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UNIVERSITY OF PUNE

B. Tech. Biotechnology Third Year Syllabus (2008)

Structure for T. E. Biotechnology (2008) TERM – I Subject Code No. 315461 Teaching Scheme Subject Theory Genetics Engineering 3 4 4 4 3 18 10 Practical 4 2 2 2 TW/ Drawing Examination Scheme Paper 100 100 100 100 100 500 50 25 175 25 Practical 50 Oral TW 25 50 50 50

Total
marks

175 150 150 175 100 750

315462 Mass Transfer 315463 Heat Transfer Fermentation Technology I Computational 315465 Techniques and Biostatistics 315464

TERM – II Subject Code No. Teaching Scheme Subject Theory 4 4 3 3 4 2 2 2 2 18 10 500 50 75 Practical 2 TW/ Drawing Examination Scheme Paper 100 100 100 100 100 50 50 25 25 50 125 Practical Oral TW 50

Total marks

Reaction 315466 Engineering 315467 Fermentation Technology II

150 100 150 150 150 50 750

315468 Bioseparation I Immunology and Diagnostics Bioinformatics & 315470 Management 315469 315471 Seminar

E.T. BIOTECHNOLOGY SEMESTER I .

Enzymes used in GE: DNA modifying enzymes. Expression Vectors. restriction enzymes. PCR-design and optimization. transgenic plants and their applications UNIT 6 [8 Hrs] Applications of rDNA technology in health and agriculture: Humulin. Human genome project. DNA markers for improvement of quality and yield of crops. . cosmids. isolation. genomic DNA libraries. vectors for cloning in eukaryotic cells. Multiple cloning sites. sequencing. lambda phage. DNA ligase. Southern Blotting. sequencing strategies and analysis and applications.GENETIC ENGINEERING (315461) Teaching scheme: Theory: 3 hr/week Practical: 4 hr/week Exam Scheme: Paper: 100 Marks Practical: 50 Marks Term Work: 25 Marks --------------------------------------------------------------------------------------------------------------------- UNIT 1 [8 Hrs] Techniques and tools in genetic engineering. AFLP. selection markers. Application of transgenic animals. Cloning strategies: DNA cloning. factorVIII. Conjugation. DNA diagnostics. Gene therapy. BT cotton. artificial chromosomes (BACs. YACs) UNIT 3 [8 Hrs] Gene libraries. Translocation. Plasmids. RTPCR. Golden rice etc. Transformation. modifying enzymes. cDNA library. cDNA synthesis. transgenesis and cloning. Hep B. and synthesis of gene. selection of recombinants UNIT 4 [8 Hrs] Screening libraries. artificial DNA synthesis. polymerase for GE UNIT 2 [8 Hrs] Cloning vectors. RAPD. Modification of bacteria and viruses: live vaccines. identifying the products of cDNA clones. amplification of gene libraries. RFLP. Automated DNA sequencing method. Animal transgenesis. micro arrays. techniques of DNA sequencing. phgemids. M13 vectors. Transfection. different methods of gene isolation. PCR cloning UNIT 5 [8 Hrs] Gene transfer technologies. flow cytometry. Screening of recombinant clones.

From genes to genomes: concepts and applications of DNA technology by J. W. Isolation of template DNA Isolation of vector DNA RE digestion of vector and template Ligation with plasmid DNA Transformation Selection of recombinants Conformation of insert PCR Real time PCR (demo) Text Books: 1. Dale and M.Schantz (Wiely Publishers. 6.V. 3.Practicals: 1. PANIMA Gene cloning and DNA Analysis: An introduction (4th edition) by T. 9. Brown Molecular cloning by Sambrook. Principles of Gene manipulation and Genomics by Primrose and Twyanman (Blackwell Publishers) 2. 4. 2. et al . 2. 8. Molecular biotechnology by Pasternack and Glick From Genes to clones by Winnacker. 4. 3. 7.) Reference Books: 1. A. 5.

Methods of conducting mass transfer operations. NTU. Low pressure. Tray efficiencies. Rate of drying curve. Mechanism of gas absorption. Molecular Diffusion in gases and liquids. Multi component system. Batch drying. Equilibrium. Introduction to Inter phase mass transfer. Multistage tray towers-McCabe Thiele method. Use of Reflux. L/G ratios for absorbers. absorption with chemical reaction. Vapor-liquid equilibria for Ideal and Non-ideal systems. Azeotropes. Numericals relating counter current operations UNIT 5 [9 Hrs] Drying. Numericals relating drying operations . Drying operations. Two resistance theory. Classification of drying equipments.MASS TRANSFER (315462) Teaching Scheme: Exam Scheme: Theory: 4 hr/week Paper: 100 Marks Practical: 2 hr/week Term Work: 50 Marks -----------------------------------------------------------------------------------------------------------UNIT 1 [10 Hrs] Introduction. Steam distillation. Minimum reflux ratio. Differential. coefficients. Diffusion in Solids. Positive and negative deviations from Ideality. partial. Batch rectification. Optimum reflux ratio. Cascades. types of solid diffusion. Heat and Momentum transfer analogies. General principles of Mass Transfer. Flash. Condensers. Local and overall mass transfer coefficients. Relative volatility. Reflux ratio-Total reflux. Design principles Diffusion and Mass transfer. Fick’s and Maxwell law of diffusion. Types of moisture binding. Real trays and Tray efficiency. UNIT 2 [8 Hrs] Distillation Definition. Mechanism of moisture movement in solid continuous drying. Classification of Mass Transfer Operations.Molecular diffusion. Absorption factor. Methods of distillation-Continuous rectification. Turbulent diffusion. Non ideal liquid solutions. Ideal solutions-Raoult’s law. Choice of solvent for absorption. Drying hysterisis. Azeotropic. HTU. Types of diffusion . Types of condensers-Total condensers. Mechanism of batch drying. Ideal liquid solutions. Use of local overall. Definition. Equilibrium in drying. Mass. Stages. Extractive. Choice of separation method. Types of reboilers. Diffusivities of gases and liquids. Material balances – one component transferred in counter current flow. HETP concept and calculations UNIT 4 [9 Hrs] Gas Absorption. Rate of batch drying. Time required for drying. Principles. Theories of Mass transfer. Equilibrium in gas absorption. Molecular distillation UNIT 3 [9 Hrs] Tray tower calculations Continuous rectification for binary system. Fenske’s equation. Numericals relating various types of diffusion.

and |Richardson J. McGraw Hill Reference Books: 1. Principles of Unit Operations in Chemical Engineering. Batch/continuous leaching 8.. Liquid liquid Diffusion – To calculate the Diffusion Coefficient for a liquid – liquid system 2.C. Enthalpy balances.D. and Smith J. Simple Distillation 5.L. McCabe W. Multistage crosscurrent liquid .E.M. evaluation of term work should be done through periodic tests • Record of assessment of practical should be maintained on continuous basis Textbooks: 1. Vol I & II – Coulson J. 3. Fluidized Bed Dryer – To study the characteristics of Fluidized bed Dryer 11. Principle rate of crystal growth..F. Steam Distillation 6. Chemical Engineering.UNIT 6 [8Hrs] Crystallization . Solid liquid diffusion – To calculate the Diffusion Coefficient for a solid –liquid system 3. Process of Crystallization and its Characteristics 4. Wetted Wall Column – To find the mass transfer coefficient in a wetted wall Column 12. Design of Equilibrium Stage Processes .Smith B. Tray Dryer – To study the characteristics of Tray Dryer 9. Mass Transfer Operations – Treybal R. Unit Operations of Chemical Engineering. McGraw Hill 2. Interphase Mass Transfer Coefficient – To calculate the individual and overall Mass Transfer Coefficient 10.S. Calculations of yield. Spray Dryer – To study the design and Operating Principles of Spray Dryer Note on Term Work: • Minimum number of experiments – 8 • Minimum number of assignments – 2 ( out of remaining experiments) • Besides experimental work. . Population balance and size distribution. Equipment Practicals: 1. McGraw Hill 2. Foust A. .liquid extraction 7.

the shape factor. Concept of maximum heat flux and critical temperature drop. Kirchoffs law. properties of insulator. various cases of radiation between two surfaces. Introduction to unsteady state condition.HEAT TRANFER (315463) Teaching Scheme: Exam Scheme: Theory: 4 hr/week Paper: 100 Marks Practical: 2 hr/week Term Work: 50 Marks -----------------------------------------------------------------------------------------------------------UNIT 1 [8Hrs] Introduction: Modes of heat transfer. Heat transfer in boiling liquids: Pool boiling of saturated liquid. LMTD in parallel and counter flow. infinitely long hollow cylinder and hollow spheres. Basic equation of heat transfer by radiation. through annulus. Planks law. UNIT 4 [8 Hrs] Radiation: Fundamental facts and definition of terms: Emissivity absorptivity. Natural and forced convection in laminar and turbulent flow. thermal conductivity of liquid. fouling factor. Importance of dimensional analysis in experimental design and data reduction. over the plate. Principal and heat balance equation in laminar flow and empirical equations for turbulent flow through tube. gases and solids. gray body. conduction. Concept of thermal resistance. UNIT 2 [8 Hrs] Conduction: Fourier’s law of heat conduction. Differential equation from shell balance for unsteady and steady state conduction. Significance of dimensional analysis in heat transfer. thermal insulation and optimum thickness of insulation. Condensation on vertical plate and horizontal plate. UNIT 5 [8 Hrs] Heat exchange equipment: Types of heat exchangers including compact heat exchangers. Heat losses through pipe. units of various quantities used in heat transfer dimensional analysis. and radiation. opaque body. . black body. UNIT 3 [8 Hrs] Convection: Newton’s law of cooling. Heat transfer with phase changes: Condensation: Modes and features: Theory and derivation of Nusselt’s equation. individual and overall heat transfer coefficient. convection. Wien’s law. parallel flow arrangement. Thermal resistance in composite slab and cylinder. Concept of thermal boundary layer and its significance. classification of extended surfaces. Effectiveness NTU method. Stefan Boatmen law. thermal conductance and contact resistance. Steady state condition in infinitely long slab. efficiency of longitudinal fin. Heat transfer from extended surfaces with uniform cross section.

Boiling point elevation. 11th ed.N.E. Material balance calculations. “Coulson Richardson’s chemical engineering vol. Open pan evaporator 10. Heat transfer in agitated vessels 8. Wiley Publications.. Universities Press (India). Kern. capacity and economy. Stewart W. “Transport phenomena” 2ed. “Principles of Heat Transfer” 5th edition. New Delhi 3. heat transfer coefficients. Multiple effect evaporators: Methods of feeding. “Process Heat Transfer”. Thermal radiation-determination of emissivity 4. New Delhi (2004) 2. “A Textbook on Heat Transfer”. Tata McGraw Hill Publications. D. performance. 4th ed. Lightfoot E. Heat pipe demonstrator 11. S. Double effect evaporator 9.B. Sukhatme. Bird R. Frank Kreith. 1993 . Plate Heat exchanger 7. capacity and economy.K. Shell and tube heat exchanger 6. Double pipe heat exchanger 5. J P Holman. “Heat Transfer” 9th edition.6” pergamonpress.2002 Sinnout R. 2005 Reference Books: 1. P. Heat conduction 2.UNIT 6 [8 Hrs] Evaporation: Types of evaporators. Fluidized bed heat transfer Text Books: 1. Mark Bohn. PWS Publishing company.. Natural convection 3. effect of liquid head and boiling point elevation Practicals: 1... Tata Mc Graw Hill Publication. Q. Boston (1997) 2.

FERMENTATION TECHNOLOGY I (315464) Teaching Scheme: Theory: 4 hr/week Practicals: 2 hr/week Exam Scheme: Paper: 100 Marks Orals: 25 Marks Term Work: 50 Marks -----------------------------------------------------------------------------------------------------------UNIT 1 [8 Hrs] Introduction to Microbial Fermentation. Modes of operation in stirred reactors: discontinuous batch operation. different types of sterilization techniques – their mechanism of destruction. packed bed. optimization for maximum yield Sterilization: Need for sterilization.Screening and isolation of microbes. Microbial production of vitamins B2 and B12 Microbial Enzymes: Production. sources of nutrients i. Citric. Antifungal antibiotics. applications Types of Fermenters.case study. hollow fiber etc. semisynthetic penicillins. Concept of upstream processing . isolation and use of microbial enzymes. Advantages and Disadvantages. beer UNIT 4 [8 Hrs] Production of Antibiotics and Microbial enzymes.e. and periodic fed-batch cultivation Solid State fermenters: Tray bioreactors. chloromycetin. effect of media components on fermentation. nitrogen etc. Animal & Plant Cell Reactor Technology. glycerol. gluconic. Bubble Column. Preserving industrially important microbes. Different types of media. semi-continuous reactors.Types and applications UNIT 5 [8 Hrs] Different types of fermentation: Submerged and solid state fermentation. Inoculum preparation. continuous operation. advantages and disadvantages. HTST UNIT 3 [8 Hrs] Microbial production of industrially important products: Alcohols and acids. Microbial fermentation of organic acids viz. Configuration and applications. tetracyclines. streptomycin. Advanced Fermenter designs: Microfermenters for Rapid screening and analysis of Biochemical processes. Submerged fermenters: Stirred tank. fumeric. itaconic. Air lift. Monod growth kinetics UNIT 2 [8 Hrs] Media Preparation and optimization. gibberellic and kojic acids. Hollow fiber etc. microbial / Industrial fermentation: Applications for production of industrially important products. media preparation. Activities of acetic acid bacteria and production of vinegar Alcohol Production: wine and other alcoholic beverages.Therapeutic Antibody Production . nutrient supply. Disposable Fermenters:. Antibacterial antibiotics: penicillin. Immobilization . carbon.. Examples of classical fermentation systems. in situ sterilization. Activities of lactic acid bacteria and industrial production of lactic acid..

theoretical considerations & techniques used. Bioprocess Engineering Principles by Paulin M. Nitrogen etc.4: M. Calculation of fermenter efficiency and cost of product 11. Kinetics of cell growth 4. Fermentation of any two microbial products 6.D. Stanbury. Humphrey. 4. Kinetics of product formation 5. 5.) on fermentation Text books 1. product recovery. 1.UNIT 6 [8 Hrs] Fermentation Scale up and economics: Scale-up: Principles. Brock. Sterilization. Whitaker.) Reference Books 1. London) 6. Effect of aeration in fermentation 9. Pergamon Press. Effect of different media components (Carbon. Hall. Second Edition.Y. Biotechnology: A Text Book of Industrial Microbiology: T. 7. 1990. Smaeur Associates.E and Milli N.. Effect of temperature/ pH in fermentation 8. Study of fermenter design 3. Solid state Fermentation 10. S. Industrial microbiology: Casida Newage Publication 2001 2. product purity. 2001 Industrial microbiology: Prescott and Dunn CBS publications 4th Edition. 3. Use of alginate for cell immobilization. 1999 Comprehensive Biotechnology Vol. New York. Biochemical Engineering Fundamentals by Bailey and Ollis (McGraw Hill. Principles of Fermentation Technology. Elsevier publication 3.Doran ( Academic Press. 2. Fermentation and product recovery costs. Isolation and screening of industrially important microorganisms. operation parameters Concept of downstream processing. 2. case example such as ethanol economics Introduction to GMPs Practicals (Any 8): 1.F . Young (Eds. yields. Enzymes: Trevor. A. Horwood. fermentation efficiency. Biochemical Engineering by Aiba S.). inoculum development.

Factorial notation. and Mean deviation. divided differences UNIT 3 Numerical Integration: Newton Cote’s quadratic formulae. Standard deviation. continuous series. Finite differences and Interpolation: Forward differences. fitting of other curves. Solutions of Non linear simultaneous equations: Newton Raphson method for non linear equations. Tests of Significance: Students t . individual observations.distribution (parametric). Regular false method Solutions of linear simultaneous equations: Newton Raphson method. Geometric mean. . UNIT V [8 Hrs] Biostatistics: Introduction to Biostatistics Sampling: Introduction. Harmonic mean for discrete series. central differences. Types of averages. fitting of a parabola using polynomial regression. pie chart etc). deductions from NR method. Sample method. Calculation of Arithmetic mean. General rules for constructing diagrams. Sample size. Mode. Simpson’s 3/8th rule. sampling errors. cluster sampling.COMPUTATIONAL TECHNIQUES AND BIOSTATISTICS (315465) Teaching Scheme: Exam Scheme: Theory: 3 hrs/week Paper: 100 Marks -----------------------------------------------------------------------------------------------------------UNIT 1 [6 Hrs] Linear and Polynomial Regression: Method of Least Squares: Fitting of a straight line using linear regression. theoretical basis of sampling. Median. UNIT VI [8 Hrs] Types of averages: Introduction. Trapezoidal rule. Chi square test (non parametric). Frequency distribution graphs. backward differences. Interpolation with unequal intervals: Lagrange’s formula.determination of sample size. Significance of graphs and diagrams. random and systematic sampling. UNIT 2 [10 Hrs] Interpolation. Simpson’s 1/3rd rule. Essentials of Sampling. Types of diagrams (Bar. Weddle’s rule [6 Hrs] UNIT 4 [10 Hrs] Solutions of Algebraic equations: Bisection method. Newton’s Interpolation formulae with equal intervals: Newton’s forward and Newton’s backward formulae. Diagrammatic and graphic presentation: Introduction.

Text books: 1. S. Steven C Chapra.Iyengar. B. M. Numerical methods for scientific and engineering computation.K. Dr. Reference books: 1.K. R.R. New Delhi. 5th edition.S.Gupta . 3. Statistical methods.Jain. Khanna Publishers. 2.Grewal. Numerical methods for Engineers with software and programming applications.P.Sultan chand and Sons Educational Publishers.K. S. New Age International Publishers. Reynolds P Canale. .Jain. Higher Engineering mathematics.

BIOTECHNOLOGY SEMESTER II .T.E.

fractional yields.molecularity and order of reactions. surface kinetics. . enzyme deactivation kinetics Microbial growth kinetics: Product distribution. fluid particle reactions – progressive conversion model. Kinetic implications of endogenous and Maintenance metabolism.single and multiple reactions. the concept of rate controlling step. fluidized bed reactors. conversion in a non-ideal system. Definition and significance of rate of reaction .searching for a reaction mechanism. Monod growth kinetics. RTD studies. performance equations. dispersion model. environmental effects on growth kinetics. plug flow reactor – concept of space time and space velocity. elementary and non elementary reactions. Definition of non-ideality. pore diffusion. comparison. Thiele’s modulus. performance of different reactors and their comparison. conversion. substrate and product limiting microbial fermentation. Introduction – examples of heterogeneous systems: solidliquid systems. Mixed flow reactor. Introduction: Classification of reactions . Models for non-ideal reactions. tanks in series model segregated flow model. Analysis of batch reactor data.REACTION ENGINEERING (315466) Teaching Scheme: Exam Scheme: Theory: 4 hr/week Paper: 100 Marks Practical: 2 hr/week Term Work: 50 Marks -----------------------------------------------------------------------------------------------------------UNIT 1 [8 Hrs] Fundamentals of Reaction Engineering. Mixed flow reactors. Distribution curves – the E. Arrhenius and Collision theory . catalysis. recycle reactor UNIT 6 [8 Hrs] Application of reaction engineering to biochemical reactions Michaelis Menten kinetics. F and C curves. factors affecting rate of reaction – Temperature dependency. packed bed catalytic reactor. concept of rate controlling step UNIT 2 [8 Hrs] Introduction to Reactor Design. shrinking core model. Different types of reactors: Batch (Constant and variable volume). age distribution. performance equation for porous catalysts. enzyme inhibition kinetics – competitive and noncompetitive. UNIT 4 [8 Hrs] Heterogeneous Reactions. determination of rate controlling step UNIT 5 [8 Hrs] Heterogeneous Systems. their significance and the relation between them. activation energy. effectiveness factor. kinetic models for non-elementary reactions . problems on performance and conversion UNIT 3 [8 Hrs] Non-ideal Reactor Systems. slurry and trickle bed reactors.

Non ideal reactions-F & C curves in PFR 9. Chemical Engineering Kinetics: Smith J. 2. Elements of Chemical Reaction Engineering: H. Evaluation of PFR followed by CSTR 10. Reactor design and analysis: Bishoff and Fromment. 4. Reaction kinetics of first order reaction. Design of two PFR in series Textbooks 1. Fogler. Academic Press Reference Books 1. John Wiley Publishers. Reaction kinetics of pseudo first order reaction-Acid catalyzed hydrolysis of methyl acetate 3. Design of PFR 5. Design of CSTR combination in first order reactions 7. Non ideal reactions-F & C curves in CSTR 8. Design of CSTR 6. 2. Oxford University Press . Mc Graw Hill Publication 2.Practicals (Any 8): 1. Reaction kinetics of a second order reaction – Saponification of ethyl acetate.. Chemical Reaction Engineering: Levenspiel O. Scott.

centrifuges. Criteria of membrane separation processes. Adsorption Isotherms . and Temperature Swing Adsorption (TSA). effect of rheological properties and its importance in fermentation operation Aeration and agitation – impeller design. Parameters affecting fermenter performance: pH. Definition of a membrane. dissolved oxygen. Membrane modules and Industrial applications of all processes .FERMENTATION TECHNOLOGY II (315467) Teaching Scheme: Exam Scheme: Theory: 4 hr/ week Paper: 100 Marks -----------------------------------------------------------------------------------------------------------UNIT 1 [9 Hrs] Fermenter design and operation. Nature of adsorbents. Factors effecting leaching operations. Single stage leaching. advantages and disadvantages of various equipments . tray towers. spray dryers. thickeners. trickle bed reactors. applications. effect of shear UNIT 2 [9 Hrs] Equipments for processing fermentation products. agitator power requirement. nutrient supply. temperature. Methods of operation. continuous countercurrent decanters. rotary filters UNIT 3 [9 Hrs] Adsorption and Ion Exchange Definition. membrane contactors. Pervaporation.Micro filtration. drum filters. effect of aeration and agitation in mass transfer. Piezodialysis. Carrier mediated transport. mass transfer theory. oxygen requirement of microorganisms. Techniques and applications. BET. freeze dryers. Heat of adsorption. Industrial Applications. Types of membranes.Langmuir. Introduction to Pressure Swing Adsorption (PSA). diffusional resistance to oxygen transfer.liquid membranes. Equipments for adsorption Principles of Ion Exchange. foam. Membrane fouling. packed towers. Configuration. Freundlich.Physical and Chemical. Membrane separations . vacuum dryers. Reverse Osmosis. Continuous counter current leaching. Membrane contactors. Advantages of membrane separation processes over conventional separation techniques. Calculation of single stage and multistage leaching processes UNIT 5 [9 Hrs] Membrane Separation Techniques : Classification of separation techniques.bubble columns. Leaching equipments. agitated vessels. Constant and variable underflow. measurement of mass transfer coefficient and factors effecting them. Ultra filtration. Equilibria and rate of ion exchange UNIT 4 [9 Hrs] Solid – Liquid Extraction (Leaching): Definition. Electro dialysis. Preparation of the solid. Need of different unit operations for processing fermentation broths and products. Polarization phenomenon. Types of Adsorption . Membrane electrolysis.

“ Design of Equilibrium Stage Process”. 4. Hall. Material balances . 1996. Multistage crosscurrent. “ Chemical Engineering”. “ Principles of Unit Operations” 2. 2004 . Foust A. Ternary liquid equilibria. Fields of usefulness. J. F. Bioprocess Engineering Principles by: Pauline Doran Publisher: Academic Press 1. countercurrent and co current extraction.D. Types of extractors – stage type and differential type Text Books: Treybal R.UNIT 6 [9 Hrs] Liquid-Liquid Extraction (Solvent extraction) : Definition. Second Edition (9780750645010): PF Stanbury. McGraw Hill 3. S. 2nd Ed. II Smith B. and Seader H. 3. “Mass Transfer Operation” Richardson J.E. Richard Baker. “ Unit Operations in Chemical Engineering” 4. King C. McCabe and Smith.S.M. Basic Principles of Membrane Technology.. “Stage wise Process Design” . Henley E. “ Separation Processes”. I . Equilateral triangular coordinates.. A. Principles of Fermentation Technology. Wiley. Reference Books 1. Marcel Mulder. Mixture rule. McGraw Hill 5. J. Whitaker 5. Vol.K. Kluver. 2. Membrane Technology and Applications.Single stage extraction.. and Coulson J. Choice of solvent.

.Process Economics. General description of column chromatography. reverse osmosis UNIT 4 [8 Hrs] Leaching. Chromatographic terms and parameters. HPLC. crystallization. Classification of chromatographic techniques. planar chromatographic techniques. Clarification Techniques: Sedimentation 3. centrifugation. Aqueous two phase extraction. Types of chromatography UNIT 3 [8 Hrs] Basic separation techniques: sedimentation. ultracentrifugation.Process analysis. its scope from research to industry. adsorption UNIT 2 [8 Hrs] Chromatography –Separation Technique. Illustrative Examples UNIT 6 [8 Hrs] Industrial applications with examples. filtration. Mechanical and enzymatic methods of cell disruption. Use of sonicator for cell disruption 9. homogenization.BIOSEPARATIONS I (315468) Teaching scheme: Exam scheme: Theory: 3 Hrs / week Paper: 100 Marks Practicals: 2 Hrs / week Practical: 50 Marks -----------------------------------------------------------------------------------------------------------UNIT 1 [8 Hrs] An overview of Bioseparations. Steroids and antibiotics Practicals (Any 8) 1. 5. lyophilzation. To use flocculation as a unit operation 10. Synthesis of Bioseparation Processes. use of solvent extraction in antibiotic separation. Precipitation of proteins using Ammonium Sulphate. scale up of chromatography. use of membranes (semi permeable) in purification. chromatography column dynamics. affinity extraction/ chromatography UNIT 5 [8 Hrs] Importance of separation techniques in biotechnology. Chemistry of extraction. Adsorption on charcoal: Application in removal of unwanted dye. ultrasonication. drying. process consideration in preparative chromatography. Practice of chromatography. gradient centrifugation. absorption. Separation of bioconversion products/ secondary metabolites e. 2. Lab scale Homogenization of Baker’s Yeast 4. extraction. micro/ultra filtration. selection of solvent. 7. Dialysis of proteins followed by concentration of proteins with dialysis bag. Chromatography for separation of biomolecules 8. importance of cell disruption in product release.g. Use of blender for disruption of plant tissues 6.

Wiley.L.S. E. Bioseparation: Downstream processing for Biotechnology. P. McCabe. Belter. J. W. Hu. Biosepration Engineering: Principles. practice and economics. Unit Operation of Chemical Engineering. McGraw Hill.J. Seader. 2. Interscience Reference Books 1.D. Separation Process Principles.. New York.. 2.L.Textbooks 1. . E. W. P. and Henley. Wiley.A.. Smith. Wiley. and Harritt. and Cussler.C. J.

Western blotting UNIT 3 [8 Hrs] Cell mediated immunity. role of adjuvants. theories of antibody production. Monoclonal Antibody. Major Histocompatibility Complex (MHC). Physiological barriers against infection. cytotoxic T cells.principles and applications. c) Blocking. reaginic antibody.clonal selection theory. complement system. TB. immunoglobulins. d)Hybridization. precipitation. ELISA. cell mediated effector responses. b) Blotting. UNIT 2 [8 Hrs] Antigens. Hybridoma Technology. active and passive immunization. Demonstration of Western Blotting: a) SDS PAGE.IMMUNOLOGY AND DIAGNOSTICS (315469) Teaching Scheme: Exam Scheme: Theory: 3 hr/week Paper: 100 Marks Practical: 2 hr/week Orals: 50 Marks -----------------------------------------------------------------------------------------------------------UNIT 1 [8 Hrs] Overview of immune system. innate and adaptive immunity. Natural killer cells. 2. c) washing. Peripheral blood smear Quantitation detection of immunoglbulin using precipitation reaction Haem-agglutination Determination of antibody titer by Radial Immuno Diffusion (RID) Ouchterlony Immunodiffusion ODD ELISA: a) Coating. attenuated vaccines. antigen processing and presentation. T cells UNIT 4 [8 Hrs] Immune effector mechanisms. antigen antibody interactions. ADCC. 7. Structure and function of antibody. phagocytosis. autoimmunity. Immunodeficiency. 3. Type I-IV reactions UNIT 5 [8 Hrs] Vaccines. organization and expression of Ig genes. antisera. Cells and organs of immune system. inflammation. RIA. GVH. cancer and immune system. b) Blocking. 5. 6. generation of antibody diversity. recombinant vaccines. agglutination. graft rejection. Activation of B cells. DNA vaccines UNIT 6 [8 Hrs] Immune system in diseases. subunit vaccines. 4. hypersentitive reactionsallergen. humoral immunity. cross reactivity. d) Ag-Ab reaction. e) HRP Detection 8. cytokines. toxoids. e) Detection . AIDS Practicals: 1.

Cold spring harbor laboratory.Text Books 1. Kindt. . Oxford.Blakswell Scientific Publications. Kuby. Goldsby.) Raven Press. 2. Antibodies A laboratory Manual: Harlow and David Lane (1988).E.A. Fundamentals of Immunology: Paul W. Osborne. B.Immunology (4th Edition) by R.J. 1988. (Eds. New York. A. 1988 Reference Books 1. T. Essentials of Immunology (6th Edition): Ivan Roit. 2.

Unit 4 (8 Hrs. Calculation of alignment's statistical significance. Drug designing – Ligand based and Structure based. Working of FASTA and Variants. Unit 3 (8 Hrs) Protein structures. CATH. Scope and Goal of Bioinformatics. Substitution Matrices such as PAM And BLOSUM. Phylogenetic analysis. Terminologies used in Bioinformatics. UniGene. General introduction to management studies. MIPS. Sequence retrieval systems such as Entrez and SRS. SWISS-PROT. Homologes. Specialized genomic recourses as SGD. Object oriented and Relational databases. Importance of Identity matrixes. Primary protein sequence Databases as PIR. HTS. Pairwise and Multiple Sequence Alignment. Object Model. Nucleotide databases as Genbank. Network of databases for Entrez and SRS. Various analytical tools for protein Structure Visualization. PRINTS.Bioinformatics & Management (315462) Exam Scheme: Paper: 100 Marks Oral: 25 Marks Term Work: 25 Marks ----------------------------------------------------------------------------------------------------Unit 1 (8 Hrs. Unit2 (8 Hrs) DNA sequencing. Smith Waterman Algorithm.) Introduction to Bioinformatics. Application of bioinformatics in vaccine designs. Correlation of the management and biotechnology industry. Databases. QSAR. Unit 5 (8 Hrs) Introduction to Phylogeny. Overview of applications of Bioinformatics. TrEMBL. orthologs and paralogs. Teaching Scheme: Theory: 4 hrs/week Programming: 2 hrs/week Unit 6 (8 Hrs) Introduction to biotechnology Management. Methods of Phylogenetic analysis as Distance method. Technology transfer management in Biotechnology .Relational Model. TDB. gaps and penalties. Construction of a phylogenetic tree. Overview of Biological Databases. BLAST.) Introduction to sequence alignment. Heuristic methods such as FASTA. Data structure. Dot Plot. Database query language. Database Management system. Levels of Protein sequence and Structural organization. OWL. NCBI’s Data model. Needleman Wunsch Algorithm. Dna Data Bank of Japan ( DDBJ). scoring function for Docking. Protein databases. Working of BLAST and different variants of BLAST. Different types of trees as rooted and unrotted trees. Composite Protein sequence databases as NRDB. European Molecular Biology Laboratory (EMBL). various disciplines in management. Blocks. Organizational management in biotechnology companies. Structure Classification databases as SCOP. Secondary databases as PROSITE. Local and Global Sequnce Alignment. PDB.

F. 2. Mandiratta. Database searches: Search and retrieval of sequence data from Nucleotide databases as Genbank. Baxevanis and B. Lesk Oxford University Press.g. Oxford University Press. Introduction to the NCBI: Use of different features and services enlisted in NCBI. Introduction to Bioinformatics. P.Methods & Applications by S. .F. 4. BLAST and FASTA: Use of BLAST and FASTA programs. 8.Practicals: 1. DDBJ. 3. tertiary structures etc 5. Bioinformatics: A practical guide to the analysis of genes and proteins A. 2002 John Wiley and Sons. PFAM.Rastogi.C.Knoots and O. Cold Spring Harbor Laboratory Press.D. Pair-wise Sequence Alignments and their interpretation/analysis: sequence alignments using implementations of Needleman-Wunsch and Smith-Waterman algorithms 7. N. Multiple Sequence Alignments using CLUSTAL-W method with analysis of the alignments to find conserved. 2. Bryan Bergeron. variable regions. Bioinformatics. 3. “Bioinformatics computing”. 2. EMBL.Donnell Reference Books 1. Ouellette (Eds). 2nd edition. SwissPDBViewer to visualize protein structures and understand secondary structural motifs. Rastogi. Structure Visualization and Analysis: Use of different visualization software such as RasMol. 3. Pearson Education [BB]. by Arthur M. Sequence and Structure Classification: Search and retrieval of data from sequence and structure classification data bases with analysis of search results (e. Bioinformatics Sequence and Genome Analysis by David W. Database search Engines: Search and retrieval of sequence and structure data using query engines such as Entrez and SRS. Mount. Principles of management. SCOP databases) Text Books 1. interpretation of results to identify homologues of genes/proteins from databases 6.