COLOR CHART

many as bubbEs

gel rises \ I to surface liquid below gel is cloudy indicator turns yellow

+-='

-

,,
-

-,

,

,

. -.

-

J

liquid above gel is clear indicator remains red or turns with few gas bubbles

el remains 'at bottom of tube
64-361 6-CC R 9/09

"LclMotte CHESTERTOWN MARYLAND 21620 COLIFORM INDICATOR TEST KIT 4-3616 .

Carefully read through the instructions completely before attempting to collect sample or run the test. The water sample is placed in test vials containing the special coliform indicating tablets and stored at room temperature for a predetermined time period. disposable 5-tube method to indicate the presence of Total Coliform Bacteriz in a drinking water supply. A sterile Water Sampling Bag containing a dechlorinating tablet is included for chlorine removal.The Model TC-5 is an easy-to-use. Read uol cautions on ~ n d ~ v ~ d conto~ners carefully. After the required storage period. CONTENTS Each kit contains 5 glass tubes. The test method and results closely parallel the standard Total Colitorm Multiple-Tube Presumptive Test (MPN) as outlined in Standard Methods for the Examination of Water and Wastewater. the vials are examined to determine the presence of coliform bacteria. Not to be used by children except under adult supewlslon . marked at the 10 mL level and containing one Coliform Test Tablet. that may be harmful if misused.

coliform bacteria when used with this kit.1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 I 1 1 1 1 1 I 1 1 1 1 1 1 1 1 1 I INTRODUCTION: The tablets in the Model TC-5 contain nutrients to support the growth of coliform bacteria. This gas will be trapped in the gelling substance and cause the gel to rise in the tube. Suggestion: A s the test requires a 44-48 hour incubation time. and a pH indicator. 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 I ~ m I I m m I 1 1 I 1 1 ~ 1 I 1 1 m STORAGE T h e unused kit should be stored at room temperature and QU: . a gelling substance. The pH indicator may change color from red to yellow as further evidence of coliiom bscteria activity. CHLORlbOE RESIDUAL PRECAUTIONS Water samples with chlorine residuals tend to suppress the E::. This product is to be used for water analysis ccrrs *_7r?1\-. A sterile Water Sampling Bag containir.2-: ? . sample at a t i ~ pzriod convenient to the user e to read the end result.2 1 :t:?'larinaring agent is provided : to collect the sample and neutralize any chlorine which rr. Keep away from children.resent in the water. m . 2. If coliform organisms are present in the sample. gas will be generated as a result of the bacteria metabolizing the nutrients in the tablet.i 2irect sunlight.TI-.

water spigot. 1. system sample valve. Allow tap (cold m t e r ) to run for 2 to 3 minutes or until the line is flushed. Do not touch bag opening or inner surface.1111111111111111111111IIIIIIII1IIII1I~1 SAMPLE COLLECTION & TEST PROCEDURE 1. 4. Soak cotton ball cr Fauze with household alcohol and wipe entire water outlet area of spigot. faucet or test valve. 3. Determine sarnyle source. 2. Caution: Do not allow the tablet to fall out of the bag.e. m . Tear off top of ba: a: sL:>red line and pull tabs outward to open bag. Pay particular attention to faucet aerator screens and mixers. faucet. Reduce tap wawr 'u c- 10 a rate that will fill the Water Sampling Bag slowly without splashing. etc.

Shake bag to dissolve tablet. 6. pull wire ends to close and whirl bag 3 complete revolutions.5. Remove all 5 tubes from the display package and remove caps NOTE: Do not remove tabkt from tube. tubes. Fill bag to 4 oz fill line. do not touch the inner surface of the caps. CAUTI0N:To avoid contamination. or handle tablet .

Tab!?: i bottom of t u k . If these storage conditions are not t followed preci5c:-:. NOTE: Do not ?iz. - 1 I 8. or shake tubes during the i:i designated inc. e Do not mix or shake tubes. rubes should now be standing vertic:::.:T. :l.7. Fold one tare n:rs =\yard to form a spout.3:. I I 9. .:zmprature. for 44-48 hours.85OF. x r Carefully till all 5 ~ ~ ' LO the 10 rnL line with water sample. Stand the car?: ~_rrr:ght place all 5 tubes into and the display rzcJ:r:t.:.1 time period. Unwhirl bay and rul' t a b ouni-ard to open bag. " n e ~ ' x caps tightly. ..a-:-l? tablet at the bottom of rhe j . .t:r remperature should be fairly constant and k 7 r t c ~ '72'.z:: 7.. : lie flat o n the tube. handle. 5 e results of the test may vary and may not be valid. out of direct sunlight. Store tubes at r.

8 will cause the indicator to turn yellow prematurely. Substrate below gel is cloudy. Gel rises to surface of sample.+ Many gas bubbles evident within gelling substance. Indicator turns yellow. Gel will float to surface of sample indicating gas formation. . Both the yellow color and gas bubbles must be evident in order to establish a positive test result.1 m 1 1 1 1 1 1 1 m 1 m m 1 1 1 1 m m 1 1 1 1 1 m 1 1 1 m w m m 1 1 a m m m 1 TEST RESULTS POSITIVE TEST . This is not indicative of a positive result. PNOTE: Some water samples with a pH below 6. Y -1 . Substrate below should be cloudy.

Indicator remains red or turns yellow with few gas bubbles. TNOTE: Some n-zrer samples with a pH below 6. T h i s is not indicative of a positive result.8 will cause the indicator to turn yellow prematurely.NEGATIVE TEST .? Gelling ~ubsrance remains on bottom of tube. .e gelling substance should be clear. Substrate a h .

Let tubes stand for approximately 4 hours. . After results are recorded. . Dispose of tubes and package as required by local jurisdiction. O n e tube at a time. Further steps should be taken to validate these results by a certified bacteriological laboratory. Follow the above procedure for all 5 tubes. Do not open tubes or attempt to clean for reuse. there is a potential for a serious bacterial contamination problem. . Return tubes to foam tray and again stand upright. remove cap and add approximately 1 mL (or 113 teaspoon) of household bleach (chlorine bleach) to the tube and immediately recap. dispose of the inoculated tubes as follows: Remove all 5 tubes from display package. If a positive result is found in two or more tubes.RESULTS ANALYSIS A positive result in any one of the five tubes should be regarded as a potential coliform bacteria contamination.