Professional Documents
Culture Documents
, Tehri--249199 (UK)
BioReLab., Department of Zoology/Entomology, HNBGU (A Central University), Campus Badshahithaul Tehri
2. Department of Zoology, Government PG College, Kotdwara Garhwal (UK) INDIA
1.
Corresponding author: asokumr@gmail.com Cont: +919450501471
I NTRODUCTION
Biosorption Experiment:
Experiments of heavy metals biosorption were done in Erlenmeyer flasks containing 150 ml and 15.0 ± 1.0 mg of
cells. To ensure equilibrium, cells and waste were maintained in contact for 48 hours, under constant
agitation, at 30-350C ± 2ºC. After 48 hours, cells were separated from the medium and residual metal
concentrations were monitored by ICP-MS. The optimum pH and temperature maintained for the growth of
microorganisms in the batch culture. (Chatarzee et al 2001; Cybulski et al, 2003; Kumar et al 2010b).
R ESULTS
Tolerance of Microbes
The cultures conditions: Temperature (28–30oC), pH of 6.8 ± 0.2.
The metal concentration-0 mg/l (control) to 300mg/l
Streptomyces sp showed the tolerance for Cr and co-tolerance for Pb and Cu.
The control and optimization of bioremediation processes is a complex system of
many factors. They are
Existence of a microbial sps. capable of degrading the pollutants
availability of contaminants to the microbial population
Environment factors such as temperature
pH
Presence of oxygen or other electron acceptors
Nutrients
Microbes were allowed to grown in the media containing heavy metals
concentration and they were adopted for the concerned metals. Understanding
metal–microbe relationships has led to advances in bioremediation (Malik 2004;
Bruins et al. 2000).
Biosorption
capacities
depend on - M ETAL TOLERANCE
Metal
concentration
pH of the
solution Streptomyces sp.
Contact time
80
Ionic strength
Presence of 70
competitive ions.
Morley and Gadd 60
(1995) concluded 50
for fungal
biomass that the 40
different cell wall 70
polymers have 30
55
No. of Colonies
various functional
20
groups and
differing charge 10 25
distributions and 15 10 5
therefore 0
different metal- C 25 50 100 200 250
binding capacities
and affinities. Concentration of metal (mg/l)
B IOREMEDIATION A CTIVITY
Three metal Cu, Cr and Pd were sorbed by Streptomyces sp.
Table 1: Metal Site Sample CM (Cu) CMF (Cu) CM (Cr) CMF (Cr) CM (Pb) CMF (Pb)
conc. in the waste
samples (CM and Solid* ND ND 2.09 0.564 ND ND
HR
CMF). Liquid 3.90 3.198 8.56 2.140 0.858 0.582
Optimal
Solid ND ND 3.34 0.935 ND ND
conditions: DD
Temp. 300C ± Liquid 4.78 3.872 1.85 0.536 0.560 0.380
30C and pH 6.8
Solid ND ND 0.56 0.174 ND ND
±0.2. MR
Liquid 5.95 4.821 1.05 0.294 0.202 0.139
Sample were of Solid ND ND 0.96 0.288 ND ND
six cities: RK
HR: Haridwar Liquid 1.80 1.512 1.45 0.405 0.865 0.579
DD: Dehradun Solid ND ND 3.12 0.845 ND ND
MR: Mossorrie KR
Liquid ND ND 2.15 0.602 0.564 0.383
RK: Rishikesh
KR: Kashipur Solid ND ND 3.45 0.935 ND ND
RR: Roorkee
RR
Liquid 4.78 3.858 ND ND 0.562 0.382
Ashok
Kumar B IOACCUMULATION
Graph 2: Cu
Bioaccumulatio
n (mg/g of Cr
8
Biomass) by Pb
Streptomyces sp
6
(multi resistant).
4
They reduced
three metals
2
viz. Cr, Cu and
Pb. 0
Max. reduction
recorded by
acclimated
species was
chromium, it
was 72%.
Ashok
Kumar
D ISCUSSION
Microorganisms are intimately involved in metal biogeochemistry with a
variety of processes determining mobility and therefore, bioavailability.
T Y
f y e
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