The Treatment (V1)

For this commission the animation will concentrate on the making of an antibody. The
animation will be divided into two portions: Primary and secondary attack. In the
first attack, the animation will show the arrival and contact with the antigen,
causing a slow and meagre secretion of antibodies to be reproduced (anticipation
phase). The second portion will show the same antigen; this time a secondary response,
faster and more significant, which confirms the existence of immune memory. This is
explained by the formation of memory b-cells and T4-lymphocytes bearing specific
receptors for this antigen. The second process is important because the T4-lymphocytes
differentiate into CD4-cells secreting chemical messengers called interleukins. The
magnitude of the secondary response will show that the number of memory cells specific
for the antigen is more important than the initial attack. The clonal proliferation
induced by the first contact with the antigen will generate long-lived "butterfly-
esque" memory cells (as part of my artistic license and art direction)/a resolved
animation.

The bodies humoral, or antibody-mediated immune response begins in the same manner as
its cell-mediated response. The macrophages are joined by lymphocytes called "b-
cells". The pathogen activates only those b-cells with matching receptors - these
cells will stand ready to enter the battle against unwanted invaders. Meanwhile, the
antigen presenting macrophages activate those helper t-cells (the pathogen
killers/"cytotoxic") with matching receptors, these in turn rendezvous with activated
b-cells. Triggered by this meeting, the helpers release chemicals which spur the
selected b-cells into rapid reproduction. This allows for cell proliferation which is
a clonal proliferation.

Some b-cells become memory cells ready to respond to a later invasion by the same
pathogen, but most become antibody producing factories called plasma cells. At the
same time, differentiation takes place: the stimulation of the bcl2-gene that protects
against cell death and plasma cells that secrete specific antibodies to the antigen
inducing the response.

Patrolling the body, antibodies dock with pathogens; this neutralises them, or marks
them for destruction by other weapons in our immune arsenal. Humoral Immune Response
(HIR) is initiated by a macrophage, which takes up the pathogen (virus) and processes
it. The binding from a immunoglobulin leads to the formation of an immune complex. It
promotes the involvement of innate mechanisms of elimination: the end of the constant
parts of the antibody molecule can bind to receptors on the membrane of phagocytic
cells, macrophages and granulocytes. These cells then ingest the immune complex by
phagocytosis and then eliminate it.

The Step Outline/Script in making (V1)

• FADE IN
• Camera will rush through the blood vessel while opening credits play out
• On screen text will blend in with the animation throughout...
• The sound FX of body pulsation and blood vessel current/general current (with
special CG FX where appropriate)
• CROSSFADE TO
• Continued cut of Blood vessel
• CROSSFADE TO
• In the cellular response involving T lymphocytes, the humoral response is the
production of antibodies
• Zone in on a b-lymphocyte (white blood cell (comes from bone marrow))
• Bone marrow is the organ that produces most of immunoglobulin.
• The b-lymphocyte contains many proteins on its surface:
o Membrane bound antibodies/Immunoglobulins (which will look like
anemone/genus/"cute" caterpillar-esque)

Unit 6 - Commission
CG Arts & Animation Yr 1
Dayle Sanders
 • The b-cell is moving gracefully and the camera is following (the unique process
begins)
• This is important inasmuch that the membrane bound antibodies (two types of
antibody variations: the first is surface bound) from one b-cell to another b-
cell can take on a bunch of different forms
o Different variable portions (nibs or parts of the antigen invaders)
o Over 10 billion variable portions
• ZOOM INTO
• DNA inside of the B lymphocyte nucleus. The DNA is not the same as other b-
cells, but they are reproduced with in a very similar clonal manner. There is a
lot of intentional reshuffling that occurs, which allows the immune system to
diversify itself
• This unique "diversification" means that the arrival of a bacteria/foreign
protein/antigen will only be compatible with a particular surface and its
epitope (part of the bacteria/said foreign protein) and will have to find a
suitable "bonding" combination:
• "The surface binding"/"Trial and error"/"Self" responding combination
o When the binding finally occurs, the B lymphocyte is "activated".
o Triggered binding will begin
• The B lymphocyte begins to multiply slowly into two different types of cells as
part of this trigger with special CG FX where appropriate):
o Memory cells: Higher quality defences are produced, containing the same
variable portions on them as the initial b-cell in question (these will look
butterfly-esque to signify the journey of growth (beauty of the process and
strength of the cell (of course they won't actually look like butterflies but
the cells themselves will contain a luminous wing like shape)
o Effecter cells: Reproductive and "active" cells: Known also as "Plasma
cells" (commonly) and "Antibody factories"
• CHANGE TO
• The pathogens will continue to infect the immune system, but the antibodies
will be going to continuously reproduce. They remember the pathogenic
interaction!
• They then stick (fly...) around in the fluids of the body until activated (when
the same pathogen infiltrates): The systematic arrangement for a better,
overall immune protection
• When activated, their colours and shapes metamorphosise into something
incredible
• After activation, the bonding is immediate (without hesitation/trial and error
stages because the memory cells know the pathogenic data, so trial and error is
not needed. They seek out pathogenic traces (such as the precise epitope(s)))
• The newborn antibodies (detached and floating in the fluid (the second type of
antibody which is not surface bound)) will begin the tagging process once they
locate the remaining fragments of the pathogen: This process is called
"opsonisation".
• The opsonisation is going to unique, obvious to the audience that something is
happening
• CROSSFADE TO
• This unique, immune signal, alerts one of the many macrophages. When one is
alerted, it promptly arrives on the scene to disable the pathogen:
o The macrophage on scene will prepare itself for phagocytosis (the
interaction consists of engulfing the memory cells and the pathogens,
imprisoning the pathogen for "interrogation" if you like)
• ...Keeps it prisoner (partially chunking it up and extracting information)
• Desired information: The macrophage wants to find out as much as possible about
the invader!
• The macrophage will present a piece of that consumed antibody on an "MHC2"
molecule (an antigen presenting cell)
• The MHC2 molecule moves towards the surface of the macrophage

Unit 6 - Commission
CG Arts & Animation Yr 1
Dayle Sanders
 • The specificity of this cell is shown by its features (it will glow slightly to
show the audience that it is important because it is essentially a rich cell
full of information to process)
• The animation ends coolly
• FADE OUT

The Premise (V1)

Two sequences will show how the human immune system develops antibodies to battle
against invaders, via primary and secondary attack.

The Logline (V1)

The humoral response occurs in two phases. The initial phase corresponds to the
recognition of antigens by B cells. The second involves the synthesis and secretion of
antibodies.

Unit 6 - Commission
CG Arts & Animation Yr 1
Dayle Sanders