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Biostats Lecture: Confidence Intervals Dr.

Holland (holland@um
Tues, March 15, 2011 (11am) Katherine Lubarsky (lubarske@umdn
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Confidence Intervals
Review
• All observations are a sample of possible outcomes, subject to random fluctuations.
• We are always uncertain, but make judgments based on the likelihood of the observations
that we see (“p values”).
• Decision rule: if p<alpha, too unlikely to consider as a random fluke -- reject null
hypothesis.
• Decision could be wrong:
o It IS a rare fluke → Type I error.
o You don’t pick up a difference or the sample doesn’t allow you to see a
o distinction → Type II error.
• To get the probability of a set of observations, use a probability distribution.
• Coin toss and patient survival (binomial examples).

Normal Distribution and Standard Deviation
Last lecture when we did the coin toss, we learned that the results of the tosses result in a
binomial distribution; the possibilities are ‘heads’ or ‘tails’ (2 possibilities - ‘bi’), and the
probability of getting certain results can be calculated simply by multiplication:
• The possibility of getting either heads or tails is .5 on each toss.
• To calculate the probability of getting a certain number of heads (or tails) in a row, multiply
.5 by the number of tosses. [Probably of 3 heads in a row = .5 x .5 x .5 = .125 (½ x ½ x ½ =
1/8)]

The normal distribution is commonly found in “normal” biological distributions such as height,
weight, blood pressures, etc. In a normal distribution, the values are continuous, and therefore,
the probability of values cannot be calculated by simply multiplying numbers the way it is done in
binomial distributions.

German statistician Carl Friedrich Gauss figured out a formula that related different spots along the
x-axis to different areas under the normal curve for which plugging in any x-axis number (the
observation) would give you the probability of occurrence of that number/observation (what the
likelihood is of getting that observation in that population). We must know the following terms:
mean, observation relative to the mean, and standard deviation.

The standard deviation (represented by σ - Greek letter sigma) is a measure of spread or
variability. It is standardized so that the same number of standard deviation units encompasses the
same proportion of observations in any normal distribution.
The normal curve is symmetrical and bell-shaped and
has a mean in the center (µ, mu), and there are
notations for µ+σ, µ+2σ, in both directions. The
number line below the x-axis denotes σ: +1 is 1 SD
above the mean (µ+σ), +2 is 2 SD above the mean
(µ+2σ), -1 is 1 SD below the mean (µ-σ), -2 is 2 SD
below the mean (µ-2σ).

Looking at the graph, you can see that in a normal
distribution, approx. 68% of the occurrences lie
within one standard deviation of the mean [+1 (µ+σ)
and -1 (µ-σ)]. Note that most values are very close to
the average, and few are at the extreme ends. If we

and so on and so forth. So if someone were to ask you. The mean +/. If you take the heights of 100 people from Newark. and we want to get the average height of . we find that 95% of values are within 2 SD. you would say that it is quite likely.).Biostats Lecture: Confidence Intervals Dr. 2 SD = 95%. in fact. thyroid concentration. March 15. This is exactly how labs generate normal ranges for all the things that are measured at labs (serum cholesterol. it occurs only 5% of the time. 2011 (11am) Katherine Lubarsky (lubarske@umdn Page 2 of 4 include more of the values (more of those at each end).] Example #1: Remember: mean +/. you can calculate the number of units of SD your observation is.2 SD includes 95% of the area. and 99% of values are within 3 SD. 3 SD = 99% . the taller and skinnier the curve. 3 SD = 99%. The diagram to the right shows various normal curves. you can tell how rare or surprising the observation is (what is the chance that I would have gotten this number?). then we can easily figure out the range of systolic blood pressures that includes 95% of the population: 1. and the highs and lows that show up in the abnormal column are that rare 5%. and when you divide that by σ.x is the observation you are interested in. Z-score The formula for calculating it is at the bottom of the above diagram . REMEMBER: 1 SD = 68%. This is true of all normal distributions. 95% likely. etc. The chances that one’s systolic bp is less than 80 or greater than 120 is rare. x-µ gives you the distance your observation is from the mean. Now we’re going to think about samples instead. What is the purpose of this? If given an observation (x). If we know (in a distribution of systolic blood pressures) µ=100 mmHg. it is bound to be different from the heights of another 100. but in each of the curves. Let’s take the average heights in successive samples of 100 from the Newark population. and the means will also be different. So far we have been talking about individual observations in a given population. 1 SD above and below the mean always encompasses 68% of the area under the curve (and 2 SD = 95%. 2 SD = 20 mm Hg (2 x 10) 3. and σ=10 mmHg. and that second set of data will be different than the following. what is the likelihood one’s systolic bp is 95. Similarly. Holland (holland@um Tues.see above. Mean =100 mm Hg 2. and you know the mean and SD of the distribution. The central 95% is considered normal.2 SD will give us our range: • (µ + 2 SD) 100 + 20 = 120 mmHg (our upper bound) • (µ – 2 SD) 100 – 20 = 80 mmHg (our lower bound) Answer: 95% of systolic bp observations are between 80 and 120 mmHg. Sample means (averages) taken repeatedly from the same population will almost always vary somewhat. Notice that the smaller the value of σ. The heights of each 100 measurements will be different from the other sets of 100. if there are 100 students in the lecture hall. If 1 SD = 10 mmHg.

The same rule regarding areas under the curve apply: 1 SE = 68%. If we had sampled only two student heights. a small sample size is not representative of the population. By the same token. then throw them back into the pool of 100. which results in a low average. There is just one difference between this and the normal distribution. one sample might include many short people. March 15. To the right is the distribution of means of samples. even if the underlying data are not normally distributed. 2 SE = 95%. We have now established that there is variability in samples.just understand the concept).it is the same because the means of samples are normally distributed. most of the means of those samples will be in the middle of the bell curve. This graph would therefore reflect the “normal frequency distribution” that we already saw .” and they themselves distribute normally. We take another 10 students and after measurement. In a normal distribution.Biostats Lecture: Confidence Intervals Dr. 3 SE = 99%. we may take a sample of size 10 (because it is easier and takes less time than measuring 100 heights). [We do this by random sampling. It wouldn’t even matter if we had the shortest or the tallest person included in that sample because the average is so stabilized by having 99 observations (which is 99% of the population) that the addition of an “atypical” observation doesn’t change the average very much. The distribution of means is normal. the accuracy of the estimation increases. Another sample may include many tall people. 2011 (11am) Katherine Lubarsky (lubarske@umdn Page 3 of 4 the class. So now we know that the means of samples are normally distributed – the second portion of the above mantra says that this will still be the case even if the underlying data is not normally distributed. but it is also important to realize that this variability is quite regular and predictable: The means of samples are normally distributed. Remember we are talking about means (averages) of samples. the more accurate the estimation. same students may be in the second sample – who knows ~ it is random. we talk about standard deviation. measure them. The central limit theorem proves that this is the case even if it may seem counterintuitive (we don’t need to know this. as sample size increases. average it. So again. most of the sample means will also be average. So if you take many samples (enough so that each sample becomes one observation on a graph). So we take 10 students. Let’s say in the second example we measured 99 out of 100 heights. Standard Error The standard error is the “error” that is involved when using a sample to gather data rather than using the entire population (which in most cases would be impossible to do). For example. and the average we would have calculated from them would not be accurate at all. which results in a high average. Samples will give you a . the same way that most people are of average height. What this means is that the mean of each sample becomes the new “observation.] Using these two examples we see that the larger the sample size. we use the term standard error (SE). it is very possible that we would have picked two students that were atypically short or tall. Holland (holland@um Tues. not specific pieces of data. In a distribution of samples’ means. But because most people are average height (by definition). calculate a different average from the first.

and the difference in exactness is calculated by the standard error. what is the range of sbp that includes 95% of mean bp that you’d find in samples? 1. 95% of the sample means are between 98 and 102 mmHg. and z becomes very small. or difference. take the sample mean (100) and add 2 SE (2 x 1) → 102 So. If the mean for samples of systolic bp = 100 mmHg. but it won’t be exact. This again reiterates the idea that the larger the sample size. 2011 (11am) Katherine Lubarsky (lubarske@umdn Page 4 of 4 representation of the population. the denominator becomes very large. Likewise with a small sample size (small n) z will be large .10 (SD of sample means) / square root of 100 (n) = 10 / 10 = 1 2. Holland (holland@um Tues. Note how the square root of n is in the denominator .Biostats Lecture: Confidence Intervals Dr. the SD = 10 mmHG. the more accurate the estimation. In other words. given the sample we drew and the variability associated with the size of the sample. “We are 95% certain that the real value is within these boundaries. if n is very large.the sample is so small that you can’t really get a sense of what the true population is. So in calculating the 95% CI (which is what we did above). To get the upper bound. That is the mathematical way of saying that with a very large sample size (n). To get the lower bound. The equation at the bottom of the diagram for ‘z’ is the shift. Because there is variability in samples we use a Confidence Interval to tell us how accurate our mean is. the difference (z) between the distribution of sample means and the distribution of population means is very small. . The equation for standard error is as follows: SE = SD of sample means divided by square root of n Example #2: Remember: +/.“n” is the sample size. take the sample mean (100) and subtract 2 SE (2 x 1) → 98 3. March 15.” A 95% CI = sample mean +/.2 SE includes 95% of the area. we sampled systolic bp. and the sample size is 100 people. Confidence Intervals In the above example. between the population mean and the sample mean.2 SE At this point we watched a video about confidence intervals that reiterated what was covered in this lecture and then moved onto the workshop to practice our newly acquired statistical skillz. Calculate SE (SD of sample means divided by square root of n): . we are saying that we are 95% sure that the real systolic bp is between 98 and 102 mmHg.