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leaf extracts on European Red Mite, Panonychus ulmi and its predator Stethorus gilvifrons Nabi Alper Kumral1, Sultan Çobanoğlu2, Cem Yalçın1
Department of Plant Protection, Faculty of Agriculture, Uludag University, Gorukle Campus, Bursa 16059, Turkey 2 Department of Plant Protection, Faculty of Agriculture, Ankara University, Diskapi, Ankara, 06110, Turkey
corresponds to.9.95, 39.81, 69.67, 99.59, 129.38, 159.24 l/l-1air) in five replications under laboratory conditions (25+ 2 ˚C and 55+ 5 %RH). Results showed, the most toxicity essential oil was T. minuta , as its lethal concentration, LC50= 11.60, LC99=768.35 lL-1air. M. comunis essential oil had the least toxic effects against B. brassica, as its LC50 = 78.58 and LC99 =367.24 lL-1air. The results of the present study concluded that these plant essential oils have the potential to provide efficient and safer insecticide activity for human and the environment.
The ethanol extracts obtained from leaf in the thorn apple (Datura stramonium L.) (Solanaceae) were investigated for adult mortality and repellent properties against both European red mite [Panonychus ulmi (Koch)] (Acari: Tetranychidae) and its predator Stethorus gilvifrons (Muls.) (Col.: Coccinellidae) under laboratory conditions. The current study demonstrated that leaf extracts of D. stramonium had lethal effects on both the spider mite and lady-bird using a Petri leaf disc-spray tower method. Furthermore, an increase in the dose of leaf extract was associated with a significant increase in the death rate of both P. ulmi and S. gilvifrons adults. Using probit analysis and estimating the parameters, the LC50 values of leaf extract were determined to be 7097.5 and 5427.5 mg/l at 24h residual activity for spider mite and lady-bird adults, respectively. In addition, the mite and ladybird mortality by the extract was higher at 48h than that at 24 h, with 2013 and 1030 mg/l LC50 values, respectively. But, the LC90 value of the extract at both 24h and 48h was lower to lady-bird adults compared with spider mite adults. According to Pearson’s chi-squared test, both spider mites and lady-birds adults showed the strongest run off to apple leaf surfaces sprayed with the extracts in sub-lethal doses (from 13.72 to 1058.0 mg/l). Moreover, the odour test using two-choice olfactometer demonstrated the lady-bird adults exhibited a significant going towards to clean air instead of the extract odour. These results suggest that D. stramonium extract can effectively be utilized as safer phytopesticidial products for manage P. ulmi in both organic and inorganic apple growing, but it is not suitable for Integrated Pest Management strategies due to its toxic and repellent effects to S. gilvifrons.
TU 35 Insecticidal activity of five essential oils against Myzus persicae (Sulzer) (Hemiptera: Aphididae) Azadeh Davoodi 1, Maryam Aleosfoor 2, Najmeh Motazedian 3, Mohammad Sadegh Sadeghi 1, Mohammad Jamal Saharkhiz 4
1 Plant Virology Research Center, College of Agriculture, Shiraz University, Shiraz, Iran. 2 Department of Entomology, Faculty of Agriculture, Shiraz University, Shiraz, Iran 3 Plant Protection Center of Fars Province, Shiraz, Iran 4 Department of Horticultural sciences, Faculty of Agriculture, Shiraz University, Shiraz, Iran
TU 34 Screening of insecticidal activity of nine medicinal essential oils against Brevicoryne brassica L. (Hemiptera: Aphididae) Najmeh Motazedian1, Azadeh Davoodi2, Maryam Aleosfoor3, Mohammad Jamal Saharkhiz4, Mohammad Sadegh Sadeghi2
Plant Protection Center of Fars Province, Shiraz, Iran Plant Virology Research Center, College of Agriculture, Shiraz University, Shiraz, Iran. 3 Department of Entomology, Faculty of Agriculture, Shiraz University, Shiraz, Iran 4 Department of Horticultural sciences, Faculty of Agriculture, Shiraz University, Shiraz, Iran
Myzus persicae (Sulzer) (Hemiptera: Aphididae) is one of the most important aphids in Iran. This insect causes both direct and indirect damages. Screening for pesticidal activity of plant essential oils with some known medicinal attributes could lead to discovery new agent pesticides. The current study was carried out to find safe aphidicides for controlling green peach aphid. So essential oils extracted from five aromatic plants, Tagetes minuta (Asteraceae) , Trachyspermum ammi (Apiaceae), Artemisia siberi (Asteraceae), Zataria multiflora (Lamiaceae) and Nepeta cataria (Lamiaceae), grown in Iran by Using a Clevenger type apparatus, then the oils were analyzed by gas chromatography– mass spectrometry (GC–MS) and evaluated for insecticide activity against adult M. persicae by fumigant toxicity method in 25+ 2 ˚C and 55+ 5 %RH with five replications. After 24h of toxicity bioassay, using five doses (0.25, 0.5, 1, 2and 4 l corresponding to 3.9, 7.9, 15.7, 31.5 and 62.9 lL-1air) indicates that for all treatments, mortality increases when concentration exceeded. Results showed, the most toxicity caused by Trachyspermum ammi essential oil (LC50= 5.28 and LC99= 75.60 lL-1air). Tagetes minuta essential oil had the least toxic effect on M. persicae (LC50= 9.44 LC99 =135.33 lL-1air). The results concluded that these plant essential oils have potential to be used in promoting research aiming at the development of new pesticides from the plants with medicinal values.
Essential oils are volatile mixtures of hydrocarbons with a diversity of functional groups and their insecticide activity has been linked to the presence of monoterpenes and sesquiterpens. The present abstract discuses this environmentally benign pest control using nine aromatic plant oils, Salvia officialis, Mentha longifolia , Zataria multiflora and Nepeta cataria from Lamiaceae, Tagetes minuta and Artemisia siberi from Asteraceae, Trachyspermum ammi from Apiaceae and Myrtus comunis from Myrtaceae family against adult of cabbage aphid, Brevicoryne brassica L. (Hemiptera: Aphididae). The fumigant toxicities of the oils were assessed at 5 rates for Z. multiflora, N. cataria, T. minuta, T. ammi and A. siberi (0.25, 0.5, 1, 2and 4 l corresponding to 3.9, 7.9, 15.7, 31.5 and 62.9 lL-1air) and 6 rates for S. officialis, M. longifolia and M. comunis (0.5, 2, 3.5, 5, 6.5, 8 l of essential oils which
TU 36 Effect of five essential oils against two aphids, Aphis gossypii Glover and Schizaphis graminum Rondani (Hemiptera: Aphididae) under laboratory conditions Maryam Aleosfoor1, Mohammad Jamal Saharkhiz2, Najmeh Motazedian3, Azadeh Davoodi4, Mohammad Sadegh Sadeghi4
1 Department of Entomology, Faculty of Agriculture, Shiraz University, Shiraz, Iran 2 Department of Horticultural sciences, Faculty of Agriculture, Shiraz University, Shiraz, Iran 3 Plant Protection Center of Fars Province, Shiraz, Iran 4 Plant Virology Research Center, College of Agriculture, Shiraz University, Shiraz, Iran
The use of natural products raises several concerns related to environment and human health. Among these products, essential oils from plants have been extensively tested for having repellent and toxic activities. In this survey the effect of five essential oils extracted from Tagetes minuta