Bioreactor Design – Fundamentals

The most widely used Industrial Bioreactors are: •Batch Reactors •Continuous Reactors •Continuous Reactors With Recycle •Semi Continuous Reactors (“Fill/Draw” Reactors including Sequencing Batch Reactors)

Bioreactor Design – Fundamentals (Cont’d)

DEFINITIONS “Limiting Substrate” The substrate or substrate(s) (energy source, carbon source and/or nutrient source) which is/are first exhausted in batch growth. This substrate(s) has a direct influence on the kinetics of cell growth.

Bioreactor Design – Fundamentals (Cont’d)

DEFINITIONS “In Excess” The substrate or substrate(s) (energy source, carbon source and/or nutrient source) which is/are NOT exhausted at the end of batch growth. This substrate(s) has NO EFFECT on the kinetics of cell growth.

Bioreactor Design – Fundamentals (Cont’d)

Bioeactor Design – Fundamentals (Cont’d) k1* and A are combined to form another constant k and the rate expression becomes: RATE = k (S / KS + S) Where k = maximum rate of transport of S ( mol / h) When S is much larger than KS : RATE ≈ k ≠ f (S) When S is similar to KS : RATE = f (S) .

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Bioreactor Design – Fundamentals (Cont’d) THE LAG PHASE .

Bioreactor Design – Fundamentals (Cont’d) THE LAG PHASE .

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Bioreactor Design Fundamentals .

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Bioreactor Design Fundamentals (Cont’d) .

Bioreactor Design Fundamentals (Cont’d) .