Beneficial Microorganisms.

1.

Lactobacillus casei Propionibacterium sp. Methanobacterium forminicum Methanobacterium ruminantium Saccharomyces cerevisiae Aspergillus niger Aspergillus oryzae Cellulomonas fabia Penicillium notatum Humicola insolens Streptomyces thermophilus Thermoactinomyces vulgaris Thermospora curvata Bacillus stearothermophilus

2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. 13. 14.

15.

Thiobacillus thiooxidans Thiobacillus ferrooxidans Azotobacter vinelandii Nitrobacter winogradskyi Micorrhiza sp. Rhizobium japonicum Rhizobium leguminosarum Bacillus subtilis Bacillus licheniformis Bacillus polymyxa Rhizopus oligosporus

16. 17. 18. 19. 20. 21. 22. 23. 24. 25.

Bacillus stearothermophilus Donk
Class: Schizomycetes Order: Eubacteriacea Family: Bacillaceae
• • • • • Morphological Characteristics Rod shaped. Cell size: 0.6 to 1.0 µm in width and 1.5 to 5.0 µm in length Sporangium swollen, spherical in shape and at terminal position Usually gram positive The mol % of G+C of the DNA is 43 to 62 Physiological Characteristics Aerobic to facultatively anaerobic Chemoorganotroph Thermophilic Usually motile possessing peritrichous flagella. Growth Requirements Optimum temperature range for growth: 55-65 oC Optimum pH range for growth: 7.5 to 9.0 Source Compost heap Decomposing haystack Hot spring Commercial Use Production of enzymes such as catalase For rapid composting

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Cellulomonas fabia
Class: Schizomycetes Order: Eubacteriacea Family: Corynebacteriacea
Morphological Characteristics Short rods, exhibiting irregularities such as swollen ends or wedged shapes Small and pleomorphic rods, straight to angular or slightly curved with occasional beaded, clubbed, branched or coccoid cell Gram variable The mol % of G+C of the DNA is 58 to 69 Physiological Characteristics Chemoautotroph Capable of utilizing cellulose Produces yellow, water-insoluble pigment Motile by means of one or a few peritrichous flagella. Catalase positive Growth Requirements Optimum temperature range for growth: 35-55 oC Optimum pH range for growth: 6.5 to 7.0 Source Haystack and decaying wood Commercial Use For the breakdown of cellulosic materials and enhance the decomposition process such as composting. Production of enzymes e.g. cellulase and catalase

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Lactobacillus casei
Beijerinck
Class: Schizomycetes Order: Eubacteriales Family: Lactobacillaceae
• • Morphological Characteristics Rods often long and slender Cells variying from long and slender to short, often coryneform coccobacilli Chain formation common Non-sporing, some strains exhibit bipolar bodies Cell wall contains peptidoglycan Gram positive The mol%G + C of the DNA ranges from 32 to 53 Physiological Characteristics Facultative anaerobe or microaerophilic Catalase and cytochrome negative Pigmentation rare, if present, yellow or orange to rust or brick red Metabolism is fermentative Motility is uncommon; when present with peritrichous flagella Growth Requirements Optimum temperature range for growth: 30 to 40 0 C Optimum pH for growth: 4.5 to 6.2 Require carbohydrates as carbon and energy sources including nucleotides, amino acids and vitamins Growth media normally contains fermentable carbohydrate, peptones, meat and yeast extract Source Cattle manure and silage; diary processing plant sewer. Fermented vegetable foods. Commercial Use Production of lactic acid Fermentation of milk and other dairy products, e.g. cheese and yoghurt

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Methanobacterium forminicum
Class: Schizomycetes Order: Pseudomonadales Family: Spirillaceae

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Morphological Characteristics Spirally twisted rod, slightly curved, long to filamentous about 0.5 to 1.0 µm in width Has single flagellum or a tuft Endospores not formed Gram negative Physiological Characteristics Facultative anaerobe or photosynthetic Reproduces by binary fission Non-motile

Growth Requirements Optimum temperature range for growth: 30 to 40 0 C Optimum pH range for growth: 7.0 to 7.5 Source Waste lagoons. Garbage landfills Commercial Use Production of methane gas by reduction of carbon dioxide Reduction of sulfur to sulfide Enhanced composting process

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Methanobacterium ruminantium
Smith and Hungate Class: Schizomycetes Order: Pseudomonadales Family: Spirillaceae
• Morphological Characteristics Oval rods or cocci to short rods usually occuring in pairs or chains about 0.8 to 2.0 µm wide Possesses single flagellum or a tuft of polar flagella Cells are sometimes ellipsoidal or coccoid; 1.0 to 3.0 µm in length Gram positive The mol% of G+C of DNA is 30.6 Physiological Characteristics Anaerobic Reduces carbon dioxide to methane Reproduces by binary fission Non-motile Growth Requirements Optimum temperature range for growth: 37 to 40 0 C Optimum pH for growth: 7.5 to 8.5 Source Ruminants Anaerobic digesters Sewage sludge Commercial Use Production of methane gas through anaerobic digestion; reduces carbon dioxide to methane Enhanced composting of biodegradable matter

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Thiobacillus ferrooxidans
Temple and Colmer Class: Schizomycetes Order: Pseudomonadales Family: Thiobacteriaceae
Morphological Characteristics Cell size: 0.5 to 1.0 µm Colony size: 0.5 to 1.0 mm and round in shape with irregular margins Small rods usually single or in pairs The mol%G + C of the DNA is 58 to 59 Gram negative Physiological Characteristics Aerobic Obligate chemolithotroph Iron-oxidizing Motile with a single polar flagellum Growth Requirements Optimum temperature range for growth: 30 to 35 0C, but can grow between 10 to 37 0C pH range for growth: 1.3 to 4.5; optimum at about 2.5 Source Lagoons and biogas digesters Commercial Use Removal of foul odor in sewage treatment plants Conversion of ferrous ion to ferric salts

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Thiobacillus thiooxidans
Waksman & Joffe Class: Schizomycetes Order: Pseudomonadales Family: Thiobacteriaceae
Morphological Characteristics Short rods, single, paired or in short chains; 0.5x1.0 to 2.0 µm Cell size: small, rod shaped cells Colony size: 0.5 to 1.0 mm; colonies are transparent or whitish yellow Polarly flagellated rods Gram negative The mol%G + C of the DNA is 52 Physiological Characteristics Aerobic Obligate autotrophy Motile usually with single polar flagella Energy source is oxidation of sulfur Carbon source is carbon dioxide; nitrogen source is ammonia or inorganic nitrogen Growth Requirements Optimum temperature range for growth: 30 to 35 0C; growth range 10 to 37 0C pH range for growth: 1.3 to 4.5; Source Oxidation ponds of sewage plants Commercial Use Removal of foul odor in waste effluents e.g. utilizing sulfur from hydrogen sulfide Oxidizes sulfur to sulfide

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Bacillus subtilis Cohn
Class: Schizomycetes Order: Eubacteriales Family: Bacillaceae

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Morphological Characteristics Colonies are round or irregular; surface is dull or opaque Rod-shaped, seldom in chain Cell size: 0.7 to 0.8 µm in width and 2 to 3 µm in length Cytoplasm may be vacuolated The mol%G + C of the DNA is 41 to 47 Spores centrally located and ellipsoidal Gram negative Physiological Characteristics Chemoorganotroph Aerobic or facultatively anaerobic Strain uniform Can reduce HNO3 to HNO2 Hydrolyzes casein, gelatin and starch Excretes a yellowish green, fluorescent pigment

Growth Requirements Optimum temperature range: 36 ± 1 0C Optimum pH: 5.7 to 6.8 Growth media are normally nutrient broth or nutrient agar enrich with salt (NaCl) Source Soil in organic fertilizer plant Commercial Use Production of enzymes e.g. amylase, protease, lipase and cellulase Production of modified starches Production of meat tenderizers Desizing fibers

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Bacillus polymyxa
Prazmowski
Class: Schizomycetes Order: Eubacteriales Family: Bacillaceae
Morphological Characteristics Rod-shaped Cell size: 0.6 to 0.8 µm in width and 2.0 to 5.0 µm in length Spore shape: ellipsoidal Spore position is terminal Sporangium is swollen Colonies in nutrient agar thin after amoeboid spreading The mol%G + C of the DNA is 41 to 51 Gram positive Physiological Characteristics Chemoorganotroph Aerobic or facultatively anaerobic Can fix nitrogen under anaerobic conditions Can hydrolyze casein, gelatin and starch Growth Requirements Optimum temperature range: 37 ± 2 0C Optimum pH: 8.5 to 9.5 Growth media enriched with casein hydrolysate, ammonia, thiamine, biotin and nicotinic acid Source Decomposing starch Commercial Use Production of solvents and humectants Production of enzymes, e.g. protease, amylase, cellulase and catalase Production of organic acids from sugar Production of antibiotic e.g. polymyxin

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Bacillus licheniformis
Garner and Troy
Class: Schizomycetes Order: Eubacteriales Family: Bacillaceae
Morphological Characteristics Cell size: 0.6 to 0.8 µm in width and 1.5 to 3 µm in length Colonies on agar often opaque with dull to rough surface Rods often in chain Spore shape is ellipsoidal Spore position is centrally located Sporangium not swollen The mol%G + C of the DNA is 42 to 49 Gram positive Physiological Characteristics Chemoorganotroph Aerobic or facultatively anaerobic Can oxidize atmospheric nitrogen to HNO2 Catalase positive Motile by means of peritrichous flagella Growth Requirements Optimum temperature range: 37 to 45 0C Optimum pH: 6.8 to 7.0 Growth media enriched with peptone and KNO3 Source Livestock barns Compost heap Commercial Use Production of gluconic and other organic acids Production of enzymes, e.g. amylase, cellulase galactosidase and arginine dehydrolase Production of antibiotic e.g. bacitracin

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Streptomyces thermophilus
Class: Schizomycetes Order: Actinomycetales Family: Streptomycetaceae

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Morphological Characteristics Spores called conidia grow in spiral chains on aerial hypae which are 0.5 to 1.0 µm in diameter and of indefinite length Colony is convoluted Develops primary mycelium from which most of filaments arise Gram positive The mol%G + C of the DNA is 63 to 78

Growth Requirements Optimum temperature range: 55 to 65 0C Optimum pH: 6.5 to 8.5 Source Compost pile Decomposed sugar cane wastes e.g. mud press and bagasse Commercial Use Production of probiotics which can be processed into antibiotics e.g. streptomycin and tetracycline Production of polyene antibiotics from soymeal extract For rapid composting especially at the thermophilic range

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Physiological Characteristics Aerobic or microaerophilic Produces extracellular enzymes that permit utilization of polysaccharides, proteins and fats

Thermoactinomyces vulgaris Taiklinsky
Class: Schizomycetes Order: Actinomycetales Family: Streptomycetaceae
Morphological Characteristics Substrate mycelium is welldeveloped, branched, septate, 0.4 to 0.8 µm in diameter Spores formed singly or aerial and substrate hyphae, sessile or branched sporophores Aerial mycelium is 0.5 to 1.0 µm in diameter Gram positive, not acid-fast The mol%G + C of the DNA is 52 to 54.8 Physiological Characteristics Chemoorganotroph Aerobic some species are microaerophilic Produces by special method of sporulation

Growth Requirements Optimum temperature range: 55 to 65 0C Optimum pH: 7.5 to 8.5 Source Heating compost heap Decomposed mushroom substrate Commercial Use For rapid composting especially at the thermophilic range

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Thermonospora curvata
Teplyyakova Class: Shizomycetes Order: Actinomycetales Genus: Micromomosporacee

Morphological Characteristics
Hyphae do not fragment; each hyphae being 0.3-0.8 µm in diameter Conidia exist singly in pairs, or in short chains Spores are oval to round, 1.0 –1.2 by 1.2-1.5 µm, and are produced singly at the terminus of the specialized conidiophores Gram positive

Growth Requirements
Optimum temperature range for growth: 35-55oC Optimum pH range for growth: 7.5 - 8.5

Source
Compost heap Heating hay Decomposing manure

Physiological Characteristics
Heterotrophic; feeds on organic substrate Oligocarbophilic: can grow on limited carbon Obligate anaerobic thermophilic Microaerophilic to facultatively anaerobe

Commercial Use
For enhanced composting For decomposition of chitin, cellulose, hemicellulose and glucosides

Azotobacter vinelandii
Berjerinck
Class: Schizomycetes Order: Hyphomicrobiales Family: Azotobacteraceae
Morphological Characteristics Pleiomorphic: ovoid to rod-shape Cell size: Diameter is ≥ 2 µm; Length is ≥ 3 µm Colonies are generally smooth, glistening, opaque, low convex and viscid Flagellar arrangement is peritrichous Produces cyst Gram negative The mol%G + C of the DNA is from 63.2 to 67.5 Physiological Characteristics Obligate aerobe Heterotrophic Can fix nitrogen under atmospheric pressure; About 10 mg of atm N/g of glucose consumed Growth Requirements On nitrogen-free medium with sugar as carbon source, colonies appear within 48 hr at 30 0 C and reach a diameter of 2 – 6 mm in a week Optimum pH: 7.0 to 7.5 Source Waste effluent in lagoons Soil in the organic fertilizer plant Commercial Use Fixation of atmospheric nitrogen and converting it to NH4 –N

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Nitrosomonas europeae
Winogradskyi, 1892 Class: Schizomycetes Order: Pseudomonadales Family: Nitrobacteraceae
Morphological Characteristics Ellipsoidal or rod-shaped cells with intra-cytoplasmic membrane Cells occur singly or rarely in chains Reproduce by binary fission Cells rich in cytochromes which impart a yellowish to reddish color to cell suspension Gram negative The mol%G + C of the DNA is 45 to 54 Physiological Characteristics Aerobic but can also grow in low oxygen tension Chemolithotroph Can produce urease which permits them to use urea as a substrate Motile and some non-motile

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Growth Requirements Temperature range: 530oC pH range: 5.8 to 8.5 Major source of energy and reducing power is from oxidation of NH3 to HNO2 Source Soil in the organic fertilizer plant Commercial Use Oxidation of NH4-N to nitrite

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Nitrobacter winogradskyi
Winogradskyi, 1892 Class: Schizomycetes Order: Pseudomonadales Family:Nitrobacteraceae
Morphological Characteristics Cell size: 0.6 to 0.8 µm in diameter and 1.0 to 2.0 µm in length Short rod, often pear-shaped cells Reproduce by budding Cytomembranes occur in the form of a polar cap of flattened vesicles in the peripheral region of the cells Gram negative The mol%G + C of the DNA is 60 to 62 Physiological Characteristics Aerobic Facultative chemolithotroph Nitrogen-fixing Can oxidize HNO2 to HNO 3 Rarely motile and when motile, the cells have a single subterminal flagellum Growth Requirements Temperature range: 5 to 37 0C pH range: 6.5 to 8.5 Can grow heterotrophically in basal salt medium enriched with pyruvate, formate, acetate and glycerol as energy and carbon sources and with yeast extract, peptone, ammonia and nitrite sources Generation time: 8 to 14 hrs Source Soil in the organic fertilizer plant Commercial Use Oxidation of nitrites to nitrates

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Rhizobium japonicum
Class Schizomycetes Order: Eubactriales Family: Rhizobiaceae

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Morphological Characteristics Short rods measuring 0.5 to 1.0 µ in diameter and 1.0 to 3.0 µ in length Globular or ellipsoidal in shape Colonies are circular or rounded Gram negative The mol%G + C of the DNA is 55 to 65 Physiological Characteristics Aerobic Heterotrophic Motile when young, commonly changing to bacteroidal forms

Growth Requirements Optimum temperature range for growth is 25 0 C Optimum pH: 6.0 to 7.0 Growing best in media with extracts of yeast, malt and other plant materials Source Fermenting soyameal products Commercial Use For seed inoculation especially Leguminoseae For symbiotic nitrogenfixation on legume crops

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Rhizobium leguminosarum Bergersen
Class Schizomycetes Order: Eubactriales Family: Rhizobiaceae
Morphological Characteristics Cell size: 0.5 to 0.9 µm in diameter and 1.2 to 3.0 µm in length Short rods, and maybe globular or ellipsoidal Colonies are circular, convex, semi-translucent, raised and mucilagenous, usually 2 to 4 µm in diameter within 3 to 5 days on yeast-mannitol, mineral salt agar Commonly pleiomorphic Gram negative The mol%G + C of the DNA is 59 to 64 Physiological Characteristics Aerobic Heterotrophic and nonfermentative Non-spore forming Capable of fixing atmospheric nitrogen Motile with one polar or subpolar flagellum Growth Requirements Optimum temperature range: 25 to 30 0 C Optimum pH: 6.0 to 7.0 Grows rapidly on a wide variety of carbohydrates particularly mannitol, glucose, arabinose, fructose, sucrose and galactose Nitrogen requirements can be satisfied by HNO3 and ammonium salts Source Legume plants e.g. beans and peanuts and soybeans Commercial Use An inoculant to enhance the growth of seeds especially cereals and legumes Symbiotic fixation of atmospheric nitrogen

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Sporangium

Rhizopus oligosporus Saito
Class: Phycomycetes Order: Mucorales Genus: Rhizopus
Growth Requirements Optimum temperature range for growth: 20 to 30 0C Optimum pH range for growth is slightly acid: 5.6 Source Spoiled food in storage Decaying fruits and vegetables Commercial Use For seed inoculation For decomposition of polysaccharides e.g. mannan, xylan and galactan For production of proteolytic enzymes e.g. phytase

Sporangiospore

Stolon Rhizoid

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Morphological Characteristics Non-septate mycellia giving rise to straight sporangiophores Sporangiophores arise from rhizoids at nodes of the stolons Colunella is hemispherical Spores are oval, colorless or brown Rhizoids, nodes and stolons are present Physiological Characteristics Aerobic

Aspergillus niger van Tiegham
Class Deuteromycetes Order: Moniliales Genus: Aspergillus

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Morphological Characteristics Produces septate and branched mycelia Conidiophore (fertile hyphae) arises from an enlarged cell of the vegetative mycelium known as the foot cell and terminates in a swollen portion called vesicle Vesicle gives rise to sterigmata which may be single-layered or double-layered Color of the conidia which arise from the sterigmata maybe black, brown or green Physiological Characteristics Aerobic Heterotroph Capable of degrading cellulose and hemicellulose (e.g. mannan, xylan and galactan) as source of carbon Converts sugar to citric acid and gluconic acid

Growth Requirements
Optimum temperature range: 22 to 30 0C Optimum pH: 3.0 to 5.0 Growth media contain maltyeast-agar extract enriched with peptone, glucose and other sugars

Source
Pineapple pulp and decaying fruits

Commercial Use
In industrial fermentation, e.g. production of citric acid and gluconic acid Production of enzymes, e.g. sucrase and invertase Solubilizes insoluble phosphorus Decomposes protein, amino acids and other nitrogenous compounds releasing ammonia Generates ethylene which enhances root elongation, stimulates development of lateral roots and enhances seed germination Can produce pectic enzymes, e.g. pectin lyase Oxidizes powdered sulfur

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Aspergillus oryzae Cohn
Class: Deuteromycetes Order: Moniliales Genus: Aspergillus

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Morphological Characteristics Colonies varying from light greenish-yellow to deep yellowgreen Conidiophores are rough or pitted, colorless; 21 µm in diameter and 600 to 1700 µm in length Heads are hemispherical to columnar to sub-globose Sterigmata in 1 or 2 series Vesicles, 24 to 62 µm in diameter, are variable in form, from hemispherical to dome-shaped Conidia are globose, more or less smooth, size vary from 3 to 6 µm in length Sclerotia characteristic of many strains, generally greenish-brown to black Physiological Characteristics Aerobic Heterotroph

Growth Requirements Optimum temperature range: 25 to 30 0C Optimum pH: 4.0 to 5.0 Source Soy sauce; fermenting soybean Commercial Use Production of soy sauce Production of enzymes, e.g. protease and invertase or sucrase Production of amylase from corn meal and wheat bran

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Saccharomyces cerevisiae
Beijerinck
Class: Ascomycetes Family: Saccharomycetaceae Tribe: Saccharomyceteae
• Morphological Characteristics Unicellular: cells, 3 to 10 µm in diameter and 5 to 20 µm in length, are ellipsoidal or cylindrical, spheroidal, ovoid, occurring singly or in pairs Ascosporogenous showing multi-lateral budding; the asci contain 1 to 4 spheroidal ascospores Cell wall contains cytoplasm Has mitochondria (folded threads) measuring 0.3 to 1 µm in diameter and 3 µm in length Has vacuoles in the cytoplasm Reproduction is combined budding and fission Gram positive Physiological Characteristics Heterotroph Aerobic in early stages, but quickly become anaerobic Constituents of cells are 2 polysaccharides: glucan, 32% and mannan, 30% Also contains proteins, enzyme invertase and hydrolase Also contains lipids (8 to 13%) Contains multi-vitamins, e.g. thiamine, riboflavin, nicotinic acid, panthothenic acid, choline and inositol

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Growth Requirements Optimum temperature range: 20 to 30 0C Optimum pH: 3.5 to 3.8 Growth media contain malt and yeast extracts, peptone, glucose and agar Source Stale bread and fermenting cereal products Fermentation industries Commercial Use Conversion of sugar to ethyl alcohol Production of multivitamins mentioned above Beer manufacturing and other alcoholic beverages Production of fermented foods Production of glycerol from molasses

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Penicillium notatum
Class: Phycomycetes Order: Moniliales Genus: Penicillium

Morphological Characteristics Single-celled conidia are in chains developing at the end of the sterigmata arising from the metula of the coniophore Branched conidiophores arise from septated mycelium Physiological Characteristics Aerobic Sapphrophyte

Growth Requirements Optimum temperature range for growth: 20 to 30 0C Optimum pH range for growth is slightly acid: 5.6 Growth enhanced with peptones, plant extracts and agar Source Decomposing mushroom substrate Spoiling fruits and vegetables Commercial Use Production of penicillin from corn steep liquopr with sugar For production of proteolytic enzymes for efficient decomposition of proteinaceous substances emitting foul smell Also known to degrade pesticidal compounds

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Humicola insolens Cooney
Class: Ascomycetes Order: Myxomycetales Genus: Humicola

Morphological Characteristics crystal cells single-cell conidia in homologous chain filamentous fungi Physiological Characteristics
anaerobic or microaerobic

Growth Requirements
Optimum temp range: 45-55oC Can thrive well at 60-62oC Optimum pH range: 5.5-7.0 Grows best in minimal or slight oxygen

Commercial Use
Production of enzymes with many industrial uses For removal of inks, coatings and toners from recycled printed paper For wastewater treatment

heterothallic ascomycete thermophilic Source compost heap piles of agricultural and forestry products mushroom compost

For stonewashing of textile material

Mycorrhiza sp.
Class: Basidiomycetes Genus: Mycorrhizeae

Morphological Characteristics
Mycorrhizal roots are either ectotrophic in which the fungus hyphae form an extensive sheath around the outside of the root or endotrophic in which the fungus mycellium is embedded in the root tissue.

Growth Requirements
Optimum temperature range for growth: 20-35oC Optimum pH range for growth: 6.0 to 8.0 Utilizes simple carbohydrates for growth with one or two vitamins

Physiological Characteristics
Literally means “root fungus” Composed of long and short roots; short rods are dichotomously branched; long roots are usually unaffected Obligate symbionts Produces multi-stranded structures called rhizomorphs

Source
Forest trees especially mycorrhizal species

Commercial use
For enhancement of growth especially of non-mycorrhizal trees in reforestation projects or commercial tree planting For soil rehabilitation especially problem soils

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The Food Base
Category 1: Nutrients and Chelated Trace Elements

Carbon 2. Nitrogen 3. Phosphorus 4. Potassium 5. Calcium 6. Magnesium 7. Sulfur 8. Zinc 9. Iron 10. Boron
1. Category 2: 1. Enzymes

11. 12. 13. 14. 15. 16. 17. 18. 19. 20.

Copper Manganese Molybdenum Cobalt Sodium Chlorine Silicon Selenium Alumina Iodine

2. 3. 4. 5.
o Category 3: 1.

Cellulase Amylase Lipase Protease Lactase

6. 7. 8. 9. 10. 11.

Invertase Carboxylase Arginase Urease Phosphatase Sulfatase

Amino Acids

2. 3. 4.

Lysine Methionine Tryptophane Cystine

5. 6. 7. 8.

Cysteine Glycine Glutamic acid Leucine

Benefits of using AIC’s rapid composting inoculants
1. stabilize the putrescible matter in the organic waste 2. enhance the decomposition process. 3. exterminate pathogens and other harmful organism 4. eliminate weeds and unwanted foreign matter. 5. enrich the finished product nutritionally. 6. enhance the production of humus. 7. encourage the production of enzymes, probiotics as well as growth promoting substances. 8. It will produce a uniform and homogenous product phytotoxicity effect on plants. 9. it will remove foul odor. The finished product will posses an earthy smell.