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International Brokerage Event and Scientific Conference

"Sustainable Land Management and Vulnerable Ecosystems"

15-17 January 2003, Ljubljana, SLOVENIA

Delayed Fluorescence and Luminescence: A Versatile Tool for Risk Assessment and Monitoring in Sustainable Development

Alexis Zrimec 1 , Luka Drinovec 1 , and Maja Berden Zrimec 2

1 Institute of Physical Biology, Slovenia 2 Institute Bion, Slovenia

Keywords: light emission, stress detection, ecotoxicology, genotoxicity, nondestructive bioassays

The Problem

For better understanding and predicting the response of organisms and ecosystems to natural and human-induced disturbancesÖ

For better understandi ng and predicting the response of organisms and ecosystems to natural and human-induced

Ö it is necessary to develop nondestructive, objective, and quantitative, stress and quality indicators.

Ö it is necessary to develop nondestructive, objective, and quantitativ e, stress and quality indicators.

The methods used range from purely physico- chemical measurements to biological assays and ecological surveys

The methods used range from purely physico- chemical measurements to biological assays and ecological surveys
The methods used range from purely physico- chemical measurements to biological assays and ecological surveys

The methods used range from purely physico- chemical measurements to biological assays and ecological surveys

The methods used range from purely physico- chemical measurements to biological assays and ecological surveys
The methods used range from purely physico- chemical measurements to biological assays and ecological surveys
The methods used range from purely physico- chemical measurements to biological assays and ecological surveys
The methods used range from purely physico- chemical measurements to biological assays and ecological surveys
The methods used range from purely physico- chemical measurements to biological assays and ecological surveys

The methods used range from purely physico- chemical measurements to biological assays and ecological surveys

The methods used range from purely physico- chemical measurements to biological assays and ecological surveys

The methods used range from purely physico- chemical measurements to biological assays and ecological surveys

The methods used range from purely physico- chemical measurements to biological assays and ecological surveys
The methods used range from purely physico- chemical measurements to biological assays and ecological surveys

There is a need to develop methods which would share the sensitivity and quantitative nature of physico-chemical methods with the relevance of biological methods and at the same time be versatile enough to be used in many areas of environmental research

There is a need to develop methods which would share the sensitivity and quantitative nature of
There is a need to develop methods which would share the sensitivity and quantitative nature of

There is a need to develop methods which would share the sensitivity and quantitative nature of physico-chemical methods with the relevance of biological methods and at the same time be versatile enough to be used in many areas of environmental research

There is a need to develop methods which would share the sensitivity and quantitative nature of
There is a need to develop methods which would share the sensitivity and quantitative nature of

There is a need to develop methods which would share the sensitivity and quantitative nature of physico-chemical methods with the relevance of biological methods and at the same time be versatile enough to be used in many areas of environmental research

There is a need to develop methods which would share the sensitivity and quantitative nature of
There is a need to develop methods which would share the sensitivity and quantitative nature of

Possible Solution

Delayed Fluorescence and Luminescence

Delayed fluorescence is the long-lived light emission from previously illuminated biological material, whereas luminescence is spontaneous, nonstimulated, ultra-weak light emission from living cells (several orders of magnitude weaker than delayed fluorescence).

Delayed fluorescence is the long-lived light emission from previously illuminated biological material, whereas luminescence is spontaneous,

Delayed fluorescence is the long-lived light emission from previously illuminated biological material, whereas luminescence is spontaneous, nonstimulated, ultra-weak light emission from living cells (several orders of magnitude weaker than delayed fluorescence).

Delayed fluorescence is the long-lived light emission from previously illuminated biological material, whereas luminescence is spontaneous,

Delayed fluorescence of chlorophyll is correlated with chlorophyll content, photosynthetic activity, plant stress, crop quality, and taxonomic group

Delayed fluorescence of chlorophyll is correlated with chlorophyll content, photosynthetic activity, plant stress, crop quality, and

Delayed fluorescence of chlorophyll is correlated with chlorophyll content, photosynthetic activity, plant stress, crop quality, and taxonomic group

Delayed fluorescence of chlorophyll is correlated with chlorophyll content, photosynthetic activity, plant stress, crop quality, and

Delayed fluorescence of chlorophyll is correlated with chlorophyll content, photosynthetic activity, plant stress, crop quality, and taxonomic group

Delayed fluorescence of chlorophyll is correlated with chlorophyll content, photosynthetic activity, plant stress, crop quality, and
Delayed fluorescence of chlorophyll is correlated with chlorophyll content, photosynthetic activity, plant stress, crop quality, and

Delayed fluorescence of chlorophyll is correlated with chlorophyll content, photosynthetic activity, plant stress, crop quality, and taxonomic group

Delayed fluorescence of chlorophyll is correlated with chlorophyll content, photosynthetic activity, plant stress, crop quality, and

Delayed fluorescence can be used as an ecotoxicological stress indicator, a physiological indicator of plant viability, a monitoring tool for phytoplankton succession, density, composition and activity

Delayed fluorescence can be used as an ecotoxicological stress indicator, a physiological indicator of plant viability,

Delayed fluorescence can be used as an ecotoxicological stress indicator, a physiological indicator of plant viability, a monitoring tool for phytoplankton succession, density, composition and activity

Delayed fluorescence can be used as an ecotoxicological stress indicator, a physiological indicator of plant viability,
Delayed fluorescence can be used as an ecotoxicological stress indicator, a physiological indicator of plant viability,

Delayed fluorescence can be used as an ecotoxicological stress indicator, a physiological indicator of plant viability, a monitoring tool for phytoplankton succession, density, composition and activity

Delayed fluorescence can be used as an ecotoxicological stress indicator, a physiological indicator of plant viability,
Delayed fluorescence can be used as an ecotoxicological stress indicator, a physiological indicator of plant viability,
Delayed fluorescence can be used as an ecotoxicological stress indicator, a physiological indicator of plant viability,

Delayed fluorescence of DNA can be used as a genotoxicity test, because the fluorescence signal correlates with stress to nuclear DNA - which can be induced by genotoxic substances

Delayed fluorescence of DNA can be used as a genotoxicity test, because the fluorescence signal correlates

Luminescence measurements from living cells are correlated with the elevated reactive oxygen species concentrations and the cell cycle. Its uses can be in nondestructive detection of plant viral infections (or other stresses), growth rates and tumors.

Luminescence measurements from living cells are correlated with the elevated reactive oxygen species concentrations and the
Luminescence measurements from living cells are correlated with the elevated reactive oxygen species concentrations and the

The advantage of delayed fluorescence as well as luminescence measurements is the possibility of field measurements of intact organisms in situ as well as laboratory assays and studies.

The Innovation

There are numerous publications reporting the use of delayed fluorescence or luminescence for addressing different issues in physiology, ecology, agronomy and medicine. However, the methodology is still not standardised, nor there exists a versatile device for routine measurements.

There are numerous publications reporting the use of delayed fluorescence or luminescence for addressing different issues in physiology, ecology, agronomy and medicine. However, the methodology is still not standardised, nor there exists a versatile device for routine measurements.

Our proposal is to review all the uses of delayed fluorescence and luminescence in order to design a versatile tool for risk assessment and monitoring and to design and construct the necessary measuring equipment. The device based on light- emission detection from cells, tissues and organisms in the field or in the laboratory could then be used for a wide spectrum of environmental studies.

Our proposal is to review all the uses of delayed fluorescence and luminescence in order to design a versatile tool for risk assessment and monitoring and to design and construct the necessary measuring equipment. The device based on light- emission detection from cells, tissues and organisms in the field or in the laboratory could then be used for a wide spectrum of environmental studies.

Our proposal is to review all the uses of delayed fluorescence and luminescence in order to design a versatile tool for risk assessment and monitoring and to design and construct the necessary measuring equipment. The device based on light- emission detection from cells, tissues and organisms in the field or in the laboratory could then be used for a wide spectrum of environmental studies.

Our Contribution

Our main scientific interest is in studying light phenomena in living matter. For that purpose we designed and constructed a system for measuring weak photon emission from cells, tissues or whole organisms.

Our main scientific interest is in studying light phenomena in living matter. For that purpose we

Our main scientific interest is in studying light phenomena in living matter. For that purpose we designed and constructed a system for measuring weak photon emission from cells, tissues or whole organisms.

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We apply the system for measuring delayed fluorescence of algae and higher plants in order to detect stress and measure some parameters of photosynthesis.

We apply the system for measuring delayed fluorescence of algae and higher plants in order to

We have designed a rapid biotest for ecotoxicological screening of environmental contamination using the delayed fluorescence of Lemna minor and (a marine alga) Skeletonema costatum. The methodology will also be used in assessing the quality of soil, water, vegetation and crops for ecofarming or precision farming.

We have designed a rapid biotest for ecotoxicological screening of environmental contamination using the delayed fluorescence
We have designed a rapid biotest for ecotoxicological screening of environmental contamination using the delayed fluorescence

We have designed a rapid biotest for ecotoxicological screening of environmental contamination using the delayed fluorescence of Lemna minor and (a marine alga) Skeletonema costatum. The methodology will also be used in assessing the quality of soil, water, vegetation and crops for ecofarming or precision farming.

We have designed a rapid biotest for ecotoxicological screening of environmental contamination using the delayed fluorescence
We have designed a rapid biotest for ecotoxicological screening of environmental contamination using the delayed fluorescence

We perform delayed and prompt fluorescence measurements of marine algae to set up a system for monitoring the toxic algal populations and algal blooms

We perform delayed and prompt fluorescence measurements of marine algae to set up a system for

Expected Contribution of the Partners

ï joint research and development of the methodologies in the different fields of use and/or development of the necessary instrumentation

ï manufacturing and/or marketing of the proposed new technology

Thank You